Homology
BLAST of Bhi12M000319 vs. TAIR 10
Match:
AT1G04110.1 (Subtilase family protein )
HSP 1 Score: 399.1 bits (1024), Expect = 5.5e-111
Identity = 231/511 (45.21%), Postives = 313/511 (61.25%), Query Frame = 0
Query: 32 TYIVHV-KKPETADDLES---WHRSFLPTS---LENSEEQPS--LLYSYRNVMSGFAARL 91
TYIV + ETA S WH SFL + +E EE+PS LLYSY + + GFAA+L
Sbjct: 27 TYIVQLHPNSETAKTFASKFDWHLSFLQEAVLGVEEEEEEPSSRLLYSYGSAIEGFAAQL 86
Query: 92 TEEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLN--RQFGFWKDSNFGKGVIIGVLD 151
TE + + + V+ R + +L + TT++ FLGL+ G W S FG+G IIGVLD
Sbjct: 87 TESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFGNSGVWSKSRFGQGTIIGVLD 146
Query: 152 GGITPNHPSFDDVGMPQPPTKWKGRCE----FNFSACNNKLIGARSFNLASKALKGH--A 211
G+ P PSFDD GMP P KWKG C+ F+ S+CN KLIGAR F ++GH A
Sbjct: 147 TGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRKLIGARFF------IRGHRVA 206
Query: 212 TTMDDSP---------IDEDGHGTHTASTAAGTFIDGAEALGNAI---------AHLAIY 271
+ ++SP D GHGTHTAST G+ + A LGN AH+A+Y
Sbjct: 207 NSPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAGVARGMAPGAHIAVY 266
Query: 272 KVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKGIFVRC 331
KVC+ C +DILAA+D A++D VDVLSLSLG P ++D+IAIG F A+++GI V C
Sbjct: 267 KVCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAIGTFRAMERGISVIC 326
Query: 332 SAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEELDGESLFQ----PNDFPQ 391
+A N+GP ++++N APW+ T+ A T+DRR A +L NG+ L GESL+ N +
Sbjct: 327 AAGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGESLYPGKGIKNAGRE 386
Query: 392 TFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMIL 451
+ V G+K C GSL +++GK+V+C+RG R KG VK AGG AMIL
Sbjct: 387 VEVIYVTGGDKGS--EFCLRGSLPREEIRGKMVICDRGVN-GRSEKGEAVKEAGGVAMIL 446
Query: 452 LNTESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPKATIVFKGTIIGDDFSPAIA 504
NTE + D H+LPA+ + +T ++ +KAY+N+T PKA I+F GT+IG +P +A
Sbjct: 447 ANTEINQEEDSIDVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGTVIGRSRAPEVA 506
BLAST of Bhi12M000319 vs. TAIR 10
Match:
AT3G14067.1 (Subtilase family protein )
HSP 1 Score: 392.1 bits (1006), Expect = 6.8e-109
Identity = 246/538 (45.72%), Postives = 319/538 (59.29%), Query Frame = 0
Query: 4 LPSLFLL--LLLKFHGYEAQLTELPLSSLHTYIVHVK---KPETADDLESWHRSFLPTSL 63
L S+F + LLL F + ++ L +YIVHV+ KP +WH S L SL
Sbjct: 6 LSSIFFVFPLLLCFFSPSSSSSD----GLESYIVHVQRSHKPSLFSSHNNWHVSLL-RSL 65
Query: 64 ENSEEQPSLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGL 123
+S + +LLYSY + GF+ARL+ Q A+ +S ++ +HTTHTP FLG
Sbjct: 66 PSSPQPATLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGF 125
Query: 124 NRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPTKWKGRCE----FNFSACN 183
++ G W +SN+G+ VI+GVLD GI P HPSF D G+ P+ WKG CE F S+CN
Sbjct: 126 SQNSGLWSNSNYGEDVIVGVLDTGIWPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCN 185
Query: 184 NKLIGARSFN----LASKALKGHATTMDDSPIDEDGHGTHTASTAAGTFIDGAE----AL 243
KLIGAR+F K HA SP D +GHGTHTASTAAG+ + A A
Sbjct: 186 RKLIGARAFYRGYLTQRNGTKKHAAKESRSPRDTEGHGTHTASTAAGSVVANASLYQYAR 245
Query: 244 GNAI-----AHLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLG-SPSAP-FFEDS 303
G A A +A YK+C+ C D+DILAA+D AV DGV V+SLS+G S SAP + DS
Sbjct: 246 GTATGMASKARIAAYKICWTGGCYDSDILAAMDQAVADGVHVISLSVGASGSAPEYHTDS 305
Query: 304 IAIGAFTAIQKGIFVRCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEEL 363
IAIGAF A + GI V CSA NSGP T +N APWILTV AST+DR A A G+G+
Sbjct: 306 IAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGASTVDREFAANAITGDGKVF 365
Query: 364 DGESLFQPNDFPQTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKG 423
G SL+ P + L LV+ G+ LC G L + V+GK+V+C+RGG AR+ KG
Sbjct: 366 TGTSLYAGESLPDSQLSLVYSGDCGS--RLCYPGKLNSSLVEGKIVLCDRGGN-ARVEKG 425
Query: 424 VEVKNAGGAAMILLNTESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPKATIVFK 483
VK AGGA MIL NT G AD+H++PA+ V A +I+ YI ++ P A I F
Sbjct: 426 SAVKLAGGAGMILANTAESGEELTADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFL 485
Query: 484 GTIIG-DDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWP---FPLDNNTNPK 514
GT+IG SP +A+FSSRGP+ +P ILKPD+ PGV+ILA W P D + +P+
Sbjct: 486 GTLIGPSPPSPRVAAFSSRGPNHLTPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPR 535
BLAST of Bhi12M000319 vs. TAIR 10
Match:
AT2G05920.1 (Subtilase family protein )
HSP 1 Score: 385.6 bits (989), Expect = 6.3e-107
Identity = 224/491 (45.62%), Postives = 297/491 (60.49%), Query Frame = 0
Query: 32 TYIV---HVKKPETADDLESWHRSFLPTSLENSEEQPSLLYSYRNVMSGFAARL-TEEQV 91
TYI+ H KPE+ W+ TS NSE SLLY+Y GF+A L + E
Sbjct: 29 TYIIRVNHSDKPESFLTHHDWY-----TSQLNSES--SLLYTYTTSFHGFSAYLDSTEAD 88
Query: 92 KAMEEKDGFVSARREKILHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNH 151
+ + + + + LHTT TP+FLGLN +FG + GVIIGVLD G+ P
Sbjct: 89 SLLSSSNSILDIFEDPLYTLHTTRTPEFLGLNSEFGVHDLGSSSNGVIIGVLDTGVWPES 148
Query: 152 PSFDDVGMPQPPTKWKGRCE----FNFSACNNKLIGARSFNLASKALKG---HATTMDDS 211
SFDD MP+ P+KWKG CE F+ CN KLIGARSF+ + G + S
Sbjct: 149 RSFDDTDMPEIPSKWKGECESGSDFDSKLCNKKLIGARSFSKGFQMASGGGFSSKRESVS 208
Query: 212 PIDEDGHGTHTASTAAGTFIDGAEALGNAI---------AHLAIYKVCFGEDCPDTDILA 271
P D DGHGTHT++TAAG+ + A LG A A +A YKVC+ C +DILA
Sbjct: 209 PRDVDGHGTHTSTTAAGSAVRNASFLGYAAGTARGMATRARVATYKVCWSTGCFGSDILA 268
Query: 272 ALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKGIFVRCSAANSGPFNATLSNE 331
A+D A+ DGVDVLSLSLG SAP++ D+IAIGAF+A+++G+FV CSA NSGP A+++N
Sbjct: 269 AMDRAILDGVDVLSLSLGGGSAPYYRDTIAIGAFSAMERGVFVSCSAGNSGPTRASVANV 328
Query: 332 APWILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDFPQTFLPLVFPGEKNETVALCG 391
APW++TV A T+DR A A LGNG+ L G SL+ L LV+ + + LC
Sbjct: 329 APWVMTVGAGTLDRDFPAFANLGNGKRLTGVSLYSGVGMGTKPLELVYNKGNSSSSNLCL 388
Query: 392 EGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNTESDGFTTEADAHVLPA 451
GSL + V+GK+VVC+RG AR+ KG V++AGG MI+ NT + G AD+H+LPA
Sbjct: 389 PGSLDSSIVRGKIVVCDRGVN-ARVEKGAVVRDAGGLGMIMANTAASGEELVADSHLLPA 448
Query: 452 SHVSHTAALKIKAYINSTTYPKATIVFKGTIIGDDFSPAIASFSSRGPSLASPGILKPDI 503
V ++ Y+ S + P A +VFKGT++ SP +A+FSSRGP+ +P ILKPD+
Sbjct: 449 IAVGKKTGDLLREYVKSDSKPTALLVFKGTVLDVKPSPVVAAFSSRGPNTVTPEILKPDV 508
BLAST of Bhi12M000319 vs. TAIR 10
Match:
AT3G14240.1 (Subtilase family protein )
HSP 1 Score: 377.9 bits (969), Expect = 1.3e-104
Identity = 231/503 (45.92%), Postives = 303/503 (60.24%), Query Frame = 0
Query: 32 TYIVHV---KKPETADDLESWHRSFLPTSLENSEEQPSLLYSYRNVMSGFAARLTEEQVK 91
TYIVHV KP W+ S L SL +S PS++++Y V GF+ARLT +
Sbjct: 27 TYIVHVDHEAKPSIFPTHFHWYTSSL-ASLTSS--PPSIIHTYDTVFHGFSARLTSQDAS 86
Query: 92 AMEEKDGFVSARREKILHLHTTHTPDFLGLNR--QFGFWKDSNFGKGVIIGVLDGGITPN 151
+ + +S E++ HLHTT +P+FLGL + G ++S+FG ++IGV+D G+ P
Sbjct: 87 QLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGVWPE 146
Query: 152 HPSFDDVGMPQPPTKWKGRC----EFNFSACNNKLIGARSFNLASKALKG--HATTMDDS 211
PSFDD G+ P KWKG+C +F SACN KL+GAR F +A G + TT S
Sbjct: 147 RPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETTEFRS 206
Query: 212 PIDEDGHGTHTASTAAGTFIDGAEALGNA---------IAHLAIYKVCFGEDCPDTDILA 271
P D DGHGTHTAS +AG ++ A LG A A LA YKVC+ C D+DILA
Sbjct: 207 PRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSDILA 266
Query: 272 ALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKGIFVRCSAANSGPFNATLSNE 331
A D AV DGVDV+SLS+G P++ D+IAIGAF AI +GIFV SA N GP T++N
Sbjct: 267 AFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTVTNV 326
Query: 332 APWILTVAASTIDRRIVAAAKLGNGEELDGESLF-QPNDFPQTFLPLVFPGE----KNET 391
APW+ TV A TIDR A KLGNG+ + G S++ P P PLV+ G +
Sbjct: 327 APWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGDGYS 386
Query: 392 VALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNTESDGFTTEADA 451
+LC EGSL VKGK+V+C+RG +R KG V+ GG MI+ N DG AD
Sbjct: 387 SSLCLEGSLDPNLVKGKIVLCDRGIN-SRATKGEIVRKNGGLGMIIANGVFDGEGLVADC 446
Query: 452 HVLPASHVSHTAALKIKAYIN------STTYPKATIVFKGTIIGDDFSPAIASFSSRGPS 504
HVLPA+ V + +I+ YI+ S+ +P ATIVFKGT +G +P +ASFS+RGP+
Sbjct: 447 HVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGIRPAPVVASFSARGPN 506
BLAST of Bhi12M000319 vs. TAIR 10
Match:
AT5G67360.1 (Subtilase family protein )
HSP 1 Score: 374.8 bits (961), Expect = 1.1e-103
Identity = 220/515 (42.72%), Postives = 302/515 (58.64%), Query Frame = 0
Query: 6 SLFLLLLLKFHGYEAQLTELPLSSLHTYIVHVKKPETADDLESWHRSFLPTSLENSEEQP 65
+ FLLL L F + S TYIVH+ K + + H ++ +SL + +
Sbjct: 10 AFFLLLCLGFCHVSSS-----SSDQGTYIVHMAKSQMPSSFD-LHSNWYDSSLRSISDSA 69
Query: 66 SLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNRQFG-F 125
LLY+Y N + GF+ RLT+E+ ++ + G +S E LHTT TP FLGL+
Sbjct: 70 ELLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADL 129
Query: 126 WKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPTKWKGRCE----FNFSACNNKLIGA 185
+ ++ V++GVLD G+ P S+ D G P+ WKG CE F S CN KLIGA
Sbjct: 130 FPEAGSYSDVVVGVLDTGVWPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGA 189
Query: 186 RSFNLASKALKGHATTMDD--SPIDEDGHGTHTASTAAGTFIDGAEALGNAI-------- 245
R F ++ G + SP D+DGHGTHT+STAAG+ ++GA LG A
Sbjct: 190 RFFARGYESTMGPIDESKESRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAP 249
Query: 246 -AHLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQ 305
A +A+YKVC+ C +DILAA+D A+ D V+VLS+SLG + ++ D +AIGAF A++
Sbjct: 250 RARVAVYKVCWLGGCFSSDILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAME 309
Query: 306 KGIFVRCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEELDGESLFQPND 365
+GI V CSA N+GP +++LSN APWI TV A T+DR A A LGNG+ G SLF+
Sbjct: 310 RGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEA 369
Query: 366 FPQTFLPLVFPGEKNETV--ALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGG 425
P LP ++ G + LC G+L VKGK+V+C+RG AR+ KG VK AGG
Sbjct: 370 LPDKLLPFIYAGNASNATNGNLCMTGTLIPEKVKGKIVMCDRGIN-ARVQKGDVVKAAGG 429
Query: 426 AAMILLNTESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPKATIVFKGTIIGDDF 485
MIL NT ++G ADAH+LPA+ V A I+ Y+ + P A+I GT++G
Sbjct: 430 VGMILANTAANGEELVADAHLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVGVKP 489
Query: 486 SPAIASFSSRGPSLASPGILKPDITGPGVSILAAW 503
SP +A+FSSRGP+ +P ILKPD+ PGV+ILAAW
Sbjct: 490 SPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAW 517
BLAST of Bhi12M000319 vs. ExPASy Swiss-Prot
Match:
A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)
HSP 1 Score: 667.5 bits (1721), Expect = 1.2e-190
Identity = 340/529 (64.27%), Postives = 409/529 (77.32%), Query Frame = 0
Query: 9 LLLLLKFHGYEAQLTELP--------LSSLHTYIVHVKKPE-----TADDLESWHRSFLP 68
L LL FH + AQ +ELP SS YI+HV PE ++DLESW+ SFLP
Sbjct: 9 LTFLLTFHVHNAQGSELPTTTTESTETSSSKIYIIHVTGPEGKMLTESEDLESWYHSFLP 68
Query: 69 TSLENSEEQPSLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARREKILHLHTTHTPDF 128
+L +SEEQP ++YSY+NV+ GFAA LT+E++ A+E+K+GF+SA +++LH TTHTP F
Sbjct: 69 PTLMSSEEQPRVIYSYKNVLRGFAASLTQEELSAVEKKNGFISAHPQRVLHRQTTHTPKF 128
Query: 129 LGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPTKWKGRCEFNFSACNN 188
LGL + G WK+SNFGKGVIIGVLD GITP HPSF DVG+P PP KWKGRC+ N +ACNN
Sbjct: 129 LGLQQDTGVWKESNFGKGVIIGVLDSGITPGHPSFSDVGIPPPPPKWKGRCDLNVTACNN 188
Query: 189 KLIGARSFNLASKALKGHATTMDDSPIDEDGHGTHTASTAAGTFIDGAEALGNA------ 248
KLIGAR+FNLA++A+ G ++PIDEDGHGTHTASTAAG F++ AE LGNA
Sbjct: 189 KLIGARAFNLAAEAMNGKKA---EAPIDEDGHGTHTASTAAGAFVNYAEVLGNAKGTAAG 248
Query: 249 ---IAHLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLG-SPSAPFFEDSIAIGAF 308
AHLAIYKVCFGEDCP++DILAALDAAVEDGVDV+S+SLG S PFF DS AIGAF
Sbjct: 249 MAPHAHLAIYKVCFGEDCPESDILAALDAAVEDGVDVISISLGLSEPPPFFNDSTAIGAF 308
Query: 309 TAIQKGIFVRCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEELDGESLF 368
A+QKGIFV C+A NSGPFN+++ N APWILTV ASTIDRRIVA AKLGNG+E DGES+F
Sbjct: 309 AAMQKGIFVSCAAGNSGPFNSSIVNAAPWILTVGASTIDRRIVATAKLGNGQEFDGESVF 368
Query: 369 QPNDFPQTFLPLVFPGEK-NETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN 428
QP+ F T LPL + G+ E A C GSL + +GKVV+CERGGGIARIAKG EVK
Sbjct: 369 QPSSFTPTLLPLAYAGKNGKEESAFCANGSLDDSAFRGKVVLCERGGGIARIAKGEEVKR 428
Query: 429 AGGAAMILLNTESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPKATIVFKGTIIG 488
AGGAAMIL+N E++ F+ AD H LPA+HVS+ A ++IKAYINST P ATI+FKGT+IG
Sbjct: 429 AGGAAMILMNDETNAFSLSADVHALPATHVSYAAGIEIKAYINSTATPTATILFKGTVIG 488
Query: 489 DDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNPK 514
+ +PA+ASFSSRGP+L SPGILKPDI GPGV+ILAAWPFPL N+T+ K
Sbjct: 489 NSLAPAVASFSSRGPNLPSPGILKPDIIGPGVNILAAWPFPLSNSTDSK 534
BLAST of Bhi12M000319 vs. ExPASy Swiss-Prot
Match:
A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)
HSP 1 Score: 597.4 bits (1539), Expect = 1.5e-169
Identity = 312/517 (60.35%), Postives = 377/517 (72.92%), Query Frame = 0
Query: 9 LLLLLKFHGYEAQLTELPLSSLHTYIVHVKKPE-----TADDLESWHRSFLPTSLENSEE 68
+ LL FH AQ + + L TYIVHV KP+ + DLES++ SFLP ++ SE
Sbjct: 9 IAFLLSFHLTTAQRS----TGLQTYIVHVDKPDAQVLANSADLESYYNSFLPATVSGSEV 68
Query: 69 QPSLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNRQFG 128
+++SY +V +GFAA+L+ E+VK ME+K GFVSA+ EK+L LHTTHTP+FLGL + G
Sbjct: 69 PSRIIHSYHHVATGFAAKLSGEEVKEMEKKAGFVSAKLEKVLTLHTTHTPNFLGLYQNMG 128
Query: 129 FWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPTKWKGRCEFNFSA-CNNKLIGARS 188
FW++SN+GKGVIIG+LD GITP HPSF DV MP PP KWKG+CEF +A CN K+IGAR+
Sbjct: 129 FWQESNYGKGVIIGLLDTGITPGHPSFSDVNMPSPPAKWKGKCEFTGNATCNKKIIGARN 188
Query: 189 FNLASKALKGHATTMDDSPIDEDGHGTHTASTAAGTFIDGAEALGNA---------IAHL 248
F S P DE+GHGTHTASTAAG F++ A GNA +AH+
Sbjct: 189 FISGSGV----------PPTDEEGHGTHTASTAAGNFVNDANVFGNANGTAVGMAPLAHI 248
Query: 249 AIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKGIF 308
A+YKVC + C D DILAALDAA++DGVDVLSLSLG S PF+ D+IAIGAF AI+KGIF
Sbjct: 249 AMYKVCSEDGCSDADILAALDAAIDDGVDVLSLSLGGYSDPFYYDNIAIGAFAAIRKGIF 308
Query: 309 VRCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDFPQT 368
V SA N GP N+TLSNEAPWILTV AST DR+IVA A LGNG++ DGES FQP DFP T
Sbjct: 309 VSASAGNDGPLNSTLSNEAPWILTVGASTHDRKIVATAVLGNGQQYDGESAFQPADFPHT 368
Query: 369 FLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILL 428
LPLV+PG +E A C GSL DVKGKVVVC+RGG +AR+ K VK+AGGAAMIL
Sbjct: 369 LLPLVYPGTSDEEAAFCSSGSLDKFDVKGKVVVCDRGGDVARLEKSQTVKDAGGAAMILA 428
Query: 429 NTESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPKATIVFKGTIIGDDFSPAIAS 488
N E DG T ADAHVLPA+HV + A IK+YINST+ P A I+FKGTIIG SP+++S
Sbjct: 429 NLEIDGEGTFADAHVLPATHVGYAAGEMIKSYINSTSTPTAGILFKGTIIGFKSSPSVSS 488
Query: 489 FSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNT 511
FSSRGP+LASPGI+KPDI GPGV+ILAAWP ++N T
Sbjct: 489 FSSRGPNLASPGIVKPDIIGPGVNILAAWPVSVENKT 511
BLAST of Bhi12M000319 vs. ExPASy Swiss-Prot
Match:
A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)
HSP 1 Score: 566.6 bits (1459), Expect = 2.8e-160
Identity = 305/500 (61.00%), Postives = 364/500 (72.80%), Query Frame = 0
Query: 29 SLHTYIVHVKKPE------TADDLESWHRSFLPTSLENSEEQPSLLYSYRNVMSGFAARL 88
+L TYIVHVKK E + ++L +WH SFLP + + +++SYRNV SGFA RL
Sbjct: 37 NLTTYIVHVKKLEIEGPLQSTEELHTWHHSFLP----ETSNKDRMVFSYRNVASGFAVRL 96
Query: 89 TEEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGG 148
T E+ A++EK+ +S R E+ L LHTTHTP FLGL + G W DSN GKGVIIGV+D G
Sbjct: 97 TPEEANALQEKEEVMSIRPERTLSLHTTHTPSFLGLRQGQGLWNDSNLGKGVIIGVIDTG 156
Query: 149 ITPNHPSFDDVGMPQPPTKWKGRCEF-NFSACNNKLIGARSFNLASKALKGHATTMDDSP 208
I P H SF+D GMP PP KWKG CEF S CNNKLIGAR NL A++ + P
Sbjct: 157 IYPFHLSFNDEGMPPPPAKWKGHCEFTGGSVCNNKLIGAR--NLVKSAIQ-------EPP 216
Query: 209 IDEDGHGTHTASTAAGTFIDGAEALGNA---------IAHLAIYKVCFG---EDCPDTDI 268
++ HGTHTA+ AAG F++GA GNA AHLAIYKVC ++CP++ I
Sbjct: 217 YEDFFHGTHTAAEAAGRFVEGASVFGNARGTAAGMAPDAHLAIYKVCSSKVKDECPESAI 276
Query: 269 LAALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKGIFVRCSAANSGPFNATLS 328
LAA+D A+EDGVDVLSLSLG S PFFED IAIGAF A QKGIFV CSAANSGP ++LS
Sbjct: 277 LAAMDIAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQKGIFVSCSAANSGPHYSSLS 336
Query: 329 NEAPWILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDFPQTFLPLVF-PGEKNETVA 388
NEAPWILTV ASTIDR+I A+AKLGNG E +GE+LFQP DF LPLV+ EKN + A
Sbjct: 337 NEAPWILTVGASTIDRKISASAKLGNGAEYEGETLFQPKDFSSQLLPLVYAAAEKNNSSA 396
Query: 389 LCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNTESDGFTTEADAHV 448
LC GSL+NI+VKGKVVVC+ GGGI IAKG EV +AGG+AMIL N E+ GFTT A+AHV
Sbjct: 397 LCAPGSLRNINVKGKVVVCDLGGGIPFIAKGQEVLDAGGSAMILANIENFGFTTLANAHV 456
Query: 449 LPASHVSHTAALKIKAYINSTTYPKATIVFKGTIIGDDFSPAIASFSSRGPSLASPGILK 508
LPA HVS+ A+L IKAYINST P AT++F+GTIIGD +P++A+FSSRGPS SPGILK
Sbjct: 457 LPAVHVSYAASLAIKAYINSTYTPTATVLFQGTIIGDSLAPSVAAFSSRGPSQQSPGILK 516
BLAST of Bhi12M000319 vs. ExPASy Swiss-Prot
Match:
A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)
HSP 1 Score: 547.4 bits (1409), Expect = 1.8e-154
Identity = 290/506 (57.31%), Postives = 361/506 (71.34%), Query Frame = 0
Query: 21 QLTELPLSSLHTYIVHVKKPET--ADDLESWHRSFLPTSLENSEEQPSLLYSYRNVMSGF 80
+L + S+L TYIVHV+KP+ +DDL +++ S LP S + + ++ ++++YRNV++GF
Sbjct: 33 ELEDEDQSNLSTYIVHVRKPQVIQSDDLHTFYYSLLPESTKTTNQR--IVFTYRNVVNGF 92
Query: 81 AARLTEEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGV 140
A +LT E+ KA+++ + VSAR EKIL LHTTHTP FLGL + G WK SN GKGVIIG+
Sbjct: 93 AVKLTPEEAKALQQNEEVVSARPEKILSLHTTHTPSFLGLQQGLGLWKGSNSGKGVIIGI 152
Query: 141 LDGGITPNHPSFDDVGMPQPPTKWKGRCEFNFS-ACNNKLIGARSFNLASKALKGHATTM 200
LD GI+P HPSF D GMP PP KW G CEF CNNK+IGAR+F +K T+
Sbjct: 153 LDTGISPFHPSFSDEGMPSPPAKWNGICEFTGKRTCNNKIIGARNF------VKTKNLTL 212
Query: 201 DDSPIDEDGHGTHTASTAAGTFIDGAEALGNA---------IAHLAIYKVCFGEDCPDTD 260
P D+ GHGTHTASTAAG + GA GNA AH+A+YKVC C ++
Sbjct: 213 ---PFDDVGHGTHTASTAAGRPVQGANVYGNANGTAVGMAPDAHIAMYKVCGLVGCSESA 272
Query: 261 ILAALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKGIFVRCSAANSGPFNATL 320
ILA +D AV+DGVDVLSLSLG PS PFFED IA+GAF AIQKGIFV CSAANSGP ++L
Sbjct: 273 ILAGMDTAVDDGVDVLSLSLGGPSGPFFEDPIALGAFGAIQKGIFVSCSAANSGPAYSSL 332
Query: 321 SNEAPWILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDFPQTFLPLVFPGE--KNET 380
SNEAPWILTV AS+IDR I+A AKLGNG+E G+S+FQP DF + LPLV+ G N
Sbjct: 333 SNEAPWILTVGASSIDRTIMATAKLGNGKEYVGQSVFQPKDFAPSLLPLVYAGANGNNNF 392
Query: 381 VALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNTESDGFTTEADA 440
C SL DV+GKVV+CE GG + R+ KG VK+AGGAAMIL+N+ + F AD
Sbjct: 393 SVFCAPESLNRSDVEGKVVLCEDGGFVPRVFKGKAVKDAGGAAMILMNSVLEDFNPIADV 452
Query: 441 HVLPASHVSHTAALKIKAYINSTTYPKATIVFKGTIIGDDFSPAIASFSSRGPSLASPGI 500
HVLPA H+S+ A L +K YINST+ P ATI+F+GT+IG+ +P + SFSSRGPS ASPGI
Sbjct: 453 HVLPAVHISYEAGLALKEYINSTSTPTATILFEGTVIGNLLAPQVTSFSSRGPSKASPGI 512
Query: 501 LKPDITGPGVSILAAWPFPLDNNTNP 513
LKPDI GPG++ILAAWP LDN+T P
Sbjct: 513 LKPDIIGPGLNILAAWPVSLDNSTTP 527
BLAST of Bhi12M000319 vs. ExPASy Swiss-Prot
Match:
G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)
HSP 1 Score: 539.3 bits (1388), Expect = 4.8e-152
Identity = 300/523 (57.36%), Postives = 361/523 (69.02%), Query Frame = 0
Query: 7 LFLLLLLKFHGYEAQLTELPLSSLHTYIVHVKKPET-----ADDLESWHRSFLPTSLENS 66
+F+L + E L E +L TYIVHVKK E ++DL SW+ SFLP + +
Sbjct: 13 IFILCSISMLAAEENL-EHDQINLMTYIVHVKKSENVASHQSEDLHSWYHSFLPQTFPHK 72
Query: 67 EEQPSLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNRQ 126
E +++SYR V SGFA +LT E+ K+++EK VSAR E+ L LHTTHTP FLGL +
Sbjct: 73 ER---MVFSYRKVASGFAVKLTPEEAKSLQEKGEIVSARPERTLELHTTHTPTFLGLKQG 132
Query: 127 FGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPTKWKGRCEF-NFSACNNKLIGA 186
G W D N GKGVIIG++D GI P HPSF+D GMP PP KWKG CEF CNNKLIGA
Sbjct: 133 QGLWSDDNLGKGVIIGIIDTGIFPLHPSFNDEGMPPPPAKWKGHCEFTGGQVCNNKLIGA 192
Query: 187 RSFNLASKALKGHATTMDDSPIDEDGHGTHTASTAAGTFIDGAEALGNA---------IA 246
R NL A++ + P + HGTHTA+ AAG FI+ A GNA A
Sbjct: 193 R--NLVKSAIQ-------EPPFENFFHGTHTAAEAAGRFIEDASVFGNAKGVAAGMAPNA 252
Query: 247 HLAIYKVCFGE-DCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQK 306
HLAIYKVC + C ++ ILAA+D A+EDGVDVLSLSLG S PFFED IAIGAF A Q
Sbjct: 253 HLAIYKVCNDKIGCTESAILAAMDIAIEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQN 312
Query: 307 GIFVRCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDF 366
G+FV CSAANSGP +TLSNEAPWILTV ASTIDR+IVA+AKLGNGEE +GE+LFQP DF
Sbjct: 313 GVFVSCSAANSGPGYSTLSNEAPWILTVGASTIDRKIVASAKLGNGEEYEGETLFQPKDF 372
Query: 367 PQTFLPLVFP-----GEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNA 426
Q LPLV+P G + + +LC GSLKNID+ GKVV+C+ G ++ I KG EV N+
Sbjct: 373 SQQLLPLVYPGSFGYGNQTQNQSLCLPGSLKNIDLSGKVVLCD-VGNVSSIVKGQEVLNS 432
Query: 427 GGAAMILLNTESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPKATIVFKGTIIGD 486
GG AMIL N+E+ GF+T A AHVLPA VS+ A L IK+YI ST P AT++FKGTIIGD
Sbjct: 433 GGIAMILANSEALGFSTFAIAHVLPAVEVSYAAGLTIKSYIKSTYNPTATLIFKGTIIGD 492
Query: 487 DFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDN 509
+P++ FSSRGPS SPGILKPDI GPGV+ILAAW +DN
Sbjct: 493 SLAPSVVYFSSRGPSQESPGILKPDIIGPGVNILAAWAVSVDN 521
BLAST of Bhi12M000319 vs. NCBI nr
Match:
XP_038876087.1 (LOW QUALITY PROTEIN: subtilisin-like protease 4 [Benincasa hispida])
HSP 1 Score: 964.9 bits (2493), Expect = 2.7e-277
Identity = 487/499 (97.60%), Postives = 488/499 (97.80%), Query Frame = 0
Query: 24 ELPLSSLHTYIVHVKKPETADDLESWHRSFLPTSLENSEEQPSLLYSYRNVMSGFAARLT 83
+LPL LHTYIVHVKKPETADDLESWHRSFLPTSLENSEEQPSLLYSYRNVMSGFAARLT
Sbjct: 23 KLPL-CLHTYIVHVKKPETADDLESWHRSFLPTSLENSEEQPSLLYSYRNVMSGFAARLT 82
Query: 84 EEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGI 143
EEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGI
Sbjct: 83 EEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNRQFGFWKDSNFGKGVIIGVLDGGI 142
Query: 144 TPNHPSFDDVGMPQPPTKWKGRCEFNFSACNNKLIGARSFNLASKALKGHATTMDDSPID 203
TPNHPSFDDVGMPQPPTKWKGRCEFNFSACNNKLIGARSFNLASKALKGHATTMDDSPID
Sbjct: 143 TPNHPSFDDVGMPQPPTKWKGRCEFNFSACNNKLIGARSFNLASKALKGHATTMDDSPID 202
Query: 204 EDGHGTHTASTAAGTFIDGAEALGNAI---------AHLAIYKVCFGEDCPDTDILAALD 263
EDGHGTHTASTAAGTFIDGAEALGNAI AHLAIYKVCFGEDCPDTDILAALD
Sbjct: 203 EDGHGTHTASTAAGTFIDGAEALGNAIGTAXGMAPLAHLAIYKVCFGEDCPDTDILAALD 262
Query: 264 AAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKGIFVRCSAANSGPFNATLSNEAPW 323
AAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKGIFVRCSAANSGPFNATLSNEAPW
Sbjct: 263 AAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKGIFVRCSAANSGPFNATLSNEAPW 322
Query: 324 ILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDFPQTFLPLVFPGEKNETVALCGEGS 383
ILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDFPQTFLPLVFPGEKNETVALCGEGS
Sbjct: 323 ILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDFPQTFLPLVFPGEKNETVALCGEGS 382
Query: 384 LKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNTESDGFTTEADAHVLPASHV 443
LKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNTESDGFTTEADAHVLPASHV
Sbjct: 383 LKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMILLNTESDGFTTEADAHVLPASHV 442
Query: 444 SHTAALKIKAYINSTTYPKATIVFKGTIIGDDFSPAIASFSSRGPSLASPGILKPDITGP 503
SHTAALKIKAYINSTTYPKATIVFKGTIIGDDFSPAIASFSSRGPSLASPGILKPDITGP
Sbjct: 443 SHTAALKIKAYINSTTYPKATIVFKGTIIGDDFSPAIASFSSRGPSLASPGILKPDITGP 502
Query: 504 GVSILAAWPFPLDNNTNPK 514
GVSILAAWPFPLDNNTNPK
Sbjct: 503 GVSILAAWPFPLDNNTNPK 520
BLAST of Bhi12M000319 vs. NCBI nr
Match:
KAA0042818.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])
HSP 1 Score: 931.8 bits (2407), Expect = 2.6e-267
Identity = 465/522 (89.08%), Postives = 480/522 (91.95%), Query Frame = 0
Query: 1 MVVLPSLFLLLLLKFHGYEAQLTELPLSSLHTYIVHVKKPETADDLESWHRSFLPTSLEN 60
MV+LPSLFLLLLL FHGYEAQ+TELPLS+LHTYIVHVKKPE DDLE WHRSFLPTSL+N
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 SEEQPSLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNR 120
EEQP+LLYSYRNVMSGF+ARLTEE VKAMEEKDGFVSARRE I+HLHTTHTPDFLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPTKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDD GMPQPP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLASKALKGHATTMDDSPIDEDGHGTHTASTAAGTFIDGAEALGNA---------IA 240
RS NLAS+ALKG TT+DDSPIDEDGHGTHTASTAAGTF+DGAEALGNA +A
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKG 300
HLAIYKVCFGEDC D DILA LDAAVEDGVDVLS+SLG PS PFF D AIG+F AIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVRCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDFP 360
IFV CSAANSGPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEE DGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
QT LPLVFPGEKNETVALC EGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN GGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNTESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPKATIVFKGTIIGDDFSPAI 480
LLN ESDGFTTE DAHVLPASHVSHTAALKIKAYINSTTYP ATI+FKGT IGDDFSPAI
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNPK 514
ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTN K
Sbjct: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTK 522
BLAST of Bhi12M000319 vs. NCBI nr
Match:
XP_008437181.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])
HSP 1 Score: 928.7 bits (2399), Expect = 2.2e-266
Identity = 464/522 (88.89%), Postives = 479/522 (91.76%), Query Frame = 0
Query: 1 MVVLPSLFLLLLLKFHGYEAQLTELPLSSLHTYIVHVKKPETADDLESWHRSFLPTSLEN 60
MV+LPSLFLLLLL FHGYEAQ+TELPLS+LHTYIVHVKKPE DDLE WHRSFLPTSL+N
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 SEEQPSLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNR 120
EEQP+LLYSYRNVMSGF+ARLTEE VKAMEEKDGFVSARRE I+HLHTTHTPDFLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPTKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDD GM QPP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMAQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLASKALKGHATTMDDSPIDEDGHGTHTASTAAGTFIDGAEALGNA---------IA 240
RS NLAS+ALKG TT+DDSPIDEDGHGTHTASTAAGTF+DGAEALGNA +A
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKG 300
HLAIYKVCFGEDC D DILA LDAAVEDGVDVLS+SLG PS PFF D AIG+F AIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVRCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDFP 360
IFV CSAANSGPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEE DGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
QT LPLVFPGEKNETVALC EGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN GGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNTESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPKATIVFKGTIIGDDFSPAI 480
LLN ESDGFTTE DAHVLPASHVSHTAALKIKAYINSTTYP ATI+FKGT IGDDFSPAI
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNPK 514
ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTN K
Sbjct: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTK 522
BLAST of Bhi12M000319 vs. NCBI nr
Match:
KGN50165.1 (hypothetical protein Csa_000664 [Cucumis sativus])
HSP 1 Score: 922.2 bits (2382), Expect = 2.0e-264
Identity = 462/522 (88.51%), Postives = 480/522 (91.95%), Query Frame = 0
Query: 1 MVVLPSLFLLLLLKFHGYEAQLTELPLSSLHTYIVHVKKPETADDLESWHRSFLPTSLEN 60
MV+LPSLFLLLLL FH YEAQ+TELP S+LHTYIVHVKKPE DDLESWHRSFLPTSLEN
Sbjct: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60
Query: 61 SEEQPSLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNR 120
SEEQP+LLYSYRNVMSGF+ARLTEE VKAMEEKDGFVSARRE I+HLHTTH+P+FLGLNR
Sbjct: 61 SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPTKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITP+HPSF D GMPQPP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLASKALKGHATTMDDSPIDEDGHGTHTASTAAGTFIDGAEALGNA---------IA 240
RS NLAS+ALKG TT+DDSPIDEDGHGTHTASTAAGTF+DGAEALGNA +A
Sbjct: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKG 300
HLAIYKVCFGE C + DILA LDAAVEDGVDVLS+SLG P PFF D AIGAF AIQKG
Sbjct: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300
Query: 301 IFVRCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDFP 360
IFV CSAANSGPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEE DGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
QTFLPLVFPGEKNETVALC EGSLKNIDVKGKVVVC+RGGGIARIAKGVEVKNAGGAAMI
Sbjct: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420
Query: 421 LLNTESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPKATIVFKGTIIGDDFSPAI 480
LLN ESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYP ATIVFKGT IGDDFSPAI
Sbjct: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNPK 514
A+FSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTN K
Sbjct: 481 AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTK 522
BLAST of Bhi12M000319 vs. NCBI nr
Match:
XP_023532974.1 (subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 871.7 bits (2251), Expect = 3.1e-249
Identity = 444/523 (84.89%), Postives = 468/523 (89.48%), Query Frame = 0
Query: 1 MVVLPSLFLLLLLKFHGYEAQLTELPLSSLHTYIVHVKKPETADDLESWHRSFLPTSLEN 60
MV PSLFLLLLL FH A +TELPL +L TYIVHV+KPET DDLESWHRSFLP+S
Sbjct: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVEKPETTDDLESWHRSFLPSS--- 60
Query: 61 SEEQPSLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNR 120
SLLYSYRNVMSGFAARL+EEQVKAMEEKDGFVSARRE+IL LHTTHTPDFLGLNR
Sbjct: 61 ----SSLLYSYRNVMSGFAARLSEEQVKAMEEKDGFVSARRERILQLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPTKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGI P+HPSFDDVGMP PP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLASKALKGHATTMDDSPIDEDGHGTHTASTAAGTFIDGAEALGNA---------IA 240
RSFNLA+K LKG T MDDSPIDEDGHGTHTASTAAG F+ GAEALGNA +A
Sbjct: 181 RSFNLATKVLKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKG 300
HLAIYKVCFGEDCPDTDILAALDAA+EDGVDVLSLSLGSPS PFF+D +AIGAF AIQKG
Sbjct: 241 HLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKG 300
Query: 301 IFVRCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDFP 360
IFV CSAANSGPF ATLSNEAPWILTVAASTIDRRI AAAKLGNGEE DGESLFQP+DFP
Sbjct: 301 IFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFP 360
Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
TFLPLV+ GEKN+T ALCGEGSLK+IDVKGK+VVCERGGGIARIAKG EVKNAGGAAMI
Sbjct: 361 PTFLPLVYAGEKNQTAALCGEGSLKDIDVKGKIVVCERGGGIARIAKGTEVKNAGGAAMI 420
Query: 421 LLNTESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPKATIVFKGTIIGDD-FSPA 480
LLN + DGF+TEADAHVLPASHVSH AALKIKAYINSTTYP ATI+FKGT+IGDD FSPA
Sbjct: 421 LLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPA 480
Query: 481 IASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNPK 514
IASFSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N+N K
Sbjct: 481 IASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNSNTK 515
BLAST of Bhi12M000319 vs. ExPASy TrEMBL
Match:
A0A5A7TJ55 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003250 PE=3 SV=1)
HSP 1 Score: 931.8 bits (2407), Expect = 1.2e-267
Identity = 465/522 (89.08%), Postives = 480/522 (91.95%), Query Frame = 0
Query: 1 MVVLPSLFLLLLLKFHGYEAQLTELPLSSLHTYIVHVKKPETADDLESWHRSFLPTSLEN 60
MV+LPSLFLLLLL FHGYEAQ+TELPLS+LHTYIVHVKKPE DDLE WHRSFLPTSL+N
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 SEEQPSLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNR 120
EEQP+LLYSYRNVMSGF+ARLTEE VKAMEEKDGFVSARRE I+HLHTTHTPDFLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPTKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDD GMPQPP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLASKALKGHATTMDDSPIDEDGHGTHTASTAAGTFIDGAEALGNA---------IA 240
RS NLAS+ALKG TT+DDSPIDEDGHGTHTASTAAGTF+DGAEALGNA +A
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKG 300
HLAIYKVCFGEDC D DILA LDAAVEDGVDVLS+SLG PS PFF D AIG+F AIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVRCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDFP 360
IFV CSAANSGPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEE DGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
QT LPLVFPGEKNETVALC EGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN GGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNTESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPKATIVFKGTIIGDDFSPAI 480
LLN ESDGFTTE DAHVLPASHVSHTAALKIKAYINSTTYP ATI+FKGT IGDDFSPAI
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNPK 514
ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTN K
Sbjct: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTK 522
BLAST of Bhi12M000319 vs. ExPASy TrEMBL
Match:
A0A1S3ATZ7 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482686 PE=3 SV=1)
HSP 1 Score: 928.7 bits (2399), Expect = 1.0e-266
Identity = 464/522 (88.89%), Postives = 479/522 (91.76%), Query Frame = 0
Query: 1 MVVLPSLFLLLLLKFHGYEAQLTELPLSSLHTYIVHVKKPETADDLESWHRSFLPTSLEN 60
MV+LPSLFLLLLL FHGYEAQ+TELPLS+LHTYIVHVKKPE DDLE WHRSFLPTSL+N
Sbjct: 1 MVLLPSLFLLLLLNFHGYEAQVTELPLSNLHTYIVHVKKPEVVDDLEIWHRSFLPTSLDN 60
Query: 61 SEEQPSLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNR 120
EEQP+LLYSYRNVMSGF+ARLTEE VKAMEEKDGFVSARRE I+HLHTTHTPDFLGLNR
Sbjct: 61 EEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPTKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDD GM QPP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDAGMAQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLASKALKGHATTMDDSPIDEDGHGTHTASTAAGTFIDGAEALGNA---------IA 240
RS NLAS+ALKG TT+DDSPIDEDGHGTHTASTAAGTF+DGAEALGNA +A
Sbjct: 181 RSMNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKG 300
HLAIYKVCFGEDC D DILA LDAAVEDGVDVLS+SLG PS PFF D AIG+F AIQKG
Sbjct: 241 HLAIYKVCFGEDCSDVDILAGLDAAVEDGVDVLSISLGGPSVPFFADITAIGSFAAIQKG 300
Query: 301 IFVRCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDFP 360
IFV CSAANSGPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEE DGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
QT LPLVFPGEKNETVALC EGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN GGAAMI
Sbjct: 361 QTLLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNGGGAAMI 420
Query: 421 LLNTESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPKATIVFKGTIIGDDFSPAI 480
LLN ESDGFTTE DAHVLPASHVSHTAALKIKAYINSTTYP ATI+FKGT IGDDFSPAI
Sbjct: 421 LLNAESDGFTTEVDAHVLPASHVSHTAALKIKAYINSTTYPTATILFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNPK 514
ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTN K
Sbjct: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTK 522
BLAST of Bhi12M000319 vs. ExPASy TrEMBL
Match:
A0A0A0KKE3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157240 PE=3 SV=1)
HSP 1 Score: 922.2 bits (2382), Expect = 9.8e-265
Identity = 462/522 (88.51%), Postives = 480/522 (91.95%), Query Frame = 0
Query: 1 MVVLPSLFLLLLLKFHGYEAQLTELPLSSLHTYIVHVKKPETADDLESWHRSFLPTSLEN 60
MV+LPSLFLLLLL FH YEAQ+TELP S+LHTYIVHVKKPE DDLESWHRSFLPTSLEN
Sbjct: 1 MVLLPSLFLLLLLNFHVYEAQVTELPFSNLHTYIVHVKKPEVVDDLESWHRSFLPTSLEN 60
Query: 61 SEEQPSLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNR 120
SEEQP+LLYSYRNVMSGF+ARLTEE VKAMEEKDGFVSARRE I+HLHTTH+P+FLGLNR
Sbjct: 61 SEEQPTLLYSYRNVMSGFSARLTEEHVKAMEEKDGFVSARRETIVHLHTTHSPNFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPTKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGITP+HPSF D GMPQPP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGITPSHPSFVDAGMPQPPAKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLASKALKGHATTMDDSPIDEDGHGTHTASTAAGTFIDGAEALGNA---------IA 240
RS NLAS+ALKG TT+DDSPIDEDGHGTHTASTAAGTF+DGAEALGNA +A
Sbjct: 181 RSLNLASQALKGKITTLDDSPIDEDGHGTHTASTAAGTFVDGAEALGNAFGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKG 300
HLAIYKVCFGE C + DILA LDAAVEDGVDVLS+SLG P PFF D AIGAF AIQKG
Sbjct: 241 HLAIYKVCFGESCSNVDILAGLDAAVEDGVDVLSISLGGPPVPFFADITAIGAFAAIQKG 300
Query: 301 IFVRCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDFP 360
IFV CSAANSGPFNATLSNEAPWILTVAASTIDR+I A AKLGNGEE DGESLFQPNDFP
Sbjct: 301 IFVSCSAANSGPFNATLSNEAPWILTVAASTIDRKITATAKLGNGEEFDGESLFQPNDFP 360
Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
QTFLPLVFPGEKNETVALC EGSLKNIDVKGKVVVC+RGGGIARIAKGVEVKNAGGAAMI
Sbjct: 361 QTFLPLVFPGEKNETVALCAEGSLKNIDVKGKVVVCDRGGGIARIAKGVEVKNAGGAAMI 420
Query: 421 LLNTESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPKATIVFKGTIIGDDFSPAI 480
LLN ESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYP ATIVFKGT IGDDFSPAI
Sbjct: 421 LLNAESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPTATIVFKGTTIGDDFSPAI 480
Query: 481 ASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNPK 514
A+FSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTN K
Sbjct: 481 AAFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNTK 522
BLAST of Bhi12M000319 vs. ExPASy TrEMBL
Match:
A0A6J1H242 (subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111459745 PE=3 SV=1)
HSP 1 Score: 867.5 bits (2240), Expect = 2.9e-248
Identity = 444/523 (84.89%), Postives = 465/523 (88.91%), Query Frame = 0
Query: 1 MVVLPSLFLLLLLKFHGYEAQLTELPLSSLHTYIVHVKKPETADDLESWHRSFLPTSLEN 60
MV PSLFLLLLL FH A +TELPL +L TYIVHVKKPET DDLE WHRSFLP+S
Sbjct: 1 MVAFPSLFLLLLLNFHYVGALVTELPLINLQTYIVHVKKPETTDDLEIWHRSFLPSS--- 60
Query: 61 SEEQPSLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARREKILHLHTTHTPDFLGLNR 120
SLLYSYRNVMSGFAARL+EEQVKAMEE DGFVSARRE+IL LHTTHTPDFLGLNR
Sbjct: 61 ----SSLLYSYRNVMSGFAARLSEEQVKAMEEMDGFVSARRERILQLHTTHTPDFLGLNR 120
Query: 121 QFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPTKWKGRCEFNFSACNNKLIGA 180
QFGFWKDSNFGKGVIIGVLDGGI P+HPSFDDVGMP PP KWKGRCEFNFSACNNKLIGA
Sbjct: 121 QFGFWKDSNFGKGVIIGVLDGGIAPSHPSFDDVGMPPPPPKWKGRCEFNFSACNNKLIGA 180
Query: 181 RSFNLASKALKGHATTMDDSPIDEDGHGTHTASTAAGTFIDGAEALGNA---------IA 240
RSFNLA+KALKG T MDDSPIDEDGHGTHTASTAAG F+ GAEALGNA +A
Sbjct: 181 RSFNLATKALKGE-TMMDDSPIDEDGHGTHTASTAAGAFVKGAEALGNAKGTAVGMAPLA 240
Query: 241 HLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAFTAIQKG 300
HLAIYKVCFGEDCPDTDILAALDAA+EDGVDVLSLSLGSPS PFF+D +AIGAF AIQKG
Sbjct: 241 HLAIYKVCFGEDCPDTDILAALDAAIEDGVDVLSLSLGSPSVPFFQDLVAIGAFAAIQKG 300
Query: 301 IFVRCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEELDGESLFQPNDFP 360
IFV CSAANSGPF ATLSNEAPWILTVAASTIDRRI AAAKLGNGEE DGESLFQP+DFP
Sbjct: 301 IFVSCSAANSGPFKATLSNEAPWILTVAASTIDRRIKAAAKLGNGEEFDGESLFQPSDFP 360
Query: 361 QTFLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKNAGGAAMI 420
T LPLV+ GEKN+T ALCGEGSLK+IDVKGKVVVCERGGGIARIAKG EVKNAGGAAMI
Sbjct: 361 PTLLPLVYAGEKNQTAALCGEGSLKDIDVKGKVVVCERGGGIARIAKGTEVKNAGGAAMI 420
Query: 421 LLNTESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPKATIVFKGTIIGDD-FSPA 480
LLN + DGF+TEADAHVLPASHVSH AALKIKAYINSTTYP ATI+FKGT+IGDD FSPA
Sbjct: 421 LLNQQQDGFSTEADAHVLPASHVSHKAALKIKAYINSTTYPTATILFKGTVIGDDNFSPA 480
Query: 481 IASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNTNPK 514
IASFSSRGPS+ASPGILKPDITGPGVSILAAWPFPLD N N K
Sbjct: 481 IASFSSRGPSVASPGILKPDITGPGVSILAAWPFPLDKNRNTK 515
BLAST of Bhi12M000319 vs. ExPASy TrEMBL
Match:
A0A6J1E0E3 (subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111025997 PE=3 SV=1)
HSP 1 Score: 833.6 bits (2152), Expect = 4.6e-238
Identity = 430/527 (81.59%), Postives = 458/527 (86.91%), Query Frame = 0
Query: 1 MVVLPSLFLLLLLKFHGYEAQLTELPLSSLHTYIVHVKKPE------TADDLESWHRSFL 60
MVVLPSLFLL+L FHG A+ T S+L TYIVHVKKPE +DLE+WHRSFL
Sbjct: 1 MVVLPSLFLLVLFHFHGGGARPTS---SNLQTYIVHVKKPEKLSTYGQVEDLENWHRSFL 60
Query: 61 PTSLENSEEQPSLLYSYRNVMSGFAARLTEEQVKAMEEKDGFVSARREKILHLHTTHTPD 120
P+S SE+ P LLYS+ NVMSGFAARLTEE VKAMEEKDGF+SARRE+IL LHTTHTP+
Sbjct: 61 PSS---SEDHPKLLYSFXNVMSGFAARLTEEHVKAMEEKDGFISARRERILKLHTTHTPN 120
Query: 121 FLGLNRQFGFWKDSNFGKGVIIGVLDGGITPNHPSFDDVGMPQPPTKWKGRCEFNFSACN 180
FLGLNRQFGFWKDSNFGKG IIGVLDGGITPNHPSFDD GMP PP KWKGRCEFNFSACN
Sbjct: 121 FLGLNRQFGFWKDSNFGKGXIIGVLDGGITPNHPSFDDAGMPPPPVKWKGRCEFNFSACN 180
Query: 181 NKLIGARSFNLASKALKGHATTMDDSPIDEDGHGTHTASTAAGTFIDGAEALGNA----- 240
NKLIGARSFNLA+KALKG T DSPIDEDGHGTHTASTAAG F+D AEALGNA
Sbjct: 181 NKLIGARSFNLATKALKGEQPTATDSPIDEDGHGTHTASTAAGAFVDEAEALGNAKGTAV 240
Query: 241 ----IAHLAIYKVCFGEDCPDTDILAALDAAVEDGVDVLSLSLGSPSAPFFEDSIAIGAF 300
+AHLAIYKVCFGEDCPD+DILAALDAAVEDGVDVLSLSLGS SAPFF D IAIGAF
Sbjct: 241 GMAPLAHLAIYKVCFGEDCPDSDILAALDAAVEDGVDVLSLSLGSDSAPFFRDDIAIGAF 300
Query: 301 TAIQKGIFVRCSAANSGPFNATLSNEAPWILTVAASTIDRRIVAAAKLGNGEELDGESLF 360
A QKGIFV CSA+NSGP NATLSNEAPWILTVAASTIDRRI+A AKLGNGEE DGESLF
Sbjct: 301 AATQKGIFVSCSASNSGPLNATLSNEAPWILTVAASTIDRRIIATAKLGNGEEFDGESLF 360
Query: 361 QPNDFPQT-FLPLVFPGEKNETVALCGEGSLKNIDVKGKVVVCERGGGIARIAKGVEVKN 420
QP+DFP T FLPLV+ GEKNET ALCGEGSLK+IDVKGK VVCERGGGIARIAKG+EVKN
Sbjct: 361 QPSDFPPTLFLPLVYAGEKNETAALCGEGSLKDIDVKGKAVVCERGGGIARIAKGMEVKN 420
Query: 421 AGGAAMILLNTESDGFTTEADAHVLPASHVSHTAALKIKAYINSTTYPKATIVFKGTIIG 480
AGGAAMILLN ++DGFTTEADAHVLPA+HV+H AALKIK YINST P A I F+GT+IG
Sbjct: 421 AGGAAMILLNQQTDGFTTEADAHVLPATHVNHVAALKIKDYINSTHNPIAAIAFRGTVIG 480
Query: 481 -DDFSPAIASFSSRGPSLASPGILKPDITGPGVSILAAWPFPLDNNT 511
DDFSPAIASFSSRGPSLASPGILKPDITGPG+SILAAWPFPLDNNT
Sbjct: 481 DDDFSPAIASFSSRGPSLASPGILKPDITGPGISILAAWPFPLDNNT 521
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| A9QY38 | 1.2e-190 | 64.27 | Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1 | [more] |
| A0A0M3R8G2 | 1.5e-169 | 60.35 | Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1 | [more] |
| A9QY40 | 2.8e-160 | 61.00 | Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1 | [more] |
| A9QY39 | 1.8e-154 | 57.31 | Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1 | [more] |
| G7KEU7 | 4.8e-152 | 57.36 | Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_038876087.1 | 2.7e-277 | 97.60 | LOW QUALITY PROTEIN: subtilisin-like protease 4 [Benincasa hispida] | [more] |
| KAA0042818.1 | 2.6e-267 | 89.08 | subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa] | [more] |
| XP_008437181.1 | 2.2e-266 | 88.89 | PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo] | [more] |
| KGN50165.1 | 2.0e-264 | 88.51 | hypothetical protein Csa_000664 [Cucumis sativus] | [more] |
| XP_023532974.1 | 3.1e-249 | 84.89 | subtilisin-like protease SBT1.8 [Cucurbita pepo subsp. pepo] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A5A7TJ55 | 1.2e-267 | 89.08 | Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... | [more] |
| A0A1S3ATZ7 | 1.0e-266 | 88.89 | subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482686 PE=3 SV=... | [more] |
| A0A0A0KKE3 | 9.8e-265 | 88.51 | Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157240 PE=3 SV=1 | [more] |
| A0A6J1H242 | 2.9e-248 | 84.89 | subtilisin-like protease SBT1.8 OS=Cucurbita moschata OX=3662 GN=LOC111459745 PE... | [more] |
| A0A6J1E0E3 | 4.6e-238 | 81.59 | subtilisin-like protease SBT1.7 OS=Momordica charantia OX=3673 GN=LOC111025997 P... | [more] |
