Tan0022699 (gene) Snake gourd v1

Overview


Name	Tan0022699
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Membrane-associated kinase regulator 5
Location	LG07: 7720417 .. 7722294 (-)
RNA-Seq Expression	Tan0022699
Synteny	Tan0022699

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGGAGACTTTCAAAAAATAAAAAAAAATAATAAAAAAAAAGCTTACATCACCTTCTCTGTTTCCTCCCAAAGTTCCCAAACACCACTGTAAACCAAAATCCCTCCATTGAAGAAACCAACCCCAAATCTCTCTCTATAAAACCCACCAAAAACCCACGATTGTGTTCCTCTCCTCTCTCCTCCAACCATGGATTCCGTCGCCTTTCTCAAGTTGTTCAAACTTTCCGTCGCCGATGAACAGAAAAAACAACAGAAGCAAGAACAAGATGAAGAAGAAGAAGATTCGTTCTTCGATCTCGAGCTTCTCTCTGTTTCCGCCGGCGATATTCAAAGCCCAGAACAGATACCTCAAATTCCGACCAAATTAGGCGACCACATAAACCTCTGTTCCGATTCCATTTCCAAACGGAAGATTCTTCCAATCGAGCCCACTTCCAAGCCACAATCGCCGATTCAGCTTCTCAAATCGGCCCCCAAGTTCCCTGTTTTCATCTTCAAGAAACAGAGATCAATGGCCAAAAACAGAGCACCTGCCGGAGCAGAACAAGACGAAAGCAGAGTTTTGAACTCGAAGCTTTCCAGAGACAACAGCACGAGGAGAATCGGAGGCATATTTCCGTCCGACCCACGTGGGGCTCCGACGACGACGACGGCGACGGCGAGGCGGTTCTCGAAGGAGGCAGTGCAGAAGTATTTGAGACTGATTAAGCCTAAGGTTCTGAAGAGACCCACCGACAACAGCGACGATCTGTCGGCGGCGGCGGTGGATTCTCCGGTGAGGGAGAAGCACGAGAATGGTCTGCCGGCGGGGATTCGGTTGGTCTGTAAGCATCTGGGTAAGAGCAAATCAGCATCGGCTGCTGGCGGATTAGCGGTGGCGCCACCGTTGAACCGGAGAGATGATTCGTTGTTGCAGCAGCACGACGGAATTCAAAGCGCCATTTTACATTGTAAAAGATCCTTCAACGCTTCCACAGGTAAAAACTTCAAAAAAAAAAAAAAAAAAAGTCTCAAAGCTTTCCATTTTAGCATCTAATAACTCTCTAATTTTTTTTTAAAAAAAATTTGTATTTAACAAGTCTTTACATTTCATTTTAAAATTTTGAAAAGTATACAACTTTGCCTTCCACGTTCTTGAACCAGACAAAATTCAAACGGGTTATTTAGTGAAAGACATTTATAACAATTTTTTTTTTTTAAAAAAGTGAGTAATGTTGGATTTATTATTGTGGTCAGATAAATCTGTGTTGTTCTCAAGGCCAGAAACAGCAGCCTCACAAGAAAGGTCAAGAAGTCTATCTTGCAAAGGTATATTACTTGTTTTCCCAATTTCCAGTTCCACTAGAGCTTAAAATAAACAAATCAGATTATGATTTGGTTTTTTTTTTTTTTTTTTTTTTTGACAGATTTTTGTTTCAAAGAAAGCAAAGAAGAAGGGAATTGAGAGCAACCCAGAAGAGAAAGAACAGTGAAATGAAAGAGAAATATATATATATATATATATTTTGAGGGATTTGTTTGTAAAGGAAGGGGGAATTTTTGGGTGATGAAAAGTGACAATGAAGAAGAAGAAGAAGAAATGGGTTTGATTTTGAGAGTAGGGAGATGTGTAAAGGGTTATGAAGGAATTTGAAAAGAAATCTGATTGTGAAATCTGCCTTTGTGTTGGGATTTTGTCTGATGCAAACTACAACCATTAACCAAAAAACAAATGGACATCTTTTTTCTTTTTTCTTTTTTTAATTTTATTATATATTCAGTGTTTGTTTTTTTTTTTCTCAGAAACAGTGTAATATGTAATATGTAGTTTCAAAAAGTTTAGTTGTATAATATTGGAGCTTTTTTTTTTTCTTTTTTAAGTTGAACAAACATGTGGG

mRNA sequence

TGGGAGACTTTCAAAAAATAAAAAAAAATAATAAAAAAAAAGCTTACATCACCTTCTCTGTTTCCTCCCAAAGTTCCCAAACACCACTGTAAACCAAAATCCCTCCATTGAAGAAACCAACCCCAAATCTCTCTCTATAAAACCCACCAAAAACCCACGATTGTGTTCCTCTCCTCTCTCCTCCAACCATGGATTCCGTCGCCTTTCTCAAGTTGTTCAAACTTTCCGTCGCCGATGAACAGAAAAAACAACAGAAGCAAGAACAAGATGAAGAAGAAGAAGATTCGTTCTTCGATCTCGAGCTTCTCTCTGTTTCCGCCGGCGATATTCAAAGCCCAGAACAGATACCTCAAATTCCGACCAAATTAGGCGACCACATAAACCTCTGTTCCGATTCCATTTCCAAACGGAAGATTCTTCCAATCGAGCCCACTTCCAAGCCACAATCGCCGATTCAGCTTCTCAAATCGGCCCCCAAGTTCCCTGTTTTCATCTTCAAGAAACAGAGATCAATGGCCAAAAACAGAGCACCTGCCGGAGCAGAACAAGACGAAAGCAGAGTTTTGAACTCGAAGCTTTCCAGAGACAACAGCACGAGGAGAATCGGAGGCATATTTCCGTCCGACCCACGTGGGGCTCCGACGACGACGACGGCGACGGCGAGGCGGTTCTCGAAGGAGGCAGTGCAGAAGTATTTGAGACTGATTAAGCCTAAGGTTCTGAAGAGACCCACCGACAACAGCGACGATCTGTCGGCGGCGGCGGTGGATTCTCCGGTGAGGGAGAAGCACGAGAATGGTCTGCCGGCGGGGATTCGGTTGGTCTGTAAGCATCTGGGTAAGAGCAAATCAGCATCGGCTGCTGGCGGATTAGCGGTGGCGCCACCGTTGAACCGGAGAGATGATTCGTTGTTGCAGCAGCACGACGGAATTCAAAGCGCCATTTTACATTGTAAAAGATCCTTCAACGCTTCCACAGATAAATCTGTGTTGTTCTCAAGGCCAGAAACAGCAGCCTCACAAGAAAGGTCAAGAAGTCTATCTTGCAAAGATTTTTGTTTCAAAGAAAGCAAAGAAGAAGGGAATTGAGAGCAACCCAGAAGAGAAAGAACAGTGAAATGAAAGAGAAATATATATATATATATATATTTTGAGGGATTTGTTTGTAAAGGAAGGGGGAATTTTTGGGTGATGAAAAGTGACAATGAAGAAGAAGAAGAAGAAATGGGTTTGATTTTGAGAGTAGGGAGATGTGTAAAGGGTTATGAAGGAATTTGAAAAGAAATCTGATTGTGAAATCTGCCTTTGTGTTGGGATTTTGTCTGATGCAAACTACAACCATTAACCAAAAAACAAATGGACATCTTTTTTCTTTTTTCTTTTTTTAATTTTATTATATATTCAGTGTTTGTTTTTTTTTTTCTCAGAAACAGTGTAATATGTAATATGTAGTTTCAAAAAGTTTAGTTGTATAATATTGGAGCTTTTTTTTTTTCTTTTTTAAGTTGAACAAACATGTGGG

Coding sequence (CDS)

ATGGATTCCGTCGCCTTTCTCAAGTTGTTCAAACTTTCCGTCGCCGATGAACAGAAAAAACAACAGAAGCAAGAACAAGATGAAGAAGAAGAAGATTCGTTCTTCGATCTCGAGCTTCTCTCTGTTTCCGCCGGCGATATTCAAAGCCCAGAACAGATACCTCAAATTCCGACCAAATTAGGCGACCACATAAACCTCTGTTCCGATTCCATTTCCAAACGGAAGATTCTTCCAATCGAGCCCACTTCCAAGCCACAATCGCCGATTCAGCTTCTCAAATCGGCCCCCAAGTTCCCTGTTTTCATCTTCAAGAAACAGAGATCAATGGCCAAAAACAGAGCACCTGCCGGAGCAGAACAAGACGAAAGCAGAGTTTTGAACTCGAAGCTTTCCAGAGACAACAGCACGAGGAGAATCGGAGGCATATTTCCGTCCGACCCACGTGGGGCTCCGACGACGACGACGGCGACGGCGAGGCGGTTCTCGAAGGAGGCAGTGCAGAAGTATTTGAGACTGATTAAGCCTAAGGTTCTGAAGAGACCCACCGACAACAGCGACGATCTGTCGGCGGCGGCGGTGGATTCTCCGGTGAGGGAGAAGCACGAGAATGGTCTGCCGGCGGGGATTCGGTTGGTCTGTAAGCATCTGGGTAAGAGCAAATCAGCATCGGCTGCTGGCGGATTAGCGGTGGCGCCACCGTTGAACCGGAGAGATGATTCGTTGTTGCAGCAGCACGACGGAATTCAAAGCGCCATTTTACATTGTAAAAGATCCTTCAACGCTTCCACAGATAAATCTGTGTTGTTCTCAAGGCCAGAAACAGCAGCCTCACAAGAAAGGTCAAGAAGTCTATCTTGCAAAGATTTTTGTTTCAAAGAAAGCAAAGAAGAAGGGAATTGA

Protein sequence

MDSVAFLKLFKLSVADEQKKQQKQEQDEEEEDSFFDLELLSVSAGDIQSPEQIPQIPTKLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNRAPAGAEQDESRVLNSKLSRDNSTRRIGGIFPSDPRGAPTTTTATARRFSKEAVQKYLRLIKPKVLKRPTDNSDDLSAAAVDSPVREKHENGLPAGIRLVCKHLGKSKSASAAGGLAVAPPLNRRDDSLLQQHDGIQSAILHCKRSFNASTDKSVLFSRPETAASQERSRSLSCKDFCFKESKEEGN

Homology

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BLAST of Tan0022699 vs. ExPASy Swiss-Prot
Match: Q9FLX4 (Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 PE=2 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 1.0e-24
Identity = 98/269 (36.43%), Postives = 139/269 (51.67%), Query Frame = 0


Query: 29  EEEDSFFDLELLSVSAGDIQSPEQIPQIPTKLGDHINLCSDSISKRKILP-IEPTSKP-Q 88
           E EDSFF+L+   +S  D ++P++  ++ T         S   +K K+LP ++ +SKP Q
Sbjct: 45  EGEDSFFELK---ISLSDFKTPKEKQRLETTTTTTTYSVS---NKSKVLPFVDISSKPQQ 104

Query: 89  SPIQLLKSAPKFPVFIFKKQRSMAKNRAPAGAEQDESRVLNSKLSRDNST-----RRIGG 148
           SP  LLKS  KF  F FKK  S          E++    LN +   D+ T     R+   
Sbjct: 105 SPTTLLKSGQKFRAFSFKK--SEKSTTTEKKKEENNRTSLNVRFRVDDETTTTSFRKTAS 164

Query: 149 IFPSDPRGAPTTTTATARRFSKEAVQKYLRLIKPKVLKRPTDNSDDLSAAAVDSP-VREK 208
           I     R   T  T     F     +++  LIKP   K     S     +   SP  REK
Sbjct: 165 I----ARSQQTDDT----MFDDSVSKRFFSLIKPLYTKSTKKQSSSTITSPTSSPATREK 224

Query: 209 HENGLPAGIRLVCKHLGKSKSASAA-GGLAVAPPLNRRDDSLLQQHDGIQSAILHCKRSF 268
             + +P+G+R V + LGKS+SASAA GG++   P NR D+SL  Q DGIQSAILHCK+SF
Sbjct: 225 QRSNIPSGMRSVRRQLGKSRSASAAIGGMS---PANRIDESLQVQQDGIQSAILHCKKSF 284

Query: 269 NASTDKSVLFSRPETAASQERSRSLSCKD 289
           + S + S+L      ++SQE+  + S +D
Sbjct: 285 HGSRESSLLSRSTSESSSQEKLSTSSSED 294

[Close]

BLAST of Tan0022699 vs. ExPASy Swiss-Prot
Match: Q9SH58 (Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 GN=MAKR2 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.6e-14
Identity = 67/217 (30.88%), Postives = 101/217 (46.54%), Query Frame = 0


Query: 77  LPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNRAPAGAEQDESRVLNSKLSRDNST 136
           L + P S+ +S + +     + P+     + + +KN + + +    S   +S L + N  
Sbjct: 198 LSLSPESQQKSTVTVNLKVEEVPIISLFSRENSSKNSSSSSSSSSSS---SSSLKKQNGN 257

Query: 137 RRIGGIFPSDPRGAPTTTTATARRFSKEAVQKYLRLIKP---KVLKR-----------PT 196
             +                +  +RF    +QKYL+ +KP   +V +R             
Sbjct: 258 ESV---------------VSDEKRF--VMMQKYLKKVKPLYIRVSRRYGEKLRHSGQLTA 317

Query: 197 DNSDDLSAAAVDSPVREKHEN------GLPAGIRLVCKHLGKSKSASA--------AGGL 256
            +S   +A   DSPV  K  N       +PAG+++V KHLGKS+S+S+           +
Sbjct: 318 PSSARSTAEKADSPVATKTNNKPGNNINIPAGLKVVRKHLGKSRSSSSTATTPSSTTATV 377

Query: 257 AVAPPLNRRDDSLLQQHDGIQSAILHCKRSFNASTDK 266
                  RRDDSLLQQ D IQSAILHCKRSFN+S DK
Sbjct: 378 TTQSESRRRDDSLLQQQDSIQSAILHCKRSFNSSRDK 394

[Close]

BLAST of Tan0022699 vs. NCBI nr
Match: KAG6605748.1 (putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 479.6 bits (1233), Expect = 2.0e-131
Identity = 258/299 (86.29%), Postives = 270/299 (90.30%), Query Frame = 0


Query: 1   MDSVAFLKLFKLSVADEQKKQQKQEQD-EEEEDSFFDLELLSVSAGDIQSPEQIPQIPTK 60
           MDS+AF +LFKLSVADE   QQK+EQD EEEEDSFFDLE LSVSAGDI SPEQ PQIPTK
Sbjct: 1   MDSLAFRRLFKLSVADE---QQKREQDGEEEEDSFFDLEFLSVSAGDIHSPEQRPQIPTK 60

Query: 61  LGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNRAPAGAE 120
           LG+HINLCSDSISKRKILPIEPTSKP SPIQLLKSAPKFPV IF KQRSMAKNRAP G E
Sbjct: 61  LGEHINLCSDSISKRKILPIEPTSKPHSPIQLLKSAPKFPVSIFNKQRSMAKNRAPGGTE 120

Query: 121 QDESRVLNSKLSRDNSTRRIGGIFPSDPRGAPTTTTATARRFSKEAVQKYLRLIKPKVLK 180
           Q E RVL+S LSRDNSTRRIGG+FP DP  AP TTTATARRFSKEAVQKYLRLIKPKVLK
Sbjct: 121 QVERRVLSSNLSRDNSTRRIGGVFPVDPCEAPATTTATARRFSKEAVQKYLRLIKPKVLK 180

Query: 181 RPTDNSDDLSAAAVDSPVREKHENGLPAGIRLVCKHLGKSKSASAAGGLAVAPPLNRRDD 240
           +PTD SD+LS   VDSP REKH+NGLPAGIRLVCKHLGKSKSASA GGLAVAPPLNRRDD
Sbjct: 181 KPTDYSDNLS-TTVDSPAREKHDNGLPAGIRLVCKHLGKSKSASATGGLAVAPPLNRRDD 240

Query: 241 SLLQQHDGIQSAILHCKRSFNASTDKSVLFSRPETAASQERSRSLSCKDFCFKESKEEG 299
           SLLQQHDGIQSAILHCKRSFNAST+K +LFSR ETAASQERSRSLSCKDF F+ESKEEG
Sbjct: 241 SLLQQHDGIQSAILHCKRSFNASTEKPLLFSRSETAASQERSRSLSCKDFSFRESKEEG 295

[Close]

BLAST of Tan0022699 vs. NCBI nr
Match: XP_022996006.1 (probable membrane-associated kinase regulator 5 [Cucurbita maxima])

HSP 1 Score: 473.0 bits (1216), Expect = 1.9e-129
Identity = 255/299 (85.28%), Postives = 269/299 (89.97%), Query Frame = 0


Query: 1   MDSVAFLKLFKLSVADEQKKQQKQEQD-EEEEDSFFDLELLSVSAGDIQSPEQIPQIPTK 60
           MDS+AF +LFKLSVADE   QQK+EQD EEEEDSFFDLE LSVSAGDI SPEQ PQIPTK
Sbjct: 1   MDSLAFRRLFKLSVADE---QQKREQDGEEEEDSFFDLEFLSVSAGDIHSPEQTPQIPTK 60

Query: 61  LGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNRAPAGAE 120
           LG+HINLCSDSISKRKILPIEPTSKP SPIQLLKSAPKFPV IF KQRSMAKNRA  G E
Sbjct: 61  LGEHINLCSDSISKRKILPIEPTSKPHSPIQLLKSAPKFPVSIFNKQRSMAKNRASGGTE 120

Query: 121 QDESRVLNSKLSRDNSTRRIGGIFPSDPRGAPTTTTATARRFSKEAVQKYLRLIKPKVLK 180
           Q E RVL+S LSRDNSTRRIGGIFP+DP   P TTTATARRFSKEAVQKYLRLIKPKVLK
Sbjct: 121 QVERRVLSSNLSRDNSTRRIGGIFPADPCEDPATTTATARRFSKEAVQKYLRLIKPKVLK 180

Query: 181 RPTDNSDDLSAAAVDSPVREKHENGLPAGIRLVCKHLGKSKSASAAGGLAVAPPLNRRDD 240
           +PT  SD+LS   VDSP R+KH+NGLPAGIRLVCKHLGKSKSASA+GGLAVAPPLNRRDD
Sbjct: 181 KPTGYSDNLS-TTVDSPARDKHDNGLPAGIRLVCKHLGKSKSASASGGLAVAPPLNRRDD 240

Query: 241 SLLQQHDGIQSAILHCKRSFNASTDKSVLFSRPETAASQERSRSLSCKDFCFKESKEEG 299
           SLLQQHDGIQSAILHCKRSFNAST+K +LFSR ETAASQERSRSLSCKDF F+ESKEEG
Sbjct: 241 SLLQQHDGIQSAILHCKRSFNASTEKPLLFSRSETAASQERSRSLSCKDFSFRESKEEG 295

[Close]

BLAST of Tan0022699 vs. NCBI nr
Match: XP_023534292.1 (probable membrane-associated kinase regulator 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 472.2 bits (1214), Expect = 3.2e-129
Identity = 254/299 (84.95%), Postives = 269/299 (89.97%), Query Frame = 0


Query: 1   MDSVAFLKLFKLSVADEQKKQQKQEQD-EEEEDSFFDLELLSVSAGDIQSPEQIPQIPTK 60
           MDS+AF +LFKLSVADE   QQK+EQD +EEEDSFFDLE LSVSAGDI SPEQ PQIPTK
Sbjct: 1   MDSLAFRRLFKLSVADE---QQKREQDGDEEEDSFFDLEFLSVSAGDIHSPEQRPQIPTK 60

Query: 61  LGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNRAPAGAE 120
           LG+HINLCSDSISKRKILP+EPTSKP SPIQLLKSAPKFPV IF KQRSMAKNRAP G E
Sbjct: 61  LGEHINLCSDSISKRKILPMEPTSKPHSPIQLLKSAPKFPVSIFNKQRSMAKNRAPGGTE 120

Query: 121 QDESRVLNSKLSRDNSTRRIGGIFPSDPRGAPTTTTATARRFSKEAVQKYLRLIKPKVLK 180
           Q E RVL+S LSRDNSTRRIGG+FP+DP  AP TTT TARRFSKEAVQKYLRLIKPKVLK
Sbjct: 121 QVERRVLSSNLSRDNSTRRIGGVFPADPCEAPATTTTTARRFSKEAVQKYLRLIKPKVLK 180

Query: 181 RPTDNSDDLSAAAVDSPVREKHENGLPAGIRLVCKHLGKSKSASAAGGLAVAPPLNRRDD 240
           +PTD SD+LS   VDSP REKH+NGLPAGIRLV KHLGKSKSASA GGLAVAPPLNRRDD
Sbjct: 181 KPTDYSDNLS-TTVDSPAREKHDNGLPAGIRLVGKHLGKSKSASATGGLAVAPPLNRRDD 240

Query: 241 SLLQQHDGIQSAILHCKRSFNASTDKSVLFSRPETAASQERSRSLSCKDFCFKESKEEG 299
           SLLQQHDGIQSAILHCKRSFNAST+K +LFSR ETAASQERSRSLSCKDF F+ESKEEG
Sbjct: 241 SLLQQHDGIQSAILHCKRSFNASTEKPLLFSRSETAASQERSRSLSCKDFSFRESKEEG 295

[Close]

BLAST of Tan0022699 vs. NCBI nr
Match: XP_022958606.1 (probable membrane-associated kinase regulator 5 [Cucurbita moschata])

HSP 1 Score: 468.8 bits (1205), Expect = 3.6e-128
Identity = 255/299 (85.28%), Postives = 267/299 (89.30%), Query Frame = 0


Query: 1   MDSVAFLKLFKLSVADEQKKQQKQEQD-EEEEDSFFDLELLSVSAGDIQSPEQIPQIPTK 60
           MDS+AF +LFKLSVADE   QQK+EQD EEEEDSFFDLE LSVSAGDI SPEQ PQIPTK
Sbjct: 31  MDSLAFRRLFKLSVADE---QQKREQDGEEEEDSFFDLEFLSVSAGDIHSPEQRPQIPTK 90

Query: 61  LGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNRAPAGAE 120
           LG+HINLCSDSISKRKILPIEPTSKP SPIQLLKSAPKFPV IF KQRSMAKNRAP G E
Sbjct: 91  LGEHINLCSDSISKRKILPIEPTSKPHSPIQLLKSAPKFPVSIFNKQRSMAKNRAPGGTE 150

Query: 121 QDESRVLNSKLSRDNSTRRIGGIFPSDPRGAPTTTTATARRFSKEAVQKYLRLIKPKVLK 180
           Q E RVL+S LSRDNSTRRIGG+FP DP  AP T   TARRFSKEAVQKYLRLIKPKVLK
Sbjct: 151 QVERRVLSSNLSRDNSTRRIGGVFPVDPCEAPAT---TARRFSKEAVQKYLRLIKPKVLK 210

Query: 181 RPTDNSDDLSAAAVDSPVREKHENGLPAGIRLVCKHLGKSKSASAAGGLAVAPPLNRRDD 240
           +PTD SD+LS   VDSP REKH+NGLPAGIRLVCKHLGKSKSASA GGLAVAPPLNRRDD
Sbjct: 211 KPTDYSDNLS-TTVDSPAREKHDNGLPAGIRLVCKHLGKSKSASATGGLAVAPPLNRRDD 270

Query: 241 SLLQQHDGIQSAILHCKRSFNASTDKSVLFSRPETAASQERSRSLSCKDFCFKESKEEG 299
           SLLQQHDGIQSAILHCKRSFNAST+K +LFSR ETAASQERSRSLSCKDF F+ESKEEG
Sbjct: 271 SLLQQHDGIQSAILHCKRSFNASTEKPLLFSRSETAASQERSRSLSCKDFSFRESKEEG 322

[Close]

BLAST of Tan0022699 vs. NCBI nr
Match: XP_038906856.1 (probable membrane-associated kinase regulator 5 [Benincasa hispida])

HSP 1 Score: 456.4 bits (1173), Expect = 1.8e-124
Identity = 250/305 (81.97%), Postives = 265/305 (86.89%), Query Frame = 0


Query: 1   MDSVAFLKLFKLSVADEQKKQQKQEQDEEEEDSFFDLELLSVSAGDIQSPEQIPQIPTKL 60
           MDSVAFLKLFKLS ADE +K     ++EEEEDSFFDLE  SVSAG IQSPEQ PQIPTKL
Sbjct: 1   MDSVAFLKLFKLSGADELQK----HEEEEEEDSFFDLEFFSVSAGKIQSPEQKPQIPTKL 60

Query: 61  GDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNRAPAGAEQ 120
           GDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRS+AKNRAPAG E+
Sbjct: 61  GDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSVAKNRAPAGTEE 120

Query: 121 DE-SRVLNSKLSRDNSTRRIGGIFPSDPRGAPTTTTATARRFSKEAVQKYLRLIKPKVLK 180
           +E SRVL+SKLSRDNSTRRIGGIFP DPR  P    A  RRFSKEAVQKYL+LIKPKVL+
Sbjct: 121 EEGSRVLSSKLSRDNSTRRIGGIFPLDPREPP--VPAPTRRFSKEAVQKYLKLIKPKVLR 180

Query: 181 RPTDNSDDLSAAAVDSPVREKHENGLPAGIRLVCKHLGKSKSASAAGGL-----AVAPPL 240
           +PT +  D S+AAVDSPVREKHENGLPAGIRLVC+HLGKSKSASAAGGL     AV PP 
Sbjct: 181 KPTYSGGDFSSAAVDSPVREKHENGLPAGIRLVCRHLGKSKSASAAGGLAAAVSAVVPPP 240

Query: 241 NRRDDSLLQQHDGIQSAILHCKRSFNASTDKSVLFSRPETAASQERSRSLSCKDFCFKES 300
           NRRDDSLLQQHDGIQSAILHCKRSFNAS +K V  SR E+AASQER RSLSCKDF FKES
Sbjct: 241 NRRDDSLLQQHDGIQSAILHCKRSFNASAEKPVWLSRSESAASQERPRSLSCKDFSFKES 299

[Close]

BLAST of Tan0022699 vs. ExPASy TrEMBL
Match: A0A6J1K3H3 (probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=LOC111491347 PE=4 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 9.2e-130
Identity = 255/299 (85.28%), Postives = 269/299 (89.97%), Query Frame = 0


Query: 1   MDSVAFLKLFKLSVADEQKKQQKQEQD-EEEEDSFFDLELLSVSAGDIQSPEQIPQIPTK 60
           MDS+AF +LFKLSVADE   QQK+EQD EEEEDSFFDLE LSVSAGDI SPEQ PQIPTK
Sbjct: 1   MDSLAFRRLFKLSVADE---QQKREQDGEEEEDSFFDLEFLSVSAGDIHSPEQTPQIPTK 60

Query: 61  LGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNRAPAGAE 120
           LG+HINLCSDSISKRKILPIEPTSKP SPIQLLKSAPKFPV IF KQRSMAKNRA  G E
Sbjct: 61  LGEHINLCSDSISKRKILPIEPTSKPHSPIQLLKSAPKFPVSIFNKQRSMAKNRASGGTE 120

Query: 121 QDESRVLNSKLSRDNSTRRIGGIFPSDPRGAPTTTTATARRFSKEAVQKYLRLIKPKVLK 180
           Q E RVL+S LSRDNSTRRIGGIFP+DP   P TTTATARRFSKEAVQKYLRLIKPKVLK
Sbjct: 121 QVERRVLSSNLSRDNSTRRIGGIFPADPCEDPATTTATARRFSKEAVQKYLRLIKPKVLK 180

Query: 181 RPTDNSDDLSAAAVDSPVREKHENGLPAGIRLVCKHLGKSKSASAAGGLAVAPPLNRRDD 240
           +PT  SD+LS   VDSP R+KH+NGLPAGIRLVCKHLGKSKSASA+GGLAVAPPLNRRDD
Sbjct: 181 KPTGYSDNLS-TTVDSPARDKHDNGLPAGIRLVCKHLGKSKSASASGGLAVAPPLNRRDD 240

Query: 241 SLLQQHDGIQSAILHCKRSFNASTDKSVLFSRPETAASQERSRSLSCKDFCFKESKEEG 299
           SLLQQHDGIQSAILHCKRSFNAST+K +LFSR ETAASQERSRSLSCKDF F+ESKEEG
Sbjct: 241 SLLQQHDGIQSAILHCKRSFNASTEKPLLFSRSETAASQERSRSLSCKDFSFRESKEEG 295

[Close]

BLAST of Tan0022699 vs. ExPASy TrEMBL
Match: A0A6J1H2I5 (probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN=LOC111459782 PE=4 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 1.7e-128
Identity = 255/299 (85.28%), Postives = 267/299 (89.30%), Query Frame = 0


Query: 1   MDSVAFLKLFKLSVADEQKKQQKQEQD-EEEEDSFFDLELLSVSAGDIQSPEQIPQIPTK 60
           MDS+AF +LFKLSVADE   QQK+EQD EEEEDSFFDLE LSVSAGDI SPEQ PQIPTK
Sbjct: 31  MDSLAFRRLFKLSVADE---QQKREQDGEEEEDSFFDLEFLSVSAGDIHSPEQRPQIPTK 90

Query: 61  LGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNRAPAGAE 120
           LG+HINLCSDSISKRKILPIEPTSKP SPIQLLKSAPKFPV IF KQRSMAKNRAP G E
Sbjct: 91  LGEHINLCSDSISKRKILPIEPTSKPHSPIQLLKSAPKFPVSIFNKQRSMAKNRAPGGTE 150

Query: 121 QDESRVLNSKLSRDNSTRRIGGIFPSDPRGAPTTTTATARRFSKEAVQKYLRLIKPKVLK 180
           Q E RVL+S LSRDNSTRRIGG+FP DP  AP T   TARRFSKEAVQKYLRLIKPKVLK
Sbjct: 151 QVERRVLSSNLSRDNSTRRIGGVFPVDPCEAPAT---TARRFSKEAVQKYLRLIKPKVLK 210

Query: 181 RPTDNSDDLSAAAVDSPVREKHENGLPAGIRLVCKHLGKSKSASAAGGLAVAPPLNRRDD 240
           +PTD SD+LS   VDSP REKH+NGLPAGIRLVCKHLGKSKSASA GGLAVAPPLNRRDD
Sbjct: 211 KPTDYSDNLS-TTVDSPAREKHDNGLPAGIRLVCKHLGKSKSASATGGLAVAPPLNRRDD 270

Query: 241 SLLQQHDGIQSAILHCKRSFNASTDKSVLFSRPETAASQERSRSLSCKDFCFKESKEEG 299
           SLLQQHDGIQSAILHCKRSFNAST+K +LFSR ETAASQERSRSLSCKDF F+ESKEEG
Sbjct: 271 SLLQQHDGIQSAILHCKRSFNASTEKPLLFSRSETAASQERSRSLSCKDFSFRESKEEG 322

[Close]

BLAST of Tan0022699 vs. ExPASy TrEMBL
Match: A0A1S3AUW4 (probable membrane-associated kinase regulator 5 OS=Cucumis melo OX=3656 GN=LOC103482925 PE=4 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 2.7e-121
Identity = 244/308 (79.22%), Postives = 266/308 (86.36%), Query Frame = 0


Query: 1   MDSVAFLKLFKLSVADE---QKKQQKQEQDEEEEDSFFDLELLSVSAGDIQSPEQIPQIP 60
           MDSVAFLK FK S ADE   Q++++++E++EEEEDSFFD+E  SVSAG IQSPEQ PQIP
Sbjct: 1   MDSVAFLKFFKHSGADELQKQEEEEEEEEEEEEEDSFFDIEFFSVSAGKIQSPEQRPQIP 60

Query: 61  TKLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNRAPAG 120
           TKLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKN+AP G
Sbjct: 61  TKLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNKAPTG 120

Query: 121 AEQDESRVLNSKLSRDNSTRRIGGIFPSDPRGAPTTTTATARRFSKEAVQKYLRLIKPKV 180
            E D+S V +SKL+RDNSTRR GGIFPSDP   P    A  RRFSKEAV+KY +LIKPKV
Sbjct: 121 QEGDQSGVPSSKLTRDNSTRRFGGIFPSDPCEPP--VPAPTRRFSKEAVRKYFKLIKPKV 180

Query: 181 LKRPTDNSDDL---SAAAVDSPVREKHENGLPAGIRLVCKHLGKSKSASAAGGL--AVAP 240
           L++PTD+  DL   SAAAVDSPVREKHENGLPAGIRLVC+HLGKSKSASAAGGL  AV P
Sbjct: 181 LRKPTDSGSDLSSSSAAAVDSPVREKHENGLPAGIRLVCRHLGKSKSASAAGGLAAAVVP 240

Query: 241 PLNRRDDSLLQQHDGIQSAILHCKRSFNASTDKSV-LFSRPETAASQERSRSLSCKDFCF 300
           P NRRDDSLLQQHDGIQSAILHCKRSFNAS++K + L SR ET ASQER RSLSCKDF F
Sbjct: 241 PPNRRDDSLLQQHDGIQSAILHCKRSFNASSEKPLSLSSRSETTASQERPRSLSCKDFSF 300

[Close]

BLAST of Tan0022699 vs. ExPASy TrEMBL
Match: A0A0A0KK03 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139900 PE=4 SV=1)

HSP 1 Score: 443.4 bits (1139), Expect = 7.8e-121
Identity = 244/304 (80.26%), Postives = 261/304 (85.86%), Query Frame = 0


Query: 1   MDSVAFLKLFKLSVADEQKKQQKQEQDEEE--EDSFFDLELLSVSAGDIQSPEQIPQIPT 60
           MDSVAFLK FK S ADE +KQ+++E++EEE  EDSFFD+E  SVSAG IQSPEQ PQIPT
Sbjct: 1   MDSVAFLKFFKHSGADELQKQEEEEEEEEEEGEDSFFDIEFFSVSAGKIQSPEQRPQIPT 60

Query: 61  KLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNRAPAGA 120
           KLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNR P G 
Sbjct: 61  KLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNRPPTGQ 120

Query: 121 EQDESRVLNSKLSRDNSTRRIGGIFPSDPRGAPTTTTATARRFSKEAVQKYLRLIKPKVL 180
           E  ESRVLNSKL+RDNSTRR GGIFP DP   P    A  RRFSKE VQKYL+LIKPKVL
Sbjct: 121 EGGESRVLNSKLTRDNSTRRFGGIFPPDPCEPP--VPAPTRRFSKEVVQKYLKLIKPKVL 180

Query: 181 KRPTDNSDDLSAAAVDSPVREKHENGLPAGIRLVCKHLGKSKSASAAGGLAVA--PPLNR 240
           ++PTD+  DLS+AAVDSPVREKHENGLPAGIRLVC+HLGKSKSASAAGGLA A  P  NR
Sbjct: 181 RKPTDSGSDLSSAAVDSPVREKHENGLPAGIRLVCRHLGKSKSASAAGGLAAAVIPQTNR 240

Query: 241 RDDSLLQQHDGIQSAILHCKRSFNASTDKSV-LFSRPE-TAASQERSRSLSCKDFCFKES 299
           RDDSLLQQHDGIQSAILHCKRSFNAS++K + L SR E T ASQER RSLSCKDF FKE 
Sbjct: 241 RDDSLLQQHDGIQSAILHCKRSFNASSEKPLFLSSRSEATTASQERPRSLSCKDFSFKEG 300

[Close]

BLAST of Tan0022699 vs. ExPASy TrEMBL
Match: A0A5D3C3T3 (Putative membrane-associated kinase regulator 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00860 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 3.9e-120
Identity = 244/311 (78.46%), Postives = 263/311 (84.57%), Query Frame = 0


Query: 1   MDSVAFLKLFKLSVADEQKKQ------QKQEQDEEEEDSFFDLELLSVSAGDIQSPEQIP 60
           MDSVAFLK FK S ADE +KQ      +++E++EEEEDSFFD+E  SVSAG IQSPEQ P
Sbjct: 1   MDSVAFLKFFKHSGADELQKQEEEEEEEEEEEEEEEEDSFFDIEFFSVSAGKIQSPEQRP 60

Query: 61  QIPTKLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNRA 120
           QIPTKLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKN+A
Sbjct: 61  QIPTKLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNKA 120

Query: 121 PAGAEQDESRVLNSKLSRDNSTRRIGGIFPSDPRGAPTTTTATARRFSKEAVQKYLRLIK 180
           P   E D+S V +SKL+RDNSTRR GGIFPSDP   P    A  RRFSKEAV+KY +LIK
Sbjct: 121 PTAQEGDQSGVPSSKLTRDNSTRRFGGIFPSDPCEPP--VPAPTRRFSKEAVRKYFKLIK 180

Query: 181 PKVLKRPTDNSDDL---SAAAVDSPVREKHENGLPAGIRLVCKHLGKSKSASAAGGL--A 240
           PKVL++PTD   DL   SAAAVDSPVREKHENGLPAGIRLVC+HLGKSKSASAAGGL  A
Sbjct: 181 PKVLRKPTDTGSDLSSSSAAAVDSPVREKHENGLPAGIRLVCRHLGKSKSASAAGGLAAA 240

Query: 241 VAPPLNRRDDSLLQQHDGIQSAILHCKRSFNASTDKSV-LFSRPETAASQERSRSLSCKD 300
           V PP NRRDDSLLQQHDGIQSAILHCKRSFNAS++K + L SR ET ASQER RSLSCKD
Sbjct: 241 VVPPPNRRDDSLLQQHDGIQSAILHCKRSFNASSEKPLSLSSRSETTASQERPRSLSCKD 300

[Close]

BLAST of Tan0022699 vs. TAIR 10
Match: AT5G52870.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64080.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 115.5 bits (288), Expect = 7.1e-26
Identity = 98/269 (36.43%), Postives = 139/269 (51.67%), Query Frame = 0


Query: 29  EEEDSFFDLELLSVSAGDIQSPEQIPQIPTKLGDHINLCSDSISKRKILP-IEPTSKP-Q 88
           E EDSFF+L+   +S  D ++P++  ++ T         S   +K K+LP ++ +SKP Q
Sbjct: 45  EGEDSFFELK---ISLSDFKTPKEKQRLETTTTTTTYSVS---NKSKVLPFVDISSKPQQ 104

Query: 89  SPIQLLKSAPKFPVFIFKKQRSMAKNRAPAGAEQDESRVLNSKLSRDNST-----RRIGG 148
           SP  LLKS  KF  F FKK  S          E++    LN +   D+ T     R+   
Sbjct: 105 SPTTLLKSGQKFRAFSFKK--SEKSTTTEKKKEENNRTSLNVRFRVDDETTTTSFRKTAS 164

Query: 149 IFPSDPRGAPTTTTATARRFSKEAVQKYLRLIKPKVLKRPTDNSDDLSAAAVDSP-VREK 208
           I     R   T  T     F     +++  LIKP   K     S     +   SP  REK
Sbjct: 165 I----ARSQQTDDT----MFDDSVSKRFFSLIKPLYTKSTKKQSSSTITSPTSSPATREK 224

Query: 209 HENGLPAGIRLVCKHLGKSKSASAA-GGLAVAPPLNRRDDSLLQQHDGIQSAILHCKRSF 268
             + +P+G+R V + LGKS+SASAA GG++   P NR D+SL  Q DGIQSAILHCK+SF
Sbjct: 225 QRSNIPSGMRSVRRQLGKSRSASAAIGGMS---PANRIDESLQVQQDGIQSAILHCKKSF 284

Query: 269 NASTDKSVLFSRPETAASQERSRSLSCKD 289
           + S + S+L      ++SQE+  + S +D
Sbjct: 285 HGSRESSLLSRSTSESSSQEKLSTSSSED 294

[Close]

BLAST of Tan0022699 vs. TAIR 10
Match: AT1G64080.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G52870.1); Has 1546 Blast hits to 738 proteins in 176 species: Archae - 0; Bacteria - 143; Metazoa - 278; Fungi - 131; Plants - 95; Viruses - 4; Other Eukaryotes - 895 (source: NCBI BLink). )

HSP 1 Score: 81.6 bits (200), Expect = 1.1e-15
Identity = 67/217 (30.88%), Postives = 101/217 (46.54%), Query Frame = 0


Query: 77  LPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNRAPAGAEQDESRVLNSKLSRDNST 136
           L + P S+ +S + +     + P+     + + +KN + + +    S   +S L + N  
Sbjct: 198 LSLSPESQQKSTVTVNLKVEEVPIISLFSRENSSKNSSSSSSSSSSS---SSSLKKQNGN 257

Query: 137 RRIGGIFPSDPRGAPTTTTATARRFSKEAVQKYLRLIKP---KVLKR-----------PT 196
             +                +  +RF    +QKYL+ +KP   +V +R             
Sbjct: 258 ESV---------------VSDEKRF--VMMQKYLKKVKPLYIRVSRRYGEKLRHSGQLTA 317

Query: 197 DNSDDLSAAAVDSPVREKHEN------GLPAGIRLVCKHLGKSKSASA--------AGGL 256
            +S   +A   DSPV  K  N       +PAG+++V KHLGKS+S+S+           +
Sbjct: 318 PSSARSTAEKADSPVATKTNNKPGNNINIPAGLKVVRKHLGKSRSSSSTATTPSSTTATV 377

Query: 257 AVAPPLNRRDDSLLQQHDGIQSAILHCKRSFNASTDK 266
                  RRDDSLLQQ D IQSAILHCKRSFN+S DK
Sbjct: 378 TTQSESRRRDDSLLQQQDSIQSAILHCKRSFNSSRDK 394

The following BLAST results are available for this feature:

BLAST of Tan0022699 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FLX4	1.0e-24	36.43	Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 ...	[more]
Q9SH58	1.6e-14	30.88	Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Tan0022699 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6605748.1	2.0e-131	86.29	putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma...	[more]
XP_022996006.1	1.9e-129	85.28	probable membrane-associated kinase regulator 5 [Cucurbita maxima]	[more]
XP_023534292.1	3.2e-129	84.95	probable membrane-associated kinase regulator 5 [Cucurbita pepo subsp. pepo]	[more]
XP_022958606.1	3.6e-128	85.28	probable membrane-associated kinase regulator 5 [Cucurbita moschata]	[more]
XP_038906856.1	1.8e-124	81.97	probable membrane-associated kinase regulator 5 [Benincasa hispida]	[more]

BLAST of Tan0022699 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1K3H3	9.2e-130	85.28	probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1H2I5	1.7e-128	85.28	probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A1S3AUW4	2.7e-121	79.22	probable membrane-associated kinase regulator 5 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A0A0KK03	7.8e-121	80.26	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139900 PE=4 SV=1	[more]
A0A5D3C3T3	3.9e-120	78.46	Putative membrane-associated kinase regulator 5 OS=Cucumis melo var. makuwa OX=1...	[more]

BLAST of Tan0022699 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52870.1	7.1e-26	36.43	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G64080.1	1.1e-15	30.88	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 12..32
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 111..156
None	No IPR available	PANTHER	PTHR33929:SF4	MEMBRANE-ASSOCIATED KINASE REGULATOR 5-RELATED	coord: 1..284
IPR039619	Membrane-associated kinase regulator 2/5	PANTHER	PTHR33929	MEMBRANE-ASSOCIATED KINASE REGULATOR 2-RELATED	coord: 1..284

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0022699.1	Tan0022699.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0016301	kinase activity