Bhi10M001012 (mRNA) Wax gourd (B227) v1

Overview


Name	Bhi10M001012
Type	mRNA
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Nitrile-specifier protein 5
Location	chr10: 31087488 .. 31089038 (+)
Sequence length	1469
RNA-Seq Expression	Bhi10M001012
Synteny	Bhi10M001012

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTAAATCTAGTACCTATAGTCGAATAAAACCTCATAAATTGAAATATCATGCCTATTTGAACTTTAACAACTAATATATATATATATATTAGTTATCAAAGAATGCCCTGCCAGACCCTCACCATTGCATTAGAAGATTGAATTCTCTGGACTCTCAACAAATGGCTCCCACGAATGGAAAATGGACTAAGGTCAGTATCTTCATCTAATCATAACTATCTATTCAATTTTCAATTCCATTTTTTCACAAAATTTCCCAGAAATTCTTTGCAGATCGATCAAAAGACCGCAGGCCCAGGCGCAAGAAGCTCTCACGCCATCACAATCGTAGCCCAAAAGGCCTACGCCTTCGGCGGCGAATTCTCCCCCCGAGTCCCCGTCGACAACAAACTCCACGTCTTCGACCTCCACGAACACATCTGGACGGTCGCCGTCGTCTCCGGCGATGTCCCTCCGCCACGCGTTGGCGTCACGATGGCCTCTGTCGGCCGAATCATTTACGTCTTCGGCGGTAGGGATTCAGAGCACACGGAGCTAAACGAGCTCTACTCCTTCGACACATCCAACAGCAAATGGACTCTCCTCTCCGGCAGCGACTCCGGACCACCACATCGGAGCTACCACTCCACCACGGCCGACGACCGCCGCGTATACATCTTCGGTGGGTGCGGGAACTCCGGCCGCCTCAACGACTTGTGGGCTTACGATACAGTAGATGGAAAATGGATGGAGTATCCGGCAGGAGGGGAAAGGTTAAAGGGTCGAGGTGGGCCGGGCCTGGCTGTGGCCCAAGATAAAATATGGGTTGTGTACGGATTCGCGGGTATGGAAATGGACGACGTTCACTATTTCGATGTGGCCCAAAACCAATGGGCCCAAGTTGAGACTAGCGGGGAAAAGCCGACGGCTAGGAGCGTGTTCTGCACGTGCGCGATTGGGAAGTACGTGTATATATACGGTGGGGAAGTGGACCCCAGTGATCTGGGGCACTTGGGGGCCGGGAAGTTCGCGGGAGAGGTTTACGTGTTGGACACGGAGGCGTTGGAGTGGAGTAGAGTGGAAGATGGGCTGGGCATGGAGAATCACCCTGGGCCGAGGGGTTGGTGTGCGTTTTCTGTGGGCCGGAATGGAGAGGAATTGGGCCTTCTAGTTTATGGGGGTAATTCCCCGAGCAATGATCGACTGGGGGATATCTACTTTTTTGCTCCGTGTGAGTGAGAAAATGGTGAATCATGTTGGTGATTTGATGATAGGTGATGGGATTTTCTTTTTTATGAGTTATATTGGCCTTTTTGGAATAAAAGGCTAAAATGTTAATATAGATGGAGGGAGAGGGTGTGATGAGAGAGGAGTTTAATGAGTGATAATGTTGGCGAATATAAAAATAAGAGGAGTGATTATATATTGTGTATTTTGCATAATTATAATGCAGTAAATTGGTGGTAATTTACCTTTTTAATTTGATTTTAGTTTTAAATTCTTAAAATGATTGGTTCAACTAGGAACAATAATTTTTTTGGGCTGTCCTAAAAAAAACAATCATTTTTTTG

mRNA sequence

TTTAAATCTAGTACCTATAGTCGAATAAAACCTCATAAATTGAAATATCATGCCTATTTGAACTTTAACAACTAATATATATATATATATTAGTTATCAAAGAATGCCCTGCCAGACCCTCACCATTGCATTAGAAGATTGAATTCTCTGGACTCTCAACAAATGGCTCCCACGAATGGAAAATGGACTAAGATCGATCAAAAGACCGCAGGCCCAGGCGCAAGAAGCTCTCACGCCATCACAATCGTAGCCCAAAAGGCCTACGCCTTCGGCGGCGAATTCTCCCCCCGAGTCCCCGTCGACAACAAACTCCACGTCTTCGACCTCCACGAACACATCTGGACGGTCGCCGTCGTCTCCGGCGATGTCCCTCCGCCACGCGTTGGCGTCACGATGGCCTCTGTCGGCCGAATCATTTACGTCTTCGGCGGTAGGGATTCAGAGCACACGGAGCTAAACGAGCTCTACTCCTTCGACACATCCAACAGCAAATGGACTCTCCTCTCCGGCAGCGACTCCGGACCACCACATCGGAGCTACCACTCCACCACGGCCGACGACCGCCGCGTATACATCTTCGGTGGGTGCGGGAACTCCGGCCGCCTCAACGACTTGTGGGCTTACGATACAGTAGATGGAAAATGGATGGAGTATCCGGCAGGAGGGGAAAGGTTAAAGGGTCGAGGTGGGCCGGGCCTGGCTGTGGCCCAAGATAAAATATGGGTTGTGTACGGATTCGCGGGTATGGAAATGGACGACGTTCACTATTTCGATGTGGCCCAAAACCAATGGGCCCAAGTTGAGACTAGCGGGGAAAAGCCGACGGCTAGGAGCGTGTTCTGCACGTGCGCGATTGGGAAGTACGTGTATATATACGGTGGGGAAGTGGACCCCAGTGATCTGGGGCACTTGGGGGCCGGGAAGTTCGCGGGAGAGGTTTACGTGTTGGACACGGAGGCGTTGGAGTGGAGTAGAGTGGAAGATGGGCTGGGCATGGAGAATCACCCTGGGCCGAGGGGTTGGTGTGCGTTTTCTGTGGGCCGGAATGGAGAGGAATTGGGCCTTCTAGTTTATGGGGGTAATTCCCCGAGCAATGATCGACTGGGGGATATCTACTTTTTTGCTCCGTGTGAGTGAGAAAATGGTGAATCATGTTGGTGATTTGATGATAGGTGATGGGATTTTCTTTTTTATGAGTTATATTGGCCTTTTTGGAATAAAAGGCTAAAATGTTAATATAGATGGAGGGAGAGGGTGTGATGAGAGAGGAGTTTAATGAGTGATAATGTTGGCGAATATAAAAATAAGAGGAGTGATTATATATTGTGTATTTTGCATAATTATAATGCAGTAAATTGGTGGTAATTTACCTTTTTAATTTGATTTTAGTTTTAAATTCTTAAAATGATTGGTTCAACTAGGAACAATAATTTTTTTGGGCTGTCCTAAAAAAAACAATCATTTTTTTG

Coding sequence (CDS)

ATGGCTCCCACGAATGGAAAATGGACTAAGATCGATCAAAAGACCGCAGGCCCAGGCGCAAGAAGCTCTCACGCCATCACAATCGTAGCCCAAAAGGCCTACGCCTTCGGCGGCGAATTCTCCCCCCGAGTCCCCGTCGACAACAAACTCCACGTCTTCGACCTCCACGAACACATCTGGACGGTCGCCGTCGTCTCCGGCGATGTCCCTCCGCCACGCGTTGGCGTCACGATGGCCTCTGTCGGCCGAATCATTTACGTCTTCGGCGGTAGGGATTCAGAGCACACGGAGCTAAACGAGCTCTACTCCTTCGACACATCCAACAGCAAATGGACTCTCCTCTCCGGCAGCGACTCCGGACCACCACATCGGAGCTACCACTCCACCACGGCCGACGACCGCCGCGTATACATCTTCGGTGGGTGCGGGAACTCCGGCCGCCTCAACGACTTGTGGGCTTACGATACAGTAGATGGAAAATGGATGGAGTATCCGGCAGGAGGGGAAAGGTTAAAGGGTCGAGGTGGGCCGGGCCTGGCTGTGGCCCAAGATAAAATATGGGTTGTGTACGGATTCGCGGGTATGGAAATGGACGACGTTCACTATTTCGATGTGGCCCAAAACCAATGGGCCCAAGTTGAGACTAGCGGGGAAAAGCCGACGGCTAGGAGCGTGTTCTGCACGTGCGCGATTGGGAAGTACGTGTATATATACGGTGGGGAAGTGGACCCCAGTGATCTGGGGCACTTGGGGGCCGGGAAGTTCGCGGGAGAGGTTTACGTGTTGGACACGGAGGCGTTGGAGTGGAGTAGAGTGGAAGATGGGCTGGGCATGGAGAATCACCCTGGGCCGAGGGGTTGGTGTGCGTTTTCTGTGGGCCGGAATGGAGAGGAATTGGGCCTTCTAGTTTATGGGGGTAATTCCCCGAGCAATGATCGACTGGGGGATATCTACTTTTTTGCTCCGTGTGAGTGA

Protein sequence

MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIWTVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSGPPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLAVAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGGEVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELGLLVYGGNSPSNDRLGDIYFFAPCE

Homology

[Close]

BLAST of Bhi10M001012 vs. TAIR 10
Match: AT3G07720.1 (Galactose oxidase/kelch repeat superfamily protein )

HSP 1 Score: 469.5 bits (1207), Expect = 2.1e-132
Identity = 209/320 (65.31%), Postives = 246/320 (76.88%), Query Frame = 0


Query: 3   PTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIWTV 62
           P  GKW ++ QK  GPGARSSHAI +V  K YAFGGEF PRVPVDN+L+VFDL    W++
Sbjct: 5   PMEGKWVQLKQKGTGPGARSSHAIALVGNKMYAFGGEFQPRVPVDNQLYVFDLETQTWSI 64

Query: 63  AVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSGPP 122
              SGD PPPRVGV MA+VG IIY FGGRDS H ELNELY F+T  ++W LLS  ++GP 
Sbjct: 65  QEASGDAPPPRVGVAMAAVGPIIYFFGGRDSTHQELNELYCFNTLTNQWKLLSSGETGPQ 124

Query: 123 HRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLAVA 182
           +RSYHS TAD + VY+FGGCG  GRLNDLWAY+ VD KW+++P+ GE  +GRGGPGL V 
Sbjct: 125 NRSYHSITADSQNVYVFGGCGVDGRLNDLWAYNVVDQKWIKFPSPGEACRGRGGPGLEVV 184

Query: 183 QDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGGEV 242
           Q KIWVVYGFAG E DDVH FD+A+ +W +VET GEKP+ARSVF T  +GK + I GGE+
Sbjct: 185 QGKIWVVYGFAGEEADDVHCFDIAKGEWKEVETKGEKPSARSVFSTAVVGKQILISGGEI 244

Query: 243 DPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELGLL 302
           DPSDLGH+GAG F G+ Y LDTE LEW + EDG+G   HPGPRGWCAF+ G    + GLL
Sbjct: 245 DPSDLGHMGAGCFTGDAYGLDTETLEWRKWEDGVGSVEHPGPRGWCAFAAGSRDGKQGLL 304

Query: 303 VYGGNSPSNDRLGDIYFFAP 323
           VYGGNSPSNDRL DI+FF P
Sbjct: 305 VYGGNSPSNDRLDDIFFFTP 324

[Close]

BLAST of Bhi10M001012 vs. TAIR 10
Match: AT5G48180.1 (nitrile specifier protein 5 )

HSP 1 Score: 372.1 bits (954), Expect = 4.6e-103
Identity = 171/320 (53.44%), Postives = 218/320 (68.12%), Query Frame = 0


Query: 1   MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIW 60
           M P   KW K+ QK AGPGARSSHA+T+V  K Y FGGE  P + +DN L+VFDL    W
Sbjct: 1   MCPVENKWLKVGQKGAGPGARSSHAMTVVGNKVYCFGGELKPTIHIDNDLYVFDLETQEW 60

Query: 61  TVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSG 120
           ++A  +G+ P P  GV+M ++G  IYV+GGRD +    N L+S+DT  ++W LL+  + G
Sbjct: 61  SIAPATGEAPFPCFGVSMVTIGSTIYVYGGRDDKR-RYNGLHSYDTETNEWKLLAPVEEG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLA 180
            P RSYHS   DDR+VY+FGG    GR+N L AYD VD KW+EYPA GE  KGRG PGL 
Sbjct: 121 LPGRSYHSMAGDDRKVYVFGGVTAKGRVNTLHAYDVVDQKWVEYPAAGEACKGRGAPGLV 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           V + +IWV++GF G E+ D+H FD+A  QW  VET+G+ P ARSVF   + GKY+ IYGG
Sbjct: 181 VVEGRIWVLFGFDGNELGDIHCFDLASEQWKAVETTGDVPAARSVFPAVSYGKYIVIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELG 300
           E +P +L H+GAGK +GEVY LDTE L W R+  G   E  P  RGWCAF+      E G
Sbjct: 241 EEEPHELMHMGAGKMSGEVYQLDTETLVWERIVCG-NEEEKPSQRGWCAFTKAVKDGEEG 300

Query: 301 LLVYGGNSPSNDRLGDIYFF 321
           LLV+GGNSP+N+RL D+ F+
Sbjct: 301 LLVHGGNSPTNERLDDLVFW 318

[Close]

BLAST of Bhi10M001012 vs. TAIR 10
Match: AT3G16410.1 (nitrile specifier protein 4 )

HSP 1 Score: 329.7 bits (844), Expect = 2.6e-90
Identity = 149/324 (45.99%), Postives = 210/324 (64.81%), Query Frame = 0


Query: 1   MAPT---NGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHE 60
           ++PT   +GKWTK+D+   GPG R SH I  V  K Y+FGGEF+P  P+D  L+VFD+  
Sbjct: 292 LSPTPNLHGKWTKVDENGDGPGLRCSHDIAQVGNKIYSFGGEFTPNQPIDKHLYVFDIES 351

Query: 61  HIWTVAVVSGDVPP-PRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSG 120
             W+++  +GD+P    +GV M S+G  +YVFGGRD+   + N  YSFDT+ ++W LL+ 
Sbjct: 352 RTWSISPATGDIPTLSCLGVCMVSIGSTLYVFGGRDASR-QYNGFYSFDTTTNEWKLLTP 411

Query: 121 SDSGPPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGG 180
            + GP  RS+HS  AD+  VY+FGG   + RLN L +Y+ VD KW      G+ L  RGG
Sbjct: 412 VEEGPTPRSFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGG 471

Query: 181 PGLAVAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVY 240
            GL V Q K+WVVYGF G E+DDVHY+D  Q++W QVET G +P+ RSVF + A+GK++ 
Sbjct: 472 AGLEVVQGKVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAALGKHIV 531

Query: 241 IYGGEVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNG 300
           I+GGE+    L H+G G+     + LDTE L+W R++   G E  P  RGW A +    G
Sbjct: 532 IFGGEIAMDPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIG 591

Query: 301 EELGLLVYGGNSPSNDRLGDIYFF 321
            + GL+++GG +P+NDR  D++F+
Sbjct: 592 GKKGLVMHGGKAPTNDRFDDLFFY 614

[Close]

BLAST of Bhi10M001012 vs. TAIR 10
Match: AT3G16400.1 (nitrile specifier protein 1 )

HSP 1 Score: 328.2 bits (840), Expect = 7.6e-90
Identity = 150/316 (47.47%), Postives = 204/316 (64.56%), Query Frame = 0


Query: 6   GKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIWTVAVV 65
           GKW K++QK  GPG R SH I  V  K Y+FGGEF+P  P+D  L+VFDL    W+++  
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDVPP-PRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSGPPHR 125
           +GDVP    +GV M SVG  +YVFGGRD+   + N  YSFDT+ ++W LL+  + GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDASR-QYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLAVAQD 185
           S+HS  AD+  VY+FGG   + RLN L +Y+ VD KW      G+ L  RGG GL V Q 
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVHY+D  Q++W QVET G +P+ RSVF + AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELGLLVY 305
             L H+G G+     + LDTE L+W R++   G E  P  RGW A +      + GL+++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465

[Close]

BLAST of Bhi10M001012 vs. TAIR 10
Match: AT3G16400.2 (nitrile specifier protein 1 )

HSP 1 Score: 328.2 bits (840), Expect = 7.6e-90
Identity = 150/316 (47.47%), Postives = 204/316 (64.56%), Query Frame = 0


Query: 6   GKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIWTVAVV 65
           GKW K++QK  GPG R SH I  V  K Y+FGGEF+P  P+D  L+VFDL    W+++  
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDVPP-PRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSGPPHR 125
           +GDVP    +GV M SVG  +YVFGGRD+   + N  YSFDT+ ++W LL+  + GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDASR-QYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLAVAQD 185
           S+HS  AD+  VY+FGG   + RLN L +Y+ VD KW      G+ L  RGG GL V Q 
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVHY+D  Q++W QVET G +P+ RSVF + AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELGLLVY 305
             L H+G G+     + LDTE L+W R++   G E  P  RGW A +      + GL+++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465

[Close]

BLAST of Bhi10M001012 vs. ExPASy Swiss-Prot
Match: Q93XW5 (Nitrile-specifier protein 5 OS=Arabidopsis thaliana OX=3702 GN=NSP5 PE=2 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 6.5e-102
Identity = 171/320 (53.44%), Postives = 218/320 (68.12%), Query Frame = 0


Query: 1   MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIW 60
           M P   KW K+ QK AGPGARSSHA+T+V  K Y FGGE  P + +DN L+VFDL    W
Sbjct: 1   MCPVENKWLKVGQKGAGPGARSSHAMTVVGNKVYCFGGELKPTIHIDNDLYVFDLETQEW 60

Query: 61  TVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSG 120
           ++A  +G+ P P  GV+M ++G  IYV+GGRD +    N L+S+DT  ++W LL+  + G
Sbjct: 61  SIAPATGEAPFPCFGVSMVTIGSTIYVYGGRDDKR-RYNGLHSYDTETNEWKLLAPVEEG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLA 180
            P RSYHS   DDR+VY+FGG    GR+N L AYD VD KW+EYPA GE  KGRG PGL 
Sbjct: 121 LPGRSYHSMAGDDRKVYVFGGVTAKGRVNTLHAYDVVDQKWVEYPAAGEACKGRGAPGLV 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           V + +IWV++GF G E+ D+H FD+A  QW  VET+G+ P ARSVF   + GKY+ IYGG
Sbjct: 181 VVEGRIWVLFGFDGNELGDIHCFDLASEQWKAVETTGDVPAARSVFPAVSYGKYIVIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELG 300
           E +P +L H+GAGK +GEVY LDTE L W R+  G   E  P  RGWCAF+      E G
Sbjct: 241 EEEPHELMHMGAGKMSGEVYQLDTETLVWERIVCG-NEEEKPSQRGWCAFTKAVKDGEEG 300

Query: 301 LLVYGGNSPSNDRLGDIYFF 321
           LLV+GGNSP+N+RL D+ F+
Sbjct: 301 LLVHGGNSPTNERLDDLVFW 318

[Close]

BLAST of Bhi10M001012 vs. ExPASy Swiss-Prot
Match: O04316 (Nitrile-specifier protein 4 OS=Arabidopsis thaliana OX=3702 GN=NSP4 PE=2 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 3.7e-89
Identity = 149/324 (45.99%), Postives = 210/324 (64.81%), Query Frame = 0


Query: 1   MAPT---NGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHE 60
           ++PT   +GKWTK+D+   GPG R SH I  V  K Y+FGGEF+P  P+D  L+VFD+  
Sbjct: 292 LSPTPNLHGKWTKVDENGDGPGLRCSHDIAQVGNKIYSFGGEFTPNQPIDKHLYVFDIES 351

Query: 61  HIWTVAVVSGDVPP-PRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSG 120
             W+++  +GD+P    +GV M S+G  +YVFGGRD+   + N  YSFDT+ ++W LL+ 
Sbjct: 352 RTWSISPATGDIPTLSCLGVCMVSIGSTLYVFGGRDASR-QYNGFYSFDTTTNEWKLLTP 411

Query: 121 SDSGPPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGG 180
            + GP  RS+HS  AD+  VY+FGG   + RLN L +Y+ VD KW      G+ L  RGG
Sbjct: 412 VEEGPTPRSFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGG 471

Query: 181 PGLAVAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVY 240
            GL V Q K+WVVYGF G E+DDVHY+D  Q++W QVET G +P+ RSVF + A+GK++ 
Sbjct: 472 AGLEVVQGKVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAALGKHIV 531

Query: 241 IYGGEVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNG 300
           I+GGE+    L H+G G+     + LDTE L+W R++   G E  P  RGW A +    G
Sbjct: 532 IFGGEIAMDPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIG 591

Query: 301 EELGLLVYGGNSPSNDRLGDIYFF 321
            + GL+++GG +P+NDR  D++F+
Sbjct: 592 GKKGLVMHGGKAPTNDRFDDLFFY 614

[Close]

BLAST of Bhi10M001012 vs. ExPASy Swiss-Prot
Match: Q9SDM9 (Nitrile-specifier protein 1 OS=Arabidopsis thaliana OX=3702 GN=NSP1 PE=1 SV=2)

HSP 1 Score: 328.2 bits (840), Expect = 1.1e-88
Identity = 150/316 (47.47%), Postives = 204/316 (64.56%), Query Frame = 0


Query: 6   GKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIWTVAVV 65
           GKW K++QK  GPG R SH I  V  K Y+FGGEF+P  P+D  L+VFDL    W+++  
Sbjct: 151 GKWIKVEQKGEGPGLRCSHGIAQVGNKIYSFGGEFTPNQPIDKHLYVFDLETRTWSISPA 210

Query: 66  SGDVPP-PRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSGPPHR 125
           +GDVP    +GV M SVG  +YVFGGRD+   + N  YSFDT+ ++W LL+  + GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYVFGGRDASR-QYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLAVAQD 185
           S+HS  AD+  VY+FGG   + RLN L +Y+ VD KW      G+ L  RGG GL V Q 
Sbjct: 271 SFHSMAADEENVYVFGGVSATARLNTLDSYNIVDKKWFHCSTPGDSLTARGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVHY+D  Q++W QVET G +P+ RSVF + AIGK++ I+GGE+  
Sbjct: 331 KVWVVYGFNGCEVDDVHYYDPVQDKWTQVETFGVRPSERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELGLLVY 305
             L H+G G+     + LDTE L+W R++   G E  P  RGW A +      + GL+++
Sbjct: 391 DPLAHVGPGQLTDGTFALDTETLQWERLDKFGGEEETPSSRGWTASTTATIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 465

[Close]

BLAST of Bhi10M001012 vs. ExPASy Swiss-Prot
Match: O49326 (Nitrile-specifier protein 2 OS=Arabidopsis thaliana OX=3702 GN=NSP2 PE=2 SV=1)

HSP 1 Score: 315.8 bits (808), Expect = 5.5e-85
Identity = 149/317 (47.00%), Postives = 205/317 (64.67%), Query Frame = 0


Query: 6   GKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIWTVAVV 65
           GKW K++QK  GPG R SH I  V  K ++FGGE +P  P+D  L+VFDL    W+++  
Sbjct: 152 GKWIKVEQKGEGPGPRCSHDIAQVGNKIFSFGGELTPNQPIDKHLYVFDLETRTWSISPA 211

Query: 66  SGDVPP-PRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSGPPHR 125
           +GDVP    +GV M S+G  +YVFGGRD+   + N  YSFDT+ ++W LL+  + GP  R
Sbjct: 212 TGDVPNLSCLGVRMVSIGSSLYVFGGRDASR-KYNGFYSFDTTKNEWKLLTPVEQGPTPR 271

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLAVAQD 185
           S+HS TAD+  VY+FGG   + RL  L AY+ VD KW++    G     RGG GL V Q 
Sbjct: 272 SFHSMTADENNVYVFGGVSATVRLKTLDAYNIVDHKWVQCSTPGGSCSVRGGAGLEVVQG 331

Query: 186 KIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGGEVDP 245
           K+WVVYGF G E+DDVH +D AQ++W QVET GEKP ARSVF +  +GK++ ++GGE+  
Sbjct: 332 KVWVVYGFNGCEVDDVHCYDPAQDKWTQVETFGEKPCARSVFASAVVGKHILVFGGEIAM 391

Query: 246 SDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENH-PGPRGWCAFSVGRNGEELGLLV 305
               H G G+ +G  + LDTE L+W ++ D LG E   P  RGW A + G    + GL++
Sbjct: 392 DPKAHEGPGQLSGGTFALDTETLKWEKL-DKLGEEEETPSIRGWSASTTGTIDGKKGLVM 451

Query: 306 YGGNSPSNDRLGDIYFF 321
           +GG + +NDR GD++F+
Sbjct: 452 FGGKAQTNDRFGDLFFY 466

[Close]

BLAST of Bhi10M001012 vs. ExPASy Swiss-Prot
Match: O04318 (Nitrile-specifier protein 3 OS=Arabidopsis thaliana OX=3702 GN=NSP3 PE=2 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 4.4e-82
Identity = 142/316 (44.94%), Postives = 194/316 (61.39%), Query Frame = 0


Query: 6   GKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIWTVAVV 65
           G W K++Q   GPG R SH I  V  K Y+FGGE  P  P+D  L+VFDL    W++A  
Sbjct: 151 GNWIKVEQNGEGPGLRCSHGIAQVGNKIYSFGGELIPNQPIDKHLYVFDLETRTWSIAPA 210

Query: 66  SGDVPP-PRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSGPPHR 125
           +GDVP    +GV M SVG  +Y FGGRD    + N  YSFDT+ ++W LL+  + GP  R
Sbjct: 211 TGDVPHLSCLGVRMVSVGSTLYTFGGRDFSR-QYNGFYSFDTTTNEWKLLTPVEEGPTPR 270

Query: 126 SYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLAVAQD 185
           S+HS  AD+  VY+FGG G   R+  L +Y+ VD  W      G+    RGG GL V Q 
Sbjct: 271 SFHSMAADEENVYVFGGVGAMDRIKTLDSYNIVDKTWFHCSNPGDSFSIRGGAGLEVVQG 330

Query: 186 KIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGGEVDP 245
           K+W+VYGF G E+DDVH++D A+++W QVET G KP  RSVF + AIGK++ I+GGE+  
Sbjct: 331 KVWIVYGFNGCEVDDVHFYDPAEDKWTQVETFGVKPNERSVFASAAIGKHIVIFGGEIAM 390

Query: 246 SDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELGLLVY 305
               H+G G+     + LDTE L+W R++     E  P  RGW A + G    + GL+++
Sbjct: 391 DPRAHVGPGQLIDGTFALDTETLQWERLDK---FEGTPSSRGWTASTTGTIDGKKGLVMH 450

Query: 306 GGNSPSNDRLGDIYFF 321
           GG +P+NDR  D++F+
Sbjct: 451 GGKAPTNDRFDDLFFY 462

[Close]

BLAST of Bhi10M001012 vs. NCBI nr
Match: XP_038900567.1 (nitrile-specifier protein 5 [Benincasa hispida])

HSP 1 Score: 695.7 bits (1794), Expect = 1.9e-196
Identity = 324/324 (100.00%), Postives = 324/324 (100.00%), Query Frame = 0


Query: 1   MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIW 60
           MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIW
Sbjct: 1   MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIW 60

Query: 61  TVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSG 120
           TVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSG
Sbjct: 61  TVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLA 180
           PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELG 300
           EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAPCE 325
           LLVYGGNSPSNDRLGDIYFFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAPCE 324

[Close]

BLAST of Bhi10M001012 vs. NCBI nr
Match: XP_008464157.1 (PREDICTED: nitrile-specifier protein 5 [Cucumis melo] >XP_016903161.1 PREDICTED: nitrile-specifier protein 5 [Cucumis melo] >KAA0060815.1 nitrile-specifier protein 5 [Cucumis melo var. makuwa] >TYK08820.1 nitrile-specifier protein 5 [Cucumis melo var. makuwa])

HSP 1 Score: 634.8 bits (1636), Expect = 4.1e-178
Identity = 288/324 (88.89%), Postives = 305/324 (94.14%), Query Frame = 0


Query: 1   MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIW 60
           MAPTNGKW KIDQKT GPGARSSHAI IV QKAY+FGGEFSPR+PVDNKLHVFDLH+HIW
Sbjct: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60

Query: 61  TVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSG 120
           TV  VSGD+PPPRVGVTMAS+G+IIYVFGGRD+EH ELNELYSFDTS +KWTLLSG +SG
Sbjct: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLA 180
           PPHRSYHSTTADD  VYIFGGCGNSGRLNDLWAYDTVDGKW+EYPA GE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYFD  QN+WAQVET G++PTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTE LEWSRV+DGLG+E+HPGPRGWCAFSVGR G ELG
Sbjct: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAPCE 325
           LLVYGGNSPSNDRLGDIYFFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAPCE 324

[Close]

BLAST of Bhi10M001012 vs. NCBI nr
Match: XP_004139996.1 (nitrile-specifier protein 5 [Cucumis sativus] >XP_004139997.1 nitrile-specifier protein 5 [Cucumis sativus] >XP_004139998.1 nitrile-specifier protein 5 [Cucumis sativus] >XP_011656888.1 nitrile-specifier protein 5 [Cucumis sativus] >KGN46612.1 hypothetical protein Csa_004873 [Cucumis sativus])

HSP 1 Score: 625.5 bits (1612), Expect = 2.5e-175
Identity = 283/324 (87.35%), Postives = 300/324 (92.59%), Query Frame = 0


Query: 1   MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIW 60
           MAPT G W KIDQKT GPGARSSHAI IV QKAY+FGGEFSPR+PVDNKLH+FDLH+HIW
Sbjct: 1   MAPTKGNWIKIDQKTTGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHIFDLHDHIW 60

Query: 61  TVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSG 120
           TV   SGD+PPPRVGVTMASVG+IIYVFGGRD+EH ELNELYSFDTS +KWTLLSG D G
Sbjct: 61  TVGAASGDIPPPRVGVTMASVGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGDYG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLA 180
           PP RSYHSTTADDRR+YIFGGCGNSGRLNDLWAYDT+DGKW+EYPA GE LKGRGGPGLA
Sbjct: 121 PPQRSYHSTTADDRRIYIFGGCGNSGRLNDLWAYDTIDGKWIEYPAAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYF   QN+W QVET GE+PTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFSPEQNKWTQVETKGERPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELG 300
           EVDPSDLGHLGAGKF GEVYVLDTEALEWSRVEDGLG+E+HPGPRGWCAF+VGR G ELG
Sbjct: 241 EVDPSDLGHLGAGKFVGEVYVLDTEALEWSRVEDGLGLEDHPGPRGWCAFAVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAPCE 325
           LLVYGGNSPSNDRLGDI+FFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIHFFAPCE 324

[Close]

BLAST of Bhi10M001012 vs. NCBI nr
Match: XP_023533621.1 (nitrile-specifier protein 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 623.2 bits (1606), Expect = 1.2e-174
Identity = 286/322 (88.82%), Postives = 302/322 (93.79%), Query Frame = 0


Query: 1   MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIW 60
           MAP NGKWTKIDQ  AGPGARSSHAITIV +KAYAFGGEFSPR+PVDN+LHVFDL EH W
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITIVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSG 120
           TVA VSGDVPPPRVGVTMA+VG+I+YVFGGRD EH ELNELYS DTS +KWTLLSG +SG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSLDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLA 180
           PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDT++GKW+EYP+ GE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFD AQN+WAQVET+GE+PTARSVF TCAIGKYVYIYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELG 300
           EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLG+ENHPGPRGWCAFSVGR G ELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWCAFSVGRKGRELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAP 323
           LLVYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

[Close]

BLAST of Bhi10M001012 vs. NCBI nr
Match: XP_022971053.1 (nitrile-specifier protein 5 [Cucurbita maxima])

HSP 1 Score: 623.2 bits (1606), Expect = 1.2e-174
Identity = 287/322 (89.13%), Postives = 301/322 (93.48%), Query Frame = 0


Query: 1   MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIW 60
           MAP NGKWTKIDQ  AGPGARSSHAITIV +KAYAFGGEFSPR+PVDNKLHVFDL EH W
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITIVGKKAYAFGGEFSPRIPVDNKLHVFDLDEHTW 60

Query: 61  TVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSG 120
           TVA VSGDVPPPRVGVTM +VG+I+YVFGGRD EH ELNELYSFDTS +KWTLLSG +SG
Sbjct: 61  TVASVSGDVPPPRVGVTMDAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLA 180
           PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDT+DGKW+EYP+ GE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIDGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFD AQN+WAQVET+GE+PT RSVF TCAIGKYVYIYGG
Sbjct: 181 VAQRKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTPRSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELG 300
           EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLG+ENHPGPRGWCAFSVGR G ELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAP 323
           LLVYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

[Close]

BLAST of Bhi10M001012 vs. ExPASy TrEMBL
Match: A0A1S3CLA8 (nitrile-specifier protein 5 OS=Cucumis melo OX=3656 GN=LOC103502104 PE=4 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.0e-178
Identity = 288/324 (88.89%), Postives = 305/324 (94.14%), Query Frame = 0


Query: 1   MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIW 60
           MAPTNGKW KIDQKT GPGARSSHAI IV QKAY+FGGEFSPR+PVDNKLHVFDLH+HIW
Sbjct: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60

Query: 61  TVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSG 120
           TV  VSGD+PPPRVGVTMAS+G+IIYVFGGRD+EH ELNELYSFDTS +KWTLLSG +SG
Sbjct: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLA 180
           PPHRSYHSTTADD  VYIFGGCGNSGRLNDLWAYDTVDGKW+EYPA GE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYFD  QN+WAQVET G++PTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTE LEWSRV+DGLG+E+HPGPRGWCAFSVGR G ELG
Sbjct: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAPCE 325
           LLVYGGNSPSNDRLGDIYFFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAPCE 324

[Close]

BLAST of Bhi10M001012 vs. ExPASy TrEMBL
Match: A0A5D3CBW1 (Nitrile-specifier protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold796G00270 PE=4 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.0e-178
Identity = 288/324 (88.89%), Postives = 305/324 (94.14%), Query Frame = 0


Query: 1   MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIW 60
           MAPTNGKW KIDQKT GPGARSSHAI IV QKAY+FGGEFSPR+PVDNKLHVFDLH+HIW
Sbjct: 1   MAPTNGKWIKIDQKTIGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHVFDLHDHIW 60

Query: 61  TVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSG 120
           TV  VSGD+PPPRVGVTMAS+G+IIYVFGGRD+EH ELNELYSFDTS +KWTLLSG +SG
Sbjct: 61  TVVAVSGDIPPPRVGVTMASIGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLA 180
           PPHRSYHSTTADD  VYIFGGCGNSGRLNDLWAYDTVDGKW+EYPA GE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDHNVYIFGGCGNSGRLNDLWAYDTVDGKWIEYPAAGEELKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYFD  QN+WAQVET G++PTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFDPDQNKWAQVETRGQRPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTE LEWSRV+DGLG+E+HPGPRGWCAFSVGR G ELG
Sbjct: 241 EVDPSDVGHLGAGKFAGEVYVLDTEVLEWSRVDDGLGVEDHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAPCE 325
           LLVYGGNSPSNDRLGDIYFFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFAPCE 324

[Close]

BLAST of Bhi10M001012 vs. ExPASy TrEMBL
Match: A0A0A0KDY9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G112500 PE=4 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.2e-175
Identity = 283/324 (87.35%), Postives = 300/324 (92.59%), Query Frame = 0


Query: 1   MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIW 60
           MAPT G W KIDQKT GPGARSSHAI IV QKAY+FGGEFSPR+PVDNKLH+FDLH+HIW
Sbjct: 1   MAPTKGNWIKIDQKTTGPGARSSHAIAIVDQKAYSFGGEFSPRLPVDNKLHIFDLHDHIW 60

Query: 61  TVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSG 120
           TV   SGD+PPPRVGVTMASVG+IIYVFGGRD+EH ELNELYSFDTS +KWTLLSG D G
Sbjct: 61  TVGAASGDIPPPRVGVTMASVGQIIYVFGGRDTEHKELNELYSFDTSINKWTLLSGGDYG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLA 180
           PP RSYHSTTADDRR+YIFGGCGNSGRLNDLWAYDT+DGKW+EYPA GE LKGRGGPGLA
Sbjct: 121 PPQRSYHSTTADDRRIYIFGGCGNSGRLNDLWAYDTIDGKWIEYPAAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQDKIWVVYGFAGMEMDDVHYF   QN+W QVET GE+PTARSVFCTCAIGKYVYIYGG
Sbjct: 181 VAQDKIWVVYGFAGMEMDDVHYFSPEQNKWTQVETKGERPTARSVFCTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELG 300
           EVDPSDLGHLGAGKF GEVYVLDTEALEWSRVEDGLG+E+HPGPRGWCAF+VGR G ELG
Sbjct: 241 EVDPSDLGHLGAGKFVGEVYVLDTEALEWSRVEDGLGLEDHPGPRGWCAFAVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAPCE 325
           LLVYGGNSPSNDRLGDI+FFAPCE
Sbjct: 301 LLVYGGNSPSNDRLGDIHFFAPCE 324

[Close]

BLAST of Bhi10M001012 vs. ExPASy TrEMBL
Match: A0A6J1I5Q1 (nitrile-specifier protein 5 OS=Cucurbita maxima OX=3661 GN=LOC111469839 PE=4 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 5.9e-175
Identity = 287/322 (89.13%), Postives = 301/322 (93.48%), Query Frame = 0


Query: 1   MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIW 60
           MAP NGKWTKIDQ  AGPGARSSHAITIV +KAYAFGGEFSPR+PVDNKLHVFDL EH W
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITIVGKKAYAFGGEFSPRIPVDNKLHVFDLDEHTW 60

Query: 61  TVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSG 120
           TVA VSGDVPPPRVGVTM +VG+I+YVFGGRD EH ELNELYSFDTS +KWTLLSG +SG
Sbjct: 61  TVASVSGDVPPPRVGVTMDAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLA 180
           PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDT+DGKW+EYP+ GE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIDGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFD AQN+WAQVET+GE+PT RSVF TCAIGKYVYIYGG
Sbjct: 181 VAQRKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTPRSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELG 300
           EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLG+ENHPGPRGWCAFSVGR G ELG
Sbjct: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWCAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAP 323
           LLVYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

[Close]

BLAST of Bhi10M001012 vs. ExPASy TrEMBL
Match: A0A6J1G6U4 (nitrile-specifier protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111451365 PE=4 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 6.6e-174
Identity = 284/322 (88.20%), Postives = 302/322 (93.79%), Query Frame = 0


Query: 1   MAPTNGKWTKIDQKTAGPGARSSHAITIVAQKAYAFGGEFSPRVPVDNKLHVFDLHEHIW 60
           MAP NGKWTKIDQ  AGPGARSSHAIT+V +KAYAFGGEFSPR+PVDN+LHVFDL EH W
Sbjct: 1   MAPMNGKWTKIDQNGAGPGARSSHAITVVGKKAYAFGGEFSPRIPVDNELHVFDLDEHTW 60

Query: 61  TVAVVSGDVPPPRVGVTMASVGRIIYVFGGRDSEHTELNELYSFDTSNSKWTLLSGSDSG 120
           TVA VSGDVPPPRVGVTMA+VG+I+YVFGGRD EH ELNELYSFDTS +KWTLLSG +SG
Sbjct: 61  TVASVSGDVPPPRVGVTMAAVGQIVYVFGGRDLEHKELNELYSFDTSINKWTLLSGGESG 120

Query: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTVDGKWMEYPAGGERLKGRGGPGLA 180
           PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDT++GKW+EYP+ GE LKGRGGPGLA
Sbjct: 121 PPHRSYHSTTADDRRVYIFGGCGNSGRLNDLWAYDTIEGKWIEYPSAGEGLKGRGGPGLA 180

Query: 181 VAQDKIWVVYGFAGMEMDDVHYFDVAQNQWAQVETSGEKPTARSVFCTCAIGKYVYIYGG 240
           VAQ KIWVVYGFAGMEMDDVHYFD AQN+WAQVET+GE+PTARSVF TCAIGKYVYIYGG
Sbjct: 181 VAQGKIWVVYGFAGMEMDDVHYFDPAQNKWAQVETTGERPTARSVFSTCAIGKYVYIYGG 240

Query: 241 EVDPSDLGHLGAGKFAGEVYVLDTEALEWSRVEDGLGMENHPGPRGWCAFSVGRNGEELG 300
           EVDPSD+GHLGAGKFAGEVYVLDTEALEWSRVEDGLG+ENHPGPRGW AFSVGR G ELG
Sbjct: 241 EVDPSDMGHLGAGKFAGEVYVLDTEALEWSRVEDGLGLENHPGPRGWSAFSVGRKGGELG 300

Query: 301 LLVYGGNSPSNDRLGDIYFFAP 323
           LLVYGGNSPSNDRLGDIYFF P
Sbjct: 301 LLVYGGNSPSNDRLGDIYFFTP 322

The following BLAST results are available for this feature:

BLAST of Bhi10M001012 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G07720.1	2.1e-132	65.31	Galactose oxidase/kelch repeat superfamily protein	[more]
AT5G48180.1	4.6e-103	53.44	nitrile specifier protein 5	[more]
AT3G16410.1	2.6e-90	45.99	nitrile specifier protein 4	[more]
AT3G16400.1	7.6e-90	47.47	nitrile specifier protein 1	[more]
AT3G16400.2	7.6e-90	47.47	nitrile specifier protein 1	[more]

BLAST of Bhi10M001012 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93XW5	6.5e-102	53.44	Nitrile-specifier protein 5 OS=Arabidopsis thaliana OX=3702 GN=NSP5 PE=2 SV=1	[more]
O04316	3.7e-89	45.99	Nitrile-specifier protein 4 OS=Arabidopsis thaliana OX=3702 GN=NSP4 PE=2 SV=1	[more]
Q9SDM9	1.1e-88	47.47	Nitrile-specifier protein 1 OS=Arabidopsis thaliana OX=3702 GN=NSP1 PE=1 SV=2	[more]
O49326	5.5e-85	47.00	Nitrile-specifier protein 2 OS=Arabidopsis thaliana OX=3702 GN=NSP2 PE=2 SV=1	[more]
O04318	4.4e-82	44.94	Nitrile-specifier protein 3 OS=Arabidopsis thaliana OX=3702 GN=NSP3 PE=2 SV=1	[more]

BLAST of Bhi10M001012 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038900567.1	1.9e-196	100.00	nitrile-specifier protein 5 [Benincasa hispida]	[more]
XP_008464157.1	4.1e-178	88.89	PREDICTED: nitrile-specifier protein 5 [Cucumis melo] >XP_016903161.1 PREDICTED:...	[more]
XP_004139996.1	2.5e-175	87.35	nitrile-specifier protein 5 [Cucumis sativus] >XP_004139997.1 nitrile-specifier ...	[more]
XP_023533621.1	1.2e-174	88.82	nitrile-specifier protein 5 [Cucurbita pepo subsp. pepo]	[more]
XP_022971053.1	1.2e-174	89.13	nitrile-specifier protein 5 [Cucurbita maxima]	[more]

BLAST of Bhi10M001012 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A1S3CLA8	2.0e-178	88.89	nitrile-specifier protein 5 OS=Cucumis melo OX=3656 GN=LOC103502104 PE=4 SV=1	[more]
A0A5D3CBW1	2.0e-178	88.89	Nitrile-specifier protein 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A0A0KDY9	1.2e-175	87.35	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G112500 PE=4 SV=1	[more]
A0A6J1I5Q1	5.9e-175	89.13	nitrile-specifier protein 5 OS=Cucurbita maxima OX=3661 GN=LOC111469839 PE=4 SV=...	[more]
A0A6J1G6U4	6.6e-174	88.20	nitrile-specifier protein 5 OS=Cucurbita moschata OX=3662 GN=LOC111451365 PE=4 S...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR015915	Kelch-type beta propeller	GENE3D	2.120.10.80		coord: 1..168 e-value: 7.0E-38 score: 132.4
IPR015915	Kelch-type beta propeller	GENE3D	2.120.10.80		coord: 169..324 e-value: 1.4E-20 score: 75.5
IPR015915	Kelch-type beta propeller	SUPERFAMILY	117281	Kelch motif	coord: 3..289
IPR006652	Kelch repeat type 1	PFAM	PF01344	Kelch_1	coord: 174..213 e-value: 4.5E-5 score: 22.9 coord: 72..114 e-value: 1.4E-9 score: 37.4
None	No IPR available	PFAM	PF13418	Kelch_4	coord: 123..161 e-value: 6.3E-13 score: 48.5 coord: 222..274 e-value: 7.0E-5 score: 22.8
None	No IPR available	PANTHER	PTHR47435	KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)	coord: 5..322
None	No IPR available	PANTHER	PTHR47435:SF4	KELCH REPEAT PROTEIN (AFU_ORTHOLOGUE AFUA_5G12780)	coord: 5..322

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Bhi10G001012	Bhi10G001012	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi10M001012.utr5p1	Bhi10M001012.utr5p1	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi10M001012.exon1	Bhi10M001012.exon1	exon
Bhi10M001012.exon2	Bhi10M001012.exon2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Bhi10M001012	cds.Bhi10M001012	CDS
cds.Bhi10M001012	cds.Bhi10M001012_2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi10M001012.utr3p1	Bhi10M001012.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Bhi10M001012	Bhi10M001012-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0080028	nitrile biosynthetic process
biological_process	GO:0050790	regulation of catalytic activity
biological_process	GO:0010043	response to zinc ion
cellular_component	GO:0005634	nucleus
molecular_function	GO:0005515	protein binding
molecular_function	GO:0030234	enzyme regulator activity