Tan0022657 (gene) Snake gourd v1

Overview


Name	Tan0022657
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	gibberellin 20-oxidase-like protein
Location	LG01: 3883808 .. 3886631 (-)
RNA-Seq Expression	Tan0022657
Synteny	Tan0022657

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCAGAATTACCGATTCAGATCTGTGATGAACTTCCGGTGATCGACATGGATTATTCCGAGCATCTGAGTGAATCGAGTCGATCGAGTCTGTACAGAGCGTTTAGTGAATGGGGGATGTTCTACGTCAGAAACCATGGTGTTTCGACGGAGTTTTACCGGAAACTGAGAGCCGTCGCCGATGAACTGTTGCAGTTGCCGGAAGAGACGAAAGGGGAGAAATTGAAAGTAGGAATTTCGTGGTACACTCCGAGATTTAAGCTGTCGCCTTACTTTGAGACCTTCAAGTTTTTTGGACCAAATTTCTCTGCCTCCGCCTCCGAGCAAGGCTTCACGGAAGCGCTCTTCGGCCCCCGCGCCACTGAACTCAGGTCAACTTTCCGCCACTTAACTCACTTTTTTTTTATCTTGTGCGTGGAAGATGACGACCAAAGGATCATCTGTGGTAAAATTTTAAAAAAAATTGTGGAGGGAGAAACTGAAAAAGGAAAAAAAAAATTGTGATTCATTTGACTCAAAACCAACTAACCTAACCTAAGTTTATTAAAAGTACAACCAACCACTATGCTAGAACCAATTTTTATTAAAATTTTTTAAGTGGTTTTAATTTAAAAGTTATAATAGTTTAATATAATTATGAAAATTCTCATCAAAATGAAAATGAAGTGTTCTCCCCTCACCCATGACCTCTTAGTCATGAGTCATACTTGATACCAGCTAAATTATGTTCTTGTTGGCACGCAGTTTATGAATATTTGATTTTTAATGAAGTTATCAATCATATATGTATTAATCTTATTATATTTTAATGATTAATATTCTTGATGATAAGGACTAAATGATTAAATTTTTAAAGTATAAAAAGACTAAATTATTTTATATTAAAGTTCATGAACTAAATTGGTACCAGATTAAAAGGACAGACTTAATTATTATAAATCAAAGTTGGACTAAGTTGATACTTCAAAGGAAATTTATGTACCAAAAGTGTTTTTTAACCTAAAAATATAATTTATGTACCAAAAGTGTTTTTTAACATAAAAATAATTTCCATTTAAGAAAAAAATACAATGGGAATATGTTCTCAAAACCTTGTAGGGGAAGGGTTGACATAGATTAATTATTTTTTGAATAAATTAACTGCAAACTGACATGAACTACTAATTTTAAGATTTATTGAAAACATAAAGACTAAATTTGGATATTTGAAAGTATAGAGATAAAATCAAATAGGTCTTAAAGTACCAGGACTAGCTAAAATGATATTTTAACTAAAGATATATTAATTGAGCTTTCAAGTTTGTGTTTATTTGCTCTCTAAACTTTTAATTTTGTGTCTAATACGTAATTAAACTTTTAAAATTATCTACTTGATTCTTAAACTTTTAATTTTATGCATGTTGTTGCCAAATTTCGGACAGGAGAATACACATGACCTCCACGTGACAACGACGGTAAATTTGTAATTCTTAAGGGAAAAACGCAGCCTGCAAGACAAGGAAAACCTGGACACCGGTGTGGTGTAGGTCACACAAGCTCCGATGCCTAAGTTAGTGAGAGTCTCAGAGAGAATTATCAGGAAGTTTTTGTATAATGGTCACCAGAATTAACTTACCCTTCTCTCCTCTACTGGGAGAGATTAAATAGTTTCCCCTGGCGTTCTTCCTTGGCCTCTTGGGTAAAGGAGAGATTGTGGGGAAGTTGAATATGGGCTTTCGGTCCTTTTCGAGTCACCGTGAGGCCTGAAGATCAAACTTGTAGCTGGTTGGCCTCGACCTTGGCCGAAGCTGACCACTGGTTTGGGCCAAAACCTAAACCCAAGTCCATGGTTTGTGTCATAGGGCTTAGATAGAGCTCAAACGGAGGAGCTTTATCCTGGTCGGCTTCGACCCATGCCTGTTTTGACCTCATATTGGGCTTCTTATTCAGTCTGACTCTATGCCTTGGTCATTTGACCTGCCCATTCTCGTTGGTCCAATTTAACCAATAACAATGCCTAAGAATGATTATACTAGACAAAAAGATCAATCGAGCTATTAATTTCTTTAAAATTCAAACATATCAAACATCTATTTGACAGAAGATGGAAAATTAAGGACTCATTGTGGCAATTATTTTAACTTTTAAGATTGGTTATTTAATCTACAAAAGGTTTTCTTCATTTTATGATTGTTTTATTTTCTTCTTTCTTTAGAAACTAAAAAACTCTTTTGAAATTGAACTTGTTTGCAGCAATCTACTAGAGGAATATGGAAGGAAAATGACAGAGTTATCAAAAAGAATAGTAAAACTTTTACTAAAGACCTTGGGAGATAATTTTGAGAACAAATTCTATGATCCTGAGTTTAGTAACTGCAGTGGCTACTTGAGGATAAATAGATACTCTCCCCCAGACTTCGACGAAGAAGTTGAAGGATTCGGGAAGCACACCGACATAAGCTGCGTGACGATTCTATTCCAAGACGAGGTCGGGGGGCTTCAAATGGCGTCGAAACGAGGAGAGTGGAAGGATGTAAGGCCTGTTGAGGATGCTCTTGTGGTGAACATTGGAGATTTTATGCAAGCTTGGAGTAATGAGAGACTAAGATCAGCAGAACATAGAGTTGTTTTGAGGCAAAATGTGAAGAGATTCTCTTTGGCTTTCTTTTTGACATATGAAGATGATGATAAAGAAATATTTGCACCAAGTGAGGTTGTTGGAGAAGGAAATTCAAGGCTTTATAAGCCCTTTTCAATTAAAGAATATAGGGCGTTTAGGGAAACTAACTATGGTAATATTATTGGAGCCACATTGAAAGATTTTGCTGATCAAATGTTAGGATGA

mRNA sequence

ATGTCAGAATTACCGATTCAGATCTGTGATGAACTTCCGGTGATCGACATGGATTATTCCGAGCATCTGAGTGAATCGAGTCGATCGAGTCTGTACAGAGCGTTTAGTGAATGGGGGATGTTCTACGTCAGAAACCATGGTGTTTCGACGGAGTTTTACCGGAAACTGAGAGCCGTCGCCGATGAACTGTTGCAGTTGCCGGAAGAGACGAAAGGGGAGAAATTGAAAGTAGGAATTTCGTGGTACACTCCGAGATTTAAGCTGTCGCCTTACTTTGAGACCTTCAAGTTTTTTGGACCAAATTTCTCTGCCTCCGCCTCCGAGCAAGGCTTCACGGAAGCGCTCTTCGGCCCCCGCGCCACTGAACTCAGCAATCTACTAGAGGAATATGGAAGGAAAATGACAGAGTTATCAAAAAGAATAGTAAAACTTTTACTAAAGACCTTGGGAGATAATTTTGAGAACAAATTCTATGATCCTGAGTTTAGTAACTGCAGTGGCTACTTGAGGATAAATAGATACTCTCCCCCAGACTTCGACGAAGAAGTTGAAGGATTCGGGAAGCACACCGACATAAGCTGCGTGACGATTCTATTCCAAGACGAGGTCGGGGGGCTTCAAATGGCGTCGAAACGAGGAGAGTGGAAGGATGTAAGGCCTGTTGAGGATGCTCTTGTGGTGAACATTGGAGATTTTATGCAAGCTTGGAGTAATGAGAGACTAAGATCAGCAGAACATAGAGTTGTTTTGAGGCAAAATGTGAAGAGATTCTCTTTGGCTTTCTTTTTGACATATGAAGATGATGATAAAGAAATATTTGCACCAAGTGAGGTTGTTGGAGAAGGAAATTCAAGGCTTTATAAGCCCTTTTCAATTAAAGAATATAGGGCGTTTAGGGAAACTAACTATGGTAATATTATTGGAGCCACATTGAAAGATTTTGCTGATCAAATGTTAGGATGA

Coding sequence (CDS)

ATGTCAGAATTACCGATTCAGATCTGTGATGAACTTCCGGTGATCGACATGGATTATTCCGAGCATCTGAGTGAATCGAGTCGATCGAGTCTGTACAGAGCGTTTAGTGAATGGGGGATGTTCTACGTCAGAAACCATGGTGTTTCGACGGAGTTTTACCGGAAACTGAGAGCCGTCGCCGATGAACTGTTGCAGTTGCCGGAAGAGACGAAAGGGGAGAAATTGAAAGTAGGAATTTCGTGGTACACTCCGAGATTTAAGCTGTCGCCTTACTTTGAGACCTTCAAGTTTTTTGGACCAAATTTCTCTGCCTCCGCCTCCGAGCAAGGCTTCACGGAAGCGCTCTTCGGCCCCCGCGCCACTGAACTCAGCAATCTACTAGAGGAATATGGAAGGAAAATGACAGAGTTATCAAAAAGAATAGTAAAACTTTTACTAAAGACCTTGGGAGATAATTTTGAGAACAAATTCTATGATCCTGAGTTTAGTAACTGCAGTGGCTACTTGAGGATAAATAGATACTCTCCCCCAGACTTCGACGAAGAAGTTGAAGGATTCGGGAAGCACACCGACATAAGCTGCGTGACGATTCTATTCCAAGACGAGGTCGGGGGGCTTCAAATGGCGTCGAAACGAGGAGAGTGGAAGGATGTAAGGCCTGTTGAGGATGCTCTTGTGGTGAACATTGGAGATTTTATGCAAGCTTGGAGTAATGAGAGACTAAGATCAGCAGAACATAGAGTTGTTTTGAGGCAAAATGTGAAGAGATTCTCTTTGGCTTTCTTTTTGACATATGAAGATGATGATAAAGAAATATTTGCACCAAGTGAGGTTGTTGGAGAAGGAAATTCAAGGCTTTATAAGCCCTTTTCAATTAAAGAATATAGGGCGTTTAGGGAAACTAACTATGGTAATATTATTGGAGCCACATTGAAAGATTTTGCTGATCAAATGTTAGGATGA

Protein sequence

MSELPIQICDELPVIDMDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVADELLQLPEETKGEKLKVGISWYTPRFKLSPYFETFKFFGPNFSASASEQGFTEALFGPRATELSNLLEEYGRKMTELSKRIVKLLLKTLGDNFENKFYDPEFSNCSGYLRINRYSPPDFDEEVEGFGKHTDISCVTILFQDEVGGLQMASKRGEWKDVRPVEDALVVNIGDFMQAWSNERLRSAEHRVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGEGNSRLYKPFSIKEYRAFRETNYGNIIGATLKDFADQMLG

Homology

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BLAST of Tan0022657 vs. ExPASy Swiss-Prot
Match: F4KBY0 (Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310 PE=2 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 2.6e-71
Identity = 151/326 (46.32%), Postives = 212/326 (65.03%), Query Frame = 0


Query: 11  ELPVIDMDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVADELLQLPEET 70
           ELPV D+  S+ LSESS +SL  A  EWG FYV NHGVS + Y+KLR  +  + +L +E 
Sbjct: 4   ELPVFDI--SKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDE- 63

Query: 71  KGEKLKVGISWYTPRFKLSPYFETFKFFGPNFSASASEQGFTEALFGPRAT--ELSNLLE 130
             EK+K+G S YTPRF  SP+FE+ +  GP+F ASA     +   F  +AT  E S L++
Sbjct: 64  --EKMKMGASNYTPRFIASPFFESLRVSGPDFYASAKS---SVDAFSDQATDEEFSGLMK 123

Query: 131 EYGRKMTELSKRIVKLLLKTLGDNFENKFYDPEFSNCSGYLRINRYSPPDFDEE------ 190
           EYG KMT+L ++I+K +L + GD+  +K+Y+ EF NC GY RIN Y+ P   E+      
Sbjct: 124 EYGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGD 183

Query: 191 ----VEGFGKHTDISCVTILFQDEVGGLQMASKRG-EWKDVRPVEDALVVNIGDFMQAWS 250
               +EG G HTD+SC+TI+ QD++GGLQ+ ++ G    D+ P ++ALVVN+GD + AW+
Sbjct: 184 EQDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLLHAWT 243

Query: 251 NERLRSAEHRVVLRQN---VKRFSLAFFLTYEDDDKEIFAPSEVVG--EGNSRLYKPFSI 310
           N RLRS++HRV+L++      RFSLAFF  + DD K +FAP EVVG  EG  R+++ F  
Sbjct: 244 NGRLRSSQHRVILKRRGFVGNRFSLAFFWCF-DDGKVVFAPDEVVGGCEG-MRVFRSFKC 303

Query: 311 KEYRAFRETNYG---NIIGATLKDFA 316
            +Y  FRE+N       +G T++DFA
Sbjct: 304 GDYLRFRESNEKGKFEKVGDTVEDFA 319

[Close]

BLAST of Tan0022657 vs. ExPASy Swiss-Prot
Match: O49561 (Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 SV=2)

HSP 1 Score: 147.9 bits (372), Expect = 2.0e-34
Identity = 98/301 (32.56%), Postives = 147/301 (48.84%), Query Frame = 0


Query: 11  ELPVID----MDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVADELLQL 70
           ELPVID    +D +E   E  + ++ RA  EWG F V NHG+S +   K+R     + + 
Sbjct: 41  ELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFRE 100

Query: 71  P--EETKGEKLKVG-ISWYTPRFKLSPYFETFKFFGPNFSASASEQGFTEALFGPRA--- 130
           P  +++K EK   G   W TP                  + S  +  ++EA   P     
Sbjct: 101 PFDKKSKSEKFSAGSYRWGTPS-----------------ATSIRQLSWSEAFHVPMTDIS 160

Query: 131 -----TELSNLLEEYGRKMTELSKRIVKLLLKTLGDNFENKFYDPEFSNCSGYLRINRYS 190
                T LS+ +E++  +   L+  + ++L +  G N  + F+       + YLR+NRY 
Sbjct: 161 DNKDFTTLSSTMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYP 220

Query: 191 PPDFDEEVEGFGKHTDISCVTILFQDEVGGLQMASKRGEWKDVRPVEDALVVNIGDFMQA 250
           P     EV G   HTD   +TIL+QD+VGGLQ+  K   W  V+P   AL++NIGD  QA
Sbjct: 221 PCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLI-KDNRWIAVKPNPKALIINIGDLFQA 280

Query: 251 WSNERLRSAEHRVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGEGNSRLYKPFSIKEY 297
           WSN   +S EHRV+    V+RFS A+F+    D       + +    +   Y+ FS +E+
Sbjct: 281 WSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYD-------AVIECSSDRPAYRNFSFREF 314

[Close]

BLAST of Tan0022657 vs. ExPASy Swiss-Prot
Match: Q8LGZ9 (Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX5 PE=1 SV=1)

HSP 1 Score: 136.0 bits (341), Expect = 7.7e-31
Identity = 98/311 (31.51%), Postives = 143/311 (45.98%), Query Frame = 0


Query: 1   MSELPIQICDELPVIDMDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVA 60
           M  + +  C ELPVID+       +  R+ + RA SEWG F V NHGV     R+L    
Sbjct: 32  MGAIDVDEC-ELPVIDLAGLMEAEQVCRADMVRAASEWGFFQVTNHGVPQALLRELHDAQ 91

Query: 61  DELLQLPEETKGEKLKVGIS-----WYTPRFKLSPYFETFKFFGPNFSASASEQGFTEAL 120
             + + P + K  +  +G S     W TP  K                    +  ++EA 
Sbjct: 92  VAVFRRPFQEKVTERLLGFSPESYRWGTPTAK-----------------CLEQLSWSEAY 151

Query: 121 FGPRATELSN-------LLEEYGRKMTELSKRIVKLLLKTL-GDNFENKFYDPEFSNCSG 180
             P  T   +       ++EE  R M EL++++ ++L++ L G              C  
Sbjct: 152 HIPMTTPRPSTSIRARAVIEEVSRAMYELAQKLAEILMRGLPGAGEGETMVTTREETC-- 211

Query: 181 YLRINRYSPPDFDEEVEGFGKHTDISCVTILF--QDEVGGLQMASKRGEWKDVRPVEDAL 240
           +LR+NRY P        G   HTD   +TI+   QD VGGLQ+  K G W  V+P    L
Sbjct: 212 FLRLNRYPPCAMAMGGFGLCPHTDSDLLTIVHQQQDTVGGLQLL-KGGRWVAVKPSPSTL 271

Query: 241 VVNIGDFMQAWSNERLRSAEHRVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGEGNSR 297
           +VN+GD +QAWSN+  +S EHRV+    ++RFS+AFFL        +  PS      +  
Sbjct: 272 IVNVGDLLQAWSNDVYKSVEHRVMANATLERFSMAFFLC--PSYHTLIIPSSSHVHDDDA 319

[Close]

BLAST of Tan0022657 vs. ExPASy Swiss-Prot
Match: Q7XP65 (Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX6 PE=1 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 3.8e-30
Identity = 96/299 (32.11%), Postives = 137/299 (45.82%), Query Frame = 0


Query: 11  ELPVIDMDYSEHLSESSR----SSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVADELLQL 70
           +LP++D++         R     ++ RA SEWG F + NHGV  E   ++R     L +L
Sbjct: 49  DLPMVDLERLTRGGAGERKACAGAMARAASEWGFFQLTNHGVGRELMEEMRREQARLFRL 108

Query: 71  PEETKGEK--LKVGISWYTP-RFKLSPYFETFKFFGPNFSASASEQGFTEALFGPRATEL 130
           P ETK +   L     W  P    L     +  F  P  S S ++  F +       T L
Sbjct: 109 PFETKEKAGLLNGSYRWGNPTATSLRHLSWSEAFHVPLASISGADCDFGD------LTSL 168

Query: 131 SNLLEEYGRKMTELSKRIVKLLLKTL-----GDNFENKFYDPEFSNCSGYLRINRYSPPD 190
             +++E    M+ ++  +   L + L     G      ++       + +LR+NRY    
Sbjct: 169 RGVMQEVAEAMSRVANTVAAALAEELTGRGGGGASAAPWFPAGCDETTCFLRLNRYPACP 228

Query: 191 FDEEVEGFGKHTDISCVTILFQDEVGGLQMASKRGEWKDVRPVEDALVVNIGDFMQAWSN 250
           F  +  G   HTD   +T+L QD+VGGL +  K   W  VRP  DALVVNIGD  QAWSN
Sbjct: 229 FAADTFGLVPHTDSDFLTVLCQDQVGGLHL-MKDSRWVAVRPRPDALVVNIGDLFQAWSN 288

Query: 251 ERLRSAEHRVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGE-GNSRLYKPFSIKEYR 297
            R +S EH+VV      R S+A+FL    D         +VG  G    Y+ F+  EYR
Sbjct: 289 NRYKSVEHKVVANAKTDRLSVAYFLCPSYD--------SLVGTCGEPSPYRAFTFGEYR 332

[Close]

BLAST of Tan0022657 vs. ExPASy Swiss-Prot
Match: P0C5H5 (Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica OX=39946 GN=20ox2 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 9.4e-29
Identity = 91/272 (33.46%), Postives = 135/272 (49.63%), Query Frame = 0


Query: 11  ELPVIDMDYSEHLSESSRSSLYRAFSE-------WGMFYVRNHGVSTEFYRKLRAVADEL 70
           ++PV+D+     L +     L RA ++        G F V  HGV     R     A + 
Sbjct: 62  DMPVVDVGV---LRDGDAEGLRRAAAQVAAACATHGFFQVSEHGVDAALARAALDGASDF 121

Query: 71  LQLP--EETKGEKLKVGISWYT----PRFKLS-PYFETFKFFGPNFSASASEQGFTEALF 130
            +LP  E+ +  ++   +S YT     RF    P+ ET  F   + +A+     +  +  
Sbjct: 122 FRLPLAEKRRARRVPGTVSGYTSAHADRFASKLPWKETLSFGFHDRAAAPVVADYFSSTL 181

Query: 131 GPRATELSNLLEEYGRKMTELSKRIVKLLLKTLGDNFENKFYDPEFSNCSGYLRINRYSP 190
           GP    +  + ++Y  +M ELS  I++LL  +LG   E  +Y   F++ S  +R N Y P
Sbjct: 182 GPDFAPMGRVYQKYCEEMKELSLTIMELLELSLG--VERGYYREFFADSSSIMRCNYYPP 241

Query: 191 PDFDEEVEGFGKHTDISCVTILFQDEVGGLQMASKRGEWKDVRPVEDALVVNIGDFMQAW 250
               E   G G H D + +TIL QD+VGGL++    GEW+ V PV  A+V+NIGD   A 
Sbjct: 242 CPEPERTLGTGPHCDPTALTILLQDDVGGLEVLVD-GEWRPVSPVPGAMVINIGDTFMAL 301

Query: 251 SNERLRSAEHRVVLRQNVKRFSLAFFLTYEDD 269
           SN R +S  HR V+ Q  +R SLAFFL   +D
Sbjct: 302 SNGRYKSCLHRAVVNQRRERRSLAFFLCPRED 327

[Close]

BLAST of Tan0022657 vs. NCBI nr
Match: XP_008453897.1 (PREDICTED: gibberellin 2-beta-dioxygenase 8 [Cucumis melo] >TYK16936.1 gibberellin 2-beta-dioxygenase 8 [Cucumis melo var. makuwa])

HSP 1 Score: 473.4 bits (1217), Expect = 1.6e-129
Identity = 223/317 (70.35%), Postives = 270/317 (85.17%), Query Frame = 0


Query: 1   MSELPIQICDELPVIDMDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVA 60
           MS+  I++C+ELPV+DM+  EHLSESSR SLY+A +EWG FY++NHGVS E Y KLRAV 
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  DELL-QLPEETKGEKLKVGISWYTPRFKLSPYFETFKFFGPNFSASASEQGFTEALFGPR 120
           DELL  +PEE K  KLKVG+SWYTPRFKLSPY E+FKF GP FS SAS+ GFTE +FG R
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 ATELSNLLEEYGRKMTELSKRIVKLLLKTLGDNFENKFYDPEFSNCSGYLRINRYSPPDF 180
            T+  NLL+EYG  M ELS+RI+KLLLK +GD+FE+KFY+ EFSNC+GY+RINRY+P + 
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 DEEVEGFGKHTDISCVTILFQDEVGGLQMASKRG-EWKDVRPVEDALVVNIGDFMQAWSN 240
           +EE+E FGKHTDISCVTILFQDE+GG+QM SK+G EW DVRP+EDAL+VNIGDF+QAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGEGNSRLYKPFSIKEYRAF 300
           ERLRSAEHRVVL+QN KRF+L FFL + DDD+E++APSEVVGEGN R+YKPFS KEYRAF
Sbjct: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RETNYGNIIGATLKDFA 316
           RE NYG ++G  +++FA
Sbjct: 301 RENNYGKVVGVPMREFA 317

[Close]

BLAST of Tan0022657 vs. NCBI nr
Match: XP_038876311.1 (gibberellin 20-oxidase-like protein [Benincasa hispida])

HSP 1 Score: 471.1 bits (1211), Expect = 7.7e-129
Identity = 228/316 (72.15%), Postives = 268/316 (84.81%), Query Frame = 0


Query: 1   MSELPIQICDELPVIDMDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVA 60
           MSE  I++ +ELPV+D+   + LSESSRS LY+A +EWG FY+RNHGVS E Y KLRAV 
Sbjct: 1   MSEESIEMYEELPVMDIHCCDDLSESSRSCLYKACNEWGHFYIRNHGVSKELYLKLRAVT 60

Query: 61  DELLQLPEETKGEKLKVGISWYTPRFKLSPYFETFKFFGPNFSASASEQGFTEALFGPRA 120
           DEL  L EE K  KLK G+SWYTPRF LSPY E+FKF GPNFSASAS+ GFTE +FG R 
Sbjct: 61  DEL--LTEERKEGKLKEGVSWYTPRFILSPYIESFKFLGPNFSASASDLGFTEQVFGQRV 120

Query: 121 TELSNLLEEYGRKMTELSKRIVKLLLKTLGDNFENKFYDPEFSNCSGYLRINRYSPPDFD 180
           T+LSNLL+EYG  M ELS++I+KLLLK +GDN ENKFY+ EFS C+GY+RINRY+P + +
Sbjct: 121 TQLSNLLDEYGSIMIELSRKIMKLLLKIMGDNLENKFYESEFSKCNGYIRINRYAPRNSN 180

Query: 181 EEVEGFGKHTDISCVTILFQDEVGGLQMASKRG-EWKDVRPVEDALVVNIGDFMQAWSNE 240
           EE+E FGKHTDISCVTILFQDE+GGLQM SK+G EW DV+P+EDAL+VNIGDFMQAWSNE
Sbjct: 181 EEIEAFGKHTDISCVTILFQDEIGGLQMKSKQGDEWIDVKPLEDALLVNIGDFMQAWSNE 240

Query: 241 RLRSAEHRVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGEGNSRLYKPFSIKEYRAFR 300
           RLRSAEHRVVL+QNVKRFSLAFFL + DDD+EI+APSEVVGEGN+RLYKPFS KEYRAFR
Sbjct: 241 RLRSAEHRVVLKQNVKRFSLAFFLIFGDDDREIYAPSEVVGEGNTRLYKPFSTKEYRAFR 300

Query: 301 ETNYGNIIGATLKDFA 316
           E NYG ++G  L++FA
Sbjct: 301 EYNYGKVVGTPLREFA 314

[Close]

BLAST of Tan0022657 vs. NCBI nr
Match: KAA0044653.1 (gibberellin 2-beta-dioxygenase 8 [Cucumis melo var. makuwa])

HSP 1 Score: 470.3 bits (1209), Expect = 1.3e-128
Identity = 221/317 (69.72%), Postives = 269/317 (84.86%), Query Frame = 0


Query: 1   MSELPIQICDELPVIDMDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVA 60
           MS+  I++C+ELPV+DM+  EHLSESSR SLY+A +EWG FY++NHGVS E Y KLRAV 
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  DELL-QLPEETKGEKLKVGISWYTPRFKLSPYFETFKFFGPNFSASASEQGFTEALFGPR 120
           DELL  +PEE K  KLKVG+SWYTPRFKLSPY E+FKF GP FS SAS+ GFTE +FG R
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 ATELSNLLEEYGRKMTELSKRIVKLLLKTLGDNFENKFYDPEFSNCSGYLRINRYSPPDF 180
            T+  NLL+EYG  M ELS+RI+KLLLK +GD+FE+KFY+ EFSNC+GY+RINRY+P + 
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 DEEVEGFGKHTDISCVTILFQDEVGGLQMASKRG-EWKDVRPVEDALVVNIGDFMQAWSN 240
           +EE+E FGKHTDISCVTILFQDE+GG+QM SK+G EW DVRP+EDAL+VNIGDF+QAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGEGNSRLYKPFSIKEYRAF 300
           ERLRS EHRVVL+QN +RF+L FFL + DDD+E++APSEVVGEGN R+YKPFS KEYRAF
Sbjct: 241 ERLRSTEHRVVLKQNAERFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RETNYGNIIGATLKDFA 316
           RE NYG ++G  +++FA
Sbjct: 301 RENNYGKVVGVPMREFA 317

[Close]

BLAST of Tan0022657 vs. NCBI nr
Match: XP_004146911.1 (gibberellin 20-oxidase-like protein [Cucumis sativus] >KGN53154.1 hypothetical protein Csa_014948 [Cucumis sativus])

HSP 1 Score: 465.3 bits (1196), Expect = 4.2e-127
Identity = 222/317 (70.03%), Postives = 270/317 (85.17%), Query Frame = 0


Query: 1   MSELPIQICDELPVIDMDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVA 60
           MS+  I++ +EL V+DM+  EHLSESSR SLY+A +EWG FY++NHGVS E Y+KLRAV 
Sbjct: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60

Query: 61  DELL-QLPEETKGEKLKVGISWYTPRFKLSPYFETFKFFGPNFSASASEQGFTEALFGPR 120
           DELL  +PEETK  KLKVG SWYTPRF+LSPY E+FKF GPNFS  AS+ GFTE +FG R
Sbjct: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120

Query: 121 ATELSNLLEEYGRKMTELSKRIVKLLLKTLGDNFENKFYDPEFSNCSGYLRINRYSPPDF 180
            T+   LL+EYG  M ELS+RI+KLLLKT+GDN E+KFY+ EFSNC+GYLRINRY+P + 
Sbjct: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180

Query: 181 DEEVEGFGKHTDISCVTILFQDEVGGLQMASKRG-EWKDVRPVEDALVVNIGDFMQAWSN 240
           +EE+E FGKHTDISCVTI+FQDE+GG+QM  K+G EW DVRP+EDAL+VNIGDF+QAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGEGNSRLYKPFSIKEYRAF 300
           ERLRSAEHRVVL+Q+VKRFSLAFFL ++DDD+E++APSEVVGEGN+R+YKPFS KEYRA+
Sbjct: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300

Query: 301 RETNYGNIIGATLKDFA 316
           RE NY  I+G  L++FA
Sbjct: 301 RENNYRKIVGVPLREFA 317

[Close]

BLAST of Tan0022657 vs. NCBI nr
Match: XP_022141254.1 (gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia])

HSP 1 Score: 423.3 bits (1087), Expect = 1.8e-114
Identity = 219/312 (70.19%), Postives = 248/312 (79.49%), Query Frame = 0


Query: 7   QICDELPVIDMDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVADELLQL 66
           Q C ELPVID+  S+ LSES   SL  A +EWG FY+ NHGVS EFY+KL ++ADEL +L
Sbjct: 5   QTCVELPVIDL--SQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELFRL 64

Query: 67  PEETKGEKLKVGISWYTPRFKLSPYFETFKFFGPNFSASASEQGFTEALFGPRATELSNL 126
           P++T   K+KVGIS Y PRF  SP+FE+FK  GP+FSASA  Q  TEAL G   T+ SNL
Sbjct: 65  PQDT---KIKVGISSYVPRFTASPFFESFKLSGPDFSASA--QLLTEALLGHHNTQFSNL 124

Query: 127 LEEYGRKMTELSKRIVKLLLKTLGDNFENKFYDPEFSNCSGYLRINRYSPPDFDEEVEGF 186
           LEEYG KMT+LSKRIVKLLLK LGDNFENKFYD EF NC GYLRIN YSP D +EEVEG 
Sbjct: 125 LEEYGSKMTBLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGL 184

Query: 187 GKHTDISCVTILFQDEVGGLQMASKRGEWKDVRPVEDALVVNIGDFMQAWSNERLRSAEH 246
           GKHTDISCVTILFQD++GGLQM SK GEW D+RP E+ALVVNIGDFMQAWSNERLRSAEH
Sbjct: 185 GKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEH 244

Query: 247 RVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGEGNSRLYKPFSIKEYRAFRETNYGNI 306
           RVVLRQNVKRFS+AFF +Y DD+ EI+APSEVVGEGN RLY+ FS KEYR FR T     
Sbjct: 245 RVVLRQNVKRFSMAFFWSYVDDE-EIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGT 304

Query: 307 ---IGATLKDFA 316
              +GA +K+FA
Sbjct: 305 YEKVGANVKEFA 308

[Close]

BLAST of Tan0022657 vs. ExPASy TrEMBL
Match: A0A5D3D2E7 (Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00730 PE=3 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 7.5e-130
Identity = 223/317 (70.35%), Postives = 270/317 (85.17%), Query Frame = 0


Query: 1   MSELPIQICDELPVIDMDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVA 60
           MS+  I++C+ELPV+DM+  EHLSESSR SLY+A +EWG FY++NHGVS E Y KLRAV 
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  DELL-QLPEETKGEKLKVGISWYTPRFKLSPYFETFKFFGPNFSASASEQGFTEALFGPR 120
           DELL  +PEE K  KLKVG+SWYTPRFKLSPY E+FKF GP FS SAS+ GFTE +FG R
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 ATELSNLLEEYGRKMTELSKRIVKLLLKTLGDNFENKFYDPEFSNCSGYLRINRYSPPDF 180
            T+  NLL+EYG  M ELS+RI+KLLLK +GD+FE+KFY+ EFSNC+GY+RINRY+P + 
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 DEEVEGFGKHTDISCVTILFQDEVGGLQMASKRG-EWKDVRPVEDALVVNIGDFMQAWSN 240
           +EE+E FGKHTDISCVTILFQDE+GG+QM SK+G EW DVRP+EDAL+VNIGDF+QAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGEGNSRLYKPFSIKEYRAF 300
           ERLRSAEHRVVL+QN KRF+L FFL + DDD+E++APSEVVGEGN R+YKPFS KEYRAF
Sbjct: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RETNYGNIIGATLKDFA 316
           RE NYG ++G  +++FA
Sbjct: 301 RENNYGKVVGVPMREFA 317

[Close]

BLAST of Tan0022657 vs. ExPASy TrEMBL
Match: A0A1S3BYJ4 (gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo OX=3656 GN=LOC103494477 PE=3 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 7.5e-130
Identity = 223/317 (70.35%), Postives = 270/317 (85.17%), Query Frame = 0


Query: 1   MSELPIQICDELPVIDMDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVA 60
           MS+  I++C+ELPV+DM+  EHLSESSR SLY+A +EWG FY++NHGVS E Y KLRAV 
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  DELL-QLPEETKGEKLKVGISWYTPRFKLSPYFETFKFFGPNFSASASEQGFTEALFGPR 120
           DELL  +PEE K  KLKVG+SWYTPRFKLSPY E+FKF GP FS SAS+ GFTE +FG R
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 ATELSNLLEEYGRKMTELSKRIVKLLLKTLGDNFENKFYDPEFSNCSGYLRINRYSPPDF 180
            T+  NLL+EYG  M ELS+RI+KLLLK +GD+FE+KFY+ EFSNC+GY+RINRY+P + 
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 DEEVEGFGKHTDISCVTILFQDEVGGLQMASKRG-EWKDVRPVEDALVVNIGDFMQAWSN 240
           +EE+E FGKHTDISCVTILFQDE+GG+QM SK+G EW DVRP+EDAL+VNIGDF+QAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGEGNSRLYKPFSIKEYRAF 300
           ERLRSAEHRVVL+QN KRF+L FFL + DDD+E++APSEVVGEGN R+YKPFS KEYRAF
Sbjct: 241 ERLRSAEHRVVLKQNAKRFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RETNYGNIIGATLKDFA 316
           RE NYG ++G  +++FA
Sbjct: 301 RENNYGKVVGVPMREFA 317

[Close]

BLAST of Tan0022657 vs. ExPASy TrEMBL
Match: A0A5A7TME4 (Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold46G004080 PE=3 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 6.4e-129
Identity = 221/317 (69.72%), Postives = 269/317 (84.86%), Query Frame = 0


Query: 1   MSELPIQICDELPVIDMDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVA 60
           MS+  I++C+ELPV+DM+  EHLSESSR SLY+A +EWG FY++NHGVS E Y KLRAV 
Sbjct: 1   MSKESIEMCEELPVMDMNCCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYWKLRAVT 60

Query: 61  DELL-QLPEETKGEKLKVGISWYTPRFKLSPYFETFKFFGPNFSASASEQGFTEALFGPR 120
           DELL  +PEE K  KLKVG+SWYTPRFKLSPY E+FKF GP FS SAS+ GFTE +FG R
Sbjct: 61  DELLTAVPEEAKERKLKVGVSWYTPRFKLSPYAESFKFLGPIFSDSASDLGFTEQVFGHR 120

Query: 121 ATELSNLLEEYGRKMTELSKRIVKLLLKTLGDNFENKFYDPEFSNCSGYLRINRYSPPDF 180
            T+  NLL+EYG  M ELS+RI+KLLLK +GD+FE+KFY+ EFSNC+GY+RINRY+P + 
Sbjct: 121 VTQFRNLLDEYGSIMIELSRRIMKLLLKMMGDSFEDKFYESEFSNCNGYIRINRYAPRNS 180

Query: 181 DEEVEGFGKHTDISCVTILFQDEVGGLQMASKRG-EWKDVRPVEDALVVNIGDFMQAWSN 240
           +EE+E FGKHTDISCVTILFQDE+GG+QM SK+G EW DVRP+EDAL+VNIGDF+QAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTILFQDEIGGIQMKSKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGEGNSRLYKPFSIKEYRAF 300
           ERLRS EHRVVL+QN +RF+L FFL + DDD+E++APSEVVGEGN R+YKPFS KEYRAF
Sbjct: 241 ERLRSTEHRVVLKQNAERFTLGFFLVFGDDDRELYAPSEVVGEGNRRVYKPFSTKEYRAF 300

Query: 301 RETNYGNIIGATLKDFA 316
           RE NYG ++G  +++FA
Sbjct: 301 RENNYGKVVGVPMREFA 317

[Close]

BLAST of Tan0022657 vs. ExPASy TrEMBL
Match: A0A0A0KW97 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G022900 PE=3 SV=1)

HSP 1 Score: 465.3 bits (1196), Expect = 2.0e-127
Identity = 222/317 (70.03%), Postives = 270/317 (85.17%), Query Frame = 0


Query: 1   MSELPIQICDELPVIDMDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVA 60
           MS+  I++ +EL V+DM+  EHLSESSR SLY+A +EWG FY++NHGVS E Y+KLRAV 
Sbjct: 1   MSKESIEMHEELLVMDMNSCEHLSESSRLSLYKACNEWGHFYIKNHGVSKELYQKLRAVT 60

Query: 61  DELL-QLPEETKGEKLKVGISWYTPRFKLSPYFETFKFFGPNFSASASEQGFTEALFGPR 120
           DELL  +PEETK  KLKVG SWYTPRF+LSPY E+FKF GPNFS  AS+ GFTE +FG R
Sbjct: 61  DELLTAVPEETKEGKLKVGASWYTPRFRLSPYIESFKFLGPNFSDYASDLGFTEQVFGQR 120

Query: 121 ATELSNLLEEYGRKMTELSKRIVKLLLKTLGDNFENKFYDPEFSNCSGYLRINRYSPPDF 180
            T+   LL+EYG  M ELS+RI+KLLLKT+GDN E+KFY+ EFSNC+GYLRINRY+P + 
Sbjct: 121 VTQFRKLLDEYGSIMMELSRRIMKLLLKTMGDNLEDKFYESEFSNCNGYLRINRYAPRNS 180

Query: 181 DEEVEGFGKHTDISCVTILFQDEVGGLQMASKRG-EWKDVRPVEDALVVNIGDFMQAWSN 240
           +EE+E FGKHTDISCVTI+FQDE+GG+QM  K+G EW DVRP+EDAL+VNIGDF+QAWSN
Sbjct: 181 NEEIEAFGKHTDISCVTIIFQDEIGGIQMKWKQGDEWVDVRPLEDALLVNIGDFLQAWSN 240

Query: 241 ERLRSAEHRVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGEGNSRLYKPFSIKEYRAF 300
           ERLRSAEHRVVL+Q+VKRFSLAFFL ++DDD+E++APSEVVGEGN+R+YKPFS KEYRA+
Sbjct: 241 ERLRSAEHRVVLKQDVKRFSLAFFLIFKDDDRELYAPSEVVGEGNTRIYKPFSTKEYRAY 300

Query: 301 RETNYGNIIGATLKDFA 316
           RE NY  I+G  L++FA
Sbjct: 301 RENNYRKIVGVPLREFA 317

[Close]

BLAST of Tan0022657 vs. ExPASy TrEMBL
Match: A0A6J1CI26 (gibberellin 20-oxidase-like protein isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011697 PE=3 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 6.8e-115
Identity = 219/312 (70.19%), Postives = 248/312 (79.49%), Query Frame = 0


Query: 7   QICDELPVIDMDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVADELLQL 66
           Q C ELPVID+  S+ LSES   SL  A +EWG FY+ NHGVS EFY+KL ++ADEL +L
Sbjct: 5   QTCVELPVIDL--SQPLSESILCSLCAACNEWGFFYITNHGVSDEFYQKLISIADELFRL 64

Query: 67  PEETKGEKLKVGISWYTPRFKLSPYFETFKFFGPNFSASASEQGFTEALFGPRATELSNL 126
           P++T   K+KVGIS Y PRF  SP+FE+FK  GP+FSASA  Q  TEAL G   T+ SNL
Sbjct: 65  PQDT---KIKVGISSYVPRFTASPFFESFKLSGPDFSASA--QLLTEALLGHHNTQFSNL 124

Query: 127 LEEYGRKMTELSKRIVKLLLKTLGDNFENKFYDPEFSNCSGYLRINRYSPPDFDEEVEGF 186
           LEEYG KMT+LSKRIVKLLLK LGDNFENKFYD EF NC GYLRIN YSP D +EEVEG 
Sbjct: 125 LEEYGSKMTDLSKRIVKLLLKVLGDNFENKFYDYEFKNCDGYLRINNYSPRDSNEEVEGL 184

Query: 187 GKHTDISCVTILFQDEVGGLQMASKRGEWKDVRPVEDALVVNIGDFMQAWSNERLRSAEH 246
           GKHTDISCVTILFQD++GGLQM SK GEW D+RP E+ALVVNIGDFMQAWSNERLRSAEH
Sbjct: 185 GKHTDISCVTILFQDKLGGLQMRSKGGEWMDIRPCENALVVNIGDFMQAWSNERLRSAEH 244

Query: 247 RVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGEGNSRLYKPFSIKEYRAFRETNYGNI 306
           RVVLRQNVKRFS+AFF +Y DD+ EI+APSEVVGEGN RLY+ FS KEYR FR T     
Sbjct: 245 RVVLRQNVKRFSMAFFWSYVDDE-EIYAPSEVVGEGNVRLYRTFSTKEYRMFRATGARGT 304

Query: 307 ---IGATLKDFA 316
              +GA +K+FA
Sbjct: 305 YEKVGANVKEFA 308

[Close]

BLAST of Tan0022657 vs. TAIR 10
Match: AT4G23340.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 297.0 bits (759), Expect = 1.9e-80
Identity = 165/324 (50.93%), Postives = 212/324 (65.43%), Query Frame = 0


Query: 1   MSELPIQICDELPVIDMDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVA 60
           MSEL   +  +LPV+D+  ++ +  S  SSL  A  EWG FYV NHG+S E + K+ +++
Sbjct: 1   MSELHSSL--QLPVLDL--TQPIESSILSSLSEACKEWGFFYVTNHGISKEMFSKICSLS 60

Query: 61  DELLQLPEETKGEKLKVGISWYTPRFKLSPYFETFKFFGPNFSASASEQGFTEALF-GPR 120
            ++ + P E+   KLK+G   YTPR+  SPYFE+    GP+FS SA  +   + LF    
Sbjct: 61  RDVFKAPLES---KLKLGPISYTPRYIASPYFESLVVSGPDFSDSA--KASADVLFQDHH 120

Query: 121 ATELSNLLEEYGRKMTELSKRIVK-LLLKTLGDNFENKFYDPEFSNCSGYLRINRYSPP- 180
             EL   ++EYG KM ELSKR++K LL+ TLGD    + Y  +FSNC GYLR+  Y+PP 
Sbjct: 121 KPELRETMQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPH 180

Query: 181 ---DFDEEVEGFGKHTDISCVTILFQDEVGGLQMASKRGEWKDVRPVEDALVVNIGDFMQ 240
                +E VEG G HTD+SC+TI++QD VGGLQM SK G+W D+ P  D LVVNIGD MQ
Sbjct: 181 DVEKQEELVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCNDFLVVNIGDLMQ 240

Query: 241 AWSNERLRSAEHRVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGEGNSRLYKPFSIKE 300
           AWSN RLRS+EHRVVLR+ V R SLAFFL +E D+K I AP E+VGEG  R YK F   E
Sbjct: 241 AWSNGRLRSSEHRVVLRKLVNRVSLAFFLCFE-DEKVILAPQEIVGEGKQRSYKSFKCSE 300

Query: 301 YRAFRETNYG---NIIGATLKDFA 316
           Y  FR++N       IG T+KDFA
Sbjct: 301 YLKFRQSNEEGKFEKIGFTVKDFA 314

[Close]

BLAST of Tan0022657 vs. TAIR 10
Match: AT5G51310.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 270.4 bits (690), Expect = 1.9e-72
Identity = 151/326 (46.32%), Postives = 212/326 (65.03%), Query Frame = 0


Query: 11  ELPVIDMDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVADELLQLPEET 70
           ELPV D+  S+ LSESS +SL  A  EWG FYV NHGVS + Y+KLR  +  + +L +E 
Sbjct: 4   ELPVFDI--SKPLSESSLTSLQDACKEWGFFYVTNHGVSRDMYKKLRRFSTGVFELEDE- 63

Query: 71  KGEKLKVGISWYTPRFKLSPYFETFKFFGPNFSASASEQGFTEALFGPRAT--ELSNLLE 130
             EK+K+G S YTPRF  SP+FE+ +  GP+F ASA     +   F  +AT  E S L++
Sbjct: 64  --EKMKMGASNYTPRFIASPFFESLRVSGPDFYASAKS---SVDAFSDQATDEEFSGLMK 123

Query: 131 EYGRKMTELSKRIVKLLLKTLGDNFENKFYDPEFSNCSGYLRINRYSPPDFDEE------ 190
           EYG KMT+L ++I+K +L + GD+  +K+Y+ EF NC GY RIN Y+ P   E+      
Sbjct: 124 EYGEKMTKLCEKIMKAILSSFGDDLHHKYYESEFGNCHGYFRINNYTIPSDQEDDHHNGD 183

Query: 191 ----VEGFGKHTDISCVTILFQDEVGGLQMASKRG-EWKDVRPVEDALVVNIGDFMQAWS 250
               +EG G HTD+SC+TI+ QD++GGLQ+ ++ G    D+ P ++ALVVN+GD + AW+
Sbjct: 184 EQDLIEGLGMHTDMSCITIVDQDDIGGLQVRTRDGIGLMDINPKDEALVVNVGDLLHAWT 243

Query: 251 NERLRSAEHRVVLRQN---VKRFSLAFFLTYEDDDKEIFAPSEVVG--EGNSRLYKPFSI 310
           N RLRS++HRV+L++      RFSLAFF  + DD K +FAP EVVG  EG  R+++ F  
Sbjct: 244 NGRLRSSQHRVILKRRGFVGNRFSLAFFWCF-DDGKVVFAPDEVVGGCEG-MRVFRSFKC 303

Query: 311 KEYRAFRETNYG---NIIGATLKDFA 316
            +Y  FRE+N       +G T++DFA
Sbjct: 304 GDYLRFRESNEKGKFEKVGDTVEDFA 319

[Close]

BLAST of Tan0022657 vs. TAIR 10
Match: AT4G23340.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 226.9 bits (577), Expect = 2.4e-59
Identity = 115/197 (58.38%), Postives = 139/197 (70.56%), Query Frame = 0


Query: 127 LEEYGRKMTELSKRIVK-LLLKTLGDNFENKFYDPEFSNCSGYLRINRYSPP----DFDE 186
           ++EYG KM ELSKR++K LL+ TLGD    + Y  +FSNC GYLR+  Y+PP      +E
Sbjct: 1   MQEYGAKMAELSKRLIKILLMMTLGDETGKRLYQTDFSNCHGYLRLVNYTPPHDVEKQEE 60

Query: 187 EVEGFGKHTDISCVTILFQDEVGGLQMASKRGEWKDVRPVEDALVVNIGDFMQAWSNERL 246
            VEG G HTD+SC+TI++QD VGGLQM SK G+W D+ P  D LVVNIGD MQAWSN RL
Sbjct: 61  LVEGLGMHTDMSCITIVYQDSVGGLQMRSKEGKWIDINPCNDFLVVNIGDLMQAWSNGRL 120

Query: 247 RSAEHRVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGEGNSRLYKPFSIKEYRAFRET 306
           RS+EHRVVLR+ V R SLAFFL +E D+K I AP E+VGEG  R YK F   EY  FR++
Sbjct: 121 RSSEHRVVLRKLVNRVSLAFFLCFE-DEKVILAPQEIVGEGKQRSYKSFKCSEYLKFRQS 180

Query: 307 NYG---NIIGATLKDFA 316
           N       IG T+KDFA
Sbjct: 181 NEEGKFEKIGFTVKDFA 196

[Close]

BLAST of Tan0022657 vs. TAIR 10
Match: AT4G21200.1 (gibberellin 2-oxidase 8 )

HSP 1 Score: 147.9 bits (372), Expect = 1.4e-35
Identity = 98/301 (32.56%), Postives = 147/301 (48.84%), Query Frame = 0


Query: 11  ELPVID----MDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVADELLQL 70
           ELPVID    +D +E   E  + ++ RA  EWG F V NHG+S +   K+R     + + 
Sbjct: 41  ELPVIDVSRLIDGAEEEREKCKEAIARASREWGFFQVINHGISMDVLEKMRQEQIRVFRE 100

Query: 71  P--EETKGEKLKVG-ISWYTPRFKLSPYFETFKFFGPNFSASASEQGFTEALFGPRA--- 130
           P  +++K EK   G   W TP                  + S  +  ++EA   P     
Sbjct: 101 PFDKKSKSEKFSAGSYRWGTPS-----------------ATSIRQLSWSEAFHVPMTDIS 160

Query: 131 -----TELSNLLEEYGRKMTELSKRIVKLLLKTLGDNFENKFYDPEFSNCSGYLRINRYS 190
                T LS+ +E++  +   L+  + ++L +  G N  + F+       + YLR+NRY 
Sbjct: 161 DNKDFTTLSSTMEKFASESEALAYMLAEVLAEKSGQN--SSFFKENCVRNTCYLRMNRYP 220

Query: 191 PPDFDEEVEGFGKHTDISCVTILFQDEVGGLQMASKRGEWKDVRPVEDALVVNIGDFMQA 250
           P     EV G   HTD   +TIL+QD+VGGLQ+  K   W  V+P   AL++NIGD  QA
Sbjct: 221 PCPKPSEVYGLMPHTDSDFLTILYQDQVGGLQLI-KDNRWIAVKPNPKALIINIGDLFQA 280

Query: 251 WSNERLRSAEHRVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGEGNSRLYKPFSIKEY 297
           WSN   +S EHRV+    V+RFS A+F+    D       + +    +   Y+ FS +E+
Sbjct: 281 WSNGMYKSVEHRVMTNPKVERFSTAYFMCPSYD-------AVIECSSDRPAYRNFSFREF 314

[Close]

BLAST of Tan0022657 vs. TAIR 10
Match: AT1G52820.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 134.8 bits (338), Expect = 1.2e-31
Identity = 87/309 (28.16%), Postives = 150/309 (48.54%), Query Frame = 0


Query: 12  LPVID-----MDYSEHLSESSRSSLYRAFSEWGMFYVRNHGVSTEFYRKLRAVADELLQL 71
           LPVID     +   E   + +R+ + +A  ++G F      +  E  + +    +EL  L
Sbjct: 12  LPVIDFSNKNLKPGEPEWDLTRADVQKALQDYGYFEASFDRIPFELRKSVFGALEELFDL 71

Query: 72  PEETKGEKL-KVGISWYTPRFKLSPYFETFKFFGPNFSASASEQGFTEALFGPRATELSN 131
           P +TK   + K     Y  ++ + P +E+      + +       FTE L+       S 
Sbjct: 72  PLQTKLRNVSKKPFHGYVGQYPMVPLYESMGIDDSDIAEKV--DAFTEKLWPQGNISFST 131

Query: 132 LLEEYGRKMTELSKRIVKLLLKTLGDNFENKFYDPEFSNCSGYLRINRYSPPDFDEEVEG 191
            ++ + +K++EL   I ++++++ G    +K+ D    + +  LR+ +Y  PD +E   G
Sbjct: 132 TIQSFSKKLSELDITIRRMIMESFG---LDKYIDEHLHSTNYLLRVMKYKGPDTEETKVG 191

Query: 192 FGKHTDISCVTILFQDEVGGLQMASKRGEWKDVRPVEDALVVNIGDFMQAWSNERLRSAE 251
              HTD + VTIL+Q+ V GL++ +K   W  V+P +D+  V IGD + A  N RL S  
Sbjct: 192 LNAHTDKNIVTILYQNHVEGLEVQTKDKNWIKVKPTQDSFTVMIGDSLYALLNGRLHSPY 251

Query: 252 HRVVLRQNVKRFSLAFFLTYEDDDKEIFAPSEVVGEGNSRLYKPFSIKEYRAFRETNYGN 311
           HRV++     R+SL  F +       + +P E+V E + RL+KPF   E+  F  T  G 
Sbjct: 252 HRVMMTGTETRYSLGLF-SIPKAGHIVSSPDELVDEEHPRLFKPFDHVEFLQFYYTEAGQ 311

Query: 312 IIGATLKDF 315
              + LK +
Sbjct: 312 RSQSALKTY 314

The following BLAST results are available for this feature:

BLAST of Tan0022657 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4KBY0	2.6e-71	46.32	Gibberellin 20-oxidase-like protein OS=Arabidopsis thaliana OX=3702 GN=At5g51310...	[more]
O49561	2.0e-34	32.56	Gibberellin 2-beta-dioxygenase 8 OS=Arabidopsis thaliana OX=3702 GN=GA2OX8 PE=1 ...	[more]
Q8LGZ9	7.7e-31	31.51	Gibberellin 2-beta-dioxygenase 5 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
Q7XP65	3.8e-30	32.11	Gibberellin 2-beta-dioxygenase 6 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]
P0C5H5	9.4e-29	33.46	Gibberellin 20 oxidase 2 OS=Oryza sativa subsp. indica OX=39946 GN=20ox2 PE=1 SV...	[more]

BLAST of Tan0022657 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008453897.1	1.6e-129	70.35	PREDICTED: gibberellin 2-beta-dioxygenase 8 [Cucumis melo] >TYK16936.1 gibberell...	[more]
XP_038876311.1	7.7e-129	72.15	gibberellin 20-oxidase-like protein [Benincasa hispida]	[more]
KAA0044653.1	1.3e-128	69.72	gibberellin 2-beta-dioxygenase 8 [Cucumis melo var. makuwa]	[more]
XP_004146911.1	4.2e-127	70.03	gibberellin 20-oxidase-like protein [Cucumis sativus] >KGN53154.1 hypothetical p...	[more]
XP_022141254.1	1.8e-114	70.19	gibberellin 20-oxidase-like protein isoform X1 [Momordica charantia]	[more]

BLAST of Tan0022657 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3D2E7	7.5e-130	70.35	Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]
A0A1S3BYJ4	7.5e-130	70.35	gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo OX=3656 GN=LOC103494477 PE=3 SV...	[more]
A0A5A7TME4	6.4e-129	69.72	Gibberellin 2-beta-dioxygenase 8 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]
A0A0A0KW97	2.0e-127	70.03	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G...	[more]
A0A6J1CI26	6.8e-115	70.19	gibberellin 20-oxidase-like protein isoform X1 OS=Momordica charantia OX=3673 GN...	[more]

BLAST of Tan0022657 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23340.1	1.9e-80	50.93	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G51310.1	1.9e-72	46.32	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G23340.2	2.4e-59	58.38	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT4G21200.1	1.4e-35	32.56	gibberellin 2-oxidase 8	[more]
AT1G52820.1	1.2e-31	28.16	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 120..147
None	No IPR available	PANTHER	PTHR47990:SF9	GIBBERELLIN 20-OXIDASE-LIKE PROTEIN	coord: 8..305
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 8..305
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 10..303
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 12..72 e-value: 1.1E-11 score: 45.6
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 5..303 e-value: 1.4E-67 score: 230.4
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 170..263 e-value: 1.5E-23 score: 83.2
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 165..265 score: 12.125185

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0022657.1	Tan0022657.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0048767	root hair elongation
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding