Bhi12M002205 (mRNA) Wax gourd (B227) v1

Overview
NameBhi12M002205
TypemRNA
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionAgglutinin domain-containing protein
Locationchr12: 78293673 .. 78295376 (-)
Sequence length1704
RNA-Seq ExpressionBhi12M002205
SyntenyBhi12M002205
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACACCTCGAGAAGAAGAAGAAAAAACTCTCTCATTCCATCTATAAAATATAATGGGTCTCCCTAAGTACGCTGCATTCAAAAGCGAAAAAGCTGGGAAGTACCTTCAATACCTTCATGAAGGTCCAATTAAAGGTAACCTACAATACTCTTCTGATAACGTTGCGAACATGTTCTCAAAGTTCACATTGGAGCCATCCAAGACGAGTAATTATGTAAACATAAGATGTTGCTACAACAACAAGTTCTGGGCTTCTTCCAACGACGACAATCACATGATCACTCCCTCCCTTAGCAAGCCCGCCGAAAACGAATTCTTATCACCATGCACATTGTTTAGGATTGTCAAAGCTCCGGCAGAAGGAGCTTACTATTTATTGGATGTGTTGCGTCAATCGTATGTCTGTCGATGTACCACCAGCAAAATTCATGAGAATTGCCTCACAACTAGATACGGCAACCAAGATCATTACTACGACAGATCTCACATATTAATCGACTTCGAAACCATCGTCATGCTCCCCAAACATGTGGCATTTAAATGCGACATAGAAAAGTATCTGTGCAACTATTGGTACGAGAATCATGAATATCTCAAATTCGATTCCGATGACATTGGATTCTCGAAGGTAGGCCACCAAGTGTTCAACGTGGGTGATGGAAGCATTCGAATCAAATGTGACCATTCGAATAAGTTTTGGGTGCGTCAACCAAACAACTGGATCCTCGCGAACTCAACCGACACAACCACTAACAACAAAGATACCTTGTTCTGGCCGGTTAAGGTTTCTAAAAACGTCGTGGCACTTCGCAACCTCGGCAATAACAGTTTCTGCAAGAGATTCACTCATGAGAATAAGGAGCATTGTCTCAGCGCTCATTCAAATACCATTTCGAGGGAGGCACAACTGGAGATCGTTGAGCCTATTATTTCAAGGGAAATCTACAATTGTAAGTATCGAACTATGGATGCTAGAGTGTATGACGAAAAGGTACTAAATATGGCAACCGGGGAAGCTACCAACGGCGCCACGAAAGAGACGAGTATGGCAGTAAGTTTGAAGTACGTAGTAGAAACATCTAAATGTTGGGAATCTTCGGTCACCGCGGGTTGGGGTGTGACGACATCTATCAAAGCTGGCATTCCTGAAATTGCAGAAGCTGGAATCGAAATCAACTATAATGAAGAGAAGAGTCACACGTGGGGAGAAACTATTACAGAAATGAAAGAAGTGATGGTAACATATACTGTCAGTGTACCAGCAAAGACAAGGATGAAAGTCACTCTTCTTGGAACCAAGGCCAAATGCGATGTCCCGTTTTCGTACACACGACGAGATGTTCAAAGGGATGGAAAACAAGTTATTACAGACTGTGATGATGGCCTTTATGTTGGAGTCAATTCCTACAAATTTGATTATCAAAACAAGCCTTTGCCTCTCTGATGAGCACCATATGATCTTAATATTAGACCTTTTCCTACAATGATCATCCTCTCTACTCTCTTCTCTCTCTATCATGGAATATACAAGTGTTATGTCTTGTGTGTGTATCTGTGCTTTATCTCTTGTTTTGGATAAAGCTTGAAATAAATGTCTAAATTAATACTATAATATAGTAAAATACAGCCTTTTATTTTGAAGCTTTTTATAAAACAACAACGTAATGCTTAACTGGCACCACAAACACCGCATAAAGTAAAGGA

mRNA sequence

CACACCTCGAGAAGAAGAAGAAAAAACTCTCTCATTCCATCTATAAAATATAATGGGTCTCCCTAAGTACGCTGCATTCAAAAGCGAAAAAGCTGGGAAGTACCTTCAATACCTTCATGAAGGTCCAATTAAAGGTAACCTACAATACTCTTCTGATAACGTTGCGAACATGTTCTCAAAGTTCACATTGGAGCCATCCAAGACGAGTAATTATGTAAACATAAGATGTTGCTACAACAACAAGTTCTGGGCTTCTTCCAACGACGACAATCACATGATCACTCCCTCCCTTAGCAAGCCCGCCGAAAACGAATTCTTATCACCATGCACATTGTTTAGGATTGTCAAAGCTCCGGCAGAAGGAGCTTACTATTTATTGGATGTGTTGCGTCAATCGTATGTCTGTCGATGTACCACCAGCAAAATTCATGAGAATTGCCTCACAACTAGATACGGCAACCAAGATCATTACTACGACAGATCTCACATATTAATCGACTTCGAAACCATCGTCATGCTCCCCAAACATGTGGCATTTAAATGCGACATAGAAAAGTATCTGTGCAACTATTGGTACGAGAATCATGAATATCTCAAATTCGATTCCGATGACATTGGATTCTCGAAGGTAGGCCACCAAGTGTTCAACGTGGGTGATGGAAGCATTCGAATCAAATGTGACCATTCGAATAAGTTTTGGGTGCGTCAACCAAACAACTGGATCCTCGCGAACTCAACCGACACAACCACTAACAACAAAGATACCTTGTTCTGGCCGGTTAAGGTTTCTAAAAACGTCGTGGCACTTCGCAACCTCGGCAATAACAGTTTCTGCAAGAGATTCACTCATGAGAATAAGGAGCATTGTCTCAGCGCTCATTCAAATACCATTTCGAGGGAGGCACAACTGGAGATCGTTGAGCCTATTATTTCAAGGGAAATCTACAATTGTAAGTATCGAACTATGGATGCTAGAGTGTATGACGAAAAGGTACTAAATATGGCAACCGGGGAAGCTACCAACGGCGCCACGAAAGAGACGAGTATGGCAGTAAGTTTGAAGTACGTAGTAGAAACATCTAAATGTTGGGAATCTTCGGTCACCGCGGGTTGGGGTGTGACGACATCTATCAAAGCTGGCATTCCTGAAATTGCAGAAGCTGGAATCGAAATCAACTATAATGAAGAGAAGAGTCACACGTGGGGAGAAACTATTACAGAAATGAAAGAAGTGATGGTAACATATACTGTCAGTGTACCAGCAAAGACAAGGATGAAAGTCACTCTTCTTGGAACCAAGGCCAAATGCGATGTCCCGTTTTCGTACACACGACGAGATGTTCAAAGGGATGGAAAACAAGTTATTACAGACTGTGATGATGGCCTTTATGTTGGAGTCAATTCCTACAAATTTGATTATCAAAACAAGCCTTTGCCTCTCTGATGAGCACCATATGATCTTAATATTAGACCTTTTCCTACAATGATCATCCTCTCTACTCTCTTCTCTCTCTATCATGGAATATACAAGTGTTATGTCTTGTGTGTGTATCTGTGCTTTATCTCTTGTTTTGGATAAAGCTTGAAATAAATGTCTAAATTAATACTATAATATAGTAAAATACAGCCTTTTATTTTGAAGCTTTTTATAAAACAACAACGTAATGCTTAACTGGCACCACAAACACCGCATAAAGTAAAGGA

Coding sequence (CDS)

ATGGGTCTCCCTAAGTACGCTGCATTCAAAAGCGAAAAAGCTGGGAAGTACCTTCAATACCTTCATGAAGGTCCAATTAAAGGTAACCTACAATACTCTTCTGATAACGTTGCGAACATGTTCTCAAAGTTCACATTGGAGCCATCCAAGACGAGTAATTATGTAAACATAAGATGTTGCTACAACAACAAGTTCTGGGCTTCTTCCAACGACGACAATCACATGATCACTCCCTCCCTTAGCAAGCCCGCCGAAAACGAATTCTTATCACCATGCACATTGTTTAGGATTGTCAAAGCTCCGGCAGAAGGAGCTTACTATTTATTGGATGTGTTGCGTCAATCGTATGTCTGTCGATGTACCACCAGCAAAATTCATGAGAATTGCCTCACAACTAGATACGGCAACCAAGATCATTACTACGACAGATCTCACATATTAATCGACTTCGAAACCATCGTCATGCTCCCCAAACATGTGGCATTTAAATGCGACATAGAAAAGTATCTGTGCAACTATTGGTACGAGAATCATGAATATCTCAAATTCGATTCCGATGACATTGGATTCTCGAAGGTAGGCCACCAAGTGTTCAACGTGGGTGATGGAAGCATTCGAATCAAATGTGACCATTCGAATAAGTTTTGGGTGCGTCAACCAAACAACTGGATCCTCGCGAACTCAACCGACACAACCACTAACAACAAAGATACCTTGTTCTGGCCGGTTAAGGTTTCTAAAAACGTCGTGGCACTTCGCAACCTCGGCAATAACAGTTTCTGCAAGAGATTCACTCATGAGAATAAGGAGCATTGTCTCAGCGCTCATTCAAATACCATTTCGAGGGAGGCACAACTGGAGATCGTTGAGCCTATTATTTCAAGGGAAATCTACAATTGTAAGTATCGAACTATGGATGCTAGAGTGTATGACGAAAAGGTACTAAATATGGCAACCGGGGAAGCTACCAACGGCGCCACGAAAGAGACGAGTATGGCAGTAAGTTTGAAGTACGTAGTAGAAACATCTAAATGTTGGGAATCTTCGGTCACCGCGGGTTGGGGTGTGACGACATCTATCAAAGCTGGCATTCCTGAAATTGCAGAAGCTGGAATCGAAATCAACTATAATGAAGAGAAGAGTCACACGTGGGGAGAAACTATTACAGAAATGAAAGAAGTGATGGTAACATATACTGTCAGTGTACCAGCAAAGACAAGGATGAAAGTCACTCTTCTTGGAACCAAGGCCAAATGCGATGTCCCGTTTTCGTACACACGACGAGATGTTCAAAGGGATGGAAAACAAGTTATTACAGACTGTGATGATGGCCTTTATGTTGGAGTCAATTCCTACAAATTTGATTATCAAAACAAGCCTTTGCCTCTCTGA

Protein sequence

MGLPKYAAFKSEKAGKYLQYLHEGPIKGNLQYSSDNVANMFSKFTLEPSKTSNYVNIRCCYNNKFWASSNDDNHMITPSLSKPAENEFLSPCTLFRIVKAPAEGAYYLLDVLRQSYVCRCTTSKIHENCLTTRYGNQDHYYDRSHILIDFETIVMLPKHVAFKCDIEKYLCNYWYENHEYLKFDSDDIGFSKVGHQVFNVGDGSIRIKCDHSNKFWVRQPNNWILANSTDTTTNNKDTLFWPVKVSKNVVALRNLGNNSFCKRFTHENKEHCLSAHSNTISREAQLEIVEPIISREIYNCKYRTMDARVYDEKVLNMATGEATNGATKETSMAVSLKYVVETSKCWESSVTAGWGVTTSIKAGIPEIAEAGIEINYNEEKSHTWGETITEMKEVMVTYTVSVPAKTRMKVTLLGTKAKCDVPFSYTRRDVQRDGKQVITDCDDGLYVGVNSYKFDYQNKPLPL
Homology
BLAST of Bhi12M002205 vs. ExPASy Swiss-Prot
Match: Q66S25 (Natterin-1 OS=Thalassophryne nattereri OX=289382 PE=1 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 6.1e-13
Identity = 53/178 (29.78%), Postives = 87/178 (48.88%), Query Frame = 0

Query: 267 ENKEHCL-----SAHSNTISREAQLEIVEPIISREIYNCKYRTMDARVYDEKVLNMATGE 326
           E   HCL      A + T + +A L + + +I + + + KY+T    V   K   M    
Sbjct: 144 EPAHHCLYYGWNGAETWTKTYQA-LTVNKDVIEQTMKDVKYQTEGVTVIQGKPEVMRKST 203

Query: 327 ATNGATKETSMAVSLKYVVETSKCWESSVTAGWGVTTSIKAGIPEIAEAGIEINYNEEKS 386
             N   KE +  V+L   + T + W+ + +  +GVTT++ AGIP++A A + ++    + 
Sbjct: 204 VNNKQCKEVTKTVTLSKDISTEERWDVTNSVTFGVTTTVTAGIPDVASASLAVSMEARRD 263

Query: 387 HTWGETITEMKEVMVTYTVSVPAKTRMKVTLLGTKAKCDVPFSYTRRDVQRDGKQVIT 440
              G + TE +  MVT +V VP K    V+++    K DVPF+ T     R GK+  T
Sbjct: 264 FAHGASKTESQSYMVTVSVPVPPKQSCTVSMVAQVNKADVPFTATLIRTYRGGKKTQT 320

BLAST of Bhi12M002205 vs. ExPASy Swiss-Prot
Match: Q66S21 (Natterin-2 OS=Thalassophryne nattereri OX=289382 PE=1 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 3.0e-12
Identity = 53/178 (29.78%), Postives = 87/178 (48.88%), Query Frame = 0

Query: 267 ENKEHCL-----SAHSNTISREAQLEIVEPIISREIYNCKYRTMDARVYDEKVLNMATGE 326
           E   HCL      A + T + +A L + + +I + + + KY+T    V   K   M    
Sbjct: 145 EPANHCLYYVWDGAETWTKTYQA-LTMNKDVIEQAMKDVKYQTEGVTVIKGKPEVMRRST 204

Query: 327 ATNGATKETSMAVSLKYVVETSKCWESSVTAGWGVTTSIKAGIPEIAEAGIEINYNEEKS 386
             N   KE +  V+L   + T + W+ + +  +GVTT++ AGIP+++ A +EI+      
Sbjct: 205 VNNQHCKEVTKTVTLTKDISTDERWDVTNSVTFGVTTTVTAGIPDVSSASLEISMQATMD 264

Query: 387 HTWGETITEMKEVMVTYTVSVPAKTRMKVTLLGTKAKCDVPFSYTRRDVQRDGKQVIT 440
              G + TE +  MVT +V VP K    V+++    K D+PF+ T     R GK+  T
Sbjct: 265 FAHGASKTETQSYMVTVSVPVPPKQSCTVSMVAQVNKADIPFTATLIRTYRGGKKTQT 321

BLAST of Bhi12M002205 vs. ExPASy Swiss-Prot
Match: Q66S17 (Natterin-3 OS=Thalassophryne nattereri OX=289382 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 3.7e-10
Identity = 40/154 (25.97%), Postives = 75/154 (48.70%), Query Frame = 0

Query: 286 LEIVEPIISREIYNCKYRTMDARVYDEKVLNMATGEATNGATKETSMAVSLKYVVETSKC 345
           L + + ++ +E+    Y+   A         +    A+N   +  +  V+L+  ++T + 
Sbjct: 181 LTVNDDVVKQELTQVNYKLDAAHPIKNPPETLRRSSASNSQCRPITKTVALEKAIQTEQS 240

Query: 346 WESSVTAGWGVTTSIKAGIPEIAEAGIEINYNEEKSHTWGETITEMKEVMVTYTVSVPAK 405
           W+ + T  +GV +SI AGIP+IA A + ++     S + G T T+     V+  V+VP  
Sbjct: 241 WDVTSTVTFGVESSITAGIPDIASATVSVSVETSLSVSLGSTTTKTTTHTVSVIVTVPPN 300

Query: 406 TRMKVTLLGTKAKCDVPFSYTRRDVQRDGKQVIT 440
               VT++ TK   D+PF+       R+G++  T
Sbjct: 301 HYCPVTMVATKYTADIPFTGKMTRTYRNGQKRTT 334

BLAST of Bhi12M002205 vs. ExPASy Swiss-Prot
Match: Q66S13 (Natterin-4 OS=Thalassophryne nattereri OX=289382 PE=2 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 1.6e-08
Identity = 38/161 (23.60%), Postives = 77/161 (47.83%), Query Frame = 0

Query: 286 LEIVEPIISREIYNCKYRTMDARVYDEKVLNMATGEATNGATKETSMAVSLKYVVETSKC 345
           L +   ++ ++I N +Y      V+ +K   + +    N   +E +  V+L+   ETS+ 
Sbjct: 204 LNVNMDVVEQKITNVRYNMKGVEVHKDKPETLRSTSVKNYQCREATKQVTLEKSTETSQS 263

Query: 346 WESSVTAGWGVTTSIKAGIPEIAEAGIEINYNEEKSHTWGETITEMKEVMVTYTVSVPAK 405
           W+ S +   GV+T + AGIP IA+  + ++       + G + TE     ++ + ++P  
Sbjct: 264 WDVSNSITLGVSTEVSAGIPNIADVSVAVSAETSVEISHGTSKTESTSHSLSVSATIPPN 323

Query: 406 TRMKVTLLGTKAKCDVPFSYTRRDVQRDGKQVITDCDDGLY 447
           +   +T+ G   K ++PF+        +GK V +    G+Y
Sbjct: 324 SSCSITMEGCTFKANIPFTGRLTRKYSNGK-VTSSSVKGIY 363

BLAST of Bhi12M002205 vs. ExPASy Swiss-Prot
Match: Q5CZR5 (Aerolysin-like protein OS=Danio rerio OX=7955 GN=aep1 PE=1 SV=1)

HSP 1 Score: 47.4 bits (111), Expect = 5.2e-04
Identity = 43/139 (30.94%), Postives = 68/139 (48.92%), Query Frame = 0

Query: 321 EATNGATKETSMAVSLKYVVETSKCWESSVTAGWGVTTS--------IKAGIPEIAE--A 380
           E     + E   +V  +  VETSK  +   T+ W +T S        + AGIPEIAE   
Sbjct: 168 EEIKSVSFENKTSVKQEQKVETSK--KVIKTSSWSMTKSFSSTFSVEVSAGIPEIAEVST 227

Query: 381 GIEINYNEEKSHTWGETITEMKEVMVTYTVSVPAKTRMKVTLLGTKAKCDVPFSYTRRDV 440
           G  I++  E +H+  +  T+ K   +T TV VP K ++ V +   +A  D+P++ T +  
Sbjct: 228 GFSISFGVESTHSLEQ--TDEKNETLTTTVEVPPKKKVDVHITIGRASFDLPYTGTVKIT 287

Query: 441 QRDGKQVITDCDDGLYVGV 450
            ++G  V+     G Y GV
Sbjct: 288 CKNG-SVLQYETKGQYKGV 301

BLAST of Bhi12M002205 vs. NCBI nr
Match: XP_038875125.1 (uncharacterized protein LOC120067657 [Benincasa hispida])

HSP 1 Score: 961.4 bits (2484), Expect = 2.7e-276
Identity = 463/463 (100.00%), Postives = 463/463 (100.00%), Query Frame = 0

Query: 1   MGLPKYAAFKSEKAGKYLQYLHEGPIKGNLQYSSDNVANMFSKFTLEPSKTSNYVNIRCC 60
           MGLPKYAAFKSEKAGKYLQYLHEGPIKGNLQYSSDNVANMFSKFTLEPSKTSNYVNIRCC
Sbjct: 1   MGLPKYAAFKSEKAGKYLQYLHEGPIKGNLQYSSDNVANMFSKFTLEPSKTSNYVNIRCC 60

Query: 61  YNNKFWASSNDDNHMITPSLSKPAENEFLSPCTLFRIVKAPAEGAYYLLDVLRQSYVCRC 120
           YNNKFWASSNDDNHMITPSLSKPAENEFLSPCTLFRIVKAPAEGAYYLLDVLRQSYVCRC
Sbjct: 61  YNNKFWASSNDDNHMITPSLSKPAENEFLSPCTLFRIVKAPAEGAYYLLDVLRQSYVCRC 120

Query: 121 TTSKIHENCLTTRYGNQDHYYDRSHILIDFETIVMLPKHVAFKCDIEKYLCNYWYENHEY 180
           TTSKIHENCLTTRYGNQDHYYDRSHILIDFETIVMLPKHVAFKCDIEKYLCNYWYENHEY
Sbjct: 121 TTSKIHENCLTTRYGNQDHYYDRSHILIDFETIVMLPKHVAFKCDIEKYLCNYWYENHEY 180

Query: 181 LKFDSDDIGFSKVGHQVFNVGDGSIRIKCDHSNKFWVRQPNNWILANSTDTTTNNKDTLF 240
           LKFDSDDIGFSKVGHQVFNVGDGSIRIKCDHSNKFWVRQPNNWILANSTDTTTNNKDTLF
Sbjct: 181 LKFDSDDIGFSKVGHQVFNVGDGSIRIKCDHSNKFWVRQPNNWILANSTDTTTNNKDTLF 240

Query: 241 WPVKVSKNVVALRNLGNNSFCKRFTHENKEHCLSAHSNTISREAQLEIVEPIISREIYNC 300
           WPVKVSKNVVALRNLGNNSFCKRFTHENKEHCLSAHSNTISREAQLEIVEPIISREIYNC
Sbjct: 241 WPVKVSKNVVALRNLGNNSFCKRFTHENKEHCLSAHSNTISREAQLEIVEPIISREIYNC 300

Query: 301 KYRTMDARVYDEKVLNMATGEATNGATKETSMAVSLKYVVETSKCWESSVTAGWGVTTSI 360
           KYRTMDARVYDEKVLNMATGEATNGATKETSMAVSLKYVVETSKCWESSVTAGWGVTTSI
Sbjct: 301 KYRTMDARVYDEKVLNMATGEATNGATKETSMAVSLKYVVETSKCWESSVTAGWGVTTSI 360

Query: 361 KAGIPEIAEAGIEINYNEEKSHTWGETITEMKEVMVTYTVSVPAKTRMKVTLLGTKAKCD 420
           KAGIPEIAEAGIEINYNEEKSHTWGETITEMKEVMVTYTVSVPAKTRMKVTLLGTKAKCD
Sbjct: 361 KAGIPEIAEAGIEINYNEEKSHTWGETITEMKEVMVTYTVSVPAKTRMKVTLLGTKAKCD 420

Query: 421 VPFSYTRRDVQRDGKQVITDCDDGLYVGVNSYKFDYQNKPLPL 464
           VPFSYTRRDVQRDGKQVITDCDDGLYVGVNSYKFDYQNKPLPL
Sbjct: 421 VPFSYTRRDVQRDGKQVITDCDDGLYVGVNSYKFDYQNKPLPL 463

BLAST of Bhi12M002205 vs. NCBI nr
Match: XP_004140600.1 (uncharacterized protein LOC101212789 [Cucumis sativus] >KGN46321.1 hypothetical protein Csa_004899 [Cucumis sativus])

HSP 1 Score: 741.9 bits (1914), Expect = 3.4e-210
Identity = 338/462 (73.16%), Postives = 400/462 (86.58%), Query Frame = 0

Query: 1   MGLPKYAAFKSEKAGKYLQYLHEGPIKGNLQYSSDNVANMFSKFTLEPSKTSNYVNIRCC 60
           MGLPKY AF+++ AGK+LQY+HEG +KG LQYSSDN  +MFSKFTLE SKT+NY +IRCC
Sbjct: 1   MGLPKYGAFRNKIAGKFLQYIHEGSLKGYLQYSSDNAMSMFSKFTLEQSKTTNYFHIRCC 60

Query: 61  YNNKFWASSNDDNHMITPSLSKPAENEFLSPCTLFRIVKAPAEGAYYLLDVLRQSYVCRC 120
           YNNK+WAS+ D +HM++P +SKP+ENEF  PCTLFR V+AP EG YYL DV RQSYVCRC
Sbjct: 61  YNNKYWASAKDGDHMVSPFVSKPSENEFSWPCTLFRFVQAPTEGTYYLFDVWRQSYVCRC 120

Query: 121 TTSKIHENCLTTRYGNQDHYYDRSHILIDFETIVMLPKHVAFKCDIEKYLCNYWYENHEY 180
           TTSK HE+CLTTRYGNQDHYYDR+HILIDFET+++LPKH+  K D+++YLC YWYE +E+
Sbjct: 121 TTSKSHEDCLTTRYGNQDHYYDRTHILIDFETLIVLPKHIVIKGDLKQYLCTYWYEENEH 180

Query: 181 LKFDSDDIGFSKVGHQVFNVGDGSIRIKCDHSNKFWVRQPNNWILANSTDTTTNNKDTLF 240
           LKF+SDDIG S+ GHQVF++GDGSIRIKCDH NKFWVRQ NNWILA STDTT NNKDT+F
Sbjct: 181 LKFNSDDIGSSRAGHQVFSMGDGSIRIKCDHWNKFWVRQSNNWILAKSTDTTANNKDTVF 240

Query: 241 WPVKVSKNVVALRNLGNNSFCKRFTHENKEHCLSAHSNTISREAQLEIVEPIISREIYNC 300
           WP+K+S+N VALR LGNN  CKR T ENKEHCLSA+++TI+REA+LEI EP+ISRE+YNC
Sbjct: 241 WPIKISQNTVALRCLGNNKICKRLTAENKEHCLSANADTITREARLEITEPVISREMYNC 300

Query: 301 KYRTMDARVYDEKVLNMATGEATNGATKETSMAVSLKYVVETSKCWESSVTAGWGVTTSI 360
            YRTMD+RVYDE+VL MAT  ATNG TKE SMA+SLKYVVE SKCWESSV+  WGV  S+
Sbjct: 301 NYRTMDSRVYDEQVLIMATEHATNGGTKEASMALSLKYVVEASKCWESSVSTAWGVVASV 360

Query: 361 KAGIPEIAEAGIEINYNEEKSHTWGETITEMKEVMVTYTVSVPAKTRMKVTLLGTKAKCD 420
           KAG+PEI E G+E+N+NEEKS +WGETIT+M+EVM TYTV+VP  T+MKVTL+ TKAKCD
Sbjct: 361 KAGVPEIFEVGVEVNHNEEKSRSWGETITDMREVMATYTVNVPPMTKMKVTLIATKAKCD 420

Query: 421 VPFSYTRRDVQRDGKQVITDCDDGLYVGVNSYKFDYQNKPLP 463
           VPFSYT+RDV RDG  VI DCDDGLY GV++Y FDY+NKP P
Sbjct: 421 VPFSYTQRDVLRDGTHVIKDCDDGLYTGVHTYNFDYKNKPFP 462

BLAST of Bhi12M002205 vs. NCBI nr
Match: XP_038904200.1 (uncharacterized protein LOC120090546 [Benincasa hispida])

HSP 1 Score: 607.4 bits (1565), Expect = 1.0e-169
Identity = 292/463 (63.07%), Postives = 364/463 (78.62%), Query Frame = 0

Query: 1   MGLPKYAAFKSEKAGKYLQYLHEGPIKGNLQYSSDNVANMFSKFTLEPSKTSNYVNIRCC 60
           M +P+YA FK++  GKYLQYL EG +KGNLQYSS+ V ++ +KF LEP+K S Y +IRCC
Sbjct: 1   MSIPRYAGFKNQSEGKYLQYLPEGHLKGNLQYSSNIVVSLLTKFALEPAKKSRYWHIRCC 60

Query: 61  YNNKFWASSNDDNHMITPSLSKPAENEFLSPCTLFRIVKAPAEGAYYLLDVLRQSYVCRC 120
           YNNK+ ASSND N ++TPS+SKP E+E   PCTLF IV  P  G YYL+DVL Q+YVCRC
Sbjct: 61  YNNKYLASSNDHNRLVTPSVSKPTEDESSHPCTLFNIVPGPTPGTYYLVDVLLQTYVCRC 120

Query: 121 TTSKIHENCLTTRYGNQDHYYDRSHILIDFETIVMLPKHVAFKCDIEKYLCNYWYENHEY 180
           TTSKIH+NCLTTRY   + + D+ H+  DFE  ++LPKHVAFK D   +L  +WY +  Y
Sbjct: 121 TTSKIHQNCLTTRYDVIEKHSDKLHMFTDFENFIVLPKHVAFKGDNGLFLAAFWYNDWPY 180

Query: 181 LKFDSDDIGFSKVGHQVFNVGDGSIRIKCDHSNKFWVRQPNNWILANSTDTTTNNKDTLF 240
           L+F+SDDIG   VG++VFNVGDG+IRIK D+ NKFW+R P NWILA++ DTT N+K+TLF
Sbjct: 181 LQFNSDDIGSPWVGNEVFNVGDGTIRIKNDYFNKFWIRNP-NWILADTDDTTANDKNTLF 240

Query: 241 WPVKVSKNVVALRNLGNNSFCKRFTHENKEHCLSAHSNTISREAQLEIVEPIISREIYNC 300
           WPVK+SKN VALR+LGN+ +C+R+T + K  CL++   +I +EA+ EIVEPIISREIYNC
Sbjct: 241 WPVKISKNKVALRSLGNDMYCRRYTADGKVDCLNSRVPSIIKEAEFEIVEPIISREIYNC 300

Query: 301 KYRTMDARVYDEKVLNMATGEATNGATKETSMAVSLKYVVETSKCWESSVTAGWGVTTSI 360
           KYRTMDARVYDE ++ MA  EA NG+TKE +MA+S KY  E +K WES ++    V TS+
Sbjct: 301 KYRTMDARVYDETIVTMAIEEAVNGSTKEATMALSFKYTEERTKSWESVLSMSMNVKTSV 360

Query: 361 KAGIPEIAEAGIEINYNEEKSHTWGETITEMKEVMVTYTVSVPAKTRMKVTLLGTKAKCD 420
            AGIPEIA+AGIEIN + + S+TWGETI+E KEV  TYTV+VP KTRMKVTLL TKAK D
Sbjct: 361 TAGIPEIAQAGIEINLHFDASYTWGETISETKEVTETYTVTVPEKTRMKVTLLATKAKTD 420

Query: 421 VPFSYTRRDVQRDGKQVITDCDDGLYVGVNSYKFDYQNKPLPL 464
           VPFSYTRRDV R+GK+V T+CDDGL+VG N+YK  Y+NK LPL
Sbjct: 421 VPFSYTRRDVLRNGKRVTTECDDGLFVGANTYKVVYENKALPL 462

BLAST of Bhi12M002205 vs. NCBI nr
Match: XP_038882401.1 (uncharacterized protein LOC120073669 [Benincasa hispida])

HSP 1 Score: 560.8 bits (1444), Expect = 1.1e-155
Identity = 272/464 (58.62%), Postives = 347/464 (74.78%), Query Frame = 0

Query: 3   LPKYAAFKSEKAGKYLQYLHEGPIKGNLQYSSDNVANMFSKFTLEPSKTS--NYVNIRCC 62
           LPKY AF+     KYLQYL  GP+ G+LQYSSDN+ ++++KF LEP+K+   NYVNIRCC
Sbjct: 5   LPKYVAFRDRDLKKYLQYLDHGPVSGSLQYSSDNMVSVYTKFELEPAKSGEINYVNIRCC 64

Query: 63  YNNKFWASSNDDNHMITPSLSKPAENEFLSPCTLFRIVK-APAEGAYYLLDVLRQSYVCR 122
           YNNK+ ASSNDD +++TP + KP E+    PCTLF++     +EG Y L DV RQ  VC 
Sbjct: 65  YNNKYLASSNDDKYLVTPFVDKPKEDVKSWPCTLFKVGNYISSEGYYTLFDVWRQRLVCP 124

Query: 123 CTTSKIHENCLTTRYGNQDHYYDRSHILIDFETIVMLPKHVAFKCDIEKYLCNYWYENHE 182
           C  S  ++ CLTTRYG+ +    + H  IDFE +++LPK VAFK D   YL  Y+Y + E
Sbjct: 125 CINSNNYDACLTTRYGSNEGNVQKIHQFIDFEALLVLPKRVAFKGDNGLYLAEYYYNDWE 184

Query: 183 YLKFDSDDIGFSKVGHQVFNVGDGSIRIKCDHSNKFWVRQPNNWILANSTDTTTNNKDTL 242
           Y++F+ DDIG S V HQVF + DGSIRIKC+ S  FW R P NWILA+S+DTT N+ +TL
Sbjct: 185 YMQFNCDDIGSSYVSHQVFTMADGSIRIKCEKSGMFWRRDP-NWILADSSDTTANDVNTL 244

Query: 243 FWPVKVSKNVVALRNLGNNSFCKRFTHENKEHCLSAHSNTISREAQLEIVEPIISREIYN 302
           FWPVKVSKN VALR+L NN+FCKR T ++KE CL+A + TI++EA LE+VEP+ISREIYN
Sbjct: 245 FWPVKVSKNTVALRSLANNNFCKRLTADDKEDCLNAAAETITKEATLEVVEPVISREIYN 304

Query: 303 CKYRTMDARVYDEKVLNMATGEATNGATKETSMAVSLKYVVETSKCWESSVTAGWGVTTS 362
            +YRTM+AR+YDEKV++MATG+ATNG T+ TSM + L Y  E +K W SS+T   G+TTS
Sbjct: 305 VQYRTMNARIYDEKVVSMATGDATNGGTEPTSMEIDLNYKKEKTKSWNSSLTMSLGITTS 364

Query: 363 IKAGIPEIAEAGIEINYNEEKSHTWGETITEMKEVMVTYTVSVPAKTRMKVTLLGTKAKC 422
           +KAG+P I EAGIE   +   ++ WGET++  +EV  TYTV+VPA+TRMKVTLL T   C
Sbjct: 365 VKAGVPGIGEAGIETTLSFSGTYEWGETLSTSEEVGTTYTVNVPAQTRMKVTLLATMGTC 424

Query: 423 DVPFSYTRRDVQRDGKQVITDCDDGLYVGVNSYKFDYQNKPLPL 464
           DVPFSYT+RDV R G+QVIT+CDDG+Y+G N Y F Y+NKPLPL
Sbjct: 425 DVPFSYTQRDVLRSGEQVITNCDDGVYIGANCYNFKYENKPLPL 467

BLAST of Bhi12M002205 vs. NCBI nr
Match: XP_004145086.3 (uncharacterized protein LOC105434387 [Cucumis sativus])

HSP 1 Score: 556.6 bits (1433), Expect = 2.0e-154
Identity = 271/462 (58.66%), Postives = 352/462 (76.19%), Query Frame = 0

Query: 3   LPKYAAFKSEKAGKYLQYLHEGPIKGNLQYSSDNVANMFSKFTLEPSKTSNYVNIRCCYN 62
           LPKYA F++   GKYLQYL +G ++GNLQYSSD     F+KF + P K   Y +I+CCYN
Sbjct: 4   LPKYAGFQNHVEGKYLQYLSDGFLRGNLQYSSDQALTPFTKFEIVPDKDGKYWHIKCCYN 63

Query: 63  NKFWASSNDDNHMITPSLSKPAENEFLSPCTLFRIVKAPAEGAYYLLDVLRQSYVCRCTT 122
           +K+ ASSND N  +TP +SKP+ENE   PCTLF  +  P  G YYL DVL Q+Y  RCT+
Sbjct: 64  DKYLASSNDHNRFVTPFVSKPSENESSWPCTLFNFIPGPTTGTYYLFDVLLQTYARRCTS 123

Query: 123 SKIHENCLTTRY-GNQDHYYDRSHILIDFETIVMLPKHVAFKCDIEKYLCNYWYENHEYL 182
           S+IH + LTTRY  ++  + D+  +L+DFE I+ LPK+++FK + +K+L  Y Y N  YL
Sbjct: 124 SRIHPDVLTTRYRRDEKRHDDKLVVLVDFENIIRLPKYLSFK-NNDKFLATYTYRNSPYL 183

Query: 183 KFDSDDIGFSKVGHQVFNVGDGSIRIKCDHSNKFWVRQPNNWILANSTDTTTNNKDTLFW 242
           +F+++DIG  +V H++FNVGDG++RIK + + KFW R P NWILA+S D T N+K+TLFW
Sbjct: 184 QFNANDIGNPQVSHEIFNVGDGTVRIKNESTKKFWRRDP-NWILADSNDQTKNDKNTLFW 243

Query: 243 PVKVSKNVVALRNLGNNSFCKRFTHENKEHCLSAHSNTISREAQLEIVEPIISREIYNCK 302
           PVK++KN VALRN+ N  F  R+T + K   L+A S TI++EA+LEIVEPIISREIYN +
Sbjct: 244 PVKIAKNKVALRNVANGRFVTRYTGDGKVDFLNASSETITKEAELEIVEPIISREIYNIR 303

Query: 303 YRTMDARVYDEKVLNMATGEATNGATKETSMAVSLKYVVETSKCWESSVTAGWGVTTSIK 362
           YRTMDA++YDE+VL MAT EA N ++KET MAVSL+Y+ E SK WESS+T G G+ TSIK
Sbjct: 304 YRTMDAKIYDEQVLTMATEEAVNSSSKETVMAVSLRYLEEKSKTWESSLTVGVGIETSIK 363

Query: 363 AGIPEIAEAGIEINYNEEKSHTWGETITEMKEVMVTYTVSVPAKTRMKVTLLGTKAKCDV 422
           AGIPEI +  I+++YN E S+TWGETI+E +EV  TYTV VP  TRMKVTLL TKAKCD+
Sbjct: 364 AGIPEILDTEIKVSYNFEASYTWGETISESREVTATYTVPVPPNTRMKVTLLATKAKCDI 423

Query: 423 PFSYTRRDVQRDGKQVITDCDDGLYVGVNSYKFDYQNKPLPL 464
           PFSYT+RD+ R+G++V T+ DDGLY GV ++KFDYQNKPLPL
Sbjct: 424 PFSYTQRDLLRNGRRVETEYDDGLYTGVYTFKFDYQNKPLPL 463

BLAST of Bhi12M002205 vs. ExPASy TrEMBL
Match: A0A0A0KEW7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G083560 PE=4 SV=1)

HSP 1 Score: 741.9 bits (1914), Expect = 1.6e-210
Identity = 338/462 (73.16%), Postives = 400/462 (86.58%), Query Frame = 0

Query: 1   MGLPKYAAFKSEKAGKYLQYLHEGPIKGNLQYSSDNVANMFSKFTLEPSKTSNYVNIRCC 60
           MGLPKY AF+++ AGK+LQY+HEG +KG LQYSSDN  +MFSKFTLE SKT+NY +IRCC
Sbjct: 1   MGLPKYGAFRNKIAGKFLQYIHEGSLKGYLQYSSDNAMSMFSKFTLEQSKTTNYFHIRCC 60

Query: 61  YNNKFWASSNDDNHMITPSLSKPAENEFLSPCTLFRIVKAPAEGAYYLLDVLRQSYVCRC 120
           YNNK+WAS+ D +HM++P +SKP+ENEF  PCTLFR V+AP EG YYL DV RQSYVCRC
Sbjct: 61  YNNKYWASAKDGDHMVSPFVSKPSENEFSWPCTLFRFVQAPTEGTYYLFDVWRQSYVCRC 120

Query: 121 TTSKIHENCLTTRYGNQDHYYDRSHILIDFETIVMLPKHVAFKCDIEKYLCNYWYENHEY 180
           TTSK HE+CLTTRYGNQDHYYDR+HILIDFET+++LPKH+  K D+++YLC YWYE +E+
Sbjct: 121 TTSKSHEDCLTTRYGNQDHYYDRTHILIDFETLIVLPKHIVIKGDLKQYLCTYWYEENEH 180

Query: 181 LKFDSDDIGFSKVGHQVFNVGDGSIRIKCDHSNKFWVRQPNNWILANSTDTTTNNKDTLF 240
           LKF+SDDIG S+ GHQVF++GDGSIRIKCDH NKFWVRQ NNWILA STDTT NNKDT+F
Sbjct: 181 LKFNSDDIGSSRAGHQVFSMGDGSIRIKCDHWNKFWVRQSNNWILAKSTDTTANNKDTVF 240

Query: 241 WPVKVSKNVVALRNLGNNSFCKRFTHENKEHCLSAHSNTISREAQLEIVEPIISREIYNC 300
           WP+K+S+N VALR LGNN  CKR T ENKEHCLSA+++TI+REA+LEI EP+ISRE+YNC
Sbjct: 241 WPIKISQNTVALRCLGNNKICKRLTAENKEHCLSANADTITREARLEITEPVISREMYNC 300

Query: 301 KYRTMDARVYDEKVLNMATGEATNGATKETSMAVSLKYVVETSKCWESSVTAGWGVTTSI 360
            YRTMD+RVYDE+VL MAT  ATNG TKE SMA+SLKYVVE SKCWESSV+  WGV  S+
Sbjct: 301 NYRTMDSRVYDEQVLIMATEHATNGGTKEASMALSLKYVVEASKCWESSVSTAWGVVASV 360

Query: 361 KAGIPEIAEAGIEINYNEEKSHTWGETITEMKEVMVTYTVSVPAKTRMKVTLLGTKAKCD 420
           KAG+PEI E G+E+N+NEEKS +WGETIT+M+EVM TYTV+VP  T+MKVTL+ TKAKCD
Sbjct: 361 KAGVPEIFEVGVEVNHNEEKSRSWGETITDMREVMATYTVNVPPMTKMKVTLIATKAKCD 420

Query: 421 VPFSYTRRDVQRDGKQVITDCDDGLYVGVNSYKFDYQNKPLP 463
           VPFSYT+RDV RDG  VI DCDDGLY GV++Y FDY+NKP P
Sbjct: 421 VPFSYTQRDVLRDGTHVIKDCDDGLYTGVHTYNFDYKNKPFP 462

BLAST of Bhi12M002205 vs. ExPASy TrEMBL
Match: A0A0A0KEW1 (Agglutinin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G082530 PE=4 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 2.2e-154
Identity = 270/462 (58.44%), Postives = 352/462 (76.19%), Query Frame = 0

Query: 3   LPKYAAFKSEKAGKYLQYLHEGPIKGNLQYSSDNVANMFSKFTLEPSKTSNYVNIRCCYN 62
           LPKYA F++   GKYLQYL +G ++GNLQYSSD     F+KF + P K   Y +I+CCYN
Sbjct: 4   LPKYAGFQNHVEGKYLQYLSDGFLRGNLQYSSDQALTPFTKFEIVPDKDGKYWHIKCCYN 63

Query: 63  NKFWASSNDDNHMITPSLSKPAENEFLSPCTLFRIVKAPAEGAYYLLDVLRQSYVCRCTT 122
           +K+ ASSND N  +TP +SKP+ENE   PCTLF  +  P  G YYL DVL Q+Y  RCT+
Sbjct: 64  DKYLASSNDHNRFVTPFVSKPSENESSWPCTLFNFIPGPTTGTYYLFDVLLQTYARRCTS 123

Query: 123 SKIHENCLTTRY-GNQDHYYDRSHILIDFETIVMLPKHVAFKCDIEKYLCNYWYENHEYL 182
           S+IH + LTTRY  ++  + D+  +L+DFE I+ LPK+++FK + +K+L  Y Y N  YL
Sbjct: 124 SRIHPDVLTTRYRRDEKRHDDKLVVLVDFENIIRLPKYLSFK-NNDKFLATYTYRNSPYL 183

Query: 183 KFDSDDIGFSKVGHQVFNVGDGSIRIKCDHSNKFWVRQPNNWILANSTDTTTNNKDTLFW 242
           +F+++DIG  +V H++FNVGDG+++IK + + KFW R P NWILA+S D T N+K+TLFW
Sbjct: 184 QFNANDIGNPQVSHEIFNVGDGTVKIKNESTKKFWRRDP-NWILADSNDQTKNDKNTLFW 243

Query: 243 PVKVSKNVVALRNLGNNSFCKRFTHENKEHCLSAHSNTISREAQLEIVEPIISREIYNCK 302
           PVK++KN VALRN+ N  F  R+T + K   L+A S TI++EA+LEIVEPIISREIYN +
Sbjct: 244 PVKIAKNKVALRNVANGRFVTRYTGDGKVDFLNASSETITKEAELEIVEPIISREIYNIR 303

Query: 303 YRTMDARVYDEKVLNMATGEATNGATKETSMAVSLKYVVETSKCWESSVTAGWGVTTSIK 362
           YRTMDA++YDE+VL MAT EA N ++KET MAVSL+Y+ E SK WESS+T G G+ TSIK
Sbjct: 304 YRTMDAKIYDEQVLTMATEEAVNSSSKETVMAVSLRYLEEKSKTWESSLTVGVGIETSIK 363

Query: 363 AGIPEIAEAGIEINYNEEKSHTWGETITEMKEVMVTYTVSVPAKTRMKVTLLGTKAKCDV 422
           AGIPEI +  I+++YN E S+TWGETI+E +EV  TYTV VP  TRMKVTLL TKAKCD+
Sbjct: 364 AGIPEILDTEIKVSYNFEASYTWGETISESREVTATYTVPVPPNTRMKVTLLATKAKCDI 423

Query: 423 PFSYTRRDVQRDGKQVITDCDDGLYVGVNSYKFDYQNKPLPL 464
           PFSYT+RD+ R+G++V T+ DDGLY GV ++KFDYQNKPLPL
Sbjct: 424 PFSYTQRDLLRNGRRVETEYDDGLYTGVYTFKFDYQNKPLPL 463

BLAST of Bhi12M002205 vs. ExPASy TrEMBL
Match: A0A1S4E3C5 (uncharacterized protein LOC103498965 OS=Cucumis melo OX=3656 GN=LOC103498965 PE=4 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 1.6e-149
Identity = 261/462 (56.49%), Postives = 350/462 (75.76%), Query Frame = 0

Query: 3   LPKYAAFKSEKAGKYLQYLHEGPIKGNLQYSSDNVANMFSKFTLEPSKTSNYVNIRCCYN 62
           LPKYA F++   GKYLQY+ +G ++GNLQYSSD     F+KF + P K   Y +I+CCYN
Sbjct: 4   LPKYAGFQNHAEGKYLQYIPDGHLRGNLQYSSDQALTPFTKFEIVPDKDGKYWHIKCCYN 63

Query: 63  NKFWASSNDDNHMITPSLSKPAENEFLSPCTLFRIVKAPAEGAYYLLDVLRQSYVCRCTT 122
           +K+ ASS+D N  +TP +SKP+ENE   PCT+F I+  P  G YYL DV  Q+Y  RCT+
Sbjct: 64  DKYLASSDDHNRFVTPFVSKPSENESSRPCTMFNIISGPTTGTYYLFDVSLQTYARRCTS 123

Query: 123 SKIHENCLTTRY-GNQDHYYDRSHILIDFETIVMLPKHVAFKCDIEKYLCNYWYENHEYL 182
           S+IH N LTTRY  +++ + D+  +L+DFE I+ LPK+++FK + +K+L  + Y +  YL
Sbjct: 124 SRIHPNVLTTRYRRDENRHDDKLVLLVDFENIIRLPKYLSFK-NNDKFLAAHTYYDSPYL 183

Query: 183 KFDSDDIGFSKVGHQVFNVGDGSIRIKCDHSNKFWVRQPNNWILANSTDTTTNNKDTLFW 242
           KF +DDIG   V H++FNVGDG++RIK + +NKFW R P NWILA+S D T ++K+TLFW
Sbjct: 184 KFKADDIGNPLVSHEIFNVGDGTVRIKNESTNKFWRRDP-NWILADSNDQTAHDKNTLFW 243

Query: 243 PVKVSKNVVALRNLGNNSFCKRFTHENKEHCLSAHSNTISREAQLEIVEPIISREIYNCK 302
           PVK++K+ VALRN+ N  F  R+T + K   L+A S TI+++A+LEI EP+ISRE+ N K
Sbjct: 244 PVKIAKDKVALRNVANGRFVTRYTGDGKVDFLNASSETITKQAELEIYEPVISRELCNFK 303

Query: 303 YRTMDARVYDEKVLNMATGEATNGATKETSMAVSLKYVVETSKCWESSVTAGWGVTTSIK 362
           YRTMDA++YDE+VL MAT EA N ++KET+MA+SL+Y+ E+SK WESS+T G G+  SIK
Sbjct: 304 YRTMDAKIYDEQVLTMATEEAVNTSSKETTMAISLRYLEESSKTWESSLTVGVGIEASIK 363

Query: 363 AGIPEIAEAGIEINYNEEKSHTWGETITEMKEVMVTYTVSVPAKTRMKVTLLGTKAKCDV 422
           AGIP + E+ I+++YN E S+ WGETI+E KEV  TYTV VP KTRMKVTLL TKAKCD+
Sbjct: 364 AGIPLLLESEIKVSYNFEGSYKWGETISETKEVTSTYTVIVPPKTRMKVTLLATKAKCDI 423

Query: 423 PFSYTRRDVQRDGKQVITDCDDGLYVGVNSYKFDYQNKPLPL 464
           PFSYT+RD+ R+G++V  +CDDGLY GV ++KFDYQNK LPL
Sbjct: 424 PFSYTQRDLLRNGRRVEIECDDGLYTGVYTFKFDYQNKSLPL 463

BLAST of Bhi12M002205 vs. ExPASy TrEMBL
Match: A0A5D3DLV4 (Agglutinin domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001160 PE=3 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 3.6e-149
Identity = 260/462 (56.28%), Postives = 351/462 (75.97%), Query Frame = 0

Query: 3   LPKYAAFKSEKAGKYLQYLHEGPIKGNLQYSSDNVANMFSKFTLEPSKTSNYVNIRCCYN 62
           LPKYA F++   GKYLQY+ +G ++GNLQYSSD     F+KF + P K   Y +I+CCYN
Sbjct: 4   LPKYAGFQNHAEGKYLQYIPDGHLRGNLQYSSDQALTPFTKFEIVPDKDGKYWHIKCCYN 63

Query: 63  NKFWASSNDDNHMITPSLSKPAENEFLSPCTLFRIVKAPAEGAYYLLDVLRQSYVCRCTT 122
           +K+ ASS+D N  +TP +SKP+ENE   PCT+F I+  P  G YYL DV  Q+Y  RCT+
Sbjct: 64  DKYLASSDDHNRFVTPFVSKPSENESSRPCTMFNIISGPTTGTYYLFDVSLQTYARRCTS 123

Query: 123 SKIHENCLTTRY-GNQDHYYDRSHILIDFETIVMLPKHVAFKCDIEKYLCNYWYENHEYL 182
           S+IH + LTTRY  +++ + D+  +L+DFE I+ LPK+++FK + +K+L  + Y +  YL
Sbjct: 124 SRIHPDVLTTRYRRDENRHDDKLVLLVDFENIIRLPKYLSFK-NNDKFLAAHTYYDSPYL 183

Query: 183 KFDSDDIGFSKVGHQVFNVGDGSIRIKCDHSNKFWVRQPNNWILANSTDTTTNNKDTLFW 242
           KF +DDIG   V H++FNVGDG++RIK + +NKFW R P NWILA+S D T ++K+TLFW
Sbjct: 184 KFKADDIGNPLVSHEIFNVGDGTVRIKNESTNKFWRRDP-NWILADSNDQTAHDKNTLFW 243

Query: 243 PVKVSKNVVALRNLGNNSFCKRFTHENKEHCLSAHSNTISREAQLEIVEPIISREIYNCK 302
           PVK++K+ VALRN+ N  F  R+T + K   L+A S TI+++A+LEI EP+ISRE+ N K
Sbjct: 244 PVKIAKDKVALRNVANGRFVTRYTGDGKVDFLNASSETITKQAELEIYEPVISRELCNFK 303

Query: 303 YRTMDARVYDEKVLNMATGEATNGATKETSMAVSLKYVVETSKCWESSVTAGWGVTTSIK 362
           YRTMDA++YDE+VL MAT EA N ++KET+MA+SL+Y+ E+SK WESS+T G G+  SIK
Sbjct: 304 YRTMDAKIYDEQVLTMATEEAVNTSSKETTMAISLRYLEESSKTWESSLTVGVGIEASIK 363

Query: 363 AGIPEIAEAGIEINYNEEKSHTWGETITEMKEVMVTYTVSVPAKTRMKVTLLGTKAKCDV 422
           AGIP + E+ I+++YN E S+ WGETI+E KEV  TYTV VP+KTRMKVTLL TKAKCD+
Sbjct: 364 AGIPLLLESEIKVSYNFEGSYKWGETISETKEVTSTYTVIVPSKTRMKVTLLATKAKCDI 423

Query: 423 PFSYTRRDVQRDGKQVITDCDDGLYVGVNSYKFDYQNKPLPL 464
           PFSYT+RD+ R+G++V  +CDDGLY GV ++KFDYQNK LPL
Sbjct: 424 PFSYTQRDLLRNGRRVEIECDDGLYTGVYTFKFDYQNKSLPL 463

BLAST of Bhi12M002205 vs. ExPASy TrEMBL
Match: A0A0A0KA07 (Agglutinin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G083530 PE=4 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 2.0e-144
Identity = 252/462 (54.55%), Postives = 341/462 (73.81%), Query Frame = 0

Query: 3   LPKYAAFKSEKAGKYLQYLHEGPIKGNLQYSSDNVANMFSKFTLEPSKTSNYVNIRCCYN 62
           LPKYA F++    KYL+Y+ +G +KG L+YSSD     F+KF + P K   Y +I+CCYN
Sbjct: 4   LPKYAGFQNHAERKYLEYVRDGFLKGKLKYSSDQALTPFTKFEIVPDKDGKYWHIKCCYN 63

Query: 63  NKFWASSNDDNHMITPSLSKPAENEFLSPCTLFRIVKAPAEGAYYLLDVLRQSYVCRCTT 122
            K+ AS+ND N  + P +SKP+ENE   PCTLF I+  P  G YYL DV+  +Y CRCT 
Sbjct: 64  GKYLASTNDGNRFVAPFVSKPSENESSWPCTLFNIIPGPTIGTYYLFDVMIYTYACRCTD 123

Query: 123 SKIHENCLTTRYGNQD-HYYDRSHILIDFETIVMLPKHVAFKCDIEKYLCNYWYENHEYL 182
            ++H + LTT Y  ++    D+  +L+DFE ++ LPK+VAFK + +K L ++ Y N  YL
Sbjct: 124 RRVHHDVLTTIYNLEEARDSDKLILLVDFENVIRLPKYVAFK-NNDKLLASFSYNNSMYL 183

Query: 183 KFDSDDIGFSKVGHQVFNVGDGSIRIKCDHSNKFWVRQPNNWILANSTDTTTNNKDTLFW 242
           +F++DDIG     H+VFNVGDG+IRIK D++ KFW R P NWILA+S D T NNK+TLFW
Sbjct: 184 QFNTDDIGNPWAAHEVFNVGDGTIRIKNDYTKKFWRRDP-NWILADSNDQTKNNKNTLFW 243

Query: 243 PVKVSKNVVALRNLGNNSFCKRFTHENKEHCLSAHSNTISREAQLEIVEPIISREIYNCK 302
           PVK++KN +ALRN+ N   C+R++ ++K  CL+A +++I ++A+LEIVE +ISREIYN +
Sbjct: 244 PVKIAKNKIALRNVANGMICRRYSADSKVDCLNARADSIIKDAELEIVELVISREIYNIR 303

Query: 303 YRTMDARVYDEKVLNMATGEATNGATKETSMAVSLKYVVETSKCWESSVTAGWGVTTSIK 362
           YRTMDA++YDE+VL MAT EA N ++KET MAVSLKY+ E ++ WESS+T G  +  SIK
Sbjct: 304 YRTMDAKIYDEQVLTMATEEAINSSSKETVMAVSLKYLEEKTRSWESSLTIGAVIEASIK 363

Query: 363 AGIPEIAEAGIEINYNEEKSHTWGETITEMKEVMVTYTVSVPAKTRMKVTLLGTKAKCDV 422
           AGIPEI E+ I++ Y+ E ++TWGET+ E +EV  TYTV+VP   +MKV+LL TKAKCD+
Sbjct: 364 AGIPEILESEIKVTYSFEGNYTWGETLQETREVTSTYTVTVPPNKKMKVSLLATKAKCDI 423

Query: 423 PFSYTRRDVQRDGKQVITDCDDGLYVGVNSYKFDYQNKPLPL 464
           PFSYT+RD+ ++GK+V  DCDDGLY GV + KFDYQNK LPL
Sbjct: 424 PFSYTQRDLLQNGKKVEIDCDDGLYTGVYTIKFDYQNKTLPL 463

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
Q66S256.1e-1329.78Natterin-1 OS=Thalassophryne nattereri OX=289382 PE=1 SV=1[more]
Q66S213.0e-1229.78Natterin-2 OS=Thalassophryne nattereri OX=289382 PE=1 SV=1[more]
Q66S173.7e-1025.97Natterin-3 OS=Thalassophryne nattereri OX=289382 PE=1 SV=1[more]
Q66S131.6e-0823.60Natterin-4 OS=Thalassophryne nattereri OX=289382 PE=2 SV=1[more]
Q5CZR55.2e-0430.94Aerolysin-like protein OS=Danio rerio OX=7955 GN=aep1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_038875125.12.7e-276100.00uncharacterized protein LOC120067657 [Benincasa hispida][more]
XP_004140600.13.4e-21073.16uncharacterized protein LOC101212789 [Cucumis sativus] >KGN46321.1 hypothetical ... [more]
XP_038904200.11.0e-16963.07uncharacterized protein LOC120090546 [Benincasa hispida][more]
XP_038882401.11.1e-15558.62uncharacterized protein LOC120073669 [Benincasa hispida][more]
XP_004145086.32.0e-15458.66uncharacterized protein LOC105434387 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A0A0KEW71.6e-21073.16Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G083560 PE=4 SV=1[more]
A0A0A0KEW12.2e-15458.44Agglutinin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G082530 ... [more]
A0A1S4E3C51.6e-14956.49uncharacterized protein LOC103498965 OS=Cucumis melo OX=3656 GN=LOC103498965 PE=... [more]
A0A5D3DLV43.6e-14956.28Agglutinin domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A0A0KA072.0e-14454.55Agglutinin domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G083530 ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008998Agglutinin domainSMARTSM00791agglutinincoord: 154..290
e-value: 9.7E-29
score: 111.5
coord: 1..149
e-value: 1.6E-5
score: 24.2
IPR008998Agglutinin domainPFAMPF07468Agglutinincoord: 2..132
e-value: 4.7E-15
score: 56.1
coord: 159..288
e-value: 1.7E-13
score: 51.1
NoneNo IPR availableGENE3D2.80.10.50coord: 1..155
e-value: 1.3E-33
score: 117.8
NoneNo IPR availableGENE3D2.80.10.50coord: 156..291
e-value: 1.0E-43
score: 150.6
NoneNo IPR availableGENE3D2.170.15.10Proaerolysin, chain A, domain 3coord: 295..459
e-value: 1.1E-40
score: 141.0
NoneNo IPR availablePANTHERPTHR39244:SF5NATTERIN-4coord: 1..463
NoneNo IPR availablePANTHERPTHR39244NATTERIN-4coord: 1..463
NoneNo IPR availableCDDcd20216PFM_HFR-2-likecoord: 309..460
e-value: 3.02409E-64
score: 202.433
NoneNo IPR availableSUPERFAMILY56973Aerolisin/ETX pore-forming domaincoord: 216..457
IPR036242Agglutinin domain superfamilySUPERFAMILY50382Agglutinincoord: 152..290
IPR036242Agglutinin domain superfamilySUPERFAMILY50382Agglutinincoord: 1..146

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Bhi12G002205Bhi12G002205gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi12M002205.exon1Bhi12M002205.exon1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi12M002205.utr3p1Bhi12M002205.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Bhi12M002205cds.Bhi12M002205CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi12M002205.utr5p1Bhi12M002205.utr5p1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Bhi12M002205Bhi12M002205-proteinpolypeptide