Bhi10M000508 (mRNA) Wax gourd (B227) v1

Overview
NameBhi10M000508
TypemRNA
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionLaccase
Locationchr10: 13642571 .. 13647367 (-)
Sequence length1434
RNA-Seq ExpressionBhi10M000508
SyntenyBhi10M000508
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTGTTTCTCTTACATCACAGCATGATTTTATGAGATTCTTTTTTCTAACATTTCTTGTATCTCTCATCTCTAAACATGCACTTGCAATAACCAGGCATTATCAATTTAATGTAATAATGTTTATTTCTCATGCAGATTAAAATTGAAGGCCTTGAATTTATGCATTTTATTATATTATAATGTTTATTTTCTAATTTTTTGATGTATATTAATGCAGATCCAAATGCAAAGTGTAACAAGATTATGCCATTCCAAAAACATTGCGACAGTGAATGGGAAGTTCCCTGGGCCTTCTATTATAGTTACCGAAGGAGACGAGCTCGTCGTCAAAGTTGTTAACCGTATCCAAAACAATGTTACCATTCACTGGTGAGAAGATTATGTTACGAAAAATATGAAAAACAAAAAAACTTGAGTACAATCAATTGCACACCAATAAAAATATGTCATTTAACAATTTTTTTTACTAACAAAAATGGGATACATGAACTGCATCTAATCACTATCCCAAAGATATTAACTCTTAGGTGATCTTTTTTTGCTTCTATATTTTGTTTATTTGTTTAATAATAATATGCAAAGAATGAGATAGAATTAATGATTAGAGTGAGCTTATCTCAATGCTAATTAGCATGATCTTCCATTCAAGAGGTAGGAGATTTGATTATCCATTTTTATAATTATGCATGACCTTCTATTGAGCATTGTGACACAAATTTCAGAATTTTTTTATTGTTAGATAGTCTCTCGTGCATCTGATAATGCAAAACTGTTGATTGTATGGTAGTGGTCTTAACTTTATTTGTTTAAAAAAAAAAAAAAACTTTAGAATTTTGAAGAGAAACCGAAACCTATCTAAAATTGTGTGGCATTAATCTAAGAATGAAAATTTAAATCACTACCCATCCCAACTTCCTTTAGAAATTTTAAAGGATTCTAAAAAGGTTTATAAAAGACTTAAAGTAATATTACAACTTCGGAAAGAATAATAATATTTTTAAAAACAAAAGAAAAAAGTTCAATTTTGTTCCTTCCGTTTACCTAATTTTAGGGGTCACGTTAGAATTCATCAATTTAAGTTAGATTATTACTTTTTATTCTTTATAAAGAAATTTGGATGGATCATCAGGTTCATCTATCTTTGTGCAGTTCATGTAAATTGGTCATTTGATAAGCTCTTATTTTTTGTTACTTGATTGAAGTAAGTTGAAAAAAAATGAGTGCAAGATGCAGCAAAAAATGTACCCAAATATTGGTATCTTTCAACGTTCACATTTTCACTCTTTTAGAAGTTAATTAGACGTTAACAAAAAATAATTACAGGCATGGAATTAGACAATTCAGAACAGGTTGGGCAGATGGACCAGCATATATAACACAATGTCCAATTCAACCTGGCCAAAGTTACAACTATAAGTTCACCATTATTGGGCAAAGAGGAACTCTATGGTGGCATGGTCACATTTCTTGGCTTAGAGCAACAGTTTATGGTCCTCTTATCATTCTTCCCAATAACCTTCCCCTTGCTTATCCATTTCCTAACCCATTCAAGGAAGTTCATCCCTTAATGTTTGGTAAGGTTGAATTATACAAATTACCATATCCTTCAACTTTGTACTATGCCCTTATTGTTAGTTTCTTATGGAAATTCTTACTACTTTGTTTCAATTCTATAGGTAGAAAAGGCTTTCAAATCTTAAAAACAAAAATTTAAAAATAATGGATTCAGTTATAATAGAGTTGTATTTTCAAATTTATGGATAGAAAGAATTTTAAAATCTAAAAACATAAATTTGAAAACAAGGGGTTCAGTGATAATAGAATCGTATTTTGTGTTTTCAAATATGTTTTTAGTAGAAAATTTAGAGATTGGATTTTAATTTAAATAATTATCTTACATGTCGTTGATATTATATTTATAAACCATAAAACTAATTGTAGTTCCACACTACATATTTGGTTGCCTGTAAACTATTTTCAATTAATTTATGAATATGTTTTATATTAAAAGAAACGTATGAAAATTATATAATATAAAGTAATTATTTTAAATAATAAAATTGTTGAAAATATTTTCAAGTATAATAAAATATCACTGTCTATCAGTGATGGACCGCGATAGACCACAATGGACATTTATAAGTGGACGATAGACCGTATAAAGACATCTATCAAATATTAATAGATGTTTATCGTTGTCTATAGTGGTCTATCACTGATAAGTAATGACATTTTCCTATATTTATAAATAAGTTGATTCATTTTTTCTTTATTTGAAAACGTCTCTAATTTAAATATATTACATAGTAAATAGTCTGAATTGAATTTATAACCTAAAATATTGTATTTCTAGATTTTATTTTTATTTAATATAACTTTACATTTTAAAATTTAAAATTCAAAATTTAGATATATTAAAAATATAAAAATATTGTTTTCATAATTTATATTATTTAGGTGACATAATCCAAAAACAAAAAACAAAATTCACGTGGTCTATTAAACACATATTTGCTAAACTATATATATTTGTGATTTGTTTTTTTTTTTAAGGAGAGTGGTGGAATGATGATACACAGGCAGTGATTACTCGAGCTCTTCAAAATGGTGGGAACCCTAATATCTCAGATGCCTTCACTATAAATGGCCTTCCTGGGCCATTCTATAATTGCTCTGCTCATGGTATGCAACTTTACAACAACTTACCTTACATTGTTTTGATAATTTCGGTCTCATTTGGTAATTATTTTATTTTTTTGTTTTTGTTTTTTAAAATTAAGCCTATGAACACTACTTCCACTTCCGAATTCCTTCATTTCTTATCTACTTTTCACCTATAGTTTAAAAAGCTAAGTCAAATTTTGAGAACTGAAAAAGGTAGTGTTCAAAAAGTTGTTTTTGTTTTTTAAATTTGATTAATATTTCAGCCATTGTATTTAAGAAATATACAAATTATTGTAAAAAATGTGGATGAAATGAGTTTAATTTTCAAAAAACAAATAGTTACTAAACATAATCTTCAAAATTAAAGTTTAGTTTTAGCTTGGTCTTTATAGATTTAAAAGTTTTGGTCAAATCATATTCACAAATAATGAGATGGTATATATTAGGTTTTAGATATTTTAGTATATATTAGGTTTTCGATATTTTAGTCTCTATATTTTGGGTTTTATTACATTTTAGGGTCTATTTAGAGGGATGAGAAGGAAAAAAAATGTTTTTCAAAAAATCATTTTTATTTAATCTCTTGTTCAAAATACATTTTAAGAATGTTTCAAAATCTATTTTTAGTATTTTGAATGGTTGTCAAATAGTTCATTGTACTTTCAATTATTCAATTGCAATACACGTATTTTCAATAAATTTCAAATTTAGTTATTGTATTTCCAATAAATATTTAATTTATTTAGTTAAATTAATTTTTTTTTATCAAAATTGGTTAAACAATAATAATAATTTTAATCAAAGGAAAGACACTATCCATATCCATATAAATTTATCTCTACAATTGTATATATTTGGGGATCCAATTTCTACCACTATTATAAGTTTTAGGATCCAATTTTAATACTTGTATAAGTTTGAGTACTTAATTTCTATAATTGAAAATTTGGGTGATGGTTGCAGATACTTACAAGCTGAAAGTAGAAGCAGGAAAGACATATCTTTTACGAATAATCAACGCTGCACTCAATGACGATTTGTTCTTCACGATCGCTAATCACACTCTAACAGTCGTAGAAGCCGACGCTAATTATGTCAAGCCATTCCAAACCAATATCATTCTTATAGCCCCTGGGCAAACCACAAACGTTCTTCTTCATACAAAACCCAACCCCCAAAACAACGACACGTTCTACATGCTCGCTCGCCCTTTCTCCACCGCGCAACCAGCCACATTCGACAATTCCACCGTTGGCGGAATTTTAGAGTATCACCACCAACAACAACAATCCACAACAGGTTTTGTTTACAAGCCAACGCTTCCGGCTATAAACGACACAGCATTTGCTGCAAACTTCACCAACAAAATCCGCAGCTTGACTAGCTTTAGTTTCCCCAAGAAAGTTGACAAGCGATTCTTTTTCACAGTGGGCCTTGGGACAAGCCCATGTAAGCGTGGCCCAAATCGGGCCTGTCAAGGGCCCAATGGGTCGAGGTTTGCGGCCTCTATAAACAACGTTTCTTTTTCTTTGCCTAATACGGCCCTTTTGCAGGCCCATTATTTGGGCCGGGCCAATGGGATTTATACGGACGACTTCCCTAACGGCCCAGAATATTTTTTCAACTTCACGGGCCCACCGTTGAACAACACGATGGTGAGAAATGGAACGAGGGCGGTATGGTTGGATTTTAATACAAGTGTGGAATTGATATTGCAGGGTACTAGTCTCGTTAATGCTGAGAATCATCCTTTTCATTTGCATGGGCACGATGTTTTTGTGGTTGGTCAAGGTTTTGGCAACTTTGACCCAAAAAAGGATCCTCAAAACTTCAATCTTGTTGACCCAATGCGAAGGAATACGTTCGGTGTTCCTTCAGGAGGATGGGTGGTTATTCGCTTTCTTGCTAATAATCCAGGTAGATAAAACCAAACCTTTTTAAGTTTTGTATCTGTCTCATTCTTTAACTTTTAAAAGTATCTAATATTAGAATGAACAGGCGTATGGCTAATGCATTGCCACTTGGAGGTGCACGTAGGCTGGGGCTTGGAGATGGTGTGGGTCGTCTCAGATGGGAAGTTCCCCGATCAGAAGTTGCGCCCTCCACCATCCGATCTTCCCAAGTGTTAG

mRNA sequence

ATGGGTATCCAAATGCAAAGTGTAACAAGATTATGCCATTCCAAAAACATTGCGACAGTGAATGGGAAGTTCCCTGGGCCTTCTATTATAGTTACCGAAGGAGACGAGCTCGTCGTCAAAGTTGTTAACCGTATCCAAAACAATGTTACCATTCACTGGCATGGAATTAGACAATTCAGAACAGGTTGGGCAGATGGACCAGCATATATAACACAATGTCCAATTCAACCTGGCCAAAGTTACAACTATAAGTTCACCATTATTGGGCAAAGAGGAACTCTATGGTGGCATGGTCACATTTCTTGGCTTAGAGCAACAGTTTATGGTCCTCTTATCATTCTTCCCAATAACCTTCCCCTTGCTTATCCATTTCCTAACCCATTCAAGGAAGTTCATCCCTTAATGTTTGGAGAGTGGTGGAATGATGATACACAGGCAGTGATTACTCGAGCTCTTCAAAATGGTGGGAACCCTAATATCTCAGATGCCTTCACTATAAATGGCCTTCCTGGGCCATTCTATAATTGCTCTGCTCATGTCGTAGAAGCCGACGCTAATTATGTCAAGCCATTCCAAACCAATATCATTCTTATAGCCCCTGGGCAAACCACAAACGTTCTTCTTCATACAAAACCCAACCCCCAAAACAACGACACGTTCTACATGCTCGCTCGCCCTTTCTCCACCGCGCAACCAGCCACATTCGACAATTCCACCGTTGGCGGAATTTTAGAGTATCACCACCAACAACAACAATCCACAACAGGTTTTGTTTACAAGCCAACGCTTCCGGCTATAAACGACACAGCATTTGCTGCAAACTTCACCAACAAAATCCGCAGCTTGACTAGCTTTAGTTTCCCCAAGAAAGTTGACAAGCGATTCTTTTTCACAGTGGGCCTTGGGACAAGCCCATGTAAGCGTGGCCCAAATCGGGCCTGTCAAGGGCCCAATGGGTCGAGGTTTGCGGCCTCTATAAACAACGTTTCTTTTTCTTTGCCTAATACGGCCCTTTTGCAGGCCCATTATTTGGGCCGGGCCAATGGGATTTATACGGACGACTTCCCTAACGGCCCAGAATATTTTTTCAACTTCACGGGCCCACCGTTGAACAACACGATGGTGAGAAATGGAACGAGGGCGGGTACTAGTCTCGTTAATGCTGAGAATCATCCTTTTCATTTGCATGGGCACGATGTTTTTGTGGTTGGTCAAGGTTTTGGCAACTTTGACCCAAAAAAGGATCCTCAAAACTTCAATCTTGTTGACCCAATGCGAAGGAATACGTTCGGTGTTCCTTCAGGAGGATGGGTGGTTATTCGCTTTCTTGCTAATAATCCAGGCTGGGGCTTGGAGATGGTGTGGGTCGTCTCAGATGGGAAGTTCCCCGATCAGAAGTTGCGCCCTCCACCATCCGATCTTCCCAAGTGTTAG

Coding sequence (CDS)

ATGGGTATCCAAATGCAAAGTGTAACAAGATTATGCCATTCCAAAAACATTGCGACAGTGAATGGGAAGTTCCCTGGGCCTTCTATTATAGTTACCGAAGGAGACGAGCTCGTCGTCAAAGTTGTTAACCGTATCCAAAACAATGTTACCATTCACTGGCATGGAATTAGACAATTCAGAACAGGTTGGGCAGATGGACCAGCATATATAACACAATGTCCAATTCAACCTGGCCAAAGTTACAACTATAAGTTCACCATTATTGGGCAAAGAGGAACTCTATGGTGGCATGGTCACATTTCTTGGCTTAGAGCAACAGTTTATGGTCCTCTTATCATTCTTCCCAATAACCTTCCCCTTGCTTATCCATTTCCTAACCCATTCAAGGAAGTTCATCCCTTAATGTTTGGAGAGTGGTGGAATGATGATACACAGGCAGTGATTACTCGAGCTCTTCAAAATGGTGGGAACCCTAATATCTCAGATGCCTTCACTATAAATGGCCTTCCTGGGCCATTCTATAATTGCTCTGCTCATGTCGTAGAAGCCGACGCTAATTATGTCAAGCCATTCCAAACCAATATCATTCTTATAGCCCCTGGGCAAACCACAAACGTTCTTCTTCATACAAAACCCAACCCCCAAAACAACGACACGTTCTACATGCTCGCTCGCCCTTTCTCCACCGCGCAACCAGCCACATTCGACAATTCCACCGTTGGCGGAATTTTAGAGTATCACCACCAACAACAACAATCCACAACAGGTTTTGTTTACAAGCCAACGCTTCCGGCTATAAACGACACAGCATTTGCTGCAAACTTCACCAACAAAATCCGCAGCTTGACTAGCTTTAGTTTCCCCAAGAAAGTTGACAAGCGATTCTTTTTCACAGTGGGCCTTGGGACAAGCCCATGTAAGCGTGGCCCAAATCGGGCCTGTCAAGGGCCCAATGGGTCGAGGTTTGCGGCCTCTATAAACAACGTTTCTTTTTCTTTGCCTAATACGGCCCTTTTGCAGGCCCATTATTTGGGCCGGGCCAATGGGATTTATACGGACGACTTCCCTAACGGCCCAGAATATTTTTTCAACTTCACGGGCCCACCGTTGAACAACACGATGGTGAGAAATGGAACGAGGGCGGGTACTAGTCTCGTTAATGCTGAGAATCATCCTTTTCATTTGCATGGGCACGATGTTTTTGTGGTTGGTCAAGGTTTTGGCAACTTTGACCCAAAAAAGGATCCTCAAAACTTCAATCTTGTTGACCCAATGCGAAGGAATACGTTCGGTGTTCCTTCAGGAGGATGGGTGGTTATTCGCTTTCTTGCTAATAATCCAGGCTGGGGCTTGGAGATGGTGTGGGTCGTCTCAGATGGGAAGTTCCCCGATCAGAAGTTGCGCCCTCCACCATCCGATCTTCCCAAGTGTTAG

Protein sequence

MGIQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTGWADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAYPFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAHVVEADANYVKPFQTNIILIAPGQTTNVLLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEYHHQQQQSTTGFVYKPTLPAINDTAFAANFTNKIRSLTSFSFPKKVDKRFFFTVGLGTSPCKRGPNRACQGPNGSRFAASINNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRNGTRAGTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVDPMRRNTFGVPSGGWVVIRFLANNPGWGLEMVWVVSDGKFPDQKLRPPPSDLPKC
Homology
BLAST of Bhi10M000508 vs. TAIR 10
Match: AT5G60020.1 (laccase 17 )

HSP 1 Score: 621.7 bits (1602), Expect = 4.9e-178
Identity = 312/551 (56.62%), Postives = 381/551 (69.15%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           I+MQ+VTRLCH+K++ +VNG+FPGP +I  EGD++++KVVN++ NN+++HWHGIRQ R+G
Sbjct: 31  IKMQNVTRLCHTKSLVSVNGQFPGPKLIAREGDQVLIKVVNQVPNNISLHWHGIRQLRSG 90

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGPAYITQCPIQ GQSY Y +TI+GQRGTLW+H HISWLR+TVYGPLIILP    + Y
Sbjct: 91  WADGPAYITQCPIQTGQSYVYNYTIVGQRGTLWYHAHISWLRSTVYGPLIILPKR-GVPY 150

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PF  P KEV P++FGEW+N DT+A+I +A Q GG PN+SDA+TINGLPGP YNCSA    
Sbjct: 151 PFAKPHKEV-PMIFGEWFNADTEAIIRQATQTGGGPNVSDAYTINGLPGPLYNCSAKDTF 210

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            VVEADA YVKPF+T  ILIAPGQTTNV
Sbjct: 211 RLRVKPGKTYLLRLINAALNDELFFSIANHTVTVVEADAIYVKPFETETILIAPGQTTNV 270

Query: 243 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEYHHQQQ----QSTTGF----V 302
           LL TK +   + +F+M ARP+ T Q  TFDNSTV GILEY   +Q     S T      +
Sbjct: 271 LLKTK-SSYPSASFFMTARPYVTGQ-GTFDNSTVAGILEYEPPKQTKGAHSRTSIKNLQL 330

Query: 303 YKPTLPAINDTAFAANFTNKIRSLTSFSFPK----KVDKRFFFTVGLGTSPCKRGPNRAC 362
           +KP LPA+NDT FA  F+NK+RSL S +FP      VD++FFFTVGLGT+PC    N+ C
Sbjct: 331 FKPILPALNDTNFATKFSNKLRSLNSKNFPANVPLNVDRKFFFTVGLGTNPCNHKNNQTC 390

Query: 363 QGP-NGSRFAASINNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNT 422
           QGP N + FAASI+N+SF++P  ALLQ+HY G+++G+Y+  FP  P   FN+TG P NNT
Sbjct: 391 QGPTNTTMFAASISNISFTMPTKALLQSHYSGQSHGVYSPKFPWSPIVPFNYTGTPPNNT 450

Query: 423 MVRNGTR--------------AGTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNF 478
           MV NGT                 TS++ AE+HP HLHG + FVVGQGFGNFDP KDP+NF
Sbjct: 451 MVSNGTNLMVLPYNTSVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPNKDPRNF 510

BLAST of Bhi10M000508 vs. TAIR 10
Match: AT2G29130.1 (laccase 2 )

HSP 1 Score: 609.4 bits (1570), Expect = 2.5e-174
Identity = 302/544 (55.51%), Postives = 368/544 (67.65%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           IQ++++TRLC +K I TVNGKFPGP +   EGD L +KVVN + NN++IHWHGIRQ R+G
Sbjct: 36  IQLKNITRLCKTKTIVTVNGKFPGPRVTAREGDNLQIKVVNHVSNNISIHWHGIRQLRSG 95

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGP+Y+TQCPI+ GQSY Y FT+ GQRGTLWWH HI W+RATVYGPLIILP  L   Y
Sbjct: 96  WADGPSYVTQCPIRMGQSYVYNFTVTGQRGTLWWHAHIQWMRATVYGPLIILP-KLHQPY 155

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PFP P+K+V P++FGEW+N D QAV+ +ALQ G  PN SDA T NGLPGP YNCS     
Sbjct: 156 PFPKPYKQV-PILFGEWFNADPQAVVQQALQTGAGPNASDAHTFNGLPGPLYNCSTKDTY 215

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            VVEADA YVKPFQTNI+L+ PGQTTNV
Sbjct: 216 KLMVKPGKTYLLRLINAALNDELFFTIANHTLTVVEADACYVKPFQTNIVLLGPGQTTNV 275

Query: 243 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEYHHQQQQSTTGFVYKPTLPAI 302
           LL TKP    N TFYMLARP+ T Q  T DN+TV GIL+Y H  + S    + KP+LP I
Sbjct: 276 LLKTKP-IYPNATFYMLARPYFTGQ-GTIDNTTVAGILQYQHHTKSSKNLSIIKPSLPPI 335

Query: 303 NDTAFAANFTNKIRSLTSFSF----PKKVDKRFFFTVGLGTSPCKRGPNRACQGP-NGSR 362
           N T++AANFT   RSL S +F    PK VDK++FF +GLGT+PC +  N+ CQGP N ++
Sbjct: 336 NSTSYAANFTKMFRSLASSTFPANVPKVVDKQYFFAIGLGTNPCPK--NQTCQGPTNTTK 395

Query: 363 FAASINNVSFSLPN-TALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRNGTR 422
           FAASINNVSF LPN T+LLQ++++G++  ++  DFP  P   FN+TG P NNTMV  GT+
Sbjct: 396 FAASINNVSFILPNKTSLLQSYFVGKSKNVFMTDFPTAPIIPFNYTGTPPNNTMVSRGTK 455

Query: 423 A--------------GTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVDPMR 478
                          GTS++  E HP HLHG + +VVGQGFGNF+P +DP+++NLVDP+ 
Sbjct: 456 VVVLKYKTTVELVLQGTSILGIEAHPIHLHGFNFYVVGQGFGNFNPARDPKHYNLVDPVE 515

BLAST of Bhi10M000508 vs. TAIR 10
Match: AT2G38080.1 (Laccase/Diphenol oxidase family protein )

HSP 1 Score: 491.9 bits (1265), Expect = 5.8e-139
Identity = 257/541 (47.50%), Postives = 319/541 (58.96%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           + M++VTRLC SK   TVNG++PGP+I   E D L++KVVN ++ NV+IHWHG+RQ RTG
Sbjct: 33  VVMKNVTRLCSSKPTVTVNGRYPGPTIYAREDDTLLIKVVNHVKYNVSIHWHGVRQVRTG 92

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGPAYITQCPIQPGQ Y Y +T+ GQRGTLWWH HI WLRATVYG L+ILP    + Y
Sbjct: 93  WADGPAYITQCPIQPGQVYTYNYTLTGQRGTLWWHAHILWLRATVYGALVILPKR-GVPY 152

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PFP P  E   ++ GEWW  DT+ +I  AL++G  PN+SD+  ING PGP  NC +    
Sbjct: 153 PFPKPDNE-KVIVLGEWWKSDTENIINEALKSGLAPNVSDSHMINGHPGPVRNCPSQGYK 212

Query: 183 --------------------------------VVEADANYVKPFQTNIILIAPGQTTNVL 242
                                           VVE DA YVKPF+T+ +LIAPGQTTNVL
Sbjct: 213 LSVENGKTYLLRLVNAALNEELFFKVAGHIFTVVEVDAVYVKPFKTDTVLIAPGQTTNVL 272

Query: 243 LHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEYHHQQQQSTTGFVYKPTLPAIN 302
           L      ++   + + A PF  A P   DN T    + Y      S T     P     N
Sbjct: 273 LTAS---KSAGKYLVTASPFMDA-PIAVDNVTATATVHYSGTLSSSPTILTLPPPQ---N 332

Query: 303 DTAFAANFTNKIRSLTSFSF----PKKVDKRFFFTVGLGTSPCKRGPNRACQGPNGSRFA 362
            T+ A NFTN +RSL S  +    P  +D   FFTVGLG + C       C+  NGSR  
Sbjct: 333 ATSIANNFTNSLRSLNSKKYPALVPTTIDHHLFFTVGLGLNACP-----TCKAGNGSRVV 392

Query: 363 ASINNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRNGTR--- 422
           ASINNV+F +P TALL AHY    +G++T DFP  P + FN++G  + N     GTR   
Sbjct: 393 ASINNVTFIMPKTALLPAHYF-NTSGVFTTDFPKNPPHVFNYSGGSVTNMATETGTRLYK 452

Query: 423 -----------AGTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVDPMRRNT 478
                        T ++  ENHP HLHG + F VG+G GNF+  KDP+NFNLVDP+ RNT
Sbjct: 453 LPYNATVQLVLQDTGVIAPENHPVHLHGFNFFEVGRGLGNFNSTKDPKNFNLVDPVERNT 512

BLAST of Bhi10M000508 vs. TAIR 10
Match: AT5G03260.1 (laccase 11 )

HSP 1 Score: 485.3 bits (1248), Expect = 5.5e-137
Identity = 250/543 (46.04%), Postives = 326/543 (60.04%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           +Q+++++R+C++K I TVNG FPGP++   EGD +++ V N +Q N++IHWHG++Q+R G
Sbjct: 32  VQVKNISRICNAKPIVTVNGMFPGPTVYAREGDRVIINVTNHVQYNMSIHWHGLKQYRNG 91

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGPAYITQCPIQ GQSY Y F + GQRGTLWWH HI WLRATVYG ++ILP      Y
Sbjct: 92  WADGPAYITQCPIQTGQSYLYDFNVTGQRGTLWWHAHILWLRATVYGAIVILPAP-GKPY 151

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PFP P++E + ++ GEWWN D +  + +A Q G  P +SDA TING PGP + CS     
Sbjct: 152 PFPQPYQESN-IILGEWWNKDVETAVNQANQLGAPPPMSDAHTINGKPGPLFPCSEKHTF 211

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            VVE DA Y KPF T  IL+ PGQTTNV
Sbjct: 212 VIEAEAGKTYLLRIINAALNDELFFGIAGHNMTVVEIDAVYTKPFTTKAILLGPGQTTNV 271

Query: 243 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEYHHQQQQSTTGFVYKPTLPAI 302
           L+ T  +P   + ++M A PF  A P + DN TV  IL+Y   +    T     P LP  
Sbjct: 272 LVKTDRSP---NRYFMAASPFMDA-PVSVDNKTVTAILQY---KGVPNTVLPILPKLPLP 331

Query: 303 NDTAFAANFTNKIRSLTSFSFPK----KVDKRFFFTVGLGTSPCKRGPNRACQGPNGSRF 362
           NDT+FA ++  K++SL + +FP     KVD+R F+T+GLG + C       C   NG+  
Sbjct: 332 NDTSFALDYNGKLKSLNTPNFPALVPLKVDRRLFYTIGLGINACP-----TC--VNGTNL 391

Query: 363 AASINNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLN-NTMVRNGTRA 422
           AASINN++F +P TALL+AHY    +G++  DFP+ P   FN+TG PL  N     GTR 
Sbjct: 392 AASINNITFIMPKTALLKAHY-SNISGVFRTDFPDRPPKAFNYTGVPLTANLGTSTGTRL 451

Query: 423 G--------------TSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVDPMRR 478
                          T+L+  E+HPFHLHG++ FVVG G GNFDPKKDP  FNLVDP  R
Sbjct: 452 SRVKFNTTIELVLQDTNLLTVESHPFHLHGYNFFVVGTGVGNFDPKKDPAKFNLVDPPER 511

BLAST of Bhi10M000508 vs. TAIR 10
Match: AT5G05390.1 (laccase 12 )

HSP 1 Score: 483.4 bits (1243), Expect = 2.1e-136
Identity = 254/542 (46.86%), Postives = 326/542 (60.15%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           IQ   V RLC ++N  TVNG FPGP++ V  GD L VKV NR + N+TIHWHG+RQ RTG
Sbjct: 33  IQETPVKRLCKTRNAITVNGMFPGPTLEVNNGDTLEVKVHNRARYNITIHWHGVRQIRTG 92

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGP ++TQCPI+PG+SY Y+FTI GQ GTLWWH H SWLRATVYG LII P     ++
Sbjct: 93  WADGPEFVTQCPIRPGKSYTYRFTIQGQEGTLWWHAHSSWLRATVYGALIIHPTP-GSSF 152

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PFP P ++   LM GEWWN +   VI +A + G  PNISDA+TING PG  YNCS     
Sbjct: 153 PFPKPDRQT-ALMLGEWWNANPVDVINQATRTGAAPNISDAYTINGQPGDLYNCSTKETV 212

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            VV ADA+Y+KPF T ++++ PGQTT+V
Sbjct: 213 VVPINSGETSLLRVINAALNQPLFFTVANHKLTVVGADASYLKPFTTKVLMLGPGQTTDV 272

Query: 243 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEYHHQQQQSTTGFVYKPTLPAI 302
           LL     P+    +Y+ AR + +AQ A FDN+T   IL+Y   ++ +TT     P LPA 
Sbjct: 273 LLTADQPPKR---YYIAARAYQSAQNAPFDNTTTTAILQY---KKTTTTSKPIMPVLPAF 332

Query: 303 NDTAFAANFTNKIRSLTSFSFPKKVDKRFFFTVGLGTSPC-KRGPNRACQGPNGSRFAAS 362
           NDT    +F+ K +SL +   PK +D   FFT+GLG   C K+ P   CQG NG+RF AS
Sbjct: 333 NDTNTVTSFSRKFKSLRNVVVPKTIDDNLFFTIGLGLDNCPKKFPKSRCQGLNGTRFTAS 392

Query: 363 INNVSFSLP-NTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTM---------- 422
           +NNVSF LP N +LLQAH  G   G++T DFP+ P   F++TG  ++  +          
Sbjct: 393 MNNVSFVLPSNFSLLQAHSNG-IPGVFTTDFPSKPPVKFDYTGNNISRALFQPVKGTKLY 452

Query: 423 -VRNGTRA-----GTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVDPMRRN 478
            ++ G+R       T++V +ENHP HLHG+D ++VG+GFGNF+PKKD   FNLVDP  RN
Sbjct: 453 KLKYGSRVQVVLQDTNIVTSENHPIHLHGYDFYIVGEGFGNFNPKKDTSKFNLVDPPLRN 512

BLAST of Bhi10M000508 vs. ExPASy Swiss-Prot
Match: Q9FJD5 (Laccase-17 OS=Arabidopsis thaliana OX=3702 GN=LAC17 PE=2 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 6.9e-177
Identity = 312/551 (56.62%), Postives = 381/551 (69.15%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           I+MQ+VTRLCH+K++ +VNG+FPGP +I  EGD++++KVVN++ NN+++HWHGIRQ R+G
Sbjct: 31  IKMQNVTRLCHTKSLVSVNGQFPGPKLIAREGDQVLIKVVNQVPNNISLHWHGIRQLRSG 90

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGPAYITQCPIQ GQSY Y +TI+GQRGTLW+H HISWLR+TVYGPLIILP    + Y
Sbjct: 91  WADGPAYITQCPIQTGQSYVYNYTIVGQRGTLWYHAHISWLRSTVYGPLIILPKR-GVPY 150

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PF  P KEV P++FGEW+N DT+A+I +A Q GG PN+SDA+TINGLPGP YNCSA    
Sbjct: 151 PFAKPHKEV-PMIFGEWFNADTEAIIRQATQTGGGPNVSDAYTINGLPGPLYNCSAKDTF 210

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            VVEADA YVKPF+T  ILIAPGQTTNV
Sbjct: 211 RLRVKPGKTYLLRLINAALNDELFFSIANHTVTVVEADAIYVKPFETETILIAPGQTTNV 270

Query: 243 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEYHHQQQ----QSTTGF----V 302
           LL TK +   + +F+M ARP+ T Q  TFDNSTV GILEY   +Q     S T      +
Sbjct: 271 LLKTK-SSYPSASFFMTARPYVTGQ-GTFDNSTVAGILEYEPPKQTKGAHSRTSIKNLQL 330

Query: 303 YKPTLPAINDTAFAANFTNKIRSLTSFSFPK----KVDKRFFFTVGLGTSPCKRGPNRAC 362
           +KP LPA+NDT FA  F+NK+RSL S +FP      VD++FFFTVGLGT+PC    N+ C
Sbjct: 331 FKPILPALNDTNFATKFSNKLRSLNSKNFPANVPLNVDRKFFFTVGLGTNPCNHKNNQTC 390

Query: 363 QGP-NGSRFAASINNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNT 422
           QGP N + FAASI+N+SF++P  ALLQ+HY G+++G+Y+  FP  P   FN+TG P NNT
Sbjct: 391 QGPTNTTMFAASISNISFTMPTKALLQSHYSGQSHGVYSPKFPWSPIVPFNYTGTPPNNT 450

Query: 423 MVRNGTR--------------AGTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNF 478
           MV NGT                 TS++ AE+HP HLHG + FVVGQGFGNFDP KDP+NF
Sbjct: 451 MVSNGTNLMVLPYNTSVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPNKDPRNF 510

BLAST of Bhi10M000508 vs. ExPASy Swiss-Prot
Match: O81081 (Laccase-2 OS=Arabidopsis thaliana OX=3702 GN=LAC2 PE=2 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 3.5e-173
Identity = 302/544 (55.51%), Postives = 368/544 (67.65%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           IQ++++TRLC +K I TVNGKFPGP +   EGD L +KVVN + NN++IHWHGIRQ R+G
Sbjct: 36  IQLKNITRLCKTKTIVTVNGKFPGPRVTAREGDNLQIKVVNHVSNNISIHWHGIRQLRSG 95

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGP+Y+TQCPI+ GQSY Y FT+ GQRGTLWWH HI W+RATVYGPLIILP  L   Y
Sbjct: 96  WADGPSYVTQCPIRMGQSYVYNFTVTGQRGTLWWHAHIQWMRATVYGPLIILP-KLHQPY 155

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PFP P+K+V P++FGEW+N D QAV+ +ALQ G  PN SDA T NGLPGP YNCS     
Sbjct: 156 PFPKPYKQV-PILFGEWFNADPQAVVQQALQTGAGPNASDAHTFNGLPGPLYNCSTKDTY 215

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            VVEADA YVKPFQTNI+L+ PGQTTNV
Sbjct: 216 KLMVKPGKTYLLRLINAALNDELFFTIANHTLTVVEADACYVKPFQTNIVLLGPGQTTNV 275

Query: 243 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEYHHQQQQSTTGFVYKPTLPAI 302
           LL TKP    N TFYMLARP+ T Q  T DN+TV GIL+Y H  + S    + KP+LP I
Sbjct: 276 LLKTKP-IYPNATFYMLARPYFTGQ-GTIDNTTVAGILQYQHHTKSSKNLSIIKPSLPPI 335

Query: 303 NDTAFAANFTNKIRSLTSFSF----PKKVDKRFFFTVGLGTSPCKRGPNRACQGP-NGSR 362
           N T++AANFT   RSL S +F    PK VDK++FF +GLGT+PC +  N+ CQGP N ++
Sbjct: 336 NSTSYAANFTKMFRSLASSTFPANVPKVVDKQYFFAIGLGTNPCPK--NQTCQGPTNTTK 395

Query: 363 FAASINNVSFSLPN-TALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRNGTR 422
           FAASINNVSF LPN T+LLQ++++G++  ++  DFP  P   FN+TG P NNTMV  GT+
Sbjct: 396 FAASINNVSFILPNKTSLLQSYFVGKSKNVFMTDFPTAPIIPFNYTGTPPNNTMVSRGTK 455

Query: 423 A--------------GTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVDPMR 478
                          GTS++  E HP HLHG + +VVGQGFGNF+P +DP+++NLVDP+ 
Sbjct: 456 VVVLKYKTTVELVLQGTSILGIEAHPIHLHGFNFYVVGQGFGNFNPARDPKHYNLVDPVE 515

BLAST of Bhi10M000508 vs. ExPASy Swiss-Prot
Match: Q10ND7 (Laccase-10 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC10 PE=2 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 5.1e-172
Identity = 298/545 (54.68%), Postives = 371/545 (68.07%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           +++Q+VTRLC+++ I TVNGKFPGP I+  EGD +VVKVVN I++N+TIHWHG+RQ RTG
Sbjct: 38  VKLQNVTRLCNTRAIPTVNGKFPGPKIVTREGDRVVVKVVNNIKDNITIHWHGVRQMRTG 97

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPN-NLPLA 122
           W+DGPAY+TQCPIQ GQSY Y FTI GQRGTL+WH H+SWLR+T+YGP+IILP   LPL 
Sbjct: 98  WSDGPAYVTQCPIQTGQSYVYNFTINGQRGTLFWHAHVSWLRSTLYGPIIILPKAGLPL- 157

Query: 123 YPFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH-- 182
            PF  P K+V P++FGEW+N D +A++ +ALQ GG PN+SDA+TINGLPGP YNCS+   
Sbjct: 158 -PFTEPHKDV-PIIFGEWFNADPEAIVAQALQTGGGPNVSDAYTINGLPGPLYNCSSKDT 217

Query: 183 ----------------------------------VVEADANYVKPFQTNIILIAPGQTTN 242
                                             VV+ DA+YVKPF T+++LI PGQTTN
Sbjct: 218 FRLKVQPGKMYLLRLINAALNDELFFSVANHTLTVVDVDASYVKPFDTDVVLITPGQTTN 277

Query: 243 VLLHTKPNPQ-NNDTFYMLARPFSTAQPATFDNSTVGGILEYHHQQQQSTTGFVYKPTLP 302
           VLL  KP  +    T  M+ARP++T +P T+DN+TV  +LEY       +   + +P+LP
Sbjct: 278 VLLRAKPTAEAAGATHLMMARPYATGRPGTYDNTTVAAVLEYAPPGHIKSLPLL-RPSLP 337

Query: 303 AINDTAFAANFTNKIRSLTSFSF----PKKVDKRFFFTVGLGTSPCKRGPNRACQGP-NG 362
           A+NDTAFAA F  K+RSL    +    P++VDK FFF VGLGT+PC    N+ CQGP N 
Sbjct: 338 ALNDTAFAAGFAAKLRSLACPDYPSNVPRRVDKPFFFAVGLGTTPCPGSNNQTCQGPTNT 397

Query: 363 SRFAASINNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRNGT 422
           ++F ASINNVSF +P TALLQAHY G++ G+YT DFP  P   FN+TG P NNT V NGT
Sbjct: 398 TKFTASINNVSFDMPTTALLQAHYTGQSAGVYTADFPASPLEPFNYTGTPPNNTNVSNGT 457

Query: 423 RA--------------GTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVDPM 478
           R                TS++ AE+HP HLHG D FVVGQG GN+DP K P  FNLVDP+
Sbjct: 458 RVVVLPYNASVEVVLQDTSILGAESHPLHLHGFDFFVVGQGTGNYDPSKHPAEFNLVDPV 517

BLAST of Bhi10M000508 vs. ExPASy Swiss-Prot
Match: Q5N9X2 (Laccase-4 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC4 PE=2 SV=1)

HSP 1 Score: 600.1 bits (1546), Expect = 2.1e-170
Identity = 299/549 (54.46%), Postives = 364/549 (66.30%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           +QM + TRLC++K++ TVNG+ PGP ++  EGD +V++V N + +N+++HWHG+RQ RTG
Sbjct: 37  VQMANATRLCNTKSMVTVNGQCPGPELVAREGDRVVIRVTNNVAHNISLHWHGVRQVRTG 96

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGPAYITQCPIQ GQSY Y FT+ GQRGTLWWH HISWLRATVYG L+ILP  L + Y
Sbjct: 97  WADGPAYITQCPIQTGQSYVYNFTVAGQRGTLWWHAHISWLRATVYGALVILP-KLGVPY 156

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PFP P KEV P++FGEWWN DT+ V+ +A+Q GG PN+SDAFTINGLPGP YNCSA    
Sbjct: 157 PFPAPHKEV-PVIFGEWWNADTEEVVNQAVQTGGGPNVSDAFTINGLPGPLYNCSAQDTF 216

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            VVE DA YVKPF  + ++I+PGQTTNV
Sbjct: 217 KLKVKPGKTYMLRLINAALNEELFFAVANHTLTVVEVDAVYVKPFTVDTLVISPGQTTNV 276

Query: 243 LLHTKP-NPQNNDTFYMLARPFSTAQPATFDNSTVGGILEYHH-QQQQSTTGFV-----Y 302
           LL  KP  P  N  FYM A P+STA+P TF N+TV GILEY +     S   FV     +
Sbjct: 277 LLTAKPYYPGAN--FYMSAAPYSTARPGTFGNTTVAGILEYENPAMSPSAASFVKGLPLF 336

Query: 303 KPTLPAINDTAFAANFTNKIRSLTSFSF----PKKVDKRFFFTVGLGTSPCKRGPNRACQ 362
           KPTLP +NDT F  NFT+K+RSL +  +    P+ VDKRFFFTVGLGT PC    N  CQ
Sbjct: 337 KPTLPQLNDTDFVTNFTDKLRSLATPEYPAAVPQSVDKRFFFTVGLGTLPCP--ANMTCQ 396

Query: 363 GPNGSRFAASINNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMV 422
           GPN ++ AAS+NNVSF LP  ALLQ+H+ G ++G+Y  DFP  P   FN+TG P NNT V
Sbjct: 397 GPNNTQMAASMNNVSFVLPARALLQSHFTGLSSGVYAPDFPVAPLSPFNYTGTPPNNTNV 456

Query: 423 RNGTR--------------AGTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNL 478
           + GT+                TS++  E+HP HLHG + FV+GQGFGN+D   DP  FNL
Sbjct: 457 KTGTKLLVLRYNTSVELVMQDTSILGIESHPLHLHGFNFFVIGQGFGNYDAVNDPAKFNL 516

BLAST of Bhi10M000508 vs. ExPASy Swiss-Prot
Match: B9FJH4 (Laccase-12 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC12 PE=3 SV=2)

HSP 1 Score: 599.7 bits (1545), Expect = 2.8e-170
Identity = 303/545 (55.60%), Postives = 364/545 (66.79%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           +Q  SVTRLC +K+I TVNG++PGP++   EGD + V VVN    N++IHWHGIRQ  +G
Sbjct: 36  VQTTSVTRLCSTKSIVTVNGQYPGPTLFAREGDHVEVTVVNHSPYNMSIHWHGIRQLLSG 95

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGP+YITQCPIQPG SY Y+FTI GQRGTLWWH HISWLRATV+GP++ILP    + Y
Sbjct: 96  WADGPSYITQCPIQPGGSYVYRFTITGQRGTLWWHAHISWLRATVHGPMVILP-PAGVGY 155

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PFP P +EV P+MFGEWWN+DT+AVI++ALQ GG PNISDA+T+NGLPGP YNCSA    
Sbjct: 156 PFPAPHEEV-PIMFGEWWNNDTEAVISQALQTGGGPNISDAYTLNGLPGPLYNCSAQDTF 215

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            VV+ DA YVKPF  + ++IAPGQT+NV
Sbjct: 216 KLKVKPGKTYMLRLINAALNDELFFSIANHTLTVVDVDALYVKPFTVDTLIIAPGQTSNV 275

Query: 243 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEYHHQQQQSTTGF---VYKPTL 302
           LL  KP      ++YMLARP++T Q  TFDN+TV G+LEY      +  G    ++ PTL
Sbjct: 276 LLTAKPT-YPGASYYMLARPYTTTQ-GTFDNTTVAGVLEYDDPCPTTAAGKIVPIFSPTL 335

Query: 303 PAINDTAFAANFTNKIRSLTSFSF----PKKVDKRFFFTVGLGTSPCKRGPNRACQGPNG 362
           P INDT   +NFT K+RSL S  +    P++VD RFFFTVGLGT PC    N  CQGPNG
Sbjct: 336 PQINDTNAVSNFTAKLRSLASAGYPAAVPQQVDHRFFFTVGLGTHPC--AVNGTCQGPNG 395

Query: 363 SRFAASINNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRNGT 422
           SRFAASINNVSF LP TALLQ+H+ G++ G+Y  +FP  P   FN+TG P NNT V NGT
Sbjct: 396 SRFAASINNVSFVLPATALLQSHFAGKSKGVYASNFPYYPLNPFNYTGTPPNNTNVMNGT 455

Query: 423 RA--------------GTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVDPM 478
           +                TS++ AE+HP HLHG + FVVGQGFGNFDP  DP  FNL DP+
Sbjct: 456 KVLVLPYGANVELVMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPINDPAKFNLYDPV 515

BLAST of Bhi10M000508 vs. NCBI nr
Match: XP_038902160.1 (laccase-17-like [Benincasa hispida])

HSP 1 Score: 981.5 bits (2536), Expect = 2.6e-282
Identity = 475/538 (88.29%), Postives = 475/538 (88.29%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG
Sbjct: 39  IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 98

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY
Sbjct: 99  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 158

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH   
Sbjct: 159 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAHDTY 218

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            VVEADANYVKPFQTNIILIAPGQTTNV
Sbjct: 219 KLKVEAGKTYLLRIINAALNDDLFFTIANHTLTVVEADANYVKPFQTNIILIAPGQTTNV 278

Query: 243 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEYHHQQQQSTTGFVYKPTLPAI 302
           LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEYHHQQQQSTTGFVYKPTLPAI
Sbjct: 279 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEYHHQQQQSTTGFVYKPTLPAI 338

Query: 303 NDTAFAANFTNKIRSLTSFSFPKKVDKRFFFTVGLGTSPCKRGPNRACQGPNGSRFAASI 362
           NDTAFAANFTNKIRSLTSFSFPKKVDKRFFFTVGLGTSPCKRGPNRACQGPNGSRFAASI
Sbjct: 339 NDTAFAANFTNKIRSLTSFSFPKKVDKRFFFTVGLGTSPCKRGPNRACQGPNGSRFAASI 398

Query: 363 NNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRNGTRA----- 422
           NNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRNGTRA     
Sbjct: 399 NNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRNGTRAVWLDF 458

Query: 423 ---------GTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVDPMRRNTFGV 478
                    GTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVDPMRRNTFGV
Sbjct: 459 NTSVELILQGTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVDPMRRNTFGV 518

BLAST of Bhi10M000508 vs. NCBI nr
Match: XP_004148786.1 (laccase-17 [Cucumis sativus] >KGN64741.1 hypothetical protein Csa_013745 [Cucumis sativus])

HSP 1 Score: 663.3 bits (1710), Expect = 1.6e-186
Identity = 324/545 (59.45%), Postives = 390/545 (71.56%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           I+MQ+VTRLCH+K+I TVNGKFPGP I+  EGD L++KVVN + NN++IHWHGIRQ R+G
Sbjct: 39  IKMQNVTRLCHTKSIVTVNGKFPGPRIVAREGDRLLIKVVNNVPNNISIHWHGIRQLRSG 98

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGPAYITQCPIQ GQSY Y +TIIGQRGTL+WH HISWLR+T+YGP+IILP  L + Y
Sbjct: 99  WADGPAYITQCPIQSGQSYVYNYTIIGQRGTLFWHAHISWLRSTLYGPIIILP-KLGVPY 158

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PFP P KE+ P++FGEWWN DT+AVI++ALQ GG PN+SDA+T+NGLPGPFYNCS     
Sbjct: 159 PFPKPHKEM-PIIFGEWWNVDTEAVISQALQTGGGPNVSDAYTMNGLPGPFYNCSTKDTL 218

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            +V+ DA Y+KPF+TN ++IAPGQT+NV
Sbjct: 219 KLKVKPGKTYLLRLINAALNDELFFSIANHSLTIVDVDAVYIKPFKTNTLIIAPGQTSNV 278

Query: 243 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEY--HHQQQQSTTGFVYKPTLP 302
           LL TK N   N  FYMLARP+ T Q  TFDNSTV GILEY  H Q+    T  +YKP LP
Sbjct: 279 LLQTKSN-FPNAKFYMLARPYVTGQ-GTFDNSTVAGILEYDDHQQKNPKKTLPIYKPNLP 338

Query: 303 AINDTAFAANFTNKIRSLTSFSF----PKKVDKRFFFTVGLGTSPCKRG-PNRACQGPNG 362
           ++NDT+F  NFT K+RSL +  F    P+K+DK+FFFTVGLGT+PC     N+ CQGPNG
Sbjct: 339 SLNDTSFVTNFTKKLRSLANSQFPANVPQKIDKKFFFTVGLGTNPCSNNKKNQTCQGPNG 398

Query: 363 SRFAASINNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRNGT 422
           + FAASINNVSF +PN ALLQ+HY G++NG+Y+  FPN P   FN+TG P NNTMV NGT
Sbjct: 399 TMFAASINNVSFIMPNVALLQSHYKGQSNGVYSPFFPNSPLIPFNYTGNPPNNTMVGNGT 458

Query: 423 RA--------------GTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVDPM 478
           +                TS++ AE+HP HLHG + FVVGQGFGNFDP KDPQNFNLVDP+
Sbjct: 459 KVVVLPFNTSVELIMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPNKDPQNFNLVDPV 518

BLAST of Bhi10M000508 vs. NCBI nr
Match: XP_008463808.1 (PREDICTED: LOW QUALITY PROTEIN: laccase-17-like [Cucumis melo])

HSP 1 Score: 662.5 bits (1708), Expect = 2.7e-186
Identity = 324/545 (59.45%), Postives = 390/545 (71.56%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           I+MQ+VTRLCH+K+I TVNGKFPGP I+  EGD L++KVVN + NN++IHWHGIRQ R+G
Sbjct: 38  IKMQNVTRLCHTKSIVTVNGKFPGPRIVAREGDRLLIKVVNHVPNNISIHWHGIRQLRSG 97

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGPAYITQCPIQ GQSY Y +TIIGQRGTL+WH HISWLR+T+YGP+IILP  L + Y
Sbjct: 98  WADGPAYITQCPIQSGQSYVYNYTIIGQRGTLFWHAHISWLRSTLYGPIIILP-KLGVPY 157

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PFP P KE+ P++FGEWWN DT+AVI++ALQ GG PN+SDA+T+NGLPGPFYNCSA    
Sbjct: 158 PFPKPHKEM-PIIFGEWWNVDTEAVISQALQTGGGPNVSDAYTMNGLPGPFYNCSAKDTL 217

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            +V+ DA Y+KP +TN ++IAPGQT+NV
Sbjct: 218 KLKVKPGKTYLLRLINAALNDELFFSIANHSLTIVDVDAVYIKPLKTNTLIIAPGQTSNV 277

Query: 243 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEY--HHQQQQSTTGFVYKPTLP 302
           LL TK N   N  FYMLARP+ T Q  TFDNSTV GILEY  H Q+    T  +YKP LP
Sbjct: 278 LLQTKSN-FPNAKFYMLARPYVTGQ-GTFDNSTVAGILEYDGHQQKNPKKTLPIYKPNLP 337

Query: 303 AINDTAFAANFTNKIRSLTSFSF----PKKVDKRFFFTVGLGTSPCKRG-PNRACQGPNG 362
           ++NDT+F  NFT K+RSL +  F    P+K+DK+FFFTVGLGT+PC     N+ CQGPNG
Sbjct: 338 SLNDTSFVTNFTKKLRSLANSQFPANVPQKIDKKFFFTVGLGTNPCNNNKKNQTCQGPNG 397

Query: 363 SRFAASINNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRNGT 422
           + FAASINNVSF +PN ALLQ+HY G++NG+Y+  FPN P   FN+TG P NNTMV NGT
Sbjct: 398 TMFAASINNVSFVMPNVALLQSHYKGQSNGVYSPFFPNSPLIPFNYTGNPPNNTMVGNGT 457

Query: 423 RA--------------GTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVDPM 478
           +                TS++ AE+HP HLHG + FVVGQGFGNFDP KDPQNFNLVDP+
Sbjct: 458 KVVVLPFNTSVELIMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPNKDPQNFNLVDPV 517

BLAST of Bhi10M000508 vs. NCBI nr
Match: KAA0056762.1 (laccase-17-like [Cucumis melo var. makuwa])

HSP 1 Score: 662.5 bits (1708), Expect = 2.7e-186
Identity = 324/545 (59.45%), Postives = 390/545 (71.56%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           I+MQ+VTRLCH+K+I TVNGKFPGP I+  EGD L++KVVN + NN++IHWHGIRQ R+G
Sbjct: 26  IKMQNVTRLCHTKSIVTVNGKFPGPRIVAREGDRLLIKVVNHVPNNISIHWHGIRQLRSG 85

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGPAYITQCPIQ GQSY Y +TIIGQRGTL+WH HISWLR+T+YGP+IILP  L + Y
Sbjct: 86  WADGPAYITQCPIQSGQSYVYNYTIIGQRGTLFWHAHISWLRSTLYGPIIILP-KLGVPY 145

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PFP P KE+ P++FGEWWN DT+AVI++ALQ GG PN+SDA+T+NGLPGPFYNCSA    
Sbjct: 146 PFPKPHKEM-PIIFGEWWNVDTEAVISQALQTGGGPNVSDAYTMNGLPGPFYNCSAKDTL 205

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            +V+ DA Y+KP +TN ++IAPGQT+NV
Sbjct: 206 KLKVKPGKTYLLRLINAALNDELFFSIANHSLTIVDVDAVYIKPLKTNTLIIAPGQTSNV 265

Query: 243 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEY--HHQQQQSTTGFVYKPTLP 302
           LL TK N   N  FYMLARP+ T Q  TFDNSTV GILEY  H Q+    T  +YKP LP
Sbjct: 266 LLQTKSN-FPNAKFYMLARPYVTGQ-GTFDNSTVAGILEYDGHQQKNPKKTLPIYKPNLP 325

Query: 303 AINDTAFAANFTNKIRSLTSFSF----PKKVDKRFFFTVGLGTSPCKRG-PNRACQGPNG 362
           ++NDT+F  NFT K+RSL +  F    P+K+DK+FFFTVGLGT+PC     N+ CQGPNG
Sbjct: 326 SLNDTSFVTNFTKKLRSLANSQFPANVPQKIDKKFFFTVGLGTNPCNNNKKNQTCQGPNG 385

Query: 363 SRFAASINNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRNGT 422
           + FAASINNVSF +PN ALLQ+HY G++NG+Y+  FPN P   FN+TG P NNTMV NGT
Sbjct: 386 TMFAASINNVSFVMPNVALLQSHYKGQSNGVYSPFFPNSPLIPFNYTGNPPNNTMVGNGT 445

Query: 423 RA--------------GTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVDPM 478
           +                TS++ AE+HP HLHG + FVVGQGFGNFDP KDPQNFNLVDP+
Sbjct: 446 KVVVLPFNTSVELIMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPNKDPQNFNLVDPV 505

BLAST of Bhi10M000508 vs. NCBI nr
Match: RWR95403.1 (laccase-17 [Cinnamomum micranthum f. kanehirae])

HSP 1 Score: 661.8 bits (1706), Expect = 4.6e-186
Identity = 325/547 (59.41%), Postives = 384/547 (70.20%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           I++Q+VTRLCHS++I TVNG+FPGP I+  EGD +VVKVVN +QNNVTIHWHG+RQ R+G
Sbjct: 45  IRLQNVTRLCHSRSIVTVNGQFPGPRIVAREGDRVVVKVVNHVQNNVTIHWHGVRQLRSG 104

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGPAY+TQCPIQ GQSY Y FTI GQRGTL+WH HISWLRATVYGPLIILP    + Y
Sbjct: 105 WADGPAYVTQCPIQTGQSYIYNFTITGQRGTLFWHAHISWLRATVYGPLIILPKQ-GVPY 164

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PF  P+KEV P++FGEW+N DT+A+I++ALQ GG PN+SDA+TINGLPGP YNCSA    
Sbjct: 165 PFARPYKEV-PIIFGEWFNTDTEAIISQALQTGGGPNVSDAYTINGLPGPLYNCSAKDTF 224

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            VVEADA Y KPFQ   ILI PGQTTNV
Sbjct: 225 KLNVKPGKTYLLRLINAALNDELFFSIANHTLTVVEADATYTKPFQAETILITPGQTTNV 284

Query: 243 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEYHHQQQQSTTGF-----VYKP 302
           LL TKP+   N +F M ARP+ T  P TFDNST  GILEY      S++       +++P
Sbjct: 285 LLKTKPH-LPNASFLMAARPYVTG-PGTFDNSTTAGILEYQRNNSSSSSSSTTNLPLFRP 344

Query: 303 TLPAINDTAFAANFTNKIRSLTSFSF----PKKVDKRFFFTVGLGTSPCKRGPNRACQGP 362
           TLP +NDT+FAANFT + RSL +  F    P+KVDKRFFFTVGLGTSPC    N+ CQGP
Sbjct: 345 TLPPLNDTSFAANFTKRFRSLANAKFPANVPQKVDKRFFFTVGLGTSPCPN--NQTCQGP 404

Query: 363 NGSRFAASINNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRN 422
           NG++FAAS+NN+SF  P TALLQAH+ G++NG+YT DFPN P + FN+TG P NNTMV N
Sbjct: 405 NGTKFAASVNNISFIQPTTALLQAHFFGQSNGVYTTDFPNNPPFPFNYTGTPPNNTMVSN 464

Query: 423 GTR--------------AGTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVD 478
           GT+                TS++ AE+HP HLHG + +VVGQGFGN+DPKKDP  FNL+D
Sbjct: 465 GTKLVVLPFNTSVELVMQDTSIIGAESHPLHLHGFNFYVVGQGFGNYDPKKDPSKFNLID 524

BLAST of Bhi10M000508 vs. ExPASy TrEMBL
Match: A0A0A0LXL0 (Laccase OS=Cucumis sativus OX=3659 GN=Csa_1G085370 PE=3 SV=1)

HSP 1 Score: 663.3 bits (1710), Expect = 7.6e-187
Identity = 324/545 (59.45%), Postives = 390/545 (71.56%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           I+MQ+VTRLCH+K+I TVNGKFPGP I+  EGD L++KVVN + NN++IHWHGIRQ R+G
Sbjct: 39  IKMQNVTRLCHTKSIVTVNGKFPGPRIVAREGDRLLIKVVNNVPNNISIHWHGIRQLRSG 98

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGPAYITQCPIQ GQSY Y +TIIGQRGTL+WH HISWLR+T+YGP+IILP  L + Y
Sbjct: 99  WADGPAYITQCPIQSGQSYVYNYTIIGQRGTLFWHAHISWLRSTLYGPIIILP-KLGVPY 158

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PFP P KE+ P++FGEWWN DT+AVI++ALQ GG PN+SDA+T+NGLPGPFYNCS     
Sbjct: 159 PFPKPHKEM-PIIFGEWWNVDTEAVISQALQTGGGPNVSDAYTMNGLPGPFYNCSTKDTL 218

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            +V+ DA Y+KPF+TN ++IAPGQT+NV
Sbjct: 219 KLKVKPGKTYLLRLINAALNDELFFSIANHSLTIVDVDAVYIKPFKTNTLIIAPGQTSNV 278

Query: 243 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEY--HHQQQQSTTGFVYKPTLP 302
           LL TK N   N  FYMLARP+ T Q  TFDNSTV GILEY  H Q+    T  +YKP LP
Sbjct: 279 LLQTKSN-FPNAKFYMLARPYVTGQ-GTFDNSTVAGILEYDDHQQKNPKKTLPIYKPNLP 338

Query: 303 AINDTAFAANFTNKIRSLTSFSF----PKKVDKRFFFTVGLGTSPCKRG-PNRACQGPNG 362
           ++NDT+F  NFT K+RSL +  F    P+K+DK+FFFTVGLGT+PC     N+ CQGPNG
Sbjct: 339 SLNDTSFVTNFTKKLRSLANSQFPANVPQKIDKKFFFTVGLGTNPCSNNKKNQTCQGPNG 398

Query: 363 SRFAASINNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRNGT 422
           + FAASINNVSF +PN ALLQ+HY G++NG+Y+  FPN P   FN+TG P NNTMV NGT
Sbjct: 399 TMFAASINNVSFIMPNVALLQSHYKGQSNGVYSPFFPNSPLIPFNYTGNPPNNTMVGNGT 458

Query: 423 RA--------------GTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVDPM 478
           +                TS++ AE+HP HLHG + FVVGQGFGNFDP KDPQNFNLVDP+
Sbjct: 459 KVVVLPFNTSVELIMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPNKDPQNFNLVDPV 518

BLAST of Bhi10M000508 vs. ExPASy TrEMBL
Match: A0A5A7ULP7 (Laccase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold486G00400 PE=3 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 1.3e-186
Identity = 324/545 (59.45%), Postives = 390/545 (71.56%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           I+MQ+VTRLCH+K+I TVNGKFPGP I+  EGD L++KVVN + NN++IHWHGIRQ R+G
Sbjct: 26  IKMQNVTRLCHTKSIVTVNGKFPGPRIVAREGDRLLIKVVNHVPNNISIHWHGIRQLRSG 85

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGPAYITQCPIQ GQSY Y +TIIGQRGTL+WH HISWLR+T+YGP+IILP  L + Y
Sbjct: 86  WADGPAYITQCPIQSGQSYVYNYTIIGQRGTLFWHAHISWLRSTLYGPIIILP-KLGVPY 145

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PFP P KE+ P++FGEWWN DT+AVI++ALQ GG PN+SDA+T+NGLPGPFYNCSA    
Sbjct: 146 PFPKPHKEM-PIIFGEWWNVDTEAVISQALQTGGGPNVSDAYTMNGLPGPFYNCSAKDTL 205

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            +V+ DA Y+KP +TN ++IAPGQT+NV
Sbjct: 206 KLKVKPGKTYLLRLINAALNDELFFSIANHSLTIVDVDAVYIKPLKTNTLIIAPGQTSNV 265

Query: 243 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEY--HHQQQQSTTGFVYKPTLP 302
           LL TK N   N  FYMLARP+ T Q  TFDNSTV GILEY  H Q+    T  +YKP LP
Sbjct: 266 LLQTKSN-FPNAKFYMLARPYVTGQ-GTFDNSTVAGILEYDGHQQKNPKKTLPIYKPNLP 325

Query: 303 AINDTAFAANFTNKIRSLTSFSF----PKKVDKRFFFTVGLGTSPCKRG-PNRACQGPNG 362
           ++NDT+F  NFT K+RSL +  F    P+K+DK+FFFTVGLGT+PC     N+ CQGPNG
Sbjct: 326 SLNDTSFVTNFTKKLRSLANSQFPANVPQKIDKKFFFTVGLGTNPCNNNKKNQTCQGPNG 385

Query: 363 SRFAASINNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRNGT 422
           + FAASINNVSF +PN ALLQ+HY G++NG+Y+  FPN P   FN+TG P NNTMV NGT
Sbjct: 386 TMFAASINNVSFVMPNVALLQSHYKGQSNGVYSPFFPNSPLIPFNYTGNPPNNTMVGNGT 445

Query: 423 RA--------------GTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVDPM 478
           +                TS++ AE+HP HLHG + FVVGQGFGNFDP KDPQNFNLVDP+
Sbjct: 446 KVVVLPFNTSVELIMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPNKDPQNFNLVDPV 505

BLAST of Bhi10M000508 vs. ExPASy TrEMBL
Match: A0A1S3CK53 (Laccase OS=Cucumis melo OX=3656 GN=LOC103501858 PE=3 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 1.3e-186
Identity = 324/545 (59.45%), Postives = 390/545 (71.56%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           I+MQ+VTRLCH+K+I TVNGKFPGP I+  EGD L++KVVN + NN++IHWHGIRQ R+G
Sbjct: 38  IKMQNVTRLCHTKSIVTVNGKFPGPRIVAREGDRLLIKVVNHVPNNISIHWHGIRQLRSG 97

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGPAYITQCPIQ GQSY Y +TIIGQRGTL+WH HISWLR+T+YGP+IILP  L + Y
Sbjct: 98  WADGPAYITQCPIQSGQSYVYNYTIIGQRGTLFWHAHISWLRSTLYGPIIILP-KLGVPY 157

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PFP P KE+ P++FGEWWN DT+AVI++ALQ GG PN+SDA+T+NGLPGPFYNCSA    
Sbjct: 158 PFPKPHKEM-PIIFGEWWNVDTEAVISQALQTGGGPNVSDAYTMNGLPGPFYNCSAKDTL 217

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            +V+ DA Y+KP +TN ++IAPGQT+NV
Sbjct: 218 KLKVKPGKTYLLRLINAALNDELFFSIANHSLTIVDVDAVYIKPLKTNTLIIAPGQTSNV 277

Query: 243 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEY--HHQQQQSTTGFVYKPTLP 302
           LL TK N   N  FYMLARP+ T Q  TFDNSTV GILEY  H Q+    T  +YKP LP
Sbjct: 278 LLQTKSN-FPNAKFYMLARPYVTGQ-GTFDNSTVAGILEYDGHQQKNPKKTLPIYKPNLP 337

Query: 303 AINDTAFAANFTNKIRSLTSFSF----PKKVDKRFFFTVGLGTSPCKRG-PNRACQGPNG 362
           ++NDT+F  NFT K+RSL +  F    P+K+DK+FFFTVGLGT+PC     N+ CQGPNG
Sbjct: 338 SLNDTSFVTNFTKKLRSLANSQFPANVPQKIDKKFFFTVGLGTNPCNNNKKNQTCQGPNG 397

Query: 363 SRFAASINNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRNGT 422
           + FAASINNVSF +PN ALLQ+HY G++NG+Y+  FPN P   FN+TG P NNTMV NGT
Sbjct: 398 TMFAASINNVSFVMPNVALLQSHYKGQSNGVYSPFFPNSPLIPFNYTGNPPNNTMVGNGT 457

Query: 423 RA--------------GTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVDPM 478
           +                TS++ AE+HP HLHG + FVVGQGFGNFDP KDPQNFNLVDP+
Sbjct: 458 KVVVLPFNTSVELIMQDTSILGAESHPLHLHGFNFFVVGQGFGNFDPNKDPQNFNLVDPV 517

BLAST of Bhi10M000508 vs. ExPASy TrEMBL
Match: A0A3S3R4V1 (Laccase OS=Cinnamomum micranthum f. kanehirae OX=337451 GN=CKAN_02474300 PE=3 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 2.2e-186
Identity = 325/547 (59.41%), Postives = 384/547 (70.20%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           I++Q+VTRLCHS++I TVNG+FPGP I+  EGD +VVKVVN +QNNVTIHWHG+RQ R+G
Sbjct: 45  IRLQNVTRLCHSRSIVTVNGQFPGPRIVAREGDRVVVKVVNHVQNNVTIHWHGVRQLRSG 104

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGPAY+TQCPIQ GQSY Y FTI GQRGTL+WH HISWLRATVYGPLIILP    + Y
Sbjct: 105 WADGPAYVTQCPIQTGQSYIYNFTITGQRGTLFWHAHISWLRATVYGPLIILPKQ-GVPY 164

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PF  P+KEV P++FGEW+N DT+A+I++ALQ GG PN+SDA+TINGLPGP YNCSA    
Sbjct: 165 PFARPYKEV-PIIFGEWFNTDTEAIISQALQTGGGPNVSDAYTINGLPGPLYNCSAKDTF 224

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            VVEADA Y KPFQ   ILI PGQTTNV
Sbjct: 225 KLNVKPGKTYLLRLINAALNDELFFSIANHTLTVVEADATYTKPFQAETILITPGQTTNV 284

Query: 243 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEYHHQQQQSTTGF-----VYKP 302
           LL TKP+   N +F M ARP+ T  P TFDNST  GILEY      S++       +++P
Sbjct: 285 LLKTKPH-LPNASFLMAARPYVTG-PGTFDNSTTAGILEYQRNNSSSSSSSTTNLPLFRP 344

Query: 303 TLPAINDTAFAANFTNKIRSLTSFSF----PKKVDKRFFFTVGLGTSPCKRGPNRACQGP 362
           TLP +NDT+FAANFT + RSL +  F    P+KVDKRFFFTVGLGTSPC    N+ CQGP
Sbjct: 345 TLPPLNDTSFAANFTKRFRSLANAKFPANVPQKVDKRFFFTVGLGTSPCPN--NQTCQGP 404

Query: 363 NGSRFAASINNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRN 422
           NG++FAAS+NN+SF  P TALLQAH+ G++NG+YT DFPN P + FN+TG P NNTMV N
Sbjct: 405 NGTKFAASVNNISFIQPTTALLQAHFFGQSNGVYTTDFPNNPPFPFNYTGTPPNNTMVSN 464

Query: 423 GTR--------------AGTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVD 478
           GT+                TS++ AE+HP HLHG + +VVGQGFGN+DPKKDP  FNL+D
Sbjct: 465 GTKLVVLPFNTSVELVMQDTSIIGAESHPLHLHGFNFYVVGQGFGNYDPKKDPSKFNLID 524

BLAST of Bhi10M000508 vs. ExPASy TrEMBL
Match: A0A3S4PVM2 (Laccase OS=Cinnamomum micranthum f. kanehirae OX=337451 GN=CKAN_02473900 PE=3 SV=1)

HSP 1 Score: 661.4 bits (1705), Expect = 2.9e-186
Identity = 324/547 (59.23%), Postives = 384/547 (70.20%), Query Frame = 0

Query: 3   IQMQSVTRLCHSKNIATVNGKFPGPSIIVTEGDELVVKVVNRIQNNVTIHWHGIRQFRTG 62
           IQ+QSVTRLCHS++I TVNG+FPGP I+  EGD +VVKVVN++QNNVTIHWHG+RQ R G
Sbjct: 45  IQLQSVTRLCHSRSIVTVNGQFPGPRIVAREGDRVVVKVVNQVQNNVTIHWHGVRQLRNG 104

Query: 63  WADGPAYITQCPIQPGQSYNYKFTIIGQRGTLWWHGHISWLRATVYGPLIILPNNLPLAY 122
           WADGPAY+TQCPIQ GQSY Y FTI GQRGTL+WH HISW+RATVYGPLIILP    + Y
Sbjct: 105 WADGPAYVTQCPIQTGQSYIYNFTITGQRGTLFWHAHISWIRATVYGPLIILPKQ-GVPY 164

Query: 123 PFPNPFKEVHPLMFGEWWNDDTQAVITRALQNGGNPNISDAFTINGLPGPFYNCSAH--- 182
           PF  P+KEV P++FGEW+N DT+A+I++ALQ GG PN+SDA+TINGLPGP YNCSA    
Sbjct: 165 PFARPYKEV-PIIFGEWFNTDTEAIISQALQTGGGPNVSDAYTINGLPGPLYNCSAKDIF 224

Query: 183 ---------------------------------VVEADANYVKPFQTNIILIAPGQTTNV 242
                                            VVEADA Y+KPFQ   ILI PGQTTNV
Sbjct: 225 KLNVEPGKTYLLRLINAALNDELFFSIANHTLTVVEADATYIKPFQAETILITPGQTTNV 284

Query: 243 LLHTKPNPQNNDTFYMLARPFSTAQPATFDNSTVGGILEYHHQQQQSTTGF-----VYKP 302
           LL TKP+   N +F M ARP+ T  P TFDNST  GILEY      S++       +++P
Sbjct: 285 LLQTKPH-FPNASFLMAARPYVTG-PGTFDNSTTAGILEYQRNNSSSSSSSTTNLPLFRP 344

Query: 303 TLPAINDTAFAANFTNKIRSLTSFSF----PKKVDKRFFFTVGLGTSPCKRGPNRACQGP 362
           TLP +NDT+FAANFT + RSL +  F    P+KVDKRFFFTVGLGTSPC    N+ CQGP
Sbjct: 345 TLPPLNDTSFAANFTKRFRSLANAKFPANVPQKVDKRFFFTVGLGTSPCPN--NQTCQGP 404

Query: 363 NGSRFAASINNVSFSLPNTALLQAHYLGRANGIYTDDFPNGPEYFFNFTGPPLNNTMVRN 422
           NG++FAAS+NN+SF  P TALLQAH+ G++NG+YT DFPN P + FN+TG P NNT+V N
Sbjct: 405 NGTKFAASVNNISFIQPTTALLQAHFFGQSNGVYTTDFPNNPPFPFNYTGTPPNNTLVSN 464

Query: 423 GTR--------------AGTSLVNAENHPFHLHGHDVFVVGQGFGNFDPKKDPQNFNLVD 478
           GT+                TS++ AE+HP HLHG + +VVGQGFGN+DPK DP  FNL+D
Sbjct: 465 GTKLVVLPFNTSVELVMQDTSIIGAESHPLHLHGFNFYVVGQGFGNYDPKMDPSKFNLID 524

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G60020.14.9e-17856.62laccase 17 [more]
AT2G29130.12.5e-17455.51laccase 2 [more]
AT2G38080.15.8e-13947.50Laccase/Diphenol oxidase family protein [more]
AT5G03260.15.5e-13746.04laccase 11 [more]
AT5G05390.12.1e-13646.86laccase 12 [more]
Match NameE-valueIdentityDescription
Q9FJD56.9e-17756.62Laccase-17 OS=Arabidopsis thaliana OX=3702 GN=LAC17 PE=2 SV=1[more]
O810813.5e-17355.51Laccase-2 OS=Arabidopsis thaliana OX=3702 GN=LAC2 PE=2 SV=1[more]
Q10ND75.1e-17254.68Laccase-10 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC10 PE=2 SV=1[more]
Q5N9X22.1e-17054.46Laccase-4 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC4 PE=2 SV=1[more]
B9FJH42.8e-17055.60Laccase-12 OS=Oryza sativa subsp. japonica OX=39947 GN=LAC12 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
XP_038902160.12.6e-28288.29laccase-17-like [Benincasa hispida][more]
XP_004148786.11.6e-18659.45laccase-17 [Cucumis sativus] >KGN64741.1 hypothetical protein Csa_013745 [Cucumi... [more]
XP_008463808.12.7e-18659.45PREDICTED: LOW QUALITY PROTEIN: laccase-17-like [Cucumis melo][more]
KAA0056762.12.7e-18659.45laccase-17-like [Cucumis melo var. makuwa][more]
RWR95403.14.6e-18659.41laccase-17 [Cinnamomum micranthum f. kanehirae][more]
Match NameE-valueIdentityDescription
A0A0A0LXL07.6e-18759.45Laccase OS=Cucumis sativus OX=3659 GN=Csa_1G085370 PE=3 SV=1[more]
A0A5A7ULP71.3e-18659.45Laccase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold486G00400 PE=3 S... [more]
A0A1S3CK531.3e-18659.45Laccase OS=Cucumis melo OX=3656 GN=LOC103501858 PE=3 SV=1[more]
A0A3S3R4V12.2e-18659.41Laccase OS=Cinnamomum micranthum f. kanehirae OX=337451 GN=CKAN_02474300 PE=3 SV... [more]
A0A3S4PVM22.9e-18659.23Laccase OS=Cinnamomum micranthum f. kanehirae OX=337451 GN=CKAN_02473900 PE=3 SV... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR011707Multicopper oxidase, N-termianlPFAMPF07732Cu-oxidase_3coord: 3..116
e-value: 4.6E-42
score: 142.8
IPR001117Multicopper oxidase, type 1PFAMPF00394Cu-oxidasecoord: 180..248
e-value: 4.3E-12
score: 46.4
coord: 131..178
e-value: 9.2E-5
score: 22.6
IPR008972CupredoxinGENE3D2.60.40.420coord: 1..119
e-value: 3.2E-35
score: 122.9
IPR008972CupredoxinGENE3D2.60.40.420coord: 173..285
e-value: 4.0E-16
score: 61.7
IPR008972CupredoxinGENE3D2.60.40.420coord: 311..477
e-value: 1.1E-37
score: 131.7
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 287..477
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 125..282
IPR008972CupredoxinSUPERFAMILY49503Cupredoxinscoord: 3..141
IPR011706Multicopper oxidase, C-terminalPFAMPF07731Cu-oxidase_2coord: 387..448
e-value: 3.3E-22
score: 78.7
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 179..447
coord: 3..178
IPR045087Multicopper oxidasePANTHERPTHR11709MULTI-COPPER OXIDASEcoord: 449..477
NoneNo IPR availablePANTHERPTHR11709:SF402LACCASEcoord: 449..477
NoneNo IPR availablePANTHERPTHR11709:SF402LACCASEcoord: 3..178
NoneNo IPR availablePANTHERPTHR11709:SF402LACCASEcoord: 179..447
IPR034288Laccase, first cupredoxin domainCDDcd13849CuRO_1_LCC_plantcoord: 3..115
e-value: 3.98762E-70
score: 216.742

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Bhi10G000508Bhi10G000508gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi10M000508.exon7Bhi10M000508.exon7exon
Bhi10M000508.exon6Bhi10M000508.exon6exon
Bhi10M000508.exon5Bhi10M000508.exon5exon
Bhi10M000508.exon4Bhi10M000508.exon4exon
Bhi10M000508.exon3Bhi10M000508.exon3exon
Bhi10M000508.exon2Bhi10M000508.exon2exon
Bhi10M000508.exon1Bhi10M000508.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Bhi10M000508cds.Bhi10M000508_7CDS
cds.Bhi10M000508cds.Bhi10M000508_6CDS
cds.Bhi10M000508cds.Bhi10M000508_5CDS
cds.Bhi10M000508cds.Bhi10M000508_4CDS
cds.Bhi10M000508cds.Bhi10M000508_3CDS
cds.Bhi10M000508cds.Bhi10M000508_2CDS
cds.Bhi10M000508cds.Bhi10M000508CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Bhi10M000508Bhi10M000508-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005576 extracellular region
cellular_component GO:0016020 membrane
molecular_function GO:0005507 copper ion binding
molecular_function GO:0016491 oxidoreductase activity