Bhi03M000400 (mRNA) Wax gourd (B227) v1

Overview
NameBhi03M000400
TypemRNA
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionCysteine rich receptor like kinase
Locationchr3: 10207793 .. 10210552 (-)
Sequence length1425
RNA-Seq ExpressionBhi03M000400
SyntenyBhi03M000400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTACGCTATTCAAGTCGTCCCATATTTGGTGCAATGGAAGATGAACCCGTATTCATCATGTGGATAAAAAACGACACTATAGCACAGGAAGATCTGTTCAGGCAGAATCTGGGGGCATTGCTGGATGGCTTGAAAGATAATGCATCTTCTGGTGGTTCTTCATTGAAGTATGCAGCTGGAGATATATTTGGAGAAGCGTTTGGCAGGATATATGCACTTCTGCAGTGCACACCTGATTTGTCTAAATTACAATGTGATGCATACTTAGATTCAGCGTTTCAAACAATTCCATCGTGTTGTAGTAAAACTTGGAGATATTATTCACCCAGCTGTAATGTTAGATATCAGATGTCCCTCTTCTTTGACCCTTCTGCTGATTTGCCACCACCACCGCCGCTAATTTTTGTTCCTTCTAGCAATTCCAGGTCTTCAGAAGGTATGGTTTGAGGTTGTGTTCACTTTGTTCTTATCAATTCAACCATTTTCTTTTCTCGTGTCTTTAAGGATCATTTTAAGCATCAAAAGTGATCTATACAGTCCGTATTTCAACACATTCTCTAAATGAAAATTTTAGGTTTCGTTGGATAGCTATTTAGTTTTCTAGCTTTGAAACATTGTGCTAATTCCCTCCCAATTTCTCTGCAATAATTTCCAACTTTTTTATGAAAATATTTGAATTCTTTAACCAAAATGAGTATAAGTCAACTGATATAAAACTTGTACTATTAATCTCGATGTTAGATGTTTGATCCCTTCACTTCACATATTTTCATATATATATTATTAGTTCTTAGCAAAATTTTAAAAAATAATAAATAAATAAATAAGTAAATTACTTTCAATCGTTGACATGGATTTTGAAGACACTTGTTAAAAATAGATACCAAAAAAAAAAACTGTGATTGGAAGTAATATTTACAAACTTATTTTTCATAAACCAAAAAACAAATGATTATCAAAAGATACCTGGCATTATTGTTTAAAATAATTAGTTCAAGAATTTAACAGAAAGAGATGTCAATTGTTTTCAGCAAAGAAGAACACCATAAACACTACCATTGTAATAGTTCTCCCATCTGTGGCTGTTTTGGTTTTAGCCGTCGGCATCTTCACCTTTTTGAGATCCAGAAAGTTGAGGGAAAGATTTGGGAGTAAGATCATATTTTTTTTCTTCAAAGCCTAATATTTCTTCTTATAATTATCATTTTGAAATGGTAAATGTCTGCTAAATTTCTAATTCTCATGTCAACATACTACTTGTGAATTGTGAAGCTAATGATGATGACGTTATCCCCACTTTCTTGCAATATGGATTCAAGACGATTAGAGAAGCAACAAATGACTTCTCAATTTCAAATAAACTTGGACAAGGTGGATTTGGTGGTGTTTATAAGGTAATTGCATGGAATTGAACAATCTTAAAATGTCAAAGTATAGCATTTTGCTCCAACAGGGTGGAAGACTCCTATAATTTTACCCTTGTTTATGTACCTTCAGGGTAAGCTTTTCAATGGACAAGAAGTGGCTGTGAAAAGGCTAGCTCAAGGTTCGCAACAGGGTGACTTTGAATTTAAAAATGAAGTTTTACTAGTTGCTAAGCTGCAACACCGGAATCTGGTTCGACTTCTTGGCTTTTGCTTCGAGAGAAACGAAAGGCTACTCATCTACGAGTTTTTGCTTAACTCAAGCCTGGATCATTTCATATTTGGTAACCTCTTTGTATTTTGTTCTCTGTTCTTAGGCATCATTTCATAATCTGTCTATCCAGTTAAAAGTTTGTTCACATTTTCAGTTCACTTTTAGCTAAAAGCATACATATACTTATTAAATGAAAATGTAGATCCCATTCAACGTCATTATCTGGACTGGTCAAGACGCTACAAGATTATAGTTGGCATCAGTCGTGGACTCGTATATTTGCACGGTGATTCTCGATTCAAAGTCATTCATCATGATCTTAAAACTAGTAATATTTTATTAGATGCAGAGCTAAATCCAAAGATCACTGATTTTGGAATGATAAAATTGTGTTCAATCAATCGATCTCATGGTGATACGAGTAAAATTAAGGGAACATAGTAAGTTCTAAAAACTGTTGCACTCTATTATCATTCAAATTCTGTATTTTCTACAAAATTTAGGTTGTCTTACAAGATTGAGACAAATACTGAACTAAATGTTTCTTATTTCAAGTGGATATATGGCCCAGAGTACGCAATGTATGGATATTTTTCTGTGAAAACGTATGTGTTCAGTTTTGATGTAATTCTCCTAGAGATTGTAAGTGGCCAAAAGAACAGTTGCTTCCAAGATGGGGAAAATATAGAGCACCTTCTAAGCTATGTAAGTATGAATATTTTTGAAAGATATGTTCTTTCACTACAAAGTTTTAACACAAATTTGCCAAAACAGACATGGAAAAACTGGATGGAAGGGAATGTCATAGCCATCATAGACCCGATATTGGCTACAACTTGCATTGATGAAATCGTGAGATGCATCCATCTTGGATTACTGTGTGTTCAAGAAAAGGTAGCTAGTAGACCAACCATGGATTCAGTTGTTCTCATGCTCAATAGCAACTCCCTCGCTCTACCTGAACATTTGCGGCCCGGATTTTTGCTGCAGAGCAATACCTCAAACTTGCCACGGCATCTTGATGACACAGAAAGATCACAGCTTAGTCTATGCGAGAGTCTTAACATAGAAGAAGAATCAGCAAACCAGTATTCAGCTATTGATATTCAAGCTTACTAG

mRNA sequence

ATGCTACGCTATTCAAGTCGTCCCATATTTGGTGCAATGGAAGATGAACCCGTATTCATCATGTGGATAAAAAACGACACTATAGCACAGGAAGATCTGTTCAGGCAGAATCTGGGGGCATTGCTGGATGGCTTGAAAGATAATGCATCTTCTGGTGGTTCTTCATTGAAGTATGCAGCTGGAGATATATTTGGAGAAGCGTTTGGCAGGATATATGCACTTCTGCAGTGCACACCTGATTTGTCTAAATTACAATGTGATGCATACTTAGATTCAGCGTTTCAAACAATTCCATCGTGTTGTAGTAAAACTTGGAGATATTATTCACCCAGCTGTAATGTTAGATATCAGATGTCCCTCTTCTTTGACCCTTCTGCTGATTTGCCACCACCACCGCCGCTAATTTTTGTTCCTTCTAGCAATTCCAGGTCTTCAGAAGCCCCTAATATTTCTTCTTATAATTATCATTTTGAAATGGGTAAGCTTTTCAATGGACAAGAAGTGGCTGTGAAAAGGCTAGCTCAAGGTTCGCAACAGGGTGACTTTGAATTTAAAAATGAAGTTTTACTAGTTGCTAAGCTGCAACACCGGAATCTGGTTCGACTTCTTGGCTTTTGCTTCGAGAGAAACGAAAGGCTACTCATCTACGAGTTTTTGCTTAACTCAAGCCTGGATCATTTCATATTTGATCCCATTCAACGTCATTATCTGGACTGGTCAAGACGCTACAAGATTATAGTTGGCATCAGTCGTGGACTCGTATATTTGCACGGTGATTCTCGATTCAAAGTCATTCATCATGATCTTAAAACTAGTAATATTTTATTAGATGCAGAGCTAAATCCAAAGATCACTGATTTTGGAATGATAAAATTGTGTTCAATCAATCGATCTCATGGTGATACGAGTAAAATTAAGGGAACATATGGATATATGGCCCAGAAGATTGTAAGTGGCCAAAAGAACAGTTGCTTCCAAGATGGGGAAAATATAGAGCACCTTCTAAGCTATGTAAGTATGAATATTTTTGAAAGATATGTTCTTTCACTACAAAGTTTTAACACAAATTTGCCAAAACAGACATGGAAAAACTGGATGGAAGGGAATGTCATAGCCATCATAGACCCGATATTGGCTACAACTTGCATTGATGAAATCGTGAGATGCATCCATCTTGGATTACTGTGTGTTCAAGAAAAGGTAGCTAGTAGACCAACCATGGATTCAGTTGTTCTCATGCTCAATAGCAACTCCCTCGCTCTACCTGAACATTTGCGGCCCGGATTTTTGCTGCAGAGCAATACCTCAAACTTGCCACGGCATCTTGATGACACAGAAAGATCACAGCTTAGTCTATGCGAGAGTCTTAACATAGAAGAAGAATCAGCAAACCAGTATTCAGCTATTGATATTCAAGCTTACTAG

Coding sequence (CDS)

ATGCTACGCTATTCAAGTCGTCCCATATTTGGTGCAATGGAAGATGAACCCGTATTCATCATGTGGATAAAAAACGACACTATAGCACAGGAAGATCTGTTCAGGCAGAATCTGGGGGCATTGCTGGATGGCTTGAAAGATAATGCATCTTCTGGTGGTTCTTCATTGAAGTATGCAGCTGGAGATATATTTGGAGAAGCGTTTGGCAGGATATATGCACTTCTGCAGTGCACACCTGATTTGTCTAAATTACAATGTGATGCATACTTAGATTCAGCGTTTCAAACAATTCCATCGTGTTGTAGTAAAACTTGGAGATATTATTCACCCAGCTGTAATGTTAGATATCAGATGTCCCTCTTCTTTGACCCTTCTGCTGATTTGCCACCACCACCGCCGCTAATTTTTGTTCCTTCTAGCAATTCCAGGTCTTCAGAAGCCCCTAATATTTCTTCTTATAATTATCATTTTGAAATGGGTAAGCTTTTCAATGGACAAGAAGTGGCTGTGAAAAGGCTAGCTCAAGGTTCGCAACAGGGTGACTTTGAATTTAAAAATGAAGTTTTACTAGTTGCTAAGCTGCAACACCGGAATCTGGTTCGACTTCTTGGCTTTTGCTTCGAGAGAAACGAAAGGCTACTCATCTACGAGTTTTTGCTTAACTCAAGCCTGGATCATTTCATATTTGATCCCATTCAACGTCATTATCTGGACTGGTCAAGACGCTACAAGATTATAGTTGGCATCAGTCGTGGACTCGTATATTTGCACGGTGATTCTCGATTCAAAGTCATTCATCATGATCTTAAAACTAGTAATATTTTATTAGATGCAGAGCTAAATCCAAAGATCACTGATTTTGGAATGATAAAATTGTGTTCAATCAATCGATCTCATGGTGATACGAGTAAAATTAAGGGAACATATGGATATATGGCCCAGAAGATTGTAAGTGGCCAAAAGAACAGTTGCTTCCAAGATGGGGAAAATATAGAGCACCTTCTAAGCTATGTAAGTATGAATATTTTTGAAAGATATGTTCTTTCACTACAAAGTTTTAACACAAATTTGCCAAAACAGACATGGAAAAACTGGATGGAAGGGAATGTCATAGCCATCATAGACCCGATATTGGCTACAACTTGCATTGATGAAATCGTGAGATGCATCCATCTTGGATTACTGTGTGTTCAAGAAAAGGTAGCTAGTAGACCAACCATGGATTCAGTTGTTCTCATGCTCAATAGCAACTCCCTCGCTCTACCTGAACATTTGCGGCCCGGATTTTTGCTGCAGAGCAATACCTCAAACTTGCCACGGCATCTTGATGACACAGAAAGATCACAGCTTAGTCTATGCGAGAGTCTTAACATAGAAGAAGAATCAGCAAACCAGTATTCAGCTATTGATATTCAAGCTTACTAG

Protein sequence

MLRYSSRPIFGAMEDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLKYAAGDIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKTWRYYSPSCNVRYQMSLFFDPSADLPPPPPLIFVPSSNSRSSEAPNISSYNYHFEMGKLFNGQEVAVKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLLNSSLDHFIFDPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAELNPKITDFGMIKLCSINRSHGDTSKIKGTYGYMAQKIVSGQKNSCFQDGENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPILATTCIDEIVRCIHLGLLCVQEKVASRPTMDSVVLMLNSNSLALPEHLRPGFLLQSNTSNLPRHLDDTERSQLSLCESLNIEEESANQYSAIDIQAY
Homology
BLAST of Bhi03M000400 vs. TAIR 10
Match: AT4G21410.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 29 )

HSP 1 Score: 336.7 bits (862), Expect = 3.1e-92
Identity = 213/528 (40.34%), Postives = 275/528 (52.08%), Query Frame = 0

Query: 1   MLRYSSRPIFGAMEDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLKYAA 60
           M RYS+R I+G  E  P        +  A  D F +    LLD LK  A++GG + KYA 
Sbjct: 126 MFRYSNRTIYGRKETNPTKAFIAGEEISANRDDFERLQRGLLDRLKGIAAAGGPNRKYAQ 185

Query: 61  GDIFGEA-FGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKT--WRYYSPSCNVRYQ 120
           G+    A + R Y  +QCTPDLS+  C+  L   F+ IPSCC      R++SPSCN R++
Sbjct: 186 GNGSASAGYRRFYGTVQCTPDLSEQDCNDCLVFGFENIPSCCDAEIGLRWFSPSCNFRFE 245

Query: 121 MSLFFDPSADLPPPPP----------------------------LIFVP----------- 180
              F++  ADL P PP                             I +P           
Sbjct: 246 TWRFYEFDADLEPDPPAIQPADSPQSAARTERTGKGKGGSKVIIAIVIPILLVALLAICL 305

Query: 181 -----------------------SSNSRSSEAPNISSYNYHFE----------------- 240
                                  S +    E  N  S   HFE                 
Sbjct: 306 CLVLKWRKNKSGYKNKVLGKSPLSGSIAEDEFSNTESLLVHFETLKTATDNFSSENELGR 365

Query: 241 -------MGKLFNGQEVAVKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNE 300
                   G    GQE+AVKRL+  S QGD EFKNE+LL+AKLQHRNLVRL+GFC +  E
Sbjct: 366 GGFGSVYKGVFPQGQEIAVKRLSGNSGQGDNEFKNEILLLAKLQHRNLVRLIGFCIQGEE 425

Query: 301 RLLIYEFLLNSSLDHFIFDPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKT 360
           RLL+YEF+ N+SLD FIFD  +R  LDW  RYK+I GI+RGL+YLH DSRF++IH DLK 
Sbjct: 426 RLLVYEFIKNASLDQFIFDTEKRQLLDWVVRYKMIGGIARGLLYLHEDSRFRIIHRDLKA 485

Query: 361 SNILLDAELNPKITDFGMIKLCSINR--SHGDTSKIKGTYGYMA-QKIVSGQ---KNSCF 420
           SNILLD E+NPKI DFG+ KL    +  +H  TS+I GTYGYMA +  + GQ   K   F
Sbjct: 486 SNILLDQEMNPKIADFGLAKLFDSGQTMTHRFTSRIAGTYGYMAPEYAMHGQFSVKTDVF 545

Query: 421 QDGENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPILATTCIDE 434
             G  +  +++    N       +      +L    W++W E  ++++IDP L     +E
Sbjct: 546 SFGVLVIEIITGKRNN---NGGSNGDEDAEDLLSWVWRSWREDTILSVIDPSLTAGSRNE 605

BLAST of Bhi03M000400 vs. TAIR 10
Match: AT4G38830.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 26 )

HSP 1 Score: 312.0 bits (798), Expect = 8.2e-85
Identity = 206/539 (38.22%), Postives = 279/539 (51.76%), Query Frame = 0

Query: 3   RYSSRPIFGAMEDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLK-YAAG 62
           RYS+R IF  +E  P   +    +     D + ++L  LL+GLK+ AS  G S K +  G
Sbjct: 123 RYSNRTIFNRLEISPHTSITGTRNFTGDRDSWEKSLRGLLEGLKNRASVIGRSKKNFVVG 182

Query: 63  DIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKTWRYY--SPSCNVRYQMS 122
           +  G +F  ++ L+QCTPD+S+  C   L      IPSCC      Y  SPSC + Y   
Sbjct: 183 ETSGPSFQTLFGLVQCTPDISEEDCSYCLSQGIAKIPSCCDMKMGSYVMSPSCMLAYAPW 242

Query: 123 LFFDP-----------SADLPP----------------PPPLIFVPSS------------ 182
            F+DP           +   PP                P  LIF  +S            
Sbjct: 243 RFYDPVDTDDPSSVPATPSRPPKNETRSVTQGDKNRGVPKALIFASASVAIVVLFIVLLV 302

Query: 183 ---------NSRSS----EAPNISSYNYHFEM--------------------------GK 242
                    N R+S    E  NIS+ +  F+                           G 
Sbjct: 303 VFLKLRRKENIRNSENKHENENISTDSMKFDFSVLQDATSHFSLENKLGEGGFGAVYKGV 362

Query: 243 LFNGQEVAVKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLLN 302
           L +GQ++AVKRL++ +QQG+ EFKNE LLVAKLQHRNLV+LLG+  E  ERLL+YEFL +
Sbjct: 363 LSDGQKIAVKRLSKNAQQGETEFKNEFLLVAKLQHRNLVKLLGYSIEGTERLLVYEFLPH 422

Query: 303 SSLDHFIFDPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAELN 362
           +SLD FIFDPIQ + L+W  RYKII G++RGL+YLH DSR ++IH DLK SNILLD E+ 
Sbjct: 423 TSLDKFIFDPIQGNELEWEIRYKIIGGVARGLLYLHQDSRLRIIHRDLKASNILLDEEMT 482

Query: 363 PKITDFGMIKLCSINR-SHGDTSKIKGTYGYMAQ----------------------KIVS 422
           PKI DFGM +L  I+  +   T++I GT+GYMA                       +I+S
Sbjct: 483 PKIADFGMARLFDIDHTTQRYTNRIVGTFGYMAPEYVMHGQFSFKTDVYSFGVLVLEIIS 542

Query: 423 GQKNSCFQDGENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPIL 435
           G+KNS F   +++  L+S+                        W+NW EG  + ++D IL
Sbjct: 543 GKKNSGFSSEDSMGDLISF-----------------------AWRNWKEGVALNLVDKIL 602

BLAST of Bhi03M000400 vs. TAIR 10
Match: AT4G00970.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 41 )

HSP 1 Score: 303.5 bits (776), Expect = 2.9e-82
Identity = 203/546 (37.18%), Postives = 278/546 (50.92%), Query Frame = 0

Query: 1   MLRYSSRPIFGAMEDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLKYAA 60
           M RYS +PIFG +E  PV      ++     + F +    LL+ L+  A+SGGS  KYA 
Sbjct: 140 MFRYSDKPIFGKLETSPVLEAPNPSNATGDRNEFIRLQSELLNRLRSMAASGGSKRKYAQ 199

Query: 61  G-DIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKTWRYYSPSCNVRYQMS 120
           G D     +   +  +QCTPDLS+  C+  L   F           R++ PSCN + +  
Sbjct: 200 GTDPGSPPYTTFFGAVQCTPDLSEKDCNDCLSYGFSNATK-GRVGIRWFCPSCNFQIESD 259

Query: 121 L-FFDPSADLPPPPP-------------------------LIFVPSSNSRSSEAPN---- 180
           L FF   ++  P P                          L F  + N R+++  +    
Sbjct: 260 LRFFLLDSEYEPDPKPGNDKVKIIIATVCSVIGFAIIAVFLYFFMTRNRRTAKQRHEGKD 319

Query: 181 ----------------------ISSYNYHFEMGK----------LFNGQEVAVKRLAQGS 240
                                  + ++   ++G+          L  G+E+AVKRL+  S
Sbjct: 320 LEELMIKDAQLLQLDFDTIRLATNDFSRDNQLGEGGFGAVYKGVLDYGEEIAVKRLSMKS 379

Query: 241 QQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLLNSSLDHFIFDPIQRHYL 300
            QGD EF NEV LVAKLQHRNLVRLLGFC +  ER+LIYEF  N+SLDH+IFD  +R  L
Sbjct: 380 GQGDNEFINEVSLVAKLQHRNLVRLLGFCLQGEERILIYEFFKNTSLDHYIFDSNRRMIL 439

Query: 301 DWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAELNPKITDFGMIKLCSINR 360
           DW  RY+II G++RGL+YLH DSRFK++H D+K SN+LLD  +NPKI DFGM KL   ++
Sbjct: 440 DWETRYRIISGVARGLLYLHEDSRFKIVHRDMKASNVLLDDAMNPKIADFGMAKLFDTDQ 499

Query: 361 SHGD--TSKIKGTYGYMAQ----------------------KIVSGQKNSCFQDGENIEH 420
           +     TSK+ GTYGYMA                       +I+ G+KN+   + ++   
Sbjct: 500 TSQTRFTSKVAGTYGYMAPEYAMSGEFSVKTDVFSFGVLVLEIIKGKKNNWSPEEDSSLF 559

Query: 421 LLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPILATT--CIDEIVRCIH 458
           LLSYV                       WK+W EG V+ I+DP L  T    DEI++CIH
Sbjct: 560 LLSYV-----------------------WKSWREGEVLNIVDPSLVETIGVSDEIMKCIH 619

BLAST of Bhi03M000400 vs. TAIR 10
Match: AT4G05200.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 25 )

HSP 1 Score: 298.9 bits (764), Expect = 7.2e-81
Identity = 186/537 (34.64%), Postives = 282/537 (52.51%), Query Frame = 0

Query: 1   MLRYSSRPIFGAMEDEP-VFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLKYA 60
           M+RYS++ I G M   P VF+   +N T  Q   F ++L ALL  +   A+   SS K+A
Sbjct: 126 MVRYSNQSIVGQMRIRPGVFLTNKQNITENQVSRFNESLPALLIDVAVKAAL--SSRKFA 185

Query: 61  AGDIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKT--WRYYSPSCNVRYQ 120
                   F  IY+L+QCTPDL+   C++ L      +P CC ++   R  +PSC+ RY+
Sbjct: 186 TEKANFTVFQTIYSLVQCTPDLTNQDCESCLRQVINYLPRCCDRSVGGRVIAPSCSFRYE 245

Query: 121 MSLFFD---PSADLPPPP------PLIFVPS----------------------------- 180
           +  F++    +A + PPP      P + +PS                             
Sbjct: 246 LYPFYNETIAAAPMAPPPSSTVTAPPLNIPSEKGKGKNLTVIVTAIAVPVSVCVLLLGAM 305

Query: 181 --------SNSRSSEAPNI------SSYNYHFEM-------------------------- 240
                   +N  S+E  ++      S+    F+                           
Sbjct: 306 CWLLARRRNNKLSAETEDLDEDGITSTETLQFQFSAIEAATNKFSESNKLGHGGFGEVYK 365

Query: 241 GKLFNGQEVAVKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFL 300
           G+L  G+ VA+KRL+QGS QG  EFKNEV +VAKLQHRNL +LLG+C +  E++L+YEF+
Sbjct: 366 GQLITGETVAIKRLSQGSTQGAEEFKNEVDVVAKLQHRNLAKLLGYCLDGEEKILVYEFV 425

Query: 301 LNSSLDHFIFDPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAE 360
            N SLD+F+FD  +R  LDW RRYKII GI+RG++YLH DSR  +IH DLK SNILLDA+
Sbjct: 426 PNKSLDYFLFDNEKRRVLDWQRRYKIIEGIARGILYLHRDSRLTIIHRDLKASNILLDAD 485

Query: 361 LNPKITDFGMIKLCSINRSHGDTSKIKGTYGYMAQ----------------------KIV 420
           ++PKI+DFGM ++  ++++  +T +I GTYGYM+                       +++
Sbjct: 486 MHPKISDFGMARIFGVDQTQANTKRIVGTYGYMSPEYAIHGKYSVKSDVYSFGVLVLELI 545

Query: 421 SGQKNSCFQDGENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPI 434
           +G+KNS F + + +  L++YV                       WK W+E + + ++D  
Sbjct: 546 TGKKNSSFYEEDGLGDLVTYV-----------------------WKLWVENSPLELVDEA 605

BLAST of Bhi03M000400 vs. TAIR 10
Match: AT4G23180.1 (cysteine-rich RLK (RECEPTOR-like protein kinase) 10 )

HSP 1 Score: 298.5 bits (763), Expect = 9.4e-81
Identity = 198/559 (35.42%), Postives = 274/559 (49.02%), Query Frame = 0

Query: 1   MLRYSSRPIFGAM-EDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSG-GSSLKY 60
           +LRYS++ I   +     V ++  +N T  Q DL       +L  L   A+    SS K+
Sbjct: 134 VLRYSNQNILSTLITTGGVILVNTRNVTSNQLDLLSD---LVLPTLNQAATVALNSSKKF 193

Query: 61  AAGDIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKTWRYYSPSCNVRYQM 120
                   A    Y L+QCTPDL++  C   L      IP+      R  +PSC  RY++
Sbjct: 194 GTRKNNFTALQSFYGLVQCTPDLTRQDCSRCLQLVINQIPTDRIGA-RIINPSCTSRYEI 253

Query: 121 SLFFDPSA-DLPPPPPLIFVP--SSNSRSSEAPN-------------------ISSYNY- 180
             F+  SA   PPPPP I  P  S+  RS +  N                   I+ Y + 
Sbjct: 254 YAFYTESAVPPPPPPPSISTPPVSAPPRSGKDGNSKVLVIAIVVPIIVAVLLFIAGYCFL 313

Query: 181 ---------------------------------------------------HFEMGKLFN 240
                                                                  G L +
Sbjct: 314 TRRARKSYYTPSAFAGDDITTADSLQLDYRTIQTATDDFVESNKIGQGGFGEVYKGTLSD 373

Query: 241 GQEVAVKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLLNSSL 300
           G EVAVKRL++ S QG+ EFKNEV+LVAKLQHRNLVRLLGFC +  ER+L+YE++ N SL
Sbjct: 374 GTEVAVKRLSKSSGQGEVEFKNEVVLVAKLQHRNLVRLLGFCLDGEERVLVYEYVPNKSL 433

Query: 301 DHFIFDPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAELNPKI 360
           D+F+FDP ++  LDW+RRYKII G++RG++YLH DSR  +IH DLK SNILLDA++NPKI
Sbjct: 434 DYFLFDPAKKGQLDWTRRYKIIGGVARGILYLHQDSRLTIIHRDLKASNILLDADMNPKI 493

Query: 361 TDFGMIKLCSINRSHGDTSKIKGTYGYMAQ----------------------KIVSGQKN 420
            DFGM ++  ++++  +TS+I GTYGYM+                       +I+SG+KN
Sbjct: 494 ADFGMARIFGLDQTEENTSRIVGTYGYMSPEYAMHGQYSMKSDVYSFGVLVLEIISGKKN 553

Query: 421 SCFQDGENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPILATTC 461
           S F   +    L+SY                        W  W  G  + ++DP +   C
Sbjct: 554 SSFYQTDGAHDLVSY-----------------------AWGLWSNGRPLELVDPAIVENC 613

BLAST of Bhi03M000400 vs. ExPASy Swiss-Prot
Match: Q8S9L6 (Cysteine-rich receptor-like protein kinase 29 OS=Arabidopsis thaliana OX=3702 GN=CRK29 PE=2 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 4.4e-91
Identity = 213/528 (40.34%), Postives = 275/528 (52.08%), Query Frame = 0

Query: 1   MLRYSSRPIFGAMEDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLKYAA 60
           M RYS+R I+G  E  P        +  A  D F +    LLD LK  A++GG + KYA 
Sbjct: 126 MFRYSNRTIYGRKETNPTKAFIAGEEISANRDDFERLQRGLLDRLKGIAAAGGPNRKYAQ 185

Query: 61  GDIFGEA-FGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKT--WRYYSPSCNVRYQ 120
           G+    A + R Y  +QCTPDLS+  C+  L   F+ IPSCC      R++SPSCN R++
Sbjct: 186 GNGSASAGYRRFYGTVQCTPDLSEQDCNDCLVFGFENIPSCCDAEIGLRWFSPSCNFRFE 245

Query: 121 MSLFFDPSADLPPPPP----------------------------LIFVP----------- 180
              F++  ADL P PP                             I +P           
Sbjct: 246 TWRFYEFDADLEPDPPAIQPADSPQSAARTERTGKGKGGSKVIIAIVIPILLVALLAICL 305

Query: 181 -----------------------SSNSRSSEAPNISSYNYHFE----------------- 240
                                  S +    E  N  S   HFE                 
Sbjct: 306 CLVLKWRKNKSGYKNKVLGKSPLSGSIAEDEFSNTESLLVHFETLKTATDNFSSENELGR 365

Query: 241 -------MGKLFNGQEVAVKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNE 300
                   G    GQE+AVKRL+  S QGD EFKNE+LL+AKLQHRNLVRL+GFC +  E
Sbjct: 366 GGFGSVYKGVFPQGQEIAVKRLSGNSGQGDNEFKNEILLLAKLQHRNLVRLIGFCIQGEE 425

Query: 301 RLLIYEFLLNSSLDHFIFDPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKT 360
           RLL+YEF+ N+SLD FIFD  +R  LDW  RYK+I GI+RGL+YLH DSRF++IH DLK 
Sbjct: 426 RLLVYEFIKNASLDQFIFDTEKRQLLDWVVRYKMIGGIARGLLYLHEDSRFRIIHRDLKA 485

Query: 361 SNILLDAELNPKITDFGMIKLCSINR--SHGDTSKIKGTYGYMA-QKIVSGQ---KNSCF 420
           SNILLD E+NPKI DFG+ KL    +  +H  TS+I GTYGYMA +  + GQ   K   F
Sbjct: 486 SNILLDQEMNPKIADFGLAKLFDSGQTMTHRFTSRIAGTYGYMAPEYAMHGQFSVKTDVF 545

Query: 421 QDGENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPILATTCIDE 434
             G  +  +++    N       +      +L    W++W E  ++++IDP L     +E
Sbjct: 546 SFGVLVIEIITGKRNN---NGGSNGDEDAEDLLSWVWRSWREDTILSVIDPSLTAGSRNE 605

BLAST of Bhi03M000400 vs. ExPASy Swiss-Prot
Match: Q9T0J1 (Cysteine-rich receptor-like protein kinase 26 OS=Arabidopsis thaliana OX=3702 GN=CRK26 PE=2 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.2e-83
Identity = 206/539 (38.22%), Postives = 279/539 (51.76%), Query Frame = 0

Query: 3   RYSSRPIFGAMEDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLK-YAAG 62
           RYS+R IF  +E  P   +    +     D + ++L  LL+GLK+ AS  G S K +  G
Sbjct: 123 RYSNRTIFNRLEISPHTSITGTRNFTGDRDSWEKSLRGLLEGLKNRASVIGRSKKNFVVG 182

Query: 63  DIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKTWRYY--SPSCNVRYQMS 122
           +  G +F  ++ L+QCTPD+S+  C   L      IPSCC      Y  SPSC + Y   
Sbjct: 183 ETSGPSFQTLFGLVQCTPDISEEDCSYCLSQGIAKIPSCCDMKMGSYVMSPSCMLAYAPW 242

Query: 123 LFFDP-----------SADLPP----------------PPPLIFVPSS------------ 182
            F+DP           +   PP                P  LIF  +S            
Sbjct: 243 RFYDPVDTDDPSSVPATPSRPPKNETRSVTQGDKNRGVPKALIFASASVAIVVLFIVLLV 302

Query: 183 ---------NSRSS----EAPNISSYNYHFEM--------------------------GK 242
                    N R+S    E  NIS+ +  F+                           G 
Sbjct: 303 VFLKLRRKENIRNSENKHENENISTDSMKFDFSVLQDATSHFSLENKLGEGGFGAVYKGV 362

Query: 243 LFNGQEVAVKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLLN 302
           L +GQ++AVKRL++ +QQG+ EFKNE LLVAKLQHRNLV+LLG+  E  ERLL+YEFL +
Sbjct: 363 LSDGQKIAVKRLSKNAQQGETEFKNEFLLVAKLQHRNLVKLLGYSIEGTERLLVYEFLPH 422

Query: 303 SSLDHFIFDPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAELN 362
           +SLD FIFDPIQ + L+W  RYKII G++RGL+YLH DSR ++IH DLK SNILLD E+ 
Sbjct: 423 TSLDKFIFDPIQGNELEWEIRYKIIGGVARGLLYLHQDSRLRIIHRDLKASNILLDEEMT 482

Query: 363 PKITDFGMIKLCSINR-SHGDTSKIKGTYGYMAQ----------------------KIVS 422
           PKI DFGM +L  I+  +   T++I GT+GYMA                       +I+S
Sbjct: 483 PKIADFGMARLFDIDHTTQRYTNRIVGTFGYMAPEYVMHGQFSFKTDVYSFGVLVLEIIS 542

Query: 423 GQKNSCFQDGENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPIL 435
           G+KNS F   +++  L+S+                        W+NW EG  + ++D IL
Sbjct: 543 GKKNSGFSSEDSMGDLISF-----------------------AWRNWKEGVALNLVDKIL 602

BLAST of Bhi03M000400 vs. ExPASy Swiss-Prot
Match: O23081 (Cysteine-rich receptor-like protein kinase 41 OS=Arabidopsis thaliana OX=3702 GN=CRK41 PE=3 SV=2)

HSP 1 Score: 303.5 bits (776), Expect = 4.1e-81
Identity = 203/546 (37.18%), Postives = 278/546 (50.92%), Query Frame = 0

Query: 1   MLRYSSRPIFGAMEDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLKYAA 60
           M RYS +PIFG +E  PV      ++     + F +    LL+ L+  A+SGGS  KYA 
Sbjct: 140 MFRYSDKPIFGKLETSPVLEAPNPSNATGDRNEFIRLQSELLNRLRSMAASGGSKRKYAQ 199

Query: 61  G-DIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKTWRYYSPSCNVRYQMS 120
           G D     +   +  +QCTPDLS+  C+  L   F           R++ PSCN + +  
Sbjct: 200 GTDPGSPPYTTFFGAVQCTPDLSEKDCNDCLSYGFSNATK-GRVGIRWFCPSCNFQIESD 259

Query: 121 L-FFDPSADLPPPPP-------------------------LIFVPSSNSRSSEAPN---- 180
           L FF   ++  P P                          L F  + N R+++  +    
Sbjct: 260 LRFFLLDSEYEPDPKPGNDKVKIIIATVCSVIGFAIIAVFLYFFMTRNRRTAKQRHEGKD 319

Query: 181 ----------------------ISSYNYHFEMGK----------LFNGQEVAVKRLAQGS 240
                                  + ++   ++G+          L  G+E+AVKRL+  S
Sbjct: 320 LEELMIKDAQLLQLDFDTIRLATNDFSRDNQLGEGGFGAVYKGVLDYGEEIAVKRLSMKS 379

Query: 241 QQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLLNSSLDHFIFDPIQRHYL 300
            QGD EF NEV LVAKLQHRNLVRLLGFC +  ER+LIYEF  N+SLDH+IFD  +R  L
Sbjct: 380 GQGDNEFINEVSLVAKLQHRNLVRLLGFCLQGEERILIYEFFKNTSLDHYIFDSNRRMIL 439

Query: 301 DWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAELNPKITDFGMIKLCSINR 360
           DW  RY+II G++RGL+YLH DSRFK++H D+K SN+LLD  +NPKI DFGM KL   ++
Sbjct: 440 DWETRYRIISGVARGLLYLHEDSRFKIVHRDMKASNVLLDDAMNPKIADFGMAKLFDTDQ 499

Query: 361 SHGD--TSKIKGTYGYMAQ----------------------KIVSGQKNSCFQDGENIEH 420
           +     TSK+ GTYGYMA                       +I+ G+KN+   + ++   
Sbjct: 500 TSQTRFTSKVAGTYGYMAPEYAMSGEFSVKTDVFSFGVLVLEIIKGKKNNWSPEEDSSLF 559

Query: 421 LLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPILATT--CIDEIVRCIH 458
           LLSYV                       WK+W EG V+ I+DP L  T    DEI++CIH
Sbjct: 560 LLSYV-----------------------WKSWREGEVLNIVDPSLVETIGVSDEIMKCIH 619

BLAST of Bhi03M000400 vs. ExPASy Swiss-Prot
Match: Q9M0X5 (Cysteine-rich receptor-like protein kinase 25 OS=Arabidopsis thaliana OX=3702 GN=CRK25 PE=3 SV=1)

HSP 1 Score: 298.9 bits (764), Expect = 1.0e-79
Identity = 186/537 (34.64%), Postives = 282/537 (52.51%), Query Frame = 0

Query: 1   MLRYSSRPIFGAMEDEP-VFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLKYA 60
           M+RYS++ I G M   P VF+   +N T  Q   F ++L ALL  +   A+   SS K+A
Sbjct: 126 MVRYSNQSIVGQMRIRPGVFLTNKQNITENQVSRFNESLPALLIDVAVKAAL--SSRKFA 185

Query: 61  AGDIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKT--WRYYSPSCNVRYQ 120
                   F  IY+L+QCTPDL+   C++ L      +P CC ++   R  +PSC+ RY+
Sbjct: 186 TEKANFTVFQTIYSLVQCTPDLTNQDCESCLRQVINYLPRCCDRSVGGRVIAPSCSFRYE 245

Query: 121 MSLFFD---PSADLPPPP------PLIFVPS----------------------------- 180
           +  F++    +A + PPP      P + +PS                             
Sbjct: 246 LYPFYNETIAAAPMAPPPSSTVTAPPLNIPSEKGKGKNLTVIVTAIAVPVSVCVLLLGAM 305

Query: 181 --------SNSRSSEAPNI------SSYNYHFEM-------------------------- 240
                   +N  S+E  ++      S+    F+                           
Sbjct: 306 CWLLARRRNNKLSAETEDLDEDGITSTETLQFQFSAIEAATNKFSESNKLGHGGFGEVYK 365

Query: 241 GKLFNGQEVAVKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFL 300
           G+L  G+ VA+KRL+QGS QG  EFKNEV +VAKLQHRNL +LLG+C +  E++L+YEF+
Sbjct: 366 GQLITGETVAIKRLSQGSTQGAEEFKNEVDVVAKLQHRNLAKLLGYCLDGEEKILVYEFV 425

Query: 301 LNSSLDHFIFDPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAE 360
            N SLD+F+FD  +R  LDW RRYKII GI+RG++YLH DSR  +IH DLK SNILLDA+
Sbjct: 426 PNKSLDYFLFDNEKRRVLDWQRRYKIIEGIARGILYLHRDSRLTIIHRDLKASNILLDAD 485

Query: 361 LNPKITDFGMIKLCSINRSHGDTSKIKGTYGYMAQ----------------------KIV 420
           ++PKI+DFGM ++  ++++  +T +I GTYGYM+                       +++
Sbjct: 486 MHPKISDFGMARIFGVDQTQANTKRIVGTYGYMSPEYAIHGKYSVKSDVYSFGVLVLELI 545

Query: 421 SGQKNSCFQDGENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPI 434
           +G+KNS F + + +  L++YV                       WK W+E + + ++D  
Sbjct: 546 TGKKNSSFYEEDGLGDLVTYV-----------------------WKLWVENSPLELVDEA 605

BLAST of Bhi03M000400 vs. ExPASy Swiss-Prot
Match: Q8GYA4 (Cysteine-rich receptor-like protein kinase 10 OS=Arabidopsis thaliana OX=3702 GN=CRK10 PE=1 SV=3)

HSP 1 Score: 298.5 bits (763), Expect = 1.3e-79
Identity = 198/559 (35.42%), Postives = 274/559 (49.02%), Query Frame = 0

Query: 1   MLRYSSRPIFGAM-EDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSG-GSSLKY 60
           +LRYS++ I   +     V ++  +N T  Q DL       +L  L   A+    SS K+
Sbjct: 134 VLRYSNQNILSTLITTGGVILVNTRNVTSNQLDLLSD---LVLPTLNQAATVALNSSKKF 193

Query: 61  AAGDIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKTWRYYSPSCNVRYQM 120
                   A    Y L+QCTPDL++  C   L      IP+      R  +PSC  RY++
Sbjct: 194 GTRKNNFTALQSFYGLVQCTPDLTRQDCSRCLQLVINQIPTDRIGA-RIINPSCTSRYEI 253

Query: 121 SLFFDPSA-DLPPPPPLIFVP--SSNSRSSEAPN-------------------ISSYNY- 180
             F+  SA   PPPPP I  P  S+  RS +  N                   I+ Y + 
Sbjct: 254 YAFYTESAVPPPPPPPSISTPPVSAPPRSGKDGNSKVLVIAIVVPIIVAVLLFIAGYCFL 313

Query: 181 ---------------------------------------------------HFEMGKLFN 240
                                                                  G L +
Sbjct: 314 TRRARKSYYTPSAFAGDDITTADSLQLDYRTIQTATDDFVESNKIGQGGFGEVYKGTLSD 373

Query: 241 GQEVAVKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLLNSSL 300
           G EVAVKRL++ S QG+ EFKNEV+LVAKLQHRNLVRLLGFC +  ER+L+YE++ N SL
Sbjct: 374 GTEVAVKRLSKSSGQGEVEFKNEVVLVAKLQHRNLVRLLGFCLDGEERVLVYEYVPNKSL 433

Query: 301 DHFIFDPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAELNPKI 360
           D+F+FDP ++  LDW+RRYKII G++RG++YLH DSR  +IH DLK SNILLDA++NPKI
Sbjct: 434 DYFLFDPAKKGQLDWTRRYKIIGGVARGILYLHQDSRLTIIHRDLKASNILLDADMNPKI 493

Query: 361 TDFGMIKLCSINRSHGDTSKIKGTYGYMAQ----------------------KIVSGQKN 420
            DFGM ++  ++++  +TS+I GTYGYM+                       +I+SG+KN
Sbjct: 494 ADFGMARIFGLDQTEENTSRIVGTYGYMSPEYAMHGQYSMKSDVYSFGVLVLEIISGKKN 553

Query: 421 SCFQDGENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPILATTC 461
           S F   +    L+SY                        W  W  G  + ++DP +   C
Sbjct: 554 SSFYQTDGAHDLVSY-----------------------AWGLWSNGRPLELVDPAIVENC 613

BLAST of Bhi03M000400 vs. NCBI nr
Match: XP_038883071.1 (cysteine-rich receptor-like protein kinase 29 [Benincasa hispida])

HSP 1 Score: 636.3 bits (1640), Expect = 2.0e-178
Identity = 347/558 (62.19%), Postives = 382/558 (68.46%), Query Frame = 0

Query: 1   MLRYSSRPIFGAMEDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLKYAA 60
           MLR+SSRPIFGAME EP ++ WI ND   Q D+F+QNL  LLD LKDNASSGGS  KYAA
Sbjct: 117 MLRFSSRPIFGAMEVEPQYVSWITNDFKTQRDMFKQNLRTLLDRLKDNASSGGSVRKYAA 176

Query: 61  GDIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKTWRYYSPSCNVRYQMSL 120
           GDIFGEAFGRIYALLQCTPDLSKLQCDA  DSAF+TIP CC   WRY+SPSCNVRYQ+S 
Sbjct: 177 GDIFGEAFGRIYALLQCTPDLSKLQCDACSDSAFKTIPPCCKVAWRYFSPSCNVRYQVSH 236

Query: 121 FFDPSADLPPPPPLIFVPSSNSRSSEA--------------------------------- 180
           F +PSA+LPPP PLI VPSS + SSE                                  
Sbjct: 237 FLEPSAELPPPLPLILVPSSITTSSEGMKKNAMQTTIVIVLPFVALFVLAIGIYTFLRSK 296

Query: 181 ---------------PNISSYNY--------HFEM--------------GKLFNGQEVAV 240
                           N+  Y++        +F +              GKLFNGQE+AV
Sbjct: 297 KLREKLETSDDDISPTNLLQYDFKTIRDATDNFSVSNKLGQGGFGIVYKGKLFNGQEIAV 356

Query: 241 KRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLLNSSLDHFIFD 300
           KRL QGSQQGD EFKNEVLLVAKLQHRN+VRLLGFCFERNERLLIYEFL NSSLD FIFD
Sbjct: 357 KRLTQGSQQGDLEFKNEVLLVAKLQHRNVVRLLGFCFERNERLLIYEFLSNSSLDRFIFD 416

Query: 301 PIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAELNPKITDFGMI 360
           PIQR YLDWSRRYKI+VGISRGL+YLH DSRFKVIHHDLK SNILLDAELNPK+TDFGM 
Sbjct: 417 PIQREYLDWSRRYKIVVGISRGLMYLHEDSRFKVIHHDLKASNILLDAELNPKVTDFGMA 476

Query: 361 KLCSINRSHGDTSKIKGTYGYMAQ----------------------KIVSGQKNSCFQDG 420
           +LCSI+++HGDTSKIKGTYGYMA                       +I+SGQKNSCFQ+G
Sbjct: 477 RLCSISQTHGDTSKIKGTYGYMAPEYALYGRFSVKSDVFSFGVLLLEIISGQKNSCFQEG 536

Query: 421 ENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPILATTCIDEIVR 466
           EN EHLLSY                       TW+NWMEGNV AIIDP LA TCIDEI+R
Sbjct: 537 ENTEHLLSY-----------------------TWRNWMEGNVTAIIDPTLARTCIDEIIR 596

BLAST of Bhi03M000400 vs. NCBI nr
Match: XP_022950182.1 (cysteine-rich receptor-like protein kinase 29 isoform X2 [Cucurbita moschata])

HSP 1 Score: 618.6 bits (1594), Expect = 4.4e-173
Identity = 342/560 (61.07%), Postives = 379/560 (67.68%), Query Frame = 0

Query: 1   MLRYSSRPIFGAMEDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLKYAA 60
           MLRYSSR +FG ME EP++I+ + ND++ +EDLF+ NL  LL  LKDNASSG S  KYAA
Sbjct: 90  MLRYSSRRMFGTMEAEPMYIVLMNNDSVIKEDLFKHNLRTLLYSLKDNASSGDSLRKYAA 149

Query: 61  GDIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKTWRYYSPSCNVRYQMSL 120
           GDIFGE  GRIYAL+QCTPDLS+++CDA LDSA Q IP+CCS  WR +SPSCNVRYQMS 
Sbjct: 150 GDIFGEGLGRIYALMQCTPDLSQIECDACLDSAIQKIPACCSVAWRLFSPSCNVRYQMSP 209

Query: 121 FFDPSADLPPPPPLIFVPSSNSRSSE---------------------------------- 180
           FFDP   + PP PL+FVPSSN+ SSE                                  
Sbjct: 210 FFDPLL-VSPPLPLVFVPSSNTTSSEDKKKTAVHTTIVIALPFVVVSVFAIGIYMFLRSR 269

Query: 181 ----------APNISSYN---YHFE------------------------MGKLFNGQEVA 240
                       +IS  N   Y F+                         GKL NGQEVA
Sbjct: 270 KLRERVETTDHDDISPVNFLQYDFKTIRDATDDFAISNKLGQGGFGAVYKGKLLNGQEVA 329

Query: 241 VKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLLNSSLDHFIF 300
           VKRLAQGSQQGDFEFK EV LVAKLQHRNLVRLLGFCFERNERLLIYE LLNSSLD FIF
Sbjct: 330 VKRLAQGSQQGDFEFKTEVSLVAKLQHRNLVRLLGFCFERNERLLIYELLLNSSLDRFIF 389

Query: 301 DPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAELNPKITDFGM 360
           DP+QR YLDWSRRY+IIVGISRGLVYLH DSRFKVIHHDLK SNILLDA+LNPKITDFGM
Sbjct: 390 DPVQRQYLDWSRRYRIIVGISRGLVYLHEDSRFKVIHHDLKASNILLDADLNPKITDFGM 449

Query: 361 IKLCSINRSHGDTSKIKGTYGYMAQ----------------------KIVSGQKNSCFQD 420
            +LCS ++S GDTSKIKGTYGYMA                       +I+SGQKNSCFQD
Sbjct: 450 ARLCSTDQSDGDTSKIKGTYGYMAPEYALYGHFSVKSDVFSFGVILLEIMSGQKNSCFQD 509

Query: 421 GENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPILATTCIDEIV 467
           GEN EHLLSY                       TWKNWMEGNV AIIDPIL  TC+DE+V
Sbjct: 510 GENTEHLLSY-----------------------TWKNWMEGNVGAIIDPILGRTCMDEMV 569

BLAST of Bhi03M000400 vs. NCBI nr
Match: XP_022950181.1 (cysteine-rich receptor-like protein kinase 26 isoform X1 [Cucurbita moschata])

HSP 1 Score: 618.6 bits (1594), Expect = 4.4e-173
Identity = 342/560 (61.07%), Postives = 379/560 (67.68%), Query Frame = 0

Query: 1   MLRYSSRPIFGAMEDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLKYAA 60
           MLRYSSR +FG ME EP++I+ + ND++ +EDLF+ NL  LL  LKDNASSG S  KYAA
Sbjct: 126 MLRYSSRRMFGTMEAEPMYIVLMNNDSVIKEDLFKHNLRTLLYSLKDNASSGDSLRKYAA 185

Query: 61  GDIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKTWRYYSPSCNVRYQMSL 120
           GDIFGE  GRIYAL+QCTPDLS+++CDA LDSA Q IP+CCS  WR +SPSCNVRYQMS 
Sbjct: 186 GDIFGEGLGRIYALMQCTPDLSQIECDACLDSAIQKIPACCSVAWRLFSPSCNVRYQMSP 245

Query: 121 FFDPSADLPPPPPLIFVPSSNSRSSE---------------------------------- 180
           FFDP   + PP PL+FVPSSN+ SSE                                  
Sbjct: 246 FFDPLL-VSPPLPLVFVPSSNTTSSEDKKKTAVHTTIVIALPFVVVSVFAIGIYMFLRSR 305

Query: 181 ----------APNISSYN---YHFE------------------------MGKLFNGQEVA 240
                       +IS  N   Y F+                         GKL NGQEVA
Sbjct: 306 KLRERVETTDHDDISPVNFLQYDFKTIRDATDDFAISNKLGQGGFGAVYKGKLLNGQEVA 365

Query: 241 VKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLLNSSLDHFIF 300
           VKRLAQGSQQGDFEFK EV LVAKLQHRNLVRLLGFCFERNERLLIYE LLNSSLD FIF
Sbjct: 366 VKRLAQGSQQGDFEFKTEVSLVAKLQHRNLVRLLGFCFERNERLLIYELLLNSSLDRFIF 425

Query: 301 DPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAELNPKITDFGM 360
           DP+QR YLDWSRRY+IIVGISRGLVYLH DSRFKVIHHDLK SNILLDA+LNPKITDFGM
Sbjct: 426 DPVQRQYLDWSRRYRIIVGISRGLVYLHEDSRFKVIHHDLKASNILLDADLNPKITDFGM 485

Query: 361 IKLCSINRSHGDTSKIKGTYGYMAQ----------------------KIVSGQKNSCFQD 420
            +LCS ++S GDTSKIKGTYGYMA                       +I+SGQKNSCFQD
Sbjct: 486 ARLCSTDQSDGDTSKIKGTYGYMAPEYALYGHFSVKSDVFSFGVILLEIMSGQKNSCFQD 545

Query: 421 GENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPILATTCIDEIV 467
           GEN EHLLSY                       TWKNWMEGNV AIIDPIL  TC+DE+V
Sbjct: 546 GENTEHLLSY-----------------------TWKNWMEGNVGAIIDPILGRTCMDEMV 605

BLAST of Bhi03M000400 vs. NCBI nr
Match: XP_023544482.1 (cysteine-rich receptor-like protein kinase 29 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 616.7 bits (1589), Expect = 1.7e-172
Identity = 342/560 (61.07%), Postives = 376/560 (67.14%), Query Frame = 0

Query: 1   MLRYSSRPIFGAMEDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLKYAA 60
           MLRYSSR +FG ME EP++I+ + ND++ +EDLF+ NL  LL  LKD+ASSG S  KYAA
Sbjct: 117 MLRYSSRRMFGTMEAEPMYIVLMNNDSVIKEDLFKHNLRTLLYSLKDDASSGDSLRKYAA 176

Query: 61  GDIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKTWRYYSPSCNVRYQMSL 120
           GDIFGE  GRIYALLQCTPDLS+++CDA LDSA Q IP+CCS  WR +SPSCNVRYQMS 
Sbjct: 177 GDIFGEGLGRIYALLQCTPDLSQIECDACLDSAIQKIPACCSVAWRLFSPSCNVRYQMSP 236

Query: 121 FFDPSADLPPPPPLIFVPSSNSRSSEAPN------------------------------- 180
           FFD +    PP PL+FVPSSN+ SSE                                  
Sbjct: 237 FFD-TLLASPPLPLVFVPSSNTMSSEDKKKNVVHTTIVIALPFVVVSVFTIGIYMFLRSR 296

Query: 181 ----------------ISSYNYHFE------------------------MGKLFNGQEVA 240
                           ++   Y F+                         GKL NGQEVA
Sbjct: 297 KLRGRVETTDHDEISPVNFLQYDFKTIRDATDDFAISNKLGQGGFGAVYKGKLLNGQEVA 356

Query: 241 VKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLLNSSLDHFIF 300
           VKRLAQGSQQGDFEFK EV LVAKLQHRNLVRLLGFCFERNERLLIYE LLNSSLD FIF
Sbjct: 357 VKRLAQGSQQGDFEFKTEVSLVAKLQHRNLVRLLGFCFERNERLLIYELLLNSSLDRFIF 416

Query: 301 DPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAELNPKITDFGM 360
           DP+QR YLDWSRRY+IIVGISRGLVYLH DSRFKVIHHDLK SNILLDAELNPKITDFGM
Sbjct: 417 DPVQRQYLDWSRRYRIIVGISRGLVYLHEDSRFKVIHHDLKASNILLDAELNPKITDFGM 476

Query: 361 IKLCSINRSHGDTSKIKGTYGYMAQ----------------------KIVSGQKNSCFQD 420
            +LCS ++S GDTSKIKGTYGYMA                       +I+SGQKNSCFQD
Sbjct: 477 ARLCSTDQSDGDTSKIKGTYGYMAPEYALYGHFSVKSDVFSFGVILLEIMSGQKNSCFQD 536

Query: 421 GENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPILATTCIDEIV 467
           GEN EHLLSY                       TWKNWMEGNV AIIDPIL  TC+DE+V
Sbjct: 537 GENTEHLLSY-----------------------TWKNWMEGNVGAIIDPILGRTCMDEMV 596

BLAST of Bhi03M000400 vs. NCBI nr
Match: XP_022142346.1 (cysteine-rich receptor-like protein kinase 29 [Momordica charantia])

HSP 1 Score: 592.8 bits (1527), Expect = 2.6e-165
Identity = 337/565 (59.65%), Postives = 368/565 (65.13%), Query Frame = 0

Query: 1   MLRYSSRPIFGAMEDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLKYAA 60
           MLRYS+RPIFGAME +P  +M + ++ +AQ+DLF QNL  L DGLK NASSGGS  KYAA
Sbjct: 117 MLRYSNRPIFGAMEVDPKLLMLLPSNNVAQDDLFSQNLRTLFDGLKKNASSGGSLKKYAA 176

Query: 61  GDIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKTWRYYSPSCNVRYQMSL 120
           G I G   GRIYALLQCTPDLS+ QCD  LD+AF+  P CC K  R + PSCN RY  S 
Sbjct: 177 GYILGPGLGRIYALLQCTPDLSQSQCDVCLDTAFKATPPCCRKGLRSFLPSCNFRYDTSR 236

Query: 121 FFDPSADLPPPPPLIFVPSSNSRSSEA--------------------------------- 180
           FFD S D  PPP L+F PS+++ S+E                                  
Sbjct: 237 FFDLS-DYSPPPALVFTPSTHTISTEGMANGKKNNTKKMVIIVVLPFTALLVLATGIYAF 296

Query: 181 --------------PNISSYN---YHFE------------------------MGKLFNGQ 240
                           IS  N   Y F+                         GKLFNG 
Sbjct: 297 LRSRKLRERLETSDDEISPINLLQYDFKTIRDATNDFSISNKLGQGGFGLVYKGKLFNGL 356

Query: 241 EVAVKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLLNSSLDH 300
           EVAVKRLA+GSQQGD EFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFL+NSSLD 
Sbjct: 357 EVAVKRLARGSQQGDSEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLVNSSLDR 416

Query: 301 FIFDPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAELNPKITD 360
           FIFDPIQR YLDW RRYKIIVGISRGL+YLH DSRFKVIH DLK+SNILLDAELNPKITD
Sbjct: 417 FIFDPIQRQYLDWLRRYKIIVGISRGLMYLHEDSRFKVIHQDLKSSNILLDAELNPKITD 476

Query: 361 FGMIKLCSINRSHGDTSKIKGTYGYMAQ----------------------KIVSGQKNSC 420
           FGM +LCSIN+SHGDTS+IKGTYGYMA                       +I+SGQKNSC
Sbjct: 477 FGMARLCSINQSHGDTSRIKGTYGYMAPEYALYGHFSVKSDVFSFGVLLLEIISGQKNSC 536

Query: 421 FQDGENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPILATTCID 469
           FQDGEN EHLL+Y                       TWKNWMEG V  IIDPILA TCID
Sbjct: 537 FQDGENTEHLLTY-----------------------TWKNWMEGTVTNIIDPILARTCID 596

BLAST of Bhi03M000400 vs. ExPASy TrEMBL
Match: A0A6J1GE39 (cysteine-rich receptor-like protein kinase 26 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111453352 PE=4 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 2.1e-173
Identity = 342/560 (61.07%), Postives = 379/560 (67.68%), Query Frame = 0

Query: 1   MLRYSSRPIFGAMEDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLKYAA 60
           MLRYSSR +FG ME EP++I+ + ND++ +EDLF+ NL  LL  LKDNASSG S  KYAA
Sbjct: 126 MLRYSSRRMFGTMEAEPMYIVLMNNDSVIKEDLFKHNLRTLLYSLKDNASSGDSLRKYAA 185

Query: 61  GDIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKTWRYYSPSCNVRYQMSL 120
           GDIFGE  GRIYAL+QCTPDLS+++CDA LDSA Q IP+CCS  WR +SPSCNVRYQMS 
Sbjct: 186 GDIFGEGLGRIYALMQCTPDLSQIECDACLDSAIQKIPACCSVAWRLFSPSCNVRYQMSP 245

Query: 121 FFDPSADLPPPPPLIFVPSSNSRSSE---------------------------------- 180
           FFDP   + PP PL+FVPSSN+ SSE                                  
Sbjct: 246 FFDPLL-VSPPLPLVFVPSSNTTSSEDKKKTAVHTTIVIALPFVVVSVFAIGIYMFLRSR 305

Query: 181 ----------APNISSYN---YHFE------------------------MGKLFNGQEVA 240
                       +IS  N   Y F+                         GKL NGQEVA
Sbjct: 306 KLRERVETTDHDDISPVNFLQYDFKTIRDATDDFAISNKLGQGGFGAVYKGKLLNGQEVA 365

Query: 241 VKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLLNSSLDHFIF 300
           VKRLAQGSQQGDFEFK EV LVAKLQHRNLVRLLGFCFERNERLLIYE LLNSSLD FIF
Sbjct: 366 VKRLAQGSQQGDFEFKTEVSLVAKLQHRNLVRLLGFCFERNERLLIYELLLNSSLDRFIF 425

Query: 301 DPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAELNPKITDFGM 360
           DP+QR YLDWSRRY+IIVGISRGLVYLH DSRFKVIHHDLK SNILLDA+LNPKITDFGM
Sbjct: 426 DPVQRQYLDWSRRYRIIVGISRGLVYLHEDSRFKVIHHDLKASNILLDADLNPKITDFGM 485

Query: 361 IKLCSINRSHGDTSKIKGTYGYMAQ----------------------KIVSGQKNSCFQD 420
            +LCS ++S GDTSKIKGTYGYMA                       +I+SGQKNSCFQD
Sbjct: 486 ARLCSTDQSDGDTSKIKGTYGYMAPEYALYGHFSVKSDVFSFGVILLEIMSGQKNSCFQD 545

Query: 421 GENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPILATTCIDEIV 467
           GEN EHLLSY                       TWKNWMEGNV AIIDPIL  TC+DE+V
Sbjct: 546 GENTEHLLSY-----------------------TWKNWMEGNVGAIIDPILGRTCMDEMV 605

BLAST of Bhi03M000400 vs. ExPASy TrEMBL
Match: A0A6J1GE34 (cysteine-rich receptor-like protein kinase 29 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111453352 PE=4 SV=1)

HSP 1 Score: 618.6 bits (1594), Expect = 2.1e-173
Identity = 342/560 (61.07%), Postives = 379/560 (67.68%), Query Frame = 0

Query: 1   MLRYSSRPIFGAMEDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLKYAA 60
           MLRYSSR +FG ME EP++I+ + ND++ +EDLF+ NL  LL  LKDNASSG S  KYAA
Sbjct: 90  MLRYSSRRMFGTMEAEPMYIVLMNNDSVIKEDLFKHNLRTLLYSLKDNASSGDSLRKYAA 149

Query: 61  GDIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKTWRYYSPSCNVRYQMSL 120
           GDIFGE  GRIYAL+QCTPDLS+++CDA LDSA Q IP+CCS  WR +SPSCNVRYQMS 
Sbjct: 150 GDIFGEGLGRIYALMQCTPDLSQIECDACLDSAIQKIPACCSVAWRLFSPSCNVRYQMSP 209

Query: 121 FFDPSADLPPPPPLIFVPSSNSRSSE---------------------------------- 180
           FFDP   + PP PL+FVPSSN+ SSE                                  
Sbjct: 210 FFDPLL-VSPPLPLVFVPSSNTTSSEDKKKTAVHTTIVIALPFVVVSVFAIGIYMFLRSR 269

Query: 181 ----------APNISSYN---YHFE------------------------MGKLFNGQEVA 240
                       +IS  N   Y F+                         GKL NGQEVA
Sbjct: 270 KLRERVETTDHDDISPVNFLQYDFKTIRDATDDFAISNKLGQGGFGAVYKGKLLNGQEVA 329

Query: 241 VKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLLNSSLDHFIF 300
           VKRLAQGSQQGDFEFK EV LVAKLQHRNLVRLLGFCFERNERLLIYE LLNSSLD FIF
Sbjct: 330 VKRLAQGSQQGDFEFKTEVSLVAKLQHRNLVRLLGFCFERNERLLIYELLLNSSLDRFIF 389

Query: 301 DPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAELNPKITDFGM 360
           DP+QR YLDWSRRY+IIVGISRGLVYLH DSRFKVIHHDLK SNILLDA+LNPKITDFGM
Sbjct: 390 DPVQRQYLDWSRRYRIIVGISRGLVYLHEDSRFKVIHHDLKASNILLDADLNPKITDFGM 449

Query: 361 IKLCSINRSHGDTSKIKGTYGYMAQ----------------------KIVSGQKNSCFQD 420
            +LCS ++S GDTSKIKGTYGYMA                       +I+SGQKNSCFQD
Sbjct: 450 ARLCSTDQSDGDTSKIKGTYGYMAPEYALYGHFSVKSDVFSFGVILLEIMSGQKNSCFQD 509

Query: 421 GENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPILATTCIDEIV 467
           GEN EHLLSY                       TWKNWMEGNV AIIDPIL  TC+DE+V
Sbjct: 510 GENTEHLLSY-----------------------TWKNWMEGNVGAIIDPILGRTCMDEMV 569

BLAST of Bhi03M000400 vs. ExPASy TrEMBL
Match: A0A6J1CLX3 (cysteine-rich receptor-like protein kinase 29 OS=Momordica charantia OX=3673 GN=LOC111012489 PE=4 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 1.3e-165
Identity = 337/565 (59.65%), Postives = 368/565 (65.13%), Query Frame = 0

Query: 1   MLRYSSRPIFGAMEDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLKYAA 60
           MLRYS+RPIFGAME +P  +M + ++ +AQ+DLF QNL  L DGLK NASSGGS  KYAA
Sbjct: 117 MLRYSNRPIFGAMEVDPKLLMLLPSNNVAQDDLFSQNLRTLFDGLKKNASSGGSLKKYAA 176

Query: 61  GDIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKTWRYYSPSCNVRYQMSL 120
           G I G   GRIYALLQCTPDLS+ QCD  LD+AF+  P CC K  R + PSCN RY  S 
Sbjct: 177 GYILGPGLGRIYALLQCTPDLSQSQCDVCLDTAFKATPPCCRKGLRSFLPSCNFRYDTSR 236

Query: 121 FFDPSADLPPPPPLIFVPSSNSRSSEA--------------------------------- 180
           FFD S D  PPP L+F PS+++ S+E                                  
Sbjct: 237 FFDLS-DYSPPPALVFTPSTHTISTEGMANGKKNNTKKMVIIVVLPFTALLVLATGIYAF 296

Query: 181 --------------PNISSYN---YHFE------------------------MGKLFNGQ 240
                           IS  N   Y F+                         GKLFNG 
Sbjct: 297 LRSRKLRERLETSDDEISPINLLQYDFKTIRDATNDFSISNKLGQGGFGLVYKGKLFNGL 356

Query: 241 EVAVKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLLNSSLDH 300
           EVAVKRLA+GSQQGD EFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFL+NSSLD 
Sbjct: 357 EVAVKRLARGSQQGDSEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLVNSSLDR 416

Query: 301 FIFDPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAELNPKITD 360
           FIFDPIQR YLDW RRYKIIVGISRGL+YLH DSRFKVIH DLK+SNILLDAELNPKITD
Sbjct: 417 FIFDPIQRQYLDWLRRYKIIVGISRGLMYLHEDSRFKVIHQDLKSSNILLDAELNPKITD 476

Query: 361 FGMIKLCSINRSHGDTSKIKGTYGYMAQ----------------------KIVSGQKNSC 420
           FGM +LCSIN+SHGDTS+IKGTYGYMA                       +I+SGQKNSC
Sbjct: 477 FGMARLCSINQSHGDTSRIKGTYGYMAPEYALYGHFSVKSDVFSFGVLLLEIISGQKNSC 536

Query: 421 FQDGENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPILATTCID 469
           FQDGEN EHLL+Y                       TWKNWMEG V  IIDPILA TCID
Sbjct: 537 FQDGENTEHLLTY-----------------------TWKNWMEGTVTNIIDPILARTCID 596

BLAST of Bhi03M000400 vs. ExPASy TrEMBL
Match: A0A6J1CLB6 (cysteine-rich receptor-like protein kinase 29 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012490 PE=4 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 8.2e-149
Identity = 304/558 (54.48%), Postives = 348/558 (62.37%), Query Frame = 0

Query: 1   MLRYSSRPIFGAMEDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLKYAA 60
           MLRYSSR I GAME  P F +W   D + +E LF Q L  LLD L+  ASSGG   KYA 
Sbjct: 90  MLRYSSRHILGAMEIGPSFFIW-ATDNVTEEVLFEQKLVTLLDSLRSRASSGGYLQKYAT 149

Query: 61  GDIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKTWRYYSPSCNVRYQMSL 120
           GD+FG+  G IY LLQCTPDLS+LQC+A LDSAF  IP CCSK  R +SPSC+VRY+MSL
Sbjct: 150 GDVFGQGLGTIYGLLQCTPDLSQLQCEACLDSAFTLIPPCCSKGVRVFSPSCDVRYEMSL 209

Query: 121 FFDPSADLP----PPPPLIFVPSSNSRSS------------------------------- 180
           FF+PS+D P    PPP L+F PS+++ S+                               
Sbjct: 210 FFEPSSDSPPPLLPPPTLVFTPSTSTTSTKGKMNNTVQTVIVIVLPFVALLVLLTISIYT 269

Query: 181 ----------------EAPNISSYNYHFE------------------------MGKLFNG 240
                           +  ++ S  Y F+                         GK  NG
Sbjct: 270 FLNSRKLRERLETSDDDTSSVGSLQYDFKTIRDATDNFSTANKLGEGGFGIVYKGKFLNG 329

Query: 241 QEVAVKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLLNSSLD 300
           Q+VAVKRLA+GSQQGD EFKNEVLLVA LQHRNLVRLLGFCFER+ERLLIYEFL NSSLD
Sbjct: 330 QDVAVKRLARGSQQGDSEFKNEVLLVANLQHRNLVRLLGFCFERSERLLIYEFLPNSSLD 389

Query: 301 HFIFDPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAELNPKIT 360
             IFDPIQR +LDWSRRYKIIVGISRGL+YLH DS+F+VIHHDLK SNILLDAELNPKIT
Sbjct: 390 RLIFDPIQRKHLDWSRRYKIIVGISRGLLYLHEDSQFRVIHHDLKASNILLDAELNPKIT 449

Query: 361 DFGMIKLCSINRSH--GDTSKIKGTYGYMAQ----------------------KIVSGQK 420
           DFG+ +LCS N+S   GDTSKI+GTYGYMA                       +I+SGQK
Sbjct: 450 DFGIARLCSFNQSKSVGDTSKIRGTYGYMAPEYALYGHFSVRSDVYSFGVLVLEILSGQK 509

Query: 421 NSCFQDGENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPILATT 460
           NSCFQDGENIE LLSY                        WKNW EG +  IIDP+LA T
Sbjct: 510 NSCFQDGENIEDLLSY-----------------------AWKNWTEGTITNIIDPLLAGT 569

BLAST of Bhi03M000400 vs. ExPASy TrEMBL
Match: A0A6J1CN19 (cysteine-rich receptor-like protein kinase 26 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012490 PE=4 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 8.2e-149
Identity = 304/558 (54.48%), Postives = 348/558 (62.37%), Query Frame = 0

Query: 1   MLRYSSRPIFGAMEDEPVFIMWIKNDTIAQEDLFRQNLGALLDGLKDNASSGGSSLKYAA 60
           MLRYSSR I GAME  P F +W   D + +E LF Q L  LLD L+  ASSGG   KYA 
Sbjct: 117 MLRYSSRHILGAMEIGPSFFIW-ATDNVTEEVLFEQKLVTLLDSLRSRASSGGYLQKYAT 176

Query: 61  GDIFGEAFGRIYALLQCTPDLSKLQCDAYLDSAFQTIPSCCSKTWRYYSPSCNVRYQMSL 120
           GD+FG+  G IY LLQCTPDLS+LQC+A LDSAF  IP CCSK  R +SPSC+VRY+MSL
Sbjct: 177 GDVFGQGLGTIYGLLQCTPDLSQLQCEACLDSAFTLIPPCCSKGVRVFSPSCDVRYEMSL 236

Query: 121 FFDPSADLP----PPPPLIFVPSSNSRSS------------------------------- 180
           FF+PS+D P    PPP L+F PS+++ S+                               
Sbjct: 237 FFEPSSDSPPPLLPPPTLVFTPSTSTTSTKGKMNNTVQTVIVIVLPFVALLVLLTISIYT 296

Query: 181 ----------------EAPNISSYNYHFE------------------------MGKLFNG 240
                           +  ++ S  Y F+                         GK  NG
Sbjct: 297 FLNSRKLRERLETSDDDTSSVGSLQYDFKTIRDATDNFSTANKLGEGGFGIVYKGKFLNG 356

Query: 241 QEVAVKRLAQGSQQGDFEFKNEVLLVAKLQHRNLVRLLGFCFERNERLLIYEFLLNSSLD 300
           Q+VAVKRLA+GSQQGD EFKNEVLLVA LQHRNLVRLLGFCFER+ERLLIYEFL NSSLD
Sbjct: 357 QDVAVKRLARGSQQGDSEFKNEVLLVANLQHRNLVRLLGFCFERSERLLIYEFLPNSSLD 416

Query: 301 HFIFDPIQRHYLDWSRRYKIIVGISRGLVYLHGDSRFKVIHHDLKTSNILLDAELNPKIT 360
             IFDPIQR +LDWSRRYKIIVGISRGL+YLH DS+F+VIHHDLK SNILLDAELNPKIT
Sbjct: 417 RLIFDPIQRKHLDWSRRYKIIVGISRGLLYLHEDSQFRVIHHDLKASNILLDAELNPKIT 476

Query: 361 DFGMIKLCSINRSH--GDTSKIKGTYGYMAQ----------------------KIVSGQK 420
           DFG+ +LCS N+S   GDTSKI+GTYGYMA                       +I+SGQK
Sbjct: 477 DFGIARLCSFNQSKSVGDTSKIRGTYGYMAPEYALYGHFSVRSDVYSFGVLVLEILSGQK 536

Query: 421 NSCFQDGENIEHLLSYVSMNIFERYVLSLQSFNTNLPKQTWKNWMEGNVIAIIDPILATT 460
           NSCFQDGENIE LLSY                        WKNW EG +  IIDP+LA T
Sbjct: 537 NSCFQDGENIEDLLSY-----------------------AWKNWTEGTITNIIDPLLAGT 596

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT4G21410.13.1e-9240.34cysteine-rich RLK (RECEPTOR-like protein kinase) 29 [more]
AT4G38830.18.2e-8538.22cysteine-rich RLK (RECEPTOR-like protein kinase) 26 [more]
AT4G00970.12.9e-8237.18cysteine-rich RLK (RECEPTOR-like protein kinase) 41 [more]
AT4G05200.17.2e-8134.64cysteine-rich RLK (RECEPTOR-like protein kinase) 25 [more]
AT4G23180.19.4e-8135.42cysteine-rich RLK (RECEPTOR-like protein kinase) 10 [more]
Match NameE-valueIdentityDescription
Q8S9L64.4e-9140.34Cysteine-rich receptor-like protein kinase 29 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9T0J11.2e-8338.22Cysteine-rich receptor-like protein kinase 26 OS=Arabidopsis thaliana OX=3702 GN... [more]
O230814.1e-8137.18Cysteine-rich receptor-like protein kinase 41 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q9M0X51.0e-7934.64Cysteine-rich receptor-like protein kinase 25 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q8GYA41.3e-7935.42Cysteine-rich receptor-like protein kinase 10 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
XP_038883071.12.0e-17862.19cysteine-rich receptor-like protein kinase 29 [Benincasa hispida][more]
XP_022950182.14.4e-17361.07cysteine-rich receptor-like protein kinase 29 isoform X2 [Cucurbita moschata][more]
XP_022950181.14.4e-17361.07cysteine-rich receptor-like protein kinase 26 isoform X1 [Cucurbita moschata][more]
XP_023544482.11.7e-17261.07cysteine-rich receptor-like protein kinase 29 [Cucurbita pepo subsp. pepo][more]
XP_022142346.12.6e-16559.65cysteine-rich receptor-like protein kinase 29 [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1GE392.1e-17361.07cysteine-rich receptor-like protein kinase 26 isoform X1 OS=Cucurbita moschata O... [more]
A0A6J1GE342.1e-17361.07cysteine-rich receptor-like protein kinase 29 isoform X2 OS=Cucurbita moschata O... [more]
A0A6J1CLX31.3e-16559.65cysteine-rich receptor-like protein kinase 29 OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1CLB68.2e-14954.48cysteine-rich receptor-like protein kinase 29 isoform X2 OS=Momordica charantia ... [more]
A0A6J1CN198.2e-14954.48cysteine-rich receptor-like protein kinase 26 isoform X1 OS=Momordica charantia ... [more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 157..414
e-value: 5.5E-7
score: -35.9
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 164..321
e-value: 9.4E-27
score: 94.0
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 142..419
score: 21.248362
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 145..218
e-value: 4.5E-23
score: 83.3
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 220..422
e-value: 1.7E-32
score: 114.4
NoneNo IPR availablePANTHERPTHR27002:SF420CYSTEINE-RICH RECEPTOR-LIKE PROTEIN KINASE 10 ISOFORM X1coord: 160..314
coord: 1..134
coord: 316..439
NoneNo IPR availablePANTHERPTHR27002RECEPTOR-LIKE SERINE/THREONINE-PROTEIN KINASE SD1-8coord: 160..314
coord: 1..134
coord: 316..439
IPR038408Gnk2-homologous domain superfamilyGENE3D3.30.430.20coord: 17..121
e-value: 6.2E-21
score: 76.5
IPR002902Gnk2-homologous domainPFAMPF01657Stress-antifungcoord: 29..117
e-value: 3.5E-11
score: 43.6
IPR002902Gnk2-homologous domainPROSITEPS51473GNK2coord: 15..121
score: 15.112931
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 264..276
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 163..440

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Bhi03G000400Bhi03G000400gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi03M000400.exon7Bhi03M000400.exon7exon
Bhi03M000400.exon6Bhi03M000400.exon6exon
Bhi03M000400.exon5Bhi03M000400.exon5exon
Bhi03M000400.exon4Bhi03M000400.exon4exon
Bhi03M000400.exon3Bhi03M000400.exon3exon
Bhi03M000400.exon2Bhi03M000400.exon2exon
Bhi03M000400.exon1Bhi03M000400.exon1exon


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Bhi03M000400cds.Bhi03M000400_7CDS
cds.Bhi03M000400cds.Bhi03M000400_6CDS
cds.Bhi03M000400cds.Bhi03M000400_5CDS
cds.Bhi03M000400cds.Bhi03M000400_4CDS
cds.Bhi03M000400cds.Bhi03M000400_3CDS
cds.Bhi03M000400cds.Bhi03M000400_2CDS
cds.Bhi03M000400cds.Bhi03M000400CDS


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Bhi03M000400Bhi03M000400-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity