Bhi07M000624 (mRNA) Wax gourd (B227) v1

Overview
NameBhi07M000624
TypemRNA
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionNudix hydrolase
Locationchr7: 26345180 .. 26348409 (-)
Sequence length1205
RNA-Seq ExpressionBhi07M000624
SyntenyBhi07M000624
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGAATAAATAAATGAAGCGCGAAGCTTTGGATGAACGCCACTTGTGGAGGGAAAAGGAATGGCCGTAAACCGATATGTCGTTTTTAGTCATTCACTTCTTCACTATCGTCGTCCTCTTCGCCTTCGAAGTTCTCCTTCTCCACTTTTCTCATCTGTTCTGCGTTCGACCTCCTCATCCATGGAAGCTCCTCCCGAAGGTTACCGCAGGAATGTTGGCATTTGCCTCATCAATTCTTCTAAAAAGGTCATTCTCGAAACCTTTCTCTTTTTGTTCCTCCATCTCCGCTCATCTTTACAAGTATTCATCCATCTGCATCGTTCCGAAATCTGTATGTGCAGATTTTTGCTGCCTCAAGGCTGGATATACCAGATGCTTGGCAAATGCCTCAGGTAATTCGATGATTCTTTTTGCTTCTAGGTCATAGTTTTTCTTTTTCTTTTTTTTCTTTTTTAATCCGGAAGTTTGAGGTACAATCAGCATAACTTTGAGCTCTGAAATAACAATGAATTAGTACGTTAATTTACTGTTACATTGCTTGCTCTGGCCTCACTATTCAATTGTCGCGTATTAGGTTTTATGAAGAGTTTATTCGATCAGATGTATTCATTAAGTCTGAAATTTTAGCATTGAAGCATAATTAAATGGCGAGGAAATAATATATGATCATCTACGTATTTTCATAAGTGATTTATACGTTTTAGCCATTACGAGTTCATTTGTTCTGTTGTGTTCATTAGGGCGGCGTGGACGAGGATGAAGATCCAAGAAGTGCAGCCATCAGAGAACTTAGAGAAGAAACAGGAGTCAGATCCGCAGAGATTATTGCTGAAGTACTATTTCATTGATGTATTCGGTTGTCTTTCCTTGGATATGCATACTTCTCTTAAGTTTTCATAAATCAAGTGCTTCTCATAGTCGGTAAAACTGAAGAAAGTCTATTTTAGGAGTAAAGATGACTAGATTTTTTCTGTTCAAATGAAATTAGATCTCAACAATATCCAGTTTCTATTGCAGTTAATATGCTAAGGTACGAAAGAAAGTTCATAATTGATCACCACTGCATCGACCAAGCTCTTTGAATTAGTTTAGGGCATATTTGATGCTTTTCTTGTTCTCAACTTTACCATCAAATTTCTAACCATCAATTTTTTGATTAATGGAATTGGTTTATTGCAACTGAATCTTATTTTACCTTTTTTTCACTGACTAACTATTCGTGCTGGCTTTCATACACATCAACTAAGGTTAGCTCGTTAGACCATTATTTGGTCTTATAACATTTGATCTAAAAAAACAACTTGAAATATTATTGCTAGAGGGTCACCATAATCCAGATTTTAAAGAGAAATGAGCGTACATCATGCCTCTGATTACTGTGCCAATCTGTTGTAACCCATTGTGGTCATAATTTTCTTTAATCTCTGGAGATGGGGGAAGAATATTCTGAACTTTACCAGTGATCTCCCCTGCCCATTGACTCGGTTATTTAAAATTCTTCTACAAATATGGCTTGTTTCTTCTATGACCTTATGTAGTATGATTGCTGTGCGATAAGTTATGCTGTATCAGTAGTTTTAATTTGCATACTGTGTGGGTTACTGAAGTTTTGAATTCACCTTATATTTTTCTTCTTTCATTTTGGAGTTTTCCCAGAGTAAAAGCCTGTGTTTACCTTAATTGGACACATCAGTGAAATGTCTTGAATATTGGTTTCTTAAAGTAGGTACCTTATTGGCTGACTTATGACTTCCCACCACATGTCCGGGAAAAACTTAGACATCAGTGGGGTTCTGACTGGAAGGGTCAAGCGCAGAAGTGGTGAGTTCTATTTTCTATGCATACAGTTCTTATTCCTTTCATATTCTTTAGAGCTAAATCTCACTTGACGTACACTGGTGTACATAGTAATTAATACCTCAATGCTGATCCTTTTTATAAAAAAAGGCAAACAACCTTTGTGCCAAAACTCTGTTTCCTTATAGTAACTTCGAAAGCCGGTGCACCATACCGAAGTTGCTCACATTGGAGAGCAAAGCAACGAATTTTTGTGGGGGGTCTTAGTAATCATATAAAGGGCCAAGACAACTTAGACGTTCATTTCCTAACAATACATGTATTAAAGAAACAAAATCCACCCTCAGTGAATTTGTGGATAATAGTGTTAGTGTTACTTTTGAGTCTTCCTTCGTTTCTCTATTAGAATTCTGCAAGTAACTTTTTTCTCTTTCATTTTCTTTTCATCCTGCCAAGGCACTGTTTCATTTGCCTTATCAACTAAATTGTAACGATGTTTGCAAAATTTTAGGTTTTTGCTGAAGTTGACAGGAAGTGATGAAGAGATCAATCTTTTGGGTGATGGGACTGAGAAAGCGGAGTTTGGCGAATGGTCATGGATGTCACCTGAAGAAGTAGTAGAACGTGTGAGTACACCACTGTTACCTTTTTATTTAATTCAACAATTGTAAGGTGGGAGATCGAACTATCAATCTTTGAAACAGTAATTGATACATTATCAATTGAACTATGCTCGAAATGACAGACGTTCGTTAACTCATCATCAACAAAAAAACAGCTGAAAAACGAATATGAATATTCAACTTACATTTACCCTCTATCATGCAGGCTGTAGATTTCAAGAAACCTGTTTACAAGGACGTGCTAACGGTATTCAAACCCCATCTCGAGTAATATTATCCTATACTAAAAACATTTATTCTTGCCCCCTTTCAATAATGTTGGTTTTATTTTTGTCCAATGAACTATGTTAATATTATATAGAAGGAGGCTTTGTCCAATCTCCAAGCTGTGACGACATATCAAACCAGCTTGATTGGGGAAGTAATCTTCTACTCTTCTTACATGATGGTTAGTTATAGGATCGTTTTCCCCCTCTCTTTTTGGAACGAGAATATTGGAAACGAGTTCATGTTTCATTTTATAGTAGAATGCTCTCGTATCTTTGAGAATAAATGATACTTTATTAAATGGGAACAAGACTCCATCTAGGTTTGCAGACGGGACATTTGGCTGCTATTGATATTATACCAATTGATAAAGACAACGGACCTTCACATCCAATCAGTGGTCTACTAGTATTCAAATTATAGTTGCATTTTTATTTAAAAAGGTTGTCCTCTCGATATAGGGCCTTACACATTGTGAAGTTTTAAAATCGACTGACATTATTAGTTTTACTAATTTAATATTTAATTTAGTTAATTGTAAA

mRNA sequence

AAAAGAATAAATAAATGAAGCGCGAAGCTTTGGATGAACGCCACTTGTGGAGGGAAAAGGAATGGCCGTAAACCGATATGTCGTTTTTAGTCATTCACTTCTTCACTATCGTCGTCCTCTTCGCCTTCGAAGTTCTCCTTCTCCACTTTTCTCATCTGTTCTGCGTTCGACCTCCTCATCCATGGAAGCTCCTCCCGAAGGTTACCGCAGGAATGTTGGCATTTGCCTCATCAATTCTTCTAAAAAGATTTTTGCTGCCTCAAGGCTGGATATACCAGATGCTTGGCAAATGCCTCAGGGCGGCGTGGACGAGGATGAAGATCCAAGAAGTGCAGCCATCAGAGAACTTAGAGAAGAAACAGGAGTCAGATCCGCAGAGATTATTGCTGAAGTACCTTATTGGCTGACTTATGACTTCCCACCACATGTCCGGGAAAAACTTAGACATCAGTGGGGTTCTGACTGGAAGGGTCAAGCGCAGAAGTGGTTTTTGCTGAAGTTGACAGGAAGTGATGAAGAGATCAATCTTTTGGGTGATGGGACTGAGAAAGCGGAGTTTGGCGAATGGTCATGGATGTCACCTGAAGAAGTAGTAGAACGTGCTGTAGATTTCAAGAAACCTGTTTACAAGGACGTGCTAACGGTATTCAAACCCCATCTCGAGTAATATTATCCTATACTAAAAACATTTATTCTTGCCCCCTTTCAATAATGTTGGTTTTATTTTTGTCCAATGAACTATGTTAATATTATATAGAAGGAGGCTTTGTCCAATCTCCAAGCTGTGACGACATATCAAACCAGCTTGATTGGGGAAGTAATCTTCTACTCTTCTTACATGATGGTTAGTTATAGGATCGTTTTCCCCCTCTCTTTTTGGAACGAGAATATTGGAAACGAGTTCATGTTTCATTTTATAGTAGAATGCTCTCGTATCTTTGAGAATAAATGATACTTTATTAAATGGGAACAAGACTCCATCTAGGTTTGCAGACGGGACATTTGGCTGCTATTGATATTATACCAATTGATAAAGACAACGGACCTTCACATCCAATCAGTGGTCTACTAGTATTCAAATTATAGTTGCATTTTTATTTAAAAAGGTTGTCCTCTCGATATAGGGCCTTACACATTGTGAAGTTTTAAAATCGACTGACATTATTAGTTTTACTAATTTAATATTTAATTTAGTTAATTGTAAA

Coding sequence (CDS)

ATGGCCGTAAACCGATATGTCGTTTTTAGTCATTCACTTCTTCACTATCGTCGTCCTCTTCGCCTTCGAAGTTCTCCTTCTCCACTTTTCTCATCTGTTCTGCGTTCGACCTCCTCATCCATGGAAGCTCCTCCCGAAGGTTACCGCAGGAATGTTGGCATTTGCCTCATCAATTCTTCTAAAAAGATTTTTGCTGCCTCAAGGCTGGATATACCAGATGCTTGGCAAATGCCTCAGGGCGGCGTGGACGAGGATGAAGATCCAAGAAGTGCAGCCATCAGAGAACTTAGAGAAGAAACAGGAGTCAGATCCGCAGAGATTATTGCTGAAGTACCTTATTGGCTGACTTATGACTTCCCACCACATGTCCGGGAAAAACTTAGACATCAGTGGGGTTCTGACTGGAAGGGTCAAGCGCAGAAGTGGTTTTTGCTGAAGTTGACAGGAAGTGATGAAGAGATCAATCTTTTGGGTGATGGGACTGAGAAAGCGGAGTTTGGCGAATGGTCATGGATGTCACCTGAAGAAGTAGTAGAACGTGCTGTAGATTTCAAGAAACCTGTTTACAAGGACGTGCTAACGGTATTCAAACCCCATCTCGAGTAA

Protein sequence

MAVNRYVVFSHSLLHYRRPLRLRSSPSPLFSSVLRSTSSSMEAPPEGYRRNVGICLINSSKKIFAASRLDIPDAWQMPQGGVDEDEDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFPPHVREKLRHQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEEVVERAVDFKKPVYKDVLTVFKPHLE
Homology
BLAST of Bhi07M000624 vs. TAIR 10
Match: AT3G10620.1 (nudix hydrolase homolog 26 )

HSP 1 Score: 285.8 bits (730), Expect = 2.7e-77
Identity = 134/183 (73.22%), Postives = 153/183 (83.61%), Query Frame = 0

Query: 19  PLRLRSSPSPLFSSVLRSTSSSMEAPPEGYRRNVGICLINSSKKIFAASRLDIPDAWQMP 78
           P   R   S + SS  R   SSME+PPEGYRRNVG+CL+NSSKKIF ASRLDIP AWQMP
Sbjct: 35  PFLHRCRKSRVSSSSAR-CCSSMESPPEGYRRNVGVCLMNSSKKIFTASRLDIPSAWQMP 94

Query: 79  QGGVDEDEDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFPPHVREKLRHQWGSDWKGQ 138
           QGG+DE EDPR A +REL+EETGV SAEI+AE P+W+TYDFPP VREKL+ +WGSDWKGQ
Sbjct: 95  QGGIDEGEDPRVAVMRELKEETGVHSAEILAEAPHWITYDFPPDVREKLKVRWGSDWKGQ 154

Query: 139 AQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEEVVERAVDFKKPVYKDVLTVFKP 198
           AQKWFLLK TG DEEINLLGDGTEK EFGEWSW SP++VVE AV+FKKPVYK+V++ F  
Sbjct: 155 AQKWFLLKFTGKDEEINLLGDGTEKPEFGEWSWTSPDQVVENAVEFKKPVYKEVMSAFAS 214

Query: 199 HLE 202
           HL+
Sbjct: 215 HLQ 216

BLAST of Bhi07M000624 vs. TAIR 10
Match: AT5G06340.1 (nudix hydrolase homolog 27 )

HSP 1 Score: 239.6 bits (610), Expect = 2.2e-63
Identity = 112/178 (62.92%), Postives = 136/178 (76.40%), Query Frame = 0

Query: 24  SSPSPL--FSSVLRSTSSSMEAPPEGYRRNVGICLINSSKKIFAASRLDIPDAWQMPQGG 83
           SSP PL   S  L S + ++E+PP GYR+NVGICL++  +KIF AS++ IPD WQMPQGG
Sbjct: 36  SSPKPLVVLSVALSSPARTVESPPVGYRKNVGICLVSPCRKIFTASKIHIPDTWQMPQGG 95

Query: 84  VDEDEDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFPPHVREKLRHQWGSDWKGQAQK 143
            DE ED R+AA RELREETGV SAE IAE+P WLTYDFP  V++KL  +W + +KGQAQK
Sbjct: 96  ADEGEDLRNAAFRELREETGVTSAEFIAEIPNWLTYDFPREVKDKLNRKWRTSYKGQAQK 155

Query: 144 WFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEEVVERAVDFKKPVYKDVLTVFKPH 200
           WFL K TG +EEINLLGDGT K EF  WSWM PE+V+E AV FK+PVY+ V+  F P+
Sbjct: 156 WFLFKFTGKEEEINLLGDGTAKPEFKVWSWMLPEQVIEHAVYFKRPVYEHVIKQFNPY 213

BLAST of Bhi07M000624 vs. TAIR 10
Match: AT1G30110.1 (nudix hydrolase homolog 25 )

HSP 1 Score: 208.8 bits (530), Expect = 4.2e-54
Identity = 91/157 (57.96%), Postives = 120/157 (76.43%), Query Frame = 0

Query: 41  MEAPPEGYRRNVGICLINSSKKIFAASRLDIPDAWQMPQGGVDEDEDPRSAAIRELREET 100
           ME  P GYR NVG+CLINS   +F ASRL++P AWQMPQGG+++ EDP+SAA+REL+EET
Sbjct: 1   MENLPPGYRPNVGVCLINSDNLVFVASRLNVPGAWQMPQGGIEDGEDPKSAAMRELQEET 60

Query: 101 GVRSAEIIAEVPYWLTYDFPPHVREKLRHQWGSDWKGQAQKWFLLKLTGSDEEINL-LGD 160
           GV SAEI++EVP WLTYDFPP V+ K+   WG +W GQAQKW+L++L   ++E  + L +
Sbjct: 61  GVVSAEIVSEVPNWLTYDFPPAVKAKVNRLWGGEWHGQAQKWYLVRLRNDEDEKEINLAN 120

Query: 161 GTEKAEFGEWSWMSPEEVVERAVDFKKPVYKDVLTVF 197
               +EF EW W  PEEVVE+AVD+K+P Y++V+  F
Sbjct: 121 NEADSEFAEWKWAKPEEVVEQAVDYKRPTYEEVIKTF 157

BLAST of Bhi07M000624 vs. TAIR 10
Match: AT1G30110.2 (nudix hydrolase homolog 25 )

HSP 1 Score: 208.8 bits (530), Expect = 4.2e-54
Identity = 91/157 (57.96%), Postives = 120/157 (76.43%), Query Frame = 0

Query: 41  MEAPPEGYRRNVGICLINSSKKIFAASRLDIPDAWQMPQGGVDEDEDPRSAAIRELREET 100
           ME  P GYR NVG+CLINS   +F ASRL++P AWQMPQGG+++ EDP+SAA+REL+EET
Sbjct: 1   MENLPPGYRPNVGVCLINSDNLVFVASRLNVPGAWQMPQGGIEDGEDPKSAAMRELQEET 60

Query: 101 GVRSAEIIAEVPYWLTYDFPPHVREKLRHQWGSDWKGQAQKWFLLKLTGSDEEINL-LGD 160
           GV SAEI++EVP WLTYDFPP V+ K+   WG +W GQAQKW+L++L   ++E  + L +
Sbjct: 61  GVVSAEIVSEVPNWLTYDFPPAVKAKVNRLWGGEWHGQAQKWYLVRLRNDEDEKEINLAN 120

Query: 161 GTEKAEFGEWSWMSPEEVVERAVDFKKPVYKDVLTVF 197
               +EF EW W  PEEVVE+AVD+K+P Y++V+  F
Sbjct: 121 NEADSEFAEWKWAKPEEVVEQAVDYKRPTYEEVIKTF 157

BLAST of Bhi07M000624 vs. TAIR 10
Match: AT4G25434.2 (nudix hydrolase homolog 10 )

HSP 1 Score: 42.7 bits (99), Expect = 4.0e-04
Identity = 25/56 (44.64%), Postives = 36/56 (64.29%), Query Frame = 0

Query: 48  YRRNVGICLINSSKKIFAASRLDIPDAWQMPQGGVDEDEDPRSAAIRELREETGVR 104
           +R  VG  ++N +K+    S L     W++P G VDE E+  +AAIRE++EETGVR
Sbjct: 111 HRVRVGAVVLNHNKEEKYGS-LCGSGIWKIPTGVVDEGEEIFAAAIREVKEETGVR 165

BLAST of Bhi07M000624 vs. ExPASy Swiss-Prot
Match: Q9CAF2 (Nudix hydrolase 26, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT26 PE=1 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 3.8e-76
Identity = 134/183 (73.22%), Postives = 153/183 (83.61%), Query Frame = 0

Query: 19  PLRLRSSPSPLFSSVLRSTSSSMEAPPEGYRRNVGICLINSSKKIFAASRLDIPDAWQMP 78
           P   R   S + SS  R   SSME+PPEGYRRNVG+CL+NSSKKIF ASRLDIP AWQMP
Sbjct: 35  PFLHRCRKSRVSSSSAR-CCSSMESPPEGYRRNVGVCLMNSSKKIFTASRLDIPSAWQMP 94

Query: 79  QGGVDEDEDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFPPHVREKLRHQWGSDWKGQ 138
           QGG+DE EDPR A +REL+EETGV SAEI+AE P+W+TYDFPP VREKL+ +WGSDWKGQ
Sbjct: 95  QGGIDEGEDPRVAVMRELKEETGVHSAEILAEAPHWITYDFPPDVREKLKVRWGSDWKGQ 154

Query: 139 AQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEEVVERAVDFKKPVYKDVLTVFKP 198
           AQKWFLLK TG DEEINLLGDGTEK EFGEWSW SP++VVE AV+FKKPVYK+V++ F  
Sbjct: 155 AQKWFLLKFTGKDEEINLLGDGTEKPEFGEWSWTSPDQVVENAVEFKKPVYKEVMSAFAS 214

Query: 199 HLE 202
           HL+
Sbjct: 215 HLQ 216

BLAST of Bhi07M000624 vs. ExPASy Swiss-Prot
Match: Q9FNH4 (Nudix hydrolase 27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT27 PE=1 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 3.1e-62
Identity = 112/178 (62.92%), Postives = 136/178 (76.40%), Query Frame = 0

Query: 24  SSPSPL--FSSVLRSTSSSMEAPPEGYRRNVGICLINSSKKIFAASRLDIPDAWQMPQGG 83
           SSP PL   S  L S + ++E+PP GYR+NVGICL++  +KIF AS++ IPD WQMPQGG
Sbjct: 36  SSPKPLVVLSVALSSPARTVESPPVGYRKNVGICLVSPCRKIFTASKIHIPDTWQMPQGG 95

Query: 84  VDEDEDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFPPHVREKLRHQWGSDWKGQAQK 143
            DE ED R+AA RELREETGV SAE IAE+P WLTYDFP  V++KL  +W + +KGQAQK
Sbjct: 96  ADEGEDLRNAAFRELREETGVTSAEFIAEIPNWLTYDFPREVKDKLNRKWRTSYKGQAQK 155

Query: 144 WFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEEVVERAVDFKKPVYKDVLTVFKPH 200
           WFL K TG +EEINLLGDGT K EF  WSWM PE+V+E AV FK+PVY+ V+  F P+
Sbjct: 156 WFLFKFTGKEEEINLLGDGTAKPEFKVWSWMLPEQVIEHAVYFKRPVYEHVIKQFNPY 213

BLAST of Bhi07M000624 vs. ExPASy Swiss-Prot
Match: Q9C6Z2 (Nudix hydrolase 25 OS=Arabidopsis thaliana OX=3702 GN=NUDT25 PE=1 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 5.9e-53
Identity = 91/157 (57.96%), Postives = 120/157 (76.43%), Query Frame = 0

Query: 41  MEAPPEGYRRNVGICLINSSKKIFAASRLDIPDAWQMPQGGVDEDEDPRSAAIRELREET 100
           ME  P GYR NVG+CLINS   +F ASRL++P AWQMPQGG+++ EDP+SAA+REL+EET
Sbjct: 1   MENLPPGYRPNVGVCLINSDNLVFVASRLNVPGAWQMPQGGIEDGEDPKSAAMRELQEET 60

Query: 101 GVRSAEIIAEVPYWLTYDFPPHVREKLRHQWGSDWKGQAQKWFLLKLTGSDEEINL-LGD 160
           GV SAEI++EVP WLTYDFPP V+ K+   WG +W GQAQKW+L++L   ++E  + L +
Sbjct: 61  GVVSAEIVSEVPNWLTYDFPPAVKAKVNRLWGGEWHGQAQKWYLVRLRNDEDEKEINLAN 120

Query: 161 GTEKAEFGEWSWMSPEEVVERAVDFKKPVYKDVLTVF 197
               +EF EW W  PEEVVE+AVD+K+P Y++V+  F
Sbjct: 121 NEADSEFAEWKWAKPEEVVEQAVDYKRPTYEEVIKTF 157

BLAST of Bhi07M000624 vs. ExPASy Swiss-Prot
Match: Q3IZC1 (RNA pyrophosphohydrolase OS=Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158 / JCM 6121 / NBRC 12203 / NCIMB 8253 / ATH 2.4.1.) OX=272943 GN=rppH PE=3 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 3.1e-38
Identity = 81/156 (51.92%), Postives = 101/156 (64.74%), Query Frame = 0

Query: 48  YRRNVGICLINSSKKIFAASRLDIP-DAWQMPQGGVDEDEDPRSAAIRELREETGV--RS 107
           YR  VGI LIN    IFA  R+D P  AWQMPQGG+DE E PR AA+REL EETG+    
Sbjct: 12  YRPCVGIVLINREGLIFAGQRIDSPVPAWQMPQGGIDEGEKPREAALRELWEETGIPAER 71

Query: 108 AEIIAEVPYWLTYDFPPHVREKLRHQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKA 167
            E +A+ P W+TYD PP   E L   WG  ++GQ QKWFL +  G+DEE+   G GT+ A
Sbjct: 72  VEFVAKAPDWVTYDLPP---ELLGRVWGGKYRGQRQKWFLYRYLGTDEEV---GIGTDHA 131

Query: 168 EFGEWSWMSPEEVVERAVDFKKPVYKDVLTVFKPHL 201
           EF  W W+  EE+V   V FK+ VY++V+  F+PHL
Sbjct: 132 EFSCWRWIGAEEMVAAIVPFKRAVYEEVVATFRPHL 161

BLAST of Bhi07M000624 vs. ExPASy Swiss-Prot
Match: B9KN59 (RNA pyrophosphohydrolase OS=Rhodobacter sphaeroides (strain KD131 / KCTC 12085) OX=557760 GN=rppH PE=3 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 3.1e-38
Identity = 81/156 (51.92%), Postives = 101/156 (64.74%), Query Frame = 0

Query: 48  YRRNVGICLINSSKKIFAASRLDIP-DAWQMPQGGVDEDEDPRSAAIRELREETGV--RS 107
           YR  VGI LIN    IFA  R+D P  AWQMPQGG+DE E PR AA+REL EETG+    
Sbjct: 12  YRPCVGIVLINREGLIFAGQRIDSPVPAWQMPQGGIDEGEKPREAALRELWEETGIPAER 71

Query: 108 AEIIAEVPYWLTYDFPPHVREKLRHQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKA 167
            E +A+ P W+TYD PP   E L   WG  ++GQ QKWFL +  G+DEE+   G GT+ A
Sbjct: 72  VEFVAKAPDWVTYDLPP---ELLGRVWGGKYRGQRQKWFLYRYLGTDEEV---GIGTDHA 131

Query: 168 EFGEWSWMSPEEVVERAVDFKKPVYKDVLTVFKPHL 201
           EF  W W+  EE+V   V FK+ VY++V+  F+PHL
Sbjct: 132 EFSCWRWIGAEEMVAAIVPFKRAVYEEVVATFRPHL 161

BLAST of Bhi07M000624 vs. ExPASy TrEMBL
Match: A0A6J1HWY8 (nudix hydrolase 26, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111467610 PE=3 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 4.6e-101
Identity = 182/201 (90.55%), Postives = 188/201 (93.53%), Query Frame = 0

Query: 1   MAVNRYVVFSHSLLHYRRPLRLRSSPSPLFSSVLRSTSSSMEAPPEGYRRNVGICLINSS 60
           MA++R VVF+HSLLHY R       P PLFS+ LRSTSSSMEAPPEGYRRNVGICL+NSS
Sbjct: 1   MAISRCVVFNHSLLHYHR------RPPPLFSTALRSTSSSMEAPPEGYRRNVGICLVNSS 60

Query: 61  KKIFAASRLDIPDAWQMPQGGVDEDEDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFP 120
           KKIFAASRLDIPDAWQMPQGGVDE EDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFP
Sbjct: 61  KKIFAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFP 120

Query: 121 PHVREKLRHQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEEVVER 180
           PHVREKLRHQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEK EFGEWSWMSPE+VVER
Sbjct: 121 PHVREKLRHQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKPEFGEWSWMSPEQVVER 180

Query: 181 AVDFKKPVYKDVLTVFKPHLE 202
           AVDFKKPVYKDVLTVFKPHLE
Sbjct: 181 AVDFKKPVYKDVLTVFKPHLE 195

BLAST of Bhi07M000624 vs. ExPASy TrEMBL
Match: A0A6J1FZW7 (nudix hydrolase 26, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111449436 PE=3 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.3e-100
Identity = 183/201 (91.04%), Postives = 187/201 (93.03%), Query Frame = 0

Query: 1   MAVNRYVVFSHSLLHYRRPLRLRSSPSPLFSSVLRSTSSSMEAPPEGYRRNVGICLINSS 60
           MA++R VVF+HSLLHY R       P  LFSS LRSTSSSMEAPPEGYRRNVGICLINSS
Sbjct: 17  MAISRCVVFNHSLLHYHR------RPPSLFSSALRSTSSSMEAPPEGYRRNVGICLINSS 76

Query: 61  KKIFAASRLDIPDAWQMPQGGVDEDEDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFP 120
           KKIFAASRLDIPDAWQMPQGGVDE EDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFP
Sbjct: 77  KKIFAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFP 136

Query: 121 PHVREKLRHQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEEVVER 180
           PHVREKLRHQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEK EFGEWSWMSPE+VVER
Sbjct: 137 PHVREKLRHQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKPEFGEWSWMSPEQVVER 196

Query: 181 AVDFKKPVYKDVLTVFKPHLE 202
           AVDFKKPVYKDVLTVFKPHLE
Sbjct: 197 AVDFKKPVYKDVLTVFKPHLE 211

BLAST of Bhi07M000624 vs. ExPASy TrEMBL
Match: A0A1S3BGM3 (nudix hydrolase 26, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489378 PE=3 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 2.5e-99
Identity = 180/201 (89.55%), Postives = 185/201 (92.04%), Query Frame = 0

Query: 1   MAVNRYVVFSHSLLHYRRPLRLRSSPSPLFSSVLRSTSSSMEAPPEGYRRNVGICLINSS 60
           MA+NRYVVFSHSL HYRR       P P FSS LRSTSSSMEAPP GYR+NVGICLIN S
Sbjct: 1   MALNRYVVFSHSLFHYRR-------PPPFFSSALRSTSSSMEAPPAGYRKNVGICLINPS 60

Query: 61  KKIFAASRLDIPDAWQMPQGGVDEDEDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFP 120
           KKIFAASRLDIPDAWQMPQGGVDE EDPRSAAIRELREETGV+SA+IIAEVPYW+TYDFP
Sbjct: 61  KKIFAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVKSADIIAEVPYWVTYDFP 120

Query: 121 PHVREKLRHQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEEVVER 180
           P VREKLR QWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEEVVER
Sbjct: 121 PQVREKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEEVVER 180

Query: 181 AVDFKKPVYKDVLTVFKPHLE 202
           AVDFKKPVYKDVLTVFKPHLE
Sbjct: 181 AVDFKKPVYKDVLTVFKPHLE 194

BLAST of Bhi07M000624 vs. ExPASy TrEMBL
Match: A0A0A0KRB9 (Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G613530 PE=3 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.6e-98
Identity = 178/201 (88.56%), Postives = 184/201 (91.54%), Query Frame = 0

Query: 1   MAVNRYVVFSHSLLHYRRPLRLRSSPSPLFSSVLRSTSSSMEAPPEGYRRNVGICLINSS 60
           MA+NR VV SHSL HYRRP      P P FSS LRSTSSSMEAPPEGYR+NVGICLIN S
Sbjct: 1   MALNRCVVLSHSLFHYRRP-----PPPPFFSSALRSTSSSMEAPPEGYRKNVGICLINPS 60

Query: 61  KKIFAASRLDIPDAWQMPQGGVDEDEDPRSAAIRELREETGVRSAEIIAEVPYWLTYDFP 120
           KKIFAASRLDIPDAWQMPQGGVDE EDPRSAAIRELREETGV+SA+IIAEVPYW+TYDFP
Sbjct: 61  KKIFAASRLDIPDAWQMPQGGVDEGEDPRSAAIRELREETGVKSADIIAEVPYWVTYDFP 120

Query: 121 PHVREKLRHQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPEEVVER 180
           PHVREKLR QWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEK EFGEWSWMSPE+VVE 
Sbjct: 121 PHVREKLRQQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKPEFGEWSWMSPEDVVES 180

Query: 181 AVDFKKPVYKDVLTVFKPHLE 202
           AVDFKKPVYKDVLTVFKPHLE
Sbjct: 181 AVDFKKPVYKDVLTVFKPHLE 196

BLAST of Bhi07M000624 vs. ExPASy TrEMBL
Match: A0A6J1DAT2 (nudix hydrolase 26, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018605 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 2.8e-98
Identity = 181/206 (87.86%), Postives = 192/206 (93.20%), Query Frame = 0

Query: 1   MAVNRYVVFSHS-LLHYRRPLRLR----SSPSPLFSSVLRSTSSSMEAPPEGYRRNVGIC 60
           MA+NR VVF+HS LLH RRPLRLR    +SP+ LFSS  R  SSSMEAPPEGYRRNVGIC
Sbjct: 10  MALNRPVVFTHSVLLHCRRPLRLRLRLPTSPAALFSSAARPASSSMEAPPEGYRRNVGIC 69

Query: 61  LINSSKKIFAASRLDIPDAWQMPQGGVDEDEDPRSAAIRELREETGVRSAEIIAEVPYWL 120
           LINSSKKIFAASRLDIP+AWQMPQGGVDE EDPR+AAIRELREETGVRSAEIIAEVPYWL
Sbjct: 70  LINSSKKIFAASRLDIPEAWQMPQGGVDEGEDPRNAAIRELREETGVRSAEIIAEVPYWL 129

Query: 121 TYDFPPHVREKLRHQWGSDWKGQAQKWFLLKLTGSDEEINLLGDGTEKAEFGEWSWMSPE 180
           TYDFPPHVREKLRHQWGSDWKGQAQKWFLLKLTG++EEINLLGDGTEKAEFGEWSWMSPE
Sbjct: 130 TYDFPPHVREKLRHQWGSDWKGQAQKWFLLKLTGTEEEINLLGDGTEKAEFGEWSWMSPE 189

Query: 181 EVVERAVDFKKPVYKDVLTVFKPHLE 202
           +V+ERAVDFKKPVYKDVL VFKP+LE
Sbjct: 190 QVLERAVDFKKPVYKDVLMVFKPYLE 215

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT3G10620.12.7e-7773.22nudix hydrolase homolog 26 [more]
AT5G06340.12.2e-6362.92nudix hydrolase homolog 27 [more]
AT1G30110.14.2e-5457.96nudix hydrolase homolog 25 [more]
AT1G30110.24.2e-5457.96nudix hydrolase homolog 25 [more]
AT4G25434.24.0e-0444.64nudix hydrolase homolog 10 [more]
Match NameE-valueIdentityDescription
Q9CAF23.8e-7673.22Nudix hydrolase 26, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT26 PE=1... [more]
Q9FNH43.1e-6262.92Nudix hydrolase 27, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NUDT27 PE=1... [more]
Q9C6Z25.9e-5357.96Nudix hydrolase 25 OS=Arabidopsis thaliana OX=3702 GN=NUDT25 PE=1 SV=1[more]
Q3IZC13.1e-3851.92RNA pyrophosphohydrolase OS=Rhodobacter sphaeroides (strain ATCC 17023 / DSM 158... [more]
B9KN593.1e-3851.92RNA pyrophosphohydrolase OS=Rhodobacter sphaeroides (strain KD131 / KCTC 12085) ... [more]
Match NameE-valueIdentityDescription
A0A6J1HWY84.6e-10190.55nudix hydrolase 26, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111467610 PE... [more]
A0A6J1FZW71.3e-10091.04nudix hydrolase 26, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11144... [more]
A0A1S3BGM32.5e-9989.55nudix hydrolase 26, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489378 PE=3 S... [more]
A0A0A0KRB91.6e-9888.56Nudix hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G61... [more]
A0A6J1DAT22.8e-9887.86nudix hydrolase 26, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111018605... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020476NUDIX hydrolasePRINTSPR00502NUDIXFAMILYcoord: 75..89
score: 34.5
coord: 89..104
score: 49.14
NoneNo IPR availableGENE3D3.90.79.10Nucleoside Triphosphate Pyrophosphohydrolasecoord: 37..200
e-value: 3.0E-53
score: 181.9
NoneNo IPR availablePANTHERPTHR11839UDP/ADP-SUGAR PYROPHOSPHATASEcoord: 20..200
NoneNo IPR availablePANTHERPTHR11839:SF22NUDIX HYDROLASE 26, CHLOROPLASTICcoord: 20..200
IPR000086NUDIX hydrolase domainPFAMPF00293NUDIXcoord: 48..191
e-value: 1.1E-26
score: 93.3
IPR000086NUDIX hydrolase domainPROSITEPS51462NUDIXcoord: 47..194
score: 21.147644
IPR020084NUDIX hydrolase, conserved sitePROSITEPS00893NUDIX_BOXcoord: 80..101
IPR022927RNA pyrophosphohydrolase RppHHAMAPMF_00298Nudix_RppHcoord: 42..201
score: 31.521263
IPR022927RNA pyrophosphohydrolase RppHCDDcd03671Ap4A_hydrolase_plant_likecoord: 47..196
e-value: 6.56831E-75
score: 220.54
IPR015797NUDIX hydrolase-like domain superfamilySUPERFAMILY55811Nudixcoord: 44..199

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Bhi07G000624Bhi07G000624gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi07M000624.exon6Bhi07M000624.exon6exon
Bhi07M000624.exon5Bhi07M000624.exon5exon
Bhi07M000624.exon4Bhi07M000624.exon4exon
Bhi07M000624.exon3Bhi07M000624.exon3exon
Bhi07M000624.exon2Bhi07M000624.exon2exon
Bhi07M000624.exon1Bhi07M000624.exon1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi07M000624.utr3p1Bhi07M000624.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Bhi07M000624cds.Bhi07M000624_6CDS
cds.Bhi07M000624cds.Bhi07M000624_5CDS
cds.Bhi07M000624cds.Bhi07M000624_4CDS
cds.Bhi07M000624cds.Bhi07M000624_3CDS
cds.Bhi07M000624cds.Bhi07M000624_2CDS
cds.Bhi07M000624cds.Bhi07M000624CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi07M000624.utr5p1Bhi07M000624.utr5p1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Bhi07M000624Bhi07M000624-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006753 nucleoside phosphate metabolic process
biological_process GO:0019693 ribose phosphate metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0034432 bis(5'-adenosyl)-pentaphosphatase activity
molecular_function GO:0008893 guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity