Bhi08M000361 (mRNA) Wax gourd (B227) v1

BLAST of Bhi08M000361 vs. TAIR 10
Match: AT2G30300.1 (Major facilitator superfamily protein )

HSP 1 Score: 417.2 bits (1071), Expect = 2.0e-116
Identity = 243/519 (46.82%), Postives = 336/519 (64.74%), Query Frame = 0

Query: 6   VFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGL 65
           +  WLSLV I+WLQS NGTN +FP+YSSQLK+ L +SQ +LN L+FASDAGK+    SG+
Sbjct: 5   ILPWLSLVAILWLQSFNGTNLSFPAYSSQLKELLKISQFKLNYLSFASDAGKVLGFISGI 64

Query: 66  AANYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWINTVCY 125
           AA YLPL LVL  G SLG  GYG+QYL +  +I                  ICWINT CY
Sbjct: 65  AAVYLPLPLVLLAGGSLGFAGYGLQYLSIIKKI------------------ICWINTACY 124

Query: 126 MVAITNFGTNRQIAVGISTSYQGLSAKIYTNIVSSIFSKHSSKTTETFLLLNSVLPMGVC 185
           +VAI +F  NRQ+AVGI+ SYQGLS KIYT++V S F     +    +LLLNS++P+  C
Sbjct: 125 IVAINSFPVNRQVAVGITASYQGLSGKIYTDMVHSFFHTSQREEASGYLLLNSLVPLVAC 184

Query: 186 VLASPLARIL---KVVEEQGKLEVGFFVIFVITIATGIFAAVTSVGSISRMLSPLSGLVG 245
           ++ +P+       K +     ++VGF V+FV+TIATGI+A  TS+ S+  +L     LVG
Sbjct: 185 LVTAPMLMRHGGDKTMSYSKDVKVGFIVLFVLTIATGIYAVATSLVSVPAVLV----LVG 244

Query: 246 IVVFLVLPLVVAVVEKVKESLKEGKERRVYHFTVEENKDVERVRGENQSKLERDGDSEEA 305
           I +FL+ PL + +    KE +   K ++  H   +    V++     +   + + + E+A
Sbjct: 245 IALFLLAPLAIPIGVGFKELMSSRKTQQKVH---DLEAPVDKFYFVEEDHTKEEEEFEKA 304

Query: 306 M----EEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSS 365
           +    EE+   ++ K+++FW+YFG+YLFG T+GL F NNLGQIAESRGS++ SSLV+LSS
Sbjct: 305 IIGVKEEVEWTQLWKKLDFWIYFGLYLFGPTVGLVFTNNLGQIAESRGSTATSSLVALSS 364

Query: 366 SFGFFGRLMPSILDYFLSRKKCMKSKPGWMVGLMGVLCGGFFLLLCPTDVSLCVSTAIIA 425
           SFGFFGRL+PS+LDYF SR K M S P  M G +  +   F LLL  +D++L + TA+I 
Sbjct: 365 SFGFFGRLLPSLLDYFFSRNKYMPSSPVSMAGSLVAMVASFLLLLIDSDIALYIGTAMIG 424

Query: 426 VCTGAITSISVSTTSELFGANNFSINHNLLVANIPFGSFLFGYMAAFLYHKQAG--GDGT 485
           + +GA+TS+SV+ T+ELFG  +F +NHN++V +IP GSF FG +AA +Y   A   GD  
Sbjct: 425 IFSGALTSLSVTMTAELFGTKHFGVNHNIVVGSIPLGSFSFGLLAAKIYRDGAALYGD-D 484

Query: 486 GKCIGVECYRTTFFIWGCFSSFGTLLALLLFARTKTFYS 516
           GKC G+ C++TT   WG   S   LLA +L+ R + FYS
Sbjct: 485 GKCFGMHCFQTTLVFWGMLCSIAALLAAVLYIRNRKFYS 497

BLAST of Bhi08M000361 vs. TAIR 10
Match: AT1G31470.1 (Major facilitator superfamily protein )

HSP 1 Score: 287.0 bits (733), Expect = 3.1e-77
Identity = 195/532 (36.65%), Postives = 295/532 (55.45%), Query Frame = 0

Query: 8   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 67
           +W  LV  IW+Q+  GTNF+F +YSS LK  L +SQ++LN LA ASD GK F   SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 68  NYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWINTVCYMV 127
            Y PL +VLF  A++G VGYGVQ+L +TN I  P Y ++FL  +LAG SICW NT C+++
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 128 AITNFGTNRQIAVGISTSYQGLSAKIYTNIVSSIFSKHSSKTTETFLLLNSVLPMGVC-- 187
            I +F  NR +A+ ++ S+ G+SA +Y    S  F+  +  ++  +LLLNS++P+ V   
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALY----SLAFNAINPSSSNLYLLLNSLVPLVVSFA 222

Query: 188 ----VLASPLARILKVVEEQGKLEVGFFVIFVITIATGIFAAVTSVGSISRMLSPLSGLV 247
               VL  P        + +      F ++ V+ + T     ++S  + S  L+     +
Sbjct: 223 ALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLN----FI 282

Query: 248 GIVVFLVLPLVVAVVEKVKESLKEGKERRVYH-------FTVEENKD----VERVRGENQ 307
           G VV LV PL   ++   ++        R+ H         ++E K+    V    G   
Sbjct: 283 GAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEH 342

Query: 308 SKLERDGDSEEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSV 367
               ++G++    +E   + ++ R+ FWLY+  Y  G T+GL + NNLGQIA+S G +S 
Sbjct: 343 MGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNS- 402

Query: 368 SSLVSLSSSFGFFGRLMPSILDYFLSRKKCMKSKPGWMVGLMGVLCGGFFLLLCPT--DV 427
           ++LV++ SSF FFGRL+ +  D+    K+   ++ GW    +      FFLL   +    
Sbjct: 403 TTLVTIYSSFSFFGRLLSAAPDFM--HKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQT 462

Query: 428 SLCVSTAIIAVCTGAITSISVSTTSELFGANNFSINHNLLVANIPFGSFLFGYMAAFLYH 487
           +L  +TA+I + +G I + +VS TS+LFG N+  +NHN+L+ NIP GS L+GY+AA +Y 
Sbjct: 463 ALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAASIYE 522

Query: 488 KQAGGDGTG------KCIGVECYRTTFFIWGCFSSFGTLLALLLFARTKTFY 515
             A  D T        CIG +CY  TF  WGC S  G + +L L+ RTK  Y
Sbjct: 523 ANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of Bhi08M000361 vs. TAIR 10
Match: AT5G45275.1 (Major facilitator superfamily protein )

HSP 1 Score: 279.3 bits (713), Expect = 6.5e-75
Identity = 206/561 (36.72%), Postives = 310/561 (55.26%), Query Frame = 0

Query: 8   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 67
           +W+ LV  IW+Q+  GTNF+F +YSS LK  L +SQ+QLN LA ASD GK+F   SGLA 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSTYSSNLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 68  NYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWINTVCYMV 127
            Y PLW VLF  A +G VGYGVQ+L +TN I  P Y ++FL  +LAG SICW NTVC+++
Sbjct: 67  LYFPLWTVLFAAAIMGFVGYGVQWLVITNVISLP-YILVFLCCLLAGLSICWFNTVCFVL 126

Query: 128 AITNFGTNRQIAVGISTSYQGLSAKIYTNIVSSIFSKHSSKTTETFLLLNSVLPMGVCVL 187
            I NF  NR +A+ ++ S+ G+SA +YT   ++I    +  +TE +LLLN+++P+ V   
Sbjct: 127 CIRNFPANRSLALSLTVSFNGVSAALYTLAYNAI----NPVSTELYLLLNALVPLFVSFA 186

Query: 188 A-SPLAR--ILKVVEEQG--KLEVGFFVIFVITIATGI----FAAVTSVGSISRMLSPLS 247
           A  P+ R   L+ +   G  +  + F ++ ++ +  G+    F + TS  + +R+L    
Sbjct: 187 ALIPILRQPPLEPLPPDGVRRDSLMFLLLNILAVLNGVYLLLFRSKTSDVTSARLL---- 246

Query: 248 GLVGIVVFLVLPLVV-------------------------AVVEKVKESLKEG---KERR 307
              G ++ L+LPL +                          +V+  +  + +G   +E  
Sbjct: 247 -FGGSILLLILPLCLPGLVYARNWYLHNIHSSFRLEGSGFILVDVDELEMHKGMVTREAS 306

Query: 308 VYHFTVEENKDVERVRGENQSKLERDGD----------SEEAM--EEIGAKEMVKRINFW 367
           +  + +  +  V  V   +Q     D D          ++  M  EE     ++ R +FW
Sbjct: 307 LEGYQLLNDDVVRAVNTPDQKSFIEDDDGCCCTKVITRNQLGMLGEEHPLSFLLCRSDFW 366

Query: 368 LYFGVYLFGATLGLAFLNNLGQIAESRG-SSSVSSLVSLSSSFGFFGRLMPSILDYFLSR 427
           LY+  Y  G T+GL + NNLGQIA+S G SS  ++LV+L SSF FFGRL+ +  DY   R
Sbjct: 367 LYYIAYFCGGTIGLVYSNNLGQIAQSLGQSSETTTLVTLYSSFSFFGRLLSATPDYI--R 426

Query: 428 KKCMKSKPGWM-VGLMGVLCGGFFLLLCPTDVSLCVSTAIIAVCTGAITSISVSTTSELF 487
            K   ++ GW+ V L+      F L    +  +L   TA+I + +G I + +VS TSELF
Sbjct: 427 AKVYFARTGWLAVALLPTTIALFLLASSGSLAALQAGTALIGLSSGFIFAAAVSITSELF 486

Query: 488 GANNFSINHNLLVANIPFGSFLFGYMAAFLY--HKQAGGDGTGK-CIGVECYRTTFFIWG 515
           G N+  +NHN+L+ NIP GS ++G++AA +Y  H  AG       C+G +CY  TF  WG
Sbjct: 487 GPNSVGVNHNILITNIPIGSLVYGFLAALVYESHSVAGSKTESVICMGRDCYLQTFMWWG 546

BLAST of Bhi08M000361 vs. TAIR 10
Match: AT4G19450.1 (Major facilitator superfamily protein )

HSP 1 Score: 272.3 bits (695), Expect = 8.0e-73
Identity = 201/561 (35.83%), Postives = 311/561 (55.44%), Query Frame = 0

Query: 8   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 67
           +W+ LV  IW+Q+  GTNF+F +YSS LK  L +SQ+QLN LA ASD GK+F   SGLA 
Sbjct: 7   KWMILVATIWIQAFTGTNFDFSAYSSDLKSVLGISQVQLNYLAVASDLGKVFGWSSGLAL 66

Query: 68  NYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWINTVCYMV 127
            Y PLW VLF  A +G VGYGVQ+L +T+ I  P Y ++FL  +LAG SICW NTVC+++
Sbjct: 67  MYFPLWTVLFAAAFMGFVGYGVQWLVITHFISLP-YIMVFLCCLLAGLSICWFNTVCFVL 126

Query: 128 AITNFGTNRQIAVGISTSYQGLSAKIYTNIVSSIFSKHSSKTTETFLLLNSVLPMGVCVL 187
            I+NF  NR +A+ ++ S+ G+SA +YT   ++I    +  + E +LLLN+++P+ V   
Sbjct: 127 CISNFPANRSLALSLTVSFNGVSAALYTLAYNAI----NPTSPELYLLLNALIPLIVSFT 186

Query: 188 A-SPLAR--ILKVVEEQG--KLEVGFFVIFVITIATGI----FAAVTSVGSISRMLSPLS 247
           A  P+ R    + +   G  +  + F ++ ++    G+    F + +S  + +R+L    
Sbjct: 187 AIIPILRQPPFEPLPPDGVRRDSLMFLLLNILAALNGVYLLLFGSNSSDLTSARLL--FG 246

Query: 248 GLVGIVVF-LVLPLVVAVVEKVKESLKEGKERRVYHFTVEENKDVERVRGENQSKLERDG 307
           G + ++VF L +P +V        ++          F + +  ++E  +G    +  R+G
Sbjct: 247 GAILLLVFPLCIPGLVIARNWYNRTIHTSFRLEGSGFILVDPDELELHKGMLAHEANREG 306

Query: 308 ---------------------DSEEA----------MEEIGAKE----MVKRINFWLYFG 367
                                DS+E+          +E +G +     ++ R +FWLY+ 
Sbjct: 307 YQLLSDDVVQNPVKSVAVEEEDSDESCCKKLITRDQLEGLGIEHSLSLLLTRSDFWLYYI 366

Query: 368 VYLFGATLGLAFLNNLGQIAESRG-SSSVSSLVSLSSSFGFFGRLMPSILDYFLSRKKCM 427
            Y  G T+GL + NNLGQIA+S G SS+ ++LV+L S+F FFGRL+ +  DY   R K  
Sbjct: 367 TYFCGGTIGLVYSNNLGQIAQSLGQSSNTTTLVTLYSAFSFFGRLLSATPDYI--RAKVY 426

Query: 428 KSKPGWM-VGLMGVLCGGFFLLLCPTDVSLCVSTAIIAVCTGAITSISVSTTSELFGANN 487
            ++ GW+ + L+      F L    T  +L   TA++ + +G I + +VS TSELFG N+
Sbjct: 427 FARTGWLAIALLPTPFALFLLASSGTASALQAGTALMGLSSGFIFAAAVSITSELFGPNS 486

Query: 488 FSINHNLLVANIPFGSFLFGYMAAFLYHKQAGGDGTGK-------CIGVECYRTTFFIWG 515
             +NHN+L+ NIP GS ++G++AA +Y    G  GT         C+G +CY  TF  WG
Sbjct: 487 VGVNHNILITNIPIGSLIYGFLAALVYDSH-GFTGTKSMTSESVVCMGRDCYYLTFVWWG 546

BLAST of Bhi08M000361 vs. TAIR 10
Match: AT3G01630.1 (Major facilitator superfamily protein )

HSP 1 Score: 266.9 bits (681), Expect = 3.4e-71
Identity = 188/529 (35.54%), Postives = 289/529 (54.63%), Query Frame = 0

Query: 8   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 67
           +W  LV  IW+Q+  GTNF+F +YSS +K  + +SQ +LN +A ASD GK     SG A 
Sbjct: 23  RWTVLVAAIWIQAFTGTNFDFSAYSSDMKSSMGVSQSRLNYMAVASDLGKALGWSSGFAI 82

Query: 68  NYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWINTVCYMV 127
            Y P+  VLF  A++GLVGYGVQ+L + + I  P Y ++ +   LAG SICW NTV +++
Sbjct: 83  AYFPVSGVLFAAAAMGLVGYGVQWLSIADVIDLP-YSLVLVCCSLAGLSICWFNTVSFIL 142

Query: 128 AITNFGTNRQIAVGISTSYQGLSAKIYTNIVSSIFSKHSSKTTETFLLLNSVLPMGVCVL 187
            I +F  N  +A+ +  S+ G+SA +YT +     S  SS +++ +LLLNS++P+ V VL
Sbjct: 143 CIRHFKANHSLALSLVVSFNGISAALYT-LGHEAISGKSSASSDIYLLLNSLIPLIVSVL 202

Query: 188 A------SPLARILKVVEEQGKLEVGFFVIFVITIATGIFAAVTSVGSISRMLSPLSGLV 247
           A      +P +          +  + F V  V+ + T  +  + S G+     SP    +
Sbjct: 203 ALWPVLTNPNSSETDTTRTHDETRI-FVVFNVLALVTCFYLLLPSSGTYLAS-SPRWHFL 262

Query: 248 GIVVFLVLPLVVAVVEKVKESLKEGKERRVYHF---TVEENKDVERVRGENQSKLERDGD 307
           G +  L+ PL V  ++ +  +L+         +    +EE K + +++ + +S  E + D
Sbjct: 263 GAIFLLLFPLCVPFLDYIHRALESCFHHHSSGYAAVNIEEPK-ILKIKSQ-KSNAEEESD 322

Query: 308 SEEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRG--SSSVSSLVSL 367
                +E     +V+++ FWLY+  Y  G T+GL + NNLGQIA+S G  SS+  SLV+L
Sbjct: 323 QVRLGDEHSLGMLVRKLEFWLYYVAYFCGGTIGLVYSNNLGQIAQSLGQSSSNAKSLVTL 382

Query: 368 SSSFGFFGRLMPSILDYFLSRKKCMKSKPGWM-VGLMGVLCGGFFLLLCP---TDVSLCV 427
            S+F F GRL+ S  D+   + K + ++ GW  + L+      F L   P       L V
Sbjct: 383 FSAFSFLGRLLSSAPDFTRKKLKYL-TRTGWFTISLLPTPLAFFILAYSPKTNQTALLEV 442

Query: 428 STAIIAVCTGAITSISVSTTSELFGANNFSINHNLLVANIPFGSFLFGYMAAFLY----- 487
           +TA+I + +G + + +VS TSELFG N+  +N N+L+ NIP GS  +GYMA  +Y     
Sbjct: 443 ATALIGLSSGFVFAAAVSITSELFGRNSVGVNQNILITNIPIGSLFYGYMAGSVYDTNAS 502

Query: 488 --HKQAGGDGTGKCIGVECYRTTFFIWGCFSSFGTLLALLLFARTKTFY 515
              K    D    C+G +CY  TF  WGC S  G + +L LF RT+  Y
Sbjct: 503 LGRKSVVADSV-VCVGRKCYFVTFLFWGCLSVLGFVCSLFLFIRTRPVY 543

BLAST of Bhi08M000361 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 287.0 bits (733), Expect = 4.4e-76
Identity = 195/532 (36.65%), Postives = 295/532 (55.45%), Query Frame = 0

Query: 8   QWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFSGLAA 67
           +W  LV  IW+Q+  GTNF+F +YSS LK  L +SQ++LN LA ASD GK F   SG+A 
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 68  NYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWINTVCYMV 127
            Y PL +VLF  A++G VGYGVQ+L +TN I  P Y ++FL  +LAG SICW NT C+++
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 128 AITNFGTNRQIAVGISTSYQGLSAKIYTNIVSSIFSKHSSKTTETFLLLNSVLPMGVC-- 187
            I +F  NR +A+ ++ S+ G+SA +Y    S  F+  +  ++  +LLLNS++P+ V   
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALY----SLAFNAINPSSSNLYLLLNSLVPLVVSFA 222

Query: 188 ----VLASPLARILKVVEEQGKLEVGFFVIFVITIATGIFAAVTSVGSISRMLSPLSGLV 247
               VL  P        + +      F ++ V+ + T     ++S  + S  L+     +
Sbjct: 223 ALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLN----FI 282

Query: 248 GIVVFLVLPLVVAVVEKVKESLKEGKERRVYH-------FTVEENKD----VERVRGENQ 307
           G VV LV PL   ++   ++        R+ H         ++E K+    V    G   
Sbjct: 283 GAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEH 342

Query: 308 SKLERDGDSEEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSV 367
               ++G++    +E   + ++ R+ FWLY+  Y  G T+GL + NNLGQIA+S G +S 
Sbjct: 343 MGTAKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLGQNS- 402

Query: 368 SSLVSLSSSFGFFGRLMPSILDYFLSRKKCMKSKPGWMVGLMGVLCGGFFLLLCPT--DV 427
           ++LV++ SSF FFGRL+ +  D+    K+   ++ GW    +      FFLL   +    
Sbjct: 403 TTLVTIYSSFSFFGRLLSAAPDFM--HKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQQT 462

Query: 428 SLCVSTAIIAVCTGAITSISVSTTSELFGANNFSINHNLLVANIPFGSFLFGYMAAFLYH 487
           +L  +TA+I + +G I + +VS TS+LFG N+  +NHN+L+ NIP GS L+GY+AA +Y 
Sbjct: 463 ALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGYIAASIYE 522

Query: 488 KQAGGDGTG------KCIGVECYRTTFFIWGCFSSFGTLLALLLFARTKTFY 515
             A  D T        CIG +CY  TF  WGC S  G + +L L+ RTK  Y
Sbjct: 523 ANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of Bhi08M000361 vs. ExPASy TrEMBL
Match: A0A1S3C569 (protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo OX=3656 GN=LOC103497028 PE=4 SV=1)

HSP 1 Score: 842.0 bits (2174), Expect = 1.3e-240
Identity = 454/528 (85.98%), Postives = 486/528 (92.05%), Query Frame = 0

Query: 2   SSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 61
           SSSIVFQWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC
Sbjct: 3   SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 62

Query: 62  FSGLAANYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWIN 121
           FSGLAANYLPLWLVLFIG+SLGL+GYGVQYLF+TNQ  SPSY +IF LTVLAGNSICWIN
Sbjct: 63  FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN 122

Query: 122 TVCYMVAITNF-GTNRQIAVGISTSYQGLSAKIYTNIVSSIFS-KHSSKTTETFLLLNSV 181
           TVCYMVAITNF  +NRQ+AVGISTSYQGLSAK+YT+IV SIFS K SSKT ETFLLLNSV
Sbjct: 123 TVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSV 182

Query: 182 LPMGVCVLASPLARILKVVEEQGKLEVGFFVIFVITIATGIFAAVTSVGSISRMLSPLSG 241
           LP+GVCVL SPLAR +K+VEEQGKLEVGFFVIFVITIATG FA +TSVGS+SRMLS LSG
Sbjct: 183 LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSG 242

Query: 242 LVGIVVFLVLPLVVAVVEKVKESLKEGKERRVYHFTVEENKDVERV-RGENQSKLERDGD 301
           LVGI+VFLVLPLVV V EKVKE ++EGKE RVYHFTVEEN DVER+ RGEN+ K+ER  D
Sbjct: 243 LVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERT-D 302

Query: 302 SEEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSS 361
             EAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSS
Sbjct: 303 EGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSS 362

Query: 362 SFGFFGRLMPSILDYFLSRK-KCMKSKPGWMVGLMGVLCGGFFLLLCPTDVSLCVSTAII 421
           SFGFFGRL+PSILDYFLSR+ K MKSKPGWMVGLMG LCGGFFLLL P+D SLC+STAII
Sbjct: 363 SFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCGGFFLLLSPSDTSLCMSTAII 422

Query: 422 AVCTGAITSISVSTTSELFGANNFSINHNLLVANIPFGSFLFGYMAAFLYHKQAG-GDGT 481
           A+CTGAITSISVSTT++LFGA NFSINHN++VANIPFGSF+FGYMAAFLYHKQAG G   
Sbjct: 423 AICTGAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVAA 482

Query: 482 GKCIGVECYRTTFFIWGCFSSFGTLLALLLFARTKTFYSTT--RSHTS 523
           GKCIGVECYRTTF IWGCFSSFGT LALLLFART++FYSTT   SH S
Sbjct: 483 GKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHIS 529

BLAST of Bhi08M000361 vs. ExPASy TrEMBL
Match: A0A0A0LYD4 (Nodulin-like domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G533690 PE=4 SV=1)

HSP 1 Score: 835.1 bits (2156), Expect = 1.6e-238
Identity = 447/521 (85.80%), Postives = 481/521 (92.32%), Query Frame = 0

Query: 2   SSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 61
           SSSIVFQWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC
Sbjct: 4   SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 63

Query: 62  FSGLAANYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWIN 121
           FSGLAANYLPLWLVLFIG+SLGL+GYGVQYLF+TNQ  SPSY +IF LTVLAGNSICWIN
Sbjct: 64  FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN 123

Query: 122 TVCYMVAITNF-GTNRQIAVGISTSYQGLSAKIYTNIVSSIFS-KHSSKTTETFLLLNSV 181
           TVCYMVAITNF  ++RQ+AVGISTSYQGLSAK+YT+IV SIFS KHSSKT ETFLLLNSV
Sbjct: 124 TVCYMVAITNFKSSSRQVAVGISTSYQGLSAKVYTDIVGSIFSNKHSSKTAETFLLLNSV 183

Query: 182 LPMGVCVLASPLARILKVVEEQGKLEVGFFVIFVITIATGIFAAVTSVGSISRMLSPLSG 241
           LP+GVCVL SPLAR +K+VEEQGKLEVGFFVIFVITIATGIFA +TSVGS+SRMLS L G
Sbjct: 184 LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGIFATMTSVGSVSRMLSALGG 243

Query: 242 LVGIVVFLVLPLVVAVVEKVKESLKEGKERRVYHFTVEENKDVERVRGENQSKLERDGDS 301
           LVGI+VFLVLPLVV VVEKVKE ++EGKE +VYHFTVEE  D ER+RGEN+ K+ER  D 
Sbjct: 244 LVGIMVFLVLPLVVVVVEKVKERVEEGKEGKVYHFTVEEKNDEERMRGENERKVERTDDG 303

Query: 302 EEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS 361
            EAMEEIGAKEMVKRINFWLY GVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS
Sbjct: 304 -EAMEEIGAKEMVKRINFWLYVGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSS 363

Query: 362 FGFFGRLMPSILDYFLSRK-KCMKSKPGWMVGLMGVLCGGFFLLLCPTDVSLCVSTAIIA 421
           FGFFGRL+PSILDYFLSR+ K MKSKPGWMVGLMG LCGGFFLLL P+D SLC+STAIIA
Sbjct: 364 FGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGTLCGGFFLLLSPSDTSLCMSTAIIA 423

Query: 422 VCTGAITSISVSTTSELFGANNFSINHNLLVANIPFGSFLFGYMAAFLYHKQAG-GDGTG 481
           +CTGAITSISVSTT++LFGA NFSINHN++VANIPFGSF+FGYMAAFLY KQAG G   G
Sbjct: 424 ICTGAITSISVSTTTDLFGATNFSINHNIVVANIPFGSFIFGYMAAFLYRKQAGHGVDPG 483

Query: 482 KCIGVECYRTTFFIWGCFSSFGTLLALLLFARTKTFYSTTR 519
           KCIGVECYRTTF IWG FSSFGT LALLLFARTK+FYSTT+
Sbjct: 484 KCIGVECYRTTFLIWGSFSSFGTFLALLLFARTKSFYSTTK 523

BLAST of Bhi08M000361 vs. ExPASy TrEMBL
Match: A0A6J1I3F0 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469568 PE=4 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 5.7e-228
Identity = 423/516 (81.98%), Postives = 465/516 (90.12%), Query Frame = 0

Query: 4   SIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFS 63
           S VFQWLSL+GIIWLQSINGTNFNFPSYSSQLK  LS+SQ QLNNLAFASDAGKLFAC S
Sbjct: 3   SSVFQWLSLLGIIWLQSINGTNFNFPSYSSQLKHHLSISQFQLNNLAFASDAGKLFACVS 62

Query: 64  GLAANYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWINTV 123
           GLAANYLPLW VL IG+SLGLVGYGVQYLF+TNQ  SPSYC+IF LTVLAGNSICWINTV
Sbjct: 63  GLAANYLPLWSVLIIGSSLGLVGYGVQYLFITNQFHSPSYCLIFFLTVLAGNSICWINTV 122

Query: 124 CYMVAITNFGTNRQIAVGISTSYQGLSAKIYTNIVSSIFSKHSSKTTETFLLLNSVLPMG 183
           CY+VAI NF +NRQ+AVGISTSYQGLSAKIYT+IV  IFSK S KT ETFLL NSV P+G
Sbjct: 123 CYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFPLG 182

Query: 184 VCVLASPLARILKVVEEQGKLEVGFFVIFVITIATGIFAAVTSVGSISRMLSPLSGLVGI 243
           VCVL SP+ARI+++VEEQG LEVGFFVIFVITIATG+FA VTSVGSISRMLSPLSGLVGI
Sbjct: 183 VCVLVSPMARIVELVEEQGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLVGI 242

Query: 244 VVFLVLPLVVAVVEKVKESLKEGKERRVYHFTVEENKDVERVRGENQSKLERDGDSEEAM 303
           +VFL+LP+VV VVE VK+S++EGKERRVYH T+EE+    R+R EN++KL RDG  +EAM
Sbjct: 243 LVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED---TRIRPENEAKLNRDG-GDEAM 302

Query: 304 EEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSFGFF 363
           EEIGA EM+KR+NFWLY GVY FGATLGLAFLNNLGQIAESRGS SVSSLVSLSSSFGFF
Sbjct: 303 EEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESRGSESVSSLVSLSSSFGFF 362

Query: 364 GRLMPSILDYFLSRKKCMKSKPGWMVGLMGVLCGGFFLLLCPTDVSLCVSTAIIAVCTGA 423
           GRLMPS+LD+FLSR+K MKSKPGW+V LMG +CGGFFLL  PTD+ LCVSTA+IAVCTGA
Sbjct: 363 GRLMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFLLSGPTDLILCVSTAVIAVCTGA 422

Query: 424 ITSISVSTTSELFGANNFSINHNLLVANIPFGSFLFGYMAAFLYHKQAGGDGTGKCIGVE 483
           ITSISVSTTSELFGANNFSINHN++VANIP GSFLFGYMAA+LY KQA G G GKCIGVE
Sbjct: 423 ITSISVSTTSELFGANNFSINHNVVVANIPVGSFLFGYMAAYLYRKQATG-GAGKCIGVE 482

Query: 484 CYRTTFFIWGCFSSFGTLLALLLFARTKTFYSTTRS 520
           CYRTTF IWGC  SFGTLLALLLFART++FYSTT+S
Sbjct: 483 CYRTTFMIWGCLCSFGTLLALLLFARTRSFYSTTKS 513

BLAST of Bhi08M000361 vs. ExPASy TrEMBL
Match: A0A5D3BBQ0 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00460 PE=4 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 9.1e-226
Identity = 435/528 (82.39%), Postives = 464/528 (87.88%), Query Frame = 0

Query: 2   SSSIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 61
           SSSIVFQWLSL+GIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC
Sbjct: 3   SSSIVFQWLSLIGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFAC 62

Query: 62  FSGLAANYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWIN 121
           FSGLAANYLPLWLVLFIG+SLGL+GYGVQYLF+TNQ  SPSY +IF LTVLAGNSICWIN
Sbjct: 63  FSGLAANYLPLWLVLFIGSSLGLIGYGVQYLFITNQFHSPSYWLIFFLTVLAGNSICWIN 122

Query: 122 TVCYMVAITNF-GTNRQIAVGISTSYQGLSAKIYTNIVSSIFS-KHSSKTTETFLLLNSV 181
           TVCYMVAITNF  +NRQ+AVGISTSYQGLSAK+YT+IV SIFS K SSKT ETFLLLNSV
Sbjct: 123 TVCYMVAITNFASSNRQVAVGISTSYQGLSAKVYTDIVGSIFSNKDSSKTAETFLLLNSV 182

Query: 182 LPMGVCVLASPLARILKVVEEQGKLEVGFFVIFVITIATGIFAAVTSVGSISRMLSPLSG 241
           LP+GVCVL SPLAR +K+VEEQGKLEVGFFVIFVITIATG FA +TSVGS+SRMLS LSG
Sbjct: 183 LPLGVCVLVSPLARFVKIVEEQGKLEVGFFVIFVITIATGTFATITSVGSVSRMLSALSG 242

Query: 242 LVGIVVFLVLPLVVAVVEKVKESLKEGKERRVYHFTVEENKDVERV-RGENQSKLERDGD 301
           LVGI+VFLVLPLVV V EKVKE ++EGKE RVYHFTVEEN DVER+ RGEN+ K+ER  D
Sbjct: 243 LVGIMVFLVLPLVVVVAEKVKERVEEGKEGRVYHFTVEENNDVERIMRGENERKVERT-D 302

Query: 302 SEEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSS 361
             EAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSS
Sbjct: 303 EGEAMEEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSS 362

Query: 362 SFGFFGRLMPSILDYFLSRK-KCMKSKPGWMVGLMGVLCGGFFLLLCPTDVSLCVSTAII 421
           SFGFFGRL+PSILDYFLSR+ K MKSKPGWMVGLMG LCG                    
Sbjct: 363 SFGFFGRLLPSILDYFLSRRNKFMKSKPGWMVGLMGALCG-------------------- 422

Query: 422 AVCTGAITSISVSTTSELFGANNFSINHNLLVANIPFGSFLFGYMAAFLYHKQAG-GDGT 481
               GAITSISVSTT++LFGA NFSINHN++VANIPFGSF+FGYMAAFLYHKQAG G   
Sbjct: 423 ----GAITSISVSTTTDLFGATNFSINHNVVVANIPFGSFIFGYMAAFLYHKQAGHGVAA 482

Query: 482 GKCIGVECYRTTFFIWGCFSSFGTLLALLLFARTKTFYSTT--RSHTS 523
           GKCIGVECYRTTF IWGCFSSFGT LALLLFART++FYSTT   SH S
Sbjct: 483 GKCIGVECYRTTFLIWGCFSSFGTFLALLLFARTRSFYSTTDLSSHIS 505

BLAST of Bhi08M000361 vs. ExPASy TrEMBL
Match: A0A6J1HMC6 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464885 PE=4 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 8.5e-224
Identity = 417/517 (80.66%), Postives = 462/517 (89.36%), Query Frame = 0

Query: 4   SIVFQWLSLVGIIWLQSINGTNFNFPSYSSQLKQQLSMSQLQLNNLAFASDAGKLFACFS 63
           S VFQWLSL+GIIWLQSINGTNFNFPSYSS LK  LS+SQ QLNNLAFASDAGKLFAC S
Sbjct: 57  SSVFQWLSLLGIIWLQSINGTNFNFPSYSSPLKHHLSISQFQLNNLAFASDAGKLFACVS 116

Query: 64  GLAANYLPLWLVLFIGASLGLVGYGVQYLFVTNQIQSPSYCVIFLLTVLAGNSICWINTV 123
           GLAANYLPLW +L IG+ LGLVGYGVQYLF+TNQ  SPSYC+IF L+VLAGNSICWINTV
Sbjct: 117 GLAANYLPLWSLLIIGSCLGLVGYGVQYLFITNQFHSPSYCLIFFLSVLAGNSICWINTV 176

Query: 124 CYMVAITNFGTNRQIAVGISTSYQGLSAKIYTNIVSSIFSKHSSKTTETFLLLNSVLPMG 183
           CY+VAI NF +NRQ+AVGISTSYQGLSAKIYT+IV  IFSK S KT ETFLL NSV P+G
Sbjct: 177 CYIVAIRNFPSNRQVAVGISTSYQGLSAKIYTDIVGLIFSKRSDKTAETFLLSNSVFPLG 236

Query: 184 VCVLASPLARILKVVEEQGKLEVGFFVIFVITIATGIFAAVTSVGSISRMLSPLSGLVGI 243
           VCVL  P+ARI+++VEE+G LEVGFFVIFVITIATG+FA VTSVGSISRMLSPLSGLVGI
Sbjct: 237 VCVLVCPMARIVELVEEKGNLEVGFFVIFVITIATGVFAVVTSVGSISRMLSPLSGLVGI 296

Query: 244 VVFLVLPLVVAVVEKVKESLKEGKERRVYHFTVEENKDVERVRGENQSKLERDGDSEEAM 303
           +VFL+LP+VV VVE VK+S++EGKERRVYH T+EE+    R+R EN++KL RDG  +EAM
Sbjct: 297 LVFLLLPVVVPVVESVKKSVREGKERRVYHSTMEED---TRIRPENEAKLNRDG-GDEAM 356

Query: 304 EEIGAKEMVKRINFWLYFGVYLFGATLGLAFLNNLGQIAESRGSSSVSSLVSLSSSFGFF 363
           EEIGA EM+KR+NFWLY GVY FGATLGLAFLNNLGQIAES GS SVSSLVSLSSSFGFF
Sbjct: 357 EEIGAMEMLKRVNFWLYVGVYFFGATLGLAFLNNLGQIAESWGSESVSSLVSLSSSFGFF 416

Query: 364 GRLMPSILDYFLSRKKCMKSKPGWMVGLMGVLCGGFFLLLC-PTDVSLCVSTAIIAVCTG 423
           GRLMPS+LD+FLSR+K MKSKPGW+V LMG +CGGFF LL  PTD+ LCVSTA+IAVCTG
Sbjct: 417 GRLMPSLLDHFLSRRKMMKSKPGWLVALMGAMCGGFFFLLSGPTDLILCVSTAVIAVCTG 476

Query: 424 AITSISVSTTSELFGANNFSINHNLLVANIPFGSFLFGYMAAFLYHKQAGGDGTGKCIGV 483
           AITSISVSTTSELFGANNFSINHN++VANIPFGSFLFGYMAA+LY KQA GD TGKCIGV
Sbjct: 477 AITSISVSTTSELFGANNFSINHNVVVANIPFGSFLFGYMAAYLYRKQATGD-TGKCIGV 536

Query: 484 ECYRTTFFIWGCFSSFGTLLALLLFARTKTFYSTTRS 520
           ECYRTTF IWGC  S GTLLALLLFART++FYSTT+S
Sbjct: 537 ECYRTTFMIWGCLCSLGTLLALLLFARTRSFYSTTKS 568