Bhi01M002368 (mRNA) Wax gourd (B227) v1

Overview
NameBhi01M002368
TypemRNA
OrganismBenincasa hispida (Wax gourd (B227) v1)
DescriptionPectinesterase
Locationchr1: 75600482 .. 75605077 (-)
Sequence length1449
RNA-Seq ExpressionBhi01M002368
SyntenyBhi01M002368
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTATTTTACACCCAAAACTTTCATTCTTCATATTATTTATACACTTTTCTTCTTGTATTCCCTTCTCCATACTCCACACATAGGACCAATGAATAATATTATGAAGTATAATCATGTCCTCACCAATCTTGCTTTATCATCTCTTCTCCCTCTCCTTGCTTTCCTCTTCATGATTAAGCCCACCCCTCCCTTCCTTTCCAATCTTGTCATCTTTCGCGACTCCATCGGCTGTCATCACCATCACCATCATCACCACCACCACCATCATCGCCGTCATCATAAAAACGCAGCCGACGAAGAAGACCAAGCAGCCATAAGTTCCATTTGTGATACCTTCCCGATCACCCTCCTCCCACCAGAAACCCCCAACACTTCTGTATTTTGTGTTGATCGAAACGGTTGTTGCAACTTCACGACTGTTCAAGCGGCAGTCGACGCTGTGCCGATCTTGAGCTTCAAACGAAACATAATTTGGATCAACATTGGAATTTACTAGTAAACTCAATATAAATACTAGCTATGGTTTTTTTTTTTTCCTTTTTGATAAAATGAAAATCCTTGACTTTCTCTCTTATTTCAAAAATATTTATATTATTCTTTTAAACATTACAATTTTTCTCTTAATCTTTTATAGATATTTGACCAAATTTTGAGATTTAAAACAGTGTGACAATCTTAAAATAAAAATTGGAATTTACGACTATGCTTGTTTCAAATACGCATTTTCATCCAAAAATATAATGAATCTTACTCTAATAATAGTTTTGTAAAACTTAAGAAAAGTTTCGATGACAAATCTGAAAGTTTTTTTTTTTTTTTTTTGAGTAATGTTGCAGTGAGAAAGTGATAGTTCCAAAATCTAAAGCTAATGTGACATTTCAAGGACAAGGGTATACAACAACTGCCATTGTTTGGAATGACACTGCCAATTCTTCCCATGGCACCTTTTATAGTGGCTCTGTTCAAGTCTTCTCTTCCAATTTTATTGCCAAGAATATTAGCTTCATGGTAATTTCTAATTATCAATATATATATCTTTCATTATCTATCTTTTAATGTTTGATATTCATTATATTACAAATTTACGTCTTTAGTTATTATTCGACTTTCTATTCTTCTTTTCTAAAAAATCAAGCTTATAAACATTATTTACGCCGGTTAATTTATTTGTTTTGTTATAATCTTTTCGTTCTTGTTTTTTAAAATCATTTAAAAATTAAGATTTCATTTGATAATCATTTCTTTTTTGGTTTTTGAATATTAAACCAATTTTCTCTCTATTTCTTACAATAATTTACATCTTTCTTAGGTACAATAGTTGAATTCTTAGCCAAATTCTATAAACAAAAACAATTTTTTAAAAGTTATTTTTTAGTTTTCAAAATTTGGGTAGTTTTTTAAACCATTAGTAAGAAGCAGATAACAAATGAAGAAATTTGGAGGTAAAAATAGTGCCTATAAACTTAATTTTTAAAAGCAAAAAATAAAAAACTACATGGTTACTAAACGATGCCTAAATAACTTTTCTCTTTAGAATTTAACTAAGAATTTAAATGTGTTTTTTTAAGAAGTGTGAAAACTATAGTATGGAATTTAGAAGGAAACAAGCAAAACTTTCAAAAATAGAAAATTAAAAATAAAATCATTACAAAAAAGATGTAATAATCCAACTTTATCAATTTAGTTTTTGAACTTTAAAAAGTGTTTGATAAGTCTTTAAATTTTTATTTTTATGTGGAAATTCAATTCCAAATCTAATAGGTATTTGACTTATTTGGTATTTTTTAAAAAAATATTCGCAAACTTATTTAGTCATTAAGTTGAAACTTTATTGTTCTATTATATATATATATATATATATATATATTTGTTATATACAACTTTAAAGTTTATGGACTTATTAGACCGTTCTTAACTTTAAAGATACTTTTATACACAATGTTTTACGCGTTTTTATGTTCTTTGGACCATTTTCTTAGACATATGATAACACGGGATTTAATGTCAGTTAGCAACCATGGTAGTTAACCATCATAATATCTATATAATATAAAAAATTTTGATGGTGGTTCAAAACTACCACAAAAAACAATCTGTCAACATATTTTATGATAAAAAACGATACAGTAACCATTTGTAATCAATCATCATACGTAATAATACCAATTCAAAATTAATATTTAAAGCCAAAATATCTGGAGTGTTTATTTTAATCTCTCTTTATTTTTATTTTTGTTTGTGCTTAGATGTTGTCCTTGTTATTCTTGAATTGTATGATAATATGAATTGACTTTTCTTTATTTTTCCAAACATCTTGAACTCATTTTTAGGCCGTGTAAACTCAAATAAATGGATAAATGACCTTTTAGTAAATATTTGGTTTTTTTGTCCTTTTCTTTTTAATTAAGCTTATAGACATTATTTTCACTCCCAAAATTTTTCCTTAATTATCTACTTTTTATCAATGGTTTAAAAGTCAAGCAAAAATTTGAAAACTAAAAAAAAAAAAGTTTTTGAAATTTTATTTTTGTTTTTGGAATTTAGCTAAGAATTCAATCATTGTGTTTGATAAACATATAAATCATTGTAAGAAATGAGTAAATAATGAGATTTTTAAAAATCAAAAATAAAAAACAAAATGATTATGAAAAAGGATAGTTATTTTATTGCTTGTTGATCAAATGAGAGATGCTTGACTTTGATTATATACATTAAATAAAAATTATTGTAAAAAATAGTTTACTTTTGTTATTATAAAAATATATAAAACTAAAAAATGTTATAAAAACTTTTTTTGATTTTTGAAAACAAAAAACAACAATTATCAAACATGTTTTTTCTTTCCCAACAAATAGAAAACATTCCAACAAAAACAGTACGTATAGTTTTTGTTTTTTAAAAACAGGAAATCATTTTAAAAAACCAAAAATCTAAAAATGTTAGGCCGAAAAAAAAAATTACGTTTAAATCTCAATTTTGCCCCTAAATTTTTCTAAGCAATTTTTATTGGGGCTTTGGCAGAATGTGGCGCCAATTCCAGGCCCGGGCGACGTCGGAGCACAGGCGGTGGCGATTAGAATCGGAGGAGACCAGGCCGCGTTTTGGAGCTGCGGATTCTTCGGTGCTCAAGACACTCTCCACGACGACCGTGGCCGCCATTACTTCAAAGATTGCTACATCCAAGGCTCCATCGATTTCATCTTCGGCAATGGAAGATCCTTCTACGAGGTAAATCGGAGCCCTAGATTTTTCGGCGAGTTCGATTTGACGATGAATTTCAGAATCGCGATTTTGACAACCTTGAAAACGCATTGTAGAGTTGCCAGTTGATTTCGATGGCGAATCCGGTTCCGCAAGGATCGAAGTTTGTGAACGGAGCGGTGACGGCGCACGGCAGAGCCTCCGCTGACGAGAACTCTGGATTTGTTTTTGTTAATTGCAGCATCGGCGGGACGGGCAGAGTGTGGCTCGGCCGCGCGTGGCGTCCGTTCTCTCGCGTCGTCTTTGCTAACACTGTCATGACGGATATTATTGCGCCGGAGGGTTGGAATGACTTTAATGACCCTGCAAGGGACCAGTACGTTTTCTTATTTTATGTATTTATTTGTTTTTTTTATCATTTTTTGAGGAAATACGTTTTCTTAATTTTAAACTTAGGGGAAAAAAGGTTAAATTGTGAAAAAAAAAAAAAAAACTTATTTTCATTTTTGGACGATAACTAAAAATTCAAGTGGTTAGTTAAGAAAAATAAAAATTATGATAAATAAATTTTAGGGAAACAAATATAAATTTTTAAAAACAAAAAATAAAATATAATTATCCAATGAGACTTAAAAATTTTATTTTGACATTTGAAGATTGTTTGAAAACAATTTTGGTCGATTTTCAACATTAAATTTAATGATGAAAATGTTTGCAAGACACGTAGTGCATTTATTAACTATATTATTGTCATCTTCATTTCAAAAACAATCCAAATTATCCAACTATGACAAGTATTTTCTTCTTATTATTATTATTAGTGATTGGAGTGAATTGTACAAATATGGATGTAGTTCTTAATACATCGAAGTATTATTTCAATACTTGGGTGCAATTACTCTCATCACAATGAATTAAAATATTTTAAAAAAGAAGTAAGAAGAATTTTCGATATGATAAATTTTGATGGAAGAATTTAGTGAGAGACAGAAAGATAGTTATTTTGCAATTAAATTAACATCAAAGACGAATATATTGATGAGACAATGCAATTAAAAAAGAAATTGAATGAAAATGCAGGACAATCTTCTATGGAGAGTACAACTGCAATGGAGCAGGAGCCAATATGAGCTCAAGGGCTCCCTTTGTTCAGAGGCTTAATGATACTCAAGCTTCCCCTTTCCTCAACTTATCCTTCATTGATGCTGACCAATGGCTCCAACCTTATGCATTCTTTTAACTCAACATCCCTATTCTCCTTATTTAATTCACTCCTTTTTTTCAATACTATATATTCTTTTCATTCTCACCCCATCTAATTATTAAATCTCAATTCTATTTTAAAACTTTGCTCTCTACATTCATCTATATCTACTTCCTAAAACTTATGTTTTA

mRNA sequence

CTATTTTACACCCAAAACTTTCATTCTTCATATTATTTATACACTTTTCTTCTTGTATTCCCTTCTCCATACTCCACACATAGGACCAATGAATAATATTATGAAGTATAATCATGTCCTCACCAATCTTGCTTTATCATCTCTTCTCCCTCTCCTTGCTTTCCTCTTCATGATTAAGCCCACCCCTCCCTTCCTTTCCAATCTTGTCATCTTTCGCGACTCCATCGGCTGTCATCACCATCACCATCATCACCACCACCACCATCATCGCCGTCATCATAAAAACGCAGCCGACGAAGAAGACCAAGCAGCCATAAGTTCCATTTGTGATACCTTCCCGATCACCCTCCTCCCACCAGAAACCCCCAACACTTCTGTATTTTGTGTTGATCGAAACGGTTGTTGCAACTTCACGACTGTTCAAGCGGCAGTCGACGCTGTGCCGATCTTGAGCTTCAAACGAAACATAATTTGGATCAACATTGGAATTTACTATGAGAAAGTGATAGTTCCAAAATCTAAAGCTAATGTGACATTTCAAGGACAAGGGTATACAACAACTGCCATTGTTTGGAATGACACTGCCAATTCTTCCCATGGCACCTTTTATAGTGGCTCTGTTCAAGTCTTCTCTTCCAATTTTATTGCCAAGAATATTAGCTTCATGAATGTGGCGCCAATTCCAGGCCCGGGCGACGTCGGAGCACAGGCGGTGGCGATTAGAATCGGAGGAGACCAGGCCGCGTTTTGGAGCTGCGGATTCTTCGGTGCTCAAGACACTCTCCACGACGACCGTGGCCGCCATTACTTCAAAGATTGCTACATCCAAGGCTCCATCGATTTCATCTTCGGCAATGGAAGATCCTTCTACGAGAGTTGCCAGTTGATTTCGATGGCGAATCCGGTTCCGCAAGGATCGAAGTTTGTGAACGGAGCGGTGACGGCGCACGGCAGAGCCTCCGCTGACGAGAACTCTGGATTTGTTTTTGTTAATTGCAGCATCGGCGGGACGGGCAGAGTGTGGCTCGGCCGCGCGTGGCGTCCGTTCTCTCGCGTCGTCTTTGCTAACACTGTCATGACGGATATTATTGCGCCGGAGGGTTGGAATGACTTTAATGACCCTGCAAGGGACCAGACAATCTTCTATGGAGAGTACAACTGCAATGGAGCAGGAGCCAATATGAGCTCAAGGGCTCCCTTTGTTCAGAGGCTTAATGATACTCAAGCTTCCCCTTTCCTCAACTTATCCTTCATTGATGCTGACCAATGGCTCCAACCTTATGCATTCTTTTAACTCAACATCCCTATTCTCCTTATTTAATTCACTCCTTTTTTTCAATACTATATATTCTTTTCATTCTCACCCCATCTAATTATTAAATCTCAATTCTATTTTAAAACTTTGCTCTCTACATTCATCTATATCTACTTCCTAAAACTTATGTTTTA

Coding sequence (CDS)

ATGAATAATATTATGAAGTATAATCATGTCCTCACCAATCTTGCTTTATCATCTCTTCTCCCTCTCCTTGCTTTCCTCTTCATGATTAAGCCCACCCCTCCCTTCCTTTCCAATCTTGTCATCTTTCGCGACTCCATCGGCTGTCATCACCATCACCATCATCACCACCACCACCATCATCGCCGTCATCATAAAAACGCAGCCGACGAAGAAGACCAAGCAGCCATAAGTTCCATTTGTGATACCTTCCCGATCACCCTCCTCCCACCAGAAACCCCCAACACTTCTGTATTTTGTGTTGATCGAAACGGTTGTTGCAACTTCACGACTGTTCAAGCGGCAGTCGACGCTGTGCCGATCTTGAGCTTCAAACGAAACATAATTTGGATCAACATTGGAATTTACTATGAGAAAGTGATAGTTCCAAAATCTAAAGCTAATGTGACATTTCAAGGACAAGGGTATACAACAACTGCCATTGTTTGGAATGACACTGCCAATTCTTCCCATGGCACCTTTTATAGTGGCTCTGTTCAAGTCTTCTCTTCCAATTTTATTGCCAAGAATATTAGCTTCATGAATGTGGCGCCAATTCCAGGCCCGGGCGACGTCGGAGCACAGGCGGTGGCGATTAGAATCGGAGGAGACCAGGCCGCGTTTTGGAGCTGCGGATTCTTCGGTGCTCAAGACACTCTCCACGACGACCGTGGCCGCCATTACTTCAAAGATTGCTACATCCAAGGCTCCATCGATTTCATCTTCGGCAATGGAAGATCCTTCTACGAGAGTTGCCAGTTGATTTCGATGGCGAATCCGGTTCCGCAAGGATCGAAGTTTGTGAACGGAGCGGTGACGGCGCACGGCAGAGCCTCCGCTGACGAGAACTCTGGATTTGTTTTTGTTAATTGCAGCATCGGCGGGACGGGCAGAGTGTGGCTCGGCCGCGCGTGGCGTCCGTTCTCTCGCGTCGTCTTTGCTAACACTGTCATGACGGATATTATTGCGCCGGAGGGTTGGAATGACTTTAATGACCCTGCAAGGGACCAGACAATCTTCTATGGAGAGTACAACTGCAATGGAGCAGGAGCCAATATGAGCTCAAGGGCTCCCTTTGTTCAGAGGCTTAATGATACTCAAGCTTCCCCTTTCCTCAACTTATCCTTCATTGATGCTGACCAATGGCTCCAACCTTATGCATTCTTTTAA

Protein sequence

MNNIMKYNHVLTNLALSSLLPLLAFLFMIKPTPPFLSNLVIFRDSIGCHHHHHHHHHHHHRRHHKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVDRNGCCNFTTVQAAVDAVPILSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFVFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCNGAGANMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQPYAFF
Homology
BLAST of Bhi01M002368 vs. TAIR 10
Match: AT1G05310.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 530.8 bits (1366), Expect = 9.6e-151
Identity = 250/355 (70.42%), Postives = 291/355 (81.97%), Query Frame = 0

Query: 42  FRDSIGCHHHHHHHHHHHHRRHHKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVD 101
           F+ S+  HHHHHHHHHHHH RH  +     D     SICD FP  + P +T  TS  CVD
Sbjct: 40  FKKSLCDHHHHHHHHHHHHHRHKPS-----DTKRKVSICDDFPKNIPPLDTDTTSYLCVD 99

Query: 102 RNGCCNFTTVQAAVDAVPILSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIV 161
           +NGCCNFTTVQ+AVDAV   S +RN+IWIN G+YYEKV++PK+K N+T QGQG+  TAI 
Sbjct: 100 KNGCCNFTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIA 159

Query: 162 WNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFW 221
           WNDTA S++GTFY  +VQVF S F+AKNISFMNVAPIP PGDVGAQAVAIRI GD++AF 
Sbjct: 160 WNDTAYSANGTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAFV 219

Query: 222 SCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVN 281
            CGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGN +S Y+ C++ISMAN +  GSK VN
Sbjct: 220 GCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAVN 279

Query: 282 GAVTAHGRASADENSGFVFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWND 341
           GAVTA+GR+S DENSGF FVNC+IGGTG VWLGRAWRP+SRVVF +T MTD+IAPEGWN+
Sbjct: 280 GAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNN 339

Query: 342 FNDPARDQTIFYGEYNCNGAGANMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQ 397
           FNDP+RD TIFYGEYNC+G GA+MS RAP+VQ+LN+TQ +  +N SFID DQWLQ
Sbjct: 340 FNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQVALLINTSFIDGDQWLQ 389

BLAST of Bhi01M002368 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 355.1 bits (910), Expect = 7.2e-98
Identity = 179/348 (51.44%), Postives = 236/348 (67.82%), Query Frame = 0

Query: 49  HHHHHHHHHHHHRRHHKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVDRNGCCNF 108
           HHHHHHHHHHHH  HH               C+ +  + L  +   + V  VD +GC NF
Sbjct: 52  HHHHHHHHHHHHHYHHHEPI---------KCCEKW-TSRLRHQYKTSLVLTVDLHGCGNF 111

Query: 109 TTVQAAVDAVPILSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTANS 168
           + VQ+A+D VP LS  + +I +N G Y EKV V ++K N+  QG+GY  T+I WNDTA S
Sbjct: 112 SNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQNTSIEWNDTAKS 171

Query: 169 SHGTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGA 228
           +  T  S S  VF++NF A NISF N AP P PG+  AQAVA+RI GDQAAF+ CGF+GA
Sbjct: 172 AGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRIEGDQAAFYGCGFYGA 231

Query: 229 QDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHG 288
           QDTL DD+GRH+FK+C+IQGSIDFIFGNGRS Y+ C + S+A     G   V G++TA G
Sbjct: 232 QDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGNTSG---VTGSITAQG 291

Query: 289 RASADENSGFVFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARD 348
           R S DE SGF FVNC I G+G + LGRAW  ++ VVF+NT M+ II PEGWN++ D  ++
Sbjct: 292 RQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITPEGWNNWGDSTKE 351

Query: 349 QTIFYGEYNCNGAGANMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQ 397
           +T+ +GE+ C G GA+   R  F ++L D++AS F+++SFID D+WL+
Sbjct: 352 KTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWLR 386

BLAST of Bhi01M002368 vs. TAIR 10
Match: AT2G36700.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 326.6 bits (836), Expect = 2.7e-89
Identity = 156/301 (51.83%), Postives = 207/301 (68.77%), Query Frame = 0

Query: 95  TSVFCVDRNGCCNFTTVQAAVDAVPILSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQG 154
           T V  V  NGC  F  VQ A+DA    S  + +I I+ GIY E+ IV ++K N+  QG G
Sbjct: 39  TMVLKVSLNGCGRFKRVQDAIDASIGSSQSKTLILIDFGIYRERFIVHENKNNLVVQGMG 98

Query: 155 YTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIG 214
           Y+ T+I WN+T  SS+GTF S SV VF   F A NISF N AP P PG V AQAVA+++ 
Sbjct: 99  YSRTSIEWNNTTASSNGTFSSFSVAVFGEKFTAYNISFKNTAPAPNPGAVDAQAVALKVV 158

Query: 215 GDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVP 274
           GD+AAF+ CGF+G QDTL D  GRH+FK C+I+GSIDFIFGNGRS YE C L S+A    
Sbjct: 159 GDKAAFYGCGFYGNQDTLLDQEGRHFFKGCFIEGSIDFIFGNGRSLYEDCTLHSIA---- 218

Query: 275 QGSKFVNGAVTAHGRASADENSGFVFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDII 334
              +   G +TA+G+ +  + +GFVFVNC I G+ RVWLGRAWRP++RV+F+ T M+ ++
Sbjct: 219 --KENTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRPYARVIFSKTYMSRVV 278

Query: 335 APEGWNDFNDPARDQTIFYGEYNCNGAGANMSSRAPFVQRLNDTQASPFLNLSFIDADQW 394
           + +GWND  DP   +T++YGE+ C G GAN S R  + + L+D +A+PF N+SFID ++W
Sbjct: 279 SLDGWNDMGDPKTQRTVYYGEHRCYGPGANHSKRVTYAKLLSDVEAAPFTNISFIDGEEW 333

Query: 395 L 396
           L
Sbjct: 339 L 333

BLAST of Bhi01M002368 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 309.7 bits (792), Expect = 3.5e-84
Identity = 146/296 (49.32%), Postives = 201/296 (67.91%), Query Frame = 0

Query: 107 NFTTVQAAVDAVPILSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTA 166
           +FT +Q A+D++P+++F R +I ++ G+Y EKV +P  KA +T +G+G   T + W DTA
Sbjct: 95  DFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTA 154

Query: 167 NSSH------GTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAF 226
            +        GT+ S S  V S  F+AKNI+F N  P+P PG VG QAVA+R+  D AAF
Sbjct: 155 QTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAF 214

Query: 227 WSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFV 286
           + C   GAQDTL+D  GRHY+KDCYI+GS+DFIFGN  S YE C + ++A+ +       
Sbjct: 215 FGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKL------- 274

Query: 287 NGAVTAHGRASADENSGFVFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWN 346
            GAVTA GR+S  E++GF FV C + GTG ++LGRAW PFSRVVFA T M +II P GW 
Sbjct: 275 -GAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWY 334

Query: 347 DFNDPARDQTIFYGEYNCNGAGANMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQ 397
           ++ DP+R+ T+FYG+Y C GAGAN   R  + + L D +A PFL+L+FID  +W++
Sbjct: 335 NWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

BLAST of Bhi01M002368 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 267.7 bits (683), Expect = 1.5e-71
Identity = 142/308 (46.10%), Postives = 196/308 (63.64%), Query Frame = 0

Query: 97  VFCVDRNGCCNFTTVQAAVDAVPILSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYT 156
           V  V  NG   F +VQ AVD++P  + K   I I  G Y EKV+VP +K  +TF+G G  
Sbjct: 60  VITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRD 119

Query: 157 TTAIVWNDTAN--SSHG----TFYSGSVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVA 216
            TAI W+D A+   ++G    T+ + SV V+++ F A+NISF N AP P PG  G QAVA
Sbjct: 120 VTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVA 179

Query: 217 IRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYESCQLISMA 276
            RI GD+A F  CGF+GAQDTL DD GRHYFK+CYI+GSIDFIFGNGRS Y+ C+L S+A
Sbjct: 180 FRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA 239

Query: 277 NPVPQGSKFVNGAVTAHGRASADENSGFVFVNCSIGGTGRVWLGRAWRPFSRVVFANTVM 336
                 S+F  G++ AHGR   +E +GF FV C + GTG +++GRA   +SR+V+A T  
Sbjct: 240 ------SRF--GSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYF 299

Query: 337 TDIIAPEGWNDFNDPA-RDQTIFYGEYNCNGAGANMSSRAPFVQRLNDTQASPFLNLSFI 396
             ++A  GW+D++  + + +T F+G YNC G GA  +    + + L+   A PF+  SF+
Sbjct: 300 DALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFV 359

Query: 397 DADQWLQP 398
           +   W+ P
Sbjct: 360 NGRHWIAP 359

BLAST of Bhi01M002368 vs. ExPASy Swiss-Prot
Match: O23038 (Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2)

HSP 1 Score: 530.8 bits (1366), Expect = 1.3e-149
Identity = 250/355 (70.42%), Postives = 291/355 (81.97%), Query Frame = 0

Query: 42  FRDSIGCHHHHHHHHHHHHRRHHKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVD 101
           F+ S+  HHHHHHHHHHHH RH  +     D     SICD FP  + P +T  TS  CVD
Sbjct: 40  FKKSLCDHHHHHHHHHHHHHRHKPS-----DTKRKVSICDDFPKNIPPLDTDTTSYLCVD 99

Query: 102 RNGCCNFTTVQAAVDAVPILSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIV 161
           +NGCCNFTTVQ+AVDAV   S +RN+IWIN G+YYEKV++PK+K N+T QGQG+  TAI 
Sbjct: 100 KNGCCNFTTVQSAVDAVGNFSQRRNVIWINSGMYYEKVVIPKTKPNITLQGQGFDITAIA 159

Query: 162 WNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFW 221
           WNDTA S++GTFY  +VQVF S F+AKNISFMNVAPIP PGDVGAQAVAIRI GD++AF 
Sbjct: 160 WNDTAYSANGTFYCATVQVFGSQFVAKNISFMNVAPIPKPGDVGAQAVAIRIAGDESAFV 219

Query: 222 SCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVN 281
            CGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGN +S Y+ C++ISMAN +  GSK VN
Sbjct: 220 GCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNAKSLYQDCRIISMANQLSPGSKAVN 279

Query: 282 GAVTAHGRASADENSGFVFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWND 341
           GAVTA+GR+S DENSGF FVNC+IGGTG VWLGRAWRP+SRVVF +T MTD+IAPEGWN+
Sbjct: 280 GAVTANGRSSKDENSGFSFVNCTIGGTGHVWLGRAWRPYSRVVFVSTTMTDVIAPEGWNN 339

Query: 342 FNDPARDQTIFYGEYNCNGAGANMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQ 397
           FNDP+RD TIFYGEYNC+G GA+MS RAP+VQ+LN+TQ +  +N SFID DQWLQ
Sbjct: 340 FNDPSRDATIFYGEYNCSGPGADMSKRAPYVQKLNETQVALLINTSFIDGDQWLQ 389

BLAST of Bhi01M002368 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 1.0e-96
Identity = 179/348 (51.44%), Postives = 236/348 (67.82%), Query Frame = 0

Query: 49  HHHHHHHHHHHHRRHHKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVDRNGCCNF 108
           HHHHHHHHHHHH  HH               C+ +  + L  +   + V  VD +GC NF
Sbjct: 52  HHHHHHHHHHHHHYHHHEPI---------KCCEKW-TSRLRHQYKTSLVLTVDLHGCGNF 111

Query: 109 TTVQAAVDAVPILSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTANS 168
           + VQ+A+D VP LS  + +I +N G Y EKV V ++K N+  QG+GY  T+I WNDTA S
Sbjct: 112 SNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQGRGYQNTSIEWNDTAKS 171

Query: 169 SHGTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGA 228
           +  T  S S  VF++NF A NISF N AP P PG+  AQAVA+RI GDQAAF+ CGF+GA
Sbjct: 172 AGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALRIEGDQAAFYGCGFYGA 231

Query: 229 QDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHG 288
           QDTL DD+GRH+FK+C+IQGSIDFIFGNGRS Y+ C + S+A     G   V G++TA G
Sbjct: 232 QDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKGNTSG---VTGSITAQG 291

Query: 289 RASADENSGFVFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARD 348
           R S DE SGF FVNC I G+G + LGRAW  ++ VVF+NT M+ II PEGWN++ D  ++
Sbjct: 292 RQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIITPEGWNNWGDSTKE 351

Query: 349 QTIFYGEYNCNGAGANMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQ 397
           +T+ +GE+ C G GA+   R  F ++L D++AS F+++SFID D+WL+
Sbjct: 352 KTVTFGEHKCYGPGADYKERVLFGKQLTDSEASSFIDVSFIDGDEWLR 386

BLAST of Bhi01M002368 vs. ExPASy Swiss-Prot
Match: Q9ZQA4 (Putative pectinesterase 14 OS=Arabidopsis thaliana OX=3702 GN=PME14 PE=2 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 3.9e-88
Identity = 156/301 (51.83%), Postives = 207/301 (68.77%), Query Frame = 0

Query: 95  TSVFCVDRNGCCNFTTVQAAVDAVPILSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQG 154
           T V  V  NGC  F  VQ A+DA    S  + +I I+ GIY E+ IV ++K N+  QG G
Sbjct: 39  TMVLKVSLNGCGRFKRVQDAIDASIGSSQSKTLILIDFGIYRERFIVHENKNNLVVQGMG 98

Query: 155 YTTTAIVWNDTANSSHGTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIG 214
           Y+ T+I WN+T  SS+GTF S SV VF   F A NISF N AP P PG V AQAVA+++ 
Sbjct: 99  YSRTSIEWNNTTASSNGTFSSFSVAVFGEKFTAYNISFKNTAPAPNPGAVDAQAVALKVV 158

Query: 215 GDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVP 274
           GD+AAF+ CGF+G QDTL D  GRH+FK C+I+GSIDFIFGNGRS YE C L S+A    
Sbjct: 159 GDKAAFYGCGFYGNQDTLLDQEGRHFFKGCFIEGSIDFIFGNGRSLYEDCTLHSIA---- 218

Query: 275 QGSKFVNGAVTAHGRASADENSGFVFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDII 334
              +   G +TA+G+ +  + +GFVFVNC I G+ RVWLGRAWRP++RV+F+ T M+ ++
Sbjct: 219 --KENTIGCITANGKDTLKDRTGFVFVNCKITGSARVWLGRAWRPYARVIFSKTYMSRVV 278

Query: 335 APEGWNDFNDPARDQTIFYGEYNCNGAGANMSSRAPFVQRLNDTQASPFLNLSFIDADQW 394
           + +GWND  DP   +T++YGE+ C G GAN S R  + + L+D +A+PF N+SFID ++W
Sbjct: 279 SLDGWNDMGDPKTQRTVYYGEHRCYGPGANHSKRVTYAKLLSDVEAAPFTNISFIDGEEW 333

Query: 395 L 396
           L
Sbjct: 339 L 333

BLAST of Bhi01M002368 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 4.9e-83
Identity = 146/296 (49.32%), Postives = 201/296 (67.91%), Query Frame = 0

Query: 107 NFTTVQAAVDAVPILSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTA 166
           +FT +Q A+D++P+++F R +I ++ G+Y EKV +P  KA +T +G+G   T + W DTA
Sbjct: 95  DFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVEWGDTA 154

Query: 167 NSSH------GTFYSGSVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAF 226
            +        GT+ S S  V S  F+AKNI+F N  P+P PG VG QAVA+R+  D AAF
Sbjct: 155 QTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSADNAAF 214

Query: 227 WSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFV 286
           + C   GAQDTL+D  GRHY+KDCYI+GS+DFIFGN  S YE C + ++A+ +       
Sbjct: 215 FGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKL------- 274

Query: 287 NGAVTAHGRASADENSGFVFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWN 346
            GAVTA GR+S  E++GF FV C + GTG ++LGRAW PFSRVVFA T M +II P GW 
Sbjct: 275 -GAVTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWY 334

Query: 347 DFNDPARDQTIFYGEYNCNGAGANMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQ 397
           ++ DP+R+ T+FYG+Y C GAGAN   R  + + L D +A PFL+L+FID  +W++
Sbjct: 335 NWGDPSREMTVFYGQYKCTGAGANYGGRVAWARELTDEEAKPFLSLTFIDGSEWIK 382

BLAST of Bhi01M002368 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 2.1e-70
Identity = 142/308 (46.10%), Postives = 196/308 (63.64%), Query Frame = 0

Query: 97  VFCVDRNGCCNFTTVQAAVDAVPILSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYT 156
           V  V  NG   F +VQ AVD++P  + K   I I  G Y EKV+VP +K  +TF+G G  
Sbjct: 60  VITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRD 119

Query: 157 TTAIVWNDTAN--SSHG----TFYSGSVQVFSSNFIAKNISFMNVAPIPGPGDVGAQAVA 216
            TAI W+D A+   ++G    T+ + SV V+++ F A+NISF N AP P PG  G QAVA
Sbjct: 120 VTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVA 179

Query: 217 IRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKDCYIQGSIDFIFGNGRSFYESCQLISMA 276
            RI GD+A F  CGF+GAQDTL DD GRHYFK+CYI+GSIDFIFGNGRS Y+ C+L S+A
Sbjct: 180 FRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA 239

Query: 277 NPVPQGSKFVNGAVTAHGRASADENSGFVFVNCSIGGTGRVWLGRAWRPFSRVVFANTVM 336
                 S+F  G++ AHGR   +E +GF FV C + GTG +++GRA   +SR+V+A T  
Sbjct: 240 ------SRF--GSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYF 299

Query: 337 TDIIAPEGWNDFNDPA-RDQTIFYGEYNCNGAGANMSSRAPFVQRLNDTQASPFLNLSFI 396
             ++A  GW+D++  + + +T F+G YNC G GA  +    + + L+   A PF+  SF+
Sbjct: 300 DALVAHGGWDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARALDYESAHPFIAKSFV 359

Query: 397 DADQWLQP 398
           +   W+ P
Sbjct: 360 NGRHWIAP 359

BLAST of Bhi01M002368 vs. NCBI nr
Match: XP_038903629.1 (probable pectinesterase 8 [Benincasa hispida])

HSP 1 Score: 825.9 bits (2132), Expect = 1.5e-235
Identity = 401/401 (100.00%), Postives = 401/401 (100.00%), Query Frame = 0

Query: 1   MNNIMKYNHVLTNLALSSLLPLLAFLFMIKPTPPFLSNLVIFRDSIGCHHHHHHHHHHHH 60
           MNNIMKYNHVLTNLALSSLLPLLAFLFMIKPTPPFLSNLVIFRDSIGCHHHHHHHHHHHH
Sbjct: 1   MNNIMKYNHVLTNLALSSLLPLLAFLFMIKPTPPFLSNLVIFRDSIGCHHHHHHHHHHHH 60

Query: 61  RRHHKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVDRNGCCNFTTVQAAVDAVPI 120
           RRHHKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVDRNGCCNFTTVQAAVDAVPI
Sbjct: 61  RRHHKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVDRNGCCNFTTVQAAVDAVPI 120

Query: 121 LSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQV 180
           LSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQV
Sbjct: 121 LSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQV 180

Query: 181 FSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHY 240
           FSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHY
Sbjct: 181 FSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHY 240

Query: 241 FKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFVF 300
           FKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFVF
Sbjct: 241 FKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFVF 300

Query: 301 VNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCNG 360
           VNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCNG
Sbjct: 301 VNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCNG 360

Query: 361 AGANMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQPYAFF 402
           AGANMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQPYAFF
Sbjct: 361 AGANMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQPYAFF 401

BLAST of Bhi01M002368 vs. NCBI nr
Match: XP_008451885.1 (PREDICTED: probable pectinesterase 8 [Cucumis melo])

HSP 1 Score: 719.2 bits (1855), Expect = 2.0e-203
Identity = 352/402 (87.56%), Postives = 367/402 (91.29%), Query Frame = 0

Query: 1   MNNIM-KYNHVLTNLALSSLLPLLAFLFMIKPTPPFLSNLVIFRDSIGCHHHHHHHHHHH 60
           MNNIM KYN+ L NL LSSLLPLLAFLFMI PTP +LSNLV+F D + C H HHHHHH  
Sbjct: 1   MNNIMTKYNNFLINLTLSSLLPLLAFLFMINPTPSYLSNLVVFGDPVDCPHPHHHHHHED 60

Query: 61  HRRHHKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVDRNGCCNFTTVQAAVDAVP 120
                    DE+DQ+ ISSICD FP  L+PPET NTSVFCVD+NGCCNFTTVQAAVDAVP
Sbjct: 61  --------PDEDDQSVISSICDNFPTNLVPPETTNTSVFCVDQNGCCNFTTVQAAVDAVP 120

Query: 121 ILSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ 180
            LS KRNIIWIN GIYYEKV+VPKSK NVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ
Sbjct: 121 NLSIKRNIIWINAGIYYEKVMVPKSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ 180

Query: 181 VFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRH 240
           VFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIR+GGDQAAFWSCGFFGAQDTLHDDRGRH
Sbjct: 181 VFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRVGGDQAAFWSCGFFGAQDTLHDDRGRH 240

Query: 241 YFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFV 300
           YFKDCYIQGSIDFIFGNGRSFYESCQL+SMANPVPQGSKFVNGAVTAHGRASA+ENSGF 
Sbjct: 241 YFKDCYIQGSIDFIFGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASAEENSGFS 300

Query: 301 FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCN 360
           FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDP RDQTIFYGEYNC+
Sbjct: 301 FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPTRDQTIFYGEYNCS 360

Query: 361 GAGANMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQPYAFF 402
           GAGANMSSRAP+VQRLNDTQ S FLNLSFIDADQWLQPY+FF
Sbjct: 361 GAGANMSSRAPYVQRLNDTQVSSFLNLSFIDADQWLQPYSFF 394

BLAST of Bhi01M002368 vs. NCBI nr
Match: XP_004147773.1 (probable pectinesterase 8 [Cucumis sativus] >KGN53483.1 hypothetical protein Csa_014669 [Cucumis sativus])

HSP 1 Score: 715.3 bits (1845), Expect = 2.9e-202
Identity = 347/401 (86.53%), Postives = 364/401 (90.77%), Query Frame = 0

Query: 1   MNNIMKYNHVLTNLALSSLLPLLAFLFMIKPTPPFLSNLVIFRDSIGCHHHHHHHHHHHH 60
           MN+IM YNH L NL LSSLLPLLAFLFMI PTP FLSNLV+F D + C HHHH HHHH +
Sbjct: 1   MNHIMNYNHFLINLTLSSLLPLLAFLFMINPTPSFLSNLVVFGDPVDCPHHHHPHHHHEN 60

Query: 61  RRHHKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVDRNGCCNFTTVQAAVDAVPI 120
                   DE+DQ+ ISSICD FP  L+P ET NTS+FCVD+N CCNFTT+QAAVDAVP 
Sbjct: 61  -------PDEDDQSVISSICDNFPTALVPLETTNTSIFCVDQNSCCNFTTIQAAVDAVPN 120

Query: 121 LSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQV 180
           LS KRNIIWIN GIYYEKV+VPK+KANVTFQGQGYTTTAIVWNDTANSSHGTFYS SVQV
Sbjct: 121 LSIKRNIIWINAGIYYEKVMVPKTKANVTFQGQGYTTTAIVWNDTANSSHGTFYSASVQV 180

Query: 181 FSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHY 240
           FSSNFIAKN+SFMNVAPIP PGDVGAQ VAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHY
Sbjct: 181 FSSNFIAKNLSFMNVAPIPAPGDVGAQGVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHY 240

Query: 241 FKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFVF 300
           FKDCYIQGSIDFIFGNGRSFYESCQL+SMANPVPQGSKFVNGAVTAHGRASADENSGF F
Sbjct: 241 FKDCYIQGSIDFIFGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASADENSGFSF 300

Query: 301 VNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCNG 360
           VNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNC+G
Sbjct: 301 VNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSG 360

Query: 361 AGANMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQPYAFF 402
           AGANMSSRAP+VQRLNDTQ S FLNLSFIDADQWLQPY+ F
Sbjct: 361 AGANMSSRAPYVQRLNDTQVSSFLNLSFIDADQWLQPYSLF 394

BLAST of Bhi01M002368 vs. NCBI nr
Match: XP_022136420.1 (probable pectinesterase 8 [Momordica charantia])

HSP 1 Score: 684.5 bits (1765), Expect = 5.5e-193
Identity = 338/400 (84.50%), Postives = 358/400 (89.50%), Query Frame = 0

Query: 1   MNNIMKYNHVLTNLALSSLLPLLAFLFMIKPTPPFLSNL--VIFRDSIGCHHHHHHHHHH 60
           MNN+   N+++T +ALSSL+PLLAFL +  P P FLS+L     RD + C  HHHHHHHH
Sbjct: 1   MNNL---NNLMT-IALSSLIPLLAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHH 60

Query: 61  HHRRHHKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVDRNGCCNFTTVQAAVDAV 120
              R H++ ADEEDQAA SSICD FP +LLP ET  TSVFCVDRNGCCNFTTVQAAVDAV
Sbjct: 61  ---RRHESPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAV 120

Query: 121 PILSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTANSSHGTFYSGSV 180
           P LS KRN+IWIN GIYYEKV+VP+SK NVTF+GQGYTTTAIVWNDTANSSHGTFYS SV
Sbjct: 121 PNLSLKRNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASV 180

Query: 181 QVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGR 240
           QVFSSNFIAKNISFMNVAPIPGPGDVGAQAVA+RIGGD AAFWSCGFFGAQDTLHDDRGR
Sbjct: 181 QVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGR 240

Query: 241 HYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGF 300
           HYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGF
Sbjct: 241 HYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGF 300

Query: 301 VFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNC 360
            FVNCS+ GTGRVWLGRAWRPFSRVVF NTVMTDIIAPEGWNDFNDPARDQTIFYGEYNC
Sbjct: 301 AFVNCSVAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNC 360

Query: 361 NGAGANMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQPY 399
           +GAGAN  +RAPFVQRLNDTQ SPFLNLSFIDADQWL PY
Sbjct: 361 SGAGANTGARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 393

BLAST of Bhi01M002368 vs. NCBI nr
Match: KAG7015100.1 (putative pectinesterase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 681.8 bits (1758), Expect = 3.6e-192
Identity = 333/395 (84.30%), Postives = 358/395 (90.63%), Query Frame = 0

Query: 5   MKYNHVLTNLALSSLLPL-LAFLFMIKPTPPFLSNLVIFRDSIGCHHHHHHHHHHHHRRH 64
           M  N+VL N+ALSSL+PL LA L ++ PTPPFLS   +  DS+ CHHH           H
Sbjct: 1   MTRNNVLINIALSSLVPLFLALLLVVIPTPPFLS---VLDDSLSCHHH-----------H 60

Query: 65  HKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVDRNGCCNFTTVQAAVDAVPILSF 124
           HK++ADEED+A+I SICDTFP  LLPPET NTSV CVD+NGCCNFTTVQAAVDAV  +S 
Sbjct: 61  HKSSADEEDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVLNMSS 120

Query: 125 KRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSS 184
           KR++IWIN GIY+EKV+VP+SK NVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSS
Sbjct: 121 KRSLIWINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSS 180

Query: 185 NFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKD 244
           NFIAKNISFMNVAPIPGPG +GAQAVAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFKD
Sbjct: 181 NFIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKD 240

Query: 245 CYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFVFVNC 304
           CYIQGSIDFIFGNGRS+YESCQ+ISMANPVPQGSKFVNGAVTAHGRASADENSGF FVNC
Sbjct: 241 CYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNC 300

Query: 305 SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCNGAGA 364
           SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIA EGWNDFNDPARDQTIFYGEYNC+GAGA
Sbjct: 301 SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGA 360

Query: 365 NMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQPY 399
           NMS+RAP+VQRLNDTQASPFLNLSFIDADQWLQPY
Sbjct: 361 NMSARAPYVQRLNDTQASPFLNLSFIDADQWLQPY 381

BLAST of Bhi01M002368 vs. ExPASy TrEMBL
Match: A0A1S3BRY7 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493043 PE=4 SV=1)

HSP 1 Score: 719.2 bits (1855), Expect = 9.8e-204
Identity = 352/402 (87.56%), Postives = 367/402 (91.29%), Query Frame = 0

Query: 1   MNNIM-KYNHVLTNLALSSLLPLLAFLFMIKPTPPFLSNLVIFRDSIGCHHHHHHHHHHH 60
           MNNIM KYN+ L NL LSSLLPLLAFLFMI PTP +LSNLV+F D + C H HHHHHH  
Sbjct: 1   MNNIMTKYNNFLINLTLSSLLPLLAFLFMINPTPSYLSNLVVFGDPVDCPHPHHHHHHED 60

Query: 61  HRRHHKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVDRNGCCNFTTVQAAVDAVP 120
                    DE+DQ+ ISSICD FP  L+PPET NTSVFCVD+NGCCNFTTVQAAVDAVP
Sbjct: 61  --------PDEDDQSVISSICDNFPTNLVPPETTNTSVFCVDQNGCCNFTTVQAAVDAVP 120

Query: 121 ILSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ 180
            LS KRNIIWIN GIYYEKV+VPKSK NVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ
Sbjct: 121 NLSIKRNIIWINAGIYYEKVMVPKSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQ 180

Query: 181 VFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRH 240
           VFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIR+GGDQAAFWSCGFFGAQDTLHDDRGRH
Sbjct: 181 VFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRVGGDQAAFWSCGFFGAQDTLHDDRGRH 240

Query: 241 YFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFV 300
           YFKDCYIQGSIDFIFGNGRSFYESCQL+SMANPVPQGSKFVNGAVTAHGRASA+ENSGF 
Sbjct: 241 YFKDCYIQGSIDFIFGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASAEENSGFS 300

Query: 301 FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCN 360
           FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDP RDQTIFYGEYNC+
Sbjct: 301 FVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPTRDQTIFYGEYNCS 360

Query: 361 GAGANMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQPYAFF 402
           GAGANMSSRAP+VQRLNDTQ S FLNLSFIDADQWLQPY+FF
Sbjct: 361 GAGANMSSRAPYVQRLNDTQVSSFLNLSFIDADQWLQPYSFF 394

BLAST of Bhi01M002368 vs. ExPASy TrEMBL
Match: A0A0A0KVA9 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G056710 PE=4 SV=1)

HSP 1 Score: 715.3 bits (1845), Expect = 1.4e-202
Identity = 347/401 (86.53%), Postives = 364/401 (90.77%), Query Frame = 0

Query: 1   MNNIMKYNHVLTNLALSSLLPLLAFLFMIKPTPPFLSNLVIFRDSIGCHHHHHHHHHHHH 60
           MN+IM YNH L NL LSSLLPLLAFLFMI PTP FLSNLV+F D + C HHHH HHHH +
Sbjct: 1   MNHIMNYNHFLINLTLSSLLPLLAFLFMINPTPSFLSNLVVFGDPVDCPHHHHPHHHHEN 60

Query: 61  RRHHKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVDRNGCCNFTTVQAAVDAVPI 120
                   DE+DQ+ ISSICD FP  L+P ET NTS+FCVD+N CCNFTT+QAAVDAVP 
Sbjct: 61  -------PDEDDQSVISSICDNFPTALVPLETTNTSIFCVDQNSCCNFTTIQAAVDAVPN 120

Query: 121 LSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQV 180
           LS KRNIIWIN GIYYEKV+VPK+KANVTFQGQGYTTTAIVWNDTANSSHGTFYS SVQV
Sbjct: 121 LSIKRNIIWINAGIYYEKVMVPKTKANVTFQGQGYTTTAIVWNDTANSSHGTFYSASVQV 180

Query: 181 FSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHY 240
           FSSNFIAKN+SFMNVAPIP PGDVGAQ VAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHY
Sbjct: 181 FSSNFIAKNLSFMNVAPIPAPGDVGAQGVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHY 240

Query: 241 FKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFVF 300
           FKDCYIQGSIDFIFGNGRSFYESCQL+SMANPVPQGSKFVNGAVTAHGRASADENSGF F
Sbjct: 241 FKDCYIQGSIDFIFGNGRSFYESCQLVSMANPVPQGSKFVNGAVTAHGRASADENSGFSF 300

Query: 301 VNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCNG 360
           VNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNC+G
Sbjct: 301 VNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCSG 360

Query: 361 AGANMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQPYAFF 402
           AGANMSSRAP+VQRLNDTQ S FLNLSFIDADQWLQPY+ F
Sbjct: 361 AGANMSSRAPYVQRLNDTQVSSFLNLSFIDADQWLQPYSLF 394

BLAST of Bhi01M002368 vs. ExPASy TrEMBL
Match: A0A6J1C3U9 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008131 PE=4 SV=1)

HSP 1 Score: 684.5 bits (1765), Expect = 2.7e-193
Identity = 338/400 (84.50%), Postives = 358/400 (89.50%), Query Frame = 0

Query: 1   MNNIMKYNHVLTNLALSSLLPLLAFLFMIKPTPPFLSNL--VIFRDSIGCHHHHHHHHHH 60
           MNN+   N+++T +ALSSL+PLLAFL +  P P FLS+L     RD + C  HHHHHHHH
Sbjct: 1   MNNL---NNLMT-IALSSLIPLLAFLLINHPAPRFLSDLDFAFLRDHVTCRRHHHHHHHH 60

Query: 61  HHRRHHKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVDRNGCCNFTTVQAAVDAV 120
              R H++ ADEEDQAA SSICD FP +LLP ET  TSVFCVDRNGCCNFTTVQAAVDAV
Sbjct: 61  ---RRHESPADEEDQAASSSICDMFPASLLPTETNTTSVFCVDRNGCCNFTTVQAAVDAV 120

Query: 121 PILSFKRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTANSSHGTFYSGSV 180
           P LS KRN+IWIN GIYYEKV+VP+SK NVTF+GQGYTTTAIVWNDTANSSHGTFYS SV
Sbjct: 121 PNLSLKRNLIWINSGIYYEKVMVPRSKQNVTFEGQGYTTTAIVWNDTANSSHGTFYSASV 180

Query: 181 QVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGR 240
           QVFSSNFIAKNISFMNVAPIPGPGDVGAQAVA+RIGGD AAFWSCGFFGAQDTLHDDRGR
Sbjct: 181 QVFSSNFIAKNISFMNVAPIPGPGDVGAQAVAMRIGGDAAAFWSCGFFGAQDTLHDDRGR 240

Query: 241 HYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGF 300
           HYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGF
Sbjct: 241 HYFKDCYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGF 300

Query: 301 VFVNCSIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNC 360
            FVNCS+ GTGRVWLGRAWRPFSRVVF NTVMTDIIAPEGWNDFNDPARDQTIFYGEYNC
Sbjct: 301 AFVNCSVAGTGRVWLGRAWRPFSRVVFVNTVMTDIIAPEGWNDFNDPARDQTIFYGEYNC 360

Query: 361 NGAGANMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQPY 399
           +GAGAN  +RAPFVQRLNDTQ SPFLNLSFIDADQWL PY
Sbjct: 361 SGAGANTGARAPFVQRLNDTQVSPFLNLSFIDADQWLHPY 393

BLAST of Bhi01M002368 vs. ExPASy TrEMBL
Match: A0A6J1ETE2 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437565 PE=4 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 7.3e-191
Identity = 330/395 (83.54%), Postives = 356/395 (90.13%), Query Frame = 0

Query: 5   MKYNHVLTNLALSSLLPL-LAFLFMIKPTPPFLSNLVIFRDSIGCHHHHHHHHHHHHRRH 64
           M +N++L N+ALSSL+PL LA L ++ PTPPF S   +  DS+ CHHH           H
Sbjct: 1   MTHNNLLINIALSSLVPLFLALLLVVIPTPPFFS---VPDDSLSCHHH-----------H 60

Query: 65  HKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVDRNGCCNFTTVQAAVDAVPILSF 124
           HK +ADEED+A+I SICDTFP  LLPPET NTSV CVD+NGCCNFTTVQAAVDAV  +S 
Sbjct: 61  HKPSADEEDRASIGSICDTFPTALLPPETVNTSVLCVDQNGCCNFTTVQAAVDAVLNMSS 120

Query: 125 KRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSS 184
           KR++IWIN GIY+EKV+VP+SK NVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSS
Sbjct: 121 KRSLIWINTGIYFEKVMVPRSKPNVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSS 180

Query: 185 NFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKD 244
           NFIAKNISFMNVAPIPGPG +GAQAVAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFKD
Sbjct: 181 NFIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKD 240

Query: 245 CYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFVFVNC 304
           CYIQGSIDFIFGNGRS+YESCQ+ISMANPVPQGSKFVNGAVTAHGRASADENSGF FVNC
Sbjct: 241 CYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNC 300

Query: 305 SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCNGAGA 364
           SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIA EGWNDFNDPARDQTIFYGEYNC+GAGA
Sbjct: 301 SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGAGA 360

Query: 365 NMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQPY 399
           NMS+RAP+VQRLNDTQ SPFLNLSFIDADQWLQPY
Sbjct: 361 NMSARAPYVQRLNDTQVSPFLNLSFIDADQWLQPY 381

BLAST of Bhi01M002368 vs. ExPASy TrEMBL
Match: A0A6J1J3X1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483170 PE=4 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 1.4e-189
Identity = 330/395 (83.54%), Postives = 353/395 (89.37%), Query Frame = 0

Query: 5   MKYNHVLTNLALSSLLPL-LAFLFMIKPTPPFLSNLVIFRDSIGCHHHHHHHHHHHHRRH 64
           M  N+VL N+ALSSL+PL LA L ++ PTPPFLS   +  DS+ CHHHH          H
Sbjct: 1   MTRNNVLINIALSSLVPLFLALLLVVIPTPPFLS---VLDDSLNCHHHH----------H 60

Query: 65  HKNAADEEDQAAISSICDTFPITLLPPETPNTSVFCVDRNGCCNFTTVQAAVDAVPILSF 124
           HK +ADEEDQ +I SICDTFP  LLP ET +TSV CVD NGCCNFTTVQAAVDAV  +S 
Sbjct: 61  HKPSADEEDQTSIGSICDTFPTALLPLETVDTSVLCVDLNGCCNFTTVQAAVDAVLNMSS 120

Query: 125 KRNIIWINIGIYYEKVIVPKSKANVTFQGQGYTTTAIVWNDTANSSHGTFYSGSVQVFSS 184
           KR++IWIN GIY+EKV+VP+SK NVTFQGQGY TTAIVWNDTANSSHGTFYSGSVQVFSS
Sbjct: 121 KRSLIWINTGIYFEKVMVPRSKPNVTFQGQGYKTTAIVWNDTANSSHGTFYSGSVQVFSS 180

Query: 185 NFIAKNISFMNVAPIPGPGDVGAQAVAIRIGGDQAAFWSCGFFGAQDTLHDDRGRHYFKD 244
           NFIAKNISFMNVAPIPGPG +GAQAVAIRIGGDQAAFW+CGFFGAQDTLHDDRGRHYFKD
Sbjct: 181 NFIAKNISFMNVAPIPGPGVIGAQAVAIRIGGDQAAFWNCGFFGAQDTLHDDRGRHYFKD 240

Query: 245 CYIQGSIDFIFGNGRSFYESCQLISMANPVPQGSKFVNGAVTAHGRASADENSGFVFVNC 304
           CYIQGSIDFIFGNGRS+YESCQ+ISMANPVPQGSKFVNGAVTAHGRASADENSGF FVNC
Sbjct: 241 CYIQGSIDFIFGNGRSYYESCQMISMANPVPQGSKFVNGAVTAHGRASADENSGFAFVNC 300

Query: 305 SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIAPEGWNDFNDPARDQTIFYGEYNCNGAGA 364
           SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIA EGWNDFNDPARDQTIFYGEYNC+G GA
Sbjct: 301 SIGGTGRVWLGRAWRPFSRVVFANTVMTDIIASEGWNDFNDPARDQTIFYGEYNCSGTGA 360

Query: 365 NMSSRAPFVQRLNDTQASPFLNLSFIDADQWLQPY 399
           NMS+RAP+VQRLNDTQASPFLNLSFIDADQWLQPY
Sbjct: 361 NMSARAPYVQRLNDTQASPFLNLSFIDADQWLQPY 382

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
AT1G05310.19.6e-15170.42Pectin lyase-like superfamily protein [more]
AT2G36710.17.2e-9851.44Pectin lyase-like superfamily protein [more]
AT2G36700.12.7e-8951.83Pectin lyase-like superfamily protein [more]
AT5G19730.13.5e-8449.32Pectin lyase-like superfamily protein [more]
AT5G47500.11.5e-7146.10Pectin lyase-like superfamily protein [more]
Match NameE-valueIdentityDescription
O230381.3e-14970.42Probable pectinesterase 8 OS=Arabidopsis thaliana OX=3702 GN=PME8 PE=2 SV=2[more]
Q9ZQA31.0e-9651.44Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1[more]
Q9ZQA43.9e-8851.83Putative pectinesterase 14 OS=Arabidopsis thaliana OX=3702 GN=PME14 PE=2 SV=1[more]
Q8VYZ34.9e-8349.32Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1[more]
Q8LPF32.1e-7046.10Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038903629.11.5e-235100.00probable pectinesterase 8 [Benincasa hispida][more]
XP_008451885.12.0e-20387.56PREDICTED: probable pectinesterase 8 [Cucumis melo][more]
XP_004147773.12.9e-20286.53probable pectinesterase 8 [Cucumis sativus] >KGN53483.1 hypothetical protein Csa... [more]
XP_022136420.15.5e-19384.50probable pectinesterase 8 [Momordica charantia][more]
KAG7015100.13.6e-19284.30putative pectinesterase 8, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A1S3BRY79.8e-20487.56Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103493043 PE=4 SV=1[more]
A0A0A0KVA91.4e-20286.53Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_4G056710 PE=4 SV=1[more]
A0A6J1C3U92.7e-19384.50Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111008131 PE=4 SV=1[more]
A0A6J1ETE27.3e-19183.54Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111437565 PE=4 SV=1[more]
A0A6J1J3X11.4e-18983.54Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111483170 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Wax gourd (B227) v1
Date Performed: 2021-10-22
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 94..400
e-value: 2.8E-99
score: 334.5
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 100..391
e-value: 1.8E-69
score: 233.8
NoneNo IPR availablePANTHERPTHR31321:SF33PECTINESTERASE 8-RELATEDcoord: 41..398
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 41..398
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 246..255
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 97..398

Relationships

This mRNA is a part of the following gene feature(s):

Feature NameUnique NameType
Bhi01G002368Bhi01G002368gene


The following exon feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi01M002368.exon5Bhi01M002368.exon5exon
Bhi01M002368.exon4Bhi01M002368.exon4exon
Bhi01M002368.exon3Bhi01M002368.exon3exon
Bhi01M002368.exon2Bhi01M002368.exon2exon
Bhi01M002368.exon1Bhi01M002368.exon1exon


The following three_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi01M002368.utr3p1Bhi01M002368.utr3p1three_prime_UTR


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameType
cds.Bhi01M002368cds.Bhi01M002368_5CDS
cds.Bhi01M002368cds.Bhi01M002368_4CDS
cds.Bhi01M002368cds.Bhi01M002368_3CDS
cds.Bhi01M002368cds.Bhi01M002368_2CDS
cds.Bhi01M002368cds.Bhi01M002368CDS


The following five_prime_UTR feature(s) are a part of this mRNA:

Feature NameUnique NameType
Bhi01M002368.utr5p1Bhi01M002368.utr5p1five_prime_UTR


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameType
Bhi01M002368Bhi01M002368-proteinpolypeptide


GO Annotation
GO Assignments
This mRNA is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0045330 aspartyl esterase activity