Bhi12M000228 (mRNA) Wax gourd (B227) v1

Overview


Name	Bhi12M000228
Type	mRNA
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	protein LURP-one-related 4
Location	chr12: 7401067 .. 7402224 (-)
Sequence length	944
RNA-Seq Expression	Bhi12M000228
Synteny	Bhi12M000228

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAGAAGAAGAAAAAAAAAAAAAGAAAAACTCCCTATAAATTCCCCTATCTCTCATTTCTACCTTCTTCATCAAACTTTTAAACCTTATTCTCTCTCTTCCATCTCAATGGCCAAAGTGTACCCTCAAACCTTACCGTCGCCGTCGCCGTCATCATCTCCGTCGTCTTCATCGCTCTGTTCCGAAACCGAAAGCGAAACCTTCACAATTTGGATGAAATCTCTGATTTACCACACAAATGGCTGCACCGTCTTCGATTCCAATGGCGACATCGTCTACAGAGTCGATAACTACGACAGAAAATGCAGTCGGGAAGTATTTCTCATGGATCTCAGAGGAAAAGTTCTGTTCACCATCAGAAAAAAGGTTAGCCAAGCCACAAACAAAACTAACAAAAATTAGCGGTTTCTTCGATATTCTTCCTCTTGATTTCGATGTTAAATCATCTAGTTCTGTTGGATTAATCATAGATGTGAATAAATTTCTGCAGAAATTCTCGATTCTTGGAGGATGGGAAGGATTTCGATGGATCGAATCGGAGAGAAGTAAGAAGGCTAGCTTTGGAGTGAAGAAACTGTACAGAACAGCTTCGAGGAAGAGCAATCAATCGGTATGTGAAATAAATGTGGGATTTGAGAGATTTTCATTGGTGAAAATGGATGGGAAACTGGCTTTCAGAATAATCAACATCAATGGAGAAGTAGTTGCAGAGGTAATTATAAATCTGAATCTGAATCTGAATCTGAATCTGAATTTGGTAGAAGCGGATTTGAAAATAATTGATTTGATTGTGTTATTGCAGGCGAAAAGAAAAGTATCATGGAACGGAATATTATTGGGAGATGATGTGTTGAGTTTAAATGCAAATTCTCAGATTGAAAGATCTTTGATTATGGCTTTGGTCACTGTTTATGGCTTAATCCGTCGCCAAATGTAAAATTAAATTATAAAGAGGAAAAAATGAGGAAAAAAAACAGAGAGTTTATACAAATTTTCCTTTTTTTTTTTTTTTTTGGTTGAGGATTTTGTGATTTGTATAGAACTTTCCGGATTTTGGTTTCTTGTAAAGGATTAGTGTAGAAGTTACGAAGGAATACAGATATCAATTTCATTCATTAATTCTTCTTCTTTATTATATTATTTTATTTTTCTGGTTTAA

mRNA sequence

GAAGAAGAAGAAAAAAAAAAAAAGAAAAACTCCCTATAAATTCCCCTATCTCTCATTTCTACCTTCTTCATCAAACTTTTAAACCTTATTCTCTCTCTTCCATCTCAATGGCCAAAGTGTACCCTCAAACCTTACCGTCGCCGTCGCCGTCATCATCTCCGTCGTCTTCATCGCTCTGTTCCGAAACCGAAAGCGAAACCTTCACAATTTGGATGAAATCTCTGATTTACCACACAAATGGCTGCACCGTCTTCGATTCCAATGGCGACATCGTCTACAGAGTCGATAACTACGACAGAAAATGCAGTCGGGAAGTATTTCTCATGGATCTCAGAGGAAAAGTTCTGTTCACCATCAGAAAAAAGAAATTCTCGATTCTTGGAGGATGGGAAGGATTTCGATGGATCGAATCGGAGAGAAGTAAGAAGGCTAGCTTTGGAGTGAAGAAACTGTACAGAACAGCTTCGAGGAAGAGCAATCAATCGGTATGTGAAATAAATGTGGGATTTGAGAGATTTTCATTGGTGAAAATGGATGGGAAACTGGCTTTCAGAATAATCAACATCAATGGAGAAGTAGTTGCAGAGGCGAAAAGAAAAGTATCATGGAACGGAATATTATTGGGAGATGATGTGTTGAGTTTAAATGCAAATTCTCAGATTGAAAGATCTTTGATTATGGCTTTGGTCACTGTTTATGGCTTAATCCGTCGCCAAATGTAAAATTAAATTATAAAGAGGAAAAAATGAGGAAAAAAAACAGAGAGTTTATACAAATTTTCCTTTTTTTTTTTTTTTTTGGTTGAGGATTTTGTGATTTGTATAGAACTTTCCGGATTTTGGTTTCTTGTAAAGGATTAGTGTAGAAGTTACGAAGGAATACAGATATCAATTTCATTCATTAATTCTTCTTCTTTATTATATTATTTTATTTTTCTGGTTTAA

Coding sequence (CDS)

ATGGCCAAAGTGTACCCTCAAACCTTACCGTCGCCGTCGCCGTCATCATCTCCGTCGTCTTCATCGCTCTGTTCCGAAACCGAAAGCGAAACCTTCACAATTTGGATGAAATCTCTGATTTACCACACAAATGGCTGCACCGTCTTCGATTCCAATGGCGACATCGTCTACAGAGTCGATAACTACGACAGAAAATGCAGTCGGGAAGTATTTCTCATGGATCTCAGAGGAAAAGTTCTGTTCACCATCAGAAAAAAGAAATTCTCGATTCTTGGAGGATGGGAAGGATTTCGATGGATCGAATCGGAGAGAAGTAAGAAGGCTAGCTTTGGAGTGAAGAAACTGTACAGAACAGCTTCGAGGAAGAGCAATCAATCGGTATGTGAAATAAATGTGGGATTTGAGAGATTTTCATTGGTGAAAATGGATGGGAAACTGGCTTTCAGAATAATCAACATCAATGGAGAAGTAGTTGCAGAGGCGAAAAGAAAAGTATCATGGAACGGAATATTATTGGGAGATGATGTGTTGAGTTTAAATGCAAATTCTCAGATTGAAAGATCTTTGATTATGGCTTTGGTCACTGTTTATGGCTTAATCCGTCGCCAAATGTAA

Protein sequence

MAKVYPQTLPSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVDNYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRTASRKSNQSVCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLSLNANSQIERSLIMALVTVYGLIRRQM

Homology

[Close]

BLAST of Bhi12M000228 vs. TAIR 10
Match: AT1G63410.1 (Protein of unknown function (DUF567) )

HSP 1 Score: 156.0 bits (393), Expect = 3.3e-38
Identity = 92/203 (45.32%), Postives = 131/203 (64.53%), Query Frame = 0


Query: 1   MAKVYPQTLPSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD 60
           MA+V+PQ     +  SSP  S     TE ETFT+WMKSL+Y TNG TV++SNG+I YRV+
Sbjct: 1   MARVFPQ-----AAISSPYMS-----TERETFTVWMKSLVYQTNGLTVYNSNGEITYRVE 60

Query: 61  NYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRTAS 120
           NYD KCS EV +MDL G +LFTIRKKK  + G W  +R   S  S +      ++ R++ 
Sbjct: 61  NYD-KCSNEVHIMDLHGNILFTIRKKKLWLFGSWYVYRECGSFTSTEEVKPCARIKRSSI 120

Query: 121 RKSNQSV-CEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLSL 180
           R  +  V  E N   E F +++ D K AF+II+I+G ++A+ K K S NGI LG+DVL+L
Sbjct: 121 RDGDWEVRDETN---EVFWILRFDPKFAFQIIDIHGNIIAQVKPKQSSNGITLGEDVLTL 180

Query: 181 NANSQIERSLIMALVTVYGLIRR 203
               +++ SL++ LVT+ G +++
Sbjct: 181 EVKPRVDHSLVVTLVTIDGEVKQ 189

[Close]

BLAST of Bhi12M000228 vs. TAIR 10
Match: AT3G14260.1 (Protein of unknown function (DUF567) )

HSP 1 Score: 149.1 bits (375), Expect = 4.0e-36
Identity = 92/210 (43.81%), Postives = 126/210 (60.00%), Query Frame = 0


Query: 1   MAKVYPQTLPSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD 60
           M K++P    S    +   +SS    TE E+FTIWMKSL+++TNGCTVFDS G+I+YRVD
Sbjct: 23  MVKIHPDLTTS---GAGEETSSPYLTTEQESFTIWMKSLVFNTNGCTVFDSKGNIIYRVD 82

Query: 61  NYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFR----WIESERSKKASFGVKKLY 120
           NY+ K  REV+LMDL G VLFT+R++KF +   WEG+R     +ES  +K   F VK   
Sbjct: 83  NYNSKSCREVYLMDLSGHVLFTLRRQKFGLFKTWEGYRSSSATVES-TTKLEYFRVKNNV 142

Query: 121 RTASRKSNQSVCEINVGFER------FSLVKMDGKLAFRIININGEVVAEAKRKVSWNGI 180
                K + S   +N G  R      + +V     LA  I +  G+++AE KRK S NG+
Sbjct: 143 FQVPNKDSSSSYRVNAGSCRNDEQYCYKMVTRGSSLA--IEDNCGKLLAEVKRKQSINGL 202

Query: 181 LLGDDVLSLNANSQIERSLIMALVTVYGLI 201
            LGDDVL++   SQ++ S I+ LV  + LI
Sbjct: 203 KLGDDVLTMMVESQVDHSFIIGLVLAHSLI 226

[Close]

BLAST of Bhi12M000228 vs. TAIR 10
Match: AT1G53890.1 (Protein of unknown function (DUF567) )

HSP 1 Score: 132.9 bits (333), Expect = 2.9e-31
Identity = 76/186 (40.86%), Postives = 115/186 (61.83%), Query Frame = 0


Query: 27  TESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVDNYDRKC-SREVFLMDLRGKVLFTIRK 86
           TE E FTIWMKSL++++ GCTVFDS G+++YRVDNYD K  S EV+ MDL GK+LFT+R+
Sbjct: 39  TEQERFTIWMKSLVFNSKGCTVFDSKGNLIYRVDNYDSKSWSNEVYFMDLNGKILFTLRQ 98

Query: 87  KKFSILGGWEGFRWIESERSKKASFGVKKLYRTASRKSNQSVCEINVGFERFSLVKMDGK 146
           KK      WEG+       S    F ++K+++   R+S+ S  ++ +G     +V  D +
Sbjct: 99  KKLGFFKSWEGY------NSTGTRFRLRKIFKILPRESSSSY-KVVMGSR---IVDGDQQ 158

Query: 147 LAFRIINI---------NGEVVAEAKRKVS-WNGILLGDDVLSLNANSQIERSLIMALVT 202
             ++I+N          +G ++AE K K+S  +G+ LGDDVL++    Q++ SLIM +V 
Sbjct: 159 SCYKIVNRGSVFAIKDGSGRLMAEVKNKLSDISGLDLGDDVLTMMVEPQLDHSLIMGIVI 214

[Close]

BLAST of Bhi12M000228 vs. TAIR 10
Match: AT1G53870.1 (Protein of unknown function (DUF567) )

HSP 1 Score: 132.9 bits (333), Expect = 2.9e-31
Identity = 76/186 (40.86%), Postives = 115/186 (61.83%), Query Frame = 0


Query: 27  TESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVDNYDRKC-SREVFLMDLRGKVLFTIRK 86
           TE E FTIWMKSL++++ GCTVFDS G+++YRVDNYD K  S EV+ MDL GK+LFT+R+
Sbjct: 39  TEQERFTIWMKSLVFNSKGCTVFDSKGNLIYRVDNYDSKSWSNEVYFMDLNGKILFTLRQ 98

Query: 87  KKFSILGGWEGFRWIESERSKKASFGVKKLYRTASRKSNQSVCEINVGFERFSLVKMDGK 146
           KK      WEG+       S    F ++K+++   R+S+ S  ++ +G     +V  D +
Sbjct: 99  KKLGFFKSWEGY------NSTGTRFRLRKIFKILPRESSSSY-KVVMGSR---IVDGDQQ 158

Query: 147 LAFRIINI---------NGEVVAEAKRKVS-WNGILLGDDVLSLNANSQIERSLIMALVT 202
             ++I+N          +G ++AE K K+S  +G+ LGDDVL++    Q++ SLIM +V 
Sbjct: 159 SCYKIVNRGSVFAIKDGSGRLMAEVKNKLSDISGLDLGDDVLTMMVEPQLDHSLIMGIVI 214

[Close]

BLAST of Bhi12M000228 vs. TAIR 10
Match: AT1G53870.2 (Protein of unknown function (DUF567) )

HSP 1 Score: 119.0 bits (297), Expect = 4.4e-27
Identity = 68/164 (41.46%), Postives = 103/164 (62.80%), Query Frame = 0


Query: 27  TESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVDNYDRKC-SREVFLMDLRGKVLFTIRK 86
           TE E FTIWMKSL++++ GCTVFDS G+++YRVDNYD K  S EV+ MDL GK+LFT+R+
Sbjct: 39  TEQERFTIWMKSLVFNSKGCTVFDSKGNLIYRVDNYDSKSWSNEVYFMDLNGKILFTLRQ 98

Query: 87  KKFSILGGWEGFRWIESERSKKASFGVKKLYRTASRKSNQSVCEINVGFERFSLVKMDGK 146
           KK      WEG+       S    F ++K+++   R+S+ S  ++ +G     +V  D +
Sbjct: 99  KKLGFFKSWEGY------NSTGTRFRLRKIFKILPRESSSSY-KVVMGSR---IVDGDQQ 158

Query: 147 LAFRIINI---------NGEVVAEAKRKVS-WNGILLGDDVLSL 180
             ++I+N          +G ++AE K K+S  +G+ LGDDVL++
Sbjct: 159 SCYKIVNRGSVFAIKDGSGRLMAEVKNKLSDISGLDLGDDVLTM 192

[Close]

BLAST of Bhi12M000228 vs. ExPASy Swiss-Prot
Match: Q9SH27 (Protein LURP-one-related 4 OS=Arabidopsis thaliana OX=3702 GN=At1g63410 PE=3 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 3.7e-39
Identity = 96/202 (47.52%), Postives = 132/202 (65.35%), Query Frame = 0


Query: 1   MAKVYPQTLPSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD 60
           MA+V+PQ     +  SSP  S     TE ETFT+WMKSL+Y TNG TV++SNG+I YRV+
Sbjct: 1   MARVFPQ-----AAISSPYMS-----TERETFTVWMKSLVYQTNGLTVYNSNGEITYRVE 60

Query: 61  NYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRTAS 120
           NYD KCS EV +MDL G +LFTIRKKK  + G W  +R   S  S +      ++ R++ 
Sbjct: 61  NYD-KCSNEVHIMDLHGNILFTIRKKKLWLFGSWYVYRECGSFTSTEEVKPCARIKRSSI 120

Query: 121 RKSNQSV-CEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLSL 180
           R  +  V  E N   E F +++ D K AF+II+I+G ++A+ K K S NGI LG+DVL+L
Sbjct: 121 RDGDWEVRDETN---EVFWILRFDPKFAFQIIDIHGNIIAQVKPKQSSNGITLGEDVLTL 180

Query: 181 NANSQIERSLIMALVTVYGLIR 202
               +++ SL++ LVTVYGLI+
Sbjct: 181 EVKPRVDHSLVVTLVTVYGLIK 188

[Close]

BLAST of Bhi12M000228 vs. ExPASy Swiss-Prot
Match: Q9LUM1 (Protein LURP-one-related 11 OS=Arabidopsis thaliana OX=3702 GN=At3g14260 PE=2 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 5.6e-35
Identity = 92/210 (43.81%), Postives = 126/210 (60.00%), Query Frame = 0


Query: 1   MAKVYPQTLPSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD 60
           M K++P    S    +   +SS    TE E+FTIWMKSL+++TNGCTVFDS G+I+YRVD
Sbjct: 23  MVKIHPDLTTS---GAGEETSSPYLTTEQESFTIWMKSLVFNTNGCTVFDSKGNIIYRVD 82

Query: 61  NYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFR----WIESERSKKASFGVKKLY 120
           NY+ K  REV+LMDL G VLFT+R++KF +   WEG+R     +ES  +K   F VK   
Sbjct: 83  NYNSKSCREVYLMDLSGHVLFTLRRQKFGLFKTWEGYRSSSATVES-TTKLEYFRVKNNV 142

Query: 121 RTASRKSNQSVCEINVGFER------FSLVKMDGKLAFRIININGEVVAEAKRKVSWNGI 180
                K + S   +N G  R      + +V     LA  I +  G+++AE KRK S NG+
Sbjct: 143 FQVPNKDSSSSYRVNAGSCRNDEQYCYKMVTRGSSLA--IEDNCGKLLAEVKRKQSINGL 202

Query: 181 LLGDDVLSLNANSQIERSLIMALVTVYGLI 201
            LGDDVL++   SQ++ S I+ LV  + LI
Sbjct: 203 KLGDDVLTMMVESQVDHSFIIGLVLAHSLI 226

[Close]

BLAST of Bhi12M000228 vs. ExPASy Swiss-Prot
Match: Q8LG32 (Protein LURP-one-related 2 OS=Arabidopsis thaliana OX=3702 GN=At1g53870 PE=2 SV=2)

HSP 1 Score: 132.9 bits (333), Expect = 4.2e-30
Identity = 76/186 (40.86%), Postives = 115/186 (61.83%), Query Frame = 0


Query: 27  TESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVDNYDRKC-SREVFLMDLRGKVLFTIRK 86
           TE E FTIWMKSL++++ GCTVFDS G+++YRVDNYD K  S EV+ MDL GK+LFT+R+
Sbjct: 39  TEQERFTIWMKSLVFNSKGCTVFDSKGNLIYRVDNYDSKSWSNEVYFMDLNGKILFTLRQ 98

Query: 87  KKFSILGGWEGFRWIESERSKKASFGVKKLYRTASRKSNQSVCEINVGFERFSLVKMDGK 146
           KK      WEG+       S    F ++K+++   R+S+ S  ++ +G     +V  D +
Sbjct: 99  KKLGFFKSWEGY------NSTGTRFRLRKIFKILPRESSSSY-KVVMGSR---IVDGDQQ 158

Query: 147 LAFRIINI---------NGEVVAEAKRKVS-WNGILLGDDVLSLNANSQIERSLIMALVT 202
             ++I+N          +G ++AE K K+S  +G+ LGDDVL++    Q++ SLIM +V 
Sbjct: 159 SCYKIVNRGSVFAIKDGSGRLMAEVKNKLSDISGLDLGDDVLTMMVEPQLDHSLIMGIVI 214

[Close]

BLAST of Bhi12M000228 vs. ExPASy Swiss-Prot
Match: Q67XV7 (Protein LURP-one-related 3 OS=Arabidopsis thaliana OX=3702 GN=At1g53890 PE=2 SV=2)

HSP 1 Score: 132.9 bits (333), Expect = 4.2e-30
Identity = 76/186 (40.86%), Postives = 115/186 (61.83%), Query Frame = 0


Query: 27  TESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVDNYDRKC-SREVFLMDLRGKVLFTIRK 86
           TE E FTIWMKSL++++ GCTVFDS G+++YRVDNYD K  S EV+ MDL GK+LFT+R+
Sbjct: 39  TEQERFTIWMKSLVFNSKGCTVFDSKGNLIYRVDNYDSKSWSNEVYFMDLNGKILFTLRQ 98

Query: 87  KKFSILGGWEGFRWIESERSKKASFGVKKLYRTASRKSNQSVCEINVGFERFSLVKMDGK 146
           KK      WEG+       S    F ++K+++   R+S+ S  ++ +G     +V  D +
Sbjct: 99  KKLGFFKSWEGY------NSTGTRFRLRKIFKILPRESSSSY-KVVMGSR---IVDGDQQ 158

Query: 147 LAFRIINI---------NGEVVAEAKRKVS-WNGILLGDDVLSLNANSQIERSLIMALVT 202
             ++I+N          +G ++AE K K+S  +G+ LGDDVL++    Q++ SLIM +V 
Sbjct: 159 SCYKIVNRGSVFAIKDGSGRLMAEVKNKLSDISGLDLGDDVLTMMVEPQLDHSLIMGIVI 214

[Close]

BLAST of Bhi12M000228 vs. ExPASy Swiss-Prot
Match: Q9ZVI6 (Protein LURP-one-related 8 OS=Arabidopsis thaliana OX=3702 GN=At2g38640 PE=2 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 1.2e-13
Identity = 62/203 (30.54%), Postives = 100/203 (49.26%), Query Frame = 0


Query: 1   MAKVYPQTLPSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD 60
           M KV+P+         S    SLC    +   T+W KSL+++ +G TV+++NG++V+RVD
Sbjct: 1   MTKVHPK-------FPSTCEESLCDSKAAVVLTVWKKSLLFNCDGFTVYNANGELVFRVD 60

Query: 61  NYDRKCSRE-VFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRTA 120
           NY   C R+ + LMD  G  L +IR+KK S+   W  +   + E  +   F  +K     
Sbjct: 61  NY-MNCPRDNIVLMDASGFPLLSIRRKKLSLGDCWMVY---DGETERDPIFTARKNVSII 120

Query: 121 SRKSNQSVCEINVGFERFSLVKMDGKLAFRIINI-----NGEVVAEAKRKVS-WNGILLG 180
           S + + +     V  ++  L +++G    R   I     N +  AE KRK +   G+  G
Sbjct: 121 SNRKSLAW----VSAKKTVLYEIEGSYGQRSCKILDERRNKKKTAEIKRKETVIGGVAFG 180

Query: 181 DDVLSLNANSQIERSLIMALVTV 197
            DV  L   S++E  + MAL  +
Sbjct: 181 KDVYKLIVESEMEPRVAMALTII 188

[Close]

BLAST of Bhi12M000228 vs. NCBI nr
Match: XP_038875222.1 (protein LURP-one-related 4 isoform X2 [Benincasa hispida])

HSP 1 Score: 399.8 bits (1026), Expect = 1.4e-107
Identity = 204/204 (100.00%), Postives = 204/204 (100.00%), Query Frame = 0


Query: 1   MAKVYPQTLPSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD 60
           MAKVYPQTLPSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD
Sbjct: 1   MAKVYPQTLPSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD 60

Query: 61  NYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRTAS 120
           NYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRTAS
Sbjct: 61  NYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRTAS 120

Query: 121 RKSNQSVCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLSLN 180
           RKSNQSVCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLSLN
Sbjct: 121 RKSNQSVCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLSLN 180

Query: 181 ANSQIERSLIMALVTVYGLIRRQM 205
           ANSQIERSLIMALVTVYGLIRRQM
Sbjct: 181 ANSQIERSLIMALVTVYGLIRRQM 204

[Close]

BLAST of Bhi12M000228 vs. NCBI nr
Match: XP_038875221.1 (protein LURP-one-related 4 isoform X1 [Benincasa hispida])

HSP 1 Score: 384.0 bits (985), Expect = 7.9e-103
Identity = 204/234 (87.18%), Postives = 204/234 (87.18%), Query Frame = 0


Query: 1   MAKVYPQTLPSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD 60
           MAKVYPQTLPSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD
Sbjct: 1   MAKVYPQTLPSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD 60

Query: 61  NYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRTAS 120
           NYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRTAS
Sbjct: 61  NYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRTAS 120

Query: 121 RKSNQSVCEINVGFERFSLVKMDGKLAFRIININGEVVAE-------------------- 180
           RKSNQSVCEINVGFERFSLVKMDGKLAFRIININGEVVAE                    
Sbjct: 121 RKSNQSVCEINVGFERFSLVKMDGKLAFRIININGEVVAEVIINLNLNLNLNLNLVEADL 180

Query: 181 ----------AKRKVSWNGILLGDDVLSLNANSQIERSLIMALVTVYGLIRRQM 205
                     AKRKVSWNGILLGDDVLSLNANSQIERSLIMALVTVYGLIRRQM
Sbjct: 181 KIIDLIVLLQAKRKVSWNGILLGDDVLSLNANSQIERSLIMALVTVYGLIRRQM 234

[Close]

BLAST of Bhi12M000228 vs. NCBI nr
Match: XP_004143921.1 (protein LURP-one-related 4 [Cucumis sativus] >KGN50117.1 hypothetical protein Csa_000326 [Cucumis sativus])

HSP 1 Score: 369.4 bits (947), Expect = 2.0e-98
Identity = 191/207 (92.27%), Postives = 197/207 (95.17%), Query Frame = 0


Query: 1   MAKVYPQTL--PSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYR 60
           MAKVYPQT   PSPS SSSPSSSSLCSETE+ETFTIWMKSLIYHTNGCT+FDSNGDIVYR
Sbjct: 1   MAKVYPQTALSPSPSSSSSPSSSSLCSETETETFTIWMKSLIYHTNGCTIFDSNGDIVYR 60

Query: 61  VDNYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRT 120
           VDNYDRKCS EVFLMDLRGKVLFTIRKKKFSILGGWEG+RW+ESERSKK SFGVKKLYR+
Sbjct: 61  VDNYDRKCSSEVFLMDLRGKVLFTIRKKKFSILGGWEGYRWMESERSKKPSFGVKKLYRS 120

Query: 121 ASRKSNQS-VCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVL 180
            SRK NQS VCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVL
Sbjct: 121 GSRKRNQSAVCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVL 180

Query: 181 SLNANSQIERSLIMALVTVYGLIRRQM 205
           SLNANSQIE SL+MALVTVYGLIRRQM
Sbjct: 181 SLNANSQIETSLVMALVTVYGLIRRQM 207

[Close]

BLAST of Bhi12M000228 vs. NCBI nr
Match: XP_008437245.1 (PREDICTED: protein LURP-one-related 4 [Cucumis melo])

HSP 1 Score: 355.5 bits (911), Expect = 3.0e-94
Identity = 187/205 (91.22%), Postives = 192/205 (93.66%), Query Frame = 0


Query: 1   MAKVYPQTLPSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD 60
           MAKVYPQT  SPS SSS SSSSL SET  ETFTIWMKSLIYHTNGCT+FDSNGDIVYRVD
Sbjct: 1   MAKVYPQTALSPSTSSSSSSSSLGSET--ETFTIWMKSLIYHTNGCTIFDSNGDIVYRVD 60

Query: 61  NYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRTAS 120
           NYDRKCS EVFLMDLRGKVLFTIRKKKFSILGGWEG+RW+ESERSKK SFGVKKLYR+ S
Sbjct: 61  NYDRKCSSEVFLMDLRGKVLFTIRKKKFSILGGWEGYRWMESERSKKPSFGVKKLYRSGS 120

Query: 121 RKSNQS-VCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLSL 180
           RK NQS VCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLSL
Sbjct: 121 RKRNQSAVCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLSL 180

Query: 181 NANSQIERSLIMALVTVYGLIRRQM 205
           NANSQIE SL+MALVTVYGLIRRQM
Sbjct: 181 NANSQIETSLVMALVTVYGLIRRQM 203

[Close]

BLAST of Bhi12M000228 vs. NCBI nr
Match: XP_023551055.1 (protein LURP-one-related 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 353.6 bits (906), Expect = 1.1e-93
Identity = 183/204 (89.71%), Postives = 190/204 (93.14%), Query Frame = 0


Query: 1   MAKVYPQTLPSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD 60
           MAKVYPQ+ PSPSPSSSPSSS LCSET  ETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD
Sbjct: 1   MAKVYPQSAPSPSPSSSPSSSPLCSET--ETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD 60

Query: 61  NYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRTAS 120
           NYDRKCSREVFLMDLRGKVLFTIRKKKFS L GWEG+RWIESERS   SF V+KLYR+ S
Sbjct: 61  NYDRKCSREVFLMDLRGKVLFTIRKKKFSFLRGWEGYRWIESERSMNPSFRVRKLYRSGS 120

Query: 121 RKSNQSVCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLSLN 180
           RK N+S+CEI+VGFERFSLVKMDGKLAFRIININGEVVAEAKRKVS NGILLGDDVLSLN
Sbjct: 121 RKRNESICEISVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSSNGILLGDDVLSLN 180

Query: 181 ANSQIERSLIMALVTVYGLIRRQM 205
           A SQIERSLIMALVTVYGLIRRQM
Sbjct: 181 ATSQIERSLIMALVTVYGLIRRQM 202

[Close]

BLAST of Bhi12M000228 vs. ExPASy TrEMBL
Match: A0A0A0KNY3 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G154790 PE=3 SV=1)

HSP 1 Score: 369.4 bits (947), Expect = 9.7e-99
Identity = 191/207 (92.27%), Postives = 197/207 (95.17%), Query Frame = 0


Query: 1   MAKVYPQTL--PSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYR 60
           MAKVYPQT   PSPS SSSPSSSSLCSETE+ETFTIWMKSLIYHTNGCT+FDSNGDIVYR
Sbjct: 1   MAKVYPQTALSPSPSSSSSPSSSSLCSETETETFTIWMKSLIYHTNGCTIFDSNGDIVYR 60

Query: 61  VDNYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRT 120
           VDNYDRKCS EVFLMDLRGKVLFTIRKKKFSILGGWEG+RW+ESERSKK SFGVKKLYR+
Sbjct: 61  VDNYDRKCSSEVFLMDLRGKVLFTIRKKKFSILGGWEGYRWMESERSKKPSFGVKKLYRS 120

Query: 121 ASRKSNQS-VCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVL 180
            SRK NQS VCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVL
Sbjct: 121 GSRKRNQSAVCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVL 180

Query: 181 SLNANSQIERSLIMALVTVYGLIRRQM 205
           SLNANSQIE SL+MALVTVYGLIRRQM
Sbjct: 181 SLNANSQIETSLVMALVTVYGLIRRQM 207

[Close]

BLAST of Bhi12M000228 vs. ExPASy TrEMBL
Match: A0A1S3AT73 (protein LURP-one-related 4 OS=Cucumis melo OX=3656 GN=LOC103482731 PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.5e-94
Identity = 187/205 (91.22%), Postives = 192/205 (93.66%), Query Frame = 0


Query: 1   MAKVYPQTLPSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD 60
           MAKVYPQT  SPS SSS SSSSL SET  ETFTIWMKSLIYHTNGCT+FDSNGDIVYRVD
Sbjct: 1   MAKVYPQTALSPSTSSSSSSSSLGSET--ETFTIWMKSLIYHTNGCTIFDSNGDIVYRVD 60

Query: 61  NYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRTAS 120
           NYDRKCS EVFLMDLRGKVLFTIRKKKFSILGGWEG+RW+ESERSKK SFGVKKLYR+ S
Sbjct: 61  NYDRKCSSEVFLMDLRGKVLFTIRKKKFSILGGWEGYRWMESERSKKPSFGVKKLYRSGS 120

Query: 121 RKSNQS-VCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLSL 180
           RK NQS VCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLSL
Sbjct: 121 RKRNQSAVCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLSL 180

Query: 181 NANSQIERSLIMALVTVYGLIRRQM 205
           NANSQIE SL+MALVTVYGLIRRQM
Sbjct: 181 NANSQIETSLVMALVTVYGLIRRQM 203

[Close]

BLAST of Bhi12M000228 vs. ExPASy TrEMBL
Match: A0A5A7TMZ0 (Protein LURP-one-related 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002750 PE=3 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 6.1e-93
Identity = 186/205 (90.73%), Postives = 191/205 (93.17%), Query Frame = 0


Query: 1   MAKVYPQTLPSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD 60
           MAKVYPQT  SPS SS  SSSSL SET  ETFTIWMKSLIYHTNGCT+FDSNGDIVYRVD
Sbjct: 1   MAKVYPQTALSPSTSS--SSSSLGSET--ETFTIWMKSLIYHTNGCTIFDSNGDIVYRVD 60

Query: 61  NYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRTAS 120
           NYDRKCS EVFLMDLRGKVLFTIRKKKFSILGGWEG+RW+ESERSKK SFGVKKLYR+ S
Sbjct: 61  NYDRKCSSEVFLMDLRGKVLFTIRKKKFSILGGWEGYRWMESERSKKPSFGVKKLYRSGS 120

Query: 121 RKSNQS-VCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLSL 180
           RK NQS VCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLSL
Sbjct: 121 RKRNQSAVCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLSL 180

Query: 181 NANSQIERSLIMALVTVYGLIRRQM 205
           NANSQIE SL+MALVTVYGLIRRQM
Sbjct: 181 NANSQIETSLVMALVTVYGLIRRQM 201

[Close]

BLAST of Bhi12M000228 vs. ExPASy TrEMBL
Match: A0A6J1ELI2 (protein LURP-one-related 4-like OS=Cucurbita moschata OX=3662 GN=LOC111435723 PE=3 SV=1)

HSP 1 Score: 348.6 bits (893), Expect = 1.8e-92
Identity = 183/206 (88.83%), Postives = 190/206 (92.23%), Query Frame = 0


Query: 1   MAKVYPQTL--PSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYR 60
           MAKVYPQ+   PSPSPSSSPSSS LCSET  ETFTIWMKSLIYHTNGCTVFDSNGDIVYR
Sbjct: 1   MAKVYPQSAPSPSPSPSSSPSSSPLCSET--ETFTIWMKSLIYHTNGCTVFDSNGDIVYR 60

Query: 61  VDNYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRT 120
           VDNYDRKCSREVFLMDLRGKVLFTIRKKKFS L GWEG+RWIESERS   SF V+KLYR+
Sbjct: 61  VDNYDRKCSREVFLMDLRGKVLFTIRKKKFSFLRGWEGYRWIESERSMNPSFRVRKLYRS 120

Query: 121 ASRKSNQSVCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLS 180
            SRK N+S+CEI+VGFERFSLVKMDGKLAFRIININGEVVAEAKRKVS NGILLGDDVLS
Sbjct: 121 GSRKRNESICEISVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSSNGILLGDDVLS 180

Query: 181 LNANSQIERSLIMALVTVYGLIRRQM 205
           LNA SQIERSLIMALVTVYGLIRRQM
Sbjct: 181 LNATSQIERSLIMALVTVYGLIRRQM 204

[Close]

BLAST of Bhi12M000228 vs. ExPASy TrEMBL
Match: A0A6J1HYX0 (protein LURP-one-related 4-like OS=Cucurbita maxima OX=3661 GN=LOC111468925 PE=3 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 5.7e-91
Identity = 180/204 (88.24%), Postives = 187/204 (91.67%), Query Frame = 0


Query: 1   MAKVYPQTLPSPSPSSSPSSSSLCSETESETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD 60
           MAKVYPQ+ PSPSP  SPSSS LCSET  ETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD
Sbjct: 1   MAKVYPQSAPSPSP--SPSSSPLCSET--ETFTIWMKSLIYHTNGCTVFDSNGDIVYRVD 60

Query: 61  NYDRKCSREVFLMDLRGKVLFTIRKKKFSILGGWEGFRWIESERSKKASFGVKKLYRTAS 120
           NYDRKCSREVFLMDLRGKVLFTIRKKKFS L GWEG+RWIESERS   SF V+KLYR+ S
Sbjct: 61  NYDRKCSREVFLMDLRGKVLFTIRKKKFSFLRGWEGYRWIESERSMNPSFRVRKLYRSRS 120

Query: 121 RKSNQSVCEINVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSWNGILLGDDVLSLN 180
           R  N+S+CEI+VGFERFSLVKMDGKLAFRIININGEVVAEAKRKVS NGILLGDDVLSLN
Sbjct: 121 RMRNESICEISVGFERFSLVKMDGKLAFRIININGEVVAEAKRKVSSNGILLGDDVLSLN 180

Query: 181 ANSQIERSLIMALVTVYGLIRRQM 205
           A SQIERSLIMALVTVYGLIRRQM
Sbjct: 181 ATSQIERSLIMALVTVYGLIRRQM 200

The following BLAST results are available for this feature:

BLAST of Bhi12M000228 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G63410.1	3.3e-38	45.32	Protein of unknown function (DUF567)	[more]
AT3G14260.1	4.0e-36	43.81	Protein of unknown function (DUF567)	[more]
AT1G53890.1	2.9e-31	40.86	Protein of unknown function (DUF567)	[more]
AT1G53870.1	2.9e-31	40.86	Protein of unknown function (DUF567)	[more]
AT1G53870.2	4.4e-27	41.46	Protein of unknown function (DUF567)	[more]

BLAST of Bhi12M000228 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SH27	3.7e-39	47.52	Protein LURP-one-related 4 OS=Arabidopsis thaliana OX=3702 GN=At1g63410 PE=3 SV=...	[more]
Q9LUM1	5.6e-35	43.81	Protein LURP-one-related 11 OS=Arabidopsis thaliana OX=3702 GN=At3g14260 PE=2 SV...	[more]
Q8LG32	4.2e-30	40.86	Protein LURP-one-related 2 OS=Arabidopsis thaliana OX=3702 GN=At1g53870 PE=2 SV=...	[more]
Q67XV7	4.2e-30	40.86	Protein LURP-one-related 3 OS=Arabidopsis thaliana OX=3702 GN=At1g53890 PE=2 SV=...	[more]
Q9ZVI6	1.2e-13	30.54	Protein LURP-one-related 8 OS=Arabidopsis thaliana OX=3702 GN=At2g38640 PE=2 SV=...	[more]

BLAST of Bhi12M000228 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875222.1	1.4e-107	100.00	protein LURP-one-related 4 isoform X2 [Benincasa hispida]	[more]
XP_038875221.1	7.9e-103	87.18	protein LURP-one-related 4 isoform X1 [Benincasa hispida]	[more]
XP_004143921.1	2.0e-98	92.27	protein LURP-one-related 4 [Cucumis sativus] >KGN50117.1 hypothetical protein Cs...	[more]
XP_008437245.1	3.0e-94	91.22	PREDICTED: protein LURP-one-related 4 [Cucumis melo]	[more]
XP_023551055.1	1.1e-93	89.71	protein LURP-one-related 4-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Bhi12M000228 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KNY3	9.7e-99	92.27	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G154790 PE=3 SV=1	[more]
A0A1S3AT73	1.5e-94	91.22	protein LURP-one-related 4 OS=Cucumis melo OX=3656 GN=LOC103482731 PE=3 SV=1	[more]
A0A5A7TMZ0	6.1e-93	90.73	Protein LURP-one-related 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff...	[more]
A0A6J1ELI2	1.8e-92	88.83	protein LURP-one-related 4-like OS=Cucurbita moschata OX=3662 GN=LOC111435723 PE...	[more]
A0A6J1HYX0	5.7e-91	88.24	protein LURP-one-related 4-like OS=Cucurbita maxima OX=3661 GN=LOC111468925 PE=3...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR038595	LURP-one-related superfamily	GENE3D	2.40.160.200		coord: 20..204 e-value: 1.2E-37 score: 131.2
IPR007612	LURP-one-related	PFAM	PF04525	LOR	coord: 21..196 e-value: 5.6E-43 score: 146.7
IPR007612	LURP-one-related	PANTHER	PTHR31087	FAMILY NOT NAMED	coord: 2..203
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 8..24
None	No IPR available	PANTHER	PTHR31087:SF59	PROTEIN LURP-ONE-RELATED 4	coord: 2..203
IPR025659	Tubby-like, C-terminal	SUPERFAMILY	54518	Tubby C-terminal domain-like	coord: 26..200

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Bhi12G000228	Bhi12G000228	gene

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi12M000228.exon3	Bhi12M000228.exon3	exon
Bhi12M000228.exon2	Bhi12M000228.exon2	exon
Bhi12M000228.exon1	Bhi12M000228.exon1	exon

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi12M000228.utr3p1	Bhi12M000228.utr3p1	three_prime_UTR

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Bhi12M000228	cds.Bhi12M000228_3	CDS
cds.Bhi12M000228	cds.Bhi12M000228_2	CDS
cds.Bhi12M000228	cds.Bhi12M000228	CDS

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi12M000228.utr5p1	Bhi12M000228.utr5p1	five_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Bhi12M000228	Bhi12M000228-protein	polypeptide