Bhi06M000094 (mRNA) Wax gourd (B227) v1

Overview
NameBhi06M000094
TypemRNA
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionCytochrome P450, putative
Locationchr6: 2243697 .. 2247829 (+)
Sequence length2036
RNA-Seq ExpressionBhi06M000094
SyntenyBhi06M000094
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCTTTTCCCCAACTCTGAATCTCTCTTCTGTGTTTGGCAAAATTTAAGTTTTATATATATAAATAAATTCAAAACACTTATTATACCCTCTCTGATCTCTCAATGCCCATCATGTTGCCACTCCTCCTTACAACTCTAGTTCTCCTAACGACGTCGTTTTTCATCTTCTCTTTACTATACGACACCCTTTCTTGCTACTGCTTCACTCCTAGACGCATCAAGCACAAGATGGCAAAGCAAGGCGTGCGCGGCCCTAAACCCCGCCCTCTCCTCGGCAACATCCTCGACGTCGCCGCCCTCCTCTCCAAATCCACCTCCTCCGACATGCCTTCCATCTCTCACGACCTCGTCCCCCGTCTCCTCCCTCATTTCCTCGCCTGGTCCACCCAGTTTGGTACGTCGATGATGAGTCAATCATGATGATGACCCTCAAATTATTTGCATTGCGACGATTATCATCGTTAAGTAATTTATTTTATTTCATCTTTTCAGGGAAGAGATTTATATTTTGGAATGGGATTGAGCCAAGAATGTGTCTGACAGAAACGGATTTGATAAAAGAGCTTTTGTCTAAATACAGCGCCGTTTCGGGCAAGTCATGGCTTCAACAACAAGGCTCTAAGCACTTTATTGGCCGTGGTCTTTTAATGGCCAACGGCCAAAATTGGTTCCATCAACGCCATATTGTCGCACCCGCTTTTGTCGGTGACCGACTTAAGGTCCGTACGCTTTTGTGGTTTTTTTTTTTTTTTTAAAAAAAAAAAATTATAATATGTGAGTGGGGAAATCCAACTTCTAGCGTAAATGATTTAAGAACACGAATAAATGATTATTTATTATTATTTTTATTTTTGGATTTGAATTTTAGTGTTTTGTTTTGTTTTTTCAGAGTTATGCGGGGTATATGGTGGAGTGTACAAGGGAGATGCTGGAGTCAATAGAAAATGAGGTGAAATCGGGGCGATCAGAATTTGAAATCGGCGAGTATATGACAAGATTAACGGCGGATATAATATCGAGAACGGAGTTTGAAAGCAGTTACGAAAAGGGAAAACAAATCTTTCATTTACTTACTGTTTTACAGCATCTCTGTGCCCAGGCCAGCCGCCACCTCTGTCTTCCCGGTAGCCGGTAACAATTTTTTTCTTTTCTCCTTTTTTTTTTTTTTAATGCTGACAGTGCTAACCTTTGACATGGCTTTATTTTCATTTTTTTTTTTAAAAAAAAAATCTAATTTCTCTCTTCTCTCTCCTCTTTTTTCACTCTCTTTTCACTCTCTTTTTTTAGCTACATTTTGTTAATTTTTTTTTTTTACCATCGTGGAGTGTAGAATTGAACTTATAATTTTAAGATTGATAATGCAAACATTATGTGAGTGTCCTATGCTCATTTTGATCTCTTTTCTCTTTTATTGTTGGAACAAAAATATTAGCTCATGATAAAAAAAAACTTGGGTGTGCAATGTTGTAGTTTTAAGGACATTTTCGTCTTTTTTGTATTATCACGAGTGCAATTGCTTCGTTTGATCTCTTAATGCATCGTTTTAGATCAAGTCACACTTACAACATTTCAACGACCTCTTAAGCTCGATTTCTTGTAACATTAATTTTCTAACTATTAACTCGATTTTGGTCGGAAAAAACAACTATTATTAAGAGCTATCAAATCATCATAAATTACATTTTTTCAATCAAACAAGGTTATTAGGTACAATTTGTCATGTGACAAATTTTTTTCTTTTTAAAATTTTTTTAAGTTTTTATTCTTCCGTATAATTCTAGTAGATATATACTATAGAAAGTGTGCAAAAAAAAAAAAAAAAAAAAAGAATTCAAACACATAAAAGTGAATGTTATTTTAGAGTAATATTTTGATGATGGGTAGTTTTTAATTTGGGTTTTTGGCAGGTTTTTTCCAAGTAAATATAACAGAGAAATAAAGGCATTGAAAGGGAAAGTGGAGGAATTGTTAATGGAGATAATACAAAGCAGAAGGGATTGTGTGGAAATTGGGAGAAGCAGTTCATATGGGAATGATTTATTGGGAATGTTGCTAAATGAAATGCAAAAGAAAAAAGATGGGAATGGTTTGAGTTTGAATTTGCAAATTATAATGGATGAATGCAAAACATTCTTCTTTGCTGGCCATGAAACCACTGCCCTTTTGCTCACTTGGACTGTCATGTTATTAGCTACTAACCCTTCTTGGCAACACAAGCTTCGAGCCGAAGTTATCGCTATCTGTGGTTCTGAAACGCCCTCGTTTCATCATCTTTCCAAACTTTCTCTGGTAAGAAATGTCGAGATTTTAATTTTATAAATTATTTTTTAAAAAATTTAATGTGATAATCAAGTTTTTCACCCTTTTAAAGCAAGTTAAAATTTCTTATTATTTGTATTTTATATAACATTTTTCTATTACTATAATATAAAGCCCAAAAAAATTATTAATAAGAAGATGAAAATTTAATATCCTAAAAAAGTGGGGGATGGATAACCCACTAAGGTTCCCAAACTGTCTTTTCAGCTATAATAGAAGATTTTTCAAAAGAATTTTATAATTTGGAAAAGGAAGCCTGAGTTTTGCAATCATTTTATTTTTTTTATTACCATTTTCTATTTTTTCTAAGTAAAAGAATTGAATTTTTATTTAAATGTTAAAAATAATAATAATTTTTTTTAAAAAAAAAAATAGTTTGATTTTTTAAAACATAAGGTAGATAACCAAATAAGATAAAAATTTATGGATTTAATTTTGAACAAATAAAAATTATAAACTAAATAGATAAATTAATTTATGGAAGGAAAAAAAAGATTGTGGGTGGGACCCTAGTTCATTTTAATATGGCTTACTTCATGGCACACCACTTATAAATGTCTTTTTCCTCAACTGAATTTTCACAAATTTTAGAAGAAAATTAATTAATTAATTAATTAAAATCCAAATATTCCACTTAACCAAAACTAATGATTGTTGTAGCCACTTCAGTTGGCCCTGAAAAGACATTAAAACTCATAAACACATGGTCAATCTTTTACCATTTACAATTTTTTTTTTATGGATTTAAATGAGCTTAATTAACATATTTTTATTACAATATAAGGAAATACTTGTACATTTACTTATTTACCCTTACATTTGTTTGATAAATAAATATTCATATATAGACATGATTAGGTACAACCTTTTACAAAATAAAAATAATTGAATATTTTAAAAGTATTGATCACATGACGGATTTTTATCGAGTGTTTTCTACGTAACTGCATCCAAATTTTTTCCGATTATAATTATTTATATCTTTTTATTCGTGTACATTTACTTATTTGTCCTTAATTACAGTTAGGTATGGTGATAAATGAATCGCTAAGACTATACCCACCAGCAAGTATTCTTCCAAGAATGGCATTTGAAGATATAAAACTTGGAGATTTAGAGATCCCAAAAGGATTATCAATATGGATTCCAGTGTTAGCCATTCATCATAGTGAAGAAATATGGGGAAAAGATGCAAATGAATTCAATCCAGAAAGATTTGCCAATTCAAAAGCTTTTACTTCTGGTGGTTTCATCCCTTTTGCTTCTGGCCCTCGCAATTGTGTTGGCCAATCTTTTGCTCTTATGGAAACTAAGATCATCTTGGCTATGCTCATCTCCAAATTTAGCTTCACGATCTCCGATAAATACCGACATGCACCCGTTGTCGTCCTCACTATAAAACCTAAATACGGCGTTCAAGTTTGTTTGAAGCCTTTGAATACACAGAGTTAATACTTGAATGCATCGTGAGTTGCACCCATCTTTTTGTACCTCGTTCCAACCAGACACTAGAAAGAATAATTTAAGGAAAAAAAAAAAAAGAATTGATTGGACAGCTGAAATAGATGGGTGTAGCGTAGAATGCACTCAAGTACTTTTCCTTAATAAATTTTCTCTAAGGTAGAGTTTCTTTTTCCTTTTTGAGACGAGAGAGAGATTTGGTCATTTTTCACTCGTTTGTTTTTGTTTTTCTTTTTGCTCTCTCTTTCTGTTTTTGTCATTGTGAAGAGAAAGAGAGATGTGGGAGAATATGAAGTTTGGTTGTAATGTTATATTTTGAGCAATATTAAATAGAGGCAACCGTTTTTTTTTC

mRNA sequence

CCCTTTTCCCCAACTCTGAATCTCTCTTCTGTGTTTGGCAAAATTTAAGTTTTATATATATAAATAAATTCAAAACACTTATTATACCCTCTCTGATCTCTCAATGCCCATCATGTTGCCACTCCTCCTTACAACTCTAGTTCTCCTAACGACGTCGTTTTTCATCTTCTCTTTACTATACGACACCCTTTCTTGCTACTGCTTCACTCCTAGACGCATCAAGCACAAGATGGCAAAGCAAGGCGTGCGCGGCCCTAAACCCCGCCCTCTCCTCGGCAACATCCTCGACGTCGCCGCCCTCCTCTCCAAATCCACCTCCTCCGACATGCCTTCCATCTCTCACGACCTCGTCCCCCGTCTCCTCCCTCATTTCCTCGCCTGGTCCACCCAGTTTGGGAAGAGATTTATATTTTGGAATGGGATTGAGCCAAGAATGTGTCTGACAGAAACGGATTTGATAAAAGAGCTTTTGTCTAAATACAGCGCCGTTTCGGGCAAGTCATGGCTTCAACAACAAGGCTCTAAGCACTTTATTGGCCGTGGTCTTTTAATGGCCAACGGCCAAAATTGGTTCCATCAACGCCATATTGTCGCACCCGCTTTTGTCGGTGACCGACTTAAGAGTTATGCGGGGTATATGGTGGAGTGTACAAGGGAGATGCTGGAGTCAATAGAAAATGAGGTGAAATCGGGGCGATCAGAATTTGAAATCGGCGAGTATATGACAAGATTAACGGCGGATATAATATCGAGAACGGAGTTTGAAAGCAGTTACGAAAAGGGAAAACAAATCTTTCATTTACTTACTGTTTTACAGCATCTCTGTGCCCAGGCCAGCCGCCACCTCTGTCTTCCCGGTAGCCGGTTTTTTCCAAGTAAATATAACAGAGAAATAAAGGCATTGAAAGGGAAAGTGGAGGAATTGTTAATGGAGATAATACAAAGCAGAAGGGATTGTGTGGAAATTGGGAGAAGCAGTTCATATGGGAATGATTTATTGGGAATGTTGCTAAATGAAATGCAAAAGAAAAAAGATGGGAATGGTTTGAGTTTGAATTTGCAAATTATAATGGATGAATGCAAAACATTCTTCTTTGCTGGCCATGAAACCACTGCCCTTTTGCTCACTTGGACTGTCATGTTATTAGCTACTAACCCTTCTTGGCAACACAAGCTTCGAGCCGAAGTTATCGCTATCTGTGGTTCTGAAACGCCCTCGTTTCATCATCTTTCCAAACTTTCTCTGTTAGGTATGGTGATAAATGAATCGCTAAGACTATACCCACCAGCAAGTATTCTTCCAAGAATGGCATTTGAAGATATAAAACTTGGAGATTTAGAGATCCCAAAAGGATTATCAATATGGATTCCAGTGTTAGCCATTCATCATAGTGAAGAAATATGGGGAAAAGATGCAAATGAATTCAATCCAGAAAGATTTGCCAATTCAAAAGCTTTTACTTCTGGTGGTTTCATCCCTTTTGCTTCTGGCCCTCGCAATTGTGTTGGCCAATCTTTTGCTCTTATGGAAACTAAGATCATCTTGGCTATGCTCATCTCCAAATTTAGCTTCACGATCTCCGATAAATACCGACATGCACCCGTTGTCGTCCTCACTATAAAACCTAAATACGGCGTTCAAGTTTGTTTGAAGCCTTTGAATACACAGAGTTAATACTTGAATGCATCGTGAGTTGCACCCATCTTTTTGTACCTCGTTCCAACCAGACACTAGAAAGAATAATTTAAGGAAAAAAAAAAAAAGAATTGATTGGACAGCTGAAATAGATGGGTGTAGCGTAGAATGCACTCAAGTACTTTTCCTTAATAAATTTTCTCTAAGGTAGAGTTTCTTTTTCCTTTTTGAGACGAGAGAGAGATTTGGTCATTTTTCACTCGTTTGTTTTTGTTTTTCTTTTTGCTCTCTCTTTCTGTTTTTGTCATTGTGAAGAGAAAGAGAGATGTGGGAGAATATGAAGTTTGGTTGTAATGTTATATTTTGAGCAATATTAAATAGAGGCAACCGTTTTTTTTTC

Coding sequence (CDS)

ATGCCCATCATGTTGCCACTCCTCCTTACAACTCTAGTTCTCCTAACGACGTCGTTTTTCATCTTCTCTTTACTATACGACACCCTTTCTTGCTACTGCTTCACTCCTAGACGCATCAAGCACAAGATGGCAAAGCAAGGCGTGCGCGGCCCTAAACCCCGCCCTCTCCTCGGCAACATCCTCGACGTCGCCGCCCTCCTCTCCAAATCCACCTCCTCCGACATGCCTTCCATCTCTCACGACCTCGTCCCCCGTCTCCTCCCTCATTTCCTCGCCTGGTCCACCCAGTTTGGGAAGAGATTTATATTTTGGAATGGGATTGAGCCAAGAATGTGTCTGACAGAAACGGATTTGATAAAAGAGCTTTTGTCTAAATACAGCGCCGTTTCGGGCAAGTCATGGCTTCAACAACAAGGCTCTAAGCACTTTATTGGCCGTGGTCTTTTAATGGCCAACGGCCAAAATTGGTTCCATCAACGCCATATTGTCGCACCCGCTTTTGTCGGTGACCGACTTAAGAGTTATGCGGGGTATATGGTGGAGTGTACAAGGGAGATGCTGGAGTCAATAGAAAATGAGGTGAAATCGGGGCGATCAGAATTTGAAATCGGCGAGTATATGACAAGATTAACGGCGGATATAATATCGAGAACGGAGTTTGAAAGCAGTTACGAAAAGGGAAAACAAATCTTTCATTTACTTACTGTTTTACAGCATCTCTGTGCCCAGGCCAGCCGCCACCTCTGTCTTCCCGGTAGCCGGTTTTTTCCAAGTAAATATAACAGAGAAATAAAGGCATTGAAAGGGAAAGTGGAGGAATTGTTAATGGAGATAATACAAAGCAGAAGGGATTGTGTGGAAATTGGGAGAAGCAGTTCATATGGGAATGATTTATTGGGAATGTTGCTAAATGAAATGCAAAAGAAAAAAGATGGGAATGGTTTGAGTTTGAATTTGCAAATTATAATGGATGAATGCAAAACATTCTTCTTTGCTGGCCATGAAACCACTGCCCTTTTGCTCACTTGGACTGTCATGTTATTAGCTACTAACCCTTCTTGGCAACACAAGCTTCGAGCCGAAGTTATCGCTATCTGTGGTTCTGAAACGCCCTCGTTTCATCATCTTTCCAAACTTTCTCTGTTAGGTATGGTGATAAATGAATCGCTAAGACTATACCCACCAGCAAGTATTCTTCCAAGAATGGCATTTGAAGATATAAAACTTGGAGATTTAGAGATCCCAAAAGGATTATCAATATGGATTCCAGTGTTAGCCATTCATCATAGTGAAGAAATATGGGGAAAAGATGCAAATGAATTCAATCCAGAAAGATTTGCCAATTCAAAAGCTTTTACTTCTGGTGGTTTCATCCCTTTTGCTTCTGGCCCTCGCAATTGTGTTGGCCAATCTTTTGCTCTTATGGAAACTAAGATCATCTTGGCTATGCTCATCTCCAAATTTAGCTTCACGATCTCCGATAAATACCGACATGCACCCGTTGTCGTCCTCACTATAAAACCTAAATACGGCGTTCAAGTTTGTTTGAAGCCTTTGAATACACAGAGTTAA

Protein sequence

MPIMLPLLLTTLVLLTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNILDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTREMLESIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLGMLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKLRAEVIAICGSETPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEEIWGKDANEFNPERFANSKAFTSGGFIPFASGPRNCVGQSFALMETKIILAMLISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPLNTQS
Homology
BLAST of Bhi06M000094 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 731.9 bits (1888), Expect = 3.7e-211
Identity = 359/518 (69.31%), Postives = 428/518 (82.63%), Query Frame = 0

Query: 3   IMLPLLLTTLVLLTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNILD 62
           ++L +L + LV+  T+  I  +LYDT+SCY  TPRRIK  M +QGV GPKPRPL GNIL+
Sbjct: 1   MLLTILKSLLVIFVTT--ILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILE 60

Query: 63  VAALLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIKEL 122
           ++A++S+S S D  SI HD+V RLLPH++AWS Q+GKRFI WNG +PR+CLTET+LIKEL
Sbjct: 61  ISAMVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKEL 120

Query: 123 LSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVEC 182
           L K++ VSG+SWLQQQG+K+FIGRGLLMANGQ+W HQRH+ APAF G+RLK YA +MVEC
Sbjct: 121 LMKHNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVEC 180

Query: 183 TREMLESIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCA 242
           T +++E +  EV  G +E EIGE M +LTADIISRT+F SS+EKGK++F+ LTVLQ  CA
Sbjct: 181 TSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCA 240

Query: 243 QASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLGML 302
           QA+RHLC PGSRF PSKYNREIK+LK +VE LL+EIIQSRRDC E+GRSS++G+DLLG+L
Sbjct: 241 QATRHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLL 300

Query: 303 LNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKLRAEV 362
           LNEM   K+ N  + NLQ+IMDECKTFFFAGHETTALLLTWT MLLA NP+WQ K+R EV
Sbjct: 301 LNEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEV 360

Query: 363 IAICG-SETPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW 422
             + G +  PS   LSKL+ L  VINESLRLYPPA++LPRMAFED+KLGDL IPKGLSIW
Sbjct: 361 REVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIW 420

Query: 423 IPVLAIHHSEEIWGKDANEFNPERFANSKAFTSGGFIPFASGPRNCVGQSFALMETKIIL 482
           IPVLAIHHSEE+WGKDAN+FNPERF      +   FIPFA+GPRNC+GQ FALME KIIL
Sbjct: 421 IPVLAIHHSEELWGKDANQFNPERFGGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIIL 480

Query: 483 AMLISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPL 520
           A LISKF+FTIS  YRHAP+VVLTIKPKYGVQV LKPL
Sbjct: 481 ATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of Bhi06M000094 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 710.3 bits (1832), Expect = 1.1e-204
Identity = 351/518 (67.76%), Postives = 418/518 (80.69%), Query Frame = 0

Query: 4   MLPLLLTTLVLLTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNILDV 63
           M+  L+   VL+     I  +LYD++ CY  TPRRIK  M +QG+ GPKPR L GNI+D+
Sbjct: 1   MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60

Query: 64  AALLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIKELL 123
           + +LS S S+D  SI H++VPRLLPH+++WS Q+GKRFI WNG EPR+CLTET++IKELL
Sbjct: 61  SKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELL 120

Query: 124 SKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECT 183
           +K++ V+GKSWLQQQG+K FIGRGLLMANG+ W HQRH+ APAF  DRLK YA +MVECT
Sbjct: 121 TKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECT 180

Query: 184 REMLESIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 243
           + M E +  EV     E EIGE M RLTADIISRTEF SS +KGK++F LLTVLQ LCAQ
Sbjct: 181 KMMAERLRKEV---GEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQ 240

Query: 244 ASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLGMLL 303
           A+RHLC PGSRF PSKYNREIK+LK +VE LLMEII SR+D VEIGRSSSYG+DLLG+LL
Sbjct: 241 ATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLL 300

Query: 304 NEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKLRAEVI 363
           N+M    D N  +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP+WQ  +R EV 
Sbjct: 301 NQM----DSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVR 360

Query: 364 AICGSE-TPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWI 423
            +CG +  PS   LS L+ L  VINESLRLYPPA++LPRMAFEDIKLGDL IPKGLSIWI
Sbjct: 361 QVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWI 420

Query: 424 PVLAIHHSEEIWGKDANEFNPERFANSKAFTSGGFIPFASGPRNCVGQSFALMETKIILA 483
           PVLAIHHS E+WG+DANEFNPERF      +S  F+PFA+GPRNC+GQ+FA+ME KIILA
Sbjct: 421 PVLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILA 480

Query: 484 MLISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPLN 521
           ML+SKFSF IS+ YRHAP+VVLTIKPKYGVQ+ LKPL+
Sbjct: 481 MLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of Bhi06M000094 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 367.1 bits (941), Expect = 2.4e-101
Identity = 206/526 (39.16%), Postives = 302/526 (57.41%), Query Frame = 0

Query: 8   LLTTLVLLTTSFFIFSL--LYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNILDVAA 67
           LL+T+ LL  +  +  +  +Y       + P  +  +  KQG+ GPK R L GN+ ++  
Sbjct: 58  LLSTINLLAIALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREIRK 117

Query: 68  LLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIKELLSK 127
           + +++    +   S+D+VPR+LPH   W +Q+G+ F++W G +PR+C+++ +L K++LS 
Sbjct: 118 MKNEAKLMVLDPNSNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQILSN 177

Query: 128 YSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTRE 187
                 KS  + +  K   G GL+  NG +W   R I+ PAF  D+LK     MV+CT  
Sbjct: 178 KFVFFSKSKTKPEILK-LSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFR 237

Query: 188 MLESIENEVKSGRSE--FEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 247
           M    + +     +E    I     RLTADII+   F SSY +G ++F     LQ  CA 
Sbjct: 238 MFLEWKKQRNGVETEQFVLISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAA 297

Query: 248 ASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLGMLL 307
           A   L  PG ++ P+  N +I  L  KV   +  II +R        S  YGNDLLG++L
Sbjct: 298 ALTDLYFPGIQYLPTPSNLQIWKLDMKVNSSIKRIIDARL----TSESKDYGNDLLGIML 357

Query: 308 -----NEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKL 367
                NE +KK       +++  I++ECKTFFFAGHETTA LLTW+ MLL+ +  WQ KL
Sbjct: 358 TAASSNESEKK-------MSIDEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKL 417

Query: 368 RAEVIAICGSE-TPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 427
           R EV   CG +  P     SKL L+  V  ESLRLY P   L R+A ED+KLG+LEIPKG
Sbjct: 418 REEVFNECGKDKIPDAETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKG 477

Query: 428 LSIWIPVLAIHHSEEIWGKDANEFNPERFANS---KAFTSGGFIPFASGPRNCVGQSFAL 487
            +I +P+  +H  + +WG DA++FNP RFAN     A      + F+ GPR C+GQ+FA+
Sbjct: 478 TTIILPIAKMHRDKAVWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAI 537

Query: 488 METKIILAMLISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPLN 521
           ME K +LAM++ +F   +S  Y+HAP   LT++P+Y + V L+P++
Sbjct: 538 MEAKTVLAMILQRFRLNLSADYKHAPADHLTLQPQYDLPVILEPID 571

BLAST of Bhi06M000094 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 366.7 bits (940), Expect = 3.1e-101
Identity = 203/527 (38.52%), Postives = 308/527 (58.44%), Query Frame = 0

Query: 3   IMLPLLLTTLVLLTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNILD 62
           I L L++  L++    F  F +L        + P  +  ++  QG+ GP  R   GN+ +
Sbjct: 5   IFLALIVLILIIGLRIFKAFMIL-------VWHPFVLTRRLKNQGISGPNYRIFYGNLSE 64

Query: 63  VAALLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIKEL 122
           +  +  +S  S +   S+D+ PR+LPH+  W +Q+G+ F++WNG EPR+C+++ +L K +
Sbjct: 65  IKKMKRESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTM 124

Query: 123 LSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVEC 182
           LS       KS  + +  K    +GL+   G +W   R I+ PAF  DRLK     MV+C
Sbjct: 125 LSNKLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVDC 184

Query: 183 TREML-----ESIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVL 242
           T +ML     ES + E +  + + E+ E   RLTADII+ + F SSY +G ++F     L
Sbjct: 185 TLKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMEL 244

Query: 243 QHLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGND 302
           +     +   + +PG+++ P+  N  +  L+ K++  +  II SR     +   S YG+D
Sbjct: 245 KRCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSR-----LQSKSDYGDD 304

Query: 303 LLGMLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHK 362
           LLG+LL       +G    ++++ I+ EC+TFFF GHETT+ LL WT MLL+ +  WQ K
Sbjct: 305 LLGILLKAY--NTEGKERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEK 364

Query: 363 LRAEVIAICGSE-TPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPK 422
           LR E+   CG E TP     SKL L+ MVI ESLRLY P S L R A  +IKLGDLEIPK
Sbjct: 365 LREEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIPK 424

Query: 423 GLSIWIPVLAIHHSEEIWGKDANEFNPERFANS---KAFTSGGFIPFASGPRNCVGQSFA 482
           G ++ IP+L +H  + +WG DA++FNP RFAN     A      + F+ GPR C+GQ+F 
Sbjct: 425 GTTVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQNFV 484

Query: 483 LMETKIILAMLISKFSF-TISDKYRHAPVVVLTIKPKYGVQVCLKPL 520
           ++E K +L M++ +F F ++ D+Y+H PV  +TI+P+YG+ V L+PL
Sbjct: 485 MIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 517

BLAST of Bhi06M000094 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 350.1 bits (897), Expect = 3.0e-96
Identity = 185/515 (35.92%), Postives = 299/515 (58.06%), Query Frame = 0

Query: 14  LLTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNILDVAALLSKSTSS 73
           +L  S  +  ++   +S   + PR+I+   +KQG+RGP     +GN+ ++  ++ K++S 
Sbjct: 13  VLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASSH 72

Query: 74  DMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVSGKS 133
            MP  SH+++PR+L  +  W   +G  F+ W G   R+ + + DLI+E+ SK        
Sbjct: 73  PMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIFSKSEFYEKNE 132

Query: 134 WLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTREMLESIENE 193
                  K   G GLL   G+ W H R I++P F  + LK     +++   +M++   ++
Sbjct: 133 --AHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDK 192

Query: 194 V-KSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHLCLPG 253
           + ++G  E ++ E+   LT D+ISRT F SSYE G+ +F L      LCA+A + + +PG
Sbjct: 193 LSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIPG 252

Query: 254 SRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSS----YGNDLLGMLLNEMQK 313
            RFFP++ N +   L  ++ + L+++I+ RR     G           DLLG+++     
Sbjct: 253 YRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMI----- 312

Query: 314 KKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKLRAEVIAICGS 373
                  ++ +Q I++ECK+FFFAG +TT+ LLTWT +LL+ +P WQ K R EV+ +CGS
Sbjct: 313 ----QAKNVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGS 372

Query: 374 -ETPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAI 433
            + P+  H+ KL  L M++NESLRLYPP     R A  D+KLG  +IP G  + IP++A+
Sbjct: 373 RDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAV 432

Query: 434 HHSEEIWGKDANEFNPERFANS---KAFTSGGFIPFASGPRNCVGQSFALMETKIILAML 493
           HH + IWG D NEFNP RFA+     A    GFIPF  G R C+GQ+ A+++ K+ LA++
Sbjct: 433 HHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVM 492

Query: 494 ISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPL 520
           I +F+F ++  Y+HAP V++ + P++G  +  + L
Sbjct: 493 IQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRL 515

BLAST of Bhi06M000094 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 731.9 bits (1888), Expect = 5.2e-210
Identity = 359/518 (69.31%), Postives = 428/518 (82.63%), Query Frame = 0

Query: 3   IMLPLLLTTLVLLTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNILD 62
           ++L +L + LV+  T+  I  +LYDT+SCY  TPRRIK  M +QGV GPKPRPL GNIL+
Sbjct: 1   MLLTILKSLLVIFVTT--ILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILE 60

Query: 63  VAALLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIKEL 122
           ++A++S+S S D  SI HD+V RLLPH++AWS Q+GKRFI WNG +PR+CLTET+LIKEL
Sbjct: 61  ISAMVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKEL 120

Query: 123 LSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVEC 182
           L K++ VSG+SWLQQQG+K+FIGRGLLMANGQ+W HQRH+ APAF G+RLK YA +MVEC
Sbjct: 121 LMKHNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVEC 180

Query: 183 TREMLESIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCA 242
           T +++E +  EV  G +E EIGE M +LTADIISRT+F SS+EKGK++F+ LTVLQ  CA
Sbjct: 181 TSKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCA 240

Query: 243 QASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLGML 302
           QA+RHLC PGSRF PSKYNREIK+LK +VE LL+EIIQSRRDC E+GRSS++G+DLLG+L
Sbjct: 241 QATRHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLL 300

Query: 303 LNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKLRAEV 362
           LNEM   K+ N  + NLQ+IMDECKTFFFAGHETTALLLTWT MLLA NP+WQ K+R EV
Sbjct: 301 LNEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEV 360

Query: 363 IAICG-SETPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW 422
             + G +  PS   LSKL+ L  VINESLRLYPPA++LPRMAFED+KLGDL IPKGLSIW
Sbjct: 361 REVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIW 420

Query: 423 IPVLAIHHSEEIWGKDANEFNPERFANSKAFTSGGFIPFASGPRNCVGQSFALMETKIIL 482
           IPVLAIHHSEE+WGKDAN+FNPERF      +   FIPFA+GPRNC+GQ FALME KIIL
Sbjct: 421 IPVLAIHHSEELWGKDANQFNPERFGGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIIL 480

Query: 483 AMLISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPL 520
           A LISKF+FTIS  YRHAP+VVLTIKPKYGVQV LKPL
Sbjct: 481 ATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKPL 516

BLAST of Bhi06M000094 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 1.6e-203
Identity = 351/518 (67.76%), Postives = 418/518 (80.69%), Query Frame = 0

Query: 4   MLPLLLTTLVLLTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNILDV 63
           M+  L+   VL+     I  +LYD++ CY  TPRRIK  M +QG+ GPKPR L GNI+D+
Sbjct: 1   MMVTLVLKYVLVIVMTLILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDI 60

Query: 64  AALLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIKELL 123
           + +LS S S+D  SI H++VPRLLPH+++WS Q+GKRFI WNG EPR+CLTET++IKELL
Sbjct: 61  SKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELL 120

Query: 124 SKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECT 183
           +K++ V+GKSWLQQQG+K FIGRGLLMANG+ W HQRH+ APAF  DRLK YA +MVECT
Sbjct: 121 TKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVECT 180

Query: 184 REMLESIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 243
           + M E +  EV     E EIGE M RLTADIISRTEF SS +KGK++F LLTVLQ LCAQ
Sbjct: 181 KMMAERLRKEV---GEEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLCAQ 240

Query: 244 ASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLGMLL 303
           A+RHLC PGSRF PSKYNREIK+LK +VE LLMEII SR+D VEIGRSSSYG+DLLG+LL
Sbjct: 241 ATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGLLL 300

Query: 304 NEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKLRAEVI 363
           N+M    D N  +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP+WQ  +R EV 
Sbjct: 301 NQM----DSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDEVR 360

Query: 364 AICGSE-TPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWI 423
            +CG +  PS   LS L+ L  VINESLRLYPPA++LPRMAFEDIKLGDL IPKGLSIWI
Sbjct: 361 QVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSIWI 420

Query: 424 PVLAIHHSEEIWGKDANEFNPERFANSKAFTSGGFIPFASGPRNCVGQSFALMETKIILA 483
           PVLAIHHS E+WG+DANEFNPERF      +S  F+PFA+GPRNC+GQ+FA+ME KIILA
Sbjct: 421 PVLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKIILA 480

Query: 484 MLISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPLN 521
           ML+SKFSF IS+ YRHAP+VVLTIKPKYGVQ+ LKPL+
Sbjct: 481 MLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of Bhi06M000094 vs. ExPASy Swiss-Prot
Match: F4IK45 (Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 3.4e-100
Identity = 206/526 (39.16%), Postives = 302/526 (57.41%), Query Frame = 0

Query: 8   LLTTLVLLTTSFFIFSL--LYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNILDVAA 67
           LL+T+ LL  +  +  +  +Y       + P  +  +  KQG+ GPK R L GN+ ++  
Sbjct: 58  LLSTINLLAIALVLLVVPKIYGACRILVWRPWMLSRRFKKQGISGPKYRILYGNLREIRK 117

Query: 68  LLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIKELLSK 127
           + +++    +   S+D+VPR+LPH   W +Q+G+ F++W G +PR+C+++ +L K++LS 
Sbjct: 118 MKNEAKLMVLDPNSNDIVPRVLPHLQQWKSQYGETFLYWQGTDPRLCISDHELAKQILSN 177

Query: 128 YSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTRE 187
                 KS  + +  K   G GL+  NG +W   R I+ PAF  D+LK     MV+CT  
Sbjct: 178 KFVFFSKSKTKPEILK-LSGNGLIFVNGLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFR 237

Query: 188 MLESIENEVKSGRSE--FEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 247
           M    + +     +E    I     RLTADII+   F SSY +G ++F     LQ  CA 
Sbjct: 238 MFLEWKKQRNGVETEQFVLISREFKRLTADIIATAAFGSSYAEGIEVFKSQLELQKCCAA 297

Query: 248 ASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLGMLL 307
           A   L  PG ++ P+  N +I  L  KV   +  II +R        S  YGNDLLG++L
Sbjct: 298 ALTDLYFPGIQYLPTPSNLQIWKLDMKVNSSIKRIIDARL----TSESKDYGNDLLGIML 357

Query: 308 -----NEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKL 367
                NE +KK       +++  I++ECKTFFFAGHETTA LLTW+ MLL+ +  WQ KL
Sbjct: 358 TAASSNESEKK-------MSIDEIIEECKTFFFAGHETTANLLTWSTMLLSLHQDWQEKL 417

Query: 368 RAEVIAICGSE-TPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKG 427
           R EV   CG +  P     SKL L+  V  ESLRLY P   L R+A ED+KLG+LEIPKG
Sbjct: 418 REEVFNECGKDKIPDAETCSKLKLMNTVFMESLRLYGPVLNLLRLASEDMKLGNLEIPKG 477

Query: 428 LSIWIPVLAIHHSEEIWGKDANEFNPERFANS---KAFTSGGFIPFASGPRNCVGQSFAL 487
            +I +P+  +H  + +WG DA++FNP RFAN     A      + F+ GPR C+GQ+FA+
Sbjct: 478 TTIILPIAKMHRDKAVWGSDADKFNPMRFANGLSRAANHPNALLAFSMGPRACIGQNFAI 537

Query: 488 METKIILAMLISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPLN 521
           ME K +LAM++ +F   +S  Y+HAP   LT++P+Y + V L+P++
Sbjct: 538 MEAKTVLAMILQRFRLNLSADYKHAPADHLTLQPQYDLPVILEPID 571

BLAST of Bhi06M000094 vs. ExPASy Swiss-Prot
Match: Q9ASR3 (Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 4.4e-100
Identity = 203/527 (38.52%), Postives = 308/527 (58.44%), Query Frame = 0

Query: 3   IMLPLLLTTLVLLTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNILD 62
           I L L++  L++    F  F +L        + P  +  ++  QG+ GP  R   GN+ +
Sbjct: 5   IFLALIVLILIIGLRIFKAFMIL-------VWHPFVLTRRLKNQGISGPNYRIFYGNLSE 64

Query: 63  VAALLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIKEL 122
           +  +  +S  S +   S+D+ PR+LPH+  W +Q+G+ F++WNG EPR+C+++ +L K +
Sbjct: 65  IKKMKRESHLSILDPSSNDIFPRILPHYQKWMSQYGETFLYWNGTEPRICISDPELAKTM 124

Query: 123 LSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVEC 182
           LS       KS  + +  K    +GL+   G +W   R I+ PAF  DRLK     MV+C
Sbjct: 125 LSNKLGFFVKSKARPEAVKLVGSKGLVFIEGADWVRHRRILNPAFSIDRLKIMTTVMVDC 184

Query: 183 TREML-----ESIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVL 242
           T +ML     ES + E +  + + E+ E   RLTADII+ + F SSY +G ++F     L
Sbjct: 185 TLKMLEEWRKESTKEETEHPKIKKEMNEEFQRLTADIIATSAFGSSYVEGIEVFRSQMEL 244

Query: 243 QHLCAQASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGND 302
           +     +   + +PG+++ P+  N  +  L+ K++  +  II SR     +   S YG+D
Sbjct: 245 KRCYTTSLNQVSIPGTQYLPTPSNIRVWKLERKMDNSIKRIISSR-----LQSKSDYGDD 304

Query: 303 LLGMLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHK 362
           LLG+LL       +G    ++++ I+ EC+TFFF GHETT+ LL WT MLL+ +  WQ K
Sbjct: 305 LLGILLKAY--NTEGKERKMSIEEIIHECRTFFFGGHETTSNLLAWTTMLLSLHQDWQEK 364

Query: 363 LRAEVIAICGSE-TPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPK 422
           LR E+   CG E TP     SKL L+ MVI ESLRLY P S L R A  +IKLGDLEIPK
Sbjct: 365 LREEIFKECGKEKTPDSETFSKLKLMNMVIMESLRLYGPVSALAREASVNIKLGDLEIPK 424

Query: 423 GLSIWIPVLAIHHSEEIWGKDANEFNPERFANS---KAFTSGGFIPFASGPRNCVGQSFA 482
           G ++ IP+L +H  + +WG DA++FNP RFAN     A      + F+ GPR C+GQ+F 
Sbjct: 425 GTTVVIPLLKMHSDKTLWGSDADKFNPMRFANGVSRAANHPNALLAFSVGPRACIGQNFV 484

Query: 483 LMETKIILAMLISKFSF-TISDKYRHAPVVVLTIKPKYGVQVCLKPL 520
           ++E K +L M++ +F F ++ D+Y+H PV  +TI+P+YG+ V L+PL
Sbjct: 485 MIEAKTVLTMILQRFRFISLCDEYKHTPVDNVTIQPQYGLPVMLQPL 517

BLAST of Bhi06M000094 vs. ExPASy Swiss-Prot
Match: O48786 (Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1)

HSP 1 Score: 350.1 bits (897), Expect = 4.2e-95
Identity = 185/515 (35.92%), Postives = 299/515 (58.06%), Query Frame = 0

Query: 14  LLTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNILDVAALLSKSTSS 73
           +L  S  +  ++   +S   + PR+I+   +KQG+RGP     +GN+ ++  ++ K++S 
Sbjct: 13  VLVLSVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASSH 72

Query: 74  DMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVSGKS 133
            MP  SH+++PR+L  +  W   +G  F+ W G   R+ + + DLI+E+ SK        
Sbjct: 73  PMP-FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIFSKSEFYEKNE 132

Query: 134 WLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTREMLESIENE 193
                  K   G GLL   G+ W H R I++P F  + LK     +++   +M++   ++
Sbjct: 133 --AHPLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDK 192

Query: 194 V-KSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHLCLPG 253
           + ++G  E ++ E+   LT D+ISRT F SSYE G+ +F L      LCA+A + + +PG
Sbjct: 193 LSENGEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIPG 252

Query: 254 SRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSS----YGNDLLGMLLNEMQK 313
            RFFP++ N +   L  ++ + L+++I+ RR     G           DLLG+++     
Sbjct: 253 YRFFPTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMI----- 312

Query: 314 KKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKLRAEVIAICGS 373
                  ++ +Q I++ECK+FFFAG +TT+ LLTWT +LL+ +P WQ K R EV+ +CGS
Sbjct: 313 ----QAKNVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGS 372

Query: 374 -ETPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAI 433
            + P+  H+ KL  L M++NESLRLYPP     R A  D+KLG  +IP G  + IP++A+
Sbjct: 373 RDVPTKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAV 432

Query: 434 HHSEEIWGKDANEFNPERFANS---KAFTSGGFIPFASGPRNCVGQSFALMETKIILAML 493
           HH + IWG D NEFNP RFA+     A    GFIPF  G R C+GQ+ A+++ K+ LA++
Sbjct: 433 HHDQAIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVM 492

Query: 494 ISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPL 520
           I +F+F ++  Y+HAP V++ + P++G  +  + L
Sbjct: 493 IQRFTFHLAPTYQHAPTVLMLLYPQHGAPITFRRL 515

BLAST of Bhi06M000094 vs. ExPASy TrEMBL
Match: A0A5A7T7B8 (Cytokinin hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001740 PE=3 SV=1)

HSP 1 Score: 995.7 bits (2573), Expect = 7.1e-287
Identity = 496/522 (95.02%), Postives = 508/522 (97.32%), Query Frame = 0

Query: 1   MPIMLPLLLTTLVLLTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNI 60
           MPIMLPLLLTTL LLTTSFFIFSLLYDT+SCY  TPRRIK KMAKQGV GPKPRPLLGNI
Sbjct: 1   MPIMLPLLLTTLALLTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNI 60

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIK 120
           LDVA+LLSKSTSSDMPSISHDLVPRLLPHFLAWS+QFGKRFIFWNGIEPRMCLTETDLIK
Sbjct: 61  LDVASLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIK 120

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMV 180
           ELLSKYS VSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMV
Sbjct: 121 ELLSKYSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMV 180

Query: 181 ECTREMLESIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240
           ECT+EMLESIE EVKSGRSEFEIGEYMTRLTADIISRTEFESS+EKGKQIFHLLTVLQHL
Sbjct: 181 ECTKEMLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHL 240

Query: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLG 300
           CAQASRHLC PGSRFFPSKYNREIKALKGKVEELLMEIIQSR+DCVEIGRSSSYGNDLLG
Sbjct: 241 CAQASRHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLG 300

Query: 301 MLLNEMQKKK-DG-NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKL 360
           MLLNEMQKKK DG NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP+WQ K+
Sbjct: 301 MLLNEMQKKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDKV 360

Query: 361 RAEVIAICGSETPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420
           RAEV+A+CGSETPSFHHLSKLSLL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL
Sbjct: 361 RAEVMAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420

Query: 421 SIWIPVLAIHHSEEIWGKDANEFNPERFANSKAFTSGGFIPFASGPRNCVGQSFALMETK 480
           SIWIPVLAIHHSEE+WGKDANEFNPERFANSK FTSGGFIPFASGPRNC+GQSFALMETK
Sbjct: 421 SIWIPVLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMETK 480

Query: 481 IILAMLISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPLN 521
           IILAMLISKFSFTISD YRHAPVVVLTIKPKYGVQVCLKPLN
Sbjct: 481 IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of Bhi06M000094 vs. ExPASy TrEMBL
Match: A0A1S3CAS2 (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103498598 PE=3 SV=1)

HSP 1 Score: 994.6 bits (2570), Expect = 1.6e-286
Identity = 495/522 (94.83%), Postives = 508/522 (97.32%), Query Frame = 0

Query: 1   MPIMLPLLLTTLVLLTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNI 60
           MPIMLPLLLTTL LLTTSFFIFSLLYDT+SCY  TPRRIK KMAKQGV GPKPRPLLGNI
Sbjct: 1   MPIMLPLLLTTLALLTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNI 60

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIK 120
           LDVA+LLSKSTSSDMPSISHDLVPRLLPHFLAWS+QFGKRFIFWNGIEPRMCLTETDLIK
Sbjct: 61  LDVASLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIK 120

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMV 180
           ELLSKYS VSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMV
Sbjct: 121 ELLSKYSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMV 180

Query: 181 ECTREMLESIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240
           ECT+EMLESIE EVKSGRSEFEIGEYMTRLTADIISRTEFESS+EKGKQIFHLLTVLQHL
Sbjct: 181 ECTKEMLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHL 240

Query: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLG 300
           CAQASRHLC PGSRFFPSKYNREIKALKGKVEELLMEIIQSR+DCVEIGRSSSYGNDLLG
Sbjct: 241 CAQASRHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLG 300

Query: 301 MLLNEMQKKK-DG-NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKL 360
           MLLNEMQKKK DG NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNP+WQ K+
Sbjct: 301 MLLNEMQKKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDKV 360

Query: 361 RAEVIAICGSETPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420
           RAEV+A+CGSETPSFHHLSKLSLL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL
Sbjct: 361 RAEVMAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGL 420

Query: 421 SIWIPVLAIHHSEEIWGKDANEFNPERFANSKAFTSGGFIPFASGPRNCVGQSFALMETK 480
           SIWIP+LAIHHSEE+WGKDANEFNPERFANSK FTSGGFIPFASGPRNC+GQSFALMETK
Sbjct: 421 SIWIPLLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMETK 480

Query: 481 IILAMLISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPLN 521
           IILAMLISKFSFTISD YRHAPVVVLTIKPKYGVQVCLKPLN
Sbjct: 481 IILAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of Bhi06M000094 vs. ExPASy TrEMBL
Match: A0A6J1FGF0 (cytokinin hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445453 PE=3 SV=1)

HSP 1 Score: 954.5 bits (2466), Expect = 1.8e-274
Identity = 473/519 (91.14%), Postives = 491/519 (94.61%), Query Frame = 0

Query: 1   MPIMLPLLLTTLVLLTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNI 60
           M IML LLLTTL    TSF IFSLLY TLSCY  TPRRI  KMAKQGVRGPKPRPLLGNI
Sbjct: 1   MAIMLALLLTTLAFAATSFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNI 60

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIK 120
           LDVAALLSKSTSSDMPSISHDLVPRLLPH+LAWS QFGKRFIFWNGIEPRMCLTET LIK
Sbjct: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIK 120

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMV 180
           ELLSKYS+VSGKSWLQQQGSKHFIGRGLLMANG NWFHQRHIVAPAFVGDRLKSYAGYMV
Sbjct: 121 ELLSKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMV 180

Query: 181 ECTREMLESIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240
           ECTREML+S+ENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHL
Sbjct: 181 ECTREMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240

Query: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLG 300
           CAQASRHLCLPGSRFFPSKYNREIKALKGKVE LLMEIIQSR+D VEIGRSSSYGNDLLG
Sbjct: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLG 300

Query: 301 MLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKLRA 360
           MLLNEMQKKK+GNGLSLNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQ ++RA
Sbjct: 301 MLLNEMQKKKNGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRVRA 360

Query: 361 EVIAICGSETPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSI 420
           E+ A+C S+TPS HHLSKL LLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS+
Sbjct: 361 ELNAVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSV 420

Query: 421 WIPVLAIHHSEEIWGKDANEFNPERFANSKAFTSGGFIPFASGPRNCVGQSFALMETKII 480
           WIPV+AIHHSEEIWGKDANEFNPERF +SKAFT GGFIPFASGPRNCVGQ+FALMETKII
Sbjct: 421 WIPVVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETKII 480

Query: 481 LAMLISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPL 520
           LAMLISKF FTIS  YRHAPVVVLT+KPKYGVQ+CL PL
Sbjct: 481 LAMLISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPL 519

BLAST of Bhi06M000094 vs. ExPASy TrEMBL
Match: A0A6J1JYD2 (cytokinin hydroxylase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488993 PE=3 SV=1)

HSP 1 Score: 952.6 bits (2461), Expect = 6.9e-274
Identity = 472/519 (90.94%), Postives = 490/519 (94.41%), Query Frame = 0

Query: 1   MPIMLPLLLTTLVLLTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNI 60
           M  ML LLLTTL    TSF IFSLLY TLSCY  TPRRI  KMAKQGVRGPKPRPLLGNI
Sbjct: 1   MATMLALLLTTLAFAVTSFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNI 60

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIK 120
           LDVAALLSKSTSSDMPSISHDLVPRLLPH+LAWS QFGKRFIFWNGIEPRMCLTET LIK
Sbjct: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIK 120

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMV 180
           ELLSKYS+VSGKSWLQQQGSKHFIGRGLLMANG NWFHQRHIVAPAFVGDRLKSYAGYMV
Sbjct: 121 ELLSKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMV 180

Query: 181 ECTREMLESIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240
           ECTREML+S+ENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHL
Sbjct: 181 ECTREMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240

Query: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLG 300
           CAQASRHLCLPGSRFFPSKYNREIKALKGKVE LLMEIIQSR+D VEIGRSSSYGNDLLG
Sbjct: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLG 300

Query: 301 MLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKLRA 360
           MLLNEMQKKK+GNGLSLNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQ ++RA
Sbjct: 301 MLLNEMQKKKNGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRVRA 360

Query: 361 EVIAICGSETPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSI 420
           E+ A+C S+TPS HHLSKL LLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS+
Sbjct: 361 ELNAVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSV 420

Query: 421 WIPVLAIHHSEEIWGKDANEFNPERFANSKAFTSGGFIPFASGPRNCVGQSFALMETKII 480
           WIPV+AIHHSEEIWGKDANEFNPERF +SKAFT GGFIPFASGPRNCVGQ+FALMETKII
Sbjct: 421 WIPVVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETKII 480

Query: 481 LAMLISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPL 520
           LAMLISKF FTIS  YRHAPVVVLT+KPKYGVQ+CL PL
Sbjct: 481 LAMLISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPL 519

BLAST of Bhi06M000094 vs. ExPASy TrEMBL
Match: A0A6J1FHA5 (cytokinin hydroxylase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445453 PE=3 SV=1)

HSP 1 Score: 929.1 bits (2400), Expect = 8.2e-267
Identity = 465/519 (89.60%), Postives = 483/519 (93.06%), Query Frame = 0

Query: 1   MPIMLPLLLTTLVLLTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNI 60
           M IML LLLTTL    TSF IFSLLY TLSCY  TPRRI  KMAKQGVRGPKPRPLLGNI
Sbjct: 1   MAIMLALLLTTLAFAATSFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNI 60

Query: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIK 120
           LDVAALLSKSTSSDMPSISHDLVPRLLPH+LAWS QFGKRFIFWNGIEPRMCLTET LIK
Sbjct: 61  LDVAALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIK 120

Query: 121 ELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMV 180
           ELLSKYS+VSGKSWLQQQGSKHFIGRGLLMANG NWFHQRHIVAPAFVGDRLKSYAGYMV
Sbjct: 121 ELLSKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMV 180

Query: 181 ECTREMLESIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240
           ECTREML+S+ENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHL
Sbjct: 181 ECTREMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHL 240

Query: 241 CAQASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLG 300
           CAQASRHLCLPGS        REIKALKGKVE LLMEIIQSR+D VEIGRSSSYGNDLLG
Sbjct: 241 CAQASRHLCLPGS--------REIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLG 300

Query: 301 MLLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKLRA 360
           MLLNEMQKKK+GNGLSLNLQIIMDECKTFFFAGHETTALLLTWT+MLLATNPSWQ ++RA
Sbjct: 301 MLLNEMQKKKNGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRVRA 360

Query: 361 EVIAICGSETPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSI 420
           E+ A+C S+TPS HHLSKL LLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS+
Sbjct: 361 ELNAVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSV 420

Query: 421 WIPVLAIHHSEEIWGKDANEFNPERFANSKAFTSGGFIPFASGPRNCVGQSFALMETKII 480
           WIPV+AIHHSEEIWGKDANEFNPERF +SKAFT GGFIPFASGPRNCVGQ+FALMETKII
Sbjct: 421 WIPVVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETKII 480

Query: 481 LAMLISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPL 520
           LAMLISKF FTIS  YRHAPVVVLT+KPKYGVQ+CL PL
Sbjct: 481 LAMLISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPL 511

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT5G38450.13.7e-21169.31cytochrome P450, family 735, subfamily A, polypeptide 1 [more]
AT1G67110.11.1e-20467.76cytochrome P450, family 735, subfamily A, polypeptide 2 [more]
AT2G46950.12.4e-10139.16cytochrome P450, family 709, subfamily B, polypeptide 2 [more]
AT2G46960.23.1e-10138.52cytochrome P450, family 709, subfamily B, polypeptide 1 [more]
AT2G26710.13.0e-9635.92Cytochrome P450 superfamily protein [more]
Match NameE-valueIdentityDescription
Q9FF185.2e-21069.31Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1[more]
Q9ZW951.6e-20367.76Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1[more]
F4IK453.4e-10039.16Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1[more]
Q9ASR34.4e-10038.52Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1[more]
O487864.2e-9535.92Cytochrome P450 734A1 OS=Arabidopsis thaliana OX=3702 GN=CYP734A1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A5A7T7B87.1e-28795.02Cytokinin hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
A0A1S3CAS21.6e-28694.83cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103498598 PE=3 SV=1[more]
A0A6J1FGF01.8e-27491.14cytokinin hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445... [more]
A0A6J1JYD26.9e-27490.94cytokinin hydroxylase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11148899... [more]
A0A6J1FHA58.2e-26789.60cytokinin hydroxylase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 264..284
NoneNo IPR availablePANTHERPTHR24282:SF186CYTOCHROME P450 FAMILY PROTEINcoord: 8..516
NoneNo IPR availablePANTHERPTHR24282CYTOCHROME P450 FAMILY MEMBERcoord: 8..516
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 94..113
score: 22.54
coord: 321..338
score: 29.73
coord: 457..467
score: 49.55
coord: 467..490
score: 31.04
coord: 424..448
score: 25.82
coord: 210..228
score: 19.27
coord: 341..367
score: 28.59
IPR001128Cytochrome P450PRINTSPR00385P450coord: 467..478
score: 42.46
coord: 458..467
score: 55.03
coord: 332..349
score: 40.96
coord: 384..395
score: 46.75
IPR001128Cytochrome P450PFAMPF00067p450coord: 95..495
e-value: 4.7E-85
score: 286.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 32..522
e-value: 1.4E-112
score: 378.7
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 50..518

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Bhi06G000094Bhi06G000094gene


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi06M000094.utr5p1Bhi06M000094.utr5p1five_prime_UTR


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi06M000094.exon1Bhi06M000094.exon1exon
Bhi06M000094.exon2Bhi06M000094.exon2exon
Bhi06M000094.exon3Bhi06M000094.exon3exon
Bhi06M000094.exon4Bhi06M000094.exon4exon
Bhi06M000094.exon5Bhi06M000094.exon5exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Bhi06M000094cds.Bhi06M000094CDS
cds.Bhi06M000094cds.Bhi06M000094_2CDS
cds.Bhi06M000094cds.Bhi06M000094_3CDS
cds.Bhi06M000094cds.Bhi06M000094_4CDS
cds.Bhi06M000094cds.Bhi06M000094_5CDS


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi06M000094.utr3p1Bhi06M000094.utr3p1three_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Bhi06M000094Bhi06M000094-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen