Bhi09M000691 (mRNA) Wax gourd (B227) v1

Overview


Name	Bhi09M000691
Type	mRNA
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Glutathione transferase
Location	chr9: 18862875 .. 18865137 (+)
Sequence length	1423
RNA-Seq Expression	Bhi09M000691
Synteny	Bhi09M000691

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GATTTTGATCCATATACTAATTATGGGTTTCTTGAGAAAAGAAAGAAAAAGAACTTTATGAAACAGTTGATCAGCCGCGAATCTGAAATCGACCGCCATTAACGATCCGCGTTCTACGACCCCATCGATTTCCCATTTCGCTAAGAGATTCAGCAATGTCAAATTAATCCTTCCCTCATTCAATTCTCTATAAGAGAACCTTCAACAAATTCTTCTTCCCCACTCAAACCCATCTCAATTTCTTCGAATAATGGCGAGCATCAGAGTCCATGGAAGCCCCATTTCCACTGCCACAGCTAGAATTCTCGCTTGTCTTTACGAGAAAGATCTCGATTACGAGTTCGTTCTTCTGGACATGAAAAACAACGAGCACAAGAAACATCCCTTTCTCTCCATTAACGTCAGTATCTCTCTCTAATTGTGAATTTCTGAATTTTTTTCGATTGTTTTTTAATGAGTTTTTATGGAATTTCTGCAGCCGTTTGGTCAAGTTCCTGGGTTTCAAGATGGGGATCTCACCATTTTCGGTAATTAAGTGCTCTGTTTCGCTCTGAAAATGTTTTTTTGCACTCAACGTGTTTGATGAAATTCCCAAATATAGTTCCTACTTTATTATTGTTATTATTATTATTATTTTTTGGTAGAGATCTAGTTCCTACTCTATCAATAGGATTCAAGGTGATTTGATAAAACTTTTGGTTTAAATTATAAAAGATACCCACAATTGTTAAAAAAAAAACCCTTACTCTTTGAGTTCTTGACTTTCCAAATTCTTTTTAAATTTTGGATGGAAATTGGAATTTGAAATAGTATGTTGGCAACCTAGAATTGATATTTAGTTCGTCCTACCGTTTTAAGTTATTGTCACTCTTCTAGGTTAAAAAAGTCGATGATTATATTGTAATTTCCAAAAGAACCAAGGGTATTTGTGAAACAAAATGACGTTAAGGAAATTTTTGTTTGCAGTTTAGTCAAGTATGTTTAGATACTATTTTGATCATATACTTGTTCATTCTGGTTAGGTACTTTCAAAATGTTCATTTACGTTATCATAGACTCATGAAATAGTAATTAATGTCTTATTCAGTTAGCTAAGCTCAAATTGAGTGTAGAAATATATTGGGTATAGTGTTGTATTGAAACCGTAATTTGAAAATAAAATACAAAGACTAATATGGACAAAAGCTTTGCAAGCAATAGAAAATTTGTATTTAATCCAATAAAAGTTTCTATTCTGAAAATATAGATTGTGCTCAAGGTGTTGGATAAAAAAAATGTTTCCTGTGTAGAATCAAGGGCCATTACTCAATACTTGGCCAAGGCCTACGCCAGCAAGGGAACCCAATTGATCTCCGACGACCCAAAGAAAGCGGCGATCATATTGACATGGATCGAAGTGGAGGGCCACCATTACGACCCACCGGCGGTGGCTTTATCATTCGAGCATCTAGTCAAGCCCCAATTTGGGCTTGGTGAAACCGATCCTGCAGCAGTGGCGGAGAACGAAACCAAGCTGGGGAAAGTTCTTGACATCTACGAGAATCAGCTTAGCAAAAACAAGTTCTTGGCTGGGGATGAGTTCAGTTTGGCGGATTTGCACCATATCCCCAATGTGAGCTATTTGTCCATGACGCCATCGAAGAAGCTGTTCGAGTCACGGCCACGTGTCAATGCTTGGGTGGCCGAGATCACTGCAAGGCCTTCGTGGGCTAAGGTTGTTGCCTTGCGCAATCAGAGTTAAGAGCTTTGGTACTATACCAACATGAACAGGTAGTTTTTGTTTTGTTGTTGGCTTGTAAATGGTGCTTAGGGTAAAAAAAAATAAATGTTTTGGTCACTTTTACCCTAAGTACTTTTGCTTCATGGGTGTAGTTTGTTTTTGTTTCTCATCATGGATGTATGAAAATGGAAATAATTGCCTTGGCTCACAAAGTTCCCCTTTGGGTAATGTTGATAATTTGTTTGTTTCTGACTGTCAAACAAGTTTAAATTTATTGAACTTATGACCAACATTAAAATATGATTAAAAATTACTTTGATCCTTAAACGATTCGGACTCTGAATCTTGAAGTATAAAAATTTAGTTTTTGTGCTTTTAATTTTGTTCTAATTTTGTCCATGTAACAATTTATTCACTATATTCTTAAATTTGTAACAATTTAGCTCTTATCATGAAAAAGTCAAACTTAATTATCAATTTTGATTAGGTAACCAACTTAGTCTTTATAATTTATAAAGATATTTAATTCTTAACCAGTTTAAC

mRNA sequence

GATTTTGATCCATATACTAATTATGGGTTTCTTGAGAAAAGAAAGAAAAAGAACTTTATGAAACAGTTGATCAGCCGCGAATCTGAAATCGACCGCCATTAACGATCCGCGTTCTACGACCCCATCGATTTCCCATTTCGCTAAGAGATTCAGCAATGTCAAATTAATCCTTCCCTCATTCAATTCTCTATAAGAGAACCTTCAACAAATTCTTCTTCCCCACTCAAACCCATCTCAATTTCTTCGAATAATGGCGAGCATCAGAGTCCATGGAAGCCCCATTTCCACTGCCACAGCTAGAATTCTCGCTTGTCTTTACGAGAAAGATCTCGATTACGAGTTCGTTCTTCTGGACATGAAAAACAACGAGCACAAGAAACATCCCTTTCTCTCCATTAACCCGTTTGGTCAAGTTCCTGGGTTTCAAGATGGGGATCTCACCATTTTCGAATCAAGGGCCATTACTCAATACTTGGCCAAGGCCTACGCCAGCAAGGGAACCCAATTGATCTCCGACGACCCAAAGAAAGCGGCGATCATATTGACATGGATCGAAGTGGAGGGCCACCATTACGACCCACCGGCGGTGGCTTTATCATTCGAGCATCTAGTCAAGCCCCAATTTGGGCTTGGTGAAACCGATCCTGCAGCAGTGGCGGAGAACGAAACCAAGCTGGGGAAAGTTCTTGACATCTACGAGAATCAGCTTAGCAAAAACAAGTTCTTGGCTGGGGATGAGTTCAGTTTGGCGGATTTGCACCATATCCCCAATGTGAGCTATTTGTCCATGACGCCATCGAAGAAGCTGTTCGAGTCACGGCCACGTGTCAATGCTTGGGTGGCCGAGATCACTGCAAGGCCTTCGTGGGCTAAGGTTGTTGCCTTGCGCAATCAGAGTTAAGAGCTTTGGTACTATACCAACATGAACAGGTAGTTTTTGTTTTGTTGTTGGCTTGTAAATGGTGCTTAGGGTAAAAAAAAATAAATGTTTTGGTCACTTTTACCCTAAGTACTTTTGCTTCATGGGTGTAGTTTGTTTTTGTTTCTCATCATGGATGTATGAAAATGGAAATAATTGCCTTGGCTCACAAAGTTCCCCTTTGGGTAATGTTGATAATTTGTTTGTTTCTGACTGTCAAACAAGTTTAAATTTATTGAACTTATGACCAACATTAAAATATGATTAAAAATTACTTTGATCCTTAAACGATTCGGACTCTGAATCTTGAAGTATAAAAATTTAGTTTTTGTGCTTTTAATTTTGTTCTAATTTTGTCCATGTAACAATTTATTCACTATATTCTTAAATTTGTAACAATTTAGCTCTTATCATGAAAAAGTCAAACTTAATTATCAATTTTGATTAGGTAACCAACTTAGTCTTTATAATTTATAAAGATATTTAATTCTTAACCAGTTTAAC

Coding sequence (CDS)

ATGGCGAGCATCAGAGTCCATGGAAGCCCCATTTCCACTGCCACAGCTAGAATTCTCGCTTGTCTTTACGAGAAAGATCTCGATTACGAGTTCGTTCTTCTGGACATGAAAAACAACGAGCACAAGAAACATCCCTTTCTCTCCATTAACCCGTTTGGTCAAGTTCCTGGGTTTCAAGATGGGGATCTCACCATTTTCGAATCAAGGGCCATTACTCAATACTTGGCCAAGGCCTACGCCAGCAAGGGAACCCAATTGATCTCCGACGACCCAAAGAAAGCGGCGATCATATTGACATGGATCGAAGTGGAGGGCCACCATTACGACCCACCGGCGGTGGCTTTATCATTCGAGCATCTAGTCAAGCCCCAATTTGGGCTTGGTGAAACCGATCCTGCAGCAGTGGCGGAGAACGAAACCAAGCTGGGGAAAGTTCTTGACATCTACGAGAATCAGCTTAGCAAAAACAAGTTCTTGGCTGGGGATGAGTTCAGTTTGGCGGATTTGCACCATATCCCCAATGTGAGCTATTTGTCCATGACGCCATCGAAGAAGCTGTTCGAGTCACGGCCACGTGTCAATGCTTGGGTGGCCGAGATCACTGCAAGGCCTTCGTGGGCTAAGGTTGTTGCCTTGCGCAATCAGAGTTAA

Protein sequence

MASIRVHGSPISTATARILACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQDGDLTIFESRAITQYLAKAYASKGTQLISDDPKKAAIILTWIEVEGHHYDPPAVALSFEHLVKPQFGLGETDPAAVAENETKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNVSYLSMTPSKKLFESRPRVNAWVAEITARPSWAKVVALRNQS

Homology

[Close]

BLAST of Bhi09M000691 vs. TAIR 10
Match: AT2G47730.1 (glutathione S-transferase phi 8 )

HSP 1 Score: 258.5 bits (659), Expect = 4.9e-69
Identity = 125/216 (57.87%), Postives = 164/216 (75.93%), Query Frame = 0


Query: 1   MASIRVHGSPISTATARILACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQD 60
           MASI+VHG P+STAT R+LA LYEKDL +E + +DM+   HK+   L++NPFGQ+P  +D
Sbjct: 49  MASIKVHGVPMSTATMRVLATLYEKDLQFELIPVDMRAGAHKQEAHLALNPFGQIPALED 108

Query: 61  GDLTIFESRAITQYLAKAYASKGTQLISDDPKKA-AIILTWIEVEGHHYDPPAVALSFEH 120
           GDLT+FESRAITQYLA+ Y+ KG +LIS D KK  A    W++VEG  +DP A  L+FE 
Sbjct: 109 GDLTLFESRAITQYLAEEYSEKGEKLISQDCKKVKATTNVWLQVEGQQFDPNASKLAFER 168

Query: 121 LVKPQFGLGETDPAAVAENETKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNVSYLS 180
           + K  FG+  TDPAAV E E KL KVLD+YE +L+K++FLAGD F+LADLHH+P + YL 
Sbjct: 169 VFKGMFGM-TTDPAAVQELEGKLQKVLDVYEARLAKSEFLAGDSFTLADLHHLPAIHYLL 228

Query: 181 MTPSKKLFESRPRVNAWVAEITARPSWAKVVALRNQ 216
            T SK LF+SRP+V+ W+ +I+ARP+WAKV+ L+ Q
Sbjct: 229 GTDSKVLFDSRPKVSEWIKKISARPAWAKVIDLQKQ 263

[Close]

BLAST of Bhi09M000691 vs. TAIR 10
Match: AT4G02520.1 (glutathione S-transferase PHI 2 )

HSP 1 Score: 239.2 bits (609), Expect = 3.1e-63
Identity = 120/212 (56.60%), Postives = 152/212 (71.70%), Query Frame = 0


Query: 1   MASIRVHGSPISTATARILACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQD 60
           MA I+V G P S AT R+L  L+EK+LD+E V +++K+ EHKK PFLS NPFGQVP F+D
Sbjct: 1   MAGIKVFGHPASIATRRVLIALHEKNLDFELVHVELKDGEHKKEPFLSRNPFGQVPAFED 60

Query: 61  GDLTIFESRAITQYLAKAYASKGTQLISDDPK---KAAIILTWIEVEGHHYDPPAVALSF 120
           GDL +FESRAITQY+A  Y ++GT L+  D K   + AI+   ++VE H +DP A  L+F
Sbjct: 61  GDLKLFESRAITQYIAHRYENQGTNLLQTDSKNISQYAIMAIGMQVEDHQFDPVASKLAF 120

Query: 121 EHLVKPQFGLGETDPAAVAENETKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNVSY 180
           E + K  +GL  TD A VAE E KL KVLD+YE +L + K+LAG+ F+L DLHHIP + Y
Sbjct: 121 EQIFKSIYGL-TTDEAVVAEEEAKLAKVLDVYEARLKEFKYLAGETFTLTDLHHIPAIQY 180

Query: 181 LSMTPSKKLFESRPRVNAWVAEITARPSWAKV 210
           L  TP+KKLF  RPRVN WVAEIT RP+  KV
Sbjct: 181 LLGTPTKKLFTERPRVNEWVAEITKRPASEKV 211

[Close]

BLAST of Bhi09M000691 vs. TAIR 10
Match: AT2G02930.1 (glutathione S-transferase F3 )

HSP 1 Score: 238.8 bits (608), Expect = 4.0e-63
Identity = 120/213 (56.34%), Postives = 154/213 (72.30%), Query Frame = 0


Query: 1   MASIRVHGSPISTATARILACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQD 60
           MA I+V G P ST+T R+L  L+EK+LD+E V +++K+ EHKK PFLS NPFGQVP F+D
Sbjct: 1   MAGIKVFGHPASTSTRRVLIALHEKNLDFELVHVELKDGEHKKEPFLSRNPFGQVPAFED 60

Query: 61  GDLTIFESRAITQYLAKAYASKGTQLISDDPK---KAAIILTWIEVEGHHYDPPAVALSF 120
           GDL +FESRAITQY+A  Y ++GT L+  D K   + AI+   I+VE H +DP A  L++
Sbjct: 61  GDLKLFESRAITQYIAHRYENQGTNLLPADSKNIAQYAIMSIGIQVEAHQFDPVASKLAW 120

Query: 121 EHLVKPQFGLGETDPAAVAENETKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNVSY 180
           E + K  +GL  TD A VAE E KL KVLD+YE +L + K+LAG+ F+L DLHHIP + Y
Sbjct: 121 EQVFKFNYGL-NTDQAVVAEEEAKLAKVLDVYEARLKEFKYLAGETFTLTDLHHIPVIQY 180

Query: 181 LSMTPSKKLFESRPRVNAWVAEITARPSWAKVV 211
           L  TP+KKLF  RPRVN WVAEIT RP+  KV+
Sbjct: 181 LLGTPTKKLFTERPRVNEWVAEITKRPASEKVL 212

[Close]

BLAST of Bhi09M000691 vs. TAIR 10
Match: AT1G02920.1 (glutathione S-transferase 7 )

HSP 1 Score: 236.9 bits (603), Expect = 1.5e-62
Identity = 114/210 (54.29%), Postives = 152/210 (72.38%), Query Frame = 0


Query: 1   MASIRVHGSPISTATARILACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQD 60
           MA I+V G P STAT R+L  L+EK+LD+EFV +++K+ EHKK PF+  NPFG+VP F+D
Sbjct: 1   MAGIKVFGHPASTATRRVLIALHEKNLDFEFVHIELKDGEHKKEPFIFRNPFGKVPAFED 60

Query: 61  GDLTIFESRAITQYLAKAYASKGTQLISDDPKKAAIILTWIEVEGHHYDPPAVALSFEHL 120
           GD  +FESRAITQY+A  Y+ KG QL+S   K  A I   IE+E H +DP    L +E +
Sbjct: 61  GDFKLFESRAITQYIAHFYSDKGNQLVSLGSKDIAGIAMGIEIESHEFDPVGSKLVWEQV 120

Query: 121 VKPQFGLGETDPAAVAENETKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNVSYLSM 180
           +KP +G+  TD   V E E KL KVLD+YE++L ++K+LA D+F+L DLH IP + YL  
Sbjct: 121 LKPLYGM-TTDKTVVEEEEAKLAKVLDVYEHRLGESKYLASDKFTLVDLHTIPVIQYLLG 180

Query: 181 TPSKKLFESRPRVNAWVAEITARPSWAKVV 211
           TP+KKLF+ RP V+AWVA+IT+RPS  KV+
Sbjct: 181 TPTKKLFDERPHVSAWVADITSRPSAKKVL 209

[Close]

BLAST of Bhi09M000691 vs. TAIR 10
Match: AT1G02930.1 (glutathione S-transferase 6 )

HSP 1 Score: 232.3 bits (591), Expect = 3.8e-61
Identity = 113/210 (53.81%), Postives = 151/210 (71.90%), Query Frame = 0


Query: 1   MASIRVHGSPISTATARILACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQD 60
           MA I+V G P STAT R+L  L+EK++D+EFV +++K+ EHKK PF+  NPFG+VP F+D
Sbjct: 1   MAGIKVFGHPASTATRRVLIALHEKNVDFEFVHVELKDGEHKKEPFILRNPFGKVPAFED 60

Query: 61  GDLTIFESRAITQYLAKAYASKGTQLISDDPKKAAIILTWIEVEGHHYDPPAVALSFEHL 120
           GD  IFESRAITQY+A  ++ KG  L+S   K  AII   IE+E H +DP    L +E +
Sbjct: 61  GDFKIFESRAITQYIAHEFSDKGNNLLSTG-KDMAIIAMGIEIESHEFDPVGSKLVWEQV 120

Query: 121 VKPQFGLGETDPAAVAENETKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNVSYLSM 180
           +KP +G+  TD   V E E KL KVLD+YE++L ++K+LA D F+L DLH IP + YL  
Sbjct: 121 LKPLYGM-TTDKTVVEEEEAKLAKVLDVYEHRLGESKYLASDHFTLVDLHTIPVIQYLLG 180

Query: 181 TPSKKLFESRPRVNAWVAEITARPSWAKVV 211
           TP+KKLF+ RP V+AWVA+IT+RPS  KV+
Sbjct: 181 TPTKKLFDERPHVSAWVADITSRPSAQKVL 208

[Close]

BLAST of Bhi09M000691 vs. ExPASy Swiss-Prot
Match: P30109 (Glutathione S-transferase PARB OS=Nicotiana tabacum OX=4097 GN=PARB PE=2 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 6.7e-71
Identity = 124/206 (60.19%), Postives = 160/206 (77.67%), Query Frame = 0


Query: 3   SIRVHGSPISTATARILACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQDGD 62
           +I+VHGSP+STAT R+ ACL EK+LD+EFV +DM + EHKKHP+LS+NPFGQVP F+DGD
Sbjct: 2   AIKVHGSPMSTATMRVAACLIEKELDFEFVPVDMASGEHKKHPYLSLNPFGQVPAFEDGD 61

Query: 63  LTIFESRAITQYLAKAYASKGTQLISDDPKKAAIILTWIEVEGHHYDPPAVALSFEHLVK 122
           L +FESRAITQY+A  YA  G QLI  DPKK   +  W+EVEG  ++PPA  L++E  +K
Sbjct: 62  LKLFESRAITQYIAHVYADNGYQLILQDPKKMPSMSVWMEVEGQKFEPPATKLTWELGIK 121

Query: 123 PQFGLGETDPAAVAENETKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNVSYLSMTP 182
           P  G+  TD AAV E+E +L KVLDIYE QL+++K+L GD F+L DLHHIPN+ YL  + 
Sbjct: 122 PIIGM-TTDDAAVKESEAQLSKVLDIYETQLAESKYLGGDSFTLVDLHHIPNIYYLMSSK 181

Query: 183 SKKLFESRPRVNAWVAEITARPSWAK 209
            K++F+SRPRV+AW A+I ARP+W K
Sbjct: 182 VKEVFDSRPRVSAWCADILARPAWVK 206

[Close]

BLAST of Bhi09M000691 vs. ExPASy Swiss-Prot
Match: P46440 (Glutathione S-transferase APIC OS=Nicotiana tabacum OX=4097 GN=APIC PE=2 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 2.8e-69
Identity = 123/206 (59.71%), Postives = 158/206 (76.70%), Query Frame = 0


Query: 3   SIRVHGSPISTATARILACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQDGD 62
           +I+VHGSP+STAT R+ ACL EKDLD+E V +DM + EHKKHP+LS+NPFGQVP F+DGD
Sbjct: 2   AIKVHGSPMSTATMRVAACLIEKDLDFELVPVDMVSGEHKKHPYLSLNPFGQVPAFEDGD 61

Query: 63  LTIFESRAITQYLAKAYASKGTQLISDDPKKAAIILTWIEVEGHHYDPPAVALSFEHLVK 122
           L +FESRAITQY+A  YA  G QLI  DPKK  I+  W+EVEG  ++P A  L++E  +K
Sbjct: 62  LKLFESRAITQYIAHVYADNGYQLILQDPKKMPIMSVWMEVEGQKFEPHASKLTWELGIK 121

Query: 123 PQFGLGETDPAAVAENETKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNVSYLSMTP 182
           P  G+  TD  AV E+E +L KVLDIYE +L+++K+L GD F+L DLHHIPN+ YL  T 
Sbjct: 122 PIIGM-TTDDDAVKESEVQLSKVLDIYETRLAESKYLGGDSFTLVDLHHIPNIYYLMSTK 181

Query: 183 SKKLFESRPRVNAWVAEITARPSWAK 209
            K++F+SRPRV+AW A+I ARP+W K
Sbjct: 182 VKEVFDSRPRVSAWCADILARPAWVK 206

[Close]

BLAST of Bhi09M000691 vs. ExPASy Swiss-Prot
Match: Q96266 (Glutathione S-transferase F8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=GSTF8 PE=1 SV=3)

HSP 1 Score: 258.5 bits (659), Expect = 6.9e-68
Identity = 125/216 (57.87%), Postives = 164/216 (75.93%), Query Frame = 0


Query: 1   MASIRVHGSPISTATARILACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQD 60
           MASI+VHG P+STAT R+LA LYEKDL +E + +DM+   HK+   L++NPFGQ+P  +D
Sbjct: 49  MASIKVHGVPMSTATMRVLATLYEKDLQFELIPVDMRAGAHKQEAHLALNPFGQIPALED 108

Query: 61  GDLTIFESRAITQYLAKAYASKGTQLISDDPKKA-AIILTWIEVEGHHYDPPAVALSFEH 120
           GDLT+FESRAITQYLA+ Y+ KG +LIS D KK  A    W++VEG  +DP A  L+FE 
Sbjct: 109 GDLTLFESRAITQYLAEEYSEKGEKLISQDCKKVKATTNVWLQVEGQQFDPNASKLAFER 168

Query: 121 LVKPQFGLGETDPAAVAENETKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNVSYLS 180
           + K  FG+  TDPAAV E E KL KVLD+YE +L+K++FLAGD F+LADLHH+P + YL 
Sbjct: 169 VFKGMFGM-TTDPAAVQELEGKLQKVLDVYEARLAKSEFLAGDSFTLADLHHLPAIHYLL 228

Query: 181 MTPSKKLFESRPRVNAWVAEITARPSWAKVVALRNQ 216
            T SK LF+SRP+V+ W+ +I+ARP+WAKV+ L+ Q
Sbjct: 229 GTDSKVLFDSRPKVSEWIKKISARPAWAKVIDLQKQ 263

[Close]

BLAST of Bhi09M000691 vs. ExPASy Swiss-Prot
Match: P46423 (Glutathione S-transferase OS=Hyoscyamus muticus OX=35626 PE=1 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 5.9e-67
Identity = 117/207 (56.52%), Postives = 158/207 (76.33%), Query Frame = 0


Query: 4   IRVHGSPISTATARILACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQDGDL 63
           +++HG  +S A  R++A L EKDLD+E V ++M+  +HKK PF+++NPFGQVP F+DGDL
Sbjct: 3   MKLHGPAMSPAVMRVIATLKEKDLDFELVPVNMQAGDHKKEPFITLNPFGQVPAFEDGDL 62

Query: 64  TIFESRAITQYLAKAYASKGTQLISDDPKKAAIILTWIEVEGHHYDPPAVALSFEHLVKP 123
            +FESRAITQY+A  YA KG QL+++DPKK AI+  W+EVE   +DP A  L+FE ++KP
Sbjct: 63  KLFESRAITQYIAHTYADKGNQLLANDPKKMAIMSVWMEVESQKFDPVASKLTFEIVIKP 122

Query: 124 QFGLGETDPAAVAENETKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNVSYLSMTPS 183
             G+  TD AAVAENE KLGKVLD+YE++L  +K+L GD F+LADLHH P ++YL  T  
Sbjct: 123 MLGM-VTDDAAVAENEEKLGKVLDVYESRLKDSKYLGGDSFTLADLHHAPAMNYLMGTKV 182

Query: 184 KKLFESRPRVNAWVAEITARPSWAKVV 211
           K LF+SRP V+AW A+I ARP+W+K +
Sbjct: 183 KSLFDSRPHVSAWCADILARPAWSKAI 208

[Close]

BLAST of Bhi09M000691 vs. ExPASy Swiss-Prot
Match: P46422 (Glutathione S-transferase F2 OS=Arabidopsis thaliana OX=3702 GN=GSTF2 PE=1 SV=3)

HSP 1 Score: 239.2 bits (609), Expect = 4.4e-62
Identity = 120/212 (56.60%), Postives = 152/212 (71.70%), Query Frame = 0


Query: 1   MASIRVHGSPISTATARILACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQD 60
           MA I+V G P S AT R+L  L+EK+LD+E V +++K+ EHKK PFLS NPFGQVP F+D
Sbjct: 1   MAGIKVFGHPASIATRRVLIALHEKNLDFELVHVELKDGEHKKEPFLSRNPFGQVPAFED 60

Query: 61  GDLTIFESRAITQYLAKAYASKGTQLISDDPK---KAAIILTWIEVEGHHYDPPAVALSF 120
           GDL +FESRAITQY+A  Y ++GT L+  D K   + AI+   ++VE H +DP A  L+F
Sbjct: 61  GDLKLFESRAITQYIAHRYENQGTNLLQTDSKNISQYAIMAIGMQVEDHQFDPVASKLAF 120

Query: 121 EHLVKPQFGLGETDPAAVAENETKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNVSY 180
           E + K  +GL  TD A VAE E KL KVLD+YE +L + K+LAG+ F+L DLHHIP + Y
Sbjct: 121 EQIFKSIYGL-TTDEAVVAEEEAKLAKVLDVYEARLKEFKYLAGETFTLTDLHHIPAIQY 180

Query: 181 LSMTPSKKLFESRPRVNAWVAEITARPSWAKV 210
           L  TP+KKLF  RPRVN WVAEIT RP+  KV
Sbjct: 181 LLGTPTKKLFTERPRVNEWVAEITKRPASEKV 211

[Close]

BLAST of Bhi09M000691 vs. ExPASy TrEMBL
Match: A0A5A7VLC1 (Glutathione transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G00310 PE=3 SV=1)

HSP 1 Score: 411.8 bits (1057), Expect = 1.8e-111
Identity = 198/216 (91.67%), Postives = 205/216 (94.91%), Query Frame = 0


Query: 1   MASIRVHGSPISTATARILACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQD 60
           MASI+VHGSPISTAT+R+LACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQD
Sbjct: 1   MASIKVHGSPISTATSRVLACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQD 60

Query: 61  GDLTIFESRAITQYLAKAYASKGTQLISDDPKKAAIILTWIEVEGHHYDPPAVALSFEHL 120
           GDLTIFESRAITQYLA AYA+KGTQLI +DPKKAAI+ TWIEVEGHHYDPP VALSFEHL
Sbjct: 61  GDLTIFESRAITQYLATAYANKGTQLIPNDPKKAAIVSTWIEVEGHHYDPPGVALSFEHL 120

Query: 121 VKPQFGLGETDPAAVAENETKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNVSYLSM 180
           VKP FGLGETDPAAVAENE KLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPN+SYL  
Sbjct: 121 VKPLFGLGETDPAAVAENEAKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNISYLLA 180

Query: 181 TPSKKLFESRPRVNAWVAEITARPSWAKVVALRNQS 217
           TPSKKLFESRPRVNAWV EI ARPSWAKV ALRNQS
Sbjct: 181 TPSKKLFESRPRVNAWVTEILARPSWAKVTALRNQS 216

[Close]

BLAST of Bhi09M000691 vs. ExPASy TrEMBL
Match: A0A1S3BT14 (Glutathione transferase OS=Cucumis melo OX=3656 GN=LOC103492841 PE=3 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 4.0e-111
Identity = 197/216 (91.20%), Postives = 205/216 (94.91%), Query Frame = 0


Query: 1   MASIRVHGSPISTATARILACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQD 60
           MASI+VHGSPISTAT+R+LACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQD
Sbjct: 1   MASIKVHGSPISTATSRVLACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQD 60

Query: 61  GDLTIFESRAITQYLAKAYASKGTQLISDDPKKAAIILTWIEVEGHHYDPPAVALSFEHL 120
           GDLTIFESRAITQYLA AYA+KGTQLI +DPKKAAI+ TWIEVEGHHYDPP VALSFEHL
Sbjct: 61  GDLTIFESRAITQYLATAYANKGTQLIPNDPKKAAIVSTWIEVEGHHYDPPGVALSFEHL 120

Query: 121 VKPQFGLGETDPAAVAENETKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNVSYLSM 180
           VKP FGLGETDPAAVAENE KLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPN+SYL  
Sbjct: 121 VKPLFGLGETDPAAVAENEAKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNISYLLA 180

Query: 181 TPSKKLFESRPRVNAWVAEITARPSWAKVVALRNQS 217
           TPSKKLFESRPRVNAWV +I ARPSWAKV ALRNQS
Sbjct: 181 TPSKKLFESRPRVNAWVTDILARPSWAKVTALRNQS 216

[Close]

BLAST of Bhi09M000691 vs. ExPASy TrEMBL
Match: A0A0A0K601 (Glutathione transferase OS=Cucumis sativus OX=3659 GN=Csa_7G395800 PE=3 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 5.3e-111
Identity = 197/216 (91.20%), Postives = 204/216 (94.44%), Query Frame = 0


Query: 1   MASIRVHGSPISTATARILACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQD 60
           MASI+VHGSPISTATAR+L CLYEKDLDYEFVL+DMKNNEHKKHPFLSINPFGQVPGFQD
Sbjct: 1   MASIKVHGSPISTATARVLVCLYEKDLDYEFVLVDMKNNEHKKHPFLSINPFGQVPGFQD 60

Query: 61  GDLTIFESRAITQYLAKAYASKGTQLISDDPKKAAIILTWIEVEGHHYDPPAVALSFEHL 120
           GDLTIFESRAITQYLAK YA+KG QLI +DPKKAAI+ TWIEVEGHHYDPP VALSFEHL
Sbjct: 61  GDLTIFESRAITQYLAKTYANKGNQLIPNDPKKAAIVSTWIEVEGHHYDPPGVALSFEHL 120

Query: 121 VKPQFGLGETDPAAVAENETKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNVSYLSM 180
           VKP FGLGETDPAAVAENE KLGKVLDIYENQLSKNKFLAGDEFSLADLHH+PNVSYL  
Sbjct: 121 VKPLFGLGETDPAAVAENEAKLGKVLDIYENQLSKNKFLAGDEFSLADLHHLPNVSYLLA 180

Query: 181 TPSKKLFESRPRVNAWVAEITARPSWAKVVALRNQS 217
           TPSKKLFESRPRVNAWV EI ARPSWAKVVALRNQS
Sbjct: 181 TPSKKLFESRPRVNAWVTEIIARPSWAKVVALRNQS 216

[Close]

BLAST of Bhi09M000691 vs. ExPASy TrEMBL
Match: A0A6J1KSP9 (Glutathione transferase OS=Cucurbita maxima OX=3661 GN=LOC111497881 PE=3 SV=1)

HSP 1 Score: 401.4 bits (1030), Expect = 2.4e-108
Identity = 193/215 (89.77%), Postives = 204/215 (94.88%), Query Frame = 0


Query: 1   MASIRVHGSPISTATARILACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQD 60
           MASI+VHGSPISTATAR+LACLYEKDL+Y+FVLLDMKNNEHKK PFLSINPFGQVPGFQD
Sbjct: 1   MASIKVHGSPISTATARVLACLYEKDLEYQFVLLDMKNNEHKKEPFLSINPFGQVPGFQD 60

Query: 61  GDLTIFESRAITQYLAKAYASKGTQLISDDPKKAAIILTWIEVEGHHYDPPAVALSFEHL 120
           GDLTIFESRAITQYLA AYASKG QL+SDDPKKAAII TWIEVEGHHYDPP VALS+E+L
Sbjct: 61  GDLTIFESRAITQYLASAYASKGNQLLSDDPKKAAIISTWIEVEGHHYDPPGVALSYEYL 120

Query: 121 VKPQFGLGETDPAAVAENETKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNVSYLSM 180
           VKP FGLGETDPAAVAENE KLGKVLDIYENQLSK+KFLAG+EFSLADLHHIPNV YL  
Sbjct: 121 VKPLFGLGETDPAAVAENEAKLGKVLDIYENQLSKSKFLAGNEFSLADLHHIPNVHYLLA 180

Query: 181 TPSKKLFESRPRVNAWVAEITARPSWAKVVALRNQ 216
           TPSKKLFESRPRVNAWVAEITARPSW KV+A+RNQ
Sbjct: 181 TPSKKLFESRPRVNAWVAEITARPSWGKVLAMRNQ 215

[Close]

BLAST of Bhi09M000691 vs. ExPASy TrEMBL
Match: A0A6J1H6I2 (Glutathione transferase OS=Cucurbita moschata OX=3662 GN=LOC111460651 PE=3 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 5.5e-108
Identity = 193/215 (89.77%), Postives = 202/215 (93.95%), Query Frame = 0


Query: 1   MASIRVHGSPISTATARILACLYEKDLDYEFVLLDMKNNEHKKHPFLSINPFGQVPGFQD 60
           MASI+VHGSPISTATAR+LACLYEKDL+Y+FVLLDMKNNEHKK PFLSINPFGQVPGFQD
Sbjct: 1   MASIKVHGSPISTATARVLACLYEKDLEYQFVLLDMKNNEHKKEPFLSINPFGQVPGFQD 60

Query: 61  GDLTIFESRAITQYLAKAYASKGTQLISDDPKKAAIILTWIEVEGHHYDPPAVALSFEHL 120
           GDLTIFESRAITQYLA AYASKG QL+SDDPKKAAII TWIEVEGHHYDPP VALS+E+L
Sbjct: 61  GDLTIFESRAITQYLASAYASKGNQLLSDDPKKAAIISTWIEVEGHHYDPPGVALSYEYL 120

Query: 121 VKPQFGLGETDPAAVAENETKLGKVLDIYENQLSKNKFLAGDEFSLADLHHIPNVSYLSM 180
           VKP FGLGETDPAAVAENE KLGKVLDIYENQL K+KFLAGD FSLADLHHIPNV YL  
Sbjct: 121 VKPLFGLGETDPAAVAENEAKLGKVLDIYENQLGKSKFLAGDTFSLADLHHIPNVHYLLA 180

Query: 181 TPSKKLFESRPRVNAWVAEITARPSWAKVVALRNQ 216
           TPSKKLFESRPRVNAWVAEITARPSW KV+ALRNQ
Sbjct: 181 TPSKKLFESRPRVNAWVAEITARPSWGKVLALRNQ 215

The following BLAST results are available for this feature:

BLAST of Bhi09M000691 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G47730.1	4.9e-69	57.87	glutathione S-transferase phi 8	[more]
AT4G02520.1	3.1e-63	56.60	glutathione S-transferase PHI 2	[more]
AT2G02930.1	4.0e-63	56.34	glutathione S-transferase F3	[more]
AT1G02920.1	1.5e-62	54.29	glutathione S-transferase 7	[more]
AT1G02930.1	3.8e-61	53.81	glutathione S-transferase 6	[more]

BLAST of Bhi09M000691 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P30109	6.7e-71	60.19	Glutathione S-transferase PARB OS=Nicotiana tabacum OX=4097 GN=PARB PE=2 SV=1	[more]
P46440	2.8e-69	59.71	Glutathione S-transferase APIC OS=Nicotiana tabacum OX=4097 GN=APIC PE=2 SV=1	[more]
Q96266	6.9e-68	57.87	Glutathione S-transferase F8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
P46423	5.9e-67	56.52	Glutathione S-transferase OS=Hyoscyamus muticus OX=35626 PE=1 SV=1	[more]
P46422	4.4e-62	56.60	Glutathione S-transferase F2 OS=Arabidopsis thaliana OX=3702 GN=GSTF2 PE=1 SV=3	[more]

BLAST of Bhi09M000691 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VLC1	1.8e-111	91.67	Glutathione transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3BT14	4.0e-111	91.20	Glutathione transferase OS=Cucumis melo OX=3656 GN=LOC103492841 PE=3 SV=1	[more]
A0A0A0K601	5.3e-111	91.20	Glutathione transferase OS=Cucumis sativus OX=3659 GN=Csa_7G395800 PE=3 SV=1	[more]
A0A6J1KSP9	2.4e-108	89.77	Glutathione transferase OS=Cucurbita maxima OX=3661 GN=LOC111497881 PE=3 SV=1	[more]
A0A6J1H6I2	5.5e-108	89.77	Glutathione transferase OS=Cucurbita moschata OX=3662 GN=LOC111460651 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004045	Glutathione S-transferase, N-terminal	PFAM	PF02798	GST_N	coord: 12..76 e-value: 4.3E-18 score: 65.4
IPR004045	Glutathione S-transferase, N-terminal	PROSITE	PS50404	GST_NTER	coord: 2..83 score: 23.015085
IPR004046	Glutathione S-transferase, C-terminal	PFAM	PF00043	GST_C	coord: 112..204 e-value: 1.3E-17 score: 63.7
None	No IPR available	GENE3D	1.20.1050.10		coord: 90..214 e-value: 8.5E-48 score: 163.0
None	No IPR available	GENE3D	3.40.30.10	Glutaredoxin	coord: 1..87 e-value: 1.2E-27 score: 97.9
None	No IPR available	SFLD	SFLDG00358	Main_(cytGST)	coord: 1..212 e-value: 0.0 score: 295.1
None	No IPR available	SFLD	SFLDG01154	Main.5:_Phi-like	coord: 1..212 e-value: 0.0 score: 295.1
None	No IPR available	PANTHER	PTHR43900:SF47	GLUTATHIONE S-TRANSFERASE F6-RELATED	coord: 4..215
None	No IPR available	PANTHER	PTHR43900	GLUTATHIONE S-TRANSFERASE RHO	coord: 4..215
None	No IPR available	CDD	cd03053	GST_N_Phi	coord: 4..77 e-value: 1.38511E-33 score: 113.514
IPR010987	Glutathione S-transferase, C-terminal-like	PROSITE	PS50405	GST_CTER	coord: 90..216 score: 22.489614
IPR034347	Glutathione S-transferases Phi, C-terminal	CDD	cd03187	GST_C_Phi	coord: 92..210 e-value: 2.30358E-63 score: 190.517
IPR036282	Glutathione S-transferase, C-terminal domain superfamily	SUPERFAMILY	47616	GST C-terminal domain-like	coord: 74..212
IPR036249	Thioredoxin-like superfamily	SUPERFAMILY	52833	Thioredoxin-like	coord: 2..82

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Bhi09G000691	Bhi09G000691	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi09M000691.utr5p1	Bhi09M000691.utr5p1	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi09M000691.exon1	Bhi09M000691.exon1	exon
Bhi09M000691.exon2	Bhi09M000691.exon2	exon
Bhi09M000691.exon3	Bhi09M000691.exon3	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Bhi09M000691	cds.Bhi09M000691	CDS
cds.Bhi09M000691	cds.Bhi09M000691_2	CDS
cds.Bhi09M000691	cds.Bhi09M000691_3	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi09M000691.utr3p1	Bhi09M000691.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Bhi09M000691	Bhi09M000691-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006749	glutathione metabolic process
biological_process	GO:0009407	toxin catabolic process
cellular_component	GO:0005829	cytosol
molecular_function	GO:0005515	protein binding
molecular_function	GO:0043295	glutathione binding
molecular_function	GO:0004364	glutathione transferase activity