CSPI02G00860.3 (mRNA) Cucumber (PI 183967) v1

Overview


Name	CSPI02G00860.3
Type	mRNA
Organism	Cucumis sativus var. hardwickii cv. PI 183967 (Cucumber (PI 183967) v1)
Description	tRNA A64-2'-O-ribosylphosphate transferase-like
Location	Chr2: 512031 .. 515483 (+)
Sequence length	1742
RNA-Seq Expression	CSPI02G00860.3
Synteny	CSPI02G00860.3

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GATTTATCCATGGAGGACGGCGCGAAGCTGAGCATATACAAGGCCATCAGAAACATAAAACGTAGAGATAATACTCTCTACAACGCTCTCAGATCAATTTACGAGGACTCTATTTTCGTCGGCGAGATCGCTCAGCTATGGCCGGAGCTTCCTCTTCTCGCAAATCTCCGTTGCGGCCTTTGGTATTCCCAGAAGTTTCACTCCAATTGCTACTTCAAGTCCACCGACGGCCACAACAACAACTGGTCTTTCAGTACTTCCCGCCTCAATCTTCATGTGGCGCAATTGGCTGGTACATCCTCTCTTTTTCCCTGTCTGTTTTTCTTTTATTTTTCTAGCGAATAAGCTGCTTGAATCATGACAAATAATTACAGACTGTAGAATTTTCTGTATTGGCTTATTAATTTTGCCGACTTTGAATTCTCAAGGAATGCATCGGAGGATTGTTTTACATTTTTGAAGTGTTTATGTGATTCGCTTTTTTGTTTGATGGGTAGGACAGAAGGGAGGATGTTTTATAGTTGATTCCACTCGAAGAGGAAAGCGCTTTCCTGATAGCATGTCCAAAACAATACCCATTTGGACTTGTGTTTTGAATCGGTCTATAAGCAAACATTTGAACAAAATCCACGGCAGTTCATTGGAGCTTGGGGTGAGTATTGGCTATTTTGTTCTTTGCTTTTTCATCATTTGGCAGTCTATGATTCTGTGCAGGTCTAGGATCAAGAATTCACTCCCTCTAAGTCGGGTGTTAGTCTGAAATTGATTGAACAAATCTGTTTGATGGATCAGCAAGTAGTTTGAATTGATCGACCTAAACTGACCCGTTTACATCTCTATCTCAAGGGTGAAACTCTAAATGAGAAAGTTCATACATTTTATGTGTGGCATGTCTTTTGTAACAGGAGCCTTCCATTTCTGATGTGCTTGCTGAAAATGGAAAGGAATGTGCTGTTGGTTGGGACTGTTCCTTGCATCTACCCCTTTGGGTTTCTGAGACCGAGAAAGCTGCTATTGAGGAACGTCTTGAAGAATGGACGAAACAGTTAGAGGACTGTGGAGCTGATGTTGCCACATTGGCATCAATCTTGAGAAAGCCATTGCGCCCCTTGTGGATTTCACAAAGATCGGTTATTTGGTTGAACGAAGTACCAGATCATGACTCCTGGGATTTCACCCCCATTATACTTGTTAATGCTTCGTCCTCTACTGGTGTGGTTCAACAAAGGACCACCACAGAGTTTAGCTGGAACTACATTTCTGGAGCAGGAGACGATGAAGAAAGTTGGGCAAGAGGCTTGACTCCAAATCTGTTTTGGAAGCACGCACATGATCTTATGAGCTCTGGGCCTGATGTTTGTAATCAGAAGGTAGCTGAAATAGTTGAAAAGGACAGAGTATATCGAGCACTAAGGGGAAATGATGCACCTCAGATCAGTGTTAAATCTCAGAAAGGATCAACTCAAGTCCCGCCTATAGAGCAAATGTTATCTTTTGATATTTCTGATGCTGAGCTAGATGTAAAGTCTTCTGACGGAAATTGTGGATTATCTTGGCTTGGTTTAACTAAACTTGCAGTAGGCTCATCTCAATATGGTATTTACCTCCAATTTCTGTTGTGTTAAGGATAATACAGTAGATGATTATTGGTTTAGTATAGAGTTGAATGCTTTCTGTATCAATATTGGAAAAGAAGGTTCAAAAGGAAAGAAAATATAAAGCCTGGCTACTTGTTTTTCCGCTAAAGATCGTCTTTGTCTTAATAATTGAAAGGTTAACCATATTTCAAGAAATAAAATGCAATATTCAACATCTGTAAAAGTGATCAATGATTGTTTTACATGAGAAAATATTTCCAATCTTAGCCATTTTCCAAATTCACTTTCGACTATTCTTGGTTTTCCTGTGGAAATATTATTAGTTTGGCAACAAATGTAGGAGTGTATTTTATGCGGTTCACTAATTACATTATTGTTTCTTGACATCACTTATCTGATGATTACTTTTGTTTTATGTGTGTGCAGCTGTGACTGCGTGCGATGTTGATTGCATTTTAAATTGCGATCAAGAGACCTTATCTGTTTGCCTTCCAGTTGCTAGTGCATATCTTCATCTACCTTTGGTGGTGAGTATGGGAAAATGAGGGTGTGACCATTCTTTAGTTTGCAGCACCTAATTCAACTTATTTAACAGCTTTTGCTTTTCAGATGTCAAAAATGGATCGATTTTCCCTGTCGCGAAACCTTGCTTCTGCCGTAGATTTTGCAAAGTTGAATCTTAACCAAGGGAAAAGACTTCTAGTTTGCTGTAGCAATGGTAAGAACACCCTTTATTTAACTATAACTACAATAAACTCACCTATTTGAGATGAAAATCTACTGAAGAGGGTTGGACAGATTAGGGGAAGTGTTAGGTCTCTTCTTTTGAGTAGCTGGGCTAATCGCTCTTTGTAACTTTCTTTAAGTTATTTATTTAGTCAGTAATATGGTGTATGAAGATGTCAAGCATGAGTTGATGCAACATTTCTGAAACTTTTGCTAAGAGATCTCTTTTGTTTACTTGCAGGAAAAGATATTAGCATATGTGTTTGTTTGGCAATACTGACATCTTTATTTGACGAAGAAGGTACCCTGGCTGAATCTTTTTGTGTTAAACTTTCTTAAAGTACACCAAATGCATACATTTATGCTCTTTTCATCTTTTCATCTGCCCTTCAGCCCCTTCCTCTATTAGAACTTAGAAGGAGAGTTGTTAATTTATCATTTCCTTTTAGGGATTTTGGATATTTTAAGATGTAAGGGTGTTTTTTTTTGGAAAATGAGTCTTCAGCAAAATTTGACTCAAAATGTGAAGTTGAAAAGGAGGTTTTAAATACTCAATCCCTTCTAGTAAATTTTGTGTTAAATTAATGTAGAAGTGTTGTTTTTCACACCTGAGAAGAGGAACTCTTTTTTCAATTTCAAATTCAATTTAAGTCTCTCAGCACCTCTTCTCCAAGAGAGAAAAAGAGAGCAATTATCTTCCTACTTGTACAATTTTTTTTATTTCAAAGAATAGAAGGAATATGTTGCTTTCATGTATTAGTTCATTAGTAACTGTGTTAAATTACACCAGGAACTTTCGATTGCGGGAAATCTTTTAACAACACGCTTATTACAAAATTGGAAATGAAAAGACGACTCGTATACATCTGTAAATTTGCAACAAATGCAAGGCCTTCCAGAGGAAATTTGAGGCAGGTTTTCAATTTTCTTAGTGGAGGAAGAACTGATTAACTACAGCAAAGCAGCTTCAACAACTTTATAGGTTTTTCCTTTTACTTTTTATTATTCTTTTTCTTTTACTATCTTTTACCTTTTATACTGTTGAAGTTGATAAGGCCAAGATCCCCTGATACTTTGAAGATATTGCTTGTTCTTAATTATTTTCTTAAAATGAAACCAAGTTTGTGGG

mRNA sequence

GATTTATCCATGGAGGACGGCGCGAAGCTGAGCATATACAAGGCCATCAGAAACATAAAACGTAGAGATAATACTCTCTACAACGCTCTCAGATCAATTTACGAGGACTCTATTTTCGTCGGCGAGATCGCTCAGCTATGGCCGGAGCTTCCTCTTCTCGCAAATCTCCGTTGCGGCCTTTGGTATTCCCAGAAGTTTCACTCCAATTGCTACTTCAAGTCCACCGACGGCCACAACAACAACTGGTCTTTCAGTACTTCCCGCCTCAATCTTCATGTGGCGCAATTGGCTGAAGGGAGGATGTTTTATAGTTGATTCCACTCGAAGAGGAAAGCGCTTTCCTGATAGCATGTCCAAAACAATACCCATTTGGACTTGTGTTTTGAATCGGTCTATAAGCAAACATTTGAACAAAATCCACGGCAGTTCATTGGAGCTTGGGGAGCCTTCCATTTCTGATGTGCTTGCTGAAAATGGAAAGGAATGTGCTGTTGGTTGGGACTGTTCCTTGCATCTACCCCTTTGGGTTTCTGAGACCGAGAAAGCTGCTATTGAGGAACGTCTTGAAGAATGGACGAAACAGTTAGAGGACTGTGGAGCTGATGTTGCCACATTGGCATCAATCTTGAGAAAGCCATTGCGCCCCTTGTGGATTTCACAAAGATCGGTTATTTGGTTGAACGAAGTACCAGATCATGACTCCTGGGATTTCACCCCCATTATACTTGTTAATGCTTCGTCCTCTACTGGTGTGGTTCAACAAAGGACCACCACAGAGTTTAGCTGGAACTACATTTCTGGAGCAGGAGACGATGAAGAAAGTTGGGCAAGAGGCTTGACTCCAAATCTGTTTTGGAAGCACGCACATGATCTTATGAGCTCTGGGCCTGATGTTTGTAATCAGAAGGTAGCTGAAATAGTTGAAAAGGACAGAGTATATCGAGCACTAAGGGGAAATGATGCACCTCAGATCAGTGTTAAATCTCAGAAAGGATCAACTCAAGTCCCGCCTATAGAGCAAATGTTATCTTTTGATATTTCTGATGCTGAGCTAGATGTAAAGTCTTCTGACGGAAATTGTGGATTATCTTGGCTTGGTTTAACTAAACTTGCAGTAGGCTCATCTCAATATGCTGTGACTGCGTGCGATGTTGATTGCATTTTAAATTGCGATCAAGAGACCTTATCTGTTTGCCTTCCAGTTGCTAGTGCATATCTTCATCTACCTTTGGTGATGTCAAAAATGGATCGATTTTCCCTGTCGCGAAACCTTGCTTCTGCCGTAGATTTTGCAAAGTTGAATCTTAACCAAGGGAAAAGACTTCTAGTTTGCTGTAGCAATGGAAAAGATATTAGCATATGTGTTTGTTTGGCAATACTGACATCTTTATTTGACGAAGAAGGAACTTTCGATTGCGGGAAATCTTTTAACAACACGCTTATTACAAAATTGGAAATGAAAAGACGACTCGTATACATCTGTAAATTTGCAACAAATGCAAGGCCTTCCAGAGGAAATTTGAGGCAGGTTTTCAATTTTCTTAGTGGAGGAAGAACTGATTAACTACAGCAAAGCAGCTTCAACAACTTTATAGGTTTTTCCTTTTACTTTTTATTATTCTTTTTCTTTTACTATCTTTTACCTTTTATACTGTTGAAGTTGATAAGGCCAAGATCCCCTGATACTTTGAAGATATTGCTTGTTCTTAATTATTTTCTTAAAATGAAACCAAGTTTGTGGG

Coding sequence (CDS)

ATGTGGCGCAATTGGCTGAAGGGAGGATGTTTTATAGTTGATTCCACTCGAAGAGGAAAGCGCTTTCCTGATAGCATGTCCAAAACAATACCCATTTGGACTTGTGTTTTGAATCGGTCTATAAGCAAACATTTGAACAAAATCCACGGCAGTTCATTGGAGCTTGGGGAGCCTTCCATTTCTGATGTGCTTGCTGAAAATGGAAAGGAATGTGCTGTTGGTTGGGACTGTTCCTTGCATCTACCCCTTTGGGTTTCTGAGACCGAGAAAGCTGCTATTGAGGAACGTCTTGAAGAATGGACGAAACAGTTAGAGGACTGTGGAGCTGATGTTGCCACATTGGCATCAATCTTGAGAAAGCCATTGCGCCCCTTGTGGATTTCACAAAGATCGGTTATTTGGTTGAACGAAGTACCAGATCATGACTCCTGGGATTTCACCCCCATTATACTTGTTAATGCTTCGTCCTCTACTGGTGTGGTTCAACAAAGGACCACCACAGAGTTTAGCTGGAACTACATTTCTGGAGCAGGAGACGATGAAGAAAGTTGGGCAAGAGGCTTGACTCCAAATCTGTTTTGGAAGCACGCACATGATCTTATGAGCTCTGGGCCTGATGTTTGTAATCAGAAGGTAGCTGAAATAGTTGAAAAGGACAGAGTATATCGAGCACTAAGGGGAAATGATGCACCTCAGATCAGTGTTAAATCTCAGAAAGGATCAACTCAAGTCCCGCCTATAGAGCAAATGTTATCTTTTGATATTTCTGATGCTGAGCTAGATGTAAAGTCTTCTGACGGAAATTGTGGATTATCTTGGCTTGGTTTAACTAAACTTGCAGTAGGCTCATCTCAATATGCTGTGACTGCGTGCGATGTTGATTGCATTTTAAATTGCGATCAAGAGACCTTATCTGTTTGCCTTCCAGTTGCTAGTGCATATCTTCATCTACCTTTGGTGATGTCAAAAATGGATCGATTTTCCCTGTCGCGAAACCTTGCTTCTGCCGTAGATTTTGCAAAGTTGAATCTTAACCAAGGGAAAAGACTTCTAGTTTGCTGTAGCAATGGAAAAGATATTAGCATATGTGTTTGTTTGGCAATACTGACATCTTTATTTGACGAAGAAGGAACTTTCGATTGCGGGAAATCTTTTAACAACACGCTTATTACAAAATTGGAAATGAAAAGACGACTCGTATACATCTGTAAATTTGCAACAAATGCAAGGCCTTCCAGAGGAAATTTGAGGCAGGTTTTCAATTTTCTTAGTGGAGGAAGAACTGATTAA

Protein sequence

MWRNWLKGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSISDVLAENGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRKPLRPLWISQRSVIWLNEVPDHDSWDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDDEESWARGLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKGSTQVPPIEQMLSFDISDAELDVKSSDGNCGLSWLGLTKLAVGSSQYAVTACDVDCILNCDQETLSVCLPVASAYLHLPLVMSKMDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGKDISICVCLAILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQVFNFLSGGRTD*

Homology

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BLAST of CSPI02G00860.3 vs. ExPASy Swiss-Prot
Match: Q10181 (Uncharacterized protein C3F10.06c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC3F10.06c PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 6.3e-28
Identity = 111/429 (25.87%), Postives = 179/429 (41.72%), Query Frame = 0


Query: 9   GCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSISDVLAENG 68
           G  IVDSTRRGKR PD++SKTIPIW   LN+ + + L      +  L             
Sbjct: 98  GLIIVDSTRRGKRMPDALSKTIPIWIATLNKCVFERLRPHSFPNARLA------------ 157

Query: 69  KECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRKPLRPLWIS 128
                       LP ++ +TEK++I +RL+ +   L   G D+  LA+ L KP+RPLW++
Sbjct: 158 -----------FLPPFLPDTEKSSILQRLDGFVDSLMQSGIDLDALAAKLTKPIRPLWVT 217

Query: 129 QRSVIWLNEVPDHDSWDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDDEESWARGL 188
             S +   +  ++    FT ++LV AS+    VQ   + E  + Y+ GA DDEE W+ GL
Sbjct: 218 PASRLTSAQFEEY----FT-VVLVTASAQ---VQNGYSREHGFLYVQGAADDEEEWSHGL 277

Query: 189 TPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKGSTQVPPIE 248
           TP +FW++   +++   +   QK++ ++   R        ++P +S              
Sbjct: 278 TPEVFWQNTESILTCPEEQLEQKISLLLSSTR--------NSPTMS-------------- 337

Query: 249 QMLSFDISDAELDVKSSDGNCGLSWLGLTKLAVG--SSQYAVTACDVDCILNCDQETLSV 308
                              N  L+ L  T + VG  +  Y         +LN    TL+V
Sbjct: 338 -------------------NSSLTHLLPTPIFVGDVTGFYPPPENTSYFVLNLSNTTLNV 397

Query: 309 ----CLPVASAYLHLPLVMSKMDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGKDISI 368
               C P+ S     P+            N      + +  +      +V   N K+ + 
Sbjct: 398 KNELCFPIPSGKKGAPVFRKHFPSILQELNSLDPPFYERDAI-----FIVDEGNAKEAAS 449

Query: 369 CVCLAILTSLFDEEG---TFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQV 428
           C+ L IL   +D            S + + +TK  +++ LV I +  +   PSR  L  V
Sbjct: 458 CLALMILCLYYDLHMHLLAHPISLSASQSHLTKQTVRQFLVKITELHSKTNPSRAFLLAV 449

[Close]

BLAST of CSPI02G00860.3 vs. ExPASy Swiss-Prot
Match: P23796 (tRNA A64-2'-O-ribosylphosphate transferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RIT1 PE=1 SV=3)

HSP 1 Score: 102.4 bits (254), Expect = 1.3e-20
Identity = 115/446 (25.78%), Postives = 184/446 (41.26%), Query Frame = 0


Query: 9   GCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSISDVLAENG 68
           G  IVDSTRRGK+ PD++SKT+PIW  VLN                      + +L E  
Sbjct: 121 GIIIVDSTRRGKKIPDALSKTVPIWCAVLN----------------------TLMLQETE 180

Query: 69  KECAVGWDCSLHL-PLWVSETEKAAIEERLEEWTKQLEDCG-ADVATLASI-LRKPLRPL 128
           K  A+  D  L+L P  V ++E   I+ ++ E   +L+     D   L  + + K LRP+
Sbjct: 181 KNVAI--DKVLYLPPETVPKSEYDMIKRKIPELVAKLQKLNIIDSKKLNELFMGKLLRPI 240

Query: 129 WISQRSVI-------WLNEVPDHDSWD------FTPIILVNASSSTGVVQQRTTTEFSWN 188
           W+   S +       +  EV ++++W+        PIIL   S      Q      + + 
Sbjct: 241 WVHPGSSLLDHSVDYFTGEVQEYEAWETPEDQNIIPIILCTVSYQ---AQDGMDKRYGFT 300

Query: 189 YISGAGDDEESWARGLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQ 248
           Y+ GA DD E W+ GL  N+FW H   L  +               D+++  +    A +
Sbjct: 301 YVQGAADDHELWSFGLDSNMFWAHIEYLGDAS-----------YSDDQLHDYIMDLAAAK 360

Query: 249 ISVKSQKGSTQVPPIEQMLSFDISDAELDVKSSDGNCGLSWLGLT-------KLAVGSSQ 308
           +  +          I+   S D     +D  +++ + G    GLT       KL     +
Sbjct: 361 LRNQCY--------IQDKGSLDEVFGNIDKITNEISLGKVSSGLTINKNLKQKLKSEYGK 420

Query: 309 YAVTACDVDCILNCDQETLSVCLPVASAYLHLPLVMSKMDRFSLSRNLASAV-----DFA 368
             + +  V    + D E  S   P  S Y      +   D+ S S+ L S       +  
Sbjct: 421 VIIFSNSVTVAEDTDDEEESGTDPFISIY-----KLQSGDKKS-SKALRSTFPRIHGEIQ 480

Query: 369 KLNLNQG---KRLLVCCSNGKDISICVCLAILTSLFDEEGTFDCGKSFNNTLITKLEMKR 424
            L  N+    K +L+CC+ G D+SI V L+IL + + EE          + LI +  + +
Sbjct: 481 SLFTNRDEKIKPMLICCNTGTDMSIGVILSILCTKYTEEWMLTSELPDISKLIVRKHLTK 512

[Close]

BLAST of CSPI02G00860.3 vs. ExPASy TrEMBL
Match: A0A0A0LHV7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000910 PE=4 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 1.5e-250
Identity = 424/430 (98.60%), Postives = 428/430 (99.53%), Query Frame = 0


Query: 1   MWRNWLKGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSI 60
           MWRNWLKGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSI
Sbjct: 1   MWRNWLKGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSI 60

Query: 61  SDVLAENGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRK 120
           SDVLAENGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRK
Sbjct: 61  SDVLAENGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRK 120

Query: 121 PLRPLWISQRSVIWLNEVPDHDSWDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDD 180
           PLRPLWISQRSVIWLNEVPDHD WDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDD
Sbjct: 121 PLRPLWISQRSVIWLNEVPDHDFWDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDD 180

Query: 181 EESWARGLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKG 240
           EESWARGLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKG
Sbjct: 181 EESWARGLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKG 240

Query: 241 -STQVPPIEQMLSFDISDAELDVKSSDGNCGLSWLGLTKLAVGSSQYAVTACDVDCILNC 300
            STQVPPIEQMLSFDISDAELDVKSSDGNCGLSWLGLT+LAVGSSQYAVTACD+DCILNC
Sbjct: 241 SSTQVPPIEQMLSFDISDAELDVKSSDGNCGLSWLGLTRLAVGSSQYAVTACDIDCILNC 300

Query: 301 DQETLSVCLPVASAYLHLPLVMSKMDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGKD 360
           DQETLSVCLPVASAYLHLPLVMSK+DRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNG+D
Sbjct: 301 DQETLSVCLPVASAYLHLPLVMSKLDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGED 360

Query: 361 ISICVCLAILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQV 420
           ISICVCLAILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQV
Sbjct: 361 ISICVCLAILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQV 420

Query: 421 FNFLSGGRTD 430
           FNFLSGGRTD
Sbjct: 421 FNFLSGGRTD 430

[Close]

BLAST of CSPI02G00860.3 vs. ExPASy TrEMBL
Match: A0A1S3C8T8 (uncharacterized protein C3F10.06c isoform X2 OS=Cucumis melo OX=3656 GN=LOC103498092 PE=4 SV=1)

HSP 1 Score: 828.9 bits (2140), Expect = 9.5e-237
Identity = 400/430 (93.02%), Postives = 414/430 (96.28%), Query Frame = 0


Query: 1   MWRNWLKGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSI 60
           MWRNWLKGGC +VDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHL KIHGSS ELGEPS 
Sbjct: 1   MWRNWLKGGCIVVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLKKIHGSSSELGEPST 60

Query: 61  SDVLAENGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRK 120
           S VLAENGKEC VGWDCSLHLPLWVSETEKAAIEERLEEWTKQL+DCGADV TLASILRK
Sbjct: 61  SAVLAENGKECTVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLDDCGADVTTLASILRK 120

Query: 121 PLRPLWISQRSVIWLNEVPDHDSWDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDD 180
           PLRPLWISQRSVIWLNEVPDHDSWDFTPIILV AS STGVVQQRTTTEFSWNYISGAGDD
Sbjct: 121 PLRPLWISQRSVIWLNEVPDHDSWDFTPIILVTASFSTGVVQQRTTTEFSWNYISGAGDD 180

Query: 181 EESWARGLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKG 240
           EESWARGLTPNLFW HAHDLMSSGPD+CNQKVAEIVEKDRVYRALRGNDAPQISVKS+KG
Sbjct: 181 EESWARGLTPNLFWNHAHDLMSSGPDICNQKVAEIVEKDRVYRALRGNDAPQISVKSRKG 240

Query: 241 -STQVPPIEQMLSFDISDAELDVKSSDGNCGLSWLGLTKLAVGSSQYAVTACDVDCILNC 300
            STQVPPIEQMLSFDISDAELD KSSDGNCGLSWLG T+LAVGSSQ AVTA +VDCILNC
Sbjct: 241 SSTQVPPIEQMLSFDISDAELDAKSSDGNCGLSWLGSTRLAVGSSQNAVTASNVDCILNC 300

Query: 301 DQETLSVCLPVASAYLHLPLVMSKMDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGKD 360
           DQE+LSVCLPVASAYLHLP++MSK+DRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNG+D
Sbjct: 301 DQESLSVCLPVASAYLHLPMMMSKLDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGED 360

Query: 361 ISICVCLAILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQV 420
           ISICVCLAILTSLFD+EGTFDCGKSF+NT ITKLEM+RRLVYICKFATNARPSRGNLRQV
Sbjct: 361 ISICVCLAILTSLFDDEGTFDCGKSFDNTHITKLEMRRRLVYICKFATNARPSRGNLRQV 420

Query: 421 FNFLSGGRTD 430
           FNFLSGGRTD
Sbjct: 421 FNFLSGGRTD 430

[Close]

BLAST of CSPI02G00860.3 vs. ExPASy TrEMBL
Match: A0A1S3C9X8 (uncharacterized protein C3F10.06c isoform X1 OS=Cucumis melo OX=3656 GN=LOC103498092 PE=4 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 7.0e-232
Identity = 394/424 (92.92%), Postives = 408/424 (96.23%), Query Frame = 0


Query: 7   KGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSISDVLAE 66
           KGGC +VDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHL KIHGSS ELGEPS S VLAE
Sbjct: 97  KGGCIVVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLKKIHGSSSELGEPSTSAVLAE 156

Query: 67  NGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRKPLRPLW 126
           NGKEC VGWDCSLHLPLWVSETEKAAIEERLEEWTKQL+DCGADV TLASILRKPLRPLW
Sbjct: 157 NGKECTVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLDDCGADVTTLASILRKPLRPLW 216

Query: 127 ISQRSVIWLNEVPDHDSWDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDDEESWAR 186
           ISQRSVIWLNEVPDHDSWDFTPIILV AS STGVVQQRTTTEFSWNYISGAGDDEESWAR
Sbjct: 217 ISQRSVIWLNEVPDHDSWDFTPIILVTASFSTGVVQQRTTTEFSWNYISGAGDDEESWAR 276

Query: 187 GLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKG-STQVP 246
           GLTPNLFW HAHDLMSSGPD+CNQKVAEIVEKDRVYRALRGNDAPQISVKS+KG STQVP
Sbjct: 277 GLTPNLFWNHAHDLMSSGPDICNQKVAEIVEKDRVYRALRGNDAPQISVKSRKGSSTQVP 336

Query: 247 PIEQMLSFDISDAELDVKSSDGNCGLSWLGLTKLAVGSSQYAVTACDVDCILNCDQETLS 306
           PIEQMLSFDISDAELD KSSDGNCGLSWLG T+LAVGSSQ AVTA +VDCILNCDQE+LS
Sbjct: 337 PIEQMLSFDISDAELDAKSSDGNCGLSWLGSTRLAVGSSQNAVTASNVDCILNCDQESLS 396

Query: 307 VCLPVASAYLHLPLVMSKMDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGKDISICVC 366
           VCLPVASAYLHLP++MSK+DRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNG+DISICVC
Sbjct: 397 VCLPVASAYLHLPMMMSKLDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGEDISICVC 456

Query: 367 LAILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQVFNFLSG 426
           LAILTSLFD+EGTFDCGKSF+NT ITKLEM+RRLVYICKFATNARPSRGNLRQVFNFLSG
Sbjct: 457 LAILTSLFDDEGTFDCGKSFDNTHITKLEMRRRLVYICKFATNARPSRGNLRQVFNFLSG 516

Query: 427 GRTD 430
           GRTD
Sbjct: 517 GRTD 520

[Close]

BLAST of CSPI02G00860.3 vs. ExPASy TrEMBL
Match: A0A6J1G508 (uncharacterized protein C3F10.06c isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450792 PE=4 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.2e-220
Identity = 374/426 (87.79%), Postives = 398/426 (93.43%), Query Frame = 0


Query: 7   KGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSISDVLAE 66
           KGGC IVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLE GEPS SDVL E
Sbjct: 97  KGGCIIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLEQGEPSTSDVLTE 156

Query: 67  NGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRKPLRPLW 126
           NG+E AV WDCSLHLPLWVS+TEKAAIEERLEEWTKQL++CGAD+ATLASILRKPLRPLW
Sbjct: 157 NGRESAVNWDCSLHLPLWVSQTEKAAIEERLEEWTKQLDECGADIATLASILRKPLRPLW 216

Query: 127 ISQRSVIWLNEVPDHDSWDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDDEESWAR 186
           ISQRSVIWLNEVPDHDSWDFTPIILV+AS STGVVQQRTTTEFSWNYISGAGDDEESWAR
Sbjct: 217 ISQRSVIWLNEVPDHDSWDFTPIILVSASFSTGVVQQRTTTEFSWNYISGAGDDEESWAR 276

Query: 187 GLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQK---GSTQ 246
           GL+PNLFW HA+DLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQK    STQ
Sbjct: 277 GLSPNLFWNHAYDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKVGGSSTQ 336

Query: 247 VPPIEQMLSFDISDAELDVKSSDGNCGLSWLGLTKLAVGSSQYAVTACDVDCILNCDQET 306
           VP IEQMLS DISD ELD KSSDGNC LSWLG T LA+GSSQ+AVTACDVDCILNCDQE+
Sbjct: 337 VPSIEQMLSLDISDTELDAKSSDGNCALSWLGSTSLAIGSSQHAVTACDVDCILNCDQES 396

Query: 307 LSVCLPVASAYLHLPLVMSKMDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGKDISIC 366
           LSVCL +A AYLHLP+ MSK+DRFSLSR+L +AVDFAK NL++GKRLLVCCSNG+DIS+C
Sbjct: 397 LSVCLLIADAYLHLPMAMSKLDRFSLSRSLPAAVDFAKSNLSEGKRLLVCCSNGEDISVC 456

Query: 367 VCLAILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQVFNFL 426
           VCLAILTSLFD++GTFD GKSFN T ITK +M+RRLVYICKFATNARPSRGNLRQVFNFL
Sbjct: 457 VCLAILTSLFDDKGTFDGGKSFNKTRITKSDMRRRLVYICKFATNARPSRGNLRQVFNFL 516

Query: 427 SGGRTD 430
           SGGRTD
Sbjct: 517 SGGRTD 522

[Close]

BLAST of CSPI02G00860.3 vs. ExPASy TrEMBL
Match: A0A6J1KD61 (uncharacterized protein C3F10.06c isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493823 PE=4 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.3e-219
Identity = 371/425 (87.29%), Postives = 393/425 (92.47%), Query Frame = 0


Query: 7   KGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSISDVLAE 66
           KGGC IVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLE G+PS SDVL E
Sbjct: 97  KGGCIIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLEQGDPSTSDVLTE 156

Query: 67  NGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRKPLRPLW 126
           NG+E AV WDCSLHLPLWVS TEKAAIEERLEEWT+QL+DCGAD+ATLASILRKPLRPLW
Sbjct: 157 NGREAAVNWDCSLHLPLWVSRTEKAAIEERLEEWTEQLDDCGADIATLASILRKPLRPLW 216

Query: 127 ISQRSVIWLNEVPDHDSWDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDDEESWAR 186
           ISQRSVIWLNEVPDHDSWDFTPIILV+AS STGV QQRTTTEFSWNYISGAGDDEESWAR
Sbjct: 217 ISQRSVIWLNEVPDHDSWDFTPIILVSASFSTGVAQQRTTTEFSWNYISGAGDDEESWAR 276

Query: 187 GLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQK--GSTQV 246
           GL+PNLFW HAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQK   STQV
Sbjct: 277 GLSPNLFWNHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKVGSSTQV 336

Query: 247 PPIEQMLSFDISDAELDVKSSDGNCGLSWLGLTKLAVGSSQYAVTACDVDCILNCDQETL 306
           P IEQM S DISD ELD KSSDGNC LSWLG T LAVGSSQ+AVTACDVDCILNCD+E+L
Sbjct: 337 PSIEQMSSLDISDTELDAKSSDGNCALSWLGSTSLAVGSSQHAVTACDVDCILNCDKESL 396

Query: 307 SVCLPVASAYLHLPLVMSKMDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGKDISICV 366
           SVCLP+A AYLHLP+ MSK+DRFSLSR+L  AVDFAK NL++GKRLLVCCSNG+DIS+CV
Sbjct: 397 SVCLPIADAYLHLPMAMSKLDRFSLSRSLPVAVDFAKSNLSEGKRLLVCCSNGEDISVCV 456

Query: 367 CLAILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQVFNFLS 426
           CLAILTSLFD++GTFD GKSFN T ITK +M+RRLVYICKF TNARPSRGNLRQVFNFL 
Sbjct: 457 CLAILTSLFDDKGTFDGGKSFNKTCITKSDMRRRLVYICKFVTNARPSRGNLRQVFNFLR 516

Query: 427 GGRTD 430
           GGRTD
Sbjct: 517 GGRTD 521

[Close]

BLAST of CSPI02G00860.3 vs. NCBI nr
Match: XP_011648633.1 (tRNA A64-2'-O-ribosylphosphate transferase isoform X2 [Cucumis sativus])

HSP 1 Score: 874.8 bits (2259), Expect = 3.1e-250
Identity = 424/430 (98.60%), Postives = 428/430 (99.53%), Query Frame = 0


Query: 1   MWRNWLKGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSI 60
           MWRNWLKGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSI
Sbjct: 1   MWRNWLKGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSI 60

Query: 61  SDVLAENGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRK 120
           SDVLAENGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRK
Sbjct: 61  SDVLAENGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRK 120

Query: 121 PLRPLWISQRSVIWLNEVPDHDSWDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDD 180
           PLRPLWISQRSVIWLNEVPDHD WDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDD
Sbjct: 121 PLRPLWISQRSVIWLNEVPDHDFWDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDD 180

Query: 181 EESWARGLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKG 240
           EESWARGLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKG
Sbjct: 181 EESWARGLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKG 240

Query: 241 -STQVPPIEQMLSFDISDAELDVKSSDGNCGLSWLGLTKLAVGSSQYAVTACDVDCILNC 300
            STQVPPIEQMLSFDISDAELDVKSSDGNCGLSWLGLT+LAVGSSQYAVTACD+DCILNC
Sbjct: 241 SSTQVPPIEQMLSFDISDAELDVKSSDGNCGLSWLGLTRLAVGSSQYAVTACDIDCILNC 300

Query: 301 DQETLSVCLPVASAYLHLPLVMSKMDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGKD 360
           DQETLSVCLPVASAYLHLPLVMSK+DRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNG+D
Sbjct: 301 DQETLSVCLPVASAYLHLPLVMSKLDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGED 360

Query: 361 ISICVCLAILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQV 420
           ISICVCLAILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQV
Sbjct: 361 ISICVCLAILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQV 420

Query: 421 FNFLSGGRTD 430
           FNFLSGGRTD
Sbjct: 421 FNFLSGGRTD 430

[Close]

BLAST of CSPI02G00860.3 vs. NCBI nr
Match: XP_004141018.1 (tRNA A64-2'-O-ribosylphosphate transferase isoform X1 [Cucumis sativus] >XP_031736416.1 tRNA A64-2'-O-ribosylphosphate transferase isoform X1 [Cucumis sativus] >KGN60599.2 hypothetical protein Csa_019352 [Cucumis sativus])

HSP 1 Score: 858.6 bits (2217), Expect = 2.3e-245
Identity = 418/424 (98.58%), Postives = 422/424 (99.53%), Query Frame = 0


Query: 7   KGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSISDVLAE 66
           KGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSISDVLAE
Sbjct: 97  KGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSISDVLAE 156

Query: 67  NGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRKPLRPLW 126
           NGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRKPLRPLW
Sbjct: 157 NGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRKPLRPLW 216

Query: 127 ISQRSVIWLNEVPDHDSWDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDDEESWAR 186
           ISQRSVIWLNEVPDHD WDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDDEESWAR
Sbjct: 217 ISQRSVIWLNEVPDHDFWDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDDEESWAR 276

Query: 187 GLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKG-STQVP 246
           GLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKG STQVP
Sbjct: 277 GLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKGSSTQVP 336

Query: 247 PIEQMLSFDISDAELDVKSSDGNCGLSWLGLTKLAVGSSQYAVTACDVDCILNCDQETLS 306
           PIEQMLSFDISDAELDVKSSDGNCGLSWLGLT+LAVGSSQYAVTACD+DCILNCDQETLS
Sbjct: 337 PIEQMLSFDISDAELDVKSSDGNCGLSWLGLTRLAVGSSQYAVTACDIDCILNCDQETLS 396

Query: 307 VCLPVASAYLHLPLVMSKMDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGKDISICVC 366
           VCLPVASAYLHLPLVMSK+DRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNG+DISICVC
Sbjct: 397 VCLPVASAYLHLPLVMSKLDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGEDISICVC 456

Query: 367 LAILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQVFNFLSG 426
           LAILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQVFNFLSG
Sbjct: 457 LAILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQVFNFLSG 516

Query: 427 GRTD 430
           GRTD
Sbjct: 517 GRTD 520

[Close]

BLAST of CSPI02G00860.3 vs. NCBI nr
Match: XP_008458800.1 (PREDICTED: uncharacterized protein C3F10.06c isoform X2 [Cucumis melo])

HSP 1 Score: 828.9 bits (2140), Expect = 2.0e-236
Identity = 400/430 (93.02%), Postives = 414/430 (96.28%), Query Frame = 0


Query: 1   MWRNWLKGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSI 60
           MWRNWLKGGC +VDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHL KIHGSS ELGEPS 
Sbjct: 1   MWRNWLKGGCIVVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLKKIHGSSSELGEPST 60

Query: 61  SDVLAENGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRK 120
           S VLAENGKEC VGWDCSLHLPLWVSETEKAAIEERLEEWTKQL+DCGADV TLASILRK
Sbjct: 61  SAVLAENGKECTVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLDDCGADVTTLASILRK 120

Query: 121 PLRPLWISQRSVIWLNEVPDHDSWDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDD 180
           PLRPLWISQRSVIWLNEVPDHDSWDFTPIILV AS STGVVQQRTTTEFSWNYISGAGDD
Sbjct: 121 PLRPLWISQRSVIWLNEVPDHDSWDFTPIILVTASFSTGVVQQRTTTEFSWNYISGAGDD 180

Query: 181 EESWARGLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKG 240
           EESWARGLTPNLFW HAHDLMSSGPD+CNQKVAEIVEKDRVYRALRGNDAPQISVKS+KG
Sbjct: 181 EESWARGLTPNLFWNHAHDLMSSGPDICNQKVAEIVEKDRVYRALRGNDAPQISVKSRKG 240

Query: 241 -STQVPPIEQMLSFDISDAELDVKSSDGNCGLSWLGLTKLAVGSSQYAVTACDVDCILNC 300
            STQVPPIEQMLSFDISDAELD KSSDGNCGLSWLG T+LAVGSSQ AVTA +VDCILNC
Sbjct: 241 SSTQVPPIEQMLSFDISDAELDAKSSDGNCGLSWLGSTRLAVGSSQNAVTASNVDCILNC 300

Query: 301 DQETLSVCLPVASAYLHLPLVMSKMDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGKD 360
           DQE+LSVCLPVASAYLHLP++MSK+DRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNG+D
Sbjct: 301 DQESLSVCLPVASAYLHLPMMMSKLDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGED 360

Query: 361 ISICVCLAILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQV 420
           ISICVCLAILTSLFD+EGTFDCGKSF+NT ITKLEM+RRLVYICKFATNARPSRGNLRQV
Sbjct: 361 ISICVCLAILTSLFDDEGTFDCGKSFDNTHITKLEMRRRLVYICKFATNARPSRGNLRQV 420

Query: 421 FNFLSGGRTD 430
           FNFLSGGRTD
Sbjct: 421 FNFLSGGRTD 430

[Close]

BLAST of CSPI02G00860.3 vs. NCBI nr
Match: XP_008458793.1 (PREDICTED: uncharacterized protein C3F10.06c isoform X1 [Cucumis melo] >XP_016902539.1 PREDICTED: uncharacterized protein C3F10.06c isoform X1 [Cucumis melo])

HSP 1 Score: 812.8 bits (2098), Expect = 1.4e-231
Identity = 394/424 (92.92%), Postives = 408/424 (96.23%), Query Frame = 0


Query: 7   KGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSISDVLAE 66
           KGGC +VDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHL KIHGSS ELGEPS S VLAE
Sbjct: 97  KGGCIVVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLKKIHGSSSELGEPSTSAVLAE 156

Query: 67  NGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRKPLRPLW 126
           NGKEC VGWDCSLHLPLWVSETEKAAIEERLEEWTKQL+DCGADV TLASILRKPLRPLW
Sbjct: 157 NGKECTVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLDDCGADVTTLASILRKPLRPLW 216

Query: 127 ISQRSVIWLNEVPDHDSWDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDDEESWAR 186
           ISQRSVIWLNEVPDHDSWDFTPIILV AS STGVVQQRTTTEFSWNYISGAGDDEESWAR
Sbjct: 217 ISQRSVIWLNEVPDHDSWDFTPIILVTASFSTGVVQQRTTTEFSWNYISGAGDDEESWAR 276

Query: 187 GLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKG-STQVP 246
           GLTPNLFW HAHDLMSSGPD+CNQKVAEIVEKDRVYRALRGNDAPQISVKS+KG STQVP
Sbjct: 277 GLTPNLFWNHAHDLMSSGPDICNQKVAEIVEKDRVYRALRGNDAPQISVKSRKGSSTQVP 336

Query: 247 PIEQMLSFDISDAELDVKSSDGNCGLSWLGLTKLAVGSSQYAVTACDVDCILNCDQETLS 306
           PIEQMLSFDISDAELD KSSDGNCGLSWLG T+LAVGSSQ AVTA +VDCILNCDQE+LS
Sbjct: 337 PIEQMLSFDISDAELDAKSSDGNCGLSWLGSTRLAVGSSQNAVTASNVDCILNCDQESLS 396

Query: 307 VCLPVASAYLHLPLVMSKMDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGKDISICVC 366
           VCLPVASAYLHLP++MSK+DRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNG+DISICVC
Sbjct: 397 VCLPVASAYLHLPMMMSKLDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGEDISICVC 456

Query: 367 LAILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQVFNFLSG 426
           LAILTSLFD+EGTFDCGKSF+NT ITKLEM+RRLVYICKFATNARPSRGNLRQVFNFLSG
Sbjct: 457 LAILTSLFDDEGTFDCGKSFDNTHITKLEMRRRLVYICKFATNARPSRGNLRQVFNFLSG 516

Query: 427 GRTD 430
           GRTD
Sbjct: 517 GRTD 520

[Close]

BLAST of CSPI02G00860.3 vs. NCBI nr
Match: KAG7030455.1 (SPAC3F10.06c [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 781.6 bits (2017), Expect = 3.6e-222
Identity = 377/426 (88.50%), Postives = 399/426 (93.66%), Query Frame = 0


Query: 7   KGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSISDVLAE 66
           KGGC IVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLE GEPS SDVL E
Sbjct: 97  KGGCIIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLEQGEPSTSDVLTE 156

Query: 67  NGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRKPLRPLW 126
           NG+E AV WDCSLHLPLWVS+TEKAAIEERLEEW+KQL+DCGAD+ATLASILRKPLRPLW
Sbjct: 157 NGREFAVNWDCSLHLPLWVSQTEKAAIEERLEEWSKQLDDCGADIATLASILRKPLRPLW 216

Query: 127 ISQRSVIWLNEVPDHDSWDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDDEESWAR 186
           ISQRSVIWLNEVPDHDSWDFTPIILV+AS STGVVQQRTTTEFSWNYISGAGDDEESWAR
Sbjct: 217 ISQRSVIWLNEVPDHDSWDFTPIILVSASFSTGVVQQRTTTEFSWNYISGAGDDEESWAR 276

Query: 187 GLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQK---GSTQ 246
           GL+PNLFW HAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQK    STQ
Sbjct: 277 GLSPNLFWNHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKVGGSSTQ 336

Query: 247 VPPIEQMLSFDISDAELDVKSSDGNCGLSWLGLTKLAVGSSQYAVTACDVDCILNCDQET 306
           VP IEQMLS DISD ELD KSSDGNC LSWLG T LAVGSSQ+AVTACDVDCILNCDQE+
Sbjct: 337 VPSIEQMLSLDISDTELDAKSSDGNCALSWLGSTSLAVGSSQHAVTACDVDCILNCDQES 396

Query: 307 LSVCLPVASAYLHLPLVMSKMDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGKDISIC 366
           LSVCLP+A AYLHLP+ MSK+DRFSLSR+L +AVDFAK NL++GKRLLVCCSNG+DIS+C
Sbjct: 397 LSVCLPIADAYLHLPMAMSKLDRFSLSRSLPAAVDFAKSNLSEGKRLLVCCSNGEDISVC 456

Query: 367 VCLAILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQVFNFL 426
           VCLAILTSLFD++GTFD GKSFN T ITK +M+RRLVYICKFATNARPSRGNLRQVFNFL
Sbjct: 457 VCLAILTSLFDDKGTFDGGKSFNKTRITKSDMRRRLVYICKFATNARPSRGNLRQVFNFL 516

Query: 427 SGGRTD 430
           SGGRTD
Sbjct: 517 SGGRTD 522

[Close]

BLAST of CSPI02G00860.3 vs. TAIR 10
Match: AT2G40570.1 (initiator tRNA phosphoribosyl transferase family protein )

HSP 1 Score: 530.0 bits (1364), Expect = 1.7e-150
Identity = 257/421 (61.05%), Postives = 321/421 (76.25%), Query Frame = 0


Query: 7   KGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSISDVLAE 66
           KGGC I+DSTR+GKRFPDSMSKTIP+W+CV+NRSI  H N++   +++ G  S  D   +
Sbjct: 104 KGGCIIIDSTRKGKRFPDSMSKTIPMWSCVVNRSIFNHWNRL--CNIDAGLTSDDD--GD 163

Query: 67  NGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRKPLRPLW 126
           N ++    WDCSLHLPLWVS TE+A+IE RL+EWT++L++ GAD+A+LAS LRKPLRPLW
Sbjct: 164 NIRKLLDKWDCSLHLPLWVSNTERASIEARLDEWTRELDESGADIASLASCLRKPLRPLW 223

Query: 127 ISQRSVIWLNEVPDHDSWDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDDEESWAR 186
           +SQ++VIWLNEVP+HDSWDFTP+ILV+AS+S G +Q RT++EFSWNYI GAGDDEESWAR
Sbjct: 224 VSQKTVIWLNEVPEHDSWDFTPLILVSASAS-GELQNRTSSEFSWNYIPGAGDDEESWAR 283

Query: 187 GLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKGSTQVPP 246
           GL+PN+FW H  DL+ SGPD+CNQKVAEIVE DRVYRA RG +APQ+ VK  K +  V  
Sbjct: 284 GLSPNVFWTHVDDLIHSGPDLCNQKVAEIVENDRVYRAHRGQEAPQVVVKCSKSNGGV-- 343

Query: 247 IEQMLSFDISDAELDVKSSDGNCGLSWLGLTKLAVGSSQYAVTACDVDCILNCDQETLSV 306
                S +I      +   D    + WL  T LAVG+SQ A     +DCILNCDQ  +SV
Sbjct: 344 -NHAKSDEILCLSAQIPKVDEERLVFWLSSTNLAVGASQVACKETSIDCILNCDQNPISV 403

Query: 307 CLPVASAYLHLPLVMSKMDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGKDISICVCL 366
            +     +LHLP+  SK DRFS+SRNL  AV+FAKL ++ GK++LVCC +G+DISICVCL
Sbjct: 404 PVSYLEEHLHLPMKGSKFDRFSISRNLPPAVNFAKLKMSSGKKVLVCCQDGEDISICVCL 463

Query: 367 AILTSLFDEEGTFDCGKSFNNTLITKLEMKRRLVYICKFATNARPSRGNLRQVFNFLSGG 426
           AIL SLF+EEG FD GKSF    ITK++M+R L++ICK+A NARPSRGNL+QVF FLS  
Sbjct: 464 AILMSLFNEEGAFDGGKSFEEKSITKMDMRRMLIFICKYAVNARPSRGNLKQVFGFLSSH 516

Query: 427 R 428
           R
Sbjct: 524 R 516

[Close]

BLAST of CSPI02G00860.3 vs. TAIR 10
Match: AT2G40570.2 (initiator tRNA phosphoribosyl transferase family protein )

HSP 1 Score: 431.8 bits (1109), Expect = 6.5e-121
Identity = 210/358 (58.66%), Postives = 267/358 (74.58%), Query Frame = 0


Query: 7   KGGCFIVDSTRRGKRFPDSMSKTIPIWTCVLNRSISKHLNKIHGSSLELGEPSISDVLAE 66
           KGGC I+DSTR+GKRFPDSMSKTIP+W+CV+NRSI  H N++   +++ G  S  D   +
Sbjct: 104 KGGCIIIDSTRKGKRFPDSMSKTIPMWSCVVNRSIFNHWNRL--CNIDAGLTSDDD--GD 163

Query: 67  NGKECAVGWDCSLHLPLWVSETEKAAIEERLEEWTKQLEDCGADVATLASILRKPLRPLW 126
           N ++    WDCSLHLPLWVS TE+A+IE RL+EWT++L++ GAD+A+LAS LRKPLRPLW
Sbjct: 164 NIRKLLDKWDCSLHLPLWVSNTERASIEARLDEWTRELDESGADIASLASCLRKPLRPLW 223

Query: 127 ISQRSVIWLNEVPDHDSWDFTPIILVNASSSTGVVQQRTTTEFSWNYISGAGDDEESWAR 186
           +SQ++VIWLNEVP+HDSWDFTP+ILV+AS+S G +Q RT++EFSWNYI GAGDDEESWAR
Sbjct: 224 VSQKTVIWLNEVPEHDSWDFTPLILVSASAS-GELQNRTSSEFSWNYIPGAGDDEESWAR 283

Query: 187 GLTPNLFWKHAHDLMSSGPDVCNQKVAEIVEKDRVYRALRGNDAPQISVKSQKGSTQVPP 246
           GL+PN+FW H  DL+ SGPD+CNQKVAEIVE DRVYRA RG +APQ+ VK  K +  V  
Sbjct: 284 GLSPNVFWTHVDDLIHSGPDLCNQKVAEIVENDRVYRAHRGQEAPQVVVKCSKSNGGV-- 343

Query: 247 IEQMLSFDISDAELDVKSSDGNCGLSWLGLTKLAVGSSQYAVTACDVDCILNCDQETLSV 306
                S +I      +   D    + WL  T LAVG+SQ A     +DCILNCDQ  +SV
Sbjct: 344 -NHAKSDEILCLSAQIPKVDEERLVFWLSSTNLAVGASQVACKETSIDCILNCDQNPISV 403

Query: 307 CLPVASAYLHLPLVMSKMDRFSLSRNLASAVDFAKLNLNQGKRLLVCCSNGKDISICV 365
            +     +LHLP+  SK DRFS+SRNL  AV+FAKL ++ GK++LVCC +GK   +C+
Sbjct: 404 PVSYLEEHLHLPMKGSKFDRFSISRNLPPAVNFAKLKMSSGKKVLVCCQDGKYQYMCL 453

The following BLAST results are available for this feature:

BLAST of CSPI02G00860.3 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q10181	6.3e-28	25.87	Uncharacterized protein C3F10.06c OS=Schizosaccharomyces pombe (strain 972 / ATC...	[more]
P23796	1.3e-20	25.78	tRNA A64-2'-O-ribosylphosphate transferase OS=Saccharomyces cerevisiae (strain A...	[more]

BLAST of CSPI02G00860.3 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LHV7	1.5e-250	98.60	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G000910 PE=4 SV=1	[more]
A0A1S3C8T8	9.5e-237	93.02	uncharacterized protein C3F10.06c isoform X2 OS=Cucumis melo OX=3656 GN=LOC10349...	[more]
A0A1S3C9X8	7.0e-232	92.92	uncharacterized protein C3F10.06c isoform X1 OS=Cucumis melo OX=3656 GN=LOC10349...	[more]
A0A6J1G508	1.2e-220	87.79	uncharacterized protein C3F10.06c isoform X1 OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A6J1KD61	2.3e-219	87.29	uncharacterized protein C3F10.06c isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]

BLAST of CSPI02G00860.3 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011648633.1	3.1e-250	98.60	tRNA A64-2'-O-ribosylphosphate transferase isoform X2 [Cucumis sativus]	[more]
XP_004141018.1	2.3e-245	98.58	tRNA A64-2'-O-ribosylphosphate transferase isoform X1 [Cucumis sativus] >XP_0317...	[more]
XP_008458800.1	2.0e-236	93.02	PREDICTED: uncharacterized protein C3F10.06c isoform X2 [Cucumis melo]	[more]
XP_008458793.1	1.4e-231	92.92	PREDICTED: uncharacterized protein C3F10.06c isoform X1 [Cucumis melo] >XP_01690...	[more]
KAG7030455.1	3.6e-222	88.50	SPAC3F10.06c [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CSPI02G00860.3 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G40570.1	1.7e-150	61.05	initiator tRNA phosphoribosyl transferase family protein	[more]
AT2G40570.2	6.5e-121	58.66	initiator tRNA phosphoribosyl transferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (PI 183967) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 90..110
IPR033421	Rit1, DUSP-like domain	PFAM	PF04179	Init_tRNA_PT	coord: 314..423 e-value: 3.9E-26 score: 91.5
IPR007306	tRNA A64-2'-O-ribosylphosphate transferase	PIRSF	PIRSF007747	RIT1	coord: 5..429 e-value: 1.1E-105 score: 352.5
IPR007306	tRNA A64-2'-O-ribosylphosphate transferase	PANTHER	PTHR31811	TRNA A64-2'-O-RIBOSYLPHOSPHATE TRANSFERASE	coord: 7..424
IPR033449	Rit1, N-terminal domain	PFAM	PF17184	Rit1_C	coord: 7..217 e-value: 3.5E-56 score: 190.6
IPR029021	Protein-tyrosine phosphatase-like	GENE3D	3.90.190.10	Protein tyrosine phosphatase superfamily	coord: 273..424 e-value: 4.5E-9 score: 38.3
IPR029021	Protein-tyrosine phosphatase-like	SUPERFAMILY	52799	(Phosphotyrosine protein) phosphatases II	coord: 290..372

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
CSPI02G00860	CSPI02G00860	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI02G00860.3.utr5p1	CSPI02G00860.3.utr5p1	five_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI02G00860.3.cds1	CSPI02G00860.3.cds1	CDS
CSPI02G00860.3.cds2	CSPI02G00860.3.cds2	CDS
CSPI02G00860.3.cds3	CSPI02G00860.3.cds3	CDS
CSPI02G00860.3.cds4	CSPI02G00860.3.cds4	CDS
CSPI02G00860.3.cds5	CSPI02G00860.3.cds5	CDS
CSPI02G00860.3.cds6	CSPI02G00860.3.cds6	CDS
CSPI02G00860.3.cds7	CSPI02G00860.3.cds7	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
CSPI02G00860.3.utr3p1	CSPI02G00860.3.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
CSPI02G00860.3	CSPI02G00860.3-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019988	charged-tRNA amino acid modification
molecular_function	GO:0043399	tRNA A64-2'-O-ribosylphosphate transferase activity