Bhi12M002069 (mRNA) Wax gourd (B227) v1

Overview
NameBhi12M002069
TypemRNA
OrganismBenincasa hispida (Wax gourd (B227) v1)
Description1-phosphatidylinositol 4-kinase
Locationchr12: 73832696 .. 73836079 (-)
Sequence length2731
RNA-Seq ExpressionBhi12M002069
SyntenyBhi12M002069
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCTCCGATGTGTACACGCGTCTTCTTGTTCGTTGAGCGCTCGGTCTTGTTGTTCATCTCAGTCTCATCAAATCCCTAATTTTTCTGCATCAGTTTCTTCCTTCTTTGCCAATTACCAGAATTTTACACCAATTTTCTATCTTTAGAACCTAAAATTTAAAGTTCCCCACTTCTCCATTTTCCCTTTGGTTCTTCCTGTTCAGTGAGAATCCAAACCCCCCTTTTTTGCCCTTCTTAAATTCCTCTAGGTTTCTTCTTCCTCTCCTCCTCCGATCAATCCCCACTCATCTCTTCTTCTTCTCAACGACACTGACAGATCCCCGAAGCCTCTCCCAGGGTTCGTGTGGAGTTTTCACTTCCCTTGGTTTCTTTGGTAATTTTTATAATTTTTTGATCCACTTTAGGCGGAAATGGGTCGCTAGCCTTTTCATTTTTCTTTGTCCCCTTATTATTATTATTACTACTACTATTGGTTTGATCATCTTGCTTCTGTGCTCTGTGATGGTCGTTGAATCTAGAACTATGGCTATGTTGGTGTAATTTCTTGACCCTTCTGTTCTTTTGAGTATTGAAGCCCTAATTTTGGCTGTTTGATTTAATTCCTGATTCAAAATGTCGTCTACGGGTGTTACTGCTCTTTGTCCAGTTCATAATGAGCATTTATTTTCCCATAATCGTTTAATTACTCCATCCATTCTGGAATCCAATGAGTCGATTTGGATTTATGTTTCTGTTTCGGGCTCTATGAGTCCTATGCCCATCTTTGCATCGGAATCCATTGAATCTGTGAAGTTAAGGATCCAATCTTGCAAGGGATTTGTGGTGAAGAAACAGAAGTTAGTTTGTGGTGGCCGAGAATTGTCTCGAAATAATTCATTAGTTCGGGATTATGGTGTTACGGATGGAAATGTGTTGCATTTGGTGCTTAGACTATCAGATCTTCAGGTTATTAATGTCAAGACTCATTGTGGGAAAGAGTTTACCTTCCATGTGGAGCGGGACAGAGATATTGCATATGTAAAAGAGAAGATTGCTACAAAGGTAAAGGAGTTTGTGGACGTTGATGACCATGAAGTAGTGTGTGATGGAAAGCCGCTAGATGATCATAGCCTTGTAGACGATATTTGTAATAGGCAGGATGCAGTCATACATTTGTTTGTTCGGAAGTCTGCAAAAGTACGAGGCAGACCTATCGAAAAAAACTTTGAGCTCTCTATTGTGGCATCAAATTTAAAAGAACAGTGTAAATCTGAGTTTTCTAGAACAAATAATCAGAAGGAATACAATGAGGATAAGGAAGCTTATAGGACAAAGTATGAATATGACAAAGAAATTGGACCAAGAAGGCCTTCTCCTAATGGAGGTAGTGTTATGGAGCCCATTGTCATTAACCCTAAGATTGAATTGCCAATACCCATTTGGGATATGGTCAATGCTACTGTAGATGGCTTAGACCGTGGGCATTTTCCAGTTAGGTCTTTGGAGGGAACCGGTGGAGCATATCTTATGTTAGACCCATCAGGAAAGAAGTATGTGTCTGTTTTTAAGCCAATTGATGAGGAGCCAATGGCCTTGAACAATCCACGGGGACTTCCATTGTCACTAGACGGTGAAGGGTTAAAGAAAGGAACCAAAGTTGGAGAAGGAGCATTCAGAGAAGTTGCAGCTTACCTATTAGATCATCCAATAAGTGGACGCCGTTCAATGTTCGGTGACAAGAATGGCTTTGCCGGTGTTCCTCCTACAGCATTGGTCAAGTGCCTTCATGACGGTTTCAACCATCCTAGTGATAAATCAGTTAAAATTGGTTCCTTGCAGATGTTCATGGAAAATAATGGTAGTTGTGAGGACTTTGGTGCTGGGGCTTTCCCTACCAAGGAGGTGCACAAAATCTCTGTTTTGGATATTAGATTGGCAAATGCAGATAGACATGCTGGAAATATTCTATTGGGAAAAGAAAGAGAAGGGGGTCAAACTGTGCTTATTCCAATCGACCATGGATACTGCTTGCCAACAAGTGTAAGTTTTCTTTACTCTTAATTGGTTCATTGCATGTTTTAAATTTAGTCGAATTATAAGAAAACATCCTTTAATGTTGTCATTACACTTCAAATAGTAAAAATATTGCCCTGGAACTTAACAAATGTTTCAAAAATATTCTGAGTAGAAATTCTTCAAAATTATGGACGAAAATTGAGACATGAAATGATTTTGTAATGGCCTGAAATGAAAATTTAGACCAACACAACGTTTTTAGGTCATCGCCACACAGTTTTAAGTCTCGATTTCTCTTCGAAATTTTGAAGGAATTACATTCTAAGGGTAATTTTGAAACAGTTTTAAGTCTCGATTTCTCTTCAAAATTTTGAAGGAATTACATTCTAAGGGTAATTTTGAAATATTTATGAAAGTTTAAGGGTAATAAATGCAATTTTTTTAAAGTGTAGTGGTATTTTTTGAAACAAAGGGCAAAGTTCAAGGGCAGTTTAGCCTTTAAATTTTCACGAGCTGAGGAATCGAACTGTGATATTAGTCTCTAATTCTCATACTGAATTTTGTTGGCTGGAGCTTAATTTACTCGGGGAGATTTCAATATAGATTTTTGAATTAGCAGTACCAGAATCTCGTATTCAATTGAAACTTATCTGAGTCGATATTCTCTATTGTTTCAGTTTGAAGATTGTACATTTGACTGGCTCTATTGGCCACAAGCCCAGCAACCATATGATGCTGAGACATTAGACTACATAAATTCACTGGACGCAGAGGAAGATATCGCCCTTCTCAAGTTCCATGGATGGGACCTGCCTCTTGAATGTGCTCGTACACTTCGCATTTCAACCATGCTGTTGAAGAAAGGGGCAAAGAGAGGGCTCACGCCATTCGACATCGGTAGCTTTATGTGTAGAGAAACCTTGATGAAGAAATCAATGATCGAGGAGTGCGTGGAAGAAGCAATGGATTCTTTGCTTCCTGGTACGAGTGAAGCAACATTCCTTGAAAGTGTATATGAAATAATGGATCACCGCCTTGATCAGATTGCTGCTTCATTGTCCTGATGGGGAAAGCTAAGATCATTTTAGTAACTAAATAATAGGTGTAATTAATTTATTAAAATATGTGGATAATGCATATAATATGATGATGTTAATGAATTATTATTATTATCATGTACGAACGACAGGACTATATCTGTTCTGCTTCTTCGTTCTTTGTTATAGCCGTTGAGAAGGATCGTCGGCGGCTTTGTCGATGGCATTTCTTTGGAATTGCTGTTCTTATTTATTATGTTTGTATTTGCCCTCCTAAGGTCTTTTTCCTTTGGATTTTCAATGGAAATTTAATAAATATTTTATGATTTTTTTTTAATATATATTTCCCTGA

mRNA sequence

ATCTCCGATGTGTACACGCGTCTTCTTGTTCGTTGAGCGCTCGGTCTTGTTGTTCATCTCAGTCTCATCAAATCCCTAATTTTTCTGCATCAGTTTCTTCCTTCTTTGCCAATTACCAGAATTTTACACCAATTTTCTATCTTTAGAACCTAAAATTTAAAGTTCCCCACTTCTCCATTTTCCCTTTGGTTCTTCCTGTTCAGTGAGAATCCAAACCCCCCTTTTTTGCCCTTCTTAAATTCCTCTAGGTTTCTTCTTCCTCTCCTCCTCCGATCAATCCCCACTCATCTCTTCTTCTTCTCAACGACACTGACAGATCCCCGAAGCCTCTCCCAGGGTTCGTGTGGAGTTTTCACTTCCCTTGGTTTCTTTGGTAATTTTTATAATTTTTTGATCCACTTTAGGCGGAAATGGGTCGCTAGCCTTTTCATTTTTCTTTGTCCCCTTATTATTATTATTACTACTACTATTGGTTTGATCATCTTGCTTCTGTGCTCTGTGATGGTCGTTGAATCTAGAACTATGGCTATGTTGGTGTAATTTCTTGACCCTTCTGTTCTTTTGAGTATTGAAGCCCTAATTTTGGCTGTTTGATTTAATTCCTGATTCAAAATGTCGTCTACGGGTGTTACTGCTCTTTGTCCAGTTCATAATGAGCATTTATTTTCCCATAATCGTTTAATTACTCCATCCATTCTGGAATCCAATGAGTCGATTTGGATTTATGTTTCTGTTTCGGGCTCTATGAGTCCTATGCCCATCTTTGCATCGGAATCCATTGAATCTGTGAAGTTAAGGATCCAATCTTGCAAGGGATTTGTGGTGAAGAAACAGAAGTTAGTTTGTGGTGGCCGAGAATTGTCTCGAAATAATTCATTAGTTCGGGATTATGGTGTTACGGATGGAAATGTGTTGCATTTGGTGCTTAGACTATCAGATCTTCAGGTTATTAATGTCAAGACTCATTGTGGGAAAGAGTTTACCTTCCATGTGGAGCGGGACAGAGATATTGCATATGTAAAAGAGAAGATTGCTACAAAGGTAAAGGAGTTTGTGGACGTTGATGACCATGAAGTAGTGTGTGATGGAAAGCCGCTAGATGATCATAGCCTTGTAGACGATATTTGTAATAGGCAGGATGCAGTCATACATTTGTTTGTTCGGAAGTCTGCAAAAGTACGAGGCAGACCTATCGAAAAAAACTTTGAGCTCTCTATTGTGGCATCAAATTTAAAAGAACAGTGTAAATCTGAGTTTTCTAGAACAAATAATCAGAAGGAATACAATGAGGATAAGGAAGCTTATAGGACAAAGTATGAATATGACAAAGAAATTGGACCAAGAAGGCCTTCTCCTAATGGAGGTAGTGTTATGGAGCCCATTGTCATTAACCCTAAGATTGAATTGCCAATACCCATTTGGGATATGGTCAATGCTACTGTAGATGGCTTAGACCGTGGGCATTTTCCAGTTAGGTCTTTGGAGGGAACCGGTGGAGCATATCTTATGTTAGACCCATCAGGAAAGAAGTATGTGTCTGTTTTTAAGCCAATTGATGAGGAGCCAATGGCCTTGAACAATCCACGGGGACTTCCATTGTCACTAGACGGTGAAGGGTTAAAGAAAGGAACCAAAGTTGGAGAAGGAGCATTCAGAGAAGTTGCAGCTTACCTATTAGATCATCCAATAAGTGGACGCCGTTCAATGTTCGGTGACAAGAATGGCTTTGCCGGTGTTCCTCCTACAGCATTGGTCAAGTGCCTTCATGACGGTTTCAACCATCCTAGTGATAAATCAGTTAAAATTGGTTCCTTGCAGATGTTCATGGAAAATAATGGTAGTTGTGAGGACTTTGGTGCTGGGGCTTTCCCTACCAAGGAGGTGCACAAAATCTCTGTTTTGGATATTAGATTGGCAAATGCAGATAGACATGCTGGAAATATTCTATTGGGAAAAGAAAGAGAAGGGGGTCAAACTGTGCTTATTCCAATCGACCATGGATACTGCTTGCCAACAAGTTTTGAAGATTGTACATTTGACTGGCTCTATTGGCCACAAGCCCAGCAACCATATGATGCTGAGACATTAGACTACATAAATTCACTGGACGCAGAGGAAGATATCGCCCTTCTCAAGTTCCATGGATGGGACCTGCCTCTTGAATGTGCTCGTACACTTCGCATTTCAACCATGCTGTTGAAGAAAGGGGCAAAGAGAGGGCTCACGCCATTCGACATCGGTAGCTTTATGTGTAGAGAAACCTTGATGAAGAAATCAATGATCGAGGAGTGCGTGGAAGAAGCAATGGATTCTTTGCTTCCTGGTACGAGTGAAGCAACATTCCTTGAAAGTGTATATGAAATAATGGATCACCGCCTTGATCAGATTGCTGCTTCATTGTCCTGATGGGGAAAGCTAAGATCATTTTAGTAACTAAATAATAGGTGTAATTAATTTATTAAAATATGTGGATAATGCATATAATATGATGATGTTAATGAATTATTATTATTATCATGTACGAACGACAGGACTATATCTGTTCTGCTTCTTCGTTCTTTGTTATAGCCGTTGAGAAGGATCGTCGGCGGCTTTGTCGATGGCATTTCTTTGGAATTGCTGTTCTTATTTATTATGTTTGTATTTGCCCTCCTAAGGTCTTTTTCCTTTGGATTTTCAATGGAAATTTAATAAATATTTTATGATTTTTTTTTAATATATATTTCCCTGA

Coding sequence (CDS)

ATGTCGTCTACGGGTGTTACTGCTCTTTGTCCAGTTCATAATGAGCATTTATTTTCCCATAATCGTTTAATTACTCCATCCATTCTGGAATCCAATGAGTCGATTTGGATTTATGTTTCTGTTTCGGGCTCTATGAGTCCTATGCCCATCTTTGCATCGGAATCCATTGAATCTGTGAAGTTAAGGATCCAATCTTGCAAGGGATTTGTGGTGAAGAAACAGAAGTTAGTTTGTGGTGGCCGAGAATTGTCTCGAAATAATTCATTAGTTCGGGATTATGGTGTTACGGATGGAAATGTGTTGCATTTGGTGCTTAGACTATCAGATCTTCAGGTTATTAATGTCAAGACTCATTGTGGGAAAGAGTTTACCTTCCATGTGGAGCGGGACAGAGATATTGCATATGTAAAAGAGAAGATTGCTACAAAGGTAAAGGAGTTTGTGGACGTTGATGACCATGAAGTAGTGTGTGATGGAAAGCCGCTAGATGATCATAGCCTTGTAGACGATATTTGTAATAGGCAGGATGCAGTCATACATTTGTTTGTTCGGAAGTCTGCAAAAGTACGAGGCAGACCTATCGAAAAAAACTTTGAGCTCTCTATTGTGGCATCAAATTTAAAAGAACAGTGTAAATCTGAGTTTTCTAGAACAAATAATCAGAAGGAATACAATGAGGATAAGGAAGCTTATAGGACAAAGTATGAATATGACAAAGAAATTGGACCAAGAAGGCCTTCTCCTAATGGAGGTAGTGTTATGGAGCCCATTGTCATTAACCCTAAGATTGAATTGCCAATACCCATTTGGGATATGGTCAATGCTACTGTAGATGGCTTAGACCGTGGGCATTTTCCAGTTAGGTCTTTGGAGGGAACCGGTGGAGCATATCTTATGTTAGACCCATCAGGAAAGAAGTATGTGTCTGTTTTTAAGCCAATTGATGAGGAGCCAATGGCCTTGAACAATCCACGGGGACTTCCATTGTCACTAGACGGTGAAGGGTTAAAGAAAGGAACCAAAGTTGGAGAAGGAGCATTCAGAGAAGTTGCAGCTTACCTATTAGATCATCCAATAAGTGGACGCCGTTCAATGTTCGGTGACAAGAATGGCTTTGCCGGTGTTCCTCCTACAGCATTGGTCAAGTGCCTTCATGACGGTTTCAACCATCCTAGTGATAAATCAGTTAAAATTGGTTCCTTGCAGATGTTCATGGAAAATAATGGTAGTTGTGAGGACTTTGGTGCTGGGGCTTTCCCTACCAAGGAGGTGCACAAAATCTCTGTTTTGGATATTAGATTGGCAAATGCAGATAGACATGCTGGAAATATTCTATTGGGAAAAGAAAGAGAAGGGGGTCAAACTGTGCTTATTCCAATCGACCATGGATACTGCTTGCCAACAAGTTTTGAAGATTGTACATTTGACTGGCTCTATTGGCCACAAGCCCAGCAACCATATGATGCTGAGACATTAGACTACATAAATTCACTGGACGCAGAGGAAGATATCGCCCTTCTCAAGTTCCATGGATGGGACCTGCCTCTTGAATGTGCTCGTACACTTCGCATTTCAACCATGCTGTTGAAGAAAGGGGCAAAGAGAGGGCTCACGCCATTCGACATCGGTAGCTTTATGTGTAGAGAAACCTTGATGAAGAAATCAATGATCGAGGAGTGCGTGGAAGAAGCAATGGATTCTTTGCTTCCTGGTACGAGTGAAGCAACATTCCTTGAAAGTGTATATGAAATAATGGATCACCGCCTTGATCAGATTGCTGCTTCATTGTCCTGA

Protein sequence

MSSTGVTALCPVHNEHLFSHNRLITPSILESNESIWIYVSVSGSMSPMPIFASESIESVKLRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTHCGKEFTFHVERDRDIAYVKEKIATKVKEFVDVDDHEVVCDGKPLDDHSLVDDICNRQDAVIHLFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNEDKEAYRTKYEYDKEIGPRRPSPNGGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYLMLDPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPISGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAFPTKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLYWPQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTPFDIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLDQIAASLS
Homology
BLAST of Bhi12M002069 vs. TAIR 10
Match: AT2G46500.1 (phosphoinositide 4-kinase gamma 4 )

HSP 1 Score: 718.0 bits (1852), Expect = 6.3e-207
Identity = 362/592 (61.15%), Postives = 453/592 (76.52%), Query Frame = 0

Query: 1   MSSTGVTALCPVHNEHLFSHNRLITPSILES--NESIWIYVSVSGSMSPMPIFASESIES 60
           MSS GV AL PV +E L     +   S L+S  +++I IY+++ GS+ PM +  S+SIES
Sbjct: 1   MSSAGV-ALSPVRSEPLIM-PLVRANSCLDSYPDDTIMIYLTLPGSVIPMRVLESDSIES 60

Query: 61  VKLRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTH 120
           VKLRIQS +GFVV+ QKLV GGREL+R+NS +RDYGV++GN+LHLVL+LSDLQV++VKT 
Sbjct: 61  VKLRIQSYRGFVVRNQKLVFGGRELARSNSNMRDYGVSEGNILHLVLKLSDLQVLDVKTT 120

Query: 121 CGKEFTFHVERDRDIAYVKEKIATKVKEFVDVDDHEVVCDGKPLDDHSLVDDICNRQDAV 180
           CGK   FHVER R+I YVK++I+ K  +FVD D+ E++ +G+ L+D SL++DIC   D+V
Sbjct: 121 CGKHCRFHVERGRNIGYVKKQISKKRGDFVDPDEQEILYEGEKLEDQSLINDICRNDDSV 180

Query: 181 IHLFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNEDKEAYRTKYEYD 240
           +HL VR+SAKVR +P+EKNFELSIVA   K                  DK+    K    
Sbjct: 181 LHLLVRRSAKVRVKPVEKNFELSIVAPQAK------------------DKKGREAK---- 240

Query: 241 KEIGPRRPSPNGGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYL 300
             + P++ S      +EP+V+N K ++P+ + DM+ +  DGL  G+ PVRS EGTGGAY 
Sbjct: 241 SIVPPKKLS------LEPVVVNSKAKVPLVVKDMIQSASDGLKSGNSPVRSSEGTGGAYF 300

Query: 301 MLDPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHP 360
           M  PSG K+V VFKPIDEEPMA NNP+GLPLS +GEGLKKGTKVGEGA REVAAY+LDHP
Sbjct: 301 MQGPSGNKFVGVFKPIDEEPMAENNPQGLPLSPNGEGLKKGTKVGEGALREVAAYILDHP 360

Query: 361 ISGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGA 420
            SG +SMFG++ GFAGVPPTA+++CLH GFNHP     KIGSLQMF EN+GSCED G  +
Sbjct: 361 KSGNKSMFGEEIGFAGVPPTAMIECLHPGFNHPKGIKTKIGSLQMFTENDGSCEDMGPLS 420

Query: 421 FPTKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWL 480
           FP +EVHKISVLDIRLANADRH GNIL+ K+ E G+ VL+PIDHGYCLP SFEDCTF+WL
Sbjct: 421 FPVEEVHKISVLDIRLANADRHGGNILMTKD-ESGKLVLVPIDHGYCLPESFEDCTFEWL 480

Query: 481 YWPQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLT 540
           YWPQA++PY AET +YI SLDAEEDI LLKFHGW +P E A+TLRISTMLLKKG +RGLT
Sbjct: 481 YWPQARKPYSAETQEYIRSLDAEEDIDLLKFHGWKMPAETAQTLRISTMLLKKGVERGLT 540

Query: 541 PFDIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLD 591
            F+IG+ MCRETL KKS++EE VEEA +++LPGTSEA FLE++ ++MD+ LD
Sbjct: 541 AFEIGTIMCRETLSKKSLVEEMVEEAQEAVLPGTSEAAFLEALSDVMDYHLD 561

BLAST of Bhi12M002069 vs. TAIR 10
Match: AT2G46500.2 (phosphoinositide 4-kinase gamma 4 )

HSP 1 Score: 718.0 bits (1852), Expect = 6.3e-207
Identity = 362/592 (61.15%), Postives = 453/592 (76.52%), Query Frame = 0

Query: 1   MSSTGVTALCPVHNEHLFSHNRLITPSILES--NESIWIYVSVSGSMSPMPIFASESIES 60
           MSS GV AL PV +E L     +   S L+S  +++I IY+++ GS+ PM +  S+SIES
Sbjct: 1   MSSAGV-ALSPVRSEPLIM-PLVRANSCLDSYPDDTIMIYLTLPGSVIPMRVLESDSIES 60

Query: 61  VKLRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTH 120
           VKLRIQS +GFVV+ QKLV GGREL+R+NS +RDYGV++GN+LHLVL+LSDLQV++VKT 
Sbjct: 61  VKLRIQSYRGFVVRNQKLVFGGRELARSNSNMRDYGVSEGNILHLVLKLSDLQVLDVKTT 120

Query: 121 CGKEFTFHVERDRDIAYVKEKIATKVKEFVDVDDHEVVCDGKPLDDHSLVDDICNRQDAV 180
           CGK   FHVER R+I YVK++I+ K  +FVD D+ E++ +G+ L+D SL++DIC   D+V
Sbjct: 121 CGKHCRFHVERGRNIGYVKKQISKKRGDFVDPDEQEILYEGEKLEDQSLINDICRNDDSV 180

Query: 181 IHLFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNEDKEAYRTKYEYD 240
           +HL VR+SAKVR +P+EKNFELSIVA   K                  DK+    K    
Sbjct: 181 LHLLVRRSAKVRVKPVEKNFELSIVAPQAK------------------DKKGREAK---- 240

Query: 241 KEIGPRRPSPNGGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYL 300
             + P++ S      +EP+V+N K ++P+ + DM+ +  DGL  G+ PVRS EGTGGAY 
Sbjct: 241 SIVPPKKLS------LEPVVVNSKAKVPLVVKDMIQSASDGLKSGNSPVRSSEGTGGAYF 300

Query: 301 MLDPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHP 360
           M  PSG K+V VFKPIDEEPMA NNP+GLPLS +GEGLKKGTKVGEGA REVAAY+LDHP
Sbjct: 301 MQGPSGNKFVGVFKPIDEEPMAENNPQGLPLSPNGEGLKKGTKVGEGALREVAAYILDHP 360

Query: 361 ISGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGA 420
            SG +SMFG++ GFAGVPPTA+++CLH GFNHP     KIGSLQMF EN+GSCED G  +
Sbjct: 361 KSGNKSMFGEEIGFAGVPPTAMIECLHPGFNHPKGIKTKIGSLQMFTENDGSCEDMGPLS 420

Query: 421 FPTKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWL 480
           FP +EVHKISVLDIRLANADRH GNIL+ K+ E G+ VL+PIDHGYCLP SFEDCTF+WL
Sbjct: 421 FPVEEVHKISVLDIRLANADRHGGNILMTKD-ESGKLVLVPIDHGYCLPESFEDCTFEWL 480

Query: 481 YWPQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLT 540
           YWPQA++PY AET +YI SLDAEEDI LLKFHGW +P E A+TLRISTMLLKKG +RGLT
Sbjct: 481 YWPQARKPYSAETQEYIRSLDAEEDIDLLKFHGWKMPAETAQTLRISTMLLKKGVERGLT 540

Query: 541 PFDIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLD 591
            F+IG+ MCRETL KKS++EE VEEA +++LPGTSEA FLE++ ++MD+ LD
Sbjct: 541 AFEIGTIMCRETLSKKSLVEEMVEEAQEAVLPGTSEAAFLEALSDVMDYHLD 561

BLAST of Bhi12M002069 vs. TAIR 10
Match: AT5G24240.1 (Phosphatidylinositol 3- and 4-kinase ;Ubiquitin family protein )

HSP 1 Score: 642.5 bits (1656), Expect = 3.4e-184
Identity = 316/567 (55.73%), Postives = 412/567 (72.66%), Query Frame = 0

Query: 32  NESIWIYVSVSGSMSPMPIFASESIESVKLRIQSCKGFVVKKQKLVCGGRELSRNNSLVR 91
           ++ I ++++++GS+ P  +  S+SI SVKLRIQS KGF VKKQKL+  GRE+SRN+S +R
Sbjct: 30  DDPILVFLTIAGSVIPKRVMESDSIASVKLRIQSIKGFFVKKQKLLYDGREVSRNDSQIR 89

Query: 92  DYGVTDGNVLHLVLRLSDLQVINVKTHCGKEFTFHVERDRDIAYVKEKIATKVKEFVDVD 151
           DYG+ DG +LHLV+RLSDLQ I+V+T  GKEF   VER R++ YVK++IA+K KE     
Sbjct: 90  DYGLADGKLLHLVIRLSDLQAISVRTVDGKEFELVVERSRNVGYVKQQIASKEKELGIPR 149

Query: 152 DHEVVCDGKPLDDHSLVDDICNRQDAVIHLFVRKSAKVRGRPIEKNFELSIVASNLKEQC 211
           DHE+  DG+ LDD  L+ D+C   D VIHL + KSAKVR +P+ K+FE+ I   N K   
Sbjct: 150 DHELTLDGEELDDQRLITDLCQNGDNVIHLLISKSAKVRAKPVGKDFEVFIEDVNHKHNV 209

Query: 212 KSEFSRTNNQKEYNEDKEAYRTKYEYDKEIGPRRPSPNGGSVMEPIVINPKIELPIPIWD 271
                R  N     + KE +                      +EP ++NP+I+LPI + +
Sbjct: 210 DGR--RGKNISSEAKPKEFF----------------------VEPFIVNPEIKLPILLKE 269

Query: 272 MVNATVDGLDRGHFPVRSLEGTGGAYLMLDPSGKKYVSVFKPIDEEPMALNNPRGLPLSL 331
           ++++T++GL++G+ P+RS +G+GGAY M DPSG KYVSVFKPIDEEPMA+NNP G P+S+
Sbjct: 270 LISSTLEGLEKGNGPIRSSDGSGGAYFMQDPSGHKYVSVFKPIDEEPMAVNNPHGQPVSV 329

Query: 332 DGEGLKKGTKVGEGAFREVAAYLLDHPISGRRSMFGDKNGFAGVPPTALVKCLHDGFNHP 391
           DGEGLKKGT+VGEGA REVAAY+LD+P++G R+   D+ GFAGVPPT +VKCLH  FNHP
Sbjct: 330 DGEGLKKGTQVGEGAIREVAAYILDYPMTGPRTFPHDQTGFAGVPPTTMVKCLHKDFNHP 389

Query: 392 -----SDKSVKIGSLQMFMENNGSCEDFGAGAFPTKEVHKISVLDIRLANADRHAGNILL 451
                S ++ KIGSLQMF+ N GSCED G   FP  +VHKISVLDIRLANADRHAGNIL+
Sbjct: 390 NGYSFSPENTKIGSLQMFVSNVGSCEDMGYRVFPVDQVHKISVLDIRLANADRHAGNILV 449

Query: 452 GKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLYWPQAQQPYDAETLDYINSLDAEEDIAL 511
            ++ + GQ VL PIDHGYC P  FEDCTF+WLYWPQA++PY +ETL+YI SLD E+DI L
Sbjct: 450 SRDGKDGQMVLTPIDHGYCFPNKFEDCTFEWLYWPQAKEPYSSETLEYIKSLDPEKDIEL 509

Query: 512 LKFHGWDLPLECARTLRISTMLLKKGAKRGLTPFDIGSFMCRETLMKKSMIEECVEEAMD 571
           L+FHGW++P  C R LRISTMLLKKG+ +GLTPF IGS MCRETL ++S+IE+ + +A  
Sbjct: 510 LRFHGWEIPPSCTRVLRISTMLLKKGSAKGLTPFTIGSIMCRETLKEESVIEQIIHDAEA 569

Query: 572 SLLPGTSEATFLESVYEIMDHRLDQIA 594
            +   T+E  F+ +V  IMD+RLDQ A
Sbjct: 570 IVPTETTEDEFISTVSAIMDNRLDQYA 572

BLAST of Bhi12M002069 vs. TAIR 10
Match: AT2G46500.3 (phosphoinositide 4-kinase gamma 4 )

HSP 1 Score: 457.2 bits (1175), Expect = 2.0e-128
Identity = 240/423 (56.74%), Postives = 307/423 (72.58%), Query Frame = 0

Query: 1   MSSTGVTALCPVHNEHLFSHNRLITPSILES--NESIWIYVSVSGSMSPMPIFASESIES 60
           MSS GV AL PV +E L     +   S L+S  +++I IY+++ GS+ PM +  S+SIES
Sbjct: 1   MSSAGV-ALSPVRSEPLIM-PLVRANSCLDSYPDDTIMIYLTLPGSVIPMRVLESDSIES 60

Query: 61  VKLRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTH 120
           VKLRIQS +GFVV+ QKLV GGREL+R+NS +RDYGV++GN+LHLVL+LSDLQV++VKT 
Sbjct: 61  VKLRIQSYRGFVVRNQKLVFGGRELARSNSNMRDYGVSEGNILHLVLKLSDLQVLDVKTT 120

Query: 121 CGKEFTFHVERDRDIAYVKEKIATKVKEFVDVDDHEVVCDGKPLDDHSLVDDICNRQDAV 180
           CGK   FHVER R+I YVK++I+ K  +FVD D+ E++ +G+ L+D SL++DIC   D+V
Sbjct: 121 CGKHCRFHVERGRNIGYVKKQISKKRGDFVDPDEQEILYEGEKLEDQSLINDICRNDDSV 180

Query: 181 IHLFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNEDKEAYRTKYEYD 240
           +HL VR+SAKVR +P+EKNFELSIVA   K                  DK+    K    
Sbjct: 181 LHLLVRRSAKVRVKPVEKNFELSIVAPQAK------------------DKKGREAK---- 240

Query: 241 KEIGPRRPSPNGGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYL 300
             + P++ S      +EP+V+N K ++P+ + DM+ +  DGL  G+ PVRS EGTGGAY 
Sbjct: 241 SIVPPKKLS------LEPVVVNSKAKVPLVVKDMIQSASDGLKSGNSPVRSSEGTGGAYF 300

Query: 301 MLDPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHP 360
           M  PSG K+V VFKPIDEEPMA NNP+GLPLS +GEGLKKGTKVGEGA REVAAY+LDHP
Sbjct: 301 MQGPSGNKFVGVFKPIDEEPMAENNPQGLPLSPNGEGLKKGTKVGEGALREVAAYILDHP 360

Query: 361 ISGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGA 420
            SG +SMFG++ GFAGVPPTA+++CLH GFNHP     KIGSLQMF EN+GSCED G  +
Sbjct: 361 KSGNKSMFGEEIGFAGVPPTAMIECLHPGFNHPKGIKTKIGSLQMFTENDGSCEDMGPLS 393

Query: 421 FPT 422
           FPT
Sbjct: 421 FPT 393

BLAST of Bhi12M002069 vs. TAIR 10
Match: AT1G64460.1 (Protein kinase superfamily protein )

HSP 1 Score: 424.9 bits (1091), Expect = 1.1e-118
Identity = 202/300 (67.33%), Postives = 243/300 (81.00%), Query Frame = 0

Query: 299 MLDPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHP 358
           M D SG  YVSVFKP+DEEPMA+NNP+ LP+S DG+GLK+GT+VGEGA REVAAYLLDHP
Sbjct: 1   MQDSSGLNYVSVFKPMDEEPMAVNNPQQLPVSSDGQGLKRGTRVGEGATREVAAYLLDHP 60

Query: 359 ISGRRSMFGDKNGFAGVPPTALVKCLHDGFNHP------SDKSVKIGSLQMFMENNGSCE 418
            SG RS+  +  GFAGVPPTA+V+  H  +N+P      + K  K+GSLQMFM+NNGSCE
Sbjct: 61  KSGLRSVSKEVMGFAGVPPTAMVRSSHKVYNYPNGFSSCATKDAKVGSLQMFMKNNGSCE 120

Query: 419 DFGAGAFPTKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFED 478
           D G GAFP +EVHKI V DIR+ANADRHAGNIL GK  E G+T+LIPIDHGYCLP +FED
Sbjct: 121 DIGPGAFPVEEVHKICVFDIRMANADRHAGNILTGKSEE-GKTLLIPIDHGYCLPENFED 180

Query: 479 CTFDWLYWPQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKG 538
           CTF+WLYWPQA+ P+ A+T+DYINSLD+E+DIALL+ HGW++P   +RTLRISTMLLKKG
Sbjct: 181 CTFEWLYWPQAKLPFSADTIDYINSLDSEQDIALLQLHGWNVPEAVSRTLRISTMLLKKG 240

Query: 539 AKRGLTPFDIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLDQI 593
            +R LTP+ IGS MCRET+ K S IEE V EA +S+LP +SEATFLE+V   MD RLD++
Sbjct: 241 VERNLTPYQIGSVMCRETVNKDSAIEEIVREAHNSVLPASSEATFLEAVSVAMDRRLDEL 299

BLAST of Bhi12M002069 vs. ExPASy Swiss-Prot
Match: Q9ZPY9 (Phosphatidylinositol 4-kinase gamma 4 OS=Arabidopsis thaliana OX=3702 GN=PI4KG4 PE=1 SV=1)

HSP 1 Score: 718.0 bits (1852), Expect = 8.8e-206
Identity = 362/592 (61.15%), Postives = 453/592 (76.52%), Query Frame = 0

Query: 1   MSSTGVTALCPVHNEHLFSHNRLITPSILES--NESIWIYVSVSGSMSPMPIFASESIES 60
           MSS GV AL PV +E L     +   S L+S  +++I IY+++ GS+ PM +  S+SIES
Sbjct: 1   MSSAGV-ALSPVRSEPLIM-PLVRANSCLDSYPDDTIMIYLTLPGSVIPMRVLESDSIES 60

Query: 61  VKLRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTH 120
           VKLRIQS +GFVV+ QKLV GGREL+R+NS +RDYGV++GN+LHLVL+LSDLQV++VKT 
Sbjct: 61  VKLRIQSYRGFVVRNQKLVFGGRELARSNSNMRDYGVSEGNILHLVLKLSDLQVLDVKTT 120

Query: 121 CGKEFTFHVERDRDIAYVKEKIATKVKEFVDVDDHEVVCDGKPLDDHSLVDDICNRQDAV 180
           CGK   FHVER R+I YVK++I+ K  +FVD D+ E++ +G+ L+D SL++DIC   D+V
Sbjct: 121 CGKHCRFHVERGRNIGYVKKQISKKRGDFVDPDEQEILYEGEKLEDQSLINDICRNDDSV 180

Query: 181 IHLFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNEDKEAYRTKYEYD 240
           +HL VR+SAKVR +P+EKNFELSIVA   K                  DK+    K    
Sbjct: 181 LHLLVRRSAKVRVKPVEKNFELSIVAPQAK------------------DKKGREAK---- 240

Query: 241 KEIGPRRPSPNGGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYL 300
             + P++ S      +EP+V+N K ++P+ + DM+ +  DGL  G+ PVRS EGTGGAY 
Sbjct: 241 SIVPPKKLS------LEPVVVNSKAKVPLVVKDMIQSASDGLKSGNSPVRSSEGTGGAYF 300

Query: 301 MLDPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHP 360
           M  PSG K+V VFKPIDEEPMA NNP+GLPLS +GEGLKKGTKVGEGA REVAAY+LDHP
Sbjct: 301 MQGPSGNKFVGVFKPIDEEPMAENNPQGLPLSPNGEGLKKGTKVGEGALREVAAYILDHP 360

Query: 361 ISGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGA 420
            SG +SMFG++ GFAGVPPTA+++CLH GFNHP     KIGSLQMF EN+GSCED G  +
Sbjct: 361 KSGNKSMFGEEIGFAGVPPTAMIECLHPGFNHPKGIKTKIGSLQMFTENDGSCEDMGPLS 420

Query: 421 FPTKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWL 480
           FP +EVHKISVLDIRLANADRH GNIL+ K+ E G+ VL+PIDHGYCLP SFEDCTF+WL
Sbjct: 421 FPVEEVHKISVLDIRLANADRHGGNILMTKD-ESGKLVLVPIDHGYCLPESFEDCTFEWL 480

Query: 481 YWPQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLT 540
           YWPQA++PY AET +YI SLDAEEDI LLKFHGW +P E A+TLRISTMLLKKG +RGLT
Sbjct: 481 YWPQARKPYSAETQEYIRSLDAEEDIDLLKFHGWKMPAETAQTLRISTMLLKKGVERGLT 540

Query: 541 PFDIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLD 591
            F+IG+ MCRETL KKS++EE VEEA +++LPGTSEA FLE++ ++MD+ LD
Sbjct: 541 AFEIGTIMCRETLSKKSLVEEMVEEAQEAVLPGTSEAAFLEALSDVMDYHLD 561

BLAST of Bhi12M002069 vs. ExPASy Swiss-Prot
Match: Q9FNF8 (Phosphatidylinositol 4-kinase gamma 3 OS=Arabidopsis thaliana OX=3702 GN=PI4KG3 PE=2 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 4.7e-183
Identity = 316/567 (55.73%), Postives = 412/567 (72.66%), Query Frame = 0

Query: 32  NESIWIYVSVSGSMSPMPIFASESIESVKLRIQSCKGFVVKKQKLVCGGRELSRNNSLVR 91
           ++ I ++++++GS+ P  +  S+SI SVKLRIQS KGF VKKQKL+  GRE+SRN+S +R
Sbjct: 30  DDPILVFLTIAGSVIPKRVMESDSIASVKLRIQSIKGFFVKKQKLLYDGREVSRNDSQIR 89

Query: 92  DYGVTDGNVLHLVLRLSDLQVINVKTHCGKEFTFHVERDRDIAYVKEKIATKVKEFVDVD 151
           DYG+ DG +LHLV+RLSDLQ I+V+T  GKEF   VER R++ YVK++IA+K KE     
Sbjct: 90  DYGLADGKLLHLVIRLSDLQAISVRTVDGKEFELVVERSRNVGYVKQQIASKEKELGIPR 149

Query: 152 DHEVVCDGKPLDDHSLVDDICNRQDAVIHLFVRKSAKVRGRPIEKNFELSIVASNLKEQC 211
           DHE+  DG+ LDD  L+ D+C   D VIHL + KSAKVR +P+ K+FE+ I   N K   
Sbjct: 150 DHELTLDGEELDDQRLITDLCQNGDNVIHLLISKSAKVRAKPVGKDFEVFIEDVNHKHNV 209

Query: 212 KSEFSRTNNQKEYNEDKEAYRTKYEYDKEIGPRRPSPNGGSVMEPIVINPKIELPIPIWD 271
                R  N     + KE +                      +EP ++NP+I+LPI + +
Sbjct: 210 DGR--RGKNISSEAKPKEFF----------------------VEPFIVNPEIKLPILLKE 269

Query: 272 MVNATVDGLDRGHFPVRSLEGTGGAYLMLDPSGKKYVSVFKPIDEEPMALNNPRGLPLSL 331
           ++++T++GL++G+ P+RS +G+GGAY M DPSG KYVSVFKPIDEEPMA+NNP G P+S+
Sbjct: 270 LISSTLEGLEKGNGPIRSSDGSGGAYFMQDPSGHKYVSVFKPIDEEPMAVNNPHGQPVSV 329

Query: 332 DGEGLKKGTKVGEGAFREVAAYLLDHPISGRRSMFGDKNGFAGVPPTALVKCLHDGFNHP 391
           DGEGLKKGT+VGEGA REVAAY+LD+P++G R+   D+ GFAGVPPT +VKCLH  FNHP
Sbjct: 330 DGEGLKKGTQVGEGAIREVAAYILDYPMTGPRTFPHDQTGFAGVPPTTMVKCLHKDFNHP 389

Query: 392 -----SDKSVKIGSLQMFMENNGSCEDFGAGAFPTKEVHKISVLDIRLANADRHAGNILL 451
                S ++ KIGSLQMF+ N GSCED G   FP  +VHKISVLDIRLANADRHAGNIL+
Sbjct: 390 NGYSFSPENTKIGSLQMFVSNVGSCEDMGYRVFPVDQVHKISVLDIRLANADRHAGNILV 449

Query: 452 GKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLYWPQAQQPYDAETLDYINSLDAEEDIAL 511
            ++ + GQ VL PIDHGYC P  FEDCTF+WLYWPQA++PY +ETL+YI SLD E+DI L
Sbjct: 450 SRDGKDGQMVLTPIDHGYCFPNKFEDCTFEWLYWPQAKEPYSSETLEYIKSLDPEKDIEL 509

Query: 512 LKFHGWDLPLECARTLRISTMLLKKGAKRGLTPFDIGSFMCRETLMKKSMIEECVEEAMD 571
           L+FHGW++P  C R LRISTMLLKKG+ +GLTPF IGS MCRETL ++S+IE+ + +A  
Sbjct: 510 LRFHGWEIPPSCTRVLRISTMLLKKGSAKGLTPFTIGSIMCRETLKEESVIEQIIHDAEA 569

Query: 572 SLLPGTSEATFLESVYEIMDHRLDQIA 594
            +   T+E  F+ +V  IMD+RLDQ A
Sbjct: 570 IVPTETTEDEFISTVSAIMDNRLDQYA 572

BLAST of Bhi12M002069 vs. ExPASy Swiss-Prot
Match: Q9SGW8 (Phosphatidylinositol 4-kinase gamma 2 OS=Arabidopsis thaliana OX=3702 GN=PI4KG2 PE=1 SV=2)

HSP 1 Score: 624.0 bits (1608), Expect = 1.7e-177
Identity = 324/597 (54.27%), Postives = 412/597 (69.01%), Query Frame = 0

Query: 3   STGVTALCPVHNEHLFSHNRLITPSILE-SNESIWIYVSVSGSMSPMPIFASESIESVKL 62
           S    AL P+H    F+       +    S +S+ +++SVSGS  PM I  S+SI  VKL
Sbjct: 2   SVADVALSPIHRGSAFAVGGFGQSTTTHYSVKSVLVFLSVSGSTMPMLILESDSIAEVKL 61

Query: 63  RIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTHCGK 122
           RIQ+C GF V++QKLV  GREL+RN S V+DYGVT G+VLHLVL+L D  ++ V T CGK
Sbjct: 62  RIQTCNGFRVRRQKLVFSGRELARNASRVKDYGVTGGSVLHLVLKLYDPLLVTVITTCGK 121

Query: 123 EFTFHVERDRDIAYVKEKIATKVKEFVDVDDHEVVCDGKPLDDHSLVDDICNRQDAVIHL 182
            F FHV+R R++ Y+K++I+ + K F +VDD E++  G+ LDD+ ++D IC   ++VIHL
Sbjct: 122 VFQFHVDRRRNVGYLKKRISKEGKGFPEVDDQEILFKGEKLDDNRIIDGICKDGNSVIHL 181

Query: 183 FVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNEDKEAYRTKYEYDKEI 242
            V+KS                                       ED      K E D   
Sbjct: 182 LVKKSV--------------------------------------ED----TVKREEDTAT 241

Query: 243 GPRRPSPNGGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYLMLD 302
           G         S++EP+V+NP ++LP  + DM++ TVDGL++G  PVRS EGTGG YLM D
Sbjct: 242 GK-------DSLLEPVVLNPDVKLPEVLEDMIDRTVDGLNKGSPPVRSAEGTGGTYLMQD 301

Query: 303 PSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPISG 362
            SG  YVSVFKP+DEEPMA+NNP+ LP+S DG+GLK+GT+VGEGA REVAAYLLDHP SG
Sbjct: 302 SSGLNYVSVFKPMDEEPMAVNNPQQLPVSSDGQGLKRGTRVGEGATREVAAYLLDHPKSG 361

Query: 363 RRSMFGDKNGFAGVPPTALVKCLHDGFNHP------SDKSVKIGSLQMFMENNGSCEDFG 422
            RS+  +  GFAGVPPTA+V+  H  +N+P      + K  K+GSLQMFM+NNGSCED G
Sbjct: 362 LRSVSKEVMGFAGVPPTAMVRSSHKVYNYPNGFSSCATKDAKVGSLQMFMKNNGSCEDIG 421

Query: 423 AGAFPTKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTF 482
            GAFP +EVHKI V DIR+ANADRHAGNIL GK  E G+T+LIPIDHGYCLP +FEDCTF
Sbjct: 422 PGAFPVEEVHKICVFDIRMANADRHAGNILTGKSEE-GKTLLIPIDHGYCLPENFEDCTF 481

Query: 483 DWLYWPQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKR 542
           +WLYWPQA+ P+ A+T+DYINSLD+E+DIALL+ HGW++P   +RTLRISTMLLKKG +R
Sbjct: 482 EWLYWPQAKLPFSADTIDYINSLDSEQDIALLQLHGWNVPEAVSRTLRISTMLLKKGVER 541

Query: 543 GLTPFDIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLDQI 593
            LTP+ IGS MCRET+ K S IEE V EA +S+LP +SEATFLE+V   MD RLD++
Sbjct: 542 NLTPYQIGSVMCRETVNKDSAIEEIVREAHNSVLPASSEATFLEAVSVAMDRRLDEL 548

BLAST of Bhi12M002069 vs. ExPASy Swiss-Prot
Match: Q6K881 (Phosphatidylinositol 4-kinase gamma 4 OS=Oryza sativa subsp. japonica OX=39947 GN=PI4KG4 PE=1 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 6.0e-170
Identity = 309/598 (51.67%), Postives = 415/598 (69.40%), Query Frame = 0

Query: 1   MSSTGVTALCPVHNEHLFSHNRLITPSILESNESIWIYVSVSG-SMSPMPIFASESIESV 60
           MSS G+  L P+ ++  F+ +       L+  +SI I++++ G +  PM +  S+S+ SV
Sbjct: 1   MSSAGIATLSPLLDQFCFAPH---GEPRLQQLDSIVIFLAMPGVAPMPMRVLHSDSVASV 60

Query: 61  KLRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTHC 120
           KLRIQ  KGFV  KQ+LV  G ELS NNS VRDYG+TDGNVLHLV+RL+DL+ I+++T  
Sbjct: 61  KLRIQQFKGFVTTKQRLVFSGHELSLNNSHVRDYGLTDGNVLHLVVRLADLRAISIETAN 120

Query: 121 GKEFTFHVERDRDIAYVKEKI-ATKVKEFVDVDDHEVVCDGKPLDDHSLVDDICNRQDAV 180
           GK+F F VE   ++ Y+K+K+ A   ++   + D  +V DG+ L+D+ L+ DI  +  AV
Sbjct: 121 GKKFQFQVESCCNVGYLKDKLSAESGQQLGSLKDQRLVFDGEELEDNQLIADISKKGAAV 180

Query: 181 IHLFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNEDKEAYRTKYEYD 240
           IHLF+R+ AKV+ +  +K   +++V     +  +++                        
Sbjct: 181 IHLFIRRPAKVQTQQGDKETVVTVVTPKDNDNLQTD------------------------ 240

Query: 241 KEIGPRRPSPNGGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYL 300
             +   +P+    + +EPI+ N K++L   + +M+ +T+ G++ G+ PV S EG+GG Y 
Sbjct: 241 -ALNLAKPAKGKPAPVEPIIANGKVKLSPAVMEMIYSTISGIENGYLPVMSTEGSGGVYF 300

Query: 301 MLDPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHP 360
           M D SG+  V+VFKPIDEEPMA NNPRGLPLS DGEGLK+GT+VGEGA REVAAY+LDHP
Sbjct: 301 MKDSSGESNVAVFKPIDEEPMAKNNPRGLPLSTDGEGLKRGTRVGEGALREVAAYILDHP 360

Query: 361 ISGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGA 420
           + G +S   D  GF+GVPPTALV+C H G       S K+GSLQ+F++NNGSCED G  A
Sbjct: 361 VYGCKSC--DVPGFSGVPPTALVRCFHMG-----KGSNKVGSLQLFVDNNGSCEDMGPRA 420

Query: 421 FPTKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWL 480
           FP KEV KI++LDIRLANADRHAGNIL+ ++ E     LIPIDHGYCLP  FEDCTF+WL
Sbjct: 421 FPVKEVQKIAILDIRLANADRHAGNILVCQDGE-DHLKLIPIDHGYCLPEKFEDCTFEWL 480

Query: 481 YWPQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLT 540
           YWPQA++P+  ET  YI SLDA++DIALLKFHGW L  +CAR LRISTMLLKKGA+RGLT
Sbjct: 481 YWPQAREPFGPETAAYIGSLDADKDIALLKFHGWALSPQCARVLRISTMLLKKGAERGLT 540

Query: 541 PFDIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLDQIAASL 597
           P+DIGS +CR+T+ K+S IE  +EEA D++LPGTSE TFLE++ EIMD  LD++A  L
Sbjct: 541 PYDIGSILCRQTVKKESEIEAIIEEAEDAILPGTSEETFLETISEIMDFHLDKLAVKL 562

BLAST of Bhi12M002069 vs. ExPASy Swiss-Prot
Match: Q8W4R8 (Phosphatidylinositol 4-kinase gamma 6 OS=Arabidopsis thaliana OX=3702 GN=PI4KG6 PE=2 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 4.9e-55
Identity = 144/384 (37.50%), Postives = 190/384 (49.48%), Query Frame = 0

Query: 247 SPNGGSVME-----PIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYLMLD 306
           SP G  +       PI I       + +    N  V  +  G  P+    G GGAY   D
Sbjct: 117 SPTGNDLQRKDRSGPIEILSHSPCFLSLKQTANDIVKAMKMGVEPIPVNGGLGGAYYFRD 176

Query: 307 PSGKKYVSVFKPIDEEPMALNNPRG-LPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPIS 366
             G+  V++ KP DEEP A NNP+G +  +L   GLK   +VGE  FREVAAYLLD+   
Sbjct: 177 EKGQS-VAIVKPTDEEPFAPNNPKGFVGKALGQPGLKPSVRVGETGFREVAAYLLDY--- 236

Query: 367 GRRSMFGDKNGFAGVPPTALVKCLHDGF--------NHPSDK----SVKIGSLQMFMENN 426
                    + FA VPPTALVK  H  F        N   +K    S KI S Q F+ ++
Sbjct: 237 ---------DHFANVPPTALVKITHSVFNVNDGMDGNKSREKKKLVSSKIASFQKFVPHD 296

Query: 427 GSCEDFGAGAFPTKEVHKISVLDIRLANADRHAGNILLGKEREG-----GQTVLIPIDHG 486
               D G  +FP   VH+I +LDIR+ N DRH GN+L+ K  +G     GQ  LIPIDHG
Sbjct: 297 FDASDHGTSSFPVASVHRIGILDIRILNTDRHGGNLLVKKLDDGGVGRFGQVELIPIDHG 356

Query: 487 YCLPTSFEDCTFDWLYWPQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPL---ECAR 546
            CLP + ED  F+W++WPQA  P+  E LDYI SLD  +D  +L+    +LP+    C R
Sbjct: 357 LCLPETLEDPYFEWIHWPQASIPFSEEELDYIQSLDPVKDCEMLR---RELPMIREACLR 416

Query: 547 TLRISTMLLKKGAKRGLTPFDIGSFMCRETLMKKSMIEE----CVE-----EAMDSLLPG 596
            L + T+ LK+ A  GL   +IG  M RE    +    E    C+E        D L P 
Sbjct: 417 VLVLCTVFLKEAAVFGLCLAEIGEMMTREFRAGEEEPSELEMLCIEAKRLTTEQDVLSPK 476

BLAST of Bhi12M002069 vs. NCBI nr
Match: XP_038874462.1 (phosphatidylinositol 4-kinase gamma 4 [Benincasa hispida])

HSP 1 Score: 1219.1 bits (3153), Expect = 0.0e+00
Identity = 597/597 (100.00%), Postives = 597/597 (100.00%), Query Frame = 0

Query: 1   MSSTGVTALCPVHNEHLFSHNRLITPSILESNESIWIYVSVSGSMSPMPIFASESIESVK 60
           MSSTGVTALCPVHNEHLFSHNRLITPSILESNESIWIYVSVSGSMSPMPIFASESIESVK
Sbjct: 1   MSSTGVTALCPVHNEHLFSHNRLITPSILESNESIWIYVSVSGSMSPMPIFASESIESVK 60

Query: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTHCG 120
           LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTHCG
Sbjct: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTHCG 120

Query: 121 KEFTFHVERDRDIAYVKEKIATKVKEFVDVDDHEVVCDGKPLDDHSLVDDICNRQDAVIH 180
           KEFTFHVERDRDIAYVKEKIATKVKEFVDVDDHEVVCDGKPLDDHSLVDDICNRQDAVIH
Sbjct: 121 KEFTFHVERDRDIAYVKEKIATKVKEFVDVDDHEVVCDGKPLDDHSLVDDICNRQDAVIH 180

Query: 181 LFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNEDKEAYRTKYEYDKE 240
           LFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNEDKEAYRTKYEYDKE
Sbjct: 181 LFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNEDKEAYRTKYEYDKE 240

Query: 241 IGPRRPSPNGGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYLML 300
           IGPRRPSPNGGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYLML
Sbjct: 241 IGPRRPSPNGGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYLML 300

Query: 301 DPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPIS 360
           DPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPIS
Sbjct: 301 DPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPIS 360

Query: 361 GRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAFP 420
           GRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAFP
Sbjct: 361 GRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAFP 420

Query: 421 TKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLYW 480
           TKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLYW
Sbjct: 421 TKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLYW 480

Query: 481 PQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTPF 540
           PQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTPF
Sbjct: 481 PQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTPF 540

Query: 541 DIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLDQIAASLS 598
           DIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLDQIAASLS
Sbjct: 541 DIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLDQIAASLS 597

BLAST of Bhi12M002069 vs. NCBI nr
Match: XP_008459989.1 (PREDICTED: phosphatidylinositol 4-kinase gamma 4 [Cucumis melo])

HSP 1 Score: 1156.0 bits (2989), Expect = 0.0e+00
Identity = 565/598 (94.48%), Postives = 580/598 (96.99%), Query Frame = 0

Query: 1   MSSTGVTALCPVHNEHLFSHNRLITPSILESNESIWIYVSVSGSMSPMPIFASESIESVK 60
           MSSTGV ALCP  NEHL SH RLITPS L+SNESIWIYVSVSGSMSPMPIFASESIESVK
Sbjct: 1   MSSTGVIALCPARNEHLVSHKRLITPSALQSNESIWIYVSVSGSMSPMPIFASESIESVK 60

Query: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTHCG 120
           LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKT+CG
Sbjct: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTYCG 120

Query: 121 KEFTFHVERDRDIAYVKEKIATKVKEFVD-VDDHEVVCDGKPLDDHSLVDDICNRQDAVI 180
           KEFTFHVERDRD+AYVKEKIATKVK+FVD VD+HEVVCDGKPLDDHSLVDDICNRQDAVI
Sbjct: 121 KEFTFHVERDRDVAYVKEKIATKVKDFVDVVDEHEVVCDGKPLDDHSLVDDICNRQDAVI 180

Query: 181 HLFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNEDKEAYRTKYEYDK 240
           HLFVRKSAKVRG+P++KNFELSIVASN +EQCKSEFSR NNQK Y EDKEAYRTKYEYDK
Sbjct: 181 HLFVRKSAKVRGKPVDKNFELSIVASNFEEQCKSEFSRENNQKVYKEDKEAYRTKYEYDK 240

Query: 241 EIGPRRPSPNGGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYLM 300
           EI PRRP PNGGSV+EPIV+NPK+ELPIPIWDMVN+TVDGLD GHFPVRSLEGTGGAYLM
Sbjct: 241 EIAPRRPYPNGGSVLEPIVVNPKVELPIPIWDMVNSTVDGLDCGHFPVRSLEGTGGAYLM 300

Query: 301 LDPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPI 360
           LD SGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPI
Sbjct: 301 LDRSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPI 360

Query: 361 SGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF 420
           SGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF
Sbjct: 361 SGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF 420

Query: 421 PTKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLY 480
            TKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLY
Sbjct: 421 STKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLY 480

Query: 481 WPQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP 540
           WPQ+QQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP
Sbjct: 481 WPQSQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP 540

Query: 541 FDIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLDQIAASLS 598
           FDIGSFMCRETL KKSMIEECVEEAMDSL+PGTSEATFLESVYEIMD RLDQIAASLS
Sbjct: 541 FDIGSFMCRETLTKKSMIEECVEEAMDSLVPGTSEATFLESVYEIMDRRLDQIAASLS 598

BLAST of Bhi12M002069 vs. NCBI nr
Match: KAA0039904.1 (phosphatidylinositol 4-kinase gamma 4 [Cucumis melo var. makuwa] >TYK24596.1 phosphatidylinositol 4-kinase gamma 4 [Cucumis melo var. makuwa])

HSP 1 Score: 1154.4 bits (2985), Expect = 0.0e+00
Identity = 564/598 (94.31%), Postives = 579/598 (96.82%), Query Frame = 0

Query: 1   MSSTGVTALCPVHNEHLFSHNRLITPSILESNESIWIYVSVSGSMSPMPIFASESIESVK 60
           MSSTGV ALCP  NEHL SH RL TPS L+SNESIWIYVSVSGSMSPMPIFASESIESVK
Sbjct: 1   MSSTGVIALCPARNEHLVSHKRLFTPSALQSNESIWIYVSVSGSMSPMPIFASESIESVK 60

Query: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTHCG 120
           LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKT+CG
Sbjct: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTYCG 120

Query: 121 KEFTFHVERDRDIAYVKEKIATKVKEFVD-VDDHEVVCDGKPLDDHSLVDDICNRQDAVI 180
           KEFTFHVERDRD+AYVKEKIATKVK+FVD VD+HEVVCDGKPLDDHSLVDDICNRQDAVI
Sbjct: 121 KEFTFHVERDRDVAYVKEKIATKVKDFVDVVDEHEVVCDGKPLDDHSLVDDICNRQDAVI 180

Query: 181 HLFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNEDKEAYRTKYEYDK 240
           HLFVRKSAKVRG+P++KNFELSIVASN +EQCKSEFSR NNQK Y EDKEAYRTKYEYDK
Sbjct: 181 HLFVRKSAKVRGKPVDKNFELSIVASNFEEQCKSEFSRENNQKVYKEDKEAYRTKYEYDK 240

Query: 241 EIGPRRPSPNGGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYLM 300
           EI PRRP PNGGSV+EPIV+NPK+ELPIPIWDMVN+TVDGLD GHFPVRSLEGTGGAYLM
Sbjct: 241 EIAPRRPYPNGGSVLEPIVVNPKVELPIPIWDMVNSTVDGLDCGHFPVRSLEGTGGAYLM 300

Query: 301 LDPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPI 360
           LD SGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPI
Sbjct: 301 LDRSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPI 360

Query: 361 SGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF 420
           SGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF
Sbjct: 361 SGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF 420

Query: 421 PTKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLY 480
            TKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLY
Sbjct: 421 STKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLY 480

Query: 481 WPQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP 540
           WPQ+QQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP
Sbjct: 481 WPQSQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP 540

Query: 541 FDIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLDQIAASLS 598
           FDIGSFMCRETL KKSMIEECVEEAMDSL+PGTSEATFLESVYEIMD RLDQIAASLS
Sbjct: 541 FDIGSFMCRETLTKKSMIEECVEEAMDSLVPGTSEATFLESVYEIMDRRLDQIAASLS 598

BLAST of Bhi12M002069 vs. NCBI nr
Match: XP_004140586.1 (phosphatidylinositol 4-kinase gamma 4 [Cucumis sativus] >KGN46357.1 hypothetical protein Csa_005421 [Cucumis sativus])

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 561/598 (93.81%), Postives = 575/598 (96.15%), Query Frame = 0

Query: 1   MSSTGVTALCPVHNEHLFSHNRLITPSILESNESIWIYVSVSGSMSPMPIFASESIESVK 60
           MSS GV ALCPV NEHL  H RLITPS LESNESIWIYVSVSGSMSPMPIFASESIESVK
Sbjct: 1   MSSAGVIALCPVRNEHLVYHKRLITPSALESNESIWIYVSVSGSMSPMPIFASESIESVK 60

Query: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTHCG 120
           LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKT+CG
Sbjct: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTYCG 120

Query: 121 KEFTFHVERDRDIAYVKEKIATKVKEFVD-VDDHEVVCDGKPLDDHSLVDDICNRQDAVI 180
           KEFTFHVERDRD+AYVKEKIATKVKEFVD VD+HEVVCDG PLDDHSLVDDICNRQDAVI
Sbjct: 121 KEFTFHVERDRDVAYVKEKIATKVKEFVDVVDEHEVVCDGNPLDDHSLVDDICNRQDAVI 180

Query: 181 HLFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNEDKEAYRTKYEYDK 240
           HLFVRKSAKVRGRP++KNFELSIVASN +EQCKSEFSR NNQKEYNEDKEAYRTKYEYDK
Sbjct: 181 HLFVRKSAKVRGRPVDKNFELSIVASNFEEQCKSEFSRENNQKEYNEDKEAYRTKYEYDK 240

Query: 241 EIGPRRPSPNGGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYLM 300
           E  P RP PNGGSVMEPIV+NPK+ELP PIWDMVN+TVDGLD GHFPV SLEGTGGAYLM
Sbjct: 241 ESAP-RPYPNGGSVMEPIVVNPKVELPKPIWDMVNSTVDGLDCGHFPVSSLEGTGGAYLM 300

Query: 301 LDPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPI 360
           LD SGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGT+VGEGAFREVAAYLLDHPI
Sbjct: 301 LDRSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTRVGEGAFREVAAYLLDHPI 360

Query: 361 SGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF 420
           SGRRSMFGDKNGFAGVPPTALV+CLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF
Sbjct: 361 SGRRSMFGDKNGFAGVPPTALVQCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF 420

Query: 421 PTKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLY 480
           PTKEVHKISVLDIRLANADRHAGNILLGKEREG Q VLIPIDHGYCLPTSFEDCTFDWLY
Sbjct: 421 PTKEVHKISVLDIRLANADRHAGNILLGKEREGDQVVLIPIDHGYCLPTSFEDCTFDWLY 480

Query: 481 WPQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP 540
           WPQ+QQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP
Sbjct: 481 WPQSQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP 540

Query: 541 FDIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLDQIAASLS 598
           FDIGSFMCRETL KKSMIEECVEEA+DSL+PGTSEATFLESVYEIMD RLDQIAASLS
Sbjct: 541 FDIGSFMCRETLTKKSMIEECVEEAIDSLVPGTSEATFLESVYEIMDLRLDQIAASLS 597

BLAST of Bhi12M002069 vs. NCBI nr
Match: XP_022157795.1 (phosphatidylinositol 4-kinase gamma 4 [Momordica charantia])

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 530/599 (88.48%), Postives = 562/599 (93.82%), Query Frame = 0

Query: 1   MSSTGVTALCPVHNEHLFSHNRLITPSILESNESIWIYVSVSGSMSPMPIFASESIESVK 60
           MSS GVTAL PVHNE L   NRL TP  LE NESIWIY+SVSGS+ PMPIFASESIESVK
Sbjct: 1   MSSAGVTALLPVHNEQLLFPNRLPTPLGLEPNESIWIYLSVSGSIDPMPIFASESIESVK 60

Query: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTHCG 120
           LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKT+CG
Sbjct: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTYCG 120

Query: 121 KEFTFHVERDRDIAYVKEKIATKVKEFVDVDDHEVVCDGKPLDDHSLVDDICNRQDAVIH 180
           KEFTFHVERDRD+AYVKEKI  KVKEFVD D+HEVVCDG+PLDDHSLVDDICNR+DAVIH
Sbjct: 121 KEFTFHVERDRDVAYVKEKITKKVKEFVDADEHEVVCDGRPLDDHSLVDDICNRKDAVIH 180

Query: 181 LFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNE-DKEAYRTKYEYDK 240
           LFVRKSAKVRG+P++K FELSIVAS+ KEQC S+FSR N++K+YN  D++ YRT+Y+YDK
Sbjct: 181 LFVRKSAKVRGKPVDKAFELSIVASHFKEQCGSKFSRENDKKQYNNTDEKVYRTEYDYDK 240

Query: 241 EIGPRRPSPN--GGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAY 300
           EI  RRP PN   GS MEPI++NPKIELPIPIWDMVN+T+DGLD GHFPVRSLEGTGGAY
Sbjct: 241 EIASRRPPPNEGRGSFMEPIIVNPKIELPIPIWDMVNSTLDGLDHGHFPVRSLEGTGGAY 300

Query: 301 LMLDPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDH 360
           LMLDPSGKKYVSVFKPIDEEPMALNNPR LPLSLDGEGLKKGT+VGEGAFREVAAY+LDH
Sbjct: 301 LMLDPSGKKYVSVFKPIDEEPMALNNPRALPLSLDGEGLKKGTRVGEGAFREVAAYILDH 360

Query: 361 PISGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAG 420
           PI+G RSMFGDK GFAGVPPT LVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCE+FGAG
Sbjct: 361 PITGHRSMFGDKKGFAGVPPTVLVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEEFGAG 420

Query: 421 AFPTKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDW 480
           AFPTKEVHKISVLDIRLANADRHAGNILLGKEREGG TVLIPIDHGYCLPTSFEDCTFDW
Sbjct: 421 AFPTKEVHKISVLDIRLANADRHAGNILLGKEREGGHTVLIPIDHGYCLPTSFEDCTFDW 480

Query: 481 LYWPQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGL 540
           LYWPQAQQPYDAETLDYINSLDAEEDIALLKFHGW+LPLECARTLRISTMLLKKGAKRGL
Sbjct: 481 LYWPQAQQPYDAETLDYINSLDAEEDIALLKFHGWELPLECARTLRISTMLLKKGAKRGL 540

Query: 541 TPFDIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLDQIAASL 597
           TPFDIGS MCRETL KKS+IEECV+EAMDS+LPGTSE TFLE+VYEIMDHRLDQIAASL
Sbjct: 541 TPFDIGSAMCRETLTKKSVIEECVDEAMDSILPGTSETTFLETVYEIMDHRLDQIAASL 599

BLAST of Bhi12M002069 vs. ExPASy TrEMBL
Match: A0A1S3CBK0 (1-phosphatidylinositol 4-kinase OS=Cucumis melo OX=3656 GN=LOC103498938 PE=3 SV=1)

HSP 1 Score: 1156.0 bits (2989), Expect = 0.0e+00
Identity = 565/598 (94.48%), Postives = 580/598 (96.99%), Query Frame = 0

Query: 1   MSSTGVTALCPVHNEHLFSHNRLITPSILESNESIWIYVSVSGSMSPMPIFASESIESVK 60
           MSSTGV ALCP  NEHL SH RLITPS L+SNESIWIYVSVSGSMSPMPIFASESIESVK
Sbjct: 1   MSSTGVIALCPARNEHLVSHKRLITPSALQSNESIWIYVSVSGSMSPMPIFASESIESVK 60

Query: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTHCG 120
           LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKT+CG
Sbjct: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTYCG 120

Query: 121 KEFTFHVERDRDIAYVKEKIATKVKEFVD-VDDHEVVCDGKPLDDHSLVDDICNRQDAVI 180
           KEFTFHVERDRD+AYVKEKIATKVK+FVD VD+HEVVCDGKPLDDHSLVDDICNRQDAVI
Sbjct: 121 KEFTFHVERDRDVAYVKEKIATKVKDFVDVVDEHEVVCDGKPLDDHSLVDDICNRQDAVI 180

Query: 181 HLFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNEDKEAYRTKYEYDK 240
           HLFVRKSAKVRG+P++KNFELSIVASN +EQCKSEFSR NNQK Y EDKEAYRTKYEYDK
Sbjct: 181 HLFVRKSAKVRGKPVDKNFELSIVASNFEEQCKSEFSRENNQKVYKEDKEAYRTKYEYDK 240

Query: 241 EIGPRRPSPNGGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYLM 300
           EI PRRP PNGGSV+EPIV+NPK+ELPIPIWDMVN+TVDGLD GHFPVRSLEGTGGAYLM
Sbjct: 241 EIAPRRPYPNGGSVLEPIVVNPKVELPIPIWDMVNSTVDGLDCGHFPVRSLEGTGGAYLM 300

Query: 301 LDPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPI 360
           LD SGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPI
Sbjct: 301 LDRSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPI 360

Query: 361 SGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF 420
           SGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF
Sbjct: 361 SGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF 420

Query: 421 PTKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLY 480
            TKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLY
Sbjct: 421 STKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLY 480

Query: 481 WPQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP 540
           WPQ+QQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP
Sbjct: 481 WPQSQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP 540

Query: 541 FDIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLDQIAASLS 598
           FDIGSFMCRETL KKSMIEECVEEAMDSL+PGTSEATFLESVYEIMD RLDQIAASLS
Sbjct: 541 FDIGSFMCRETLTKKSMIEECVEEAMDSLVPGTSEATFLESVYEIMDRRLDQIAASLS 598

BLAST of Bhi12M002069 vs. ExPASy TrEMBL
Match: A0A5D3DLS4 (1-phosphatidylinositol 4-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001420 PE=3 SV=1)

HSP 1 Score: 1154.4 bits (2985), Expect = 0.0e+00
Identity = 564/598 (94.31%), Postives = 579/598 (96.82%), Query Frame = 0

Query: 1   MSSTGVTALCPVHNEHLFSHNRLITPSILESNESIWIYVSVSGSMSPMPIFASESIESVK 60
           MSSTGV ALCP  NEHL SH RL TPS L+SNESIWIYVSVSGSMSPMPIFASESIESVK
Sbjct: 1   MSSTGVIALCPARNEHLVSHKRLFTPSALQSNESIWIYVSVSGSMSPMPIFASESIESVK 60

Query: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTHCG 120
           LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKT+CG
Sbjct: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTYCG 120

Query: 121 KEFTFHVERDRDIAYVKEKIATKVKEFVD-VDDHEVVCDGKPLDDHSLVDDICNRQDAVI 180
           KEFTFHVERDRD+AYVKEKIATKVK+FVD VD+HEVVCDGKPLDDHSLVDDICNRQDAVI
Sbjct: 121 KEFTFHVERDRDVAYVKEKIATKVKDFVDVVDEHEVVCDGKPLDDHSLVDDICNRQDAVI 180

Query: 181 HLFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNEDKEAYRTKYEYDK 240
           HLFVRKSAKVRG+P++KNFELSIVASN +EQCKSEFSR NNQK Y EDKEAYRTKYEYDK
Sbjct: 181 HLFVRKSAKVRGKPVDKNFELSIVASNFEEQCKSEFSRENNQKVYKEDKEAYRTKYEYDK 240

Query: 241 EIGPRRPSPNGGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYLM 300
           EI PRRP PNGGSV+EPIV+NPK+ELPIPIWDMVN+TVDGLD GHFPVRSLEGTGGAYLM
Sbjct: 241 EIAPRRPYPNGGSVLEPIVVNPKVELPIPIWDMVNSTVDGLDCGHFPVRSLEGTGGAYLM 300

Query: 301 LDPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPI 360
           LD SGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPI
Sbjct: 301 LDRSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPI 360

Query: 361 SGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF 420
           SGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF
Sbjct: 361 SGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF 420

Query: 421 PTKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLY 480
            TKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLY
Sbjct: 421 STKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLY 480

Query: 481 WPQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP 540
           WPQ+QQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP
Sbjct: 481 WPQSQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP 540

Query: 541 FDIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLDQIAASLS 598
           FDIGSFMCRETL KKSMIEECVEEAMDSL+PGTSEATFLESVYEIMD RLDQIAASLS
Sbjct: 541 FDIGSFMCRETLTKKSMIEECVEEAMDSLVPGTSEATFLESVYEIMDRRLDQIAASLS 598

BLAST of Bhi12M002069 vs. ExPASy TrEMBL
Match: A0A0A0KD97 (1-phosphatidylinositol 4-kinase OS=Cucumis sativus OX=3659 GN=Csa_6G087850 PE=3 SV=1)

HSP 1 Score: 1140.2 bits (2948), Expect = 0.0e+00
Identity = 561/598 (93.81%), Postives = 575/598 (96.15%), Query Frame = 0

Query: 1   MSSTGVTALCPVHNEHLFSHNRLITPSILESNESIWIYVSVSGSMSPMPIFASESIESVK 60
           MSS GV ALCPV NEHL  H RLITPS LESNESIWIYVSVSGSMSPMPIFASESIESVK
Sbjct: 1   MSSAGVIALCPVRNEHLVYHKRLITPSALESNESIWIYVSVSGSMSPMPIFASESIESVK 60

Query: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTHCG 120
           LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKT+CG
Sbjct: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTYCG 120

Query: 121 KEFTFHVERDRDIAYVKEKIATKVKEFVD-VDDHEVVCDGKPLDDHSLVDDICNRQDAVI 180
           KEFTFHVERDRD+AYVKEKIATKVKEFVD VD+HEVVCDG PLDDHSLVDDICNRQDAVI
Sbjct: 121 KEFTFHVERDRDVAYVKEKIATKVKEFVDVVDEHEVVCDGNPLDDHSLVDDICNRQDAVI 180

Query: 181 HLFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNEDKEAYRTKYEYDK 240
           HLFVRKSAKVRGRP++KNFELSIVASN +EQCKSEFSR NNQKEYNEDKEAYRTKYEYDK
Sbjct: 181 HLFVRKSAKVRGRPVDKNFELSIVASNFEEQCKSEFSRENNQKEYNEDKEAYRTKYEYDK 240

Query: 241 EIGPRRPSPNGGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYLM 300
           E  P RP PNGGSVMEPIV+NPK+ELP PIWDMVN+TVDGLD GHFPV SLEGTGGAYLM
Sbjct: 241 ESAP-RPYPNGGSVMEPIVVNPKVELPKPIWDMVNSTVDGLDCGHFPVSSLEGTGGAYLM 300

Query: 301 LDPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPI 360
           LD SGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGT+VGEGAFREVAAYLLDHPI
Sbjct: 301 LDRSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTRVGEGAFREVAAYLLDHPI 360

Query: 361 SGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF 420
           SGRRSMFGDKNGFAGVPPTALV+CLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF
Sbjct: 361 SGRRSMFGDKNGFAGVPPTALVQCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAGAF 420

Query: 421 PTKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDWLY 480
           PTKEVHKISVLDIRLANADRHAGNILLGKEREG Q VLIPIDHGYCLPTSFEDCTFDWLY
Sbjct: 421 PTKEVHKISVLDIRLANADRHAGNILLGKEREGDQVVLIPIDHGYCLPTSFEDCTFDWLY 480

Query: 481 WPQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP 540
           WPQ+QQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP
Sbjct: 481 WPQSQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGLTP 540

Query: 541 FDIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLDQIAASLS 598
           FDIGSFMCRETL KKSMIEECVEEA+DSL+PGTSEATFLESVYEIMD RLDQIAASLS
Sbjct: 541 FDIGSFMCRETLTKKSMIEECVEEAIDSLVPGTSEATFLESVYEIMDLRLDQIAASLS 597

BLAST of Bhi12M002069 vs. ExPASy TrEMBL
Match: A0A6J1DU29 (1-phosphatidylinositol 4-kinase OS=Momordica charantia OX=3673 GN=LOC111024414 PE=3 SV=1)

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 530/599 (88.48%), Postives = 562/599 (93.82%), Query Frame = 0

Query: 1   MSSTGVTALCPVHNEHLFSHNRLITPSILESNESIWIYVSVSGSMSPMPIFASESIESVK 60
           MSS GVTAL PVHNE L   NRL TP  LE NESIWIY+SVSGS+ PMPIFASESIESVK
Sbjct: 1   MSSAGVTALLPVHNEQLLFPNRLPTPLGLEPNESIWIYLSVSGSIDPMPIFASESIESVK 60

Query: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTHCG 120
           LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKT+CG
Sbjct: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTYCG 120

Query: 121 KEFTFHVERDRDIAYVKEKIATKVKEFVDVDDHEVVCDGKPLDDHSLVDDICNRQDAVIH 180
           KEFTFHVERDRD+AYVKEKI  KVKEFVD D+HEVVCDG+PLDDHSLVDDICNR+DAVIH
Sbjct: 121 KEFTFHVERDRDVAYVKEKITKKVKEFVDADEHEVVCDGRPLDDHSLVDDICNRKDAVIH 180

Query: 181 LFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQKEYNE-DKEAYRTKYEYDK 240
           LFVRKSAKVRG+P++K FELSIVAS+ KEQC S+FSR N++K+YN  D++ YRT+Y+YDK
Sbjct: 181 LFVRKSAKVRGKPVDKAFELSIVASHFKEQCGSKFSRENDKKQYNNTDEKVYRTEYDYDK 240

Query: 241 EIGPRRPSPN--GGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAY 300
           EI  RRP PN   GS MEPI++NPKIELPIPIWDMVN+T+DGLD GHFPVRSLEGTGGAY
Sbjct: 241 EIASRRPPPNEGRGSFMEPIIVNPKIELPIPIWDMVNSTLDGLDHGHFPVRSLEGTGGAY 300

Query: 301 LMLDPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDH 360
           LMLDPSGKKYVSVFKPIDEEPMALNNPR LPLSLDGEGLKKGT+VGEGAFREVAAY+LDH
Sbjct: 301 LMLDPSGKKYVSVFKPIDEEPMALNNPRALPLSLDGEGLKKGTRVGEGAFREVAAYILDH 360

Query: 361 PISGRRSMFGDKNGFAGVPPTALVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEDFGAG 420
           PI+G RSMFGDK GFAGVPPT LVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCE+FGAG
Sbjct: 361 PITGHRSMFGDKKGFAGVPPTVLVKCLHDGFNHPSDKSVKIGSLQMFMENNGSCEEFGAG 420

Query: 421 AFPTKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDW 480
           AFPTKEVHKISVLDIRLANADRHAGNILLGKEREGG TVLIPIDHGYCLPTSFEDCTFDW
Sbjct: 421 AFPTKEVHKISVLDIRLANADRHAGNILLGKEREGGHTVLIPIDHGYCLPTSFEDCTFDW 480

Query: 481 LYWPQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGL 540
           LYWPQAQQPYDAETLDYINSLDAEEDIALLKFHGW+LPLECARTLRISTMLLKKGAKRGL
Sbjct: 481 LYWPQAQQPYDAETLDYINSLDAEEDIALLKFHGWELPLECARTLRISTMLLKKGAKRGL 540

Query: 541 TPFDIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLDQIAASL 597
           TPFDIGS MCRETL KKS+IEECV+EAMDS+LPGTSE TFLE+VYEIMDHRLDQIAASL
Sbjct: 541 TPFDIGSAMCRETLTKKSVIEECVDEAMDSILPGTSETTFLETVYEIMDHRLDQIAASL 599

BLAST of Bhi12M002069 vs. ExPASy TrEMBL
Match: A0A6J1H4X2 (1-phosphatidylinositol 4-kinase OS=Cucurbita moschata OX=3662 GN=LOC111460605 PE=3 SV=1)

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 526/600 (87.67%), Postives = 562/600 (93.67%), Query Frame = 0

Query: 1   MSSTGVTALCPVHNEHLFSHNRLITPSILESNESIWIYVSVSGSMSPMPIFASESIESVK 60
           MSS GV ALCPV N+HLFS NRLI PS+LES+ESIWIYVSVSGSMSPMPIFASESIESVK
Sbjct: 1   MSSMGVIALCPVQNDHLFSPNRLIAPSVLESDESIWIYVSVSGSMSPMPIFASESIESVK 60

Query: 61  LRIQSCKGFVVKKQKLVCGGRELSRNNSLVRDYGVTDGNVLHLVLRLSDLQVINVKTHCG 120
           LRIQSCKGFVVKKQKLVCGGRELSRN SLVRDYGVTDGNVLHLVLRLSDLQVINVKT+CG
Sbjct: 61  LRIQSCKGFVVKKQKLVCGGRELSRNRSLVRDYGVTDGNVLHLVLRLSDLQVINVKTYCG 120

Query: 121 KEFTFHVERDRDIAYVKEKIATKVKEFVDVDDHEVVCDGKPLDDHSLVDDICNRQDAVIH 180
           KEFTFHVERDRDI+YVKEKI  KVKEFVD D+HEVVC G+ LDDHSLV DICNR+DAVIH
Sbjct: 121 KEFTFHVERDRDISYVKEKIGKKVKEFVDTDEHEVVCGGRALDDHSLVADICNRKDAVIH 180

Query: 181 LFVRKSAKVRGRPIEKNFELSIVASNLKEQCKSEFSRTNNQ-KEYNEDKEAYRTKYEYDK 240
           LFVRKSAKVRGRP++KNFELSIVASN KEQCKSEFSR ++Q K+YNED + Y T YEYDK
Sbjct: 181 LFVRKSAKVRGRPVDKNFELSIVASNFKEQCKSEFSRGSDQKKKYNEDTKVYTTNYEYDK 240

Query: 241 EIGPRRPSPNGGSVMEPIVINPKIELPIPIWDMVNATVDGLDRGHFPVRSLEGTGGAYLM 300
           +I PRRPSPNGGS MEPIV+NPKIE+PIPIWDM+N+T+DGLD GHFPVRSLEGTGGAYLM
Sbjct: 241 KIAPRRPSPNGGSFMEPIVVNPKIEIPIPIWDMLNSTIDGLDHGHFPVRSLEGTGGAYLM 300

Query: 301 LDPSGKKYVSVFKPIDEEPMALNNPRGLPLSLDGEGLKKGTKVGEGAFREVAAYLLDHPI 360
           LD SGKKYVSVFKPIDEEPMA+NNPRGLPLSLDGEGLKKGT+VGEGAFREVAAY+LDHP+
Sbjct: 301 LDSSGKKYVSVFKPIDEEPMAVNNPRGLPLSLDGEGLKKGTRVGEGAFREVAAYILDHPV 360

Query: 361 SGRRSMFGDKNGFAGVPPTALVKCLHDGF--NHPSDKSVKIGSLQMFMENNGSCEDFGAG 420
           SG RSMFG+ NGFAGVPPT  VKC HDGF  +H S+KS+KIGSLQMFMENNGSCEDFGAG
Sbjct: 361 SGHRSMFGNDNGFAGVPPTVFVKCFHDGFSQSHRSEKSIKIGSLQMFMENNGSCEDFGAG 420

Query: 421 AFPTKEVHKISVLDIRLANADRHAGNILLGKEREGGQTVLIPIDHGYCLPTSFEDCTFDW 480
           AFPTKEVHKISVLDIRLANADRHAGNILLGKEREGG+TVLIPIDHGYCLPTSFEDCTF+W
Sbjct: 421 AFPTKEVHKISVLDIRLANADRHAGNILLGKEREGGRTVLIPIDHGYCLPTSFEDCTFEW 480

Query: 481 LYWPQAQQPYDAETLDYINSLDAEEDIALLKFHGWDLPLECARTLRISTMLLKKGAKRGL 540
           LYWPQAQQPYDAETLDYI SLDAEEDI LLKFHGW+LPLECARTLRISTMLLKKGAKRGL
Sbjct: 481 LYWPQAQQPYDAETLDYIQSLDAEEDIGLLKFHGWELPLECARTLRISTMLLKKGAKRGL 540

Query: 541 TPFDIGSFMCRETLMKKSMIEECVEEAMDSLLPGTSEATFLESVYEIMDHRLDQIAASLS 598
           TPF+IG+ MCRE L KKS+IEECVEEAMDSLLPGTSEA FLE+VYEI+DHRLD IAASLS
Sbjct: 541 TPFEIGNIMCRENLTKKSVIEECVEEAMDSLLPGTSEAAFLETVYEIIDHRLDLIAASLS 600

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT2G46500.16.3e-20761.15phosphoinositide 4-kinase gamma 4 [more]
AT2G46500.26.3e-20761.15phosphoinositide 4-kinase gamma 4 [more]
AT5G24240.13.4e-18455.73Phosphatidylinositol 3- and 4-kinase ;Ubiquitin family protein [more]
AT2G46500.32.0e-12856.74phosphoinositide 4-kinase gamma 4 [more]
AT1G64460.11.1e-11867.33Protein kinase superfamily protein [more]
Match NameE-valueIdentityDescription
Q9ZPY98.8e-20661.15Phosphatidylinositol 4-kinase gamma 4 OS=Arabidopsis thaliana OX=3702 GN=PI4KG4 ... [more]
Q9FNF84.7e-18355.73Phosphatidylinositol 4-kinase gamma 3 OS=Arabidopsis thaliana OX=3702 GN=PI4KG3 ... [more]
Q9SGW81.7e-17754.27Phosphatidylinositol 4-kinase gamma 2 OS=Arabidopsis thaliana OX=3702 GN=PI4KG2 ... [more]
Q6K8816.0e-17051.67Phosphatidylinositol 4-kinase gamma 4 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q8W4R84.9e-5537.50Phosphatidylinositol 4-kinase gamma 6 OS=Arabidopsis thaliana OX=3702 GN=PI4KG6 ... [more]
Match NameE-valueIdentityDescription
XP_038874462.10.0e+00100.00phosphatidylinositol 4-kinase gamma 4 [Benincasa hispida][more]
XP_008459989.10.0e+0094.48PREDICTED: phosphatidylinositol 4-kinase gamma 4 [Cucumis melo][more]
KAA0039904.10.0e+0094.31phosphatidylinositol 4-kinase gamma 4 [Cucumis melo var. makuwa] >TYK24596.1 pho... [more]
XP_004140586.10.0e+0093.81phosphatidylinositol 4-kinase gamma 4 [Cucumis sativus] >KGN46357.1 hypothetical... [more]
XP_022157795.10.0e+0088.48phosphatidylinositol 4-kinase gamma 4 [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A1S3CBK00.0e+0094.481-phosphatidylinositol 4-kinase OS=Cucumis melo OX=3656 GN=LOC103498938 PE=3 SV=... [more]
A0A5D3DLS40.0e+0094.311-phosphatidylinositol 4-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A0A0KD970.0e+0093.811-phosphatidylinositol 4-kinase OS=Cucumis sativus OX=3659 GN=Csa_6G087850 PE=3 ... [more]
A0A6J1DU290.0e+0088.481-phosphatidylinositol 4-kinase OS=Momordica charantia OX=3673 GN=LOC111024414 P... [more]
A0A6J1H4X20.0e+0087.671-phosphatidylinositol 4-kinase OS=Cucurbita moschata OX=3662 GN=LOC111460605 PE... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000626Ubiquitin-like domainSMARTSM00213ubq_7coord: 35..106
e-value: 1.7E-5
score: 34.3
coord: 111..184
e-value: 3.1E-4
score: 30.1
IPR000626Ubiquitin-like domainPFAMPF00240ubiquitincoord: 38..107
e-value: 6.7E-12
score: 45.0
coord: 113..185
e-value: 7.5E-9
score: 35.2
IPR000626Ubiquitin-like domainPROSITEPS50053UBIQUITIN_2coord: 35..106
score: 11.812365
IPR000626Ubiquitin-like domainPROSITEPS50053UBIQUITIN_2coord: 113..188
score: 11.507508
IPR000403Phosphatidylinositol 3-/4-kinase, catalytic domainPFAMPF00454PI3_PI4_kinasecoord: 292..541
e-value: 1.8E-50
score: 172.1
NoneNo IPR availableGENE3D3.10.20.90coord: 13..109
e-value: 9.8E-12
score: 46.7
NoneNo IPR availableGENE3D3.10.20.90coord: 111..197
e-value: 5.3E-11
score: 44.0
NoneNo IPR availablePANTHERPTHR45800:SF24PHOSPHATIDYLINOSITOL 4-KINASE GAMMA 4coord: 1..593
NoneNo IPR availableCDDcd17039Ubl_ubiquitin_likecoord: 113..182
e-value: 2.45846E-9
score: 51.8282
IPR044571Phosphatidylinositol 4-kinase gamma 1-8PANTHERPTHR45800PHOSPHATIDYLINOSITOL 4-KINASE GAMMAcoord: 1..593
IPR029071Ubiquitin-like domain superfamilySUPERFAMILY54236Ubiquitin-likecoord: 32..108
IPR029071Ubiquitin-like domain superfamilySUPERFAMILY54236Ubiquitin-likecoord: 108..185
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 403..593

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Bhi12G002069Bhi12G002069gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi12M002069.exon2Bhi12M002069.exon2exon
Bhi12M002069.exon1Bhi12M002069.exon1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi12M002069.utr3p1Bhi12M002069.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Bhi12M002069cds.Bhi12M002069_2CDS
cds.Bhi12M002069cds.Bhi12M002069CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi12M002069.utr5p1Bhi12M002069.utr5p1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Bhi12M002069Bhi12M002069-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016310 phosphorylation
biological_process GO:0046854 phosphatidylinositol phosphate biosynthetic process
molecular_function GO:0005515 protein binding
molecular_function GO:0004430 1-phosphatidylinositol 4-kinase activity