Bhi06M000158 (mRNA) Wax gourd (B227) v1

Overview
NameBhi06M000158
TypemRNA
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionTMV resistance protein N-like
Locationchr6: 4005314 .. 4009316 (+)
Sequence length1770
RNA-Seq ExpressionBhi06M000158
SyntenyBhi06M000158
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAAGAGATAAATACATGAAGGAGGTTCAACAATGGCGGAGAGCTTTGACAAAAGTCGGTGATCTCTCTGGAGTAGTTGTAACGAAAGATAGGTGATGTAATTTCAACGTATCATCTTCTGTTTAGGATCTGTTTGGATCGATTAATTTTTTTTTTAAGAAAATCATTTGCGGATTTTCGAAAATAGAAAAATAAGGACAACTATGCATACAAGATGGCAAAACTTAATCTTCTTTTATTATTGAAGTCTATAATTGTCTACTAGTGATAGAAATTGATAGAAGTCTATTATTGATATCATATGATAGATGTTGATAGAAGTCAATGAGTGTCTATCCGTGTTTTTTTTTTTTTTTTGTTATTTTTGTAAATAATTTGACATTTTTTCTATCTGTGAAAATTTTTCAAAATCATTTTTATTTGAACTTTTTCAATAAAAAAAGTTTAAAATACACTTTGAAAAGGTTTCAAGAACTCTATTTTGATTGGTTACCAAATATTTCAATTTTTTTTTTTCTAAAATTACTTATGTTCAAAATTAAACATTTATAAAGTTAAACCAAATATACCCTTAGTTCATCTTATATAAATAGCTCCAGATCCACATTTAAAAGGCGATTTTGAAATTTAATCGAGTGATAGAGTGAGACGTATCTTTGTAATTTAGCCTTTGAACATGCTGAATAAAGAATTTGAGCTACTCGTTTGCACAGTGGATGTAGACTCTTTTCAAATCATTGGTGTCAATCTTCTTTCTGGAACATTTCTTCTGTATTTCTTTTGTTTGTTTGATAATTTTTCATCACACAAAATAGTAAAGTTTAGTTGAGACTCGTGGCATTGTAAGCTGGTAACAAGTGTATGCATGTGTTACGTGCATTTTTAACATGAACTTTAAAATTTGTGTCTAATAAGTTACTTAACCAATTTGAAAATGTTAATAAACTCCTAAAATATAAATTTTAATTGACAATATTACCCTTTGATGTTATATGATTGAATATGCAGCGTTGAAGTGGCTAGCATTGGCAAAATCACAAAGCAATTGCTTGATATTTTGAATCATCAAATGTTAGTAGCTTCAGACAAGGTGGATAGGTTAGTTGATATGGAACGTCGGTTATCCAAGATGGATGAGCTAATTGATTTGGAATCGAATGATGTACGTTTTGTAGGGATAACAGGGATGGGTGGAATTGGTAAAACAACCATCGCTGAAGTTTTTTACGATAAAGTTGCAAATAAGTTTGGAAAAAATCATTGTTTTCTTCGCATTTATGAACGCACTTTAGTCTCACTTCAACAGCAACTTCTTTCCCAACTTCTTCGAACAAAGGACATTGTGATAAACAATGAGAATGAAGGAGCAAGAATGATTGAAAGTTATTTGAAAGGTAAAAAGGTTTTTATTGCTCTTGATGGGGTGAAAGAAATAAGTCAACTAGAAATGTTAGTTGGAAGTCCCATTTGGTTTGGTCAAGGGTCCAAAATCATCATTACAACTAGAAACAGAGATGTTCTTCGTCAACCTAATTACAGAGATATAATGGTAGAATACAATGTGGAGTTTCTTGATTATGAAAGTGCCATGTCACTCTTTTGCAAACATGCCTTTGGATCTGAGTTTCCCAATAGGAATTTTGAGGATTTGTCTAAGGAGATTGTAGAAAGGGTTAAAGGACATCCACAAGCTTTGATACAAATTGGATTGTCTTTGTATGATAAAGGTATAGATATATGGAAGGAAGAATTGAAGAGTCTTGAGAAAGATTACATGAATAAACATCTATTCAAGACATTAAAGATAAGTTTTGATGATCTAGGAAAGACAAGCCAAGAAGTTTTTCTTGATTTGGCATGTTTCTTCAATGGGAAGAAGAAAGAGAAAGTGATTGAAATACTTAAGAGTTTTGATTATAGACCTCATAGCGAATTAAAATTATTGGAAGATAGATGTCTTATTGAAGTTAGACGTGACAACACAATATTTATGCCTAATTGCATTCAAGCTATGGGTCAAGAAATTGAACGTGGAGCTGATAAACGAAGTAGGATTTGGCTTCCAAAAGACGCCATTGATGCATTTGATCAACGACATGTAAGACTAAAATATGTATATTGATTTTCTTTTTTTAAAAAGATATATAAGTTAATTTCTTTCCCTTTTTTTGTTTTGAATAATATTTTTTTATTTCCAGAGAGTAAGGGACATTAAAGGCATAGTGTTGGAAATGGAAGAGAAGCAAGATGAAGTAGAGTTGGAGGGTAAGGTTTTTGAAGATATGACATGTTTAAAAATATTGCAAATTAGCAATGTGAAGGTGATTGGAGACTTCACACATCTCTCAAAACAACTGCGATTGCTCAATTGGCATAGTTATCCCTCAGAATGTTTGCCATTACGTTTTGACTCGAGATATTTATTTCAACTTCTCTTGCCTCTTAGTCAAACTAGGCAACTTTGGAATGGACAAAAGGTTAGTACATCCAAAAATTACTGAAAAAAACCATTTCTTAAATGTTCACGATCAAATACTACTTTGGTCTATATACTTTTGACTTAGTTCATTTAGGTTATTATACTTTTTCTTAAAACAAGTTACTAGAAGAGAAAGGGTCAAGACGTGCACTGGGACAGTAAAAATTGTCATTTGGGAATTTATACTTTGAAAATATTCATTTTTTTATCCTTAATCTATTAAAAAGTAACCGTTTCTGTATTTTTATTTTTTTCAACAATCAAAATTGTCATTTTCAAGGTCCAAATGAATCAAAGTCGGAAGTATCAAGACCAAAATGAACGCAAAAATACAAAGACCAAAGTAGTACTCAAATCTAATGTAATTGTATTGTTAAGAAGTAGTAATATCTCAAAAGATGCACCTGAGCTATGTTTTGGTGACAGGGATTTGAGAAATTGAAGCTTATCAATGTTAGTGATTCAAAGAATTTGCGAGAGACTCCTAATTTTACAAAGGTTCCAAATCTTGAAAGTTTGGTTCTAAGTAATTGTACAAGATTGTGGAAGATTGATTCTTCTATTAGTCGTCTCAATCGTTTGACATTGTTGGATCTAACATGTTGTATTAATCTCAAAAGCCTTCCATCAATTCAAAGCTGCAAGAGCCTCACAACATTAAATTTGGCTGGCTCAGGTCTTGAGTGTCTCGATCAAAAAGGTATTTACTAATTTTTACTAATTTTACATTGTGGTAAATTGAATTATTCCTTTTATCCAACATCTAGGTTTAAATTTTTGGTAAATTACACTTTTAATCTTTGAAGTTGAGTTGAGTCCTTAAATTTTAAAATTGCACAAAAAAATCGTCAACATATCTCAAGAAAAAATGTTGGACGATTTTGGTCCCTCAATCAATATTTCGGTTAACAACTATCAAGATTAGGCTAAGGTGGAATCGTGTTAAACTATAATAATAATAATAATTTGAAAAATCTCAAAAGAAAGGATTTTGAAGTGCGTAAATTATGAATAAGTATTTCAAAAGAAATGATTTAGAAAGTTCTAATCCGTTCTTTAGGCTCTAATGAATTTGAGAATTCCTTTATATATGGATGTACTCAGAGAAATATATGCCTTAAGATATTATAGATCCAAAAAGAACCTAAACTAATCCATTTTCTTCATGGATCCCAGTTATATAAATATGAGGAATATTCTCACATGGTAAGCTATGAAAGAAACATGTTTAGAAAGTTATGAAACTTTTGAGAAATTTAGAATGATTGAAGAAAAATCAGTTTGTGAGTATTGAGAGAGTGAAAAAGTTCTCTACCAAAAGTGTCCATATGTATACTTAACAATACTTTCTTTCTTTCAATAATTGTCTCTATTCTTTTTGTATCAATTTCTTCGACAATATATAACATCTATTATGATAATTTCCTACGATTATTCCTTTTCTTTCTAATATTATCAACTTCATTTTATAATATGTTTTGGTAACCAGGTTTGGAGCACTTTCAAAACGTCTTCCAACTT

mRNA sequence

ATGGAAAGAGATAAATACATGAAGGAGGTTCAACAATGGCGGAGAGCTTTGACAAAAGTCGGTGATCTCTCTGGAGTAGTTGTAACGAAAGATAGCGTTGAAGTGGCTAGCATTGGCAAAATCACAAAGCAATTGCTTGATATTTTGAATCATCAAATGTTAGTAGCTTCAGACAAGGTGGATAGGTTAGTTGATATGGAACGTCGGTTATCCAAGATGGATGAGCTAATTGATTTGGAATCGAATGATGTACGTTTTGTAGGGATAACAGGGATGGGTGGAATTGGTAAAACAACCATCGCTGAAGTTTTTTACGATAAAGTTGCAAATAAGTTTGGAAAAAATCATTGTTTTCTTCGCATTTATGAACGCACTTTAGTCTCACTTCAACAGCAACTTCTTTCCCAACTTCTTCGAACAAAGGACATTGTGATAAACAATGAGAATGAAGGAGCAAGAATGATTGAAAGTTATTTGAAAGGTAAAAAGGTTTTTATTGCTCTTGATGGGGTGAAAGAAATAAGTCAACTAGAAATGTTAGTTGGAAGTCCCATTTGGTTTGGTCAAGGGTCCAAAATCATCATTACAACTAGAAACAGAGATGTTCTTCGTCAACCTAATTACAGAGATATAATGGTAGAATACAATGTGGAGTTTCTTGATTATGAAAGTGCCATGTCACTCTTTTGCAAACATGCCTTTGGATCTGAGTTTCCCAATAGGAATTTTGAGGATTTGTCTAAGGAGATTGTAGAAAGGGTTAAAGGACATCCACAAGCTTTGATACAAATTGGATTGTCTTTGTATGATAAAGGTATAGATATATGGAAGGAAGAATTGAAGAGTCTTGAGAAAGATTACATGAATAAACATCTATTCAAGACATTAAAGATAAGTTTTGATGATCTAGGAAAGACAAGCCAAGAAGTTTTTCTTGATTTGGCATGTTTCTTCAATGGGAAGAAGAAAGAGAAAGTGATTGAAATACTTAAGAGTTTTGATTATAGACCTCATAGCGAATTAAAATTATTGGAAGATAGATGTCTTATTGAAGTTAGACGTGACAACACAATATTTATGCCTAATTGCATTCAAGCTATGGGTCAAGAAATTGAACGTGGAGCTGATAAACGAAGTAGGATTTGGCTTCCAAAAGACGCCATTGATGCATTTGATCAACGACATAGAGTAAGGGACATTAAAGGCATAGTGTTGGAAATGGAAGAGAAGCAAGATGAAGTAGAGTTGGAGGGTAAGGTTTTTGAAGATATGACATGTTTAAAAATATTGCAAATTAGCAATGTGAAGGTGATTGGAGACTTCACACATCTCTCAAAACAACTGCGATTGCTCAATTGGCATAGTTATCCCTCAGAATGTTTGCCATTACGTTTTGACTCGAGATATTTATTTCAACTTCTCTTGCCTCTTAGTCAAACTAGGCAACTTTGGAATGGACAAAAGGGATTTGAGAAATTGAAGCTTATCAATGTTAGTGATTCAAAGAATTTGCGAGAGACTCCTAATTTTACAAAGGTTCCAAATCTTGAAAGTTTGGTTCTAAGTAATTGTACAAGATTGTGGAAGATTGATTCTTCTATTAGTCGTCTCAATCGTTTGACATTGTTGGATCTAACATGTTGTATTAATCTCAAAAGCCTTCCATCAATTCAAAGCTGCAAGAGCCTCACAACATTAAATTTGGCTGGCTCAGGTCTTGAGTGTCTCGATCAAAAAGGTTTGGAGCACTTTCAAAACGTCTTCCAACTT

Coding sequence (CDS)

ATGGAAAGAGATAAATACATGAAGGAGGTTCAACAATGGCGGAGAGCTTTGACAAAAGTCGGTGATCTCTCTGGAGTAGTTGTAACGAAAGATAGCGTTGAAGTGGCTAGCATTGGCAAAATCACAAAGCAATTGCTTGATATTTTGAATCATCAAATGTTAGTAGCTTCAGACAAGGTGGATAGGTTAGTTGATATGGAACGTCGGTTATCCAAGATGGATGAGCTAATTGATTTGGAATCGAATGATGTACGTTTTGTAGGGATAACAGGGATGGGTGGAATTGGTAAAACAACCATCGCTGAAGTTTTTTACGATAAAGTTGCAAATAAGTTTGGAAAAAATCATTGTTTTCTTCGCATTTATGAACGCACTTTAGTCTCACTTCAACAGCAACTTCTTTCCCAACTTCTTCGAACAAAGGACATTGTGATAAACAATGAGAATGAAGGAGCAAGAATGATTGAAAGTTATTTGAAAGGTAAAAAGGTTTTTATTGCTCTTGATGGGGTGAAAGAAATAAGTCAACTAGAAATGTTAGTTGGAAGTCCCATTTGGTTTGGTCAAGGGTCCAAAATCATCATTACAACTAGAAACAGAGATGTTCTTCGTCAACCTAATTACAGAGATATAATGGTAGAATACAATGTGGAGTTTCTTGATTATGAAAGTGCCATGTCACTCTTTTGCAAACATGCCTTTGGATCTGAGTTTCCCAATAGGAATTTTGAGGATTTGTCTAAGGAGATTGTAGAAAGGGTTAAAGGACATCCACAAGCTTTGATACAAATTGGATTGTCTTTGTATGATAAAGGTATAGATATATGGAAGGAAGAATTGAAGAGTCTTGAGAAAGATTACATGAATAAACATCTATTCAAGACATTAAAGATAAGTTTTGATGATCTAGGAAAGACAAGCCAAGAAGTTTTTCTTGATTTGGCATGTTTCTTCAATGGGAAGAAGAAAGAGAAAGTGATTGAAATACTTAAGAGTTTTGATTATAGACCTCATAGCGAATTAAAATTATTGGAAGATAGATGTCTTATTGAAGTTAGACGTGACAACACAATATTTATGCCTAATTGCATTCAAGCTATGGGTCAAGAAATTGAACGTGGAGCTGATAAACGAAGTAGGATTTGGCTTCCAAAAGACGCCATTGATGCATTTGATCAACGACATAGAGTAAGGGACATTAAAGGCATAGTGTTGGAAATGGAAGAGAAGCAAGATGAAGTAGAGTTGGAGGGTAAGGTTTTTGAAGATATGACATGTTTAAAAATATTGCAAATTAGCAATGTGAAGGTGATTGGAGACTTCACACATCTCTCAAAACAACTGCGATTGCTCAATTGGCATAGTTATCCCTCAGAATGTTTGCCATTACGTTTTGACTCGAGATATTTATTTCAACTTCTCTTGCCTCTTAGTCAAACTAGGCAACTTTGGAATGGACAAAAGGGATTTGAGAAATTGAAGCTTATCAATGTTAGTGATTCAAAGAATTTGCGAGAGACTCCTAATTTTACAAAGGTTCCAAATCTTGAAAGTTTGGTTCTAAGTAATTGTACAAGATTGTGGAAGATTGATTCTTCTATTAGTCGTCTCAATCGTTTGACATTGTTGGATCTAACATGTTGTATTAATCTCAAAAGCCTTCCATCAATTCAAAGCTGCAAGAGCCTCACAACATTAAATTTGGCTGGCTCAGGTCTTGAGTGTCTCGATCAAAAAGGTTTGGAGCACTTTCAAAACGTCTTCCAACTT

Protein sequence

MERDKYMKEVQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLDILNHQMLVASDKVDRLVDMERRLSKMDELIDLESNDVRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFLRIYERTLVSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDGVKEISQLEMLVGSPIWFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAFGSEFPNRNFEDLSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMNKHLFKTLKISFDDLGKTSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRRDNTIFMPNCIQAMGQEIERGADKRSRIWLPKDAIDAFDQRHRVRDIKGIVLEMEEKQDEVELEGKVFEDMTCLKILQISNVKVIGDFTHLSKQLRLLNWHSYPSECLPLRFDSRYLFQLLLPLSQTRQLWNGQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLLDLTCCINLKSLPSIQSCKSLTTLNLAGSGLECLDQKGLEHFQNVFQL
Homology
BLAST of Bhi06M000158 vs. TAIR 10
Match: AT1G72840.1 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 275.8 bits (704), Expect = 8.1e-74
Identity = 198/571 (34.68%), Postives = 305/571 (53.42%), Query Frame = 0

Query: 2   ERDKYMKE-VQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLDILNHQMLVASDKV 61
           E D  M+E V +WRRALT+V +LSG   +++ V+ A +  I + +  I +    + S  +
Sbjct: 131 EADPEMEEKVSKWRRALTQVANLSG-KHSRNCVDEADM--IAEVVGGISSRLPRMKSTDL 190

Query: 62  DRLVDMERRLSKMDELIDLESND-VRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFL 121
             LV ME  + KM  L+++   D V  +GI GMGGIGK+TIA+  YD+ + +F   HCFL
Sbjct: 191 INLVGMEAHMMKMTLLLNIGCEDEVHMIGIWGMGGIGKSTIAKCLYDRFSRQF-PAHCFL 250

Query: 122 RIYER--TLVSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDGVKEISQL 181
               +   +  LQ++LLS +L  +D+ + +   G++ I+  L  +KVF+ LD V ++ QL
Sbjct: 251 ENVSKGYDIKHLQKELLSHILYDEDVELWSMEAGSQEIKERLGHQKVFVVLDNVDKVEQL 310

Query: 182 EMLVGSPIWFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAFGSE 241
             L   P WFG GS+IIITTR++ +L      +I   Y V+ LD + A+ +F K AFG  
Sbjct: 311 HGLAKDPSWFGPGSRIIITTRDKGLLNSCGVNNI---YEVKCLDDKDALQVFKKLAFGGR 370

Query: 242 FPNRNFEDLSKEIVERVKGHPQALIQIGLSLYD-KGIDIWKEELKSLEKDYMNKHLFKTL 301
            P+  FE L         G P AL+     L     ID W++EL  LE  +  K++ + L
Sbjct: 371 PPSDGFEQLFIRASRLAHGLPSALVAFASHLSAIVAIDEWEDELALLE-TFPQKNVQEIL 430

Query: 302 KISFDDLGKTSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRRDN 361
           + S+D L +  + VFL +ACFFNG     +   LK+ D R    +  L  +CL+ +  D 
Sbjct: 431 RASYDGLDQYDKTVFLHVACFFNGGHLRYIRAFLKNCDAR----INHLAAKCLVNISIDG 490

Query: 362 TIFMPNCIQAMGQEIER-----GADKRSRIWLPKDAIDAFDQRHRVRDIKGIVLEMEEKQ 421
            I M   +   G+EI R        K+  +W P +     D     R ++G+ L + E  
Sbjct: 491 CISMHILLVQTGREIVRQESDWRPSKQRFLWDPTEIHYVLDSNTGTRRVEGLSLHLCEMA 550

Query: 422 DEVELEGKVF---EDMTCLKILQ-----ISNVKVIGDFTHLSKQLRLLNWHSYPSECLPL 481
           D + L   VF    ++T LK  Q     +SN+++I D   LS+ L+LL+W +YP   LP 
Sbjct: 551 DTLLLRNSVFGPMHNLTFLKFFQHLGGNVSNLQLISDDYVLSRNLKLLHWDAYPLTILPP 610

Query: 482 RFDSRYLFQLLLPLSQTRQLWNGQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSN 541
            F    + +L L  S+   LW+G K    L++++V+ S+NLRE P  +   NLE L+L +
Sbjct: 611 IFRPHTIIELSLRYSKLNSLWDGTKLLPNLRILDVTGSRNLRELPELSTAVNLEELILES 670

Query: 542 CTRLWKIDSSISRLNRLTLLDLTCCINLKSL 555
           CT L +I  SI+RL  L  L++  C  L+ +
Sbjct: 671 CTSLVQIPESINRL-YLRKLNMMYCDGLEGV 688

BLAST of Bhi06M000158 vs. TAIR 10
Match: AT1G72840.2 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 275.8 bits (704), Expect = 8.1e-74
Identity = 198/571 (34.68%), Postives = 305/571 (53.42%), Query Frame = 0

Query: 2   ERDKYMKE-VQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLDILNHQMLVASDKV 61
           E D  M+E V +WRRALT+V +LSG   +++ V+ A +  I + +  I +    + S  +
Sbjct: 131 EADPEMEEKVSKWRRALTQVANLSG-KHSRNCVDEADM--IAEVVGGISSRLPRMKSTDL 190

Query: 62  DRLVDMERRLSKMDELIDLESND-VRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFL 121
             LV ME  + KM  L+++   D V  +GI GMGGIGK+TIA+  YD+ + +F   HCFL
Sbjct: 191 INLVGMEAHMMKMTLLLNIGCEDEVHMIGIWGMGGIGKSTIAKCLYDRFSRQF-PAHCFL 250

Query: 122 RIYER--TLVSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDGVKEISQL 181
               +   +  LQ++LLS +L  +D+ + +   G++ I+  L  +KVF+ LD V ++ QL
Sbjct: 251 ENVSKGYDIKHLQKELLSHILYDEDVELWSMEAGSQEIKERLGHQKVFVVLDNVDKVEQL 310

Query: 182 EMLVGSPIWFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAFGSE 241
             L   P WFG GS+IIITTR++ +L      +I   Y V+ LD + A+ +F K AFG  
Sbjct: 311 HGLAKDPSWFGPGSRIIITTRDKGLLNSCGVNNI---YEVKCLDDKDALQVFKKLAFGGR 370

Query: 242 FPNRNFEDLSKEIVERVKGHPQALIQIGLSLYD-KGIDIWKEELKSLEKDYMNKHLFKTL 301
            P+  FE L         G P AL+     L     ID W++EL  LE  +  K++ + L
Sbjct: 371 PPSDGFEQLFIRASRLAHGLPSALVAFASHLSAIVAIDEWEDELALLE-TFPQKNVQEIL 430

Query: 302 KISFDDLGKTSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRRDN 361
           + S+D L +  + VFL +ACFFNG     +   LK+ D R    +  L  +CL+ +  D 
Sbjct: 431 RASYDGLDQYDKTVFLHVACFFNGGHLRYIRAFLKNCDAR----INHLAAKCLVNISIDG 490

Query: 362 TIFMPNCIQAMGQEIER-----GADKRSRIWLPKDAIDAFDQRHRVRDIKGIVLEMEEKQ 421
            I M   +   G+EI R        K+  +W P +     D     R ++G+ L + E  
Sbjct: 491 CISMHILLVQTGREIVRQESDWRPSKQRFLWDPTEIHYVLDSNTGTRRVEGLSLHLCEMA 550

Query: 422 DEVELEGKVF---EDMTCLKILQ-----ISNVKVIGDFTHLSKQLRLLNWHSYPSECLPL 481
           D + L   VF    ++T LK  Q     +SN+++I D   LS+ L+LL+W +YP   LP 
Sbjct: 551 DTLLLRNSVFGPMHNLTFLKFFQHLGGNVSNLQLISDDYVLSRNLKLLHWDAYPLTILPP 610

Query: 482 RFDSRYLFQLLLPLSQTRQLWNGQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSN 541
            F    + +L L  S+   LW+G K    L++++V+ S+NLRE P  +   NLE L+L +
Sbjct: 611 IFRPHTIIELSLRYSKLNSLWDGTKLLPNLRILDVTGSRNLRELPELSTAVNLEELILES 670

Query: 542 CTRLWKIDSSISRLNRLTLLDLTCCINLKSL 555
           CT L +I  SI+RL  L  L++  C  L+ +
Sbjct: 671 CTSLVQIPESINRL-YLRKLNMMYCDGLEGV 688

BLAST of Bhi06M000158 vs. TAIR 10
Match: AT1G63870.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 270.8 bits (691), Expect = 2.6e-72
Identity = 180/578 (31.14%), Postives = 305/578 (52.77%), Query Frame = 0

Query: 8   KEVQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLDILNHQMLVASDKVDRLVDME 67
           ++ Q W +AL  VG+++G    +   E   I KI + + D LN      S   + +V +E
Sbjct: 138 EDKQNWSKALKDVGNIAGEDFLRWDNEAKMIEKIARDVSDKLN---ATPSRDFNGMVGLE 197

Query: 68  RRLSKMDELIDLESNDVRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFLRIYERTLV 127
             L++M+ L+DL+ + V+ VGI+G  GIGKTTIA     +++NKF +  CF+   + + +
Sbjct: 198 AHLTEMESLLDLDYDGVKMVGISGPAGIGKTTIARALQSRLSNKF-QLTCFVDNLKESFL 257

Query: 128 S------LQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDGVKEISQLEMLV 187
           +      LQ+Q L+++L    I I +      +IE  L  ++V I LD V  I QLE L 
Sbjct: 258 NSLDELRLQEQFLAKVLNHDGIRICHSG----VIEERLCKQRVLIILDDVNHIMQLEALA 317

Query: 188 GSPIWFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAFGSEFPNR 247
               WFG GS+I++TT N+++L+Q    D+   Y+V F   E A  + C++AF     + 
Sbjct: 318 NETTWFGSGSRIVVTTENKEILQQHGINDL---YHVGFPSDEQAFEILCRYAFRKTTLSH 377

Query: 248 NFEDLSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMNKHLFKTLKISFD 307
            FE L++ + +     P  L  +G SL  K  + W+E ++ LE    ++ + + L++ + 
Sbjct: 378 GFEKLARRVTKLCGNLPLGLRVLGSSLRGKNEEEWEEVIRRLETILDHQDIEEVLRVGYG 437

Query: 308 DLGKTSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRRDNTIFMP 367
            L +  Q +FL +A FFN    + V  +    +      LK+L D+ LI +  +  I + 
Sbjct: 438 SLHENEQSLFLHIAVFFNYTDGDLVKAMFTDNNLDIKHGLKILADKSLINISNNREIVIH 497

Query: 368 NCIQAMG-QEIERGADKRSRIWLPKDAI-DAFDQRHRVRDIKGIVLEMEEKQDEVELEGK 427
             +Q  G Q + +    + +I +    I D  +     + + GI  ++    DEV + GK
Sbjct: 498 KLLQQFGRQAVHKEEPWKHKILIHAPEICDVLEYATGTKAMSGISFDI-SGVDEVVISGK 557

Query: 428 VFEDMTCLKILQI------SNVKV-IGDFTHLSKQLRLLNWHSYPSECLPLRFDSRYLFQ 487
            F+ +  L+ L++       N +V I + T   ++LRLL+W +YP + LP  F  +YL +
Sbjct: 558 SFKRIPNLRFLKVFKSRDDGNDRVHIPEETEFPRRLRLLHWEAYPCKSLPPTFQPQYLVE 617

Query: 488 LLLPLSQTRQLWNGQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDS 547
           L +P SQ  +LW G +    LK +N+  S++L+E P+ +   NLE + LS C  L +I S
Sbjct: 618 LYMPSSQLEKLWEGTQRLTHLKKMNLFASRHLKELPDLSNATNLERMDLSYCESLVEIPS 677

Query: 548 SISRLNRLTLLDLTCCINLKSLPSIQSCKSLTTLNLAG 571
           S S L++L  L++  CINL+ +P+  +  SL T+N+ G
Sbjct: 678 SFSHLHKLEWLEMNNCINLQVIPAHMNLASLETVNMRG 703

BLAST of Bhi06M000158 vs. TAIR 10
Match: AT4G19510.2 (Disease resistance protein (TIR-NBS-LRR class) )

HSP 1 Score: 268.1 bits (684), Expect = 1.7e-71
Identity = 192/587 (32.71%), Postives = 304/587 (51.79%), Query Frame = 0

Query: 8   KEVQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLDILNHQMLVASDKVDRLVDME 67
           +++++ + AL    ++ G V  ++S E   + +I K    +LN   L      D L  +E
Sbjct: 139 QKIEELKVALKTASNIRGFVYPENSSEPDFLDEIVKNTFRMLNE--LSPCVIPDDLPGIE 198

Query: 68  RRLSKMDELIDLESND-VRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFLRIYERT- 127
            R  ++++L+  ++++ VR VG+ GM GIGKTT+A++ Y +   +F        I + + 
Sbjct: 199 SRSKELEKLLMFDNDECVRVVGVLGMTGIGKTTVADIVYKQNFQRFDGYEFLEDIEDNSK 258

Query: 128 ---LVSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDGVKEISQLEMLVG 187
              L  L Q+LL +LL  +++ +  +       E++L+ KK+FI LD V E  Q+E L+G
Sbjct: 259 RYGLPYLYQKLLHKLLDGENVDVRAQGRP----ENFLRNKKLFIVLDNVTEEKQIEYLIG 318

Query: 188 SPIWFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAFGSEFPNRN 247
               + QGS+I+I TR++ +L+    ++    Y V  L+   AM LFC   FG+ +P   
Sbjct: 319 KKNVYRQGSRIVIITRDKKLLQ----KNADATYVVPRLNDREAMELFCLQVFGNHYPTEE 378

Query: 248 FEDLSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMNKHLFKTLKISFDD 307
           F DLS + V   KG P AL  +G  L    I+ WK++L+ L+ +  +K L K LK S+  
Sbjct: 379 FVDLSNDFVCYAKGLPLALKLLGKGLLTHDINYWKKKLEFLQVN-PDKELQKELKSSYKA 438

Query: 308 LGKTSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRRDNTIFMPN 367
           L    + VFLD+ACFF  +K + V  ILKS D      ++ LE++CL+ +  D  I M +
Sbjct: 439 LDDDQKSVFLDIACFFRSEKADFVSSILKSDDIDAKDVMRELEEKCLVTISYDR-IEMHD 498

Query: 368 CIQAMGQEIE-----RGADKRSRIWLPKDAIDAFDQRHRVRDIKGIVLEMEEKQDEVELE 427
            + AMG+EI      R A +R R+W  KD  D  +       ++GI L M E +  ++L 
Sbjct: 499 LLHAMGKEIGKEKSIRKAGERRRLWNHKDIRDILEHNTGTECVRGIFLNMSEVR-RIKLF 558

Query: 428 GKVFEDMTCLKILQ--------------ISNVKVIGDFTHLSKQLRLLNWHSYPSECLPL 487
              F  ++ LK L+              I     + D  H   +L  L+W  YP +CLP 
Sbjct: 559 PAAFTMLSKLKFLKFHSSHCSQWCDNDHIFQCSKVPD--HFPDELVYLHWQGYPYDCLPS 618

Query: 488 RFDSRYLFQLLLPLSQTRQLWNGQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSN 547
            FD + L  L L  S  +QLW  +K  E L+ +++  SK+L      ++  NLE L L  
Sbjct: 619 DFDPKELVDLSLRYSHIKQLWEDEKNTESLRWVDLGQSKDLLNLSGLSRAKNLERLDLEG 678

Query: 548 CTRLWKIDSSISRLNRLTLLDLTCCINLKSLPSIQSCKSLTTLNLAG 571
           CT L  +  S+ ++N L  L+L  C +L+SLP     KSL TL L+G
Sbjct: 679 CTSL-DLLGSVKQMNELIYLNLRDCTSLESLPKGFKIKSLKTLILSG 709

BLAST of Bhi06M000158 vs. TAIR 10
Match: AT5G17970.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 266.5 bits (680), Expect = 4.9e-71
Identity = 176/591 (29.78%), Postives = 298/591 (50.42%), Query Frame = 0

Query: 8   KEVQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLDILNHQMLVASDKVDRLVDME 67
           +E Q+WR+ALT VG+++G   +K   +   I KI   + + L      +S   + L+ +E
Sbjct: 137 EERQRWRQALTNVGNIAGECSSKWDNDAKMIEKIVAYVSEEL--FCFTSSTDFEDLLGLE 196

Query: 68  RRLSKMDELIDLESNDVRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFLRIYERTLV 127
             ++ +  ++ LESN+V+ +G+ G  GIGKTTI  + Y+++++    +       E    
Sbjct: 197 AHVANLKSMLGLESNEVKMIGVWGPAGIGKTTITRILYNQLSSSNDDDFQLFIFMENVKG 256

Query: 128 S--------------LQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDGVKE 187
           S              L+++ LS++   + I +++      + +  LK +K  I LD V E
Sbjct: 257 SYRRKEIDGYSMKLHLRERFLSEITTQRKIKVSH----LGVAQERLKNQKALIVLDDVDE 316

Query: 188 ISQLEMLVGSPIWFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHA 247
           + QL  L     W G G++I++TT +R +L+      +   Y V++   + A+ + C+ A
Sbjct: 317 LEQLRALADQTQWVGNGTRILVTTEDRQLLKAHGITHV---YEVDYPSRDEALKILCQCA 376

Query: 248 FGSEFPNRNFEDLSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMNKHLF 307
           FG       + DL+ E+VE     P  L  +G SL       W   L  L +  +N  + 
Sbjct: 377 FGKNSAPEGYNDLAVEVVELAGYLPLGLSVLGASLRGMSKKEWINALPRL-RTSLNGKIE 436

Query: 308 KTLKISFDDLGKTSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVR 367
           K L++ ++ L +  + +FL +AC FNGK  ++V  +L          LK+L DR LI + 
Sbjct: 437 KLLRVCYEGLDEKDKAIFLHIACLFNGKNVDRVKLLLAKSALDVEFGLKVLVDRSLIHID 496

Query: 368 RDNTIFMPNCIQAMGQEIERG-----ADKRSRIWLPKDAIDAFDQRHRVRDIKGIVLEME 427
            D  I M   +Q +G+EI RG       KR  +    +  D          + GI L+M 
Sbjct: 497 ADGYIVMHCLLQQLGKEITRGQCLDEPGKRKFLVDSLEISDVLADETGTETVLGISLDMS 556

Query: 428 EKQDEVELEGKVFEDMTCLKIL--------QISNVKVIGDFTHLSKQLRLLNWHSYPSEC 487
           E +D+V +  K FE M  L+ L        +   + +     +L ++LRLL+W SYP +C
Sbjct: 557 EIEDQVYVSEKAFEKMPNLQFLWLYKNFPDEAVKLYLPHGLDYLPRKLRLLHWDSYPKKC 616

Query: 488 LPLRFDSRYLFQLLLPLSQTRQLWNGQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLV 547
           LP +F   +L +L +  S+  +LW G +  + LK +++S S  +++ PN ++  NLE L 
Sbjct: 617 LPSKFRPEFLVELTMRDSKLEKLWEGIQPLKSLKRMDLSASTKIKDIPNLSRATNLEKLY 676

Query: 548 LSNCTRLWKIDSS-ISRLNRLTLLDLTCCINLKSLPSIQSCKSLTTLNLAG 571
           L  C  L  + SS +  L++L +LD++CCI LKSLP   + KSL+ LN+ G
Sbjct: 677 LRFCKNLVIVPSSCLQNLHKLKVLDMSCCIKLKSLPDNINLKSLSVLNMRG 717

BLAST of Bhi06M000158 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 2.3e-81
Identity = 217/617 (35.17%), Postives = 320/617 (51.86%), Query Frame = 0

Query: 4   DKYMKEVQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLDILNHQMLVASDKVDRL 63
           + +  ++ +WR ALT+  +LSG  +  D  E   I +IT  +   L  + L   D    L
Sbjct: 140 ENWKDKIPRWRTALTEAANLSGWHLLDDRYESNQIKEITNSIFRQLKCKRL---DVGANL 199

Query: 64  VDMERRLSKMDELIDLESNDVRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFLRIYE 123
           V ++  + +M   + LES+DVR VGI G+GGIGKTTIA+V Y++++ +F        I E
Sbjct: 200 VGIDSHVKEMILRLHLESSDVRMVGIYGVGGIGKTTIAKVIYNELSCEFEYMSFLENIRE 259

Query: 124 ----RTLVSLQQQLLSQLLRTKDIV-INNENEGARMIESYLKGKKVFIALDGVKEISQLE 183
               + L  LQ QLL  +L  +    I++    A MI+  L  ++VFI LD V ++SQLE
Sbjct: 260 GSNPQVLFHLQNQLLGDILEGEGSQNISSVAHRASMIKDILLSRRVFIVLDDVDDLSQLE 319

Query: 184 MLVGSPIWFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAFGSEF 243
            L+G   W G+GS++IITTRN+ VL      D+   Y VE L++E A  LF  +AF    
Sbjct: 320 YLLGHREWLGEGSRVIITTRNKHVLAVQEVDDL---YEVEGLNFEEACELFSLYAFKQNL 379

Query: 244 PNRNFEDLSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMNKHLFKTLKI 303
           P  ++ +L+  +V   +G P AL  +G  L  K I  W+ ELK L+ +     + K LK 
Sbjct: 380 PKSDYRNLTCRVVGYCQGLPLALKVLGSLLCKKTIPQWEGELKKLDSE-PKADIHKVLKR 439

Query: 304 SFDDLGKTSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRRDNTI 363
           S+D L +  + +FLDLACFF G+ ++ V+ IL   D+   + +  L D CLI +   N I
Sbjct: 440 SYDGLDRIDKNIFLDLACFFKGEGRDFVLRILDGCDFPAETGISNLNDLCLITLPY-NQI 499

Query: 364 FMPNCIQAMGQEIER-----GADKRSRIWLPKDAIDAFDQRHRVRDIKGIVLEMEEKQDE 423
            M + IQ MG EI R       +K SR+W P D   A      ++ ++ + L++  K   
Sbjct: 500 CMHDLIQQMGWEIVRENFPVEPNKWSRLWDPCDFERALTADEGIKSVETMSLDL-SKLKR 559

Query: 424 VELEGKVFEDMTCLKILQI----------------------------SNVKVIGDFTHLS 483
           V     VF  MT L++L++                            S +++   F   S
Sbjct: 560 VCSNSNVFAKMTKLRLLKVYSSSDIDSAHGDSDEDIEEVYDVVMKDASKMQLGQSFKFPS 619

Query: 484 KQLRLLNWHSYPSECLPLRFDSRYLFQLLLPLSQTRQLWNGQKGFEKLKLINVSDSKNLR 543
            +LR L W  YP + LPL FD   L +L L  S  +QLW G K  E+LK+I++S S+ L 
Sbjct: 620 YELRYLRWDGYPLDSLPLNFDGGKLVELHLKCSNIKQLWQGHKDLERLKVIDLSYSRKLS 679

Query: 544 ETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLLDLTCCINLKSLP-SIQSCKSLT 581
           +   F+ +PNLE L LS C  L  I  S+  + +LT L L  C  LK+LP SI   +SL 
Sbjct: 680 QMSEFSSMPNLERLCLSGCVSLIDIHPSVGNMKKLTTLSLRSCNKLKNLPDSIGDLESLE 739

BLAST of Bhi06M000158 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 7.7e-77
Identity = 213/613 (34.75%), Postives = 317/613 (51.71%), Query Frame = 0

Query: 9   EVQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLDILNHQMLVASDKVDRLVDMER 68
           ++ +WR ALT+  +LSG  + +D  E   I +IT  +   L  + L   D    LV ++ 
Sbjct: 151 KIPRWRTALTEAANLSGWPL-QDGYESNQIKEITDSIFRRLKCKRL---DAGANLVGIDS 210

Query: 69  RLSKMDELIDLESNDVRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFLRIYER---- 128
            + +M   + +ES+DVR VG+ G+GGIGKTTIA+V Y++++ +F        I E+    
Sbjct: 211 HVKEMIWRLHMESSDVRMVGMYGVGGIGKTTIAKVIYNELSREFEYMSFLENIREKFNTQ 270

Query: 129 TLVSLQQQLLSQLLRTKDIV-INNENEGARMIESYLKGKKVFIALDGVKEISQLEMLVGS 188
            +  LQ QLL  +L+ +    IN+   GA MI+  L  K VFI LD V + SQLE L+  
Sbjct: 271 GVSPLQNQLLDDILKGEGSQNINSVAHGASMIKDILSSKIVFIVLDDVDDQSQLEYLLRH 330

Query: 189 PIWFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAFGSEFPNRNF 248
             W G+GS++IITTRN+ VL      D+   Y V+ L++E A  LF  +AF    P  ++
Sbjct: 331 REWLGEGSRVIITTRNKHVLDVQKVDDL---YEVKGLNFEEACELFSLYAFEQNLPKSDY 390

Query: 249 EDLSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMNKHLFKTLKISFDDL 308
            +LS  +V   +G P AL  +G  L  K I  W+ EL+ L+++     +   LK S+D L
Sbjct: 391 RNLSHRVVGYCQGLPLALKVLGCLLLKKTIPEWESELRKLDRE-PEAEILSVLKRSYDGL 450

Query: 309 GKTSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRRDNTIFMPNC 368
           G+T + +FLD+ACFF G+ ++ V +IL + D+     +K L D+CLI ++  N I M + 
Sbjct: 451 GRTEKSIFLDVACFFKGEDRDFVSKILDACDFHAEIGIKNLNDKCLITLQY-NRIRMHDL 510

Query: 369 IQAMGQEIER-----GADKRSRIWLPKDAIDAFDQRHRVRDIKGIVLEMEEKQDEVELEG 428
           IQ MG EI R       +K SR+W   D   A      ++ ++ I L++  K   V    
Sbjct: 511 IQQMGWEIVREKFPDEPNKWSRLWDTCDFERALTAYKGIKRVETISLDL-SKLKRVCSNS 570

Query: 429 KVFEDMTCLKILQI-----------------------------SNVKVIGDFTHLSKQLR 488
             F  MT L++L++                             S +++   F   S +LR
Sbjct: 571 NAFAKMTRLRLLKVQSSLDIDFEPEYIDADDKVELYDVVMKNASKMRLGRGFKFPSYELR 630

Query: 489 LLNWHSYPSECLPLRFDSRYLFQLLLPLSQTRQLWNGQKGFEKLKLINVSDSKNLRETPN 548
            L W  YP + LP  FD   L +L L  S  +QL  G K  E LK+I++S S+ L +   
Sbjct: 631 YLRWDGYPLDFLPSNFDGGKLVELHLKCSNIKQLRLGNKDLEMLKVIDLSYSRKLSQMSE 690

Query: 549 FTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLLDLTCCINLKSLP-SIQSCKSLTTLNL 581
           F+ +PNLE L L  C  L  I  S+  + +LT L L  C  LK+LP SI   +SL  L+L
Sbjct: 691 FSSMPNLERLFLRGCVSLIDIHPSVGNMKKLTTLSLKSCKKLKNLPDSIGDLESLEILDL 750

BLAST of Bhi06M000158 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 277.3 bits (708), Expect = 3.9e-73
Identity = 191/580 (32.93%), Postives = 301/580 (51.90%), Query Frame = 0

Query: 2   ERDKYMKEVQQWRRALTKVGDLSG--VVVTKDSVEVASIGKITKQLLDILNHQMLVASDK 61
           +RDK    V +WR ALTKV ++SG  +  T +  E   I +I K + D     + + +  
Sbjct: 134 DRDK----VLRWREALTKVANISGHDLRNTYNGDESKCIQQILKDIFDKFCFSISITNRD 193

Query: 62  VDRLVDMERRLSKMDELIDLESNDVRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFL 121
              LV +E ++ K+  L+ ++   VR VGI GMGG+GKTT A   +++    F ++ CFL
Sbjct: 194 ---LVGIESQIKKLSSLLRMDLKGVRLVGIWGMGGVGKTTAARALFNRYYQNF-ESACFL 253

Query: 122 R-----IYERTLVSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDGVKEI 181
                 +   TL+ LQ+ LLS+LL+ + +   +  E   +++  L  KKV + LD V   
Sbjct: 254 EDVKEYLQHHTLLYLQKTLLSKLLKVEFVDCTDTEEMCVILKRRLCSKKVLVVLDDVNHN 313

Query: 182 SQLEMLVGSPIWFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAF 241
            QL+ LVG+  WFG GS+I+ITTR+  +L+     D+   Y ++ L+ + A+ LF  HAF
Sbjct: 314 DQLDKLVGAEDWFGSGSRIVITTRDMKLLKN---HDVHETYEIKVLEKDEAIELFNLHAF 373

Query: 242 GSEFPNRNFEDLSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMNKHLFK 301
               P + F++L   +V+   G P AL  +G  LY + +D+W   +  L KD     +  
Sbjct: 374 KRSSPEKEFKELLNLVVDYTGGLPLALKVLGSLLYKEDLDVWISTIDRL-KDNPEGEIMA 433

Query: 302 TLKISFDDLGKTSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRR 361
           TLKISFD L    + +FLD+ACFF G  +  +  +  +  + P   +K L ++ LI +  
Sbjct: 434 TLKISFDGLRDYEKSIFLDIACFFRGYNQRDMTALFHASGFHPVLGVKTLVEKSLIFILE 493

Query: 362 DNTIFMPNCIQAMGQEIERGADKRSRIWLPKDAIDAFDQRHRVRDIKGIVLEMEEKQDEV 421
           D  I M + +Q MG++I        RI+ P+D  DA     R   I+G++L   E+ +E 
Sbjct: 494 DK-IQMHDLMQEMGRQIAVQESPMRRIYRPEDVKDACIGDMRKEAIEGLLLTEPEQFEEG 553

Query: 422 ELE----GKVFEDMTCLKIL--QISNVKVIGDFTHLSKQLRLLNWHSYPSECLPLRFDSR 481
           ELE     +  +    L+IL  +  N        +L   L  L W +Y S   P  F+  
Sbjct: 554 ELEYMYSAEALKKTRRLRILVKEYYNRGFDEPVAYLPNSLLWLEWRNYSSNSFPSNFEPS 613

Query: 482 YLFQLLLPLSQTRQLWNGQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSNCTRLW 541
            L  L +  S   +LWNG K    L  +++S    L +TP+F  + NLE L+LS+C  L 
Sbjct: 614 KLVYLTMKGSSIIELWNGAKRLAFLTTLDLSYCHKLIQTPDFRMITNLERLILSSCDALV 673

Query: 542 KIDSSISRLNRLTLLDLTCCINLKSLPSIQSCKSLTTLNL 569
           ++  S+  L  L LL++  CI+L+ LP+I   + L  L+L
Sbjct: 674 EVHPSVGFLKNLILLNMDHCISLERLPAIIQSECLEVLDL 700

BLAST of Bhi06M000158 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 7.4e-72
Identity = 186/571 (32.57%), Postives = 296/571 (51.84%), Query Frame = 0

Query: 10  VQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLDILNHQMLVASDKVDRLVDMERR 69
           +Q+WR AL +  +L G    +D  +   I +I  Q+   L     ++   +  +V ++  
Sbjct: 138 IQRWRIALNEAANLKGSCDNRDKTDADCIRQIVDQISSKL---CKISLSYLQNIVGIDTH 197

Query: 70  LSKMDELIDLESNDVRFVGITGMGGIGKTTIAEVFYDKVANKFGKNH-----CFLRIY-- 129
           L K++ L+++  N VR +GI GMGG+GKTTIA   +D +  +   ++     CFL+    
Sbjct: 198 LEKIESLLEIGINGVRIMGIWGMGGVGKTTIARAIFDTLLGRMDSSYQFDGACFLKDIKE 257

Query: 130 -ERTLVSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDGV-KEISQLEML 189
            +R + SLQ  LLS+LLR K    NNE +G   + S L+ KKV I LD +  +   LE L
Sbjct: 258 NKRGMHSLQNALLSELLREK-ANYNNEEDGKHQMASRLRSKKVLIVLDDIDNKDHYLEYL 317

Query: 190 VGSPIWFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAFGSEFPN 249
            G   WFG GS+IIITTR++ ++ +    DI+  Y V  L    ++ LF +HAFG E PN
Sbjct: 318 AGDLDWFGNGSRIIITTRDKHLIEK---NDII--YEVTALPDHESIQLFKQHAFGKEVPN 377

Query: 250 RNFEDLSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMNKHLFKTLKISF 309
            NFE LS E+V   KG P AL   G  L++  +  WK  ++ + K+     +   LKIS+
Sbjct: 378 ENFEKLSLEVVNYAKGLPLALKVWGSLLHNLRLTEWKSAIEHM-KNNSYSGIIDKLKISY 437

Query: 310 DDLGKTSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRRDNTIFM 369
           D L    QE+FLD+ACF  G++K+ +++IL+S        L++L D+ L+ +   N + M
Sbjct: 438 DGLEPKQQEMFLDIACFLRGEEKDYILQILESCHIGAEYGLRILIDKSLVFISEYNQVQM 497

Query: 370 PNCIQAMGQEI---ERGADKRSRIWLPKDAIDAFDQRHRVRDIKGIVLEMEEKQDEVELE 429
            + IQ MG+ I   ++   +RSR+WL K+  +          ++ I   +      +   
Sbjct: 498 HDLIQDMGKYIVNFQKDPGERSRLWLAKEVEEVMSNNTGTMAMEAI--WVSSYSSTLRFS 557

Query: 430 GKVFEDMTCLKILQISNVKVIGDFTHLSKQLRLLNWHSYPSECLPLRFDSRYLFQLLLPL 489
            +  ++M  L++  +          +L   LR     +YP E  P  F+ + L  L L  
Sbjct: 558 NQAVKNMKRLRVFNMGRSSTHYAIDYLPNNLRCFVCTNYPWESFPSTFELKMLVHLQLRH 617

Query: 490 SQTRQLWNGQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRL 549
           +  R LW   K    L+ I++S SK L  TP+FT +PNLE + L  C+ L ++  S+   
Sbjct: 618 NSLRHLWTETKHLPSLRRIDLSWSKRLTRTPDFTGMPNLEYVNLYQCSNLEEVHHSLGCC 677

Query: 550 NRLTLLDLTCCINLKSLPSIQSCKSLTTLNL 569
           +++  L L  C +LK  P + + +SL  L L
Sbjct: 678 SKVIGLYLNDCKSLKRFPCV-NVESLEYLGL 695

BLAST of Bhi06M000158 vs. ExPASy Swiss-Prot
Match: F4JT80 (Disease resistance protein RPP2B OS=Arabidopsis thaliana OX=3702 GN=RPP2B PE=1 SV=2)

HSP 1 Score: 268.1 bits (684), Expect = 2.4e-70
Identity = 192/587 (32.71%), Postives = 304/587 (51.79%), Query Frame = 0

Query: 8   KEVQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLDILNHQMLVASDKVDRLVDME 67
           +++++ + AL    ++ G V  ++S E   + +I K    +LN   L      D L  +E
Sbjct: 139 QKIEELKVALKTASNIRGFVYPENSSEPDFLDEIVKNTFRMLNE--LSPCVIPDDLPGIE 198

Query: 68  RRLSKMDELIDLESND-VRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFLRIYERT- 127
            R  ++++L+  ++++ VR VG+ GM GIGKTT+A++ Y +   +F        I + + 
Sbjct: 199 SRSKELEKLLMFDNDECVRVVGVLGMTGIGKTTVADIVYKQNFQRFDGYEFLEDIEDNSK 258

Query: 128 ---LVSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDGVKEISQLEMLVG 187
              L  L Q+LL +LL  +++ +  +       E++L+ KK+FI LD V E  Q+E L+G
Sbjct: 259 RYGLPYLYQKLLHKLLDGENVDVRAQGRP----ENFLRNKKLFIVLDNVTEEKQIEYLIG 318

Query: 188 SPIWFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAFGSEFPNRN 247
               + QGS+I+I TR++ +L+    ++    Y V  L+   AM LFC   FG+ +P   
Sbjct: 319 KKNVYRQGSRIVIITRDKKLLQ----KNADATYVVPRLNDREAMELFCLQVFGNHYPTEE 378

Query: 248 FEDLSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMNKHLFKTLKISFDD 307
           F DLS + V   KG P AL  +G  L    I+ WK++L+ L+ +  +K L K LK S+  
Sbjct: 379 FVDLSNDFVCYAKGLPLALKLLGKGLLTHDINYWKKKLEFLQVN-PDKELQKELKSSYKA 438

Query: 308 LGKTSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRRDNTIFMPN 367
           L    + VFLD+ACFF  +K + V  ILKS D      ++ LE++CL+ +  D  I M +
Sbjct: 439 LDDDQKSVFLDIACFFRSEKADFVSSILKSDDIDAKDVMRELEEKCLVTISYDR-IEMHD 498

Query: 368 CIQAMGQEIE-----RGADKRSRIWLPKDAIDAFDQRHRVRDIKGIVLEMEEKQDEVELE 427
            + AMG+EI      R A +R R+W  KD  D  +       ++GI L M E +  ++L 
Sbjct: 499 LLHAMGKEIGKEKSIRKAGERRRLWNHKDIRDILEHNTGTECVRGIFLNMSEVR-RIKLF 558

Query: 428 GKVFEDMTCLKILQ--------------ISNVKVIGDFTHLSKQLRLLNWHSYPSECLPL 487
              F  ++ LK L+              I     + D  H   +L  L+W  YP +CLP 
Sbjct: 559 PAAFTMLSKLKFLKFHSSHCSQWCDNDHIFQCSKVPD--HFPDELVYLHWQGYPYDCLPS 618

Query: 488 RFDSRYLFQLLLPLSQTRQLWNGQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSN 547
            FD + L  L L  S  +QLW  +K  E L+ +++  SK+L      ++  NLE L L  
Sbjct: 619 DFDPKELVDLSLRYSHIKQLWEDEKNTESLRWVDLGQSKDLLNLSGLSRAKNLERLDLEG 678

Query: 548 CTRLWKIDSSISRLNRLTLLDLTCCINLKSLPSIQSCKSLTTLNLAG 571
           CT L  +  S+ ++N L  L+L  C +L+SLP     KSL TL L+G
Sbjct: 679 CTSL-DLLGSVKQMNELIYLNLRDCTSLESLPKGFKIKSLKTLILSG 709

BLAST of Bhi06M000158 vs. ExPASy TrEMBL
Match: A0A1S3C9Z0 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498649 PE=4 SV=1)

HSP 1 Score: 935.6 bits (2417), Expect = 9.8e-269
Identity = 475/588 (80.78%), Postives = 527/588 (89.63%), Query Frame = 0

Query: 1   MERDKYMKEVQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLD-ILNHQMLVASDK 60
           MERDKYM+EVQQWRRA TKVGDL+GVVVTKDSVEVASIGKIT QLLD +L+HQ LV  D+
Sbjct: 129 MERDKYMEEVQQWRRAFTKVGDLTGVVVTKDSVEVASIGKITNQLLDMLLHHQKLVPWDE 188

Query: 61  VDRLVDMERRLSKMDELIDLESNDVRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFL 120
           + +LVD+ER+L KM++L DLE N VRF+GI GMGGIGKTTIAEVFYD+VA  FGKN CFL
Sbjct: 189 LTKLVDIERQLFKMEKLNDLEPNVVRFIGIIGMGGIGKTTIAEVFYDRVARIFGKNRCFL 248

Query: 121 RIYER-TLVSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDGVKEISQLE 180
           RIYE  TL+SLQQQLLSQLL+TKD++INNENEGARMI S LK K+V I LDGVKE SQLE
Sbjct: 249 RIYEHTTLLSLQQQLLSQLLQTKDLIINNENEGARMIGSRLKDKRVLIVLDGVKEKSQLE 308

Query: 181 MLVGSPIWFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAFGSEF 240
            LVG+P WFG GSKIIITTRNRDVLRQPNY+D MVEY++EFLD +SAM+LFCKHAFG  F
Sbjct: 309 QLVGNPNWFGSGSKIIITTRNRDVLRQPNYKDKMVEYSMEFLDTKSAMTLFCKHAFGCGF 368

Query: 241 PNRNFEDLSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMNKHLFKTLKI 300
           P++NFED SKEIVERVKGHPQALIQIG SLYDKGI+IWKEELKSLE+DY N  +FKTLKI
Sbjct: 369 PSKNFEDFSKEIVERVKGHPQALIQIGSSLYDKGIEIWKEELKSLEEDY-NNRIFKTLKI 428

Query: 301 SFDDLGKTSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRRDNTI 360
           SFDDL KTSQEVFLDLACFFN K KEKVIEILKSFDYRPHSE++LL+DRCLIEVR DNTI
Sbjct: 429 SFDDLEKTSQEVFLDLACFFNEKTKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRSDNTI 488

Query: 361 FMPNCIQAMGQEIERGADKRSRIWLPKDAIDAFDQRHRVRDIKGIVLEMEEKQDEVELEG 420
           FMP CIQ MGQ+IER ADKRSRIW+PKDA D FD+ HRV+DIKG+VL++EEKQDE+ELEG
Sbjct: 489 FMPKCIQTMGQQIEREADKRSRIWIPKDAQDVFDEPHRVKDIKGVVLKLEEKQDEIELEG 548

Query: 421 KVFEDMTCLKILQISNVKVIGDFTHLSKQLRLLNWHSYPSECLPLRFDSRYLFQLLLPLS 480
           KVFEDM  LKIL+I NV+V GDFTHLSKQLRLLNWHSYPS+CLPL F+SRYLFQLLLPLS
Sbjct: 549 KVFEDMRSLKILEIGNVEVSGDFTHLSKQLRLLNWHSYPSQCLPLSFESRYLFQLLLPLS 608

Query: 481 QTRQLWNGQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLN 540
           QTRQLWNGQKGFEKLK+INVS SKNLRETPNFTKVPNLESL LSNCTRLWKIDSSISRLN
Sbjct: 609 QTRQLWNGQKGFEKLKVINVSGSKNLRETPNFTKVPNLESLDLSNCTRLWKIDSSISRLN 668

Query: 541 RLTLLDLTCCINLKSLPSIQSCKSLTTLNLAGSGLECLDQKGLEHFQN 587
           RLTLLD+TCCINLK+L   +SCKSL T+N  GSGLE   +KGL++FQN
Sbjct: 669 RLTLLDITCCINLKNLSFSRSCKSLITINYVGSGLE---EKGLDNFQN 712

BLAST of Bhi06M000158 vs. ExPASy TrEMBL
Match: Q5DMW2 (MRGH9 OS=Cucumis melo OX=3656 GN=MRGH9 PE=4 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 7.8e-266
Identity = 471/586 (80.38%), Postives = 523/586 (89.25%), Query Frame = 0

Query: 1   MERDKYMKEVQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLD-ILNHQMLVASDK 60
           MERDKYM+EVQQWRRA TKVGDL+GVVVTKDSVEVASIGKIT QLLD +L+HQ LV  D+
Sbjct: 129 MERDKYMEEVQQWRRAFTKVGDLTGVVVTKDSVEVASIGKITNQLLDMLLHHQKLVPWDE 188

Query: 61  VDRLVDMERRLSKMDELIDLESNDVRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFL 120
           + +LVD+ER+L KM++L DLE N VRF+GI GMGGIGKTTIAEVFYD+VA  FGKN CFL
Sbjct: 189 LTKLVDIERQLFKMEKLNDLEPNVVRFIGIIGMGGIGKTTIAEVFYDRVARIFGKNRCFL 248

Query: 121 RIYER-TLVSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDGVKEISQLE 180
           RIYE  TL+SLQQQLLSQLL+TKD++INNENEGARMI S LK K+V I LDGVKE SQL+
Sbjct: 249 RIYEHTTLLSLQQQLLSQLLQTKDLIINNENEGARMIGSRLKDKRVLIVLDGVKEKSQLD 308

Query: 181 MLVGSPIWFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAFGSEF 240
            LVG+P WFG GSKIIITTRNRDVLRQPNY+D MVEY++EFLD +SAM+LFCKHAFG  F
Sbjct: 309 QLVGNPNWFGSGSKIIITTRNRDVLRQPNYKDKMVEYSMEFLDTKSAMTLFCKHAFGCGF 368

Query: 241 PNRNFEDLSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMNKHLFKTLKI 300
           P++NFED SKEIVERVKGHPQALIQIG SLYDKGI+IWKEELKSLE+DY N  +FKTLKI
Sbjct: 369 PSKNFEDFSKEIVERVKGHPQALIQIGSSLYDKGIEIWKEELKSLEEDY-NNRIFKTLKI 428

Query: 301 SFDDLGKTSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRRDNTI 360
           SFDDL KTSQEVFLDLACFFN K KEKVIEILKSFDYRPHSE++LL+DRCLIEVR DNTI
Sbjct: 429 SFDDLEKTSQEVFLDLACFFNEKTKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRSDNTI 488

Query: 361 FMPNCIQAMGQEIERGADKRSRIWLPKDAIDAFDQRHRVRDIKGIVLEMEEKQDEVELEG 420
           FMP CIQ MGQ+IER ADKRSRIW+PKDA D FD+ HRV+DIKG+VL++EEKQDE+ELEG
Sbjct: 489 FMPKCIQTMGQQIEREADKRSRIWIPKDAQDVFDEPHRVKDIKGVVLKLEEKQDEIELEG 548

Query: 421 KVFEDMTCLKILQISNVKVIGDFTHLSKQLRLLNWHSYPSECLPLRFDSRYLFQLLLPLS 480
           KVFEDM  LKIL+I NV+V GDFTHLSKQLRLLNWHSYPS+CLPL F+SRYLFQLLLPLS
Sbjct: 549 KVFEDMRSLKILEIGNVEVSGDFTHLSKQLRLLNWHSYPSQCLPLSFESRYLFQLLLPLS 608

Query: 481 QTRQLWNGQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLN 540
           QTRQLWNGQKGFEKLK+INVS SKNLRETPNFTKVPNLESL LSNCTRLWKIDSSISRLN
Sbjct: 609 QTRQLWNGQKGFEKLKVINVSGSKNLRETPNFTKVPNLESLDLSNCTRLWKIDSSISRLN 668

Query: 541 RLTLLDLTCCINLKSLPSIQSCKSLTTLNLAGSGLECLDQKGLEHF 585
           RLTLLD+TCCINLK+L   +SCKSL T+N  GSGLE   +KG  +F
Sbjct: 669 RLTLLDITCCINLKNLSFSRSCKSLITINYVGSGLE---EKGTCNF 710

BLAST of Bhi06M000158 vs. ExPASy TrEMBL
Match: M4QSV9 (Fom-1 OS=Cucumis melo OX=3656 GN=Fom-1 PE=4 SV=1)

HSP 1 Score: 912.9 bits (2358), Expect = 6.8e-262
Identity = 470/587 (80.07%), Postives = 519/587 (88.42%), Query Frame = 0

Query: 1   MERDKYMKEVQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLDILNHQMLVASDKV 60
           MERDKYM+EVQQWRRA TKVGDL+GVVVTKDSVEV SIGKIT QLLD+L    LV  D++
Sbjct: 129 MERDKYMEEVQQWRRAFTKVGDLTGVVVTKDSVEVDSIGKITNQLLDMLK---LVPWDEL 188

Query: 61  DRLVDMERRLSKMDELIDLESNDVRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFLR 120
            +LVD+ER+L KM++L DLE N VRF+GI GMGGIGKTTIAEVFYD+VA  FGKN CFLR
Sbjct: 189 TKLVDIERQLFKMEKLNDLEPNVVRFIGIIGMGGIGKTTIAEVFYDRVARTFGKNRCFLR 248

Query: 121 IYER-TLVSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDGVKEISQLEM 180
           IYE  TL+SLQQQLLSQLL+TKD++INNENEGARMI S LK K+V I LDGVKE +QLE 
Sbjct: 249 IYEHTTLLSLQQQLLSQLLQTKDLIINNENEGARMIGSRLKDKRVLIVLDGVKEKTQLEQ 308

Query: 181 LVGSPIWFGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAFGSEFP 240
           LVG+P WFG GSKIIITTRNRDVLRQPNY+D MVEY+VEFLD +SAM+LFCKHAFG  FP
Sbjct: 309 LVGNPNWFGSGSKIIITTRNRDVLRQPNYKDKMVEYSVEFLDTKSAMTLFCKHAFGCGFP 368

Query: 241 NRNFEDLSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMNKHLFKTLKIS 300
            +NF+D SKEIVERVKGHPQALIQIG SLYDKGI+IWKEELKSLE+DY N  +FKTLKIS
Sbjct: 369 RKNFKDFSKEIVERVKGHPQALIQIGSSLYDKGIEIWKEELKSLEEDY-NNRIFKTLKIS 428

Query: 301 FDDLGKTSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRRDNTIF 360
           FDDLGKTSQEVFLDLACFFN K KEKVIEILKSFDYRPHSE++LL+DRCLIEVR DNTI 
Sbjct: 429 FDDLGKTSQEVFLDLACFFNEKTKEKVIEILKSFDYRPHSEIQLLQDRCLIEVRSDNTIL 488

Query: 361 MPNCIQAMGQEIERGADKRSRIWLPKDAIDAFDQRH-RVRDIKGIVLEMEEKQDEVELEG 420
           MP CIQ MGQ+IER ADKRSRIWLPKDA D FD+ H RV+DIKG+VL++EEKQDE+ELEG
Sbjct: 489 MPKCIQTMGQQIEREADKRSRIWLPKDAQDVFDEPHQRVKDIKGVVLKLEEKQDEIELEG 548

Query: 421 KVFEDMTCLKILQISNVKVIGDFTHLSKQLRLLNWHSYPSECLPLRFDSRYLFQLLLPLS 480
           KVFEDM  LKIL+I NV+V GDFTHLSKQLRLLNWHSYPS+CLPL F+SRYLFQLLLPLS
Sbjct: 549 KVFEDMRSLKILEIGNVEVSGDFTHLSKQLRLLNWHSYPSQCLPLSFESRYLFQLLLPLS 608

Query: 481 QTRQLWNGQK-GFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRL 540
           QTRQLWNGQK GFEKLK+INVS SKNLRETPNFTKVPNLESL LSNCTRLWKIDSSISRL
Sbjct: 609 QTRQLWNGQKEGFEKLKVINVSGSKNLRETPNFTKVPNLESLDLSNCTRLWKIDSSISRL 668

Query: 541 NRLTLLDLTCCINLKSLPSIQSCKSLTTLNLAGSGLECLDQKGLEHF 585
           NRLTLLD+TCCINLK+LP  +SCKSL T+N  GSGLE   +KG  +F
Sbjct: 669 NRLTLLDITCCINLKNLPFSRSCKSLITINYVGSGLE---EKGTCNF 708

BLAST of Bhi06M000158 vs. ExPASy TrEMBL
Match: A0A6J1CK85 (TMV resistance protein N-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012052 PE=4 SV=1)

HSP 1 Score: 816.6 bits (2108), Expect = 6.7e-233
Identity = 414/578 (71.63%), Postives = 487/578 (84.26%), Query Frame = 0

Query: 9   EVQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLDILNHQMLVASDKVDRLVDMER 68
           EVQQWR A+TK+ +L+GVVVTK+SVEVASI KITKQ+ D+L++  LVA+DK++ LVD+ER
Sbjct: 145 EVQQWREAMTKIANLTGVVVTKESVEVASIDKITKQVFDMLHYPKLVAADKLNNLVDIER 204

Query: 69  RLSKMDELIDLESNDVRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFLRI--YERTL 128
           RL  MD LI LESNDV FVGI GMGGIGKTTIAEVFYDKVA+KFGKN CFLRI  +  +L
Sbjct: 205 RLYSMDTLIGLESNDVCFVGIIGMGGIGKTTIAEVFYDKVAHKFGKNRCFLRIQGHSLSL 264

Query: 129 VSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDGVKEISQLEMLVGSPIW 188
           +SLQ QLLSQLLR +DI I +ENEG  MI+ +L GKKV I LDG+ E  QLE L GSP W
Sbjct: 265 ISLQHQLLSQLLRKEDIKIMDENEGEYMIKYHLSGKKVLIVLDGIDEKGQLEKLAGSPDW 324

Query: 189 FGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAFGSE-FPNRNFED 248
           FG  S+IIITTRNRDVLRQ NY+D ++EY VE LDY+S  SLFCKHAFG    PN NFE 
Sbjct: 325 FGPKSQIIITTRNRDVLRQSNYKDKLLEYKVELLDYKSGFSLFCKHAFGDHTCPNENFEQ 384

Query: 249 LSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMNKHLFKTLKISFDDLGK 308
           LSKEIV+RVKGHPQAL+QIG  LY + ID+WKEEL SL+ DY NKH+FKTLKISFD L K
Sbjct: 385 LSKEIVDRVKGHPQALVQIGSYLYGQDIDVWKEELNSLDIDY-NKHVFKTLKISFDGLEK 444

Query: 309 TSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRRDNTIFMPNCIQ 368
           TSQ+VFLDLACFFNG++K+KVIEIL+SFDYRPHSE++LL DRCLIEVRRD TIF+PNCIQ
Sbjct: 445 TSQQVFLDLACFFNGRRKDKVIEILRSFDYRPHSEIQLLYDRCLIEVRRDGTIFIPNCIQ 504

Query: 369 AMGQEIERGADKRSRIWLPKDAIDAFDQRHRVRDIKGIVLEMEEKQDEVELEGKVFEDMT 428
            MG++IER AD+RSRIW+ KD +D FD+RH V+ I+GIVL++EEKQD+++L+  +FEDMT
Sbjct: 505 TMGRKIERDADERSRIWIRKDVLDVFDKRHGVKHIEGIVLDLEEKQDKLKLQAGLFEDMT 564

Query: 429 CLKILQISNVKVIGDFTHLSKQLRLLNWHSYPSECLPLRFDSRYLFQLLLPLSQTRQLWN 488
            L+IL+I NV+   DFTHLSKQLRLLNWH YPS CLPL+F+SRYLF+LLLP+ Q  QLW 
Sbjct: 565 GLRILEIGNVEFCEDFTHLSKQLRLLNWHGYPSTCLPLQFESRYLFELLLPVGQNTQLWK 624

Query: 489 GQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLLDL 548
           GQKGFEKLK+I+VSDS+NLRETPNFTKVPNLESLVL NCTRL+ ID SISRLNRLT LDL
Sbjct: 625 GQKGFEKLKVIDVSDSRNLRETPNFTKVPNLESLVLRNCTRLYMIDHSISRLNRLTSLDL 684

Query: 549 TCCINLKSLPSIQSCKSLTTLNLAGSGLECLDQKGLEH 584
           TCC++L+S   I SCKSLTTL LAGSGLE L  KG+++
Sbjct: 685 TCCVSLRSFSVINSCKSLTTLKLAGSGLERL--KGVDN 719

BLAST of Bhi06M000158 vs. ExPASy TrEMBL
Match: A0A6J1CJ23 (TMV resistance protein N-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012052 PE=4 SV=1)

HSP 1 Score: 815.5 bits (2105), Expect = 1.5e-232
Identity = 412/571 (72.15%), Postives = 482/571 (84.41%), Query Frame = 0

Query: 9   EVQQWRRALTKVGDLSGVVVTKDSVEVASIGKITKQLLDILNHQMLVASDKVDRLVDMER 68
           EVQQWR A+TK+ +L+GVVVTK+SVEVASI KITKQ+ D+L++  LVA+DK++ LVD+ER
Sbjct: 145 EVQQWREAMTKIANLTGVVVTKESVEVASIDKITKQVFDMLHYPKLVAADKLNNLVDIER 204

Query: 69  RLSKMDELIDLESNDVRFVGITGMGGIGKTTIAEVFYDKVANKFGKNHCFLRI--YERTL 128
           RL  MD LI LESNDV FVGI GMGGIGKTTIAEVFYDKVA+KFGKN CFLRI  +  +L
Sbjct: 205 RLYSMDTLIGLESNDVCFVGIIGMGGIGKTTIAEVFYDKVAHKFGKNRCFLRIQGHSLSL 264

Query: 129 VSLQQQLLSQLLRTKDIVINNENEGARMIESYLKGKKVFIALDGVKEISQLEMLVGSPIW 188
           +SLQ QLLSQLLR +DI I +ENEG  MI+ +L GKKV I LDG+ E  QLE L GSP W
Sbjct: 265 ISLQHQLLSQLLRKEDIKIMDENEGEYMIKYHLSGKKVLIVLDGIDEKGQLEKLAGSPDW 324

Query: 189 FGQGSKIIITTRNRDVLRQPNYRDIMVEYNVEFLDYESAMSLFCKHAFGSE-FPNRNFED 248
           FG  S+IIITTRNRDVLRQ NY+D ++EY VE LDY+S  SLFCKHAFG    PN NFE 
Sbjct: 325 FGPKSQIIITTRNRDVLRQSNYKDKLLEYKVELLDYKSGFSLFCKHAFGDHTCPNENFEQ 384

Query: 249 LSKEIVERVKGHPQALIQIGLSLYDKGIDIWKEELKSLEKDYMNKHLFKTLKISFDDLGK 308
           LSKEIV+RVKGHPQAL+QIG  LY + ID+WKEEL SL+ DY NKH+FKTLKISFD L K
Sbjct: 385 LSKEIVDRVKGHPQALVQIGSYLYGQDIDVWKEELNSLDIDY-NKHVFKTLKISFDGLEK 444

Query: 309 TSQEVFLDLACFFNGKKKEKVIEILKSFDYRPHSELKLLEDRCLIEVRRDNTIFMPNCIQ 368
           TSQ+VFLDLACFFNG++K+KVIEIL+SFDYRPHSE++LL DRCLIEVRRD TIF+PNCIQ
Sbjct: 445 TSQQVFLDLACFFNGRRKDKVIEILRSFDYRPHSEIQLLYDRCLIEVRRDGTIFIPNCIQ 504

Query: 369 AMGQEIERGADKRSRIWLPKDAIDAFDQRHRVRDIKGIVLEMEEKQDEVELEGKVFEDMT 428
            MG++IER AD+RSRIW+ KD +D FD+RH V+ I+GIVL++EEKQD+++L+  +FEDMT
Sbjct: 505 TMGRKIERDADERSRIWIRKDVLDVFDKRHGVKHIEGIVLDLEEKQDKLKLQAGLFEDMT 564

Query: 429 CLKILQISNVKVIGDFTHLSKQLRLLNWHSYPSECLPLRFDSRYLFQLLLPLSQTRQLWN 488
            L+IL+I NV+   DFTHLSKQLRLLNWH YPS CLPL+F+SRYLF+LLLP+ Q  QLW 
Sbjct: 565 GLRILEIGNVEFCEDFTHLSKQLRLLNWHGYPSTCLPLQFESRYLFELLLPVGQNTQLWK 624

Query: 489 GQKGFEKLKLINVSDSKNLRETPNFTKVPNLESLVLSNCTRLWKIDSSISRLNRLTLLDL 548
           GQKGFEKLK+I+VSDS+NLRETPNFTKVPNLESLVL NCTRL+ ID SISRLNRLT LDL
Sbjct: 625 GQKGFEKLKVIDVSDSRNLRETPNFTKVPNLESLVLRNCTRLYMIDHSISRLNRLTSLDL 684

Query: 549 TCCINLKSLPSIQSCKSLTTLNLAGSGLECL 577
           TCC++L+S   I SCKSLTTL LAGSGLE L
Sbjct: 685 TCCVSLRSFSVINSCKSLTTLKLAGSGLERL 714

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G72840.18.1e-7434.68Disease resistance protein (TIR-NBS-LRR class) [more]
AT1G72840.28.1e-7434.68Disease resistance protein (TIR-NBS-LRR class) [more]
AT1G63870.12.6e-7231.14Disease resistance protein (TIR-NBS-LRR class) family [more]
AT4G19510.21.7e-7132.71Disease resistance protein (TIR-NBS-LRR class) [more]
AT5G17970.14.9e-7129.78Disease resistance protein (TIR-NBS-LRR class) family [more]
Match NameE-valueIdentityDescription
V9M2S52.3e-8135.17Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=... [more]
V9M3987.7e-7734.75Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=... [more]
A0A290U7C43.9e-7332.93Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV... [more]
Q403927.4e-7232.57TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1[more]
F4JT802.4e-7032.71Disease resistance protein RPP2B OS=Arabidopsis thaliana OX=3702 GN=RPP2B PE=1 S... [more]
Match NameE-valueIdentityDescription
A0A1S3C9Z09.8e-26980.78TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103498649 PE=4 SV=1[more]
Q5DMW27.8e-26680.38MRGH9 OS=Cucumis melo OX=3656 GN=MRGH9 PE=4 SV=1[more]
M4QSV96.8e-26280.07Fom-1 OS=Cucumis melo OX=3656 GN=Fom-1 PE=4 SV=1[more]
A0A6J1CK856.7e-23371.63TMV resistance protein N-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1CJ231.5e-23272.15TMV resistance protein N-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC11... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 86..101
score: 63.28
coord: 511..527
score: 32.65
coord: 254..268
score: 36.5
coord: 158..172
score: 41.5
NoneNo IPR availablePANTHERPTHR11017:SF406TMV RESISTANCE PROTEIN N-LIKEcoord: 33..570
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 421..587
IPR035897Toll/interleukin-1 receptor homology (TIR) domain superfamilyGENE3D3.40.50.10140coord: 1..53
e-value: 2.6E-5
score: 25.9
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 54..215
e-value: 4.6E-29
score: 103.2
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 60..314
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 80..301
e-value: 2.5E-27
score: 95.7
IPR042197Apoptotic protease-activating factors, helical domainGENE3D1.10.8.430coord: 220..304
e-value: 2.0E-10
score: 42.1
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 395..590
e-value: 4.1E-17
score: 63.3
IPR044974Disease resistance protein, plantsPANTHERPTHR11017LEUCINE-RICH REPEAT-CONTAINING PROTEINcoord: 33..570
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 293..390

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Bhi06G000158Bhi06G000158gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi06M000158.exon1Bhi06M000158.exon1exon
Bhi06M000158.exon2Bhi06M000158.exon2exon
Bhi06M000158.exon3Bhi06M000158.exon3exon
Bhi06M000158.exon4Bhi06M000158.exon4exon
Bhi06M000158.exon5Bhi06M000158.exon5exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Bhi06M000158cds.Bhi06M000158CDS
cds.Bhi06M000158cds.Bhi06M000158_2CDS
cds.Bhi06M000158cds.Bhi06M000158_3CDS
cds.Bhi06M000158cds.Bhi06M000158_4CDS
cds.Bhi06M000158cds.Bhi06M000158_5CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Bhi06M000158Bhi06M000158-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0007165 signal transduction
molecular_function GO:0043531 ADP binding
molecular_function GO:0003953 NAD+ nucleosidase activity