CSPI03G21620.1 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI03G21620.1
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	Reverse transcriptase
Location	Chr3: 17915161 .. 17915768 (+)
Sequence length	369
RNA-Seq Expression	CSPI03G21620.1
Synteny	CSPI03G21620.1

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAATATGATAGGTTGTGTGTCCCTAGAAACCAAGGCATAAAAGATCAAATTTTAGAGGAAGCATATAGTTCTGCATATGATATTCATTCTGGCAGTACAAAGATGTATAGAACTTTAAAGAAGCACTATTGGTGGCTTGGTATGAAGCGTGAAACTGCAGAATATGTAGTTAAGTGTTTAATCTATCAACAAGTTAAACCTGAATGATAGATAGCAGCAGAAATTTTAAACGTACTTCTAGTGCTGAAATGGAAATGGAAACATGTTACCATGGACTTTTTATTTGGACTTCCTCGCACATCGAGTGGGTTTGATGGCATTTGGGTGATAGTTGACAGACTTACAAAGACAACAAGATTTTGACTAGTGAAGGTCACTTTCACCTTCGATAAGTTGGCCAAATTGTAGATTAGTAAGATTGTAAGTGCGAATGGAGCTCCCGTATCTATTATGTCTAATAGAGATTCCAAGTTTACCCTAAAGTTTTGTCTGAGTTTGCAACAAGTAATGGGCACCAATTTACATTTCAGTACCAGATTTCGTCCTCTGATATATTGTCAATCATAAAGAACCAAGACCTTGGAAGATATGCTTCAAGCTTGTGA

mRNA sequence

ATGAAATATGATAGGTTGTGTGTCCCTAGAAACCAAGGCATAAAAGATCAAATTTTAGAGGAAGCATATAGTTCTGCATATGATATTCATTCTGGCAGTACAAAGATGTATAGAACTTTAAAGAAGCACTATTGGTGGCTTGTGCTGAAATGGAAATGGAAACATGTTACCATGGACTTTTTATTTGGACTTCCTCGCACATCGAGTGGGTTTGATGGCATTTGGAGATTCCAAGTTTACCCTAAAGTTTTGTCTGAGTTTGCAACAAGTAATGGGCACCAATTTACATTTCAGTACCAGATTTCGTCCTCTGATATATTGTCAATCATAAAGAACCAAGACCTTGGAAGATATGCTTCAAGCTTGTGA

Coding sequence (CDS)

ATGAAATATGATAGGTTGTGTGTCCCTAGAAACCAAGGCATAAAAGATCAAATTTTAGAGGAAGCATATAGTTCTGCATATGATATTCATTCTGGCAGTACAAAGATGTATAGAACTTTAAAGAAGCACTATTGGTGGCTTGTGCTGAAATGGAAATGGAAACATGTTACCATGGACTTTTTATTTGGACTTCCTCGCACATCGAGTGGGTTTGATGGCATTTGGAGATTCCAAGTTTACCCTAAAGTTTTGTCTGAGTTTGCAACAAGTAATGGGCACCAATTTACATTTCAGTACCAGATTTCGTCCTCTGATATATTGTCAATCATAAAGAACCAAGACCTTGGAAGATATGCTTCAAGCTTGTGA

Protein sequence

MKYDRLCVPRNQGIKDQILEEAYSSAYDIHSGSTKMYRTLKKHYWWLVLKWKWKHVTMDFLFGLPRTSSGFDGIWRFQVYPKVLSEFATSNGHQFTFQYQISSSDILSIIKNQDLGRYASSL*

Homology

[Close]

BLAST of CSPI03G21620.1 vs. ExPASy TrEMBL
Match: A0A5D3D2Y2 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold306G004160 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 1.1e-23
Identity = 56/78 (71.79%), Postives = 66/78 (84.62%), Query Frame = 0


Query: 1   MKYDRLCVPRNQGIKDQILEEAYSSAYDIHSGSTKMYRTLKKHYWWLVLKWK---WKHVT 60
           MKYDRLCVP +Q IKDQI EEA+SSAY +H GSTK+YRTLKKHYWW  +K +   ++HVT
Sbjct: 602 MKYDRLCVPSDQTIKDQISEEAHSSAYAMHPGSTKVYRTLKKHYWWPGMKREIADYEHVT 661

Query: 61  MDFLFGLPRTSSGFDGIW 76
           MDFLFGLPRT++G DGIW
Sbjct: 662 MDFLFGLPRTTNGNDGIW 679

[Close]

BLAST of CSPI03G21620.1 vs. ExPASy TrEMBL
Match: A0A5A7TKQ0 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold67G006300 PE=4 SV=1)

HSP 1 Score: 119.4 bits (298), Expect = 1.1e-23
Identity = 56/78 (71.79%), Postives = 66/78 (84.62%), Query Frame = 0


Query: 1   MKYDRLCVPRNQGIKDQILEEAYSSAYDIHSGSTKMYRTLKKHYWWLVLKWK---WKHVT 60
           MKYDRLCVP +Q IKDQI EEA+SSAY +H GSTK+YRTLKKHYWW  +K +   ++HVT
Sbjct: 602 MKYDRLCVPSDQTIKDQISEEAHSSAYAMHPGSTKVYRTLKKHYWWPGMKREIADYEHVT 661

Query: 61  MDFLFGLPRTSSGFDGIW 76
           MDFLFGLPRT++G DGIW
Sbjct: 662 MDFLFGLPRTTNGNDGIW 679

[Close]

BLAST of CSPI03G21620.1 vs. ExPASy TrEMBL
Match: A0A5D3DCU0 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold305G00110 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 4.0e-23
Identity = 59/109 (54.13%), Postives = 67/109 (61.47%), Query Frame = 0


Query: 1   MKYDRLCVPRNQGIKDQILEEAYSSAYDIHSGSTKMYRTLKKHYWW-------------- 60
           MKYDRLCVP +Q IK QIL+EA+SSAY +H GSTKMYRTLKKHYWW              
Sbjct: 99  MKYDRLCVPSDQAIKVQILKEAHSSAYAMHPGSTKMYRTLKKHYWWPTMKCEIADYVAKC 158

Query: 61  --------------------LVLKWKWKHVTMDFLFGLPRTSSGFDGIW 76
                                VL+WKW+HVTMDFLFGLPRT+ G +GIW
Sbjct: 159 LICQQVKPERQRPAELLNPLPVLEWKWEHVTMDFLFGLPRTTIGHEGIW 207

[Close]

BLAST of CSPI03G21620.1 vs. ExPASy TrEMBL
Match: A0A5A7SQ03 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G002850 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 4.0e-23
Identity = 59/109 (54.13%), Postives = 67/109 (61.47%), Query Frame = 0


Query: 1   MKYDRLCVPRNQGIKDQILEEAYSSAYDIHSGSTKMYRTLKKHYWW-------------- 60
           MKYDRLCVP +Q IK QIL+EA+SSAY +H GSTKMYRTLKKHYWW              
Sbjct: 99  MKYDRLCVPSDQAIKVQILKEAHSSAYAMHPGSTKMYRTLKKHYWWPTMKCEIADYVAKC 158

Query: 61  --------------------LVLKWKWKHVTMDFLFGLPRTSSGFDGIW 76
                                VL+WKW+HVTMDFLFGLPRT+ G +GIW
Sbjct: 159 LICQQVKPERQRPAELLNPLPVLEWKWEHVTMDFLFGLPRTTIGHEGIW 207

[Close]

BLAST of CSPI03G21620.1 vs. ExPASy TrEMBL
Match: A0A5D3DXH2 (DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold384G001290 PE=4 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 1.2e-22
Identity = 66/136 (48.53%), Postives = 81/136 (59.56%), Query Frame = 0


Query: 1   MKYDRLCVPRNQGIKDQILEEAYSSAYDIHSGSTKMYRTLKKHYWWLVLK---------- 60
           +K  RLCVP    +KD ILEEA++SAY IH GSTKMYRTLKK YWW V +          
Sbjct: 20  VKQGRLCVPNISELKDAILEEAHNSAYAIHLGSTKMYRTLKKTYWWPVRQRPGGLLNPLP 79

Query: 61  ---WKWKHVTMDFLFGLPRTSSGFDGIWRFQVYPKVLSEF----ATSNGHQFTFQYQ--- 116
              WKW+H+TMDFLFGLPRTS G+DGIW     P   + F    ATS   Q    Y    
Sbjct: 80  VPDWKWEHITMDFLFGLPRTSRGYDGIWVIVDRPTKTARFIPIKATSTLDQLAKLYVDRI 139

[Close]

BLAST of CSPI03G21620.1 vs. NCBI nr
Match: TYK18080.1 (hypothetical protein E5676_scaffold306G004160 [Cucumis melo var. makuwa])

HSP 1 Score: 119.4 bits (298), Expect = 2.2e-23
Identity = 56/78 (71.79%), Postives = 66/78 (84.62%), Query Frame = 0


Query: 1   MKYDRLCVPRNQGIKDQILEEAYSSAYDIHSGSTKMYRTLKKHYWWLVLKWK---WKHVT 60
           MKYDRLCVP +Q IKDQI EEA+SSAY +H GSTK+YRTLKKHYWW  +K +   ++HVT
Sbjct: 602 MKYDRLCVPSDQTIKDQISEEAHSSAYAMHPGSTKVYRTLKKHYWWPGMKREIADYEHVT 661

Query: 61  MDFLFGLPRTSSGFDGIW 76
           MDFLFGLPRT++G DGIW
Sbjct: 662 MDFLFGLPRTTNGNDGIW 679

[Close]

BLAST of CSPI03G21620.1 vs. NCBI nr
Match: KAA0042141.1 (hypothetical protein E6C27_scaffold67G006300 [Cucumis melo var. makuwa])

HSP 1 Score: 119.4 bits (298), Expect = 2.2e-23
Identity = 56/78 (71.79%), Postives = 66/78 (84.62%), Query Frame = 0


Query: 1   MKYDRLCVPRNQGIKDQILEEAYSSAYDIHSGSTKMYRTLKKHYWWLVLKWK---WKHVT 60
           MKYDRLCVP +Q IKDQI EEA+SSAY +H GSTK+YRTLKKHYWW  +K +   ++HVT
Sbjct: 602 MKYDRLCVPSDQTIKDQISEEAHSSAYAMHPGSTKVYRTLKKHYWWPGMKREIADYEHVT 661

Query: 61  MDFLFGLPRTSSGFDGIW 76
           MDFLFGLPRT++G DGIW
Sbjct: 662 MDFLFGLPRTTNGNDGIW 679

[Close]

BLAST of CSPI03G21620.1 vs. NCBI nr
Match: KAA0026281.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 117.5 bits (293), Expect = 8.3e-23
Identity = 59/109 (54.13%), Postives = 67/109 (61.47%), Query Frame = 0


Query: 1   MKYDRLCVPRNQGIKDQILEEAYSSAYDIHSGSTKMYRTLKKHYWW-------------- 60
           MKYDRLCVP +Q IK QIL+EA+SSAY +H GSTKMYRTLKKHYWW              
Sbjct: 99  MKYDRLCVPSDQAIKVQILKEAHSSAYAMHPGSTKMYRTLKKHYWWPTMKCEIADYVAKC 158

Query: 61  --------------------LVLKWKWKHVTMDFLFGLPRTSSGFDGIW 76
                                VL+WKW+HVTMDFLFGLPRT+ G +GIW
Sbjct: 159 LICQQVKPERQRPAELLNPLPVLEWKWEHVTMDFLFGLPRTTIGHEGIW 207

[Close]

BLAST of CSPI03G21620.1 vs. NCBI nr
Match: TYK21477.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 117.5 bits (293), Expect = 8.3e-23
Identity = 59/109 (54.13%), Postives = 67/109 (61.47%), Query Frame = 0


Query: 1   MKYDRLCVPRNQGIKDQILEEAYSSAYDIHSGSTKMYRTLKKHYWW-------------- 60
           MKYDRLCVP +Q IK QIL+EA+SSAY +H GSTKMYRTLKKHYWW              
Sbjct: 99  MKYDRLCVPSDQAIKVQILKEAHSSAYAMHPGSTKMYRTLKKHYWWPTMKCEIADYVAKC 158

Query: 61  --------------------LVLKWKWKHVTMDFLFGLPRTSSGFDGIW 76
                                VL+WKW+HVTMDFLFGLPRT+ G +GIW
Sbjct: 159 LICQQVKPERQRPAELLNPLPVLEWKWEHVTMDFLFGLPRTTIGHEGIW 207

[Close]

BLAST of CSPI03G21620.1 vs. NCBI nr
Match: KAA0066793.1 (DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa] >TYK27940.1 DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa])

HSP 1 Score: 115.9 bits (289), Expect = 2.4e-22
Identity = 66/136 (48.53%), Postives = 81/136 (59.56%), Query Frame = 0


Query: 1   MKYDRLCVPRNQGIKDQILEEAYSSAYDIHSGSTKMYRTLKKHYWWLVLK---------- 60
           +K  RLCVP    +KD ILEEA++SAY IH GSTKMYRTLKK YWW V +          
Sbjct: 20  VKQGRLCVPNISELKDAILEEAHNSAYAIHLGSTKMYRTLKKTYWWPVRQRPGGLLNPLP 79

Query: 61  ---WKWKHVTMDFLFGLPRTSSGFDGIWRFQVYPKVLSEF----ATSNGHQFTFQYQ--- 116
              WKW+H+TMDFLFGLPRTS G+DGIW     P   + F    ATS   Q    Y    
Sbjct: 80  VPDWKWEHITMDFLFGLPRTSRGYDGIWVIVDRPTKTARFIPIKATSTLDQLAKLYVDRI 139

The following BLAST results are available for this feature:

BLAST of CSPI03G21620.1 vs.


Match Name	E-value	Identity	Description

BLAST of CSPI03G21620.1 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D2Y2	1.1e-23	71.79	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A5A7TKQ0	1.1e-23	71.79	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A5D3DCU0	4.0e-23	54.13	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold30...	[more]
A0A5A7SQ03	4.0e-23	54.13	Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19...	[more]
A0A5D3DXH2	1.2e-22	48.53	DNA/RNA polymerases superfamily protein OS=Cucumis melo var. makuwa OX=1194695 G...	[more]

BLAST of CSPI03G21620.1 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK18080.1	2.2e-23	71.79	hypothetical protein E5676_scaffold306G004160 [Cucumis melo var. makuwa]	[more]
KAA0042141.1	2.2e-23	71.79	hypothetical protein E6C27_scaffold67G006300 [Cucumis melo var. makuwa]	[more]
KAA0026281.1	8.3e-23	54.13	reverse transcriptase [Cucumis melo var. makuwa]	[more]
TYK21477.1	8.3e-23	54.13	reverse transcriptase [Cucumis melo var. makuwa]	[more]
KAA0066793.1	2.4e-22	48.53	DNA/RNA polymerases superfamily protein [Cucumis melo var. makuwa] >TYK27940.1 D...	[more]

BLAST of CSPI03G21620.1 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	1.10.340.70		coord: 1..58 e-value: 5.8E-7 score: 31.7

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI03G21620	CSPI03G21620	gene

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI03G21620.1.cds1	CSPI03G21620.1.cds1	CDS
CSPI03G21620.1.cds2	CSPI03G21620.1.cds2	CDS
CSPI03G21620.1.cds3	CSPI03G21620.1.cds3	CDS

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI03G21620.1	CSPI03G21620.1-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
molecular_function	GO:0003676	nucleic acid binding