Bhi03M000519 (mRNA) Wax gourd (B227) v1

BLAST of Bhi03M000519 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 443.4 bits (1139), Expect = 2.7e-124
Identity = 234/531 (44.07%), Postives = 345/531 (64.97%), Query Frame = 0

Query: 17  TVLMGLSILVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPLIRHLPLFGGPEP-P 76
           T L  L + + + +F  + K+        K  +  K P   G WP+I HL L GG E   
Sbjct: 3   TSLFSLFVPILVFVFIALFKK-------SKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLL 62

Query: 77  HLKLSALADKYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPRPKLLSGKLMGYNY 136
           +  L  +AD YGP  S+QLG++ A V++++E AK CFT +D A+  RP   + K MGYN+
Sbjct: 63  YRTLGKMADHYGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNF 122

Query: 137 AMFIFSPYYNSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKELYQL----GG--- 196
           A+F F+P Y+++WR+MRKI T++LLSNRRL++L+ VR SE+   +K+LY L    GG   
Sbjct: 123 AVFGFAP-YSAFWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLWFKNGGTKP 182

Query: 197 -RVEMKQWFGDLIMNLVLLIVGGKRYFGAGAMV---EEEEAQRCQKAMEESLRLLGMYVL 256
             V++K W  D+ +N+++ +V GKRYFG G  V   + EEA +C+KA+ +   L+G++ +
Sbjct: 183 VMVDLKSWLEDMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTV 242

Query: 257 GDAIPYLGWLDVGGHVKAMKRTFKVLDGMLTQWLEEHKKNR----AGKNNGDFMDVMLSV 316
            DA P L + D+ GH K MK+T   LD +L +W+E H++ R      +N+ DF+DVM+S+
Sbjct: 243 SDAFPTLSFFDLQGHEKEMKQTGSELDVILERWIENHRQQRKFSGTKENDSDFIDVMMSL 302

Query: 317 LEETDNDILHGFHRDTVIKATCLTMVLGASETSIVAVVWALSLLLNNRDTLRKVQEELDT 376
            E+     L  +  +T IK+TCL ++LG S+TS   + WA+SLLLNN++ L+K Q+E+D 
Sbjct: 303 AEQGKLSHLQ-YDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDI 362

Query: 377 HIGRDAKLQESDMEKLVYLEAVVKEAMRLFGAGSLIS-REAAEDIDVDGFHIKKGTWLFI 436
           H+GRD  +++SD+E LVYL+A++KE +RL+ AG L+  REA ED  V G+++  GT L +
Sbjct: 363 HVGRDRNVEDSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIV 422

Query: 437 NLWKIHRDERVWDEPLKFKPERFLTTH-KNFDVKGGQQFELIPFGGGRRICPGLWFGLQL 496
           N+WKI RD +V+ EP +F+PERF+T   K FDV+ GQ FEL+PFG GRR CPG    +Q+
Sbjct: 423 NVWKIQRDPKVYMEPNEFRPERFITGEAKEFDVR-GQNFELMPFGSGRRSCPGSSLAMQV 482

Query: 497 VEFVLGGLLHAFDIWTPEDAAVDMTGTIGMTNMKATPLEVFIKPRLSPMLY 530
           +   L   LH+FD+ T  D  VDM+   G+T  KATPLEV I PR+   L+
Sbjct: 483 LHLGLARFLHSFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRIKEELF 523

BLAST of Bhi03M000519 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 438.7 bits (1127), Expect = 6.6e-123
Identity = 234/530 (44.15%), Postives = 344/530 (64.91%), Query Frame = 0

Query: 17  TVLMGLSILVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPLIRHLPLFGGPEP-P 76
           T L  L + + + +F  + K+        K  ++ K P   G WP+I HL L  G E   
Sbjct: 3   TSLFSLFVPILVFVFIALFKK-------SKKPKHVKAPAPSGAWPIIGHLHLLSGKEQLL 62

Query: 77  HLKLSALADKYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPRPKLLSGKLMGYNY 136
           +  L  +AD+YGP  S++LG+    V++++E AK CFT +D A+  RP   + K MGY+ 
Sbjct: 63  YRTLGKMADQYGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDC 122

Query: 137 AMFIFSPYYNSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKELYQL----GG--- 196
           A+F F+P Y+++WR+MRKI TL+LLSNRRL++L+ VR SE++  +++LY L    GG   
Sbjct: 123 AVFGFAP-YSAFWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLWVKKGGSEP 182

Query: 197 -RVEMKQWFGDLIMNLVLLIVGGKRYFGAGAMVEE--EEAQRCQKAMEESLRLLGMYVLG 256
             V++K W  D+ +N+++ +V GKRYFG G++  E  EEA++C+K +     L+G++ + 
Sbjct: 183 VMVDLKSWLEDMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVS 242

Query: 257 DAIPYLGWLDVGGHVKAMKRTFKVLDGMLTQWLEEHKKNR----AGKNNGDFMDVMLSVL 316
           DA P LGW D  GH K MK+T + LD +L +W+E H++ R       N+ DF+DVMLS+ 
Sbjct: 243 DAFPKLGWFDFQGHEKEMKQTGRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSLA 302

Query: 317 EETDNDILHGFHRDTVIKATCLTMVLGASETSIVAVVWALSLLLNNRDTLRKVQEELDTH 376
           E+     L      T IK+TCL ++LG SETS   + WA+SLLLNN+D L+K Q+E+D H
Sbjct: 303 EQGKFSHLQ-HDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIH 362

Query: 377 IGRDAKLQESDMEKLVYLEAVVKEAMRLFGAGSLIS-REAAEDIDVDGFHIKKGTWLFIN 436
           +GRD  +++SD+E LVY++A++KE +RL+ AG L+  REA ED  V G+++++GT + +N
Sbjct: 363 VGRDRNVEDSDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVN 422

Query: 437 LWKIHRDERVWDEPLKFKPERFLTTH-KNFDVKGGQQFELIPFGGGRRICPGLWFGLQLV 496
           +WKI RD RV+ EP +F+PERF+T   K FDV+ GQ FEL+PFG GRR CPG    +Q++
Sbjct: 423 VWKIQRDPRVYMEPNEFRPERFITGEAKEFDVR-GQNFELMPFGSGRRSCPGSSLAMQVL 482

Query: 497 EFVLGGLLHAFDIWTPEDAAVDMTGTIGMTNMKATPLEVFIKPRLSPMLY 530
              L   L +FD+ T  D  VDMT + G+T  KATPLE+ I PRL   LY
Sbjct: 483 HLGLARFLQSFDVKTVMDMPVDMTESPGLTIPKATPLEILISPRLKEGLY 522

BLAST of Bhi03M000519 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 402.5 bits (1033), Expect = 5.2e-112
Identity = 225/531 (42.37%), Postives = 328/531 (61.77%), Query Frame = 0

Query: 17  TVLMGLSILVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPLIRHLPLFGGPEP-P 76
           T L  L + + + +F  + K+        K  +  K P   G WP+I HL L GG E   
Sbjct: 3   TSLFSLFVSILVFVFIALFKK-------SKKPKYVKAPAPSGAWPIIGHLHLLGGKEQLL 62

Query: 77  HLKLSALADKYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPRPKLLSGKLMGYNY 136
           +  L  +AD YGP  S++LG+    V +++E AK CFT +D A+       + K MGY  
Sbjct: 63  YRTLGKMADHYGPAMSLRLGSSETFVGSSFEVAKDCFTVNDKAL-ASLMTAAAKHMGY-- 122

Query: 137 AMFIFSPYYNSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKELYQL----GG--- 196
                      +W +MRKI  ++LLSNRRL++L  VR SE++  +K+LY L    GG   
Sbjct: 123 ----------VFWLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLWVKKGGSEP 182

Query: 197 -RVEMKQWFGDLIMNLVLLIVGGKRYFGAG---AMVEEEEAQRCQKAMEESLRLLGMYVL 256
             V++K W  D+I N+++ +V GKRYFG G   +    EEA++ +K + +   L+G++ +
Sbjct: 183 VMVDLKSWLEDMIANMIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTV 242

Query: 257 GDAIPYLGWLDVGGHVKAMKRTFKVLDGMLTQWLEEHKKNR----AGKNNGDFMDVMLSV 316
            DA P LGWLD+ GH K MK+T + LD +L +W+E H++ R       N+ DF+DVMLS+
Sbjct: 243 SDAFPKLGWLDLQGHEKEMKQTRRELDVILERWIENHRQQRKVSGTKHNDSDFVDVMLSL 302

Query: 317 LEETDNDILHGFHRDTVIKATCLTMVLGASETSIVAVVWALSLLLNNRDTLRKVQEELDT 376
            E+     L  +  +T IK TCL ++LG SETS   + WA+SLLLNN+D L+KVQ+E+D 
Sbjct: 303 AEQGKLSHLQ-YDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDI 362

Query: 377 HIGRDAKLQESDMEKLVYLEAVVKEAMRLFGAGSLIS-REAAEDIDVDGFHIKKGTWLFI 436
           H+GRD  +++SD++ LVYL+A++KE +RL+ A  L+  REA ED  V G+++  GT L +
Sbjct: 363 HVGRDRNVEDSDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIV 422

Query: 437 NLWKIHRDERVWDEPLKFKPERFLTTH-KNFDVKGGQQFELIPFGGGRRICPGLWFGLQL 496
           N+WKI RD +V+ EP +F+PERF+T   K+FDV+ GQ FEL+PFG GRR CPG    +Q+
Sbjct: 423 NVWKIQRDPKVYMEPNEFRPERFITGEAKDFDVR-GQNFELMPFGSGRRSCPGPSLAMQM 482

Query: 497 VEFVLGGLLHAFDIWTPEDAAVDMTGTIGMTNMKATPLEVFIKPRLSPMLY 530
           +   L   LH+F++ T  D  VDM+ + G+T  KATPLEV I PRL   L+
Sbjct: 483 LHLGLARFLHSFEVKTVLDRPVDMSESPGLTITKATPLEVLINPRLKRELF 511

BLAST of Bhi03M000519 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 394.0 bits (1011), Expect = 1.9e-109
Identity = 225/531 (42.37%), Postives = 318/531 (59.89%), Query Frame = 0

Query: 19  LMGLSILVAIILFRLVMKRVTNNTGGVKGRRNPK-----PPMVGGGWPLIRHLPLFGGPE 78
           L+ L +L A+ +F +++         +K R  PK      PMV G WPL+ HL LF    
Sbjct: 3   LIMLFLLSALFIFPVLIL--------IKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVN 62

Query: 79  PPHLKLSALADKYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPRPKLLSGKLMGY 138
           P H+   A+AD YGP+F  +LG+   ++IN+ E AK+ +T HD  +  RP+L + KL+GY
Sbjct: 63  PTHVTFGAMADVYGPVFMAKLGSIKVMIINSKEVAKEIYTVHD-KLLERPELTASKLLGY 122

Query: 139 NYAMFIFSPYYNSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKELY--------- 198
           N +   FSP Y  YWR++RKI   +L S   + +    R  E + A + LY         
Sbjct: 123 NDSFLTFSP-YGLYWREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKP 182

Query: 199 QLGGRVEMKQWFGDLIMNLVLLIVGGKRYFGAGAMVEEEEAQRCQKAMEESLRLLGMYVL 258
           + G  V+MKQ F DL  N+ L++V GKRYFG     E +EA+RC K + E L    +++L
Sbjct: 183 KEGVLVDMKQEFIDLTANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLL 242

Query: 259 GDAIPYLGWLDVGGHVKAMKRTFKVLDGMLTQWLEEHKKNRA--GKNNGDFMDVMLSVLE 318
            D  P LG+LD     + MKRT K LD +   W+EEHK  R+  G++  D++D+++ +L 
Sbjct: 243 SDVAPVLGFLD-WKTKRGMKRTAKGLDKVAEGWIEEHKNKRSDHGRSENDYLDILIKILG 302

Query: 319 ETDNDILHGFHRDTVIKATCLTMVLGASETSIVAVVWALSLLLNNRDTLRKVQEELDTHI 378
           +     L   H  T IKA CL +VL  SET+IV +VWA+SLLLNN   LRK QEELD+ I
Sbjct: 303 QDKIPGLSDTH--TKIKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKI 362

Query: 379 GRDAKLQESDMEKLVYLEAVVKEAMRLFGAGSLIS-REAAEDIDVD--GFHIKKGTWLFI 438
           G++  ++E D++ LVYL+A+VKE  RL+    L++ R   ED D+     H+  GT L +
Sbjct: 363 GKERVVEELDIKDLVYLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMV 422

Query: 439 NLWKIHRDERVWDEPLKFKPERFLTTHKNFDVKGGQQFELIPFGGGRRICPGLWFGLQLV 498
           + WKIHRD  VW  P +F+PERFLT+++  DV GGQ ++  PFG GRR CP +  G+++V
Sbjct: 423 SAWKIHRDPNVWSNPEQFEPERFLTSNRELDV-GGQSYKFFPFGLGRRSCPAIPLGMRMV 482

Query: 499 EFVLGGLLHAFDIWTPEDAAVDMTGTIGMTNMKATPLEVFIKPRLSPMLYE 531
            ++L   LH+FD+  P    VDMT + G+ N KATPLEV I PRL   LYE
Sbjct: 483 HYLLVRFLHSFDLARPSSQDVDMTESNGLVNHKATPLEVNIIPRLHKSLYE 519

BLAST of Bhi03M000519 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 352.4 bits (903), Expect = 6.2e-97
Identity = 203/512 (39.65%), Postives = 300/512 (58.59%), Query Frame = 0

Query: 26  VAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPLIRHLPLFGGPEPPHLKLSALADK 85
           +A+++F  +  R   +   V     P+P    G  PL  HL L  G +    KL+A++ K
Sbjct: 15  LALVIFGYIFLRKQLSRCEVDSSTIPEPL---GALPLFGHLHLLRGKKLLCKKLAAMSQK 74

Query: 86  YGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPRPKLLSGKLMGYNYAMFIFSPYYN 145
           +GPIFS++LG +  VV ++ +  K CFTT+D+A   RP +  G+ +GYN A    +P Y 
Sbjct: 75  HGPIFSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNNASLTLAP-YG 134

Query: 146 SYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKELYQLGGR---VEMKQWFGDLIMN 205
            YWR++RKI T+ L SN  +++L  +R SEVN  IK LY+  G    V++   F  L  N
Sbjct: 135 DYWRELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLYKGNGGTSIVKIDMLFEFLTFN 194

Query: 206 LVLLIVGGKRYFGAGAMVEEEEAQRCQKAMEESLRLLGMYVLGDAIPYLGWLDVGGHVKA 265
           ++L  + GKR  G G +  +E   R ++A++    L  + ++GD IP+LGWLD   +   
Sbjct: 195 IILRKMVGKR-IGFGEVNSDE--WRYKEALKHCEYLAVIPMIGDVIPWLGWLDFAKN-SQ 254

Query: 266 MKRTFKVLDGMLTQWLEEHKKNRAGKNNGD----FMDVMLSVLEETDNDILHGFHRDTVI 325
           MKR FK LD + T+WL EH K R+ +N  D     MD++L +L E  + ++ G  RD ++
Sbjct: 255 MKRLFKELDSVNTKWLHEHLKKRS-RNEKDQERTIMDLLLDILPE--DIVISGHVRDVIV 314

Query: 326 KATCLTMVLGASETSIVAVVWALSLLLNNRDTLRKVQEELDTHIGRDAKLQESDMEKLVY 385
           KAT L + L  S+++ + + WA+SLLLNN   L   QEE+D  +G+   ++ESD++ L Y
Sbjct: 315 KATILALTLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVGKGRWIEESDIQNLKY 374

Query: 386 LEAVVKEAMRLFGAGSLIS-REAAEDIDVDGFHIKKGTWLFINLWKIHRDERVWDEPLKF 445
           L+A+VKE  RL+    L   REA ED  V G+ ++KGT L +N+WK+HRD ++W +P  F
Sbjct: 375 LQAIVKETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIWKLHRDPKIWPDPKTF 434

Query: 446 KPERFLTTHKNFDVKGGQQFELIPFGGGRRICPGLWFGLQLVEFVLGGLLHAFDIWTPED 505
           KPERF+      +      FE IPFG GRR CPG+  GL++V FVL  LL  F++    D
Sbjct: 435 KPERFMEDKSQCE---KSNFEYIPFGSGRRSCPGVNLGLRVVHFVLARLLQGFELHKVSD 494

Query: 506 AAVDMTGTIGMTNMKATPLEVFIKPRLSPMLY 530
             +DM    G+   K  P+EV + PRL P LY
Sbjct: 495 EPLDMAEGPGLALPKINPVEVVVMPRLDPKLY 512

BLAST of Bhi03M000519 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 6.2e-134
Identity = 256/539 (47.50%), Postives = 353/539 (65.49%), Query Frame = 0

Query: 8   ELTWNNNQTTVLMGLSILVAIILFRL-VMKRVTNNTGGVKGRRNPKPPMVGGGWPLIRHL 67
           EL  +   TT +  +S++  + LF    +K+V  +        + + P VGG WP+  HL
Sbjct: 2   ELVLHFLNTTTIGVVSLIFLLCLFLYGPLKKVHGS--------SKEAPTVGGAWPIFGHL 61

Query: 68  PLFGGPEPPHLKLSALADKYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPRPKLL 127
           PL  G + PH  L ALA+K+GP+F+I+LG   A+V+++WE A++CFTT+D+AV  RPKLL
Sbjct: 62  PLLIGSKSPHKALGALAEKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLL 121

Query: 128 SGKLMGYNYAMFIFSPYYNSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKELYQL 187
             +LM YN AM + +P Y  YWR++RKI   ++LS+ R++ L+ VR SEV  +I ELY +
Sbjct: 122 VAELMCYNNAMLLVAP-YGPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDV 181

Query: 188 G---------GRVEMKQWFGDLIMNLVLLIVGGKRYFGAGAMVEEEEAQRCQKAMEESLR 247
                       VE+KQWF   I N+VL +V GKR+  A A   +E+A++C KA++E +R
Sbjct: 182 WRSQKNESDYASVELKQWFAQPIFNMVLRMVVGKRFLSATA--TDEKAEKCVKAVDEFMR 241

Query: 248 LLGMYVLGDAIPYLGWLDVGGHVKAMKRTFKVLDGMLTQWLEEHKKNRA---GKNNG-DF 307
           L G++ +GDAIPYL WLD GG+ KAMK T K LD M+++WLEEH++ RA   G +   DF
Sbjct: 242 LAGVFTVGDAIPYLRWLDFGGYEKAMKETAKELDVMISEWLEEHRQKRALGEGVDGAQDF 301

Query: 308 MDVMLSVLEETDNDILHGFHRDTVIKATCLTMVLGASETSIVAVVWALSLLLNNRDTLRK 367
           M+VMLS L   D   + G   DT+IK+T LT++   +E SI  ++WA+ L+L N   L  
Sbjct: 302 MNVMLSSL---DGKTIDGIDADTLIKSTVLTIIQAGTEASISTIIWAMCLILKNPLILEN 361

Query: 368 VQEELDTHIGRDAKLQESDMEKLVYLEAVVKEAMRLFGAGSLIS-REAAEDIDVDGFHIK 427
            + ELD  +G+D  + ESD+  LVYL+AVVKE +RL+  G L S RE AED  + G+H+K
Sbjct: 362 -KAELDIQVGKDRCICESDISNLVYLQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVK 421

Query: 428 KGTWLFINLWKIHRDERVWDEPLKFKPERFLTTHKNFDVKGGQQFELIPFGGGRRICPGL 487
           KGT L  N+WKIH D  VW +P +FKP+RFLTTHK+ DVK G  F+L+PFG GRR+CPG+
Sbjct: 422 KGTRLITNIWKIHTDPNVWSDPFEFKPDRFLTTHKDIDVK-GHHFQLLPFGSGRRVCPGI 481

Query: 488 WFGLQLVEFVLGGLLHAFDIWTPEDAAVDMTGTIGMTNMKATPLEVFIKPRLSPMLYES 532
            FGLQ V   L   LH+F+I  P    +DMT   G+TN KATPLEV +KP LSP  Y+S
Sbjct: 482 SFGLQTVHLALASFLHSFEILNPSTEPLDMTEAFGVTNTKATPLEVLVKPCLSPSCYKS 524

BLAST of Bhi03M000519 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 7.6e-132
Identity = 241/501 (48.10%), Postives = 328/501 (65.47%), Query Frame = 0

Query: 46  KGRRNPKPPMVGGGWPLIRHLPLFGGPEPPHLKLSALADKYGPIFSIQLGTHSAVVINNW 105
           K  R    P+V G WP++ HL L  G + PH  L ALADKYGP+F+I+LG   A+V++NW
Sbjct: 31  KNSRGKDAPVVSGAWPILGHLSLLNGSQTPHKVLGALADKYGPLFTIKLGMKPALVLSNW 90

Query: 106 EAAKQCFTTHDIAVFPRPKLLSGKLMGYNYAMFIFSPYYNSYWRKMRKITTLDLLSNRRL 165
           E +K+ FTT+D+AV  RPKL++ ++M YN A    +P Y  YWR++RKI T + LSNRR+
Sbjct: 91  EMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFVGLAP-YGPYWRELRKIVTFEFLSNRRI 150

Query: 166 KLLRQVRESEVNRAIKELYQLGGR----------VEMKQWFGDLIMNLVLLIVGGKRYFG 225
           +    +R SEV  +IKEL+ +             V++KQW   L  N+V+ +V GKRYFG
Sbjct: 151 EQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTLVDIKQWLAYLTFNMVVRMVVGKRYFG 210

Query: 226 AGAMVEEEEAQRCQKAMEESLRLLGMYVLGDAIPYLGWLDVGGHVKAMKRTFKVLDGMLT 285
              +  +++AQR  K + E + L+G + + D +P L WLD+GGH KAMK   K +D +L+
Sbjct: 211 VMHVEGKDKAQRFMKNIREFMNLMGTFTVADGVPCLRWLDLGGHEKAMKANAKEVDKLLS 270

Query: 286 QWLEEHK-KNRAGKN---NGDFMDVMLSVLEETDNDILHGFHRDTVIKATCLTMVLGASE 345
           +WLEEH+ K   G+N   + DFMDVM+S L       +  F  DT+ KAT L ++LG ++
Sbjct: 271 EWLEEHRQKKLLGENVESDRDFMDVMISALNGAQ---IGAFDADTICKATSLELILGGTD 330

Query: 346 TSIVAVVWALSLLLNNRDTLRKVQEELDTHIGRDAKLQESDMEKLVYLEAVVKEAMRLFG 405
           ++ V + WALSLLL N   L K +EE+D  IG+D  ++ESD+ KLVYL+A+VKE +RL+ 
Sbjct: 331 STAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDEYIRESDISKLVYLQAIVKETLRLYP 390

Query: 406 AGSLIS-REAAEDIDVDGFHIKKGTWLFINLWKIHRDERVWDEPLKFKPERFLTTHKNFD 465
                S RE  E+  + G+HIKKGT L  NLWKIHRD  VW +PL+FKPERFLTTHK+ D
Sbjct: 391 PAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIHRDPSVWSDPLEFKPERFLTTHKDVD 450

Query: 466 VKGGQQFELIPFGGGRRICPGLWFGLQLVEFVLGGLLHAFDIWTPEDAAVDMTGTIGMTN 525
           ++ G  FEL+PFG GRR+C G+  GL +V F L  LLH+FDI  P    VDMT   G TN
Sbjct: 451 LR-GHNFELLPFGSGRRVCAGMSLGLNMVHFTLANLLHSFDILNPSAEPVDMTEFFGFTN 510

Query: 526 MKATPLEVFIKPRLSPMLYES 532
            KATPLE+ +KPR SP  YE+
Sbjct: 511 TKATPLEILVKPRQSPNYYET 526

BLAST of Bhi03M000519 vs. ExPASy Swiss-Prot
Match: O81972 (Cytochrome P450 82A2 OS=Glycine max OX=3847 GN=CYP82A2 PE=2 SV=1)

HSP 1 Score: 466.1 bits (1198), Expect = 5.4e-130
Identity = 251/530 (47.36%), Postives = 336/530 (63.40%), Query Frame = 0

Query: 14  NQTTVLMGLSILVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPLIRHLPLFGGPE 73
           N TT+  G+ ++  I+L  L ++      GG        PP V G WP+I HLPL  G +
Sbjct: 6   NSTTI--GVGVVSLILLLYLFLR------GGSWKSGEEGPPTVAGAWPIIGHLPLLLGSK 65

Query: 74  PPHLKLSALADKYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPRPKLLSGKLMGY 133
            PH  L  LADKYGPIFSI++G  +AVV++NWE AK+C+TT+DIAV   P L+S  L+ Y
Sbjct: 66  TPHKTLGDLADKYGPIFSIKIGAKNAVVVSNWEMAKECYTTNDIAVSSLPDLISANLLCY 125

Query: 134 NYAMFIFSPYYNSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKELY--------- 193
           N +M + +P Y  YWR++RKI   + LS  R++ L  VR SEV  +I EL+         
Sbjct: 126 NRSMIVVAP-YGPYWRQLRKILMSEFLSPSRVEQLHHVRVSEVQSSITELFRDWRSNKNV 185

Query: 194 QLG-GRVEMKQWFGDLIMNLVLLIVGGKRYFGAGAMVEEEEAQRCQKAMEESLRLLGMYV 253
           Q G   VE+KQWF  L+ N++L +V GKRYF A    ++E+A RC KA++E +RL   + 
Sbjct: 186 QSGFATVELKQWFSLLVFNMILRMVCGKRYFSASTS-DDEKANRCVKAVDEFVRLAATFT 245

Query: 254 LGDAIPYLGWLDVGGHVKAMKRTFKVLDGMLTQWLEEHK-KNRAGKNNGDFMDVMLSVLE 313
           +GDAIPYL W D GG+   M+ T K LD ++ +WL+EH+ K + G+N  D M V+LS+LE
Sbjct: 246 VGDAIPYLRWFDFGGYENDMRETGKELDEIIGEWLDEHRQKRKMGENVQDLMSVLLSLLE 305

Query: 314 ETDNDILHGFHRDTVIKATCLTMVLGASETSIVAVVWALSLLLNNRDTLRKVQEELDTHI 373
                 + G + D VIK+  LT++   +E SI  ++WA SL+LNN   L K++ ELD  +
Sbjct: 306 ---GKTIEGMNVDIVIKSFVLTVIQAGTEASITTLIWATSLILNNPSVLEKLKAELDIQV 365

Query: 374 GRDAKLQESDMEKLVYLEAVVKEAMRLFGAGSLI-SREAAEDIDVDGFHIKKGTWLFINL 433
           G++  + ESD+ KL YL+AVVKE +RL+    L   RE  ED  + G+ +KKGT L  NL
Sbjct: 366 GKERYICESDLSKLTYLQAVVKETLRLYPPAPLSRPREFEEDCTIGGYTVKKGTRLITNL 425

Query: 434 WKIHRDERVWDEPLKFKPERFLTTHKNFDVKGGQQFELIPFGGGRRICPGLWFGLQLVEF 493
            KIH D  VW  PL+FKPERFLTT K+ D+K GQ F+L+PFGGGRRICPG+  GLQ V  
Sbjct: 426 SKIHTDHNVWSNPLEFKPERFLTTDKDIDMK-GQHFQLLPFGGGRRICPGINLGLQTVRL 485

Query: 494 VLGGLLHAFDIWTPEDAAVDMTGTIGMTNMKATPLEVFIKPRLSPMLYES 532
            L   LH+F+I  P    +DMT     TN KATPLE+ IKPRLSP  YES
Sbjct: 486 TLASFLHSFEILNPSTEPLDMTEVFRATNTKATPLEILIKPRLSPSCYES 521

BLAST of Bhi03M000519 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 464.9 bits (1195), Expect = 1.2e-129
Identity = 243/554 (43.86%), Postives = 346/554 (62.45%), Query Frame = 0

Query: 13  NNQTTVLMGLSILVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPLIRHLPLFGGP 72
           N   T  +    L++++ F     +V++           +PP++ G WPL+ HLPL    
Sbjct: 4   NYLNTTTIAFISLISLLFFLFRFSKVSH---------TKEPPIISGSWPLLGHLPLMRNT 63

Query: 73  EPPHLKLSALADKYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPRPKLLSGKLMG 132
           + PH  L AL DKYGPIF+I+LG  +A+V++NWE AK+CFT +DI V  RPK ++ +LM 
Sbjct: 64  QTPHKTLGALVDKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMS 123

Query: 133 YNYAMFIFSPYYNSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKELYQLGGR--- 192
           YN A   ++P Y +YWR++RKI TL++LSNRR++LL  +R SEV  +IKEL  +      
Sbjct: 124 YNQAFIGWAP-YGAYWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQIS 183

Query: 193 -------------------------VEMKQWFGDLIMNLVLLIVGGKRYFGAGAMVEEEE 252
                                    VE+K+WF  L +N+VL +V GKR FG   +  +EE
Sbjct: 184 SQYGLLDDTKSSSTNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEE 243

Query: 253 AQRCQKAMEESLRLLGMYVLGDAIPYLGWLDVGGHVKAMKRTFKVLDGMLTQWLEEHKKN 312
           A+R  + + + +RL+G + +GD +P+L WLD+GGH K MK+  K  D ML +WLEEH++ 
Sbjct: 244 AKRFLENIRDFMRLIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREK 303

Query: 313 RA-GKNN-----GDFMDVMLSVLEETDNDILHGFHRDTVIKATCLTMVLGASETSIVAVV 372
           +  G  +      DFMD ML VL++     + GF  DT+IKAT L ++LG S+T+   + 
Sbjct: 304 KGLGSEDKVVGERDFMDAMLLVLKDKP---IEGFDVDTIIKATTLELILGGSDTTAGTLT 363

Query: 373 WALSLLLNNRDTLRKVQEELDTHIGRDAKLQESDMEKLVYLEAVVKEAMRLFGAGSLIS- 432
           WA+ LLL +   L K++EEL+T+IG++  + ESD+ KLVYL A++KE +RL+      S 
Sbjct: 364 WAMCLLLKHPHVLEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSP 423

Query: 433 REAAEDIDVDGFHIKKGTWLFINLWKIHRDERVWDEPLKFKPERFLTTHKNFDVKGGQQF 492
           RE  ED  + G+HIKKGT L  NLWKIHRD  VW +PL+FKPERFL+THK+ DV+ GQ F
Sbjct: 424 REFTEDCTIGGYHIKKGTRLMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVR-GQNF 483

Query: 493 ELIPFGGGRRICPGLWFGLQLVEFVLGGLLHAFDIWTPEDAAVDMTGTIGMTNMKATPLE 532
           EL+PFG GRR+C G+  GL +V ++L   LH+F+I  P   ++D+T  +     KATPLE
Sbjct: 484 ELLPFGSGRRMCAGMSLGLHMVHYILANFLHSFEILNPSPESIDVTEVLEFVTTKATPLE 543

BLAST of Bhi03M000519 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 1.9e-127
Identity = 244/529 (46.12%), Postives = 335/529 (63.33%), Query Frame = 0

Query: 17  TVLMGLSILVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPLIRHLPLFGGPEPPH 76
           T+L+G  +L+    F  V K+  N        +  +PP   G WP+I HL L    + PH
Sbjct: 9   TLLLGFFVLLP-CFFYFVWKKPNN--------KIKEPPQPAGAWPIIGHLHLLARGDLPH 68

Query: 77  LKLSALADKYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPRPKLLSGKLMGYNYA 136
             LS+ ADK GP+F IQLG H A+V+NN E AK+CFTT+D     RP  ++ K+MGYNY 
Sbjct: 69  KILSSFADKNGPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYV 128

Query: 137 MFIFSPYYNSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKELYQLGGR------- 196
           M   +P Y  YWR MRKI  L+ LSNRRL+ L+ V  SE++ + KELY+L          
Sbjct: 129 MLGVAP-YGPYWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLWETQNIDFCL 188

Query: 197 VEMKQWFGDLIMNLVLLIVGGKRYFG--AGAMVEEEEAQRCQKAMEESLRLLGMYVLGDA 256
           V+MKQW  DL +N+ + +V GKR+FG  + +  EE E+  C K +    RL+G +VL D 
Sbjct: 189 VDMKQWLADLTLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDY 248

Query: 257 IPYLGWLDVGGHVKAMKRTFKVLDGMLTQWLEEHKKNR--AGK--NNGDFMDVMLSVLEE 316
           +PYL WLD+GGH K MKRT K LD +   WL+EHK+ R   GK  ++ DFMDVMLS+LEE
Sbjct: 249 LPYLRWLDLGGHEKEMKRTVKELDILFKGWLDEHKRKRLSGGKEDDDQDFMDVMLSILEE 308

Query: 317 T--DNDILHGFHRDTVIKATCLTMVLGASETSIVAVVWALSLLLNNRDTLRKVQEELDTH 376
           +   ND+      DT+ K  CL ++LG ++T+   + WALSLLLNN + L+K Q+ELD H
Sbjct: 309 SKLGNDV------DTINKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLH 368

Query: 377 IGRDAKLQESDMEKLVYLEAVVKEAMRLFGAGSLIS-REAAEDIDVDGFHIKKGTWLFIN 436
           +GRD  + ESD+ KL Y++A++KE +RL+  G L+  R   ED  + G+H++ GT L +N
Sbjct: 369 VGRDRNVDESDLVKLTYIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVN 428

Query: 437 LWKIHRDERVWDEPLKFKPERFLTTHKNFDVKGGQQFELIPFGGGRRICPGLWFGLQLVE 496
            WKI RD  VW +P +++PERFL   ++ D+K GQ FELIPFG GRR CP +   LQ++ 
Sbjct: 429 AWKIQRDPLVWSQPHEYQPERFL--ERDVDMK-GQHFELIPFGSGRRACPAISLALQVLP 488

Query: 497 FVLGGLLHAFDIWTPEDAAVDMTGTIGMTNMKATPLEVFIKPRLSPMLY 530
             L  +LH F++ TP    VDMT T G+ + KATPLEV + PR+SP  +
Sbjct: 489 LTLAHILHGFELRTPNQNKVDMTETPGIVHAKATPLEVLVAPRISPKCF 518

BLAST of Bhi03M000519 vs. NCBI nr
Match: XP_038883369.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 535/535 (100.00%), Postives = 535/535 (100.00%), Query Frame = 0

Query: 1   MEVEAMAELTWNNNQTTVLMGLSILVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGW 60
           MEVEAMAELTWNNNQTTVLMGLSILVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGW
Sbjct: 1   MEVEAMAELTWNNNQTTVLMGLSILVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGW 60

Query: 61  PLIRHLPLFGGPEPPHLKLSALADKYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVF 120
           PLIRHLPLFGGPEPPHLKLSALADKYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVF
Sbjct: 61  PLIRHLPLFGGPEPPHLKLSALADKYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVF 120

Query: 121 PRPKLLSGKLMGYNYAMFIFSPYYNSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAI 180
           PRPKLLSGKLMGYNYAMFIFSPYYNSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAI
Sbjct: 121 PRPKLLSGKLMGYNYAMFIFSPYYNSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAI 180

Query: 181 KELYQLGGRVEMKQWFGDLIMNLVLLIVGGKRYFGAGAMVEEEEAQRCQKAMEESLRLLG 240
           KELYQLGGRVEMKQWFGDLIMNLVLLIVGGKRYFGAGAMVEEEEAQRCQKAMEESLRLLG
Sbjct: 181 KELYQLGGRVEMKQWFGDLIMNLVLLIVGGKRYFGAGAMVEEEEAQRCQKAMEESLRLLG 240

Query: 241 MYVLGDAIPYLGWLDVGGHVKAMKRTFKVLDGMLTQWLEEHKKNRAGKNNGDFMDVMLSV 300
           MYVLGDAIPYLGWLDVGGHVKAMKRTFKVLDGMLTQWLEEHKKNRAGKNNGDFMDVMLSV
Sbjct: 241 MYVLGDAIPYLGWLDVGGHVKAMKRTFKVLDGMLTQWLEEHKKNRAGKNNGDFMDVMLSV 300

Query: 301 LEETDNDILHGFHRDTVIKATCLTMVLGASETSIVAVVWALSLLLNNRDTLRKVQEELDT 360
           LEETDNDILHGFHRDTVIKATCLTMVLGASETSIVAVVWALSLLLNNRDTLRKVQEELDT
Sbjct: 301 LEETDNDILHGFHRDTVIKATCLTMVLGASETSIVAVVWALSLLLNNRDTLRKVQEELDT 360

Query: 361 HIGRDAKLQESDMEKLVYLEAVVKEAMRLFGAGSLISREAAEDIDVDGFHIKKGTWLFIN 420
           HIGRDAKLQESDMEKLVYLEAVVKEAMRLFGAGSLISREAAEDIDVDGFHIKKGTWLFIN
Sbjct: 361 HIGRDAKLQESDMEKLVYLEAVVKEAMRLFGAGSLISREAAEDIDVDGFHIKKGTWLFIN 420

Query: 421 LWKIHRDERVWDEPLKFKPERFLTTHKNFDVKGGQQFELIPFGGGRRICPGLWFGLQLVE 480
           LWKIHRDERVWDEPLKFKPERFLTTHKNFDVKGGQQFELIPFGGGRRICPGLWFGLQLVE
Sbjct: 421 LWKIHRDERVWDEPLKFKPERFLTTHKNFDVKGGQQFELIPFGGGRRICPGLWFGLQLVE 480

Query: 481 FVLGGLLHAFDIWTPEDAAVDMTGTIGMTNMKATPLEVFIKPRLSPMLYESTSPY 536
           FVLGGLLHAFDIWTPEDAAVDMTGTIGMTNMKATPLEVFIKPRLSPMLYESTSPY
Sbjct: 481 FVLGGLLHAFDIWTPEDAAVDMTGTIGMTNMKATPLEVFIKPRLSPMLYESTSPY 535

BLAST of Bhi03M000519 vs. NCBI nr
Match: XP_022133138.1 (cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 655.6 bits (1690), Expect = 3.7e-184
Identity = 339/507 (66.86%), Postives = 392/507 (77.32%), Query Frame = 0

Query: 3   VEAMAELTWNNNQTTVLMGLSILVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPL 62
           +E   +  W N   T+L G   ++AI   R + K  T         +  KPP V GGWPL
Sbjct: 1   MEPSPQFHWLNLIATLLGG---VIAINFLRFLHKTFT----AAAASKGLKPPSVPGGWPL 60

Query: 63  IRHLPLFGGPEPPHLKLSALADKYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPR 122
           I HLPL GGP+ PH+KL ALAD+YGPIFSIQLG H AVVIN+WEAAKQCFTTHD+AV  R
Sbjct: 61  IGHLPLLGGPQLPHVKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFR 120

Query: 123 PKLLSGKLMGYNYAMFIFSPYYNSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKE 182
           PK+L+ +++GYNYA+F F P Y+SYWRKMRK TTL LLS+RRL+LLR VRESEVN+AIKE
Sbjct: 121 PKILAAQILGYNYAVFAFGP-YDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKE 180

Query: 183 LYQL---------GGRVEMKQWFGDLIMNLVLLIVGGKRYFGAGAMVEEEEAQRCQKAME 242
           LYQ+          G VEMKQW GDL MNL+L +V GKRYFG  AMVEE EA+ CQKAM+
Sbjct: 181 LYQIWKERRDKQGQGLVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMK 240

Query: 243 ESLRLLGMYVLGDAIPYLGWLDVGGHVKAMKRTFKVLDGMLTQWLEEHKKNRA---GKNN 302
           E LRL+G+++LGDA+PYLGWLDVGGHVKAMKRT K LD +LTQWLEEHKK RA    K +
Sbjct: 241 EFLRLMGLFILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKED 300

Query: 303 GDFMDVMLSVLEET-DN----DILHGFHRDTVIKATCLTMVLGASETSIVAVVWALSLLL 362
            DFMDVMLS LEET DN    D +H F  DT+IKATCLTMVLG S+T+ V V WALSLLL
Sbjct: 301 KDFMDVMLSTLEETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLL 360

Query: 363 NNRDTLRKVQEELDTHIGRDAKLQESDMEKLVYLEAVVKEAMRLFGAGSLISREAAEDID 422
           NN   L KVQEE+D HIGRD +L+ESDM +LVYL+AVVKE MRL+GAG LI RE +ED  
Sbjct: 361 NNHPALIKVQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLILRETSEDFA 420

Query: 423 VDGFHIKKGTWLFINLWKIHRDERVWDEPLKFKPERFLTTHKNFDVKGGQQFELIPFGGG 482
           +DGFHI+KGT LFIN WKIHRD RVW EPL+FKPERFLTTH++FDV  GQQFELIPFGGG
Sbjct: 421 IDGFHIEKGTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDV-NGQQFELIPFGGG 480

Query: 483 RRICPGLWFGLQLVEFVLGGLLHAFDI 493
           RR CPGL FGLQ+V+ +LG LLHAFDI
Sbjct: 481 RRACPGLSFGLQMVQLILGALLHAFDI 498

BLAST of Bhi03M000519 vs. NCBI nr
Match: XP_022132489.1 (cytochrome P450 CYP82D47-like [Momordica charantia] >XP_022132490.1 cytochrome P450 CYP82D47-like [Momordica charantia])

HSP 1 Score: 605.1 bits (1559), Expect = 5.7e-169
Identity = 312/477 (65.41%), Postives = 363/477 (76.10%), Query Frame = 0

Query: 3   VEAMAELTWNNNQTTVLMGLSILVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPL 62
           +E  A+  W +  TT+L G   ++AI  FR ++K  T + G        KPP V GGWPL
Sbjct: 1   MEPNAQFNWLSLITTLLAG---VIAINFFRFLLKTFTASKG-------LKPPTVAGGWPL 60

Query: 63  IRHLPLFGGPEPPHLKLSALADKYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPR 122
           I HLPL  GPE PH+KLSALA++YGPIFSIQLG   AVVIN+WEAAKQCFTTHD+A+  R
Sbjct: 61  IGHLPLLAGPELPHIKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSR 120

Query: 123 PKLLSGKLMGYNYAMFIFSPYYNSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKE 182
           P +L+ K++GYNYAMF F P Y+SYWRKMRK T L LLSNRRL+LLR VRESEVN+AIKE
Sbjct: 121 PNILASKILGYNYAMFAFGP-YDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKE 180

Query: 183 LY---------QLGGRVEMKQWFGDLIMNLVLLIVGGKRYFGAGAMVEEEEAQRCQKAME 242
           LY         Q    VEMKQW GDL MNL+L +V GKRYFGA AMV E EA+ C +A +
Sbjct: 181 LYTAWKERRDEQGQVLVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATK 240

Query: 243 ESLRLLGMYVLGDAIPYLGWLDVGGHVKAMKRTFKVLDGMLTQWLEEHKKNRA---GKNN 302
           E LRL+G++ LGD +PYLGWLDVGGHVKAMKRT K LD MLT+WLEEH+K+RA    K +
Sbjct: 241 EFLRLMGLFTLGDVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKED 300

Query: 303 GDFMDVMLSVLEETD----NDILHGFHRDTVIKATCLTMVLGASETSIVAVVWALSLLLN 362
            DFMDVMLSVLEE      +D  H F  DT+IKATCLTMVLG S+T++V V WALSLLLN
Sbjct: 301 KDFMDVMLSVLEEETANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLN 360

Query: 363 NRDTLRKVQEELDTHIGRDAKLQESDMEKLVYLEAVVKEAMRLFGAGSLISREAAEDIDV 422
           NR  LRKVQ+ELD  IGRD +L ESD+ KLVY +AVVKE MRL+G   L  RE +ED  +
Sbjct: 361 NRPALRKVQQELDARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLFVRETSEDFAI 420

Query: 423 DGFHIKKGTWLFINLWKIHRDERVWDEPLKFKPERFLTTHKNFDVKGGQQFELIPFG 464
           DGFHI+KGTWLF+NLWKIHRDERVW EPL+FKPERFLTTHK+FDVK GQQFELIPFG
Sbjct: 421 DGFHIEKGTWLFMNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVK-GQQFELIPFG 465

BLAST of Bhi03M000519 vs. NCBI nr
Match: XP_034199033.1 (cytochrome P450 CYP82D47-like [Prunus dulcis] >VVA11544.1 PREDICTED: cytochrome P450 [Prunus dulcis])

HSP 1 Score: 586.3 bits (1510), Expect = 2.7e-163
Identity = 294/516 (56.98%), Postives = 378/516 (73.26%), Query Frame = 0

Query: 25  LVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPLIRHLPLFGGPEPPHLKLSALAD 84
           ++AI+LF   + + +++    KG   PK P V GGWPL+ HL LF G + PH+ L++L D
Sbjct: 26  ILAILLFSYFIIKRSSSAAKAKG---PKLPKVAGGWPLLGHLGLFRGSQLPHIALASLVD 85

Query: 85  KYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPRPKLLSGKLMGYNYAMFIFSPYY 144
           KYGP F+I +G HSA+VI+ WEAAK CFTT+DI V  RP  L  K +GYN+AMF FSP Y
Sbjct: 86  KYGPAFTINIGIHSALVISTWEAAKDCFTTNDIVVSSRPATLGAKHLGYNFAMFGFSP-Y 145

Query: 145 NSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKELYQL--------GGR-VEMKQW 204
             YWR+MRK+T+L+LLSNRRL+LL++VR SEV  ++KELY L        G R VEMKQW
Sbjct: 146 GPYWREMRKLTSLELLSNRRLELLKKVRVSEVEMSLKELYALWIKRKESSGERLVEMKQW 205

Query: 205 FGDLIMNLVLLIVGGKRYFGAGAMVEEEEAQRCQKAMEESLRLLGMYVLGDAIPYLGWLD 264
           FGDL +N++  +V GKR F  G + EE+EA+R QKAM E   L+G++VLGDA+P+L WLD
Sbjct: 206 FGDLTLNVIFRMVAGKRCFMNGNLSEEKEARRWQKAMREFFHLVGLFVLGDAVPWLSWLD 265

Query: 265 VGGHVKAMKRTFKVLDGMLTQWLEEHKKNRAGKNNGDFMDVMLSVLEETDNDILHGFHRD 324
           +GG  KAMK+T K LDG++ +WLEEHK+ R    + DFMDVMLS ++ TD   + GF  D
Sbjct: 266 LGGQQKAMKKTAKELDGIVAEWLEEHKQKRTKGKDQDFMDVMLSAIDGTD---VAGFDAD 325

Query: 325 TVIKATCLTMVLGASETSIVAVVWALSLLLNNRDTLRKVQEELDTHIGRDAKLQESDMEK 384
           TVIKATCL+++ G S+T++V + W LSLLLNNR  L+KV EELD ++G+   L ESD+  
Sbjct: 326 TVIKATCLSLIAGGSDTTMVTLTWTLSLLLNNRQVLKKVYEELDQYVGKGRLLDESDINN 385

Query: 385 LVYLEAVVKEAMRLFGAGSLI-SREAAEDIDVDGFHIKKGTWLFINLWKIHRDERVWDEP 444
           LVYL+A VKEAMRL  AG L   RE  ED  V G+H+ KGTWL +NLWKI  D RVWD+P
Sbjct: 386 LVYLQATVKEAMRLCPAGPLSGQREFTEDCTVGGYHVPKGTWLLVNLWKIQTDPRVWDDP 445

Query: 445 LKFKPERFLTTHKNFDVKGGQQFELIPFGGGRRICPGLWFGLQLVEFVLGGLLHAFDIWT 504
           ++FKPERFLTTHK+ DV+ GQQFEL+PFG GRR CPG+ FGLQ+    L   LH+FD+ T
Sbjct: 446 MEFKPERFLTTHKDVDVR-GQQFELMPFGSGRRACPGISFGLQMTLLTLASFLHSFDVTT 505

Query: 505 PEDAAVDMTGTIGMTNMKATPLEVFIKPRLSPMLYE 531
            E+  VDMTG+IG+TN+K TPL+V +KPRLSP LY+
Sbjct: 506 QENTPVDMTGSIGLTNIKLTPLDVLVKPRLSPNLYD 533

BLAST of Bhi03M000519 vs. NCBI nr
Match: XP_007226598.2 (cytochrome P450 CYP82D47 [Prunus persica] >ONI32809.1 hypothetical protein PRUPE_1G387100 [Prunus persica])

HSP 1 Score: 582.8 bits (1501), Expect = 3.0e-162
Identity = 294/516 (56.98%), Postives = 376/516 (72.87%), Query Frame = 0

Query: 25  LVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPLIRHLPLFGGPEPPHLKLSALAD 84
           ++AI+LF   + + +++    KG   PK P V GGWPL+ H  LF G + PH+ L++L D
Sbjct: 26  ILAILLFSYFIIKRSSSAAKAKG---PKLPKVAGGWPLLGHFGLFRGSQLPHIALASLVD 85

Query: 85  KYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPRPKLLSGKLMGYNYAMFIFSPYY 144
           KYGP F+I +G HSA+VI+ WEAAK CFTT+DI V  RP  L  K +GYN+AMF FSP Y
Sbjct: 86  KYGPAFTINIGIHSALVISTWEAAKDCFTTNDIVVSSRPATLGAKHLGYNFAMFGFSP-Y 145

Query: 145 NSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKELYQL--------GGR-VEMKQW 204
            SYWR+MRK+T+L+LLSNRRL+LL++VR SEV  ++KELY L        G R VEMKQW
Sbjct: 146 GSYWREMRKLTSLELLSNRRLELLKKVRVSEVEMSLKELYTLWIKRKESSGERLVEMKQW 205

Query: 205 FGDLIMNLVLLIVGGKRYFGAGAMVEEEEAQRCQKAMEESLRLLGMYVLGDAIPYLGWLD 264
           FGDL +N++  +V GKR F  G + EE+EA+R QKAM E   L G++VLGDA+P+L WLD
Sbjct: 206 FGDLTLNVIFRMVAGKRCFMNGNLSEEKEARRWQKAMREFFHLAGLFVLGDAVPWLSWLD 265

Query: 265 VGGHVKAMKRTFKVLDGMLTQWLEEHKKNRAGKNNGDFMDVMLSVLEETDNDILHGFHRD 324
           +GG  KAMK+T K LD ++ +WLEEHK+ R    + DFMDVMLS ++ TD   + GF  D
Sbjct: 266 LGGQQKAMKKTAKELDSIVAEWLEEHKQKRTKGKDQDFMDVMLSAIDGTD---VAGFDAD 325

Query: 325 TVIKATCLTMVLGASETSIVAVVWALSLLLNNRDTLRKVQEELDTHIGRDAKLQESDMEK 384
           TVIKATCL ++ GAS+T++V + W LSLLLNNR  L+KV EELD ++G+   L ESD+  
Sbjct: 326 TVIKATCLILIAGASDTTMVTLTWTLSLLLNNRQVLKKVYEELDQYVGKGRLLDESDINN 385

Query: 385 LVYLEAVVKEAMRLFGAGSLI-SREAAEDIDVDGFHIKKGTWLFINLWKIHRDERVWDEP 444
           LVYL+A VKEAMRL  AG L   RE  ED  V G+H+ KGTWL +NLWKI  D RVW +P
Sbjct: 386 LVYLQATVKEAMRLCPAGPLSGQREFTEDCTVGGYHVPKGTWLLVNLWKIQTDPRVWADP 445

Query: 445 LKFKPERFLTTHKNFDVKGGQQFELIPFGGGRRICPGLWFGLQLVEFVLGGLLHAFDIWT 504
           ++FKPERFLTTHK+ DV+ GQQFEL+PFG GRR CPG+ FGLQ+    L   LH+FD+ T
Sbjct: 446 MEFKPERFLTTHKDVDVR-GQQFELMPFGSGRRACPGISFGLQMTLLTLASFLHSFDVTT 505

Query: 505 PEDAAVDMTGTIGMTNMKATPLEVFIKPRLSPMLYE 531
            E+A VDMTG+IG+TN+K TPL+V +KPRLSP LY+
Sbjct: 506 QENAPVDMTGSIGLTNIKLTPLDVLVKPRLSPNLYD 533

BLAST of Bhi03M000519 vs. ExPASy TrEMBL
Match: A0A6J1BY79 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005812 PE=3 SV=1)

HSP 1 Score: 655.6 bits (1690), Expect = 1.8e-184
Identity = 339/507 (66.86%), Postives = 392/507 (77.32%), Query Frame = 0

Query: 3   VEAMAELTWNNNQTTVLMGLSILVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPL 62
           +E   +  W N   T+L G   ++AI   R + K  T         +  KPP V GGWPL
Sbjct: 1   MEPSPQFHWLNLIATLLGG---VIAINFLRFLHKTFT----AAAASKGLKPPSVPGGWPL 60

Query: 63  IRHLPLFGGPEPPHLKLSALADKYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPR 122
           I HLPL GGP+ PH+KL ALAD+YGPIFSIQLG H AVVIN+WEAAKQCFTTHD+AV  R
Sbjct: 61  IGHLPLLGGPQLPHVKLGALADEYGPIFSIQLGIHQAVVINSWEAAKQCFTTHDVAVSFR 120

Query: 123 PKLLSGKLMGYNYAMFIFSPYYNSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKE 182
           PK+L+ +++GYNYA+F F P Y+SYWRKMRK TTL LLS+RRL+LLR VRESEVN+AIKE
Sbjct: 121 PKILAAQILGYNYAVFAFGP-YDSYWRKMRKTTTLHLLSSRRLELLRDVRESEVNQAIKE 180

Query: 183 LYQL---------GGRVEMKQWFGDLIMNLVLLIVGGKRYFGAGAMVEEEEAQRCQKAME 242
           LYQ+          G VEMKQW GDL MNL+L +V GKRYFG  AMVEE EA+ CQKAM+
Sbjct: 181 LYQIWKERRDKQGQGLVEMKQWVGDLTMNLILGVVAGKRYFGPTAMVEEAEARACQKAMK 240

Query: 243 ESLRLLGMYVLGDAIPYLGWLDVGGHVKAMKRTFKVLDGMLTQWLEEHKKNRA---GKNN 302
           E LRL+G+++LGDA+PYLGWLDVGGHVKAMKRT K LD +LTQWLEEHKK RA    K +
Sbjct: 241 EFLRLMGLFILGDAVPYLGWLDVGGHVKAMKRTSKELDHILTQWLEEHKKKRASGKSKED 300

Query: 303 GDFMDVMLSVLEET-DN----DILHGFHRDTVIKATCLTMVLGASETSIVAVVWALSLLL 362
            DFMDVMLS LEET DN    D +H F  DT+IKATCLTMVLG S+T+ V V WALSLLL
Sbjct: 301 KDFMDVMLSTLEETPDNVVASDSIHSFDVDTIIKATCLTMVLGGSDTTTVVVTWALSLLL 360

Query: 363 NNRDTLRKVQEELDTHIGRDAKLQESDMEKLVYLEAVVKEAMRLFGAGSLISREAAEDID 422
           NN   L KVQEE+D HIGRD +L+ESDM +LVYL+AVVKE MRL+GAG LI RE +ED  
Sbjct: 361 NNHPALIKVQEEVDAHIGRDRELKESDMSELVYLQAVVKETMRLYGAGGLILRETSEDFA 420

Query: 423 VDGFHIKKGTWLFINLWKIHRDERVWDEPLKFKPERFLTTHKNFDVKGGQQFELIPFGGG 482
           +DGFHI+KGT LFIN WKIHRD RVW EPL+FKPERFLTTH++FDV  GQQFELIPFGGG
Sbjct: 421 IDGFHIEKGTRLFINQWKIHRDGRVWGEPLEFKPERFLTTHRDFDV-NGQQFELIPFGGG 480

Query: 483 RRICPGLWFGLQLVEFVLGGLLHAFDI 493
           RR CPGL FGLQ+V+ +LG LLHAFDI
Sbjct: 481 RRACPGLSFGLQMVQLILGALLHAFDI 498

BLAST of Bhi03M000519 vs. ExPASy TrEMBL
Match: A0A6J1BSL4 (cytochrome P450 CYP82D47-like OS=Momordica charantia OX=3673 GN=LOC111005334 PE=3 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 2.8e-169
Identity = 312/477 (65.41%), Postives = 363/477 (76.10%), Query Frame = 0

Query: 3   VEAMAELTWNNNQTTVLMGLSILVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPL 62
           +E  A+  W +  TT+L G   ++AI  FR ++K  T + G        KPP V GGWPL
Sbjct: 1   MEPNAQFNWLSLITTLLAG---VIAINFFRFLLKTFTASKG-------LKPPTVAGGWPL 60

Query: 63  IRHLPLFGGPEPPHLKLSALADKYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPR 122
           I HLPL  GPE PH+KLSALA++YGPIFSIQLG   AVVIN+WEAAKQCFTTHD+A+  R
Sbjct: 61  IGHLPLLAGPELPHIKLSALANEYGPIFSIQLGIRKAVVINSWEAAKQCFTTHDVAISSR 120

Query: 123 PKLLSGKLMGYNYAMFIFSPYYNSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKE 182
           P +L+ K++GYNYAMF F P Y+SYWRKMRK T L LLSNRRL+LLR VRESEVN+AIKE
Sbjct: 121 PNILASKILGYNYAMFAFGP-YDSYWRKMRKTTNLHLLSNRRLELLRNVRESEVNQAIKE 180

Query: 183 LY---------QLGGRVEMKQWFGDLIMNLVLLIVGGKRYFGAGAMVEEEEAQRCQKAME 242
           LY         Q    VEMKQW GDL MNL+L +V GKRYFGA AMV E EA+ C +A +
Sbjct: 181 LYTAWKERRDEQGQVLVEMKQWVGDLTMNLILGVVAGKRYFGAAAMVAEAEARACHRATK 240

Query: 243 ESLRLLGMYVLGDAIPYLGWLDVGGHVKAMKRTFKVLDGMLTQWLEEHKKNRA---GKNN 302
           E LRL+G++ LGD +PYLGWLDVGGHVKAMKRT K LD MLT+WLEEH+K+RA    K +
Sbjct: 241 EFLRLMGLFTLGDVVPYLGWLDVGGHVKAMKRTSKELDHMLTRWLEEHRKDRASGRSKED 300

Query: 303 GDFMDVMLSVLEETD----NDILHGFHRDTVIKATCLTMVLGASETSIVAVVWALSLLLN 362
            DFMDVMLSVLEE      +D  H F  DT+IKATCLTMVLG S+T++V V WALSLLLN
Sbjct: 301 KDFMDVMLSVLEEETANVVSDSAHSFDDDTIIKATCLTMVLGGSDTTVVVVTWALSLLLN 360

Query: 363 NRDTLRKVQEELDTHIGRDAKLQESDMEKLVYLEAVVKEAMRLFGAGSLISREAAEDIDV 422
           NR  LRKVQ+ELD  IGRD +L ESD+ KLVY +AVVKE MRL+G   L  RE +ED  +
Sbjct: 361 NRPALRKVQQELDARIGRDRELNESDIGKLVYFQAVVKETMRLYGVVGLFVRETSEDFAI 420

Query: 423 DGFHIKKGTWLFINLWKIHRDERVWDEPLKFKPERFLTTHKNFDVKGGQQFELIPFG 464
           DGFHI+KGTWLF+NLWKIHRDERVW EPL+FKPERFLTTHK+FDVK GQQFELIPFG
Sbjct: 421 DGFHIEKGTWLFMNLWKIHRDERVWGEPLEFKPERFLTTHKDFDVK-GQQFELIPFG 465

BLAST of Bhi03M000519 vs. ExPASy TrEMBL
Match: A0A5E4E6Y6 (PREDICTED: cytochrome P450 OS=Prunus dulcis OX=3755 GN=ALMOND_2B028922 PE=3 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 1.3e-163
Identity = 294/516 (56.98%), Postives = 378/516 (73.26%), Query Frame = 0

Query: 25  LVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPLIRHLPLFGGPEPPHLKLSALAD 84
           ++AI+LF   + + +++    KG   PK P V GGWPL+ HL LF G + PH+ L++L D
Sbjct: 26  ILAILLFSYFIIKRSSSAAKAKG---PKLPKVAGGWPLLGHLGLFRGSQLPHIALASLVD 85

Query: 85  KYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPRPKLLSGKLMGYNYAMFIFSPYY 144
           KYGP F+I +G HSA+VI+ WEAAK CFTT+DI V  RP  L  K +GYN+AMF FSP Y
Sbjct: 86  KYGPAFTINIGIHSALVISTWEAAKDCFTTNDIVVSSRPATLGAKHLGYNFAMFGFSP-Y 145

Query: 145 NSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKELYQL--------GGR-VEMKQW 204
             YWR+MRK+T+L+LLSNRRL+LL++VR SEV  ++KELY L        G R VEMKQW
Sbjct: 146 GPYWREMRKLTSLELLSNRRLELLKKVRVSEVEMSLKELYALWIKRKESSGERLVEMKQW 205

Query: 205 FGDLIMNLVLLIVGGKRYFGAGAMVEEEEAQRCQKAMEESLRLLGMYVLGDAIPYLGWLD 264
           FGDL +N++  +V GKR F  G + EE+EA+R QKAM E   L+G++VLGDA+P+L WLD
Sbjct: 206 FGDLTLNVIFRMVAGKRCFMNGNLSEEKEARRWQKAMREFFHLVGLFVLGDAVPWLSWLD 265

Query: 265 VGGHVKAMKRTFKVLDGMLTQWLEEHKKNRAGKNNGDFMDVMLSVLEETDNDILHGFHRD 324
           +GG  KAMK+T K LDG++ +WLEEHK+ R    + DFMDVMLS ++ TD   + GF  D
Sbjct: 266 LGGQQKAMKKTAKELDGIVAEWLEEHKQKRTKGKDQDFMDVMLSAIDGTD---VAGFDAD 325

Query: 325 TVIKATCLTMVLGASETSIVAVVWALSLLLNNRDTLRKVQEELDTHIGRDAKLQESDMEK 384
           TVIKATCL+++ G S+T++V + W LSLLLNNR  L+KV EELD ++G+   L ESD+  
Sbjct: 326 TVIKATCLSLIAGGSDTTMVTLTWTLSLLLNNRQVLKKVYEELDQYVGKGRLLDESDINN 385

Query: 385 LVYLEAVVKEAMRLFGAGSLI-SREAAEDIDVDGFHIKKGTWLFINLWKIHRDERVWDEP 444
           LVYL+A VKEAMRL  AG L   RE  ED  V G+H+ KGTWL +NLWKI  D RVWD+P
Sbjct: 386 LVYLQATVKEAMRLCPAGPLSGQREFTEDCTVGGYHVPKGTWLLVNLWKIQTDPRVWDDP 445

Query: 445 LKFKPERFLTTHKNFDVKGGQQFELIPFGGGRRICPGLWFGLQLVEFVLGGLLHAFDIWT 504
           ++FKPERFLTTHK+ DV+ GQQFEL+PFG GRR CPG+ FGLQ+    L   LH+FD+ T
Sbjct: 446 MEFKPERFLTTHKDVDVR-GQQFELMPFGSGRRACPGISFGLQMTLLTLASFLHSFDVTT 505

Query: 505 PEDAAVDMTGTIGMTNMKATPLEVFIKPRLSPMLYE 531
            E+  VDMTG+IG+TN+K TPL+V +KPRLSP LY+
Sbjct: 506 QENTPVDMTGSIGLTNIKLTPLDVLVKPRLSPNLYD 533

BLAST of Bhi03M000519 vs. ExPASy TrEMBL
Match: M5XNX5 (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_ppa023495mg PE=3 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 1.5e-162
Identity = 294/516 (56.98%), Postives = 376/516 (72.87%), Query Frame = 0

Query: 25  LVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPLIRHLPLFGGPEPPHLKLSALAD 84
           ++AI+LF   + + +++    KG   PK P V GGWPL+ H  LF G + PH+ L++L D
Sbjct: 15  ILAILLFSYFIIKRSSSAAKAKG---PKLPKVAGGWPLLGHFGLFRGSQLPHIALASLVD 74

Query: 85  KYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPRPKLLSGKLMGYNYAMFIFSPYY 144
           KYGP F+I +G HSA+VI+ WEAAK CFTT+DI V  RP  L  K +GYN+AMF FSP Y
Sbjct: 75  KYGPAFTINIGIHSALVISTWEAAKDCFTTNDIVVSSRPATLGAKHLGYNFAMFGFSP-Y 134

Query: 145 NSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKELYQL--------GGR-VEMKQW 204
            SYWR+MRK+T+L+LLSNRRL+LL++VR SEV  ++KELY L        G R VEMKQW
Sbjct: 135 GSYWREMRKLTSLELLSNRRLELLKKVRVSEVEMSLKELYTLWIKRKESSGERLVEMKQW 194

Query: 205 FGDLIMNLVLLIVGGKRYFGAGAMVEEEEAQRCQKAMEESLRLLGMYVLGDAIPYLGWLD 264
           FGDL +N++  +V GKR F  G + EE+EA+R QKAM E   L G++VLGDA+P+L WLD
Sbjct: 195 FGDLTLNVIFRMVAGKRCFMNGNLSEEKEARRWQKAMREFFHLAGLFVLGDAVPWLSWLD 254

Query: 265 VGGHVKAMKRTFKVLDGMLTQWLEEHKKNRAGKNNGDFMDVMLSVLEETDNDILHGFHRD 324
           +GG  KAMK+T K LD ++ +WLEEHK+ R    + DFMDVMLS ++ TD   + GF  D
Sbjct: 255 LGGQQKAMKKTAKELDSIVAEWLEEHKQKRTKGKDQDFMDVMLSAIDGTD---VAGFDAD 314

Query: 325 TVIKATCLTMVLGASETSIVAVVWALSLLLNNRDTLRKVQEELDTHIGRDAKLQESDMEK 384
           TVIKATCL ++ GAS+T++V + W LSLLLNNR  L+KV EELD ++G+   L ESD+  
Sbjct: 315 TVIKATCLILIAGASDTTMVTLTWTLSLLLNNRQVLKKVYEELDQYVGKGRLLDESDINN 374

Query: 385 LVYLEAVVKEAMRLFGAGSLI-SREAAEDIDVDGFHIKKGTWLFINLWKIHRDERVWDEP 444
           LVYL+A VKEAMRL  AG L   RE  ED  V G+H+ KGTWL +NLWKI  D RVW +P
Sbjct: 375 LVYLQATVKEAMRLCPAGPLSGQREFTEDCTVGGYHVPKGTWLLVNLWKIQTDPRVWADP 434

Query: 445 LKFKPERFLTTHKNFDVKGGQQFELIPFGGGRRICPGLWFGLQLVEFVLGGLLHAFDIWT 504
           ++FKPERFLTTHK+ DV+ GQQFEL+PFG GRR CPG+ FGLQ+    L   LH+FD+ T
Sbjct: 435 MEFKPERFLTTHKDVDVR-GQQFELMPFGSGRRACPGISFGLQMTLLTLASFLHSFDVTT 494

Query: 505 PEDAAVDMTGTIGMTNMKATPLEVFIKPRLSPMLYE 531
            E+A VDMTG+IG+TN+K TPL+V +KPRLSP LY+
Sbjct: 495 QENAPVDMTGSIGLTNIKLTPLDVLVKPRLSPNLYD 522

BLAST of Bhi03M000519 vs. ExPASy TrEMBL
Match: A0A251R9S1 (Uncharacterized protein OS=Prunus persica OX=3760 GN=PRUPE_1G387100 PE=3 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 1.5e-162
Identity = 294/516 (56.98%), Postives = 376/516 (72.87%), Query Frame = 0

Query: 25  LVAIILFRLVMKRVTNNTGGVKGRRNPKPPMVGGGWPLIRHLPLFGGPEPPHLKLSALAD 84
           ++AI+LF   + + +++    KG   PK P V GGWPL+ H  LF G + PH+ L++L D
Sbjct: 26  ILAILLFSYFIIKRSSSAAKAKG---PKLPKVAGGWPLLGHFGLFRGSQLPHIALASLVD 85

Query: 85  KYGPIFSIQLGTHSAVVINNWEAAKQCFTTHDIAVFPRPKLLSGKLMGYNYAMFIFSPYY 144
           KYGP F+I +G HSA+VI+ WEAAK CFTT+DI V  RP  L  K +GYN+AMF FSP Y
Sbjct: 86  KYGPAFTINIGIHSALVISTWEAAKDCFTTNDIVVSSRPATLGAKHLGYNFAMFGFSP-Y 145

Query: 145 NSYWRKMRKITTLDLLSNRRLKLLRQVRESEVNRAIKELYQL--------GGR-VEMKQW 204
            SYWR+MRK+T+L+LLSNRRL+LL++VR SEV  ++KELY L        G R VEMKQW
Sbjct: 146 GSYWREMRKLTSLELLSNRRLELLKKVRVSEVEMSLKELYTLWIKRKESSGERLVEMKQW 205

Query: 205 FGDLIMNLVLLIVGGKRYFGAGAMVEEEEAQRCQKAMEESLRLLGMYVLGDAIPYLGWLD 264
           FGDL +N++  +V GKR F  G + EE+EA+R QKAM E   L G++VLGDA+P+L WLD
Sbjct: 206 FGDLTLNVIFRMVAGKRCFMNGNLSEEKEARRWQKAMREFFHLAGLFVLGDAVPWLSWLD 265

Query: 265 VGGHVKAMKRTFKVLDGMLTQWLEEHKKNRAGKNNGDFMDVMLSVLEETDNDILHGFHRD 324
           +GG  KAMK+T K LD ++ +WLEEHK+ R    + DFMDVMLS ++ TD   + GF  D
Sbjct: 266 LGGQQKAMKKTAKELDSIVAEWLEEHKQKRTKGKDQDFMDVMLSAIDGTD---VAGFDAD 325

Query: 325 TVIKATCLTMVLGASETSIVAVVWALSLLLNNRDTLRKVQEELDTHIGRDAKLQESDMEK 384
           TVIKATCL ++ GAS+T++V + W LSLLLNNR  L+KV EELD ++G+   L ESD+  
Sbjct: 326 TVIKATCLILIAGASDTTMVTLTWTLSLLLNNRQVLKKVYEELDQYVGKGRLLDESDINN 385

Query: 385 LVYLEAVVKEAMRLFGAGSLI-SREAAEDIDVDGFHIKKGTWLFINLWKIHRDERVWDEP 444
           LVYL+A VKEAMRL  AG L   RE  ED  V G+H+ KGTWL +NLWKI  D RVW +P
Sbjct: 386 LVYLQATVKEAMRLCPAGPLSGQREFTEDCTVGGYHVPKGTWLLVNLWKIQTDPRVWADP 445

Query: 445 LKFKPERFLTTHKNFDVKGGQQFELIPFGGGRRICPGLWFGLQLVEFVLGGLLHAFDIWT 504
           ++FKPERFLTTHK+ DV+ GQQFEL+PFG GRR CPG+ FGLQ+    L   LH+FD+ T
Sbjct: 446 MEFKPERFLTTHKDVDVR-GQQFELMPFGSGRRACPGISFGLQMTLLTLASFLHSFDVTT 505

Query: 505 PEDAAVDMTGTIGMTNMKATPLEVFIKPRLSPMLYE 531
            E+A VDMTG+IG+TN+K TPL+V +KPRLSP LY+
Sbjct: 506 QENAPVDMTGSIGLTNIKLTPLDVLVKPRLSPNLYD 533