Tan0022707 (gene) Snake gourd v1

Overview


Name	Tan0022707
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Alpha-amylase
Location	LG01: 113876906 .. 113879108 (+)
RNA-Seq Expression	Tan0022707
Synteny	Tan0022707

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTTCTTCATTTTGAGATTGGAGATTCAAATCTCTATACTACACATTTGTTATACTAAAAAAACGTTTGAATTATTGTCCAAAAAATACGTTGGAATTTCTTACAAGTATTATTATTTCTGAAAAAGAGGCCTTTCGTAAATATCACCCTGTTGTCTCAAAGCCAAAGAGAGCTTACAAAAGCAAAATCATTTCTTACAGGTAAATATTATTTAATTTCTTAAGTTGGAAATTTCTTCTTTCTTCTTATCCTTTTTGGTTGTCGAGCATTCCTTCATTCATCAATCTTCCTTCCTTTCTTCAACAACTCGTTCTCACCAAGTCACAAAATTGACTTGTGTTGTTAGACCACTCAAACGCGCAAACAAAGCTTAATAAAAGCAAAATTCGCACTGCCCATTTCCAAAACAATGGAGTTTCTTGCTTCTCTGTGTTTCCTCTACCTCTCTGTTTCTCTCTTCCCATCCCCATCTTCCTCCACCTTACTCTTTCAGGTAAACAAATCCACTTTACTCTTCAAGTCTTCCTTATTCTCAAGCATTTTCCATTCATGTAAACTCACTTTTAAAAAAACCTTGAATGAATTGATGAAAAACAGGGCTTCAACTGGGAATCAAGCAGCAAAGGAGGATGGTACAACTCACTGAAGAACATAATTCCTGACCTTGAAAATGCTAGAATCACCCATGTCTGGCTTCCTCCACCCTCCCAATCAGCTGCTTCACAAGGCAACCCTTCTTTCTCTGTTCAATCACCTCATCAATCATCAATAACATTTCACTAAATTTGTGTTGCTAAAAACCCTCTTACAGGATACTTACCCGGAAGGCTTTATGATCTGAATGCATCAAAATATGGAACTCAAGATGAACTCAAGTCCTTAATTTCTGCCCTCCATGAGAAGGGAATCAAAGCCATTGCTGACATAGTGATCAACCACAGAACAGCAGAGAAACAGGACCAGAGAGGAATTTGGTGCATTTTTGAAGGGGGAACTTCAGATAGCCGCCTTGATTGGGGCCCTTCCTTCATTTGCAGCGATGACACTGATTATTCCGATGGAACGGGCAATCGAGACAGCGGCGAACCCTATCCACCAGCACCTGATATCGACCATCTCAATCCACAAGTTCAGAATGAAATATCAGAATGGATGAGGTGGTTGAAAACAGAAATAGGGTTCGATGGATGGAGATTCGATTTCGTAAAGGGCTATGCACCAAGTATTACAAAGCTCTACATGGATCGAACCTCACCAGATTTTGCAGTCGGCGAGAAATGGGATTCGCTTTCGTATGGACAGGACGGGAAGCCCAATCAGAACCAAGACGCACATCGCCATGCTCTTGCAGATTGGGTTAGGGCTTCGGGCGATACTGTCACAGCGTTTGATTTCACAACCAAAGGGATTCTTCAAGCTGCCGTTCAGGGGGAGCTATGGAGATTAAAGGATTCTAACGGGAAGCCGCCGGGATTGATCGGAATGAAGCCGGAAAGTGCCGTGACTTTCATCGACAATCACGACACTGGTTCAACTCAAAGAATCTGGCCGTTCCCTTCCGATAAAGTGATGCAGGGCTACGCATATATTCTCACTCATCCTGGAACGCCCTCCATAGTAATTCATTCAACTATACGGTTAAGATTTTGGAGTTTCGAAACTTAATTACCAGTAATTCACGCTCGGTTTGTTTGTTTGTTTGTTTGTTGCAGTTCTACGATCACTTTTTTGATTGGGGATTGAAGGAGGAGATAAGCAAATTGAGTGAAATCAGGGCAAGGAATGGGATTGAGAAAACGAGCACGGTGAATATTTTGGCTTCTGACGGCGATCTGTACGTTGCCGGTGTTGATGAGAAGATTATAATGAAGATCGGGCCGAACACTAATCTTGGAAATCTGATTCCGTCGGATTACGAGCTTGCTACTTCTGGAATGGAGTATGCTGTTTGGGAGAAAAAGTGAATTTCATGAATACTACTTGAAAAGCCGCCATTGATGGTATAATTCGTCTTGGAGGTCCATTTTCAGGAAAGTTTCGAAACTCGAAAGAGAATCCGATAAATCATTTTATAGATTAAAACTAAAAGATGTAACTTTTTTTTTACGCATAAATAAAATCTGATAACTTTGAGCATAGCACAATTTAGTTCATTAAATATTGTTCAACTAAAAAGAATTTGTGGCTGAAGTAAACCTACCGAA

mRNA sequence

GTTCTTCATTTTGAGATTGGAGATTCAAATCTCTATACTACACATTTGTTATACTAAAAAAACGTTTGAATTATTGTCCAAAAAATACGTTGGAATTTCTTACAAGTATTATTATTTCTGAAAAAGAGGCCTTTCGTAAATATCACCCTGTTGTCTCAAAGCCAAAGAGAGCTTACAAAAGCAAAATCATTTCTTACAGGTAAATATTATTTAATTTCTTAAGTTGGAAATTTCTTCTTTCTTCTTATCCTTTTTGGTTGTCGAGCATTCCTTCATTCATCAATCTTCCTTCCTTTCTTCAACAACTCGTTCTCACCAAGTCACAAAATTGACTTGTGTTGTTAGACCACTCAAACGCGCAAACAAAGCTTAATAAAAGCAAAATTCGCACTGCCCATTTCCAAAACAATGGAGTTTCTTGCTTCTCTGTGTTTCCTCTACCTCTCTGTTTCTCTCTTCCCATCCCCATCTTCCTCCACCTTACTCTTTCAGGGCTTCAACTGGGAATCAAGCAGCAAAGGAGGATGGTACAACTCACTGAAGAACATAATTCCTGACCTTGAAAATGCTAGAATCACCCATGTCTGGCTTCCTCCACCCTCCCAATCAGCTGCTTCACAAGGATACTTACCCGGAAGGCTTTATGATCTGAATGCATCAAAATATGGAACTCAAGATGAACTCAAGTCCTTAATTTCTGCCCTCCATGAGAAGGGAATCAAAGCCATTGCTGACATAGTGATCAACCACAGAACAGCAGAGAAACAGGACCAGAGAGGAATTTGGTGCATTTTTGAAGGGGGAACTTCAGATAGCCGCCTTGATTGGGGCCCTTCCTTCATTTGCAGCGATGACACTGATTATTCCGATGGAACGGGCAATCGAGACAGCGGCGAACCCTATCCACCAGCACCTGATATCGACCATCTCAATCCACAAGTTCAGAATGAAATATCAGAATGGATGAGGTGGTTGAAAACAGAAATAGGGTTCGATGGATGGAGATTCGATTTCGTAAAGGGCTATGCACCAAGTATTACAAAGCTCTACATGGATCGAACCTCACCAGATTTTGCAGTCGGCGAGAAATGGGATTCGCTTTCGTATGGACAGGACGGGAAGCCCAATCAGAACCAAGACGCACATCGCCATGCTCTTGCAGATTGGGTTAGGGCTTCGGGCGATACTGTCACAGCGTTTGATTTCACAACCAAAGGGATTCTTCAAGCTGCCGTTCAGGGGGAGCTATGGAGATTAAAGGATTCTAACGGGAAGCCGCCGGGATTGATCGGAATGAAGCCGGAAAGTGCCGTGACTTTCATCGACAATCACGACACTGGTTCAACTCAAAGAATCTGGCCGTTCCCTTCCGATAAAGTGATGCAGGGCTACGCATATATTCTCACTCATCCTGGAACGCCCTCCATATTCTACGATCACTTTTTTGATTGGGGATTGAAGGAGGAGATAAGCAAATTGAGTGAAATCAGGGCAAGGAATGGGATTGAGAAAACGAGCACGGTGAATATTTTGGCTTCTGACGGCGATCTGTACGTTGCCGGTGTTGATGAGAAGATTATAATGAAGATCGGGCCGAACACTAATCTTGGAAATCTGATTCCGTCGGATTACGAGCTTGCTACTTCTGGAATGGAGTATGCTGTTTGGGAGAAAAAGTGAATTTCATGAATACTACTTGAAAAGCCGCCATTGATGGTATAATTCGTCTTGGAGGTCCATTTTCAGGAAAGTTTCGAAACTCGAAAGAGAATCCGATAAATCATTTTATAGATTAAAACTAAAAGATGTAACTTTTTTTTTACGCATAAATAAAATCTGATAACTTTGAGCATAGCACAATTTAGTTCATTAAATATTGTTCAACTAAAAAGAATTTGTGGCTGAAGTAAACCTACCGAA

Coding sequence (CDS)

ATGGAGTTTCTTGCTTCTCTGTGTTTCCTCTACCTCTCTGTTTCTCTCTTCCCATCCCCATCTTCCTCCACCTTACTCTTTCAGGGCTTCAACTGGGAATCAAGCAGCAAAGGAGGATGGTACAACTCACTGAAGAACATAATTCCTGACCTTGAAAATGCTAGAATCACCCATGTCTGGCTTCCTCCACCCTCCCAATCAGCTGCTTCACAAGGATACTTACCCGGAAGGCTTTATGATCTGAATGCATCAAAATATGGAACTCAAGATGAACTCAAGTCCTTAATTTCTGCCCTCCATGAGAAGGGAATCAAAGCCATTGCTGACATAGTGATCAACCACAGAACAGCAGAGAAACAGGACCAGAGAGGAATTTGGTGCATTTTTGAAGGGGGAACTTCAGATAGCCGCCTTGATTGGGGCCCTTCCTTCATTTGCAGCGATGACACTGATTATTCCGATGGAACGGGCAATCGAGACAGCGGCGAACCCTATCCACCAGCACCTGATATCGACCATCTCAATCCACAAGTTCAGAATGAAATATCAGAATGGATGAGGTGGTTGAAAACAGAAATAGGGTTCGATGGATGGAGATTCGATTTCGTAAAGGGCTATGCACCAAGTATTACAAAGCTCTACATGGATCGAACCTCACCAGATTTTGCAGTCGGCGAGAAATGGGATTCGCTTTCGTATGGACAGGACGGGAAGCCCAATCAGAACCAAGACGCACATCGCCATGCTCTTGCAGATTGGGTTAGGGCTTCGGGCGATACTGTCACAGCGTTTGATTTCACAACCAAAGGGATTCTTCAAGCTGCCGTTCAGGGGGAGCTATGGAGATTAAAGGATTCTAACGGGAAGCCGCCGGGATTGATCGGAATGAAGCCGGAAAGTGCCGTGACTTTCATCGACAATCACGACACTGGTTCAACTCAAAGAATCTGGCCGTTCCCTTCCGATAAAGTGATGCAGGGCTACGCATATATTCTCACTCATCCTGGAACGCCCTCCATATTCTACGATCACTTTTTTGATTGGGGATTGAAGGAGGAGATAAGCAAATTGAGTGAAATCAGGGCAAGGAATGGGATTGAGAAAACGAGCACGGTGAATATTTTGGCTTCTGACGGCGATCTGTACGTTGCCGGTGTTGATGAGAAGATTATAATGAAGATCGGGCCGAACACTAATCTTGGAAATCTGATTCCGTCGGATTACGAGCTTGCTACTTCTGGAATGGAGTATGCTGTTTGGGAGAAAAAGTGA

Protein sequence

MEFLASLCFLYLSVSLFPSPSSSTLLFQGFNWESSSKGGWYNSLKNIIPDLENARITHVWLPPPSQSAASQGYLPGRLYDLNASKYGTQDELKSLISALHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTSDSRLDWGPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQVQNEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRHALADWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGMKPESAVTFIDNHDTGSTQRIWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKLSEIRARNGIEKTSTVNILASDGDLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEYAVWEKK

Homology

[Close]

BLAST of Tan0022707 vs. ExPASy Swiss-Prot
Match: P17859 (Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 5.0e-203
Identity = 327/421 (77.67%), Postives = 368/421 (87.41%), Query Frame = 0


Query: 1   MEFLASLCFLYLSVSLFPSPSSSTLLFQGFNWESSSKGGWYNSLKNIIPDLENARITHVW 60
           M+  + L    L +SL P  SS  LLFQGFNWESS KGGWYNSLKN IPDL NA ITHVW
Sbjct: 1   MDSFSRLSIFCLFISLLPLFSSPALLFQGFNWESSKKGGWYNSLKNSIPDLANAGITHVW 60

Query: 61  LPPPSQSAASQGYLPGRLYDLNASKYGTQDELKSLISALHEKGIKAIADIVINHRTAEKQ 120
           LPPPSQS + +GYLPGRLYDL+ASKYG+++ELKSLI+A HEKGIK +ADIVINHRTAE++
Sbjct: 61  LPPPSQSVSPEGYLPGRLYDLDASKYGSKNELKSLIAAFHEKGIKCLADIVINHRTAERK 120

Query: 121 DQRGIWCIFEGGTSDSRLDWGPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQVQN 180
           D RGI+CIFEGGT DSR DWGPSFIC DDT YSDGTGN DSGE Y  APDIDHLNPQVQ 
Sbjct: 121 DGRGIYCIFEGGTPDSRQDWGPSFICRDDTAYSDGTGNNDSGEGYDAAPDIDHLNPQVQR 180

Query: 181 EISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGKPN 240
           E+SEWM WLKTEIGFDGWRFDFVKGYAPSI+K+YM++T PDFAVGEKWDS+SYGQDGKPN
Sbjct: 181 ELSEWMNWLKTEIGFDGWRFDFVKGYAPSISKIYMEQTKPDFAVGEKWDSISYGQDGKPN 240

Query: 241 QNQDAHRHALADWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGMKPES 300
            NQD+HR AL +WV ++G  +TAFDFTTKGILQAAVQGELWRL D NGKPPG+IG+KPE+
Sbjct: 241 YNQDSHRGALVNWVESAGGAITAFDFTTKGILQAAVQGELWRLIDPNGKPPGMIGVKPEN 300

Query: 301 AVTFIDNHDTGSTQRIWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKLSEI 360
           AVTFIDNHDTGSTQR+WPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKE+I+KLS I
Sbjct: 301 AVTFIDNHDTGSTQRLWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEQIAKLSSI 360

Query: 361 RARNGIEKTSTVNILASDGDLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEYAVW 420
           R RNGI + STV I+AS+GDLYVA +D KI++KIGP  +LGNLIPS+  +ATSG +YAVW
Sbjct: 361 RLRNGINEKSTVKIMASEGDLYVAKIDNKIMVKIGPKMDLGNLIPSNLHVATSGQDYAVW 420

Query: 421 E 422
           E
Sbjct: 421 E 421

[Close]

BLAST of Tan0022707 vs. ExPASy Swiss-Prot
Match: P27933 (Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.3 PE=2 SV=2)

HSP 1 Score: 579.3 bits (1492), Expect = 3.5e-164
Identity = 263/425 (61.88%), Postives = 321/425 (75.53%), Query Frame = 0


Query: 1   MEFLASLCFLYLSV--SLFPSPSSSTLLFQGFNWES-SSKGGWYNSLKNIIPDLENARIT 60
           M+  +SLC L L V  SL  +   + +LFQGFNWES   +GGWYN LK  + D+  A +T
Sbjct: 1   MKNTSSLCLLLLVVLCSLTCNSGQAQVLFQGFNWESWKQQGGWYNMLKGQVDDIAKAGVT 60

Query: 61  HVWLPPPSQSAASQGYLPGRLYDLNASKYGTQDELKSLISALHEKGIKAIADIVINHRTA 120
           HVWLPPPS S A QGY+PGRLYDL+ASKYGT  ELKSLI+A H KG++ +AD+VINHR A
Sbjct: 61  HVWLPPPSHSVAPQGYMPGRLYDLDASKYGTAAELKSLIAAFHGKGVQCVADVVINHRCA 120

Query: 121 EKQDQRGIWCIFEGGTSDSRLDWGPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQ 180
           EK+D RG++C+FEGGT D RLDWGP  ICSDDT YSDGTG+RD+GE +  APDIDHLNP+
Sbjct: 121 EKKDARGVYCVFEGGTPDDRLDWGPGMICSDDTQYSDGTGHRDTGEGFGAAPDIDHLNPR 180

Query: 181 VQNEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDG 240
           VQ E+++W+ WLK+++GFDGWR DF KGY+  I K+Y++   P F V E W+SLSY  DG
Sbjct: 181 VQRELTDWLNWLKSDVGFDGWRLDFAKGYSTDIAKMYVESCKPGFVVAEIWNSLSYNGDG 240

Query: 241 KPNQNQDAHRHALADWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGMK 300
           KP  NQD  R  L +WV A G     FDFTTKG+LQA VQGELWRL+D NGK  G+IG  
Sbjct: 241 KPAANQDQGRQELVNWVNAVGGPAMTFDFTTKGLLQAGVQGELWRLRDGNGKAAGMIGWL 300

Query: 301 PESAVTFIDNHDTGSTQRIWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKL 360
           PE AVTF+DNHDTGSTQ++WPFPSDKVMQGYAYILTHPG P IFYDH FDW LK+EI+ L
Sbjct: 301 PEKAVTFVDNHDTGSTQKLWPFPSDKVMQGYAYILTHPGVPCIFYDHMFDWNLKQEITAL 360

Query: 361 SEIRARNGIEKTSTVNILASDGDLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEY 420
           + IR RNGI   S + I+ +D D YVA VDEK+++KIG   ++GN +PSD+     G +Y
Sbjct: 361 AAIRERNGINAGSKLRIVVADADAYVAVVDEKVMVKIGTRYDVGNAVPSDFHQTVHGKDY 420

Query: 421 AVWEK 423
           +VWEK
Sbjct: 421 SVWEK 425

[Close]

BLAST of Tan0022707 vs. ExPASy Swiss-Prot
Match: Q8VZ56 (Alpha-amylase 1 OS=Arabidopsis thaliana OX=3702 GN=AMY1 PE=1 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 5.0e-163
Identity = 270/404 (66.83%), Postives = 326/404 (80.69%), Query Frame = 0


Query: 22  SSTLLFQGFNWESSSK-GGWYNSLKNIIPDLENARITHVWLPPPSQSAASQGYLPGRLYD 81
           SSTLLFQ FNWES  K GG+YNSL N I D+ NA ITH+WLPPPSQS A +GYLPG+LYD
Sbjct: 24  SSTLLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYD 83

Query: 82  LNASKYGTQDELKSLISALHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTSDSRLDW 141
           LN+SKYG++ ELKSLI AL++KGIKA+ADIVINHRTAE++D +  +C FEGGTSD RLDW
Sbjct: 84  LNSSKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDW 143

Query: 142 GPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQVQNEISEWMRWLKTEIGFDGWRF 201
            PSF+C +D  +  GTGN D+G  +  APDIDHLNP+VQ E+SEWM WLKTEIGF GWRF
Sbjct: 144 DPSFVCRNDPKF-PGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRF 203

Query: 202 DFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRHALADWV-RASGD 261
           D+V+GYA SITKLY+  TSPDFAVGEKWD + YG DGK + +Q+ HR  L  W+  A G 
Sbjct: 204 DYVRGYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGG 263

Query: 262 TVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGMKPESAVTFIDNHDTGSTQRIWPF 321
            +TAFDFTTKGILQ+AV+GELWRLKDS GKPPG+IG+ P +AVTFIDNHD   T R W F
Sbjct: 264 VLTAFDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHD---TFRTWVF 323

Query: 322 PSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKLSEIRARNGIEKTSTVNILASDG 381
           PSDKV+ GY YILTHPGTP IFY+H+ +WGLKE ISKL  IR +NGI  TS+V I A++ 
Sbjct: 324 PSDKVLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSVTIKAAEA 383

Query: 382 DLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEYAVWEKK 424
           DLY+A +D+K+IMKIGP  ++G L+PS++ LA SG+++AVWEKK
Sbjct: 384 DLYLAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWEKK 423

[Close]

BLAST of Tan0022707 vs. ExPASy Swiss-Prot
Match: P04063 (Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3)

HSP 1 Score: 574.7 bits (1480), Expect = 8.6e-163
Identity = 265/420 (63.10%), Postives = 318/420 (75.71%), Query Frame = 0


Query: 6   SLCFLYLSVSLFPSPSSSTLLFQGFNWES-SSKGGWYNSLKNIIPDLENARITHVWLPPP 65
           SL    + + L  S +S  +LFQGFNWES    GGWYN L   + D+  A ITHVWLPP 
Sbjct: 7   SLSLFLVLLGLSASLASGQVLFQGFNWESWKHNGGWYNFLMGKVDDIAAAGITHVWLPPA 66

Query: 66  SQSAASQGYLPGRLYDLNASKYGTQDELKSLISALHEKGIKAIADIVINHRTAEKQDQRG 125
           SQS A QGY+PGRLYDL+ASKYG + +LKSLI ALH KG+KAIADIVINHRTAE +D RG
Sbjct: 67  SQSVAEQGYMPGRLYDLDASKYGNKAQLKSLIGALHGKGVKAIADIVINHRTAEHKDGRG 126

Query: 126 IWCIFEGGTSDSRLDWGPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQVQNEISE 185
           I+CIFEGGT D+RLDWGP  IC DD  Y+DGTGN D+G  +  APDIDHLN +VQ E+ E
Sbjct: 127 IYCIFEGGTPDARLDWGPHMICRDDRPYADGTGNPDTGADFGAAPDIDHLNLRVQKELVE 186

Query: 186 WMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGKPNQNQD 245
           W+ WLK +IGFDGWRFDF KGY+  + K+Y+DR+ P FAV E W SL+YG DGKPN NQD
Sbjct: 187 WLNWLKADIGFDGWRFDFAKGYSADVAKIYIDRSEPSFAVAEIWTSLAYGGDGKPNLNQD 246

Query: 246 AHRHALADWVRASG--DTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGMKPESAV 305
            HR  L +WV   G     T FDFTTKGIL  AV+GELWRL+ ++GK PG+IG  P  AV
Sbjct: 247 QHRQELVNWVDKVGGKGPATTFDFTTKGILNVAVEGELWRLRGTDGKAPGMIGWWPAKAV 306

Query: 306 TFIDNHDTGSTQRIWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKLSEIRA 365
           TF+DNHDTGSTQ +WPFPSD+VMQGYAYILTHPGTP IFYDHFFDWGLKEEI +L  +R 
Sbjct: 307 TFVDNHDTGSTQHMWPFPSDRVMQGYAYILTHPGTPCIFYDHFFDWGLKEEIDRLVSVRT 366

Query: 366 RNGIEKTSTVNILASDGDLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEYAVWEK 423
           R+GI   S + I+ +D DLY+A +D K+I+K+GP  ++GNLIP  +++A  G +YAVWEK
Sbjct: 367 RHGIHNESKLQIIEADADLYLAEIDGKVIVKLGPRYDVGNLIPGGFKVAAHGNDYAVWEK 426

[Close]

BLAST of Tan0022707 vs. ExPASy Swiss-Prot
Match: P27934 (Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 1.9e-162
Identity = 254/417 (60.91%), Postives = 319/417 (76.50%), Query Frame = 0


Query: 8   CFLYLSVSLFPSPSSSTLLFQGFNWESSSK-GGWYNSLKNIIPDLENARITHVWLPPPSQ 67
           C ++  + L  S + + +LFQGFNWES  K GGWYN L   + ++ +   THVWLPPPS 
Sbjct: 10  CVVFAVLCLASSLAQAQVLFQGFNWESWRKQGGWYNFLHEKVEEIASTGATHVWLPPPSH 69

Query: 68  SAASQGYLPGRLYDLNASKYGTQDELKSLISALHEKGIKAIADIVINHRTAEKQDQRGIW 127
           S + QGY+PGRLYDL+ASKYGT+ ELKSLI A H+K ++ +ADIVINHR A+ +D RG++
Sbjct: 70  SVSPQGYMPGRLYDLDASKYGTEAELKSLIEAFHDKNVECLADIVINHRCADYKDSRGVY 129

Query: 128 CIFEGGTSDSRLDWGPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQVQNEISEWM 187
           C+FEGGT D RLDWGP  ICSDDT YS+G G+RD+G  +  APDIDHLNP+VQ E+++W+
Sbjct: 130 CVFEGGTPDGRLDWGPDMICSDDTQYSNGRGHRDTGAGFGAAPDIDHLNPRVQRELTDWL 189

Query: 188 RWLKTEIGFDGWRFDFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGKPNQNQDAH 247
            WL+T++GFDGWR DF KGY+  + ++Y+D T+P F VGE W SL Y  DGKP+ NQDA 
Sbjct: 190 NWLRTDLGFDGWRLDFAKGYSAPLARIYVDNTNPTFVVGEIWSSLIYNGDGKPSTNQDAD 249

Query: 248 RHALADWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGMKPESAVTFID 307
           R  L +WV   G   TAFDFTTKGILQAAVQGELWRL D NGK PGL+G  P+ AVTF+D
Sbjct: 250 RQELVNWVEGVGKPATAFDFTTKGILQAAVQGELWRLHDGNGKAPGLMGWMPDQAVTFVD 309

Query: 308 NHDTGSTQRIWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKLSEIRARNGI 367
           NHDTGSTQ +WPFPSDKVMQGYAYILTHPG P IFYDH FDW L+ EI+ L+EIR+RNGI
Sbjct: 310 NHDTGSTQSLWPFPSDKVMQGYAYILTHPGIPCIFYDHVFDWNLQHEIATLAEIRSRNGI 369

Query: 368 EKTSTVNILASDGDLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEYAVWEKK 424
              ST++IL ++GD+YVA +D K+I K+GP  + G +IPSD+ +   G +Y VWEK+
Sbjct: 370 HAESTLDILKAEGDIYVAMIDGKVITKLGPRYDAGGIIPSDFHVVAHGNDYCVWEKE 426

[Close]

BLAST of Tan0022707 vs. NCBI nr
Match: XP_038896126.1 (alpha-amylase-like [Benincasa hispida])

HSP 1 Score: 801.2 bits (2068), Expect = 4.3e-228
Identity = 378/424 (89.15%), Postives = 400/424 (94.34%), Query Frame = 0


Query: 1   MEFLASLCFLYLSVSLFPSP-SSSTLLFQGFNWESSSKGGWYNSLKNIIPDLENARITHV 60
           M FLASLCFLYL VSLFPSP SSSTLLFQGFNWESSSKGGWYNSL NIIPDL +A +THV
Sbjct: 1   MGFLASLCFLYLCVSLFPSPSSSSTLLFQGFNWESSSKGGWYNSLNNIIPDLLSAEVTHV 60

Query: 61  WLPPPSQSAASQGYLPGRLYDLNASKYGTQDELKSLISALHEKGIKAIADIVINHRTAEK 120
           WLPPPSQS +SQGYLPGRLYDLNASKYGTQ+ELKSLI+ LHEKGIKAIADIVINHRTAEK
Sbjct: 61  WLPPPSQSVSSQGYLPGRLYDLNASKYGTQNELKSLIATLHEKGIKAIADIVINHRTAEK 120

Query: 121 QDQRGIWCIFEGGTSDSRLDWGPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQVQ 180
           QDQRGIWCIFEGGT DSRLDWGPSFICSDDT+YSDG GN DSGEPYPPAPDIDHLNPQVQ
Sbjct: 121 QDQRGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYPPAPDIDHLNPQVQ 180

Query: 181 NEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGKP 240
            E+SEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMD+TSP+FAVGEKWD LSYGQDGKP
Sbjct: 181 KELSEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDQTSPEFAVGEKWDGLSYGQDGKP 240

Query: 241 NQNQDAHRHALADWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGMKPE 300
           NQNQD+HR ALA+WVRASGD VTAFDFTTKGILQAAV+GELWRLKDSNG PPG IG+KPE
Sbjct: 241 NQNQDSHRDALANWVRASGDIVTAFDFTTKGILQAAVEGELWRLKDSNGNPPGFIGIKPE 300

Query: 301 SAVTFIDNHDTGSTQRIWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKLSE 360
           SAVTFI+NHDTGSTQR+WPFPSDKV+QGYAYILTHPGTPSIFYDHF DWGLKEEI KLS 
Sbjct: 301 SAVTFIENHDTGSTQRMWPFPSDKVIQGYAYILTHPGTPSIFYDHFVDWGLKEEIMKLSG 360

Query: 361 IRARNGIEKTSTVNILASDGDLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEYAV 420
           IRARNGI KTSTVNI+AS+GDLYVAGVD KIIMKIG NT+LGNLIPSDY+LAT GM+YAV
Sbjct: 361 IRARNGIGKTSTVNIMASEGDLYVAGVDGKIIMKIGANTSLGNLIPSDYQLATYGMDYAV 420

Query: 421 WEKK 424
           WEK+
Sbjct: 421 WEKR 424

[Close]

BLAST of Tan0022707 vs. NCBI nr
Match: XP_022142476.1 (alpha-amylase [Momordica charantia])

HSP 1 Score: 800.0 bits (2065), Expect = 9.6e-228
Identity = 377/423 (89.13%), Postives = 399/423 (94.33%), Query Frame = 0


Query: 1   MEFLASLCFLYLSVSLFPSPSSSTLLFQGFNWESSSKGGWYNSLKNIIPDLENARITHVW 60
           M FLASLCFLYLSVSLFPSPSSS LLFQGFNWESSSKGGWYNSLKNIIPDL NARITHVW
Sbjct: 16  MGFLASLCFLYLSVSLFPSPSSSALLFQGFNWESSSKGGWYNSLKNIIPDLVNARITHVW 75

Query: 61  LPPPSQSAASQGYLPGRLYDLNASKYGTQDELKSLISALHEKGIKAIADIVINHRTAEKQ 120
           LPPPSQS + QGYLPGRLYDL+ASKYGTQ+ELKSLI+ALHEKGIKAIADIVINHRTAEKQ
Sbjct: 76  LPPPSQSVSPQGYLPGRLYDLDASKYGTQNELKSLIAALHEKGIKAIADIVINHRTAEKQ 135

Query: 121 DQRGIWCIFEGGTSDSRLDWGPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQVQN 180
           DQRGIWCIFEGGT D+RLDWGPSFICSDDTDYSDG GN DSGEPYPPAPDIDHL PQVQ 
Sbjct: 136 DQRGIWCIFEGGTPDNRLDWGPSFICSDDTDYSDGKGNPDSGEPYPPAPDIDHLKPQVQK 195

Query: 181 EISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGKPN 240
           EISEWMRWLKTEIGFDGWRFDFVKGYAPS+TKLYMD+TSPDFAVGEKWDSLS G DGK N
Sbjct: 196 EISEWMRWLKTEIGFDGWRFDFVKGYAPSVTKLYMDQTSPDFAVGEKWDSLSDGPDGKLN 255

Query: 241 QNQDAHRHALADWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGMKPES 300
           QNQDAHR +LA+WV+ASGD VTAFDFTTKGILQAAVQGELWRLKDSNGKPPG IG+KPES
Sbjct: 256 QNQDAHRDSLANWVKASGDAVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPES 315

Query: 301 AVTFIDNHDTGSTQRIWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKLSEI 360
           AVTF+DNHDTGSTQ++WPFPSDKV+QGYAYILTHPGTP+IFYDHF +WGLKEEI +LSEI
Sbjct: 316 AVTFVDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPAIFYDHFVEWGLKEEIIRLSEI 375

Query: 361 RARNGIEKTSTVNILASDGDLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEYAVW 420
           RARN I KTS VNILASD DLYVAGVD KIIMKIG NTNLGNLIPS+++LATSGMEYAVW
Sbjct: 376 RARNRIGKTSAVNILASDADLYVAGVDGKIIMKIGANTNLGNLIPSEFQLATSGMEYAVW 435

Query: 421 EKK 424
           EK+
Sbjct: 436 EKR 438

[Close]

BLAST of Tan0022707 vs. NCBI nr
Match: XP_022936258.1 (alpha-amylase, partial [Cucurbita moschata])

HSP 1 Score: 785.8 bits (2028), Expect = 1.9e-223
Identity = 371/425 (87.29%), Postives = 396/425 (93.18%), Query Frame = 0


Query: 1   MEFLASLCFL-YLSVSLFPSPS-SSTLLFQGFNWESSSKGGWYNSLKNIIPDLENARITH 60
           M FLASLCFL YLSV LFPSPS SSTLLFQGFNWESSSKGGWYNSL NIIPDL +A++TH
Sbjct: 25  MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 84

Query: 61  VWLPPPSQSAASQGYLPGRLYDLNASKYGTQDELKSLISALHEKGIKAIADIVINHRTAE 120
           VWLPPPSQSA+ QGYLPGRLYDLNASKYGTQ+ELKSLI+ALH  GIKA+ADIVINHRTA+
Sbjct: 85  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQNELKSLIAALHNNGIKAVADIVINHRTAD 144

Query: 121 KQDQRGIWCIFEGGTSDSRLDWGPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQV 180
           KQD+RGIWCIFEGGT DSRLDWGPSFICSDDT+YSDG GN DSGEPY  APDIDHLNPQV
Sbjct: 145 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQV 204

Query: 181 QNEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGK 240
           QNE+SEWMRWLKTEIGFDGWRFDFVKGYAP ITKLYMD+TSPDFAVGEKWDSLS G DGK
Sbjct: 205 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK 264

Query: 241 PNQNQDAHRHALADWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGMKP 300
           P+ NQD HR ALA+WVR SGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPG IG+KP
Sbjct: 265 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKP 324

Query: 301 ESAVTFIDNHDTGSTQRIWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKLS 360
           ESAVTFIDNHDTGSTQ++WPFPSDKV+QGYAYILTHPGTPSIFYDHF +WGLKEE+ KLS
Sbjct: 325 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 384

Query: 361 EIRARNGIEKTSTVNILASDGDLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEYA 420
            IRARNGI + STVNI+ASDGDLYVAG+D KIIMKIGPNTNLGNL+PSDYELA SGM+YA
Sbjct: 385 AIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYA 444

Query: 421 VWEKK 424
           VWEKK
Sbjct: 445 VWEKK 449

[Close]

BLAST of Tan0022707 vs. NCBI nr
Match: XP_022976296.1 (alpha-amylase [Cucurbita maxima])

HSP 1 Score: 785.4 bits (2027), Expect = 2.4e-223
Identity = 371/425 (87.29%), Postives = 395/425 (92.94%), Query Frame = 0


Query: 1   MEFLASLCFL-YLSVSLFPSPS-SSTLLFQGFNWESSSKGGWYNSLKNIIPDLENARITH 60
           M FLASLCFL YLSV +FPSPS SSTLLFQGFNWESSSKGGWYNSL NIIPDL +A++TH
Sbjct: 1   MGFLASLCFLCYLSVFIFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60

Query: 61  VWLPPPSQSAASQGYLPGRLYDLNASKYGTQDELKSLISALHEKGIKAIADIVINHRTAE 120
           VWLPPPSQSA+ QGYLPGRLYDLNASKYGTQDELKSLI+ALH  GIKA+ADIVINHRTA+
Sbjct: 61  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 120

Query: 121 KQDQRGIWCIFEGGTSDSRLDWGPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQV 180
           KQD+RGIWCIFEGGT DSRLDWGPSFICSDDT+YSDG GN DSGEPY  APDIDHLNPQV
Sbjct: 121 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQV 180

Query: 181 QNEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGK 240
           QNE+SEWMRWLKTEIGFDGWRFDFVKGYAP ITKLYMD+TSPDFAVGEKWDSLS G DGK
Sbjct: 181 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSPGPDGK 240

Query: 241 PNQNQDAHRHALADWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGMKP 300
           P+ NQD HR ALA+WVR SGDTVTAFDFTTKGILQAAVQGELWRLKD NGKPPG IG+KP
Sbjct: 241 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDPNGKPPGFIGIKP 300

Query: 301 ESAVTFIDNHDTGSTQRIWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKLS 360
           ESAVTFIDNHDTGSTQ++WPFPSDKV+QGYAYILTHPGTPSIFYDHF +WGLKEE+ KLS
Sbjct: 301 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 360

Query: 361 EIRARNGIEKTSTVNILASDGDLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEYA 420
            IR RNGI + STVNI+ASDGDLYVAG+D KIIMKIGPNTNLGNLIPSDYELATSGM+YA
Sbjct: 361 AIRTRNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLIPSDYELATSGMQYA 420

Query: 421 VWEKK 424
           VWEKK
Sbjct: 421 VWEKK 425

[Close]

BLAST of Tan0022707 vs. NCBI nr
Match: XP_023536126.1 (alpha-amylase, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 785.0 bits (2026), Expect = 3.2e-223
Identity = 371/425 (87.29%), Postives = 395/425 (92.94%), Query Frame = 0


Query: 1   MEFLASLCFL-YLSVSLFPSPS-SSTLLFQGFNWESSSKGGWYNSLKNIIPDLENARITH 60
           M FLASLCFL YLSV +FPSPS SSTLLFQGFNWESSSKGGWYNSL NIIPDL +A++TH
Sbjct: 43  MGFLASLCFLCYLSVFIFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 102

Query: 61  VWLPPPSQSAASQGYLPGRLYDLNASKYGTQDELKSLISALHEKGIKAIADIVINHRTAE 120
           VWLPPPSQSA+ QGYLPGRLYDLNASKYGTQDELKSLI+ALH  GIKA+ADIVINHRTA+
Sbjct: 103 VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 162

Query: 121 KQDQRGIWCIFEGGTSDSRLDWGPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQV 180
           KQD+RGIWCIFEGGT DSRLDWGPSFICSDDT+YSDG GN DSGEPY  APDIDHLNPQV
Sbjct: 163 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQV 222

Query: 181 QNEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGK 240
           QNE+SEWMRWLKTEIGFDGWRFDFVKGYAP ITKLYMD+TSPDFAVGEKWDSLS G DGK
Sbjct: 223 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK 282

Query: 241 PNQNQDAHRHALADWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGMKP 300
           P+ NQD HR ALA+WVR SGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPG IG+KP
Sbjct: 283 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKP 342

Query: 301 ESAVTFIDNHDTGSTQRIWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKLS 360
           ESAVTFIDNHDTGSTQ++WPFPSDKV+QGYAYILTHPGTPSIFYDHF +WGLKEE+ KLS
Sbjct: 343 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 402

Query: 361 EIRARNGIEKTSTVNILASDGDLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEYA 420
            IR RNGI + STVNI+ASDGDLYVAG+D KIIMKIGPNTNLGNLIPSDY LATSGM+YA
Sbjct: 403 AIRTRNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLIPSDYVLATSGMQYA 462

Query: 421 VWEKK 424
           VWEKK
Sbjct: 463 VWEKK 467

[Close]

BLAST of Tan0022707 vs. ExPASy TrEMBL
Match: A0A6J1CMA4 (Alpha-amylase OS=Momordica charantia OX=3673 GN=LOC111012587 PE=3 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 4.6e-228
Identity = 377/423 (89.13%), Postives = 399/423 (94.33%), Query Frame = 0


Query: 1   MEFLASLCFLYLSVSLFPSPSSSTLLFQGFNWESSSKGGWYNSLKNIIPDLENARITHVW 60
           M FLASLCFLYLSVSLFPSPSSS LLFQGFNWESSSKGGWYNSLKNIIPDL NARITHVW
Sbjct: 16  MGFLASLCFLYLSVSLFPSPSSSALLFQGFNWESSSKGGWYNSLKNIIPDLVNARITHVW 75

Query: 61  LPPPSQSAASQGYLPGRLYDLNASKYGTQDELKSLISALHEKGIKAIADIVINHRTAEKQ 120
           LPPPSQS + QGYLPGRLYDL+ASKYGTQ+ELKSLI+ALHEKGIKAIADIVINHRTAEKQ
Sbjct: 76  LPPPSQSVSPQGYLPGRLYDLDASKYGTQNELKSLIAALHEKGIKAIADIVINHRTAEKQ 135

Query: 121 DQRGIWCIFEGGTSDSRLDWGPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQVQN 180
           DQRGIWCIFEGGT D+RLDWGPSFICSDDTDYSDG GN DSGEPYPPAPDIDHL PQVQ 
Sbjct: 136 DQRGIWCIFEGGTPDNRLDWGPSFICSDDTDYSDGKGNPDSGEPYPPAPDIDHLKPQVQK 195

Query: 181 EISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGKPN 240
           EISEWMRWLKTEIGFDGWRFDFVKGYAPS+TKLYMD+TSPDFAVGEKWDSLS G DGK N
Sbjct: 196 EISEWMRWLKTEIGFDGWRFDFVKGYAPSVTKLYMDQTSPDFAVGEKWDSLSDGPDGKLN 255

Query: 241 QNQDAHRHALADWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGMKPES 300
           QNQDAHR +LA+WV+ASGD VTAFDFTTKGILQAAVQGELWRLKDSNGKPPG IG+KPES
Sbjct: 256 QNQDAHRDSLANWVKASGDAVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKPES 315

Query: 301 AVTFIDNHDTGSTQRIWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKLSEI 360
           AVTF+DNHDTGSTQ++WPFPSDKV+QGYAYILTHPGTP+IFYDHF +WGLKEEI +LSEI
Sbjct: 316 AVTFVDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPAIFYDHFVEWGLKEEIIRLSEI 375

Query: 361 RARNGIEKTSTVNILASDGDLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEYAVW 420
           RARN I KTS VNILASD DLYVAGVD KIIMKIG NTNLGNLIPS+++LATSGMEYAVW
Sbjct: 376 RARNRIGKTSAVNILASDADLYVAGVDGKIIMKIGANTNLGNLIPSEFQLATSGMEYAVW 435

Query: 421 EKK 424
           EK+
Sbjct: 436 EKR 438

[Close]

BLAST of Tan0022707 vs. ExPASy TrEMBL
Match: A0A6J1FCS4 (Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111442931 PE=3 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 9.0e-224
Identity = 371/425 (87.29%), Postives = 396/425 (93.18%), Query Frame = 0


Query: 1   MEFLASLCFL-YLSVSLFPSPS-SSTLLFQGFNWESSSKGGWYNSLKNIIPDLENARITH 60
           M FLASLCFL YLSV LFPSPS SSTLLFQGFNWESSSKGGWYNSL NIIPDL +A++TH
Sbjct: 25  MGFLASLCFLCYLSVFLFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 84

Query: 61  VWLPPPSQSAASQGYLPGRLYDLNASKYGTQDELKSLISALHEKGIKAIADIVINHRTAE 120
           VWLPPPSQSA+ QGYLPGRLYDLNASKYGTQ+ELKSLI+ALH  GIKA+ADIVINHRTA+
Sbjct: 85  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQNELKSLIAALHNNGIKAVADIVINHRTAD 144

Query: 121 KQDQRGIWCIFEGGTSDSRLDWGPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQV 180
           KQD+RGIWCIFEGGT DSRLDWGPSFICSDDT+YSDG GN DSGEPY  APDIDHLNPQV
Sbjct: 145 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQV 204

Query: 181 QNEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGK 240
           QNE+SEWMRWLKTEIGFDGWRFDFVKGYAP ITKLYMD+TSPDFAVGEKWDSLS G DGK
Sbjct: 205 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSSGPDGK 264

Query: 241 PNQNQDAHRHALADWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGMKP 300
           P+ NQD HR ALA+WVR SGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPG IG+KP
Sbjct: 265 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGFIGIKP 324

Query: 301 ESAVTFIDNHDTGSTQRIWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKLS 360
           ESAVTFIDNHDTGSTQ++WPFPSDKV+QGYAYILTHPGTPSIFYDHF +WGLKEE+ KLS
Sbjct: 325 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 384

Query: 361 EIRARNGIEKTSTVNILASDGDLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEYA 420
            IRARNGI + STVNI+ASDGDLYVAG+D KIIMKIGPNTNLGNL+PSDYELA SGM+YA
Sbjct: 385 AIRARNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLVPSDYELAASGMQYA 444

Query: 421 VWEKK 424
           VWEKK
Sbjct: 445 VWEKK 449

[Close]

BLAST of Tan0022707 vs. ExPASy TrEMBL
Match: A0A6J1IGI6 (Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111476734 PE=3 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 1.2e-223
Identity = 371/425 (87.29%), Postives = 395/425 (92.94%), Query Frame = 0


Query: 1   MEFLASLCFL-YLSVSLFPSPS-SSTLLFQGFNWESSSKGGWYNSLKNIIPDLENARITH 60
           M FLASLCFL YLSV +FPSPS SSTLLFQGFNWESSSKGGWYNSL NIIPDL +A++TH
Sbjct: 1   MGFLASLCFLCYLSVFIFPSPSFSSTLLFQGFNWESSSKGGWYNSLNNIIPDLVHAKVTH 60

Query: 61  VWLPPPSQSAASQGYLPGRLYDLNASKYGTQDELKSLISALHEKGIKAIADIVINHRTAE 120
           VWLPPPSQSA+ QGYLPGRLYDLNASKYGTQDELKSLI+ALH  GIKA+ADIVINHRTA+
Sbjct: 61  VWLPPPSQSASPQGYLPGRLYDLNASKYGTQDELKSLIAALHNNGIKAVADIVINHRTAD 120

Query: 121 KQDQRGIWCIFEGGTSDSRLDWGPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQV 180
           KQD+RGIWCIFEGGT DSRLDWGPSFICSDDT+YSDG GN DSGEPY  APDIDHLNPQV
Sbjct: 121 KQDERGIWCIFEGGTPDSRLDWGPSFICSDDTEYSDGKGNPDSGEPYSAAPDIDHLNPQV 180

Query: 181 QNEISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGK 240
           QNE+SEWMRWLKTEIGFDGWRFDFVKGYAP ITKLYMD+TSPDFAVGEKWDSLS G DGK
Sbjct: 181 QNELSEWMRWLKTEIGFDGWRFDFVKGYAPKITKLYMDQTSPDFAVGEKWDSLSPGPDGK 240

Query: 241 PNQNQDAHRHALADWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGMKP 300
           P+ NQD HR ALA+WVR SGDTVTAFDFTTKGILQAAVQGELWRLKD NGKPPG IG+KP
Sbjct: 241 PDPNQDGHREALANWVRDSGDTVTAFDFTTKGILQAAVQGELWRLKDPNGKPPGFIGIKP 300

Query: 301 ESAVTFIDNHDTGSTQRIWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKLS 360
           ESAVTFIDNHDTGSTQ++WPFPSDKV+QGYAYILTHPGTPSIFYDHF +WGLKEE+ KLS
Sbjct: 301 ESAVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHFVEWGLKEELIKLS 360

Query: 361 EIRARNGIEKTSTVNILASDGDLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEYA 420
            IR RNGI + STVNI+ASDGDLYVAG+D KIIMKIGPNTNLGNLIPSDYELATSGM+YA
Sbjct: 361 AIRTRNGIGERSTVNIMASDGDLYVAGIDGKIIMKIGPNTNLGNLIPSDYELATSGMQYA 420

Query: 421 VWEKK 424
           VWEKK
Sbjct: 421 VWEKK 425

[Close]

BLAST of Tan0022707 vs. ExPASy TrEMBL
Match: A0A2I4EXA7 (Alpha-amylase OS=Juglans regia OX=51240 GN=LOC108993530 PE=3 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 6.5e-206
Identity = 337/423 (79.67%), Postives = 374/423 (88.42%), Query Frame = 0


Query: 1   MEFLASLCFLYLSVSLFPSPSSSTLLFQGFNWESSSKGGWYNSLKNIIPDLENARITHVW 60
           M     LCFL L +SL  S ++ +LLFQGFNWESS KGGWYNSLKN +PDL NA ITHVW
Sbjct: 1   MNLKTFLCFLCLFISLCHSFAAPSLLFQGFNWESSKKGGWYNSLKNFVPDLANAEITHVW 60

Query: 61  LPPPSQSAASQGYLPGRLYDLNASKYGTQDELKSLISALHEKGIKAIADIVINHRTAEKQ 120
           LPPPSQSAA +GYLPGRLYDL+ SKYG++DELK+LI+ALHEKGIK +ADIVINHRTAEKQ
Sbjct: 61  LPPPSQSAAPEGYLPGRLYDLDVSKYGSKDELKALIAALHEKGIKVVADIVINHRTAEKQ 120

Query: 121 DQRGIWCIFEGGTSDSRLDWGPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQVQN 180
           D RGIW IFEGGT D+RLDWGPSFIC DD  YSDGTGN DSGE Y PAPDIDH NPQVQ 
Sbjct: 121 DDRGIWSIFEGGTPDARLDWGPSFICKDDIAYSDGTGNPDSGEGYDPAPDIDHRNPQVQK 180

Query: 181 EISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGKPN 240
           E+SEWM WLKTEIG+DGWRFDFVKGYAPSI K YM++T PDFAVGE W+SL+YG DGKPN
Sbjct: 181 ELSEWMNWLKTEIGYDGWRFDFVKGYAPSIMKNYMEQTKPDFAVGENWNSLAYGDDGKPN 240

Query: 241 QNQDAHRHALADWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGMKPES 300
            NQDAHR ALA+WV ++G  VTAFDFTTKGILQAAV+GELWRLKDSNGKPPGLIG+KPES
Sbjct: 241 PNQDAHRGALAEWVGSAGGAVTAFDFTTKGILQAAVEGELWRLKDSNGKPPGLIGIKPES 300

Query: 301 AVTFIDNHDTGSTQRIWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKLSEI 360
           AVTFIDNHDTGSTQ IWPFPSDKVMQGYAYILTHPGTP+IFYDHFFDWGLKEEISKL+ I
Sbjct: 301 AVTFIDNHDTGSTQNIWPFPSDKVMQGYAYILTHPGTPTIFYDHFFDWGLKEEISKLTAI 360

Query: 361 RARNGIEKTSTVNILASDGDLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEYAVW 420
           R+RNGI   S V+ILASDGDLYVAG+D+KIIMKIGP  +LGNLIPS+++++TSG +YAVW
Sbjct: 361 RSRNGINTRSIVSILASDGDLYVAGIDDKIIMKIGPKMDLGNLIPSNFQVSTSGKDYAVW 420

Query: 421 EKK 424
           EKK
Sbjct: 421 EKK 423

[Close]

BLAST of Tan0022707 vs. ExPASy TrEMBL
Match: A0A2N9FNN7 (Alpha-amylase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS20239 PE=3 SV=1)

HSP 1 Score: 722.2 bits (1863), Expect = 1.2e-204
Identity = 333/423 (78.72%), Postives = 373/423 (88.18%), Query Frame = 0


Query: 1   MEFLASLCFLYLSVSLFPSPSSSTLLFQGFNWESSSKGGWYNSLKNIIPDLENARITHVW 60
           M  L SLCFL L +SL PS +   +LFQGFNWESS+KGGWYN LKN IPDL NA ITHVW
Sbjct: 1   MNILTSLCFLCLCISLSPSFAVPAILFQGFNWESSNKGGWYNLLKNSIPDLANAGITHVW 60

Query: 61  LPPPSQSAASQGYLPGRLYDLNASKYGTQDELKSLISALHEKGIKAIADIVINHRTAEKQ 120
           LPPPSQS A QGY+PGRLYDL+ASKYG++DELKSLI+ALHEKGIKA+ADIVINHRTAEKQ
Sbjct: 61  LPPPSQSVAPQGYMPGRLYDLDASKYGSKDELKSLIAALHEKGIKALADIVINHRTAEKQ 120

Query: 121 DQRGIWCIFEGGTSDSRLDWGPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQVQN 180
           D RGIWCIFEGGT D RLDWGPSFIC DDT YSDGTGN DSG+ +  APDIDH+NPQVQ 
Sbjct: 121 DGRGIWCIFEGGTPDGRLDWGPSFICKDDTAYSDGTGNPDSGKDFGGAPDIDHVNPQVQK 180

Query: 181 EISEWMRWLKTEIGFDGWRFDFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGKPN 240
           E+SEWM WLKTEIGFDGWRFDFV GYA S TK+YM++T PDFAVGE W+SLSYG  GKPN
Sbjct: 181 ELSEWMNWLKTEIGFDGWRFDFVLGYASSFTKVYMEQTKPDFAVGENWNSLSYGDGGKPN 240

Query: 241 QNQDAHRHALADWVRASGDTVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGMKPES 300
            NQDAHR+AL DW++A+G  VTAFDFTTKGILQAAV+GELWRLKDSNGKPPGLIG+KPE+
Sbjct: 241 PNQDAHRNALVDWIQAAGGAVTAFDFTTKGILQAAVEGELWRLKDSNGKPPGLIGIKPEN 300

Query: 301 AVTFIDNHDTGSTQRIWPFPSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKLSEI 360
           AVTFIDNHDTGSTQ++WPFPSDKV+QGYAYILTHPGTPSIFYDH FDWGLK+EISKL+ I
Sbjct: 301 AVTFIDNHDTGSTQKLWPFPSDKVIQGYAYILTHPGTPSIFYDHLFDWGLKDEISKLTAI 360

Query: 361 RARNGIEKTSTVNILASDGDLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEYAVW 420
           R+RNGI     VNILASD DLYVAG+D+KIIMKIGP  +LGNLIPS+++++ SG +YAVW
Sbjct: 361 RSRNGINTQGIVNILASDADLYVAGIDDKIIMKIGPKMDLGNLIPSNFQVSASGKDYAVW 420

Query: 421 EKK 424
           EKK
Sbjct: 421 EKK 423

[Close]

BLAST of Tan0022707 vs. TAIR 10
Match: AT4G25000.1 (alpha-amylase-like )

HSP 1 Score: 575.5 bits (1482), Expect = 3.6e-164
Identity = 270/404 (66.83%), Postives = 326/404 (80.69%), Query Frame = 0


Query: 22  SSTLLFQGFNWESSSK-GGWYNSLKNIIPDLENARITHVWLPPPSQSAASQGYLPGRLYD 81
           SSTLLFQ FNWES  K GG+YNSL N I D+ NA ITH+WLPPPSQS A +GYLPG+LYD
Sbjct: 24  SSTLLFQSFNWESWKKEGGFYNSLHNSIDDIANAGITHLWLPPPSQSVAPEGYLPGKLYD 83

Query: 82  LNASKYGTQDELKSLISALHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTSDSRLDW 141
           LN+SKYG++ ELKSLI AL++KGIKA+ADIVINHRTAE++D +  +C FEGGTSD RLDW
Sbjct: 84  LNSSKYGSEAELKSLIKALNQKGIKALADIVINHRTAERKDDKCGYCYFEGGTSDDRLDW 143

Query: 142 GPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQVQNEISEWMRWLKTEIGFDGWRF 201
            PSF+C +D  +  GTGN D+G  +  APDIDHLNP+VQ E+SEWM WLKTEIGF GWRF
Sbjct: 144 DPSFVCRNDPKF-PGTGNLDTGGDFDGAPDIDHLNPRVQKELSEWMNWLKTEIGFHGWRF 203

Query: 202 DFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRHALADWV-RASGD 261
           D+V+GYA SITKLY+  TSPDFAVGEKWD + YG DGK + +Q+ HR  L  W+  A G 
Sbjct: 204 DYVRGYASSITKLYVQNTSPDFAVGEKWDDMKYGGDGKLDYDQNEHRSGLKQWIEEAGGG 263

Query: 262 TVTAFDFTTKGILQAAVQGELWRLKDSNGKPPGLIGMKPESAVTFIDNHDTGSTQRIWPF 321
            +TAFDFTTKGILQ+AV+GELWRLKDS GKPPG+IG+ P +AVTFIDNHD   T R W F
Sbjct: 264 VLTAFDFTTKGILQSAVKGELWRLKDSQGKPPGMIGIMPGNAVTFIDNHD---TFRTWVF 323

Query: 322 PSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKLSEIRARNGIEKTSTVNILASDG 381
           PSDKV+ GY YILTHPGTP IFY+H+ +WGLKE ISKL  IR +NGI  TS+V I A++ 
Sbjct: 324 PSDKVLLGYVYILTHPGTPCIFYNHYIEWGLKESISKLVAIRNKNGIGSTSSVTIKAAEA 383

Query: 382 DLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEYAVWEKK 424
           DLY+A +D+K+IMKIGP  ++G L+PS++ LA SG+++AVWEKK
Sbjct: 384 DLYLAMIDDKVIMKIGPKQDVGTLVPSNFALAYSGLDFAVWEKK 423

[Close]

BLAST of Tan0022707 vs. TAIR 10
Match: AT1G69830.1 (alpha-amylase-like 3 )

HSP 1 Score: 391.7 bits (1005), Expect = 7.3e-109
Identity = 192/402 (47.76%), Postives = 253/402 (62.94%), Query Frame = 0


Query: 21  SSSTLLFQGFNWESSSKGGWYNSLKNIIPDLENARITHVWLPPPSQSAASQGYLPGRLYD 80
           S   +L QGFNWES+  G WY  L+    +L +   T +WLPPP++S + +GY+P  LY+
Sbjct: 493 SGFEILCQGFNWESNKSGRWYLELQEKADELASLGFTVLWLPPPTESVSPEGYMPKDLYN 552

Query: 81  LNASKYGTQDELKSLISALHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTSDSRLDW 140
           LN S+YGT DELK  +   H+ GIK + D V+NHR A  ++Q G+W +F G     RL+W
Sbjct: 553 LN-SRYGTIDELKDTVKKFHKVGIKVLGDAVLNHRCAHFKNQNGVWNLFGG-----RLNW 612

Query: 141 GPSFICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQVQNEISEWMRWLKTEIGFDGWRF 200
               + +DD  +  G GN+ SG+ +  AP+IDH    V+ +I EW+ W+  E+G+DGWR 
Sbjct: 613 DDRAVVADDPHF-QGRGNKSSGDNFHAAPNIDHSQDFVRKDIKEWLCWMMEEVGYDGWRL 672

Query: 201 DFVKGYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRHALADWVRASGDT 260
           DFV+G+     K YMD + P FAVGE WDSLSY   G+ + NQDAHR  + DW+ A+   
Sbjct: 673 DFVRGFWGGYVKDYMDASKPYFAVGEYWDSLSYTY-GEMDYNQDAHRQRIVDWINATSGA 732

Query: 261 VTAFDFTTKGILQAAVQG-ELWRLKDSNGKPPGLIGMKPESAVTFIDNHDTGSTQRIWPF 320
             AFD TTKGIL  A+Q  E WRL D  GKPPG++G  P  AVTFI+NHDTGSTQ  W F
Sbjct: 733 AGAFDVTTKGILHTALQKCEYWRLSDPKGKPPGVVGWWPSRAVTFIENHDTGSTQGHWRF 792

Query: 321 PSDKVMQGYAYILTHPGTPSIFYDHFFDWGLKEEISKLSEIRARNGIEKTSTVNILASDG 380
           P  K MQGYAYILTHPGTP++F+DH F      EI+ L  +R R  +   S VNI  S+ 
Sbjct: 793 PEGKEMQGYAYILTHPGTPAVFFDHIFS-DYHSEIAALLSLRNRQKLHCRSEVNIDKSER 852

Query: 381 DLYVAGVDEKIIMKIGPNTNLGNLIPSDYELATSGMEYAVWE 422
           D+Y A +DEK+ MKIGP          ++ +A  G +Y VWE
Sbjct: 853 DVYAAIIDEKVAMKIGPGHYEPPNGSQNWSVAVEGRDYKVWE 885

[Close]

BLAST of Tan0022707 vs. TAIR 10
Match: AT1G76130.1 (alpha-amylase-like 2 )

HSP 1 Score: 382.1 bits (980), Expect = 5.8e-106
Identity = 190/402 (47.26%), Postives = 256/402 (63.68%), Query Frame = 0


Query: 25  LLFQGFNWESSSKGGWYNSLKNIIPDLENARITHVWLPPPSQSAASQGYLPGRLYDLNAS 84
           ++ Q +NWE S K  W+ +L   +PD+  +  T  WLPPPSQS A +GYLP  LY LN S
Sbjct: 27  VILQAYNWE-SHKYDWWRNLDGKVPDIAKSGFTSAWLPPPSQSLAPEGYLPQDLYSLN-S 86

Query: 85  KYGTQDELKSLISALHEKGIKAIADIVINHRTAEKQDQRGIWCIFEGGTSDSRLDWGPSF 144
            YG++  LKSL+  + +  ++A+ADIVINHR    +   G++  ++G      L W    
Sbjct: 87  AYGSEHLLKSLLRKMKQYKVRAMADIVINHRVGTTRGHGGMYNRYDG----ISLPWDEHA 146

Query: 145 ICSDDTDYSDGTGNRDSGEPYPPAPDIDHLNPQVQNEISEWMRWLKTEIGFDGWRFDFVK 204
           +    T  + G GNR +G+ +   P++DH    V+ +I  W+RWL+  +GF  +RFDF +
Sbjct: 147 V----TSCTGGLGNRSTGDNFNGVPNVDHTQHFVRKDIIGWLRWLRNTVGFQDFRFDFAR 206

Query: 205 GYAPSITKLYMDRTSPDFAVGEKWDSLSYGQDGKPNQNQDAHRHALADWVRASGDTVTAF 264
           GY+ +  K Y+    P F+VGE WDS +Y   G  + NQD+HR  +  W+ A+G    AF
Sbjct: 207 GYSANYVKEYIGAAKPLFSVGECWDSCNYNGHGL-DYNQDSHRQRIISWIDATGQISAAF 266

Query: 265 DFTTKGILQAAVQGELWRLKDSNGKPPGLIGMKPESAVTFIDNHDTGSTQRIWPFPSDKV 324
           DFTTKGILQ AV+G+ WRL D+ GKPPG++G  P  AVTF+DNHDTGSTQ  WPFPS  V
Sbjct: 267 DFTTKGILQEAVKGQYWRLCDAQGKPPGVMGWWPSRAVTFLDNHDTGSTQAHWPFPSHHV 326

Query: 325 MQGYAYILTHPGTPSIFYDHFFDWG--LKEEISKLSEIRARNGIEKTSTVNILASDGDLY 384
           M+GYAYILTHPG P +FYDHF+DWG  + ++I KL +IR R  I   STV +L ++ +LY
Sbjct: 327 MEGYAYILTHPGIPCVFYDHFYDWGSSIHDQIVKLIDIRRRQDIHSRSTVRVLKAESNLY 386

Query: 385 VAGVDEKIIMKIGPNTNLGNLIPS--DYELATSGMEYAVWEK 423
            A V EKI MK+G     G+  PS  D+ LATSG  YAVW K
Sbjct: 387 AAIVGEKICMKLGD----GSWCPSGRDWTLATSGHRYAVWHK 413

The following BLAST results are available for this feature:

BLAST of Tan0022707 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P17859	5.0e-203	77.67	Alpha-amylase OS=Vigna mungo OX=3915 GN=AMY1.1 PE=2 SV=1	[more]
P27933	3.5e-164	61.88	Alpha-amylase isozyme 3D OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.3 PE=2...	[more]
Q8VZ56	5.0e-163	66.83	Alpha-amylase 1 OS=Arabidopsis thaliana OX=3702 GN=AMY1 PE=1 SV=1	[more]
P04063	8.6e-163	63.10	Alpha-amylase type B isozyme OS=Hordeum vulgare OX=4513 GN=AMY1.2 PE=1 SV=3	[more]
P27934	1.9e-162	60.91	Alpha-amylase isozyme 3E OS=Oryza sativa subsp. japonica OX=39947 GN=AMY1.4 PE=2...	[more]

BLAST of Tan0022707 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038896126.1	4.3e-228	89.15	alpha-amylase-like [Benincasa hispida]	[more]
XP_022142476.1	9.6e-228	89.13	alpha-amylase [Momordica charantia]	[more]
XP_022936258.1	1.9e-223	87.29	alpha-amylase, partial [Cucurbita moschata]	[more]
XP_022976296.1	2.4e-223	87.29	alpha-amylase [Cucurbita maxima]	[more]
XP_023536126.1	3.2e-223	87.29	alpha-amylase, partial [Cucurbita pepo subsp. pepo]	[more]

BLAST of Tan0022707 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CMA4	4.6e-228	89.13	Alpha-amylase OS=Momordica charantia OX=3673 GN=LOC111012587 PE=3 SV=1	[more]
A0A6J1FCS4	9.0e-224	87.29	Alpha-amylase OS=Cucurbita moschata OX=3662 GN=LOC111442931 PE=3 SV=1	[more]
A0A6J1IGI6	1.2e-223	87.29	Alpha-amylase OS=Cucurbita maxima OX=3661 GN=LOC111476734 PE=3 SV=1	[more]
A0A2I4EXA7	6.5e-206	79.67	Alpha-amylase OS=Juglans regia OX=51240 GN=LOC108993530 PE=3 SV=1	[more]
A0A2N9FNN7	1.2e-204	78.72	Alpha-amylase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS20239 PE=3 SV=1	[more]

BLAST of Tan0022707 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25000.1	3.6e-164	66.83	alpha-amylase-like	[more]
AT1G69830.1	7.3e-109	47.76	alpha-amylase-like 3	[more]
AT1G76130.1	5.8e-106	47.26	alpha-amylase-like 2	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006046	Alpha amylase	PRINTS	PR00110	ALPHAAMYLASE	coord: 103..114 score: 49.51 coord: 299..311 score: 51.43 coord: 195..206 score: 40.85
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	SMART	SM00642	aamy	coord: 24..349 e-value: 2.0E-46 score: 170.2
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	PFAM	PF00128	Alpha-amylase	coord: 55..208 e-value: 1.3E-15 score: 57.8
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 24..349 e-value: 4.3E-135 score: 452.6
None	No IPR available	PIRSR	PIRSR001021-2	PIRSR001021-2	coord: 169..333 e-value: 8.1E-12 score: 42.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 151..172
None	No IPR available	PANTHER	PTHR43447	ALPHA-AMYLASE	coord: 1..342
None	No IPR available	PANTHER	PTHR43447:SF37	ALPHA-AMYLASE 1	coord: 1..342
None	No IPR available	CDD	cd11314	AmyAc_arch_bac_plant_AmyA	coord: 26..340 e-value: 1.80884E-152 score: 429.333
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 25..342

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0022707.1	Tan0022707.1	mRNA
Tan0022707.2	Tan0022707.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0036261	7-methylguanosine cap hypermethylation
biological_process	GO:0009452	7-methylguanosine RNA capping
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005634	nucleus
molecular_function	GO:0004556	alpha-amylase activity
molecular_function	GO:0103025	alpha-amylase activity (releasing maltohexaose)
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0071164	RNA trimethylguanosine synthase activity
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0043169	cation binding