Bhi05M000669 (mRNA) Wax gourd (B227) v1

Overview


Name	Bhi05M000669
Type	mRNA
Organism	Benincasa hispida (Wax gourd (B227) v1)
Description	Heavy metal-associated isoprenylated plant protein 3-like
Location	chr5: 27785793 .. 27788761 (+)
Sequence length	868
RNA-Seq Expression	Bhi05M000669
Synteny	Bhi05M000669

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAAAGACAACCTCAATAACTTCATCATATAAATCTGTCTCTGCCTCTGTTTCTTCTATTACTTTACCATATTAACCATGAAGAAGCTTGGTGGATTCATGTGCCACTCTCCAGCAGCCACCGCAGTCTGCATCCCCGCCGATCCCCGCTCCGCAGTCGTGTCGAGAAGGCTGGCTAGAGCTGACTATGTTCATAATGCCAATAGCAACCGTGCGAGGTTGGTGAGCAATGCTGTGAAGTACTCGAAGCTTGTCGAGTCGACGAACTTGAGTGTTCGGAGCATGGAAACGCAGACCAACATGAAGAAATCAGTGCCGGTCATGCTTTCTCCGCCGCCGGAGAATGTCTTCCATGTGGTGGTTATGAGGGTGGCGCTTCACTGTCAAGGCTGTGCTAGTAAAGTTAAGAGACATCTCGCCAAAATGGAAGGTATAGTAAATCATATTCATGATATTATTTCTATTTTGTAGGTTCAAGATTAAAATTTCAATTGTATGGATATAATATTTTCTGACCCTCACGAAATTTCAAAAATTAATGATTTATTCTTAATATTGATGTTAACTCTCCCATATAAGATGTATGGAAGGATGCATCAACGTATAACCGCCAAATATTTAGTATAATTAAAGTTTTTCTTTATAATAAAAGTGGTATTATTATTATTATTATTATTTGCTAAAAAATTTTACACTATAAAGCAAAATATGCCCAACAAGCATATACTTATTTTCTCTTATTTTCTAACTTTTGATCATGGTAGAAAACATCTTAGTTATATTTTAAGATTTATGTTTAAATAGTTAGGCTCCATCTTATCACACTTTTTTTAAAATTAAGTCTATTTCCTCCACATTTCTTAAAATAATTTATATTTTTAAAAAATACAATGGTTGAATTCTTAGTCAAATTCTAAAAACAAAAATAATTTTTTTTTTAGTTTTCAAAATTTAGCGTATTTTTTAAATTATTGGTAAAAAGTAGATAACAAATAAAGAAATTCGGAGATGAAAATAATGTTTATAGACTTAATTTAAAAAAAAAAAAAACTGAATGGTTACGAGTGCCTTAATCATTCTCTTAGTTTCATTACTAGTGCTTTTTTTAAATAAAAAGAAATCATCATTTTTTATTGAAAAATTCTTATAAATAGAAAAATCTCAAAAATATTTATACTAAAAAATTTCAAAAGTTTTATATACTTTTAGAACATTTTACTATAAACTTTAAATATTTTTAAATAATTTTTTTATATTTAAAAAGTTCCTTGTTTATTCTACATATATGTAAAACTTATTATTGCTACACTAGATTTAACCACGTTCAAAAGTTTCTCCTTTTGTAATAAATACATATATAATATTATGTTTTTCATTGGTTTCCAACCGAGATTTTGACCTTAATTTTCGAGTTTAGTAATTGCTAAAACTTAAAAGAAAAAAAAAACTAAAATAGAACCATTTAAGGAACGTTGGATAAACAATTGGATATTTTAGTTTTAGATTGTTATATTTGAGTTATAATAAATTGTGTTTAGAGGGTAGATTATTTTAGTTTGAATTATAATACTATGTGTTTGAAGTGTAAACTACTTTAGTTCGAGAAAGAAATAATAAACAATATAACAAAGAATAAAAAATTGATGAAGGTAAAATTAGAGGATTCAAAAAATCTGATGACCCGAAAAAACCAATTAACCCAACCCAATCCGTGCGATTTGGGTTTGATTATCAACTCATTTGGGTCCGGTTGGATTCAAATAAATGAAAATTTTATCGATTAAGTTGGCTCATGGGTTCACCTAATATAATTCAAACCAACCCGAATTTATTATTTACTTTAAAATATATTTTTTTATTACATATAACACAATTATTTATACATATATTGATTTTAATTTTATTTAATTTCAATAATTTTTTTAATAATTTATTCTTCAACAACTCTTAAAAGTAGTTTATTTGCATTTTAGAAAGAAAATTTTCACTATATAAATTAAAATTGAGTTGTTAATCTTATTTAAAATTTTATTTTAATATATAAAAATAATTAAATAAGATTTATGTATATATATACATTGGGCTTCTTTTTAGAACAAAATTTTAAACAAATGACCCGATTAACCCGAACCAACCCAACCCAAATATTTCATGGTTGGATTGGGTTGGGTTCATTTTTTAATAAAGATTATTTGGATTGAAGAAATTTACAATCCCAACAGTTGGATTGGGTCTAAAAAGTTTATCAACCCAATCCAATCCAACTCATGAACACCATAGGTAAAATAGTAAAAGTCATAACAAATAATAAATACGGTAGTAAATGAAGGTTTTGAAATACTAGTTAATTATAGCTAATTCGAAAATTAAAAAATAATATTTATTATCACTTGATTATTATATTCTTATAATAGTCTTTCCTTCAAACGTATACCTAAGTTTCCAGTGAAAAAAAAAATGTAAAACAAGAATATTTTGGGATGAAAGTGAAATTCATATATCTTTTTACGACTGTAATTGGCAATTGACAGGAGTGACGTCATTCAGCATAGATCTTGAGGCCAAGAGAGTGACTGTGATGGGACATATTTCACCGCTGGGTGTTTTGGAGAGTATTTCTAAGGTGAAAAGAGCCGAGTTATGGCCAATGTTAAACTAATCACTTTATAATTAAATGGCTTCTGGCTGTGACAGAACAGTGACTGTGAGAGAGAATATGATTGAAATGACCAAAATGGGACACCAAAAGCAAGAAAATGTGTGTAATTTGTAAGATCCCAGTAGGAGTTATGTGTAATTTCAATCTTTTTGTTGGACAAATTATCCATTTTATTAATGTTGGTCCTAAATTAATTTAAGAGTTAGTGGGTGGATATGTTACTAGTTCATCATTTGGTTTTATGAATTGGAATCAACTTTTCTTATGATGTTAGTGATATTTTTGCCTTAATATTATAAATTTTCCTCCC

mRNA sequence

GAAAAAGACAACCTCAATAACTTCATCATATAAATCTGTCTCTGCCTCTGTTTCTTCTATTACTTTACCATATTAACCATGAAGAAGCTTGGTGGATTCATGTGCCACTCTCCAGCAGCCACCGCAGTCTGCATCCCCGCCGATCCCCGCTCCGCAGTCGTGTCGAGAAGGCTGGCTAGAGCTGACTATGTTCATAATGCCAATAGCAACCGTGCGAGGTTGGTGAGCAATGCTGTGAAGTACTCGAAGCTTGTCGAGTCGACGAACTTGAGTGTTCGGAGCATGGAAACGCAGACCAACATGAAGAAATCAGTGCCGGTCATGCTTTCTCCGCCGCCGGAGAATGTCTTCCATGTGGTGGTTATGAGGGTGGCGCTTCACTGTCAAGGCTGTGCTAGTAAAGTTAAGAGACATCTCGCCAAAATGGAAGGAGTGACGTCATTCAGCATAGATCTTGAGGCCAAGAGAGTGACTGTGATGGGACATATTTCACCGCTGGGTGTTTTGGAGAGTATTTCTAAGGTGAAAAGAGCCGAGTTATGGCCAATGTTAAACTAATCACTTTATAATTAAATGGCTTCTGGCTGTGACAGAACAGTGACTGTGAGAGAGAATATGATTGAAATGACCAAAATGGGACACCAAAAGCAAGAAAATGTGTGTAATTTGTAAGATCCCAGTAGGAGTTATGTGTAATTTCAATCTTTTTGTTGGACAAATTATCCATTTTATTAATGTTGGTCCTAAATTAATTTAAGAGTTAGTGGGTGGATATGTTACTAGTTCATCATTTGGTTTTATGAATTGGAATCAACTTTTCTTATGATGTTAGTGATATTTTTGCCTTAATATTATAAATTTTCCTCCC

Coding sequence (CDS)

ATGAAGAAGCTTGGTGGATTCATGTGCCACTCTCCAGCAGCCACCGCAGTCTGCATCCCCGCCGATCCCCGCTCCGCAGTCGTGTCGAGAAGGCTGGCTAGAGCTGACTATGTTCATAATGCCAATAGCAACCGTGCGAGGTTGGTGAGCAATGCTGTGAAGTACTCGAAGCTTGTCGAGTCGACGAACTTGAGTGTTCGGAGCATGGAAACGCAGACCAACATGAAGAAATCAGTGCCGGTCATGCTTTCTCCGCCGCCGGAGAATGTCTTCCATGTGGTGGTTATGAGGGTGGCGCTTCACTGTCAAGGCTGTGCTAGTAAAGTTAAGAGACATCTCGCCAAAATGGAAGGAGTGACGTCATTCAGCATAGATCTTGAGGCCAAGAGAGTGACTGTGATGGGACATATTTCACCGCTGGGTGTTTTGGAGAGTATTTCTAAGGTGAAAAGAGCCGAGTTATGGCCAATGTTAAACTAA

Protein sequence

MKKLGGFMCHSPAATAVCIPADPRSAVVSRRLARADYVHNANSNRARLVSNAVKYSKLVESTNLSVRSMETQTNMKKSVPVMLSPPPENVFHVVVMRVALHCQGCASKVKRHLAKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKVKRAELWPMLN

Homology

[Close]

BLAST of Bhi05M000669 vs. TAIR 10
Match: AT2G28660.1 (Chloroplast-targeted copper chaperone protein )

HSP 1 Score: 102.4 bits (254), Expect = 3.3e-22
Identity = 48/96 (50.00%), Postives = 72/96 (75.00%), Query Frame = 0


Query: 61  STNLSVRSMETQTNMKKSVPVMLSPPPENVFHVVVMRVALHCQGCASKVKRHLAKMEGVT 120
           +++ S  S  + ++   SV  + SP P     VVV+RV++HC+GC  KV++H++KMEGVT
Sbjct: 152 TSSASSSSSSSSSSFSSSVTNVSSPAPSTDDQVVVLRVSIHCKGCEGKVRKHISKMEGVT 211

Query: 121 SFSIDLEAKRVTVMGHISPLGVLESISKVKRAELWP 157
           S++IDL  K+VTV+G I+P+G++ESISKVK A+LWP
Sbjct: 212 SYTIDLATKKVTVVGKITPVGLVESISKVKFAQLWP 247

[Close]

BLAST of Bhi05M000669 vs. TAIR 10
Match: AT5G02600.2 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 96.7 bits (239), Expect = 1.8e-20
Identity = 46/75 (61.33%), Postives = 60/75 (80.00%), Query Frame = 0


Query: 84  SPPPENVF--HVVVMRVALHCQGCASKVKRHLAKMEGVTSFSIDLEAKRVTVMGHISPLG 143
           SPP +N     VVV+RV+LHC+GCA KVK+HL+K++GVTS++ID  AK+VTV G ++PL 
Sbjct: 240 SPPEKNSSSDQVVVLRVSLHCKGCAGKVKKHLSKLKGVTSYNIDFAAKKVTVTGDVTPLT 299

Query: 144 VLESISKVKRAELWP 157
           VL SISKVK A+ WP
Sbjct: 300 VLASISKVKNAQFWP 314

[Close]

BLAST of Bhi05M000669 vs. TAIR 10
Match: AT5G02600.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 96.7 bits (239), Expect = 1.8e-20
Identity = 46/75 (61.33%), Postives = 60/75 (80.00%), Query Frame = 0


Query: 84  SPPPENVF--HVVVMRVALHCQGCASKVKRHLAKMEGVTSFSIDLEAKRVTVMGHISPLG 143
           SPP +N     VVV+RV+LHC+GCA KVK+HL+K++GVTS++ID  AK+VTV G ++PL 
Sbjct: 240 SPPEKNSSSDQVVVLRVSLHCKGCAGKVKKHLSKLKGVTSYNIDFAAKKVTVTGDVTPLT 299

Query: 144 VLESISKVKRAELWP 157
           VL SISKVK A+ WP
Sbjct: 300 VLASISKVKNAQFWP 314

[Close]

BLAST of Bhi05M000669 vs. TAIR 10
Match: AT2G37390.1 (Chloroplast-targeted copper chaperone protein )

HSP 1 Score: 90.9 bits (224), Expect = 1.0e-18
Identity = 40/63 (63.49%), Postives = 54/63 (85.71%), Query Frame = 0


Query: 93  VVVMRVALHCQGCASKVKRHLAKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKVKRA 152
           VVV++V+LHC+GC  KV++HLA+M+GVTSF+ID  AK+VTV G I+PL +L+SISKVK A
Sbjct: 182 VVVLKVSLHCRGCEGKVRKHLARMQGVTSFNIDFAAKKVTVTGDITPLEILDSISKVKNA 241

Query: 153 ELW 156
           + W
Sbjct: 242 QFW 244

[Close]

BLAST of Bhi05M000669 vs. TAIR 10
Match: AT2G37390.2 (Chloroplast-targeted copper chaperone protein )

HSP 1 Score: 90.9 bits (224), Expect = 1.0e-18
Identity = 40/63 (63.49%), Postives = 54/63 (85.71%), Query Frame = 0


Query: 93  VVVMRVALHCQGCASKVKRHLAKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKVKRA 152
           VVV++V+LHC+GC  KV++HLA+M+GVTSF+ID  AK+VTV G I+PL +L+SISKVK A
Sbjct: 181 VVVLKVSLHCRGCEGKVRKHLARMQGVTSFNIDFAAKKVTVTGDITPLEILDSISKVKNA 240

Query: 153 ELW 156
           + W
Sbjct: 241 QFW 243

[Close]

BLAST of Bhi05M000669 vs. ExPASy Swiss-Prot
Match: Q8LDS4 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=NAKR1 PE=1 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 2.6e-19
Identity = 46/75 (61.33%), Postives = 60/75 (80.00%), Query Frame = 0


Query: 84  SPPPENVF--HVVVMRVALHCQGCASKVKRHLAKMEGVTSFSIDLEAKRVTVMGHISPLG 143
           SPP +N     VVV+RV+LHC+GCA KVK+HL+K++GVTS++ID  AK+VTV G ++PL 
Sbjct: 240 SPPEKNSSSDQVVVLRVSLHCKGCAGKVKKHLSKLKGVTSYNIDFAAKKVTVTGDVTPLT 299

Query: 144 VLESISKVKRAELWP 157
           VL SISKVK A+ WP
Sbjct: 300 VLASISKVKNAQFWP 314

[Close]

BLAST of Bhi05M000669 vs. ExPASy Swiss-Prot
Match: Q58FZ0 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 2 OS=Arabidopsis thaliana OX=3702 GN=NAKR2 PE=2 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 1.4e-17
Identity = 40/63 (63.49%), Postives = 54/63 (85.71%), Query Frame = 0


Query: 93  VVVMRVALHCQGCASKVKRHLAKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKVKRA 152
           VVV++V+LHC+GC  KV++HLA+M+GVTSF+ID  AK+VTV G I+PL +L+SISKVK A
Sbjct: 182 VVVLKVSLHCRGCEGKVRKHLARMQGVTSFNIDFAAKKVTVTGDITPLEILDSISKVKNA 241

Query: 153 ELW 156
           + W
Sbjct: 242 QFW 244

[Close]

BLAST of Bhi05M000669 vs. ExPASy Swiss-Prot
Match: Q8RXH8 (Protein SODIUM POTASSIUM ROOT DEFECTIVE 3 OS=Arabidopsis thaliana OX=3702 GN=NAKR3 PE=2 SV=1)

HSP 1 Score: 83.2 bits (204), Expect = 2.9e-15
Identity = 40/65 (61.54%), Postives = 52/65 (80.00%), Query Frame = 0


Query: 93  VVVMRVAL--HCQGCASKVKRHLAKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKVK 152
           VVV+RV+L  HC+GC  KVK+HL+KM+GVTSF+ID  +K+VTV G I+PL VL  +SKVK
Sbjct: 169 VVVLRVSLHCHCRGCQGKVKKHLSKMQGVTSFNIDFASKKVTVTGDITPLEVLGCLSKVK 228

Query: 153 RAELW 156
            A+ W
Sbjct: 229 NAQFW 233

[Close]

BLAST of Bhi05M000669 vs. ExPASy Swiss-Prot
Match: O82089 (Copper transport protein CCH OS=Arabidopsis thaliana OX=3702 GN=CCH PE=1 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 2.2e-10
Identity = 30/65 (46.15%), Postives = 44/65 (67.69%), Query Frame = 0


Query: 94  VVMRVALHCQGCASKVKRHLAKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKV-KRA 153
           VV++V + CQGC   V R L KMEGV SF ID++ ++VTV G++ P  V +++SK  K+ 
Sbjct: 5   VVLKVGMSCQGCVGAVNRVLGKMEGVESFDIDIKEQKVTVKGNVEPEAVFQTVSKTGKKT 64

Query: 154 ELWPM 158
             WP+
Sbjct: 65  SYWPV 69

[Close]

BLAST of Bhi05M000669 vs. ExPASy Swiss-Prot
Match: Q94BT9 (Copper transport protein ATX1 OS=Arabidopsis thaliana OX=3702 GN=ATX1 PE=1 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 2.8e-10
Identity = 30/63 (47.62%), Postives = 45/63 (71.43%), Query Frame = 0


Query: 94  VVMRVALHCQGCASKVKRHLAKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKV-KRA 153
           VV+RVA+ C+GC   VKR L KMEGV SF +D++ ++VTV G++ P  VL++++K  K+ 
Sbjct: 5   VVLRVAMTCEGCVGAVKRVLGKMEGVESFDVDIKEQKVTVKGNVQPDAVLQTVTKTGKKT 64

Query: 154 ELW 156
             W
Sbjct: 65  AFW 67

[Close]

BLAST of Bhi05M000669 vs. ExPASy TrEMBL
Match: A0A5A7SQB0 (Heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G002650 PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 9.7e-62
Identity = 137/163 (84.05%), Postives = 145/163 (88.96%), Query Frame = 0


Query: 1   MKKLGGFMCHSPAATAVCIPADPRSAVVSRRLARADYVHN---ANSNRARLVSNAVKYSK 60
           MKKLGGF CHS A TAVCIPADPRSAVVSRRLARADYV+N    N NRARLVSNAVKYSK
Sbjct: 1   MKKLGGFTCHSAATTAVCIPADPRSAVVSRRLARADYVNNNHANNGNRARLVSNAVKYSK 60

Query: 61  LVEST-NLSVRSMETQTNMKK-SVPVMLSPPPE--NVFHVVVMRVALHCQGCASKVKRHL 120
           LVES+ +LSVRS   Q N+KK ++PVMLS P E  NVFHVVVMRVALHCQGCASKVKRHL
Sbjct: 61  LVESSPSLSVRSEGKQINLKKPAMPVMLSSPAENNNVFHVVVMRVALHCQGCASKVKRHL 120

Query: 121 AKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKVKRAELWP 157
           +KMEGVTSFSIDLEAKRVTVMGHISPLGVL+SISKVKRAELWP
Sbjct: 121 SKMEGVTSFSIDLEAKRVTVMGHISPLGVLQSISKVKRAELWP 163

[Close]

BLAST of Bhi05M000669 vs. ExPASy TrEMBL
Match: A0A6J1HVM5 (heavy metal-associated isoprenylated plant protein 35-like OS=Cucurbita maxima OX=3661 GN=LOC111467075 PE=4 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 2.1e-56
Identity = 123/158 (77.85%), Postives = 133/158 (84.18%), Query Frame = 0


Query: 1   MKKLGGFMCHSPAATAVCIPADPRSAVVSRRLARADYVHNANSNRARLVSNAVKYSKLVE 60
           MKKLGGFMCHSPAATAVCIPADPRSAVVS  LARA+ + N N  R RLV+NAVKYSKL +
Sbjct: 1   MKKLGGFMCHSPAATAVCIPADPRSAVVSTSLARANNLKNVNGIRVRLVNNAVKYSKLGK 60

Query: 61  STNLSVRSMETQTNMKKSVPVMLS---PPPENVFHVVVMRVALHCQGCASKVKRHLAKME 120
           S      S  T  ++KK V +MLS   PPPENVFHVVVM+VALHCQGCASKVKRHL KME
Sbjct: 61  SP-----SAGTTNDVKKPVALMLSSPPPPPENVFHVVVMKVALHCQGCASKVKRHLTKME 120

Query: 121 GVTSFSIDLEAKRVTVMGHISPLGVLESISKVKRAELW 156
           GVTSFSIDLEAK+VTVMGHISPLGVLES+SKVKRAELW
Sbjct: 121 GVTSFSIDLEAKKVTVMGHISPLGVLESLSKVKRAELW 153

[Close]

BLAST of Bhi05M000669 vs. ExPASy TrEMBL
Match: A0A0A0K2I2 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G060180 PE=4 SV=1)

HSP 1 Score: 224.9 bits (572), Expect = 2.3e-55
Identity = 127/164 (77.44%), Postives = 139/164 (84.76%), Query Frame = 0


Query: 1   MKKLGGFMCHSPAATAVCIPADPRSAVVSRRLARADYVHNANSNR----ARLVS-NAVKY 60
           MKKLGGFMCHS A TAVCIP+DPRS VVSRRLAR DY +N ++N     AR VS N VKY
Sbjct: 1   MKKLGGFMCHSAATTAVCIPSDPRSTVVSRRLARPDYANNNHANNGNRSARFVSNNTVKY 60

Query: 61  SKLVEST-NLSVRSMETQTNMKK-SVPVMLSPPPE--NVFHVVVMRVALHCQGCASKVKR 120
           SKLVES+ +LSVRS     N+KK ++PVMLS P E  NVFHVVVMRVALHCQGCASKVKR
Sbjct: 61  SKLVESSPSLSVRSEGKHINLKKPAMPVMLSSPAENNNVFHVVVMRVALHCQGCASKVKR 120

Query: 121 HLAKMEGVTSFSIDLEAKRVTVMGHISPLGVLESISKVKRAELW 156
           HL++MEGVTSFSIDLEAKRVTVMGHISPLGVL+SISKVKRAELW
Sbjct: 121 HLSRMEGVTSFSIDLEAKRVTVMGHISPLGVLQSISKVKRAELW 164

[Close]

BLAST of Bhi05M000669 vs. ExPASy TrEMBL
Match: A0A6J1HJ48 (protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like OS=Cucurbita moschata OX=3662 GN=LOC111464027 PE=4 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 9.1e-52
Identity = 121/154 (78.57%), Postives = 130/154 (84.42%), Query Frame = 0


Query: 8   MCHSPAATAVCIPADPRSAVVSRR-LARADYVHNANS--NRARLVSNAVKYSKLVESTNL 67
           MC SPAATAVCIPADPRSAVVSRR LARA+ + NAN+  NR RLVSNAVKYSKL +S   
Sbjct: 1   MCQSPAATAVCIPADPRSAVVSRRSLARANSLKNANANGNRVRLVSNAVKYSKLRKSP-- 60

Query: 68  SVRSMETQTNMKKSVPVMLS---PPPENVFHVVVMRVALHCQGCASKVKRHLAKMEGVTS 127
              S  T  ++KK V +MLS   PPPENVFHVVVM+VALHCQGCASKVKRHL KMEGVTS
Sbjct: 61  ---SAGTTNDVKKPVTLMLSSPPPPPENVFHVVVMKVALHCQGCASKVKRHLTKMEGVTS 120

Query: 128 FSIDLEAKRVTVMGHISPLGVLESISKVKRAELW 156
           FSIDLEAKRVTVMGHISPLGVLES+SKVKRAELW
Sbjct: 121 FSIDLEAKRVTVMGHISPLGVLESLSKVKRAELW 149

[Close]

BLAST of Bhi05M000669 vs. ExPASy TrEMBL
Match: A0A6J1DJK2 (protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like OS=Momordica charantia OX=3673 GN=LOC111021696 PE=4 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 4.7e-48
Identity = 116/158 (73.42%), Postives = 124/158 (78.48%), Query Frame = 0


Query: 2   KKLGGFMCHSPAATAVCIPADPRSA-VVSRRLARADYVHNANS-NRARLVSNAVKYSKLV 61
           KKLGGF CHSP ATAVCI A+PRSA VV RRLARAD   NAN  +R R  SN V Y +LV
Sbjct: 3   KKLGGFTCHSPTATAVCISANPRSAVVVPRRLARADV--NANGVDRVRFASNGVNYLRLV 62

Query: 62  ESTNLSVRSMETQTNMKKSVPVMLSPPPENVFHVVVMRVALHCQGCASKVKRHLAKMEGV 121
            S +LS+RS      +K+ V   LS  PENVF VVV+RVALHCQGCASKVKRHL KMEGV
Sbjct: 63  ASPSLSIRSEGRLPAVKQPVST-LSSSPENVFQVVVLRVALHCQGCASKVKRHLTKMEGV 122

Query: 122 TSFSIDLEAKRVTVMGHISPLGVLESISKVKRAELWPM 158
           TSFSIDLEAKRVTVMG ISP GVLESISKVKRAELWPM
Sbjct: 123 TSFSIDLEAKRVTVMGRISPHGVLESISKVKRAELWPM 157

The following BLAST results are available for this feature:

BLAST of Bhi05M000669 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28660.1	3.3e-22	50.00	Chloroplast-targeted copper chaperone protein	[more]
AT5G02600.2	1.8e-20	61.33	Heavy metal transport/detoxification superfamily protein	[more]
AT5G02600.1	1.8e-20	61.33	Heavy metal transport/detoxification superfamily protein	[more]
AT2G37390.1	1.0e-18	63.49	Chloroplast-targeted copper chaperone protein	[more]
AT2G37390.2	1.0e-18	63.49	Chloroplast-targeted copper chaperone protein	[more]

BLAST of Bhi05M000669 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8LDS4	2.6e-19	61.33	Protein SODIUM POTASSIUM ROOT DEFECTIVE 1 OS=Arabidopsis thaliana OX=3702 GN=NAK...	[more]
Q58FZ0	1.4e-17	63.49	Protein SODIUM POTASSIUM ROOT DEFECTIVE 2 OS=Arabidopsis thaliana OX=3702 GN=NAK...	[more]
Q8RXH8	2.9e-15	61.54	Protein SODIUM POTASSIUM ROOT DEFECTIVE 3 OS=Arabidopsis thaliana OX=3702 GN=NAK...	[more]
O82089	2.2e-10	46.15	Copper transport protein CCH OS=Arabidopsis thaliana OX=3702 GN=CCH PE=1 SV=1	[more]
Q94BT9	2.8e-10	47.62	Copper transport protein ATX1 OS=Arabidopsis thaliana OX=3702 GN=ATX1 PE=1 SV=2	[more]

BLAST of Bhi05M000669 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SQB0	9.7e-62	84.05	Heavy metal-associated isoprenylated plant protein 3-like OS=Cucumis melo var. m...	[more]
A0A6J1HVM5	2.1e-56	77.85	heavy metal-associated isoprenylated plant protein 35-like OS=Cucurbita maxima O...	[more]
A0A0A0K2I2	2.3e-55	77.44	HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G060180 PE=4 SV...	[more]
A0A6J1HJ48	9.1e-52	78.57	protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1DJK2	4.7e-48	73.42	protein SODIUM POTASSIUM ROOT DEFECTIVE 1-like OS=Momordica charantia OX=3673 GN...	[more]

InterPro

Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 96..147 e-value: 9.8E-14 score: 51.5
IPR006121	Heavy metal-associated domain, HMA	PROSITE	PS50846	HMA_2	coord: 92..149 score: 11.337312
IPR006121	Heavy metal-associated domain, HMA	CDD	cd00371	HMA	coord: 100..148 e-value: 7.40301E-12 score: 55.3045
None	No IPR available	GENE3D	3.30.70.100		coord: 90..153 e-value: 1.0E-16 score: 62.9
None	No IPR available	PANTHER	PTHR46119:SF8	OS08G0405700 PROTEIN	coord: 5..156
IPR044526	Protein SODIUM POTASSIUM ROOT DEFECTIVE 1/2/3	PANTHER	PTHR46119	OS08G0405700 PROTEIN	coord: 5..156
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 92..150

Relationships

This mRNA is a part of the following gene feature(s):


Feature Name	Unique Name	Type
Bhi05G000669	Bhi05G000669	gene

The following five_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi05M000669.utr5p1	Bhi05M000669.utr5p1	five_prime_UTR

The following exon feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi05M000669.exon1	Bhi05M000669.exon1	exon
Bhi05M000669.exon2	Bhi05M000669.exon2	exon

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
cds.Bhi05M000669	cds.Bhi05M000669	CDS
cds.Bhi05M000669	cds.Bhi05M000669_2	CDS

The following three_prime_UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Type
Bhi05M000669.utr3p1	Bhi05M000669.utr3p1	three_prime_UTR

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Type
Bhi05M000669	Bhi05M000669-protein	polypeptide

GO Annotation

GO Assignments

This mRNA is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding