Bhi08M001165 (mRNA) Wax gourd (B227) v1

Overview
NameBhi08M001165
TypemRNA
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionPectate lyase
Locationchr8: 41879522 .. 41884725 (-)
Sequence length1882
RNA-Seq ExpressionBhi08M001165
SyntenyBhi08M001165
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AACTCTCATTTAAATGATCTTTTGAGTAAAACCATTTAGTTTTTTTGCTCTCATACATTCAACTGTTTAAAACTACATATCAATATATCATAGCACAACACAATTTCGAACAATTTATGCTTGTAATGCACCTACTAGATGCTCCTTCACGATGGCACGTGAGATTCCCCCTCCCCCTTTCCCCAAAAAAAGCAACCCACAAAAGTTCATTCCAAACGAAAACAACAAGCTCTCCCCTTTCCCCCAAAATCACTTCCATTACTCTACTCATCACTGACTCATCATTTCTTTCTTTCTCCTCCTCTACCTACTTCTTCACAGATCTTCAATGGAGATCCCAATATTCAGCAGGAGCTTTACAATGGATGAAAAAGAGGGTCTTCACGGTACCAATTTCTCGCTGCAATCGCTTAGTTACTGTGAACTATGGCCAAGGGCCTGTTTTTGCCCTTGTGGAAGAAGCTTCTCAATCCCAATGTCTTATTCTGCACTTTCTGAATGGGTGGACTTTGTTGCTAGAGAATCTGATCCCCTCATCTCATAAAAAGTAATTTTCTTTTCTCACCGATGCTTCTTCTTCTGTCCTTTGGCATATTTTTTTTTTCCCTCTCCGTGACATCATTTCAAATTTTCAATGCTTAAATATATGATAATGGGTGTGAAAAAGGATGTGTTTCTCTTCAACTTTCTCCATTCTCTGACTAATATCACCCCTCCAGACAGTTGCTTACCTTTGGGGCTCTAATCCATATCTATAGTGTTTATGTCTATCCCACTGAAAAAGAAACTTGGATTCCATATAGTTTGGACAAACTCTAAAATGGGTTGTGATCAAGTACATCAAACCGTGTGGTTAAAGTGTTGATATAATGAAATGAGGTTTTTGTATGGATGATCCATTTCTAGAGGCATTATTGTAGAATAATCCAAATCCTAAAAAACGAATGAAAGCTAACCTTCGGTTGACATTACAGAGCACTATCGCGTTAGAGGGACATCTCTCCCATTGCAAAATAATCGAAGCCGTAACACAAAATTCATTTCAATCAAGATTTTTTTAAGTCAATGGCTATATATATATATTCAACAGTTGACCAACGGATGATTAAAAATGAAAAGAGCATGAGAGCTCCGACCATTTCACAATGGATAAGACGTCCCTTATACGATATTGCATTCCAAGCGGTGGAGTGATGTACGTAGAAGGTTAGTTTTCATTAGTTTTTGAGGATTGGATCATTCCGCATGAATGCCGTTGGGATCGGGTCATCCGTACAAAAATCTCGTAAATTACCGTTACCCTGTTTTAGTTTTGATTGTTTATTAGAGAGGGAAGTTTTGGGTTCAAATTATGAATTCCCGTAATGTCATTTATCGTCCGAATTAGTATTGATTTTTCACTTGTTGGGCTTTCTATAAATTTTCAAGTCCATAAATGAGGAAGAGAGTTGAAAGGTTGATATAATAAGTTTAGAGTTGATAAAGTGATTTAACATAAATATGTATTTGTCTTCAATAATATGATCCATTTCTTTCTCTTGTTCTGCATCACCCTGAGGAGAAATTTCTGGTGATGTTTGTTTTGACTATGTTTTATGTTATGTTCTTCAAGAAGATAAGGTTAATTCACCCATCATTTTGAGACTGCTTTCATTGGACAGATAGACTTATTTGAATAAGATTTTCACTTGTTTCACGCAAAGAATTTTTGTCTTTCTTTATTAAATTAATTAAGATTCTTTTTTATTAGATTATGAAAAGTAAACCCATTCATTTTCCCTTAATCCTAAATAGAAACCCCATCAAGAAATTTTCATTTGTGATCACATTTTACTTGTATGACGTTCTCATATTCTGGTTTGGTTTATACTTTAGTACCTTTTATCATAATTCATTCTAAATTTTCTTTTTAGAATAAAAAAAAATTCCCCCTTTTGAAGGAAAAAGGTAGATTTATGCCATTATCTGAAAAATAACCCTGCAAATTCATATTGATTTTATTTCCAGTGTTTGAGACAAGCTGAGAAGGCTATCTCTGCTTTATTTTATTCCTTTGCTTTTTTACTTGTATCCAATTGCTGTTTAATAGCCTTTAAACAAAATTCTGTCCAACAGAAAAACTATGCTCTTTCTTGGCTCCAGAAAGAATAATGTAGGGGACAATTAATTTTCCGGTGATATCTTCAAAATATGGAATTTGGAAGTCTAGGCTACTGTTGGACCATAAGTAGTCTTCTCTGAATTGAAATCTCTGTTAATCTTGTTTAGAATGAAGGATATACTTGTAAATACTTAAATTTGATTTTATTATCTTGGAAACATTGTTTACTTAATATAGTTTTCAATATTCTTGTTAGAAATACTGACCCTTAATTGCTTTTGGAGCTAGAAGATCCAAAATTTAGGAGAAAATGAAGGGAAAAAATGGAAAGAAGATATGGTATGCCATTATGCATCCAATTCCTTAGTTTTGGGAAATAAAATTCATTTCTTTTTGTGGAGCTGTCACTTAGCTTATTCGATTTGGTCGAGATTCTTGGATGTTTTCGAACTCTCTTTCGCGTGGAGTAGAATTGCTGGCTATGATTGAGGAAATTTTGTTGAGAACTTTCCCTTTGGGGGGAAGGTGAAAATCCTTTAGTAGGTTTATCTTTTGGTTGTTATATAGATTTAGTTTGAAAGGAATCATAGAATTTTTCGAGGGGTTGAACATGCTGTTTCTTTTTTAATTCCTGTAAATTCTTTTTCGGTTTCTTATGAAAAAGTAGTTATTTCTTAATTTAATTGTAATTTCAAAGAACCCAAGATCTTACAATTCACATATTTGGCAAGTAAATGGAAAGTCCCAAGACCACAATTCCTTGAAAGTGGATAAAGAAAGCAAATAAGAAGTTGACCGTTGAAGGGGATGGTTCCCCTTTAATTCCTCTCCATTTCTCATTTACTTATTTAATGAAGCTCTGAATTTATCATTGTTAGATAACCATTCCAAATTCAAATGGATTGTTGAGAATTAACACAAGTTTACAAAGAAAAGTACCTCAAAAAGATTAAAAACAGCAGAAATAGAAGATGAGAGAAACAGAAACCACCATTGTTGGGATTAAACTGCTAATGATATTTGGTTTTGTGATGATATTTCCAACCCTGAAGGCTAATATTGCAGATTTTGATGAGGTATGGCAGAAAAGAGCTGAAGAAGCCAAGAAGGCTTCCTTTGAGGCCTACCAACCCCATCCAGAAGAAGTCACTAGTAATTTCAACAAGCAAGTTCATAAGTAATTGACCCCATTCCTCATTAGCAAATCAAGAAATTAATTTTCTGTTGAGTTGAGTTGAATATTAATAAGCATAAAACTTCATGTAGTTAATTAAAGAAGACAATATGACTATGTTGTATATATATAATTGTGGTTTTTTTTATTGTGGGACAGATCCTTAGATGGTGCAAATAACACAAGGAGACATTTGAGGAAGTACAATGGTCCATGTTTAGCGACGAATCCGATTGACCGTTGCTGGAGATGCGATAAACACTGGGCTAAAAACCGCAAGAAGCTAGCTGATTGTGCACTTGGCTTTGGTCGTGGAACAACTGGAGGCAAAAATGGAAGGATTTACGTGGTTCGAGATTCAAGTGATAACGACTTGCTGAATCCTAAACCAGGAACCCTACGCTATGGCGTGATACAGGATAGACCACTTTGGATTATATTTGCACATGACATGGTGATTAGGCTAAGTGAGGAGCTTATAATGACTGATAACAAGACATTAGACGGTCGCGGTGCCAATGTGCACATCGCTAATGGTGGTCAACTTACTCTACAATTTGTGAAGAATATAATCATCCATAATCTTCACATTCATGATATCAAGGCTGGCAATGGAGGCATTATAAGAGACTCGGTGAACCACTATGGATTCCGAACACCGAGCGATGGCGACGGAATCTCGATGTTCGGAGCCTCAAAGGTTTGGATAGACCATGTTTCAATGTCTAATTGTCAAGATGGACTAATAGATGCAGTCATGGCATCTACTGGCATTACCATTTCCAACTGTCACTTCACCCGCCATAATGATGTAAGCTCCTCAAACCATAAAAGAAAGATAACATAGCCATCGATTTATTTCATTTTTAGTTAAAATTTAACGAGTGAAGTTCAAGAGATTTGAACATCTAACTTCTTGGTCGATGACGTATGTTTTAACCAATTGAGTTATGTTCAGGTTGACAACATATTGAATTTCTTCTATATCAATGGTGATAACTAATAAATCACCATTAAATTTCTTTTGTGGATGAAGGTGATTTTATTGGGTGCGAGTGATAGCTACTCAAATGATCAGATAATGCAGGTAACTCTTGCATTTAATCACTTTGGTAAAGGGTTGGTGCAAAGGATGCCGAGATGCCGATGGGGGTTCATCCATGTAGTCAATAATGACTACACTCACTGGCTTATGTATGCCATCGGCGGCAGCCACAACCCCACCATTATCAGCCAGGGAAACCGATTCATTGCCCCACCAAACCCAAATTCCAAAGAGGTATGAAGAAGACGATTAGACAAATATCACTTATAATCACAAGCTAGATCCATTCCTAAAGAGCTTGCTCTTTATTGTGCTTCCTGAGTCTTAACCAATGAAACCAACCTCAGGATCATTTACAAAACACAAAGAAACAATATCTCCCTATTTACATATGTCCAAATGATTAGCAATGTATTGAGCAATTGTTATTGTTGGTTGATCTAGGTGACGAAAAGGGCGTATGCATCGGAAAGTGAATGGAGATCTTGGAACTGGAGGTCAGAGGGAGATCTGATGTTGAATGGAGCTTTTTTCATCCAATCTGGTAACCCCATCAGAAGATACTCCAAGAAAGATGTTATCCATTCAAAACCCGGGACCTTTGTCACCAGGCTAACACGATTCGCTGGCCCTCTGAAATGCATAAAAAACCAGCCCTGTTAGTGAAATTGTTTACTTGGCCATGTCATGGTATGTCTATAAACTACTCAAAGTTCAACCGAACCCTGTAAGCACCAGAGGTTTATAGTAGTAAAGATTTAGATAGAAGATCTACAGAACTATGACAGCTTTTGCCGTTATGAAGTTTTGTGAATCACTTGTGTGATATAATGTATTGAAATAATATTGGTTATCCATCTTTTGAAAGTTGTCTCTTTTCCTTA

mRNA sequence

AACTCTCATTTAAATGATCTTTTGAGTAAAACCATTTAGTTTTTTTGCTCTCATACATTCAACTGTTTAAAACTACATATCAATATATCATAGCACAACACAATTTCGAACAATTTATGCTTGTAATGCACCTACTAGATGCTCCTTCACGATGGCACGTGAGATTCCCCCTCCCCCTTTCCCCAAAAAAAGCAACCCACAAAAGTTCATTCCAAACGAAAACAACAAGCTCTCCCCTTTCCCCCAAAATCACTTCCATTACTCTACTCATCACTGACTCATCATTTCTTTCTTTCTCCTCCTCTACCTACTTCTTCACAGATCTTCAATGGAGATCCCAATATTCAGCAGGAGCTTTACAATGGATGAAAAAGAGGGTCTTCACGGTACCAATTTCTCGCTGCAATCGCTTAGTTACTGTGAACTATGGCCAAGGGCCTGTTTTTGCCCTTGTGGAAGAAGCTTCTCAATCCCAATGTCTTATTCTGCACTTTCTGAATGGGTGGACTTTGTTGCTAGAGAATCTGATCCCCTCATCTCATAAAAAATCCTTAGATGGTGCAAATAACACAAGGAGACATTTGAGGAAGTACAATGGTCCATGTTTAGCGACGAATCCGATTGACCGTTGCTGGAGATGCGATAAACACTGGGCTAAAAACCGCAAGAAGCTAGCTGATTGTGCACTTGGCTTTGGTCGTGGAACAACTGGAGGCAAAAATGGAAGGATTTACGTGGTTCGAGATTCAAGTGATAACGACTTGCTGAATCCTAAACCAGGAACCCTACGCTATGGCGTGATACAGGATAGACCACTTTGGATTATATTTGCACATGACATGGTGATTAGGCTAAGTGAGGAGCTTATAATGACTGATAACAAGACATTAGACGGTCGCGGTGCCAATGTGCACATCGCTAATGGTGGTCAACTTACTCTACAATTTGTGAAGAATATAATCATCCATAATCTTCACATTCATGATATCAAGGCTGGCAATGGAGGCATTATAAGAGACTCGGTGAACCACTATGGATTCCGAACACCGAGCGATGGCGACGGAATCTCGATGTTCGGAGCCTCAAAGGTTTGGATAGACCATGTTTCAATGTCTAATTGTCAAGATGGACTAATAGATGCAGTCATGGCATCTACTGGCATTACCATTTCCAACTGTCACTTCACCCGCCATAATGATGTGATTTTATTGGGTGCGAGTGATAGCTACTCAAATGATCAGATAATGCAGGTAACTCTTGCATTTAATCACTTTGGTAAAGGGTTGGTGCAAAGGATGCCGAGATGCCGATGGGGGTTCATCCATGTAGTCAATAATGACTACACTCACTGGCTTATGTATGCCATCGGCGGCAGCCACAACCCCACCATTATCAGCCAGGGAAACCGATTCATTGCCCCACCAAACCCAAATTCCAAAGAGGTGACGAAAAGGGCGTATGCATCGGAAAGTGAATGGAGATCTTGGAACTGGAGGTCAGAGGGAGATCTGATGTTGAATGGAGCTTTTTTCATCCAATCTGGTAACCCCATCAGAAGATACTCCAAGAAAGATGTTATCCATTCAAAACCCGGGACCTTTGTCACCAGGCTAACACGATTCGCTGGCCCTCTGAAATGCATAAAAAACCAGCCCTGTTAGTGAAATTGTTTACTTGGCCATGTCATGGTATGTCTATAAACTACTCAAAGTTCAACCGAACCCTGTAAGCACCAGAGGTTTATAGTAGTAAAGATTTAGATAGAAGATCTACAGAACTATGACAGCTTTTGCCGTTATGAAGTTTTGTGAATCACTTGTGTGATATAATGTATTGAAATAATATTGGTTATCCATCTTTTGAAAGTTGTCTCTTTTCCTTA

Coding sequence (CDS)

ATGCTTGTAATGCACCTACTAGATGCTCCTTCACGATGGCACGTGAGATTCCCCCTCCCCCTTTCCCCAAAAAAAGCAACCCACAAAAGTTCATTCCAAACGAAAACAACAAGCTCTCCCCTTTCCCCCAAAATCACTTCCATTACTCTACTCATCACTGACTCATCATTTCTTTCTTTCTCCTCCTCTACCTACTTCTTCACAGATCTTCAATGGAGATCCCAATATTCAGCAGGAGCTTTACAATGGATGAAAAAGAGGGTCTTCACGGTACCAATTTCTCGCTGCAATCGCTTAGTTACTGTGAACTATGGCCAAGGGCCTGTTTTTGCCCTTGTGGAAGAAGCTTCTCAATCCCAATGTCTTATTCTGCACTTTCTGAATGGGTGGACTTTGTTGCTAGAGAATCTGATCCCCTCATCTCATAAAAAATCCTTAGATGGTGCAAATAACACAAGGAGACATTTGAGGAAGTACAATGGTCCATGTTTAGCGACGAATCCGATTGACCGTTGCTGGAGATGCGATAAACACTGGGCTAAAAACCGCAAGAAGCTAGCTGATTGTGCACTTGGCTTTGGTCGTGGAACAACTGGAGGCAAAAATGGAAGGATTTACGTGGTTCGAGATTCAAGTGATAACGACTTGCTGAATCCTAAACCAGGAACCCTACGCTATGGCGTGATACAGGATAGACCACTTTGGATTATATTTGCACATGACATGGTGATTAGGCTAAGTGAGGAGCTTATAATGACTGATAACAAGACATTAGACGGTCGCGGTGCCAATGTGCACATCGCTAATGGTGGTCAACTTACTCTACAATTTGTGAAGAATATAATCATCCATAATCTTCACATTCATGATATCAAGGCTGGCAATGGAGGCATTATAAGAGACTCGGTGAACCACTATGGATTCCGAACACCGAGCGATGGCGACGGAATCTCGATGTTCGGAGCCTCAAAGGTTTGGATAGACCATGTTTCAATGTCTAATTGTCAAGATGGACTAATAGATGCAGTCATGGCATCTACTGGCATTACCATTTCCAACTGTCACTTCACCCGCCATAATGATGTGATTTTATTGGGTGCGAGTGATAGCTACTCAAATGATCAGATAATGCAGGTAACTCTTGCATTTAATCACTTTGGTAAAGGGTTGGTGCAAAGGATGCCGAGATGCCGATGGGGGTTCATCCATGTAGTCAATAATGACTACACTCACTGGCTTATGTATGCCATCGGCGGCAGCCACAACCCCACCATTATCAGCCAGGGAAACCGATTCATTGCCCCACCAAACCCAAATTCCAAAGAGGTGACGAAAAGGGCGTATGCATCGGAAAGTGAATGGAGATCTTGGAACTGGAGGTCAGAGGGAGATCTGATGTTGAATGGAGCTTTTTTCATCCAATCTGGTAACCCCATCAGAAGATACTCCAAGAAAGATGTTATCCATTCAAAACCCGGGACCTTTGTCACCAGGCTAACACGATTCGCTGGCCCTCTGAAATGCATAAAAAACCAGCCCTGTTAG

Protein sequence

MLVMHLLDAPSRWHVRFPLPLSPKKATHKSSFQTKTTSSPLSPKITSITLLITDSSFLSFSSSTYFFTDLQWRSQYSAGALQWMKKRVFTVPISRCNRLVTVNYGQGPVFALVEEASQSQCLILHFLNGWTLLLENLIPSSHKKSLDGANNTRRHLRKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGGKNGRIYVVRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDGDGISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSNDQIMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRAYASESEWRSWNWRSEGDLMLNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGPLKCIKNQPC
Homology
BLAST of Bhi08M001165 vs. TAIR 10
Match: AT5G15110.1 (Pectate lyase family protein )

HSP 1 Score: 509.6 bits (1311), Expect = 2.9e-144
Identity = 231/376 (61.44%), Postives = 293/376 (77.93%), Query Frame = 0

Query: 150 NNTRRHLR--------KYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGGK 209
           N+TRR LR        K  GPC A+NPID+CWRC   WAK RKKL  C  GFG  TTGGK
Sbjct: 97  NSTRRSLRGKGKGKWSKLKGPCTASNPIDKCWRCRSDWAKRRKKLTRCVRGFGHRTTGGK 156

Query: 210 NGRIYVVRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTDNKTLDGR 269
            GRIYVV  + D D++NPKPGTLR+ VIQ  PLWIIF +DM IRL++EL++  +KT+D R
Sbjct: 157 RGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEPLWIIFKNDMSIRLNQELLINSHKTIDAR 216

Query: 270 GANVHIANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDGDGISMFG 329
           GANVH+A+G  +T+QFVKN++IH LHIH I   +GG+IRDSV+H+G RT +DGDG+S++G
Sbjct: 217 GANVHVAHGAGITMQFVKNVVIHGLHIHHISESSGGMIRDSVDHFGMRTRADGDGLSIYG 276

Query: 330 ASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSNDQIMQVTL 389
           +S +W+DH+SMS CQDGLIDA++ STGITISN HFT HNDV+LLGA ++   D+ MQVT+
Sbjct: 277 SSNIWLDHISMSKCQDGLIDAIVGSTGITISNSHFTHHNDVMLLGAQNTNEADKHMQVTV 336

Query: 390 AFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPN-PNS 449
           A+NHFGKGLVQRMPR RWGF+HVVNNDYTHW +YAIGGS  PTI+S GNRFIAPP+ P+ 
Sbjct: 337 AYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWELYAIGGSQGPTILSHGNRFIAPPHKPHY 396

Query: 450 KEVTKRAYASESEWRSWNWRSEGDLMLNGAFFIQSGNPIRR--YSKKDVIHSKPGTFVTR 509
           +EVTKR YASE EW+ WNWRS+ D+ +NGA+F QSGNP  +  ++++ +I  K G  V++
Sbjct: 397 REVTKRDYASEDEWKHWNWRSDKDVFMNGAYFRQSGNPQYKCAHTRQQMIKPKNGLAVSK 456

Query: 510 LTRFAGPLKCIKNQPC 515
           LT++AG L C   + C
Sbjct: 457 LTKYAGALDCRVGRRC 472

BLAST of Bhi08M001165 vs. TAIR 10
Match: AT1G14420.1 (Pectate lyase family protein )

HSP 1 Score: 504.2 bits (1297), Expect = 1.2e-142
Identity = 236/384 (61.46%), Postives = 284/384 (73.96%), Query Frame = 0

Query: 147 DGANNTRRHL---------RKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGT 206
           +  N+TRR L          K +G CLA NPID CWRCD++WA NRKKLADC LGFGR T
Sbjct: 76  ESRNDTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRT 135

Query: 207 TGGKNGRIYVVRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTDNKT 266
           TGGK+G IYVV+D+SDNDL+NPKPGTLR+ V +D PLWIIFA  M+I+L +EL++T +KT
Sbjct: 136 TGGKDGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKT 195

Query: 267 LDGRGANVHIANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDGDGI 326
           +DGRGA V+I  G  LTLQFV N+IIHN+++  I  GNGG+IRDS  H G RT SDGDGI
Sbjct: 196 IDGRGARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGI 255

Query: 327 SMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSNDQIM 386
           S+FGA+ +WIDHVSM+ C DG+IDA+  ST +TISN HFT H +V+L GA D +  D+ M
Sbjct: 256 SLFGATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKM 315

Query: 387 QVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPN 446
           Q+T+AFNHFGK L QRMPRCR+G IHVVNNDYTHW MYAIGG+ NPTIISQGNRFIAPPN
Sbjct: 316 QITVAFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPN 375

Query: 447 PNSKEVTKRAYASESEWRSWNWRSEGDLMLNGAFFIQSG-------NPIRRYSKKDVIHS 506
             +K++TKR Y    EW+SWNW+SEGD  LNGA+F+QSG        P      K  I  
Sbjct: 376 EEAKQITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRP 435

Query: 507 KPGTFVTRLTRFAGPLKCIKNQPC 515
           KPGT V +LT  AG L C   + C
Sbjct: 436 KPGTMVRKLTMDAGVLGCKLGEAC 459

BLAST of Bhi08M001165 vs. TAIR 10
Match: AT3G01270.1 (Pectate lyase family protein )

HSP 1 Score: 504.2 bits (1297), Expect = 1.2e-142
Identity = 232/380 (61.05%), Postives = 290/380 (76.32%), Query Frame = 0

Query: 150 NNTRRHL------------RKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGT 209
           N+TRR L             K  GPC A+NPID+CWRC   WA+ RKKL  C  GFG  T
Sbjct: 96  NSTRRSLTGRGKGKGKGKWSKLTGPCTASNPIDKCWRCQPDWARRRKKLVHCVRGFGYRT 155

Query: 210 TGGKNGRIYVVRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTDNKT 269
           TGGK GRIYVV    D+D++NP+PGTLR+ VIQ  PLWI+F HDM IRLS+EL++T +KT
Sbjct: 156 TGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQKEPLWIVFKHDMSIRLSQELMITSDKT 215

Query: 270 LDGRGANVHIANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDGDGI 329
           +D RGANVHIA G  +T+Q+V NIIIH LH+H I   +GG+IRDS+NH+G R  +DGDGI
Sbjct: 216 IDARGANVHIAYGAGITMQYVHNIIIHGLHVHHIVKSSGGLIRDSINHFGHRGEADGDGI 275

Query: 330 SMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSNDQIM 389
           S+FGA+ +W+DH+SMS CQDGLIDA+M ST ITISN HFT HNDV+LLGA ++  +D+ M
Sbjct: 276 SIFGATNIWLDHISMSKCQDGLIDAIMGSTAITISNSHFTHHNDVMLLGAQNNNMDDKKM 335

Query: 390 QVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPN 449
           QVT+A+NHFGKGLVQRMPR RWGF+HVVNNDYTHW +YAIGGS  PTI+S GNRFIAPP+
Sbjct: 336 QVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYTHWELYAIGGSQGPTILSHGNRFIAPPH 395

Query: 450 -PNSKEVTKRAYASESEWRSWNWRSEGDLMLNGAFFIQSGNPIRR--YSKKDVIHSKPGT 509
             + +EVTKR YASESEW++WNWRSE D+ +N A+F QSGNP  +  +S++ +I  K G 
Sbjct: 396 KQHYREVTKRDYASESEWKNWNWRSEKDVFMNNAYFRQSGNPHFKCSHSRQQMIKPKNGM 455

Query: 510 FVTRLTRFAGPLKCIKNQPC 515
            V++LT++AG L C   + C
Sbjct: 456 AVSKLTKYAGALDCRVGKAC 475

BLAST of Bhi08M001165 vs. TAIR 10
Match: AT2G02720.1 (Pectate lyase family protein )

HSP 1 Score: 484.6 bits (1246), Expect = 1.0e-136
Identity = 235/384 (61.20%), Postives = 283/384 (73.70%), Query Frame = 0

Query: 150 NNTRRHLR---------KYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGG 209
           N TRR LR         +  G   + N ID+CWR DK+W KNRKKLADC LGFGR TTGG
Sbjct: 75  NETRRDLRQVGRGKKTTRRGGRFESLNAIDKCWRGDKNWDKNRKKLADCVLGFGRKTTGG 134

Query: 210 KNGRIYVVRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTDNKTLDG 269
           KNG IYVV D SDNDLL PKPGT+R+ V +DRPLWIIFA  M+I+L +ELI+T++KT+DG
Sbjct: 135 KNGPIYVVTDPSDNDLLKPKPGTIRHAVTRDRPLWIIFARSMIIKLQQELIITNDKTIDG 194

Query: 270 RGANVHIANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDGDGISMF 329
           RGA ++I  G  LTLQFV+N+IIHN+HI  IK G GG+I DS  H+G RT SDGDGI++F
Sbjct: 195 RGAKIYITGGAGLTLQFVRNVIIHNIHIKQIKRGAGGLIIDSEQHFGLRTVSDGDGINIF 254

Query: 330 GASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSNDQIMQVT 389
           GA+ VWIDHVSM++C DG+IDA+M ST ITISN HFT H++V+L G ++    D+ MQ+T
Sbjct: 255 GATNVWIDHVSMTDCSDGMIDAIMGSTAITISNSHFTDHDEVMLFGGTNKDVIDKKMQIT 314

Query: 390 LAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNS 449
           +AFNHFGK L QRMPR R+G +HVVNNDYTHW MYAIGG+ NPTIISQGNRFIAPP  +S
Sbjct: 315 VAFNHFGKRLKQRMPRVRFGLVHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPIEDS 374

Query: 450 KEVTKRAYASESEWRSWNWRSEGDLMLNGAFFIQSG----------NPIRRYSKKDVIHS 509
           K+VTKR Y    EW+SWNW+SE D  LNGA+F+QSG          NPI R   K  I  
Sbjct: 375 KQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPR---KFAIRP 434

Query: 510 KPGTFVTRLTRFAGPLKCIKNQPC 515
           +PGT V RLT+ AG L C   + C
Sbjct: 435 QPGTKVRRLTKDAGTLGCKPGKSC 455

BLAST of Bhi08M001165 vs. TAIR 10
Match: AT5G63180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 484.2 bits (1245), Expect = 1.3e-136
Identity = 230/383 (60.05%), Postives = 280/383 (73.11%), Query Frame = 0

Query: 135 ENLIPSSHKKSLDGANNTRRHLRKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFG 194
           E ++   H+K  +    +RR L  ++  C + NPID CWRCDK W KNRK+LADC +GFG
Sbjct: 54  ELVVEEVHRKINESI--SRRKLGFFS--CGSGNPIDDCWRCDKDWEKNRKRLADCGIGFG 113

Query: 195 RGTTGGKNGRIYVVRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTD 254
           +   GG++G IYVV D  ++D +NP+PGTLRY VIQD PLWIIF  DM I+L EELIM  
Sbjct: 114 KNAIGGRDGEIYVVTDPGNDDPVNPRPGTLRYAVIQDEPLWIIFKRDMTIQLKEELIMNS 173

Query: 255 NKTLDGRGANVHIANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDG 314
            KTLDGRGA+VHI+ G  +T+Q+V NIIIH LHIHD K G    +RDS  HYG+RT SDG
Sbjct: 174 FKTLDGRGASVHISGGPCITIQYVTNIIIHGLHIHDCKQGGNTYVRDSPEHYGYRTVSDG 233

Query: 315 DGISMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSND 374
           DG+S+FG S VW+DH S+SNC DGLIDA+  ST ITISN + T HN V+LLG SD+Y  D
Sbjct: 234 DGVSIFGGSHVWVDHCSLSNCNDGLIDAIRGSTAITISNNYLTHHNKVMLLGHSDTYEQD 293

Query: 375 QIMQVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIA 434
           + MQVT+AFNHFG+GLVQRMPRCR G+ HVVNNDYTHW MYAIGGS NPTI SQGNRF+A
Sbjct: 294 KNMQVTIAFNHFGEGLVQRMPRCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQGNRFLA 353

Query: 435 PPNPNSKEVTKRAYASESEWRSWNWRSEGDLMLNGAFFIQSG-NPIR--RYSKKDVIHSK 494
           P + +SKEVTK   A E EWR+WNWRSEGDL+LNGAFF  SG  P +   YSK   + ++
Sbjct: 354 PDDSSSKEVTKHEDAPEDEWRNWNWRSEGDLLLNGAFFTYSGAGPAKSSSYSKASSLAAR 413

Query: 495 PGTFVTRLTRFAGPLKCIKNQPC 515
           P + V  +T  +G L C +   C
Sbjct: 414 PSSHVGEITIASGALSCKRGSHC 432

BLAST of Bhi08M001165 vs. ExPASy Swiss-Prot
Match: P40973 (Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 1.3e-152
Identity = 247/367 (67.30%), Postives = 296/367 (80.65%), Query Frame = 0

Query: 150 NNTRRHLRKYN-GPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGGKNGRIYVV 209
           N+TRR+LR    G CLATNPIDRCWRC K+W+ NRK L  C  GFGR TTGG  G IYVV
Sbjct: 68  NSTRRNLRTNKLGQCLATNPIDRCWRCKKNWSANRKDLVKCVKGFGRKTTGGAAGEIYVV 127

Query: 210 RDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHIA 269
            D SD+ L +PK GTLR+GVIQDRPLWIIF   MVIRL +ELI+ ++KT+DGRGANV IA
Sbjct: 128 TDPSDDSLTDPKFGTLRWGVIQDRPLWIIFGKSMVIRLKQELIINNDKTIDGRGANVQIA 187

Query: 270 NGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDGDGISMFGASKVWID 329
            G QLT+QFV N+IIH +HIHDIK G GG+IRDS  H G RT SDGDGIS+ G+S +WID
Sbjct: 188 GGAQLTVQFVHNVIIHGIHIHDIKPGEGGLIRDSEKHSGIRTRSDGDGISIIGSSNIWID 247

Query: 330 HVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSNDQIMQVTLAFNHFGK 389
           HVS++ C DGLID ++ ST ITISNCH T H+DV+LLGASD+Y+ D+IMQVT+AFNHFG+
Sbjct: 248 HVSLARCSDGLIDVILGSTAITISNCHLTEHDDVMLLGASDTYTQDEIMQVTVAFNHFGR 307

Query: 390 GLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRAY 449
           GLVQRMPRCR+GF+HVVNNDYTHW+MYA+GGS +PTIISQGNR+IAP    +KEVTKR Y
Sbjct: 308 GLVQRMPRCRYGFVHVVNNDYTHWIMYAVGGSQHPTIISQGNRYIAPHIEAAKEVTKRDY 367

Query: 450 ASESEWRSWNWRSEGDLMLNGAFFIQSGNPI-RRYSKKDVIHSKPGTFVTRLTRFAGPLK 509
           A  +EW  W W+S+GDL ++GAFF++SG P   +YSKKD+I +KPGTFV RLTRF+G L 
Sbjct: 368 AEPAEWSKWTWKSQGDLFVSGAFFVESGGPFENKYSKKDLIKAKPGTFVQRLTRFSGALN 427

Query: 510 CIKNQPC 515
           C +N  C
Sbjct: 428 CKENMEC 434

BLAST of Bhi08M001165 vs. ExPASy Swiss-Prot
Match: P15722 (Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 2.2e-144
Identity = 242/370 (65.41%), Postives = 284/370 (76.76%), Query Frame = 0

Query: 150 NNTRRHL--RKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGGKNGRIYV 209
           N+TRR L  +KY GPC+ TNPID+CWRCD +WA NRKKLADCA+GFG    GGK+G  YV
Sbjct: 81  NSTRRGLGTKKYTGPCMVTNPIDKCWRCDPNWADNRKKLADCAMGFGSKAIGGKDGEFYV 140

Query: 210 VRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANVHI 269
           V D+SD D  +PKPGTLR+ VIQ  PLWIIF   M IRL +E+IM  +KT+D RG NVHI
Sbjct: 141 VTDNSD-DYNDPKPGTLRHAVIQKEPLWIIFKRGMNIRLHQEMIMQSDKTIDARGVNVHI 200

Query: 270 ANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDGDGISMFGASKVWI 329
             G  +TLQ++KN+IIH LHIHDI  GNGG++RD+V+H G RT SDGDGIS+FGAS +WI
Sbjct: 201 TKGAGITLQYIKNVIIHGLHIHDIVEGNGGMVRDAVDHIGIRTKSDGDGISIFGASYIWI 260

Query: 330 DHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSNDQIMQVTLAFNHFG 389
           DHVSM  C DGLIDAV  STGITISN HFT HN+V+L GASDS S DQ+MQ+TLAFNHFG
Sbjct: 261 DHVSMQRCYDGLIDAVEGSTGITISNGHFTDHNEVMLFGASDSSSIDQVMQITLAFNHFG 320

Query: 390 KGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTKRA 449
           K L+QRMPRCRWG+IHVVNNDYTHW MYAIGGS +PTII QGNRFIAPP+   K+VTKR 
Sbjct: 321 KRLIQRMPRCRWGYIHVVNNDYTHWNMYAIGGSMHPTIIHQGNRFIAPPDIFKKQVTKRE 380

Query: 450 YASESEWRSWNWRSEGDLMLNGAFFIQSGNPIRRYSKKDV---IHSKPGTFVTRLTRFAG 509
           Y  ES W  W WRSEG+L +NGA+F +SG+P      KD+   I + P   VT +TRFAG
Sbjct: 381 YNPESVWMQWTWRSEGNLFMNGAYFTESGDPEWSSKHKDLYDGISAAPAEDVTWMTRFAG 440

Query: 510 PLKCIKNQPC 515
            L C   +PC
Sbjct: 441 VLGCKPGKPC 449

BLAST of Bhi08M001165 vs. ExPASy Swiss-Prot
Match: Q9LFP5 (Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 3.1e-143
Identity = 232/376 (61.70%), Postives = 293/376 (77.93%), Query Frame = 0

Query: 150 NNTRRHLR--------KYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGGK 209
           N+TRR LR        K  GPC A+NPID+CWRC   WAK RKKL  C  GFG  TTGGK
Sbjct: 97  NSTRRSLRGKGKGKWSKLKGPCTASNPIDKCWRCRSDWAKRRKKLTRCVRGFGHRTTGGK 156

Query: 210 NGRIYVVRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTDNKTLDGR 269
            GRIYVV  + D D++NPKPGTLR+ VIQ  PLWIIF +DM IRL++EL++  +KT+D R
Sbjct: 157 RGRIYVVTSNLDEDMVNPKPGTLRHAVIQKEPLWIIFKNDMSIRLNQELLINSHKTIDAR 216

Query: 270 GANVHIANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDGDGISMFG 329
           GANVH+A+G  +T+QFVKN+IIH LHIH I   +GG+IRDSV+H+G RT +DGDG+S++G
Sbjct: 217 GANVHVAHGAGITMQFVKNVIIHGLHIHHISESSGGMIRDSVDHFGMRTRADGDGLSIYG 276

Query: 330 ASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSNDQIMQVTL 389
           +S +W+DH+SMS CQDGLIDA++ STGITISN HFT HNDV+LLGA ++   D+ MQVT+
Sbjct: 277 SSNIWLDHISMSKCQDGLIDAIVGSTGITISNSHFTHHNDVMLLGAQNTNEADKHMQVTV 336

Query: 390 AFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPN-PNS 449
           A+NHFGKGLVQRMPR RWGF+HVVNNDYTHW +YAIGGS  PTI+S GNRFIAPP+ P+ 
Sbjct: 337 AYNHFGKGLVQRMPRIRWGFVHVVNNDYTHWELYAIGGSQGPTILSHGNRFIAPPHKPHY 396

Query: 450 KEVTKRAYASESEWRSWNWRSEGDLMLNGAFFIQSGNPIRR--YSKKDVIHSKPGTFVTR 509
           +EVTKR YASE EW+ WNWRS+ D+ +NGA+F QSGNP  +  ++++ +I  K G  V++
Sbjct: 397 REVTKRDYASEDEWKHWNWRSDKDVFMNGAYFRQSGNPQYKCAHTRQQMIKPKNGLAVSK 456

Query: 510 LTRFAGPLKCIKNQPC 515
           LT++AG L C   + C
Sbjct: 457 LTKYAGALDCRVGRRC 472

BLAST of Bhi08M001165 vs. ExPASy Swiss-Prot
Match: Q9M9S2 (Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.7e-141
Identity = 236/384 (61.46%), Postives = 284/384 (73.96%), Query Frame = 0

Query: 147 DGANNTRRHL---------RKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGT 206
           +  N+TRR L          K +G CLA NPID CWRCD++WA NRKKLADC LGFGR T
Sbjct: 76  ESRNDTRRELTQVRSGRKTHKSSGKCLAYNPIDNCWRCDRNWANNRKKLADCVLGFGRRT 135

Query: 207 TGGKNGRIYVVRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTDNKT 266
           TGGK+G IYVV+D+SDNDL+NPKPGTLR+ V +D PLWIIFA  M+I+L +EL++T +KT
Sbjct: 136 TGGKDGPIYVVKDASDNDLINPKPGTLRHAVTRDGPLWIIFARSMIIKLQQELMITSDKT 195

Query: 267 LDGRGANVHIANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDGDGI 326
           +DGRGA V+I  G  LTLQFV N+IIHN+++  I  GNGG+IRDS  H G RT SDGDGI
Sbjct: 196 IDGRGARVYIMEGAGLTLQFVNNVIIHNIYVKHIVPGNGGLIRDSEAHIGLRTKSDGDGI 255

Query: 327 SMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSNDQIM 386
           S+FGA+ +WIDHVSM+ C DG+IDA+  ST +TISN HFT H +V+L GA D +  D+ M
Sbjct: 256 SLFGATNIWIDHVSMTRCADGMIDAIDGSTAVTISNSHFTDHQEVMLFGARDEHVIDKKM 315

Query: 387 QVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPN 446
           Q+T+AFNHFGK L QRMPRCR+G IHVVNNDYTHW MYAIGG+ NPTIISQGNRFIAPPN
Sbjct: 316 QITVAFNHFGKRLEQRMPRCRYGTIHVVNNDYTHWEMYAIGGNMNPTIISQGNRFIAPPN 375

Query: 447 PNSKEVTKRAYASESEWRSWNWRSEGDLMLNGAFFIQSG-------NPIRRYSKKDVIHS 506
             +K++TKR Y    EW+SWNW+SEGD  LNGA+F+QSG        P      K  I  
Sbjct: 376 EEAKQITKREYTPYGEWKSWNWQSEGDYFLNGAYFVQSGKANAWSSKPKTPLPNKFTIRP 435

Query: 507 KPGTFVTRLTRFAGPLKCIKNQPC 515
           KPGT V +LT  AG L C   + C
Sbjct: 436 KPGTMVRKLTMDAGVLGCKLGEAC 459

BLAST of Bhi08M001165 vs. ExPASy Swiss-Prot
Match: Q9SRH4 (Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.7e-141
Identity = 232/380 (61.05%), Postives = 290/380 (76.32%), Query Frame = 0

Query: 150 NNTRRHL------------RKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGT 209
           N+TRR L             K  GPC A+NPID+CWRC   WA+ RKKL  C  GFG  T
Sbjct: 96  NSTRRSLTGRGKGKGKGKWSKLTGPCTASNPIDKCWRCQPDWARRRKKLVHCVRGFGYRT 155

Query: 210 TGGKNGRIYVVRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTDNKT 269
           TGGK GRIYVV    D+D++NP+PGTLR+ VIQ  PLWI+F HDM IRLS+EL++T +KT
Sbjct: 156 TGGKRGRIYVVTSPRDDDMVNPRPGTLRHAVIQKEPLWIVFKHDMSIRLSQELMITSDKT 215

Query: 270 LDGRGANVHIANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDGDGI 329
           +D RGANVHIA G  +T+Q+V NIIIH LH+H I   +GG+IRDS+NH+G R  +DGDGI
Sbjct: 216 IDARGANVHIAYGAGITMQYVHNIIIHGLHVHHIVKSSGGLIRDSINHFGHRGEADGDGI 275

Query: 330 SMFGASKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSNDQIM 389
           S+FGA+ +W+DH+SMS CQDGLIDA+M ST ITISN HFT HNDV+LLGA ++  +D+ M
Sbjct: 276 SIFGATNIWLDHISMSKCQDGLIDAIMGSTAITISNSHFTHHNDVMLLGAQNNNMDDKKM 335

Query: 390 QVTLAFNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPN 449
           QVT+A+NHFGKGLVQRMPR RWGF+HVVNNDYTHW +YAIGGS  PTI+S GNRFIAPP+
Sbjct: 336 QVTVAYNHFGKGLVQRMPRVRWGFVHVVNNDYTHWELYAIGGSQGPTILSHGNRFIAPPH 395

Query: 450 -PNSKEVTKRAYASESEWRSWNWRSEGDLMLNGAFFIQSGNPIRR--YSKKDVIHSKPGT 509
             + +EVTKR YASESEW++WNWRSE D+ +N A+F QSGNP  +  +S++ +I  K G 
Sbjct: 396 KQHYREVTKRDYASESEWKNWNWRSEKDVFMNNAYFRQSGNPHFKCSHSRQQMIKPKNGM 455

Query: 510 FVTRLTRFAGPLKCIKNQPC 515
            V++LT++AG L C   + C
Sbjct: 456 AVSKLTKYAGALDCRVGKAC 475

BLAST of Bhi08M001165 vs. ExPASy TrEMBL
Match: A0A5D3BH70 (Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003070 PE=3 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 7.4e-212
Identity = 344/371 (92.72%), Postives = 361/371 (97.30%), Query Frame = 0

Query: 144 KSLDGANNTRRHLRKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGGKNG 203
           +SLDGANNTRRHLRKY GPCLATNPIDRCWRCD++WA+NRKKLADCALGFGRGTTGGK+G
Sbjct: 69  RSLDGANNTRRHLRKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDG 128

Query: 204 RIYVVRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTDNKTLDGRGA 263
           +IYVVRDSSDNDL+NPKPGTLR+ VIQ+RPLWIIFAHDMVIRLSEELIMTDNKTLDGRGA
Sbjct: 129 KIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGA 188

Query: 264 NVHIANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDGDGISMFGAS 323
           NVHIANGGQ+TLQFV+NIIIHNLHIHDIKAGNGG IRDSV+HYG+RT SDGDGISMFG+S
Sbjct: 189 NVHIANGGQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSS 248

Query: 324 KVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSNDQIMQVTLAF 383
           +VWIDHVSMSNCQDGLIDAVMAST ITISNCHFT HNDVILLGASDSYSND+IMQVTLAF
Sbjct: 249 RVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAF 308

Query: 384 NHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEV 443
           NHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEV
Sbjct: 309 NHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEV 368

Query: 444 TKRAYASESEWRSWNWRSEGDLMLNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFA 503
           TKR YASE EWRSWNWRSEGDLM+NGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFA
Sbjct: 369 TKRVYASEREWRSWNWRSEGDLMMNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFA 428

Query: 504 GPLKCIKNQPC 515
           GPLKC  NQPC
Sbjct: 429 GPLKCKMNQPC 439

BLAST of Bhi08M001165 vs. ExPASy TrEMBL
Match: A0A1S3BF07 (Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103488907 PE=3 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 7.4e-212
Identity = 344/371 (92.72%), Postives = 361/371 (97.30%), Query Frame = 0

Query: 144 KSLDGANNTRRHLRKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGGKNG 203
           +SLDGANNTRRHLRKY GPCLATNPIDRCWRCD++WA+NRKKLADCALGFGRGTTGGK+G
Sbjct: 30  RSLDGANNTRRHLRKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALGFGRGTTGGKDG 89

Query: 204 RIYVVRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTDNKTLDGRGA 263
           +IYVVRDSSDNDL+NPKPGTLR+ VIQ+RPLWIIFAHDMVIRLSEELIMTDNKTLDGRGA
Sbjct: 90  KIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIMTDNKTLDGRGA 149

Query: 264 NVHIANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDGDGISMFGAS 323
           NVHIANGGQ+TLQFV+NIIIHNLHIHDIKAGNGG IRDSV+HYG+RT SDGDGISMFG+S
Sbjct: 150 NVHIANGGQITLQFVRNIIIHNLHIHDIKAGNGGFIRDSVSHYGYRTQSDGDGISMFGSS 209

Query: 324 KVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSNDQIMQVTLAF 383
           +VWIDHVSMSNCQDGLIDAVMAST ITISNCHFT HNDVILLGASDSYSND+IMQVTLAF
Sbjct: 210 RVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASDSYSNDKIMQVTLAF 269

Query: 384 NHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEV 443
           NHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEV
Sbjct: 270 NHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEV 329

Query: 444 TKRAYASESEWRSWNWRSEGDLMLNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFA 503
           TKR YASE EWRSWNWRSEGDLM+NGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFA
Sbjct: 330 TKRVYASEREWRSWNWRSEGDLMMNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFA 389

Query: 504 GPLKCIKNQPC 515
           GPLKC  NQPC
Sbjct: 390 GPLKCKMNQPC 400

BLAST of Bhi08M001165 vs. ExPASy TrEMBL
Match: A0A6J1C763 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009024 PE=3 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 9.0e-210
Identity = 350/429 (81.59%), Postives = 378/429 (88.11%), Query Frame = 0

Query: 124 LHFLNGWTLLLENLIPSSHK-------------------------------------KSL 183
           LHFLNGWTLL E+  P SHK                                     KSL
Sbjct: 31  LHFLNGWTLLRES-DPPSHKNFDEVWQKRAQEAKKAAIEAYQPHPEEVTSNFNQQVHKSL 90

Query: 184 DGANNTRRHLRKYN-GPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGGKNGRI 243
           DGANNTRRHL+ Y+ GPCLATNPIDRCWRCDKHWAKNRKKLA+CALGFGRGTTGGK+GR 
Sbjct: 91  DGANNTRRHLKHYSGGPCLATNPIDRCWRCDKHWAKNRKKLAECALGFGRGTTGGKDGRY 150

Query: 244 YVVRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTDNKTLDGRGANV 303
           YVVRDSSDNDLLNPKPGTLRYGVIQ++PLWI+FAHDMVIRLSEELI+TDNKT+DGRGANV
Sbjct: 151 YVVRDSSDNDLLNPKPGTLRYGVIQEKPLWIVFAHDMVIRLSEELIITDNKTIDGRGANV 210

Query: 304 HIANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDGDGISMFGASKV 363
           HIANGGQ+TLQFVKNIII NLHIHDIK G+GG+IRDSV+HYG RTPSDGDGISMFGA+K+
Sbjct: 211 HIANGGQITLQFVKNIIISNLHIHDIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGATKI 270

Query: 364 WIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSNDQIMQVTLAFNH 423
           WIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDV+LLGASDSYSNDQIMQVTLAFNH
Sbjct: 271 WIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVMLLGASDSYSNDQIMQVTLAFNH 330

Query: 424 FGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEVTK 483
           FGKGLVQRMPRCRWGF+HVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPP+PN KEVTK
Sbjct: 331 FGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPDPNCKEVTK 390

Query: 484 RAYASESEWRSWNWRSEGDLMLNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFAGP 515
           RAYASESEW+SWNWRSEGDLM+NGAFFI+SG+PIR+YSKKDVI +KPG+FVTRLTRF+GP
Sbjct: 391 RAYASESEWKSWNWRSEGDLMMNGAFFIESGSPIRKYSKKDVITAKPGSFVTRLTRFSGP 450

BLAST of Bhi08M001165 vs. ExPASy TrEMBL
Match: A0A0A0LR32 (Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G045510 PE=3 SV=1)

HSP 1 Score: 739.2 bits (1907), Expect = 1.2e-209
Identity = 341/371 (91.91%), Postives = 359/371 (96.77%), Query Frame = 0

Query: 144 KSLDGANNTRRHLRKYNGPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGGKNG 203
           +SLDG NNTRRHLRKY GPCLATNPIDRCWRCD++WA+NRKKLADCALGFGR TTGGK+G
Sbjct: 69  RSLDGGNNTRRHLRKYTGPCLATNPIDRCWRCDRNWARNRKKLADCALGFGRRTTGGKDG 128

Query: 204 RIYVVRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTDNKTLDGRGA 263
           +IYVVRDSSDNDL+NPKPGTLR+ VIQ+RPLWIIFAHDMVIRLSEELI+TD+KTLDGRGA
Sbjct: 129 KIYVVRDSSDNDLVNPKPGTLRHAVIQERPLWIIFAHDMVIRLSEELIVTDDKTLDGRGA 188

Query: 264 NVHIANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDGDGISMFGAS 323
           NVHIANGGQ+TLQFVKNIIIHNLHIHDIKAGNGG+IRDSV+HYGFRT SDGDGISMFGAS
Sbjct: 189 NVHIANGGQITLQFVKNIIIHNLHIHDIKAGNGGMIRDSVSHYGFRTRSDGDGISMFGAS 248

Query: 324 KVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSNDQIMQVTLAF 383
           +VWIDHVSMSNCQDGLIDAVMAST ITISNCHFT HNDVILLGAS+ YSNDQIMQVTLAF
Sbjct: 249 RVWIDHVSMSNCQDGLIDAVMASTAITISNCHFTHHNDVILLGASNGYSNDQIMQVTLAF 308

Query: 384 NHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKEV 443
           NHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPN KEV
Sbjct: 309 NHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNCKEV 368

Query: 444 TKRAYASESEWRSWNWRSEGDLMLNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRFA 503
           TKR YA ESEWRSWNWRSEGDLM+NGAFFIQSGNPI+RYSKKDVIHSKPGTFVTRLTRFA
Sbjct: 369 TKRVYAPESEWRSWNWRSEGDLMMNGAFFIQSGNPIKRYSKKDVIHSKPGTFVTRLTRFA 428

Query: 504 GPLKCIKNQPC 515
           GPLKC KNQPC
Sbjct: 429 GPLKCKKNQPC 439

BLAST of Bhi08M001165 vs. ExPASy TrEMBL
Match: A0A6J1C762 (Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009024 PE=3 SV=1)

HSP 1 Score: 730.7 bits (1885), Expect = 4.2e-207
Identity = 335/372 (90.05%), Postives = 362/372 (97.31%), Query Frame = 0

Query: 144 KSLDGANNTRRHLRKYN-GPCLATNPIDRCWRCDKHWAKNRKKLADCALGFGRGTTGGKN 203
           KSLDGANNTRRHL+ Y+ GPCLATNPIDRCWRCDKHWAKNRKKLA+CALGFGRGTTGGK+
Sbjct: 46  KSLDGANNTRRHLKHYSGGPCLATNPIDRCWRCDKHWAKNRKKLAECALGFGRGTTGGKD 105

Query: 204 GRIYVVRDSSDNDLLNPKPGTLRYGVIQDRPLWIIFAHDMVIRLSEELIMTDNKTLDGRG 263
           GR YVVRDSSDNDLLNPKPGTLRYGVIQ++PLWI+FAHDMVIRLSEELI+TDNKT+DGRG
Sbjct: 106 GRYYVVRDSSDNDLLNPKPGTLRYGVIQEKPLWIVFAHDMVIRLSEELIITDNKTIDGRG 165

Query: 264 ANVHIANGGQLTLQFVKNIIIHNLHIHDIKAGNGGIIRDSVNHYGFRTPSDGDGISMFGA 323
           ANVHIANGGQ+TLQFVKNIII NLHIHDIK G+GG+IRDSV+HYG RTPSDGDGISMFGA
Sbjct: 166 ANVHIANGGQITLQFVKNIIISNLHIHDIKNGHGGLIRDSVSHYGLRTPSDGDGISMFGA 225

Query: 324 SKVWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVILLGASDSYSNDQIMQVTLA 383
           +K+WIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDV+LLGASDSYSNDQIMQVTLA
Sbjct: 226 TKIWIDHVSMSNCQDGLIDAVMASTGITISNCHFTRHNDVMLLGASDSYSNDQIMQVTLA 285

Query: 384 FNHFGKGLVQRMPRCRWGFIHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPNPNSKE 443
           FNHFGKGLVQRMPRCRWGF+HVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPP+PN KE
Sbjct: 286 FNHFGKGLVQRMPRCRWGFVHVVNNDYTHWLMYAIGGSHNPTIISQGNRFIAPPDPNCKE 345

Query: 444 VTKRAYASESEWRSWNWRSEGDLMLNGAFFIQSGNPIRRYSKKDVIHSKPGTFVTRLTRF 503
           VTKRAYASESEW+SWNWRSEGDLM+NGAFFI+SG+PIR+YSKKDVI +KPG+FVTRLTRF
Sbjct: 346 VTKRAYASESEWKSWNWRSEGDLMMNGAFFIESGSPIRKYSKKDVITAKPGSFVTRLTRF 405

Query: 504 AGPLKCIKNQPC 515
           +GPLKC  N+PC
Sbjct: 406 SGPLKCRNNRPC 417

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G15110.12.9e-14461.44Pectate lyase family protein [more]
AT1G14420.11.2e-14261.46Pectate lyase family protein [more]
AT3G01270.11.2e-14261.05Pectate lyase family protein [more]
AT2G02720.11.0e-13661.20Pectate lyase family protein [more]
AT5G63180.11.3e-13660.05Pectin lyase-like superfamily protein [more]
Match NameE-valueIdentityDescription
P409731.3e-15267.30Pectate lyase OS=Lilium longiflorum OX=4690 PE=2 SV=1[more]
P157222.2e-14465.41Probable pectate lyase P59 OS=Solanum lycopersicum OX=4081 GN=LAT59 PE=2 SV=1[more]
Q9LFP53.1e-14361.70Probable pectate lyase 19 OS=Arabidopsis thaliana OX=3702 GN=At5g15110 PE=2 SV=1[more]
Q9M9S21.7e-14161.46Probable pectate lyase 3 OS=Arabidopsis thaliana OX=3702 GN=AT59 PE=2 SV=1[more]
Q9SRH41.7e-14161.05Probable pectate lyase 7 OS=Arabidopsis thaliana OX=3702 GN=At3g01270 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3BH707.4e-21292.72Pectate lyase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003070... [more]
A0A1S3BF077.4e-21292.72Pectate lyase OS=Cucumis melo OX=3656 GN=LOC103488907 PE=3 SV=1[more]
A0A6J1C7639.0e-21081.59Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009024 PE=3 SV=1[more]
A0A0A0LR321.2e-20991.91Pectate lyase OS=Cucumis sativus OX=3659 GN=Csa_1G045510 PE=3 SV=1[more]
A0A6J1C7624.2e-20790.05Pectate lyase OS=Momordica charantia OX=3673 GN=LOC111009024 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018082AmbAllergenPRINTSPR00807AMBALLERGENcoord: 457..481
score: 51.24
coord: 313..334
score: 59.74
coord: 393..412
score: 76.19
coord: 213..238
score: 69.41
coord: 415..434
score: 80.48
coord: 189..206
score: 65.34
coord: 249..265
score: 62.18
IPR002022Pectate lyaseSMARTSM00656amb_allcoord: 241..438
e-value: 1.7E-99
score: 346.5
IPR002022Pectate lyasePFAMPF00544Pectate_lyase_4coord: 252..432
e-value: 2.9E-20
score: 72.8
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 166..507
e-value: 1.9E-114
score: 384.2
NoneNo IPR availablePANTHERPTHR31683:SF146PECTATE LYASEcoord: 141..514
IPR045032Pectin lyase familyPANTHERPTHR31683PECTATE LYASE 18-RELATEDcoord: 141..514
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 156..508

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Bhi08G001165Bhi08G001165gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi08M001165.exon4Bhi08M001165.exon4exon
Bhi08M001165.exon3Bhi08M001165.exon3exon
Bhi08M001165.exon2Bhi08M001165.exon2exon
Bhi08M001165.exon1Bhi08M001165.exon1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi08M001165.utr3p1Bhi08M001165.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Bhi08M001165cds.Bhi08M001165_4CDS
cds.Bhi08M001165cds.Bhi08M001165_3CDS
cds.Bhi08M001165cds.Bhi08M001165_2CDS
cds.Bhi08M001165cds.Bhi08M001165CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi08M001165.utr5p1Bhi08M001165.utr5p1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Bhi08M001165Bhi08M001165-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0030570 pectate lyase activity
molecular_function GO:0046872 metal ion binding