Tan0022618 (gene) Snake gourd v1

Overview


Name	Tan0022618
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	subtilisin-like protease SBT3.11
Location	LG01: 77447780 .. 77456485 (-)
RNA-Seq Expression	Tan0022618
Synteny	Tan0022618

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCGTCTTAAAATGAGTATGAGTCTCCTGCTCGAAAATATTGTTGAATTAAAAAAGAAAATGGTGTATAATTACGGCCTACATAGGATCTCCTCGAAAACTTCTTAGGTTAGAGGGCATTTTGACAAATATGTAATTTGAAGAAAGGAATTGAATTTGAGCCCTAATTTCCCAATTTGAACCCAGCCCATCTGACAATTAACGAATCAAAACGATTGGCGTTCCCAAATACCAACCTTTTTTATCTACTCTTAACTGATACTCTCTATAATATCATGCACGAGTTGGAATGGCCAAGTTGAAATTCTCTTCATTTCCCCTGTACATCGTTTTGCTGTTCTCAATTTCTCTGAATTTGTTTCTTAGTTCTTCGGCAATGTCCGGCCAATCCGCCGATTCCAAGTCCGCTTCTCCGGCGGTTCATATCGTTTACACGGAGAGGCCTAGCGATGAGGAACCTGAGGTCTATCACATCCGAACCCTAGCTTCTGTTCTCGGCAGGTTTGTGTTTTACGAATGAGTATACTCCCTTTTGTGTGTCTTTTGCCTGAAAAATGTTGGAAATTCACTGTGGATTTTTGTTTAATTTGTTTATTTGTAGTGAAGAAGCTGCTCGGGAGGCTCTTGTGTATAGTTATAAGAACGCTGCCAGTGGCTTCTCTGCTCGGCTTACTCCCGATCAGGTTGCGGAGATTTCCAGTAAGTTGGTTGCAATTTTTCTCGTCGATTTAACTTAACAGATTGATTATTATCTGTGAAATGCTATATTATTTCGTCTATTTAGTGGATTTGTGGTATATATTAATGAATTTGAGAAAACTTTTGGGAAACTTCATTTTGGTTTGGCTTATTTGATTTTACCCAAACCTATACTTGAATGATTTTTAAAAAAATTTAGTTAGCCATGAGTGTGATTGTTGTTGGAAGAGACTTATTTTGTGTACTAGCCATTTCTTTTCTGTTGGATACTCTTTTGACTGGATTTGGGTATTTTATGTTGAATTTAGGTACTTTTTTATACGATTGATCTTGTAGCTTGATTTTAGTGTTTTCATCATTGGATTTGGACTTTTTTTTCATTATCGAAAGAGATATGCAAAAAAGGGAGATGAGATACCCACCACACCAAAGGTATTACAAATAGAACTCCCAATTGATAAAATTAGTTAAGCATCCCGACCCAGTTCCTCTCTGTCCTTGAAAGATTCTCTTGTTTCTCTTTTAAGTCGTACCACAATCTCCACCAAAGGGCTACAACCCCATTTATCCATAGATACCTCACTTGTCGTCAGAAAAGGGCCTTTGGCTCACGGAAAAAGCTGGGTGTTGTTGAAAATTTTGAAAAAAAAAAAGATCAAACCAGCAGGAAGCATCAAAGGTGCAGCTGGTTTTCTCTTTTAAGTGTCGTGTTGGTTGAATTTTGTGTAATTTATTAGTTGGTTCGTGGACAACTATCTAATTTAGTTTTGACTAACTAATGTAAATTTAAAATTTGAAGTTAATTAACTAACTAAGCCAAGTATGTGGATATAAAATGTTATGTTGACTTTCCTAGCTTTGATAGTTTCTAGTTGTGCTTTTCCAAACGTCTGGAGCTGCTAGGTTACTTGGTCTGATACCTGAAATGTGAGAAGCAATGTCAAACAAATTTTCTTACTCATTGAGTATGTTCTTGAAGTTGTTTGGTCCGGTAGTTAATTGGGATTTTGATTGTCCCTTTACTCTCTCACATGTTTGCTGTCCTCCTTAGTGCTCATAACATCTGGGTTAGCATGGGGGTCAAGAAGAAAGCTATAAACTCAGATATATTTTAGGTTATGGATTCAAATTAAAATGATGGTTGATAACTTGCTTAAAATATTGAATATCCTATAAAATTTTCAAAGCAAAATATTGTAAGATTAGGTAGTTGTCTTCCAGTATGGTTAATTTTGACCCATTGTGTAAGCTAGCTGAGGAATACTCATCGGGTTAATCTCCAAAGGCATATTTGGGGCTTAGGTTATAATAATCTGAGGAATACTCTGCATCCCAAACATAGACAACTATTATAACTCATGTTATTATAACCCACACAACACCCCAAACAACCCCTAAAAATTAGTGTCAGTTTAACATGATATCAAAGCAAGAGGTTCAAACTTGAGCCTTTGGATTCATTTCCTTCCCACTAATGTTAATAGTTTACAAGTTGGATTGTGTGCAACAAATTCATATTTGAACCCACACATGAGAGGGGTGTTAAATATATCATGATGTATCAACTTAATGGTAGTTTGACGTATTTACATTTACTTCTCACATCTCTACAACTTACATTAGTTCCTCAACAGTGTCGATTATGTATGTGTTGGTGTGAATATAGTTAGATATTCATGTTAGAATGTAATGAAATCCCCAGAATTACTACAAGGTACTGTTTCTTATGAACTTTTATCTTGGAACATGGCAGAACAAGAAGGAGTTTTACAAGTTGTCCCAAGCGGTACTGTTCGGCTGCATTCAGGACCTGCAGGGCTTCACTAGAAGAACATCGAACTCTTTCCCTGAAAATATGGCTCTTTTCAATGGTTTGAAAGTGCATACCTAAATAAAGTGTACTTTGTCTCTATTGTGCTTGCTTGAATAACCATATATCTTATATCTATTTGCTATACTATTATACTCATGTATCTATTTTCTATACCATAATGTGCGTGTTCGCACTTTTACTATCGATAACAAAGTCGATGGTTAACTAAAATAAACATGAGATGTAAGATTAGAATTCTTCCTCAAGCACCCTTCATTAAGGGAGTCTGGTGAGGTGTAGGTGCATGGCTTAGAATCGTTTTTCATACTAATCATGACTAAGTTTATACTTATCATGCCCAATTGGTTATGACTCTTAGTCGATTGAAAACGAGCAACTACATGGCATTCTTGAAAATTAACTAAAATAATATGTAAGATCAGAATTCTTCCTCCTACTCCTATATTTCTATGTATGCATTTATCTACAGAGTACTTTCTAG

mRNA sequence

CCGTCTTAAAATGAGTATGAGTCTCCTGCTCGAAAATATTGTTGAATTAAAAAAGAAAATGGTGTATAATTACGGCCTACATAGGATCTCCTCGAAAACTTCTTAGGTTAGAGGGCATTTTGACAAATATGTAATTTGAAGAAAGGAATTGAATTTGAGCCCTAATTTCCCAATTTGAACCCAGCCCATCTGACAATTAACGAATCAAAACGATTGGCGTTCCCAAATACCAACCTTTTTTATCTACTCTTAACTGATACTCTCTATAATATCATGCACGAGTTGGAATGGCCAAGTTGAAATTCTCTTCATTTCCCCTGTACATCGTTTTGCTGTTCTCAATTTCTCTGAATTTGTTTCTTAGTTCTTCGGCAATGTCCGGCCAATCCGCCGATTCCAAGTCCGCTTCTCCGGCGGTTCATATCGTTTACACGGAGAGGCCTAGCGATGAGGAACCTGAGGTCTATCACATCCGAACCCTAGCTTCTGTTCTCGGCAGTGAAGAAGCTGCTCGGGAGGCTCTTGTGTATAGTTATAAGAACGCTGCCAGTGGCTTCTCTGCTCGGCTTACTCCCGATCAGGTTGCGGAGATTTCCAAACAAGAAGGAGTTTTACAAGTTGTCCCAAGCGGTACTGTTCGGCTGCATTCAGGACCTGCAGGGCTTCACTAGAAGAACATCGAACTCTTTCCCTGAAAATATGGCTCTTTTCAATGGTTTGAAAGTGCATACCTAAATAAAGTGTACTTTGTCTCTATTGTGCTTGCTTGAATAACCATATATCTTATATCTATTTGCTATACTATTATACTCATGTATCTATTTTCTATACCATAATGTGCGTGTTCGCACTTTTACTATCGATAACAAAGTCGATGGTTAACTAAAATAAACATGAGATGTAAGATTAGAATTCTTCCTCAAGCACCCTTCATTAAGGGAGTCTGGTGAGGTGTAGGTGCATGGCTTAGAATCGTTTTTCATACTAATCATGACTAAGTTTATACTTATCATGCCCAATTGGTTATGACTCTTAGTCGATTGAAAACGAGCAACTACATGGCATTCTTGAAAATTAACTAAAATAATATGTAAGATCAGAATTCTTCCTCCTACTCCTATATTTCTATGTATGCATTTATCTACAGAGTACTTTCTAG

Coding sequence (CDS)

ATGGCCAAGTTGAAATTCTCTTCATTTCCCCTGTACATCGTTTTGCTGTTCTCAATTTCTCTGAATTTGTTTCTTAGTTCTTCGGCAATGTCCGGCCAATCCGCCGATTCCAAGTCCGCTTCTCCGGCGGTTCATATCGTTTACACGGAGAGGCCTAGCGATGAGGAACCTGAGGTCTATCACATCCGAACCCTAGCTTCTGTTCTCGGCAGTGAAGAAGCTGCTCGGGAGGCTCTTGTGTATAGTTATAAGAACGCTGCCAGTGGCTTCTCTGCTCGGCTTACTCCCGATCAGGTTGCGGAGATTTCCAAACAAGAAGGAGTTTTACAAGTTGTCCCAAGCGGTACTGTTCGGCTGCATTCAGGACCTGCAGGGCTTCACTAG

Protein sequence

MAKLKFSSFPLYIVLLFSISLNLFLSSSAMSGQSADSKSASPAVHIVYTERPSDEEPEVYHIRTLASVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISKQEGVLQVVPSGTVRLHSGPAGLH

Homology

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BLAST of Tan0022618 vs. ExPASy Swiss-Prot
Match: Q1PDX5 (Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana OX=3702 GN=SBT3.11 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 1.5e-09
Identity = 39/98 (39.80%), Postives = 54/98 (55.10%), Query Frame = 0


Query: 44  VHIVYTERPSDEEPEVY---HIRTLASVLGSEEAAREALVYSYKNAASGFSARLTPDQVA 103
           VHIVY       +PE+    H+R L S+LGS++ A E++V+SY+N  SGF+A LT  Q  
Sbjct: 37  VHIVYLGEKEHNDPELVTSSHLRMLESLLGSKKDASESIVHSYRNGFSGFAAHLTDSQAE 96

Query: 104 EISKQEGVLQVVPSGTVRL------------HSGPAGL 127
           +IS+   V+QV P+    L            HS P GL
Sbjct: 97  QISEHPDVVQVTPNTFYELQTTRTFDYLGLSHSTPKGL 134

[Close]

BLAST of Tan0022618 vs. ExPASy Swiss-Prot
Match: Q9C7U8 (Subtilisin-like protease SBT3.17 OS=Arabidopsis thaliana OX=3702 GN=SBT3.17 PE=3 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 3.3e-09
Identity = 34/79 (43.04%), Postives = 50/79 (63.29%), Query Frame = 0


Query: 44  VHIVYTERPSDEEPEVY---HIRTLASVLGSEEAAREALVYSYKNAASGFSARLTPDQVA 103
           +HIV+      + PE+    H + L  +LGS+EAA+ +LVY+YK+  SGF+A+LT  Q  
Sbjct: 37  IHIVHLGAKQHDTPELVTKSHYQILEPLLGSKEAAKNSLVYNYKHGFSGFAAKLTASQAK 96

Query: 104 EISKQEGVLQVVPSGTVRL 120
            +S    VL+VVPS  +RL
Sbjct: 97  NLSAHPEVLRVVPSRVMRL 115

[Close]

BLAST of Tan0022618 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 1.2e-08
Identity = 43/112 (38.39%), Postives = 62/112 (55.36%), Query Frame = 0


Query: 11  LYIVLLFSISLNLFLSSSAMSGQSADSKSASPAVHIVYTERPSDEEPEVY---HIRTLAS 70
           +Y+VL   I LN+  S  A S       SA   VHIVY      ++PE     H R L S
Sbjct: 8   IYVVLSLVIFLNVQRSFVAES-------SAKRKVHIVYLGEKQHDDPEFVTESHHRMLWS 67

Query: 71  VLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISKQEGVLQVVPSGTVRL 120
           +LGS+E A +++VYSY++  SGF+A+LT  Q  +I+    V+ V+P    +L
Sbjct: 68  LLGSKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKL 112

[Close]

BLAST of Tan0022618 vs. ExPASy Swiss-Prot
Match: Q8GUK4 (Subtilisin-like protease SBT3.13 OS=Arabidopsis thaliana OX=3702 GN=SBT3.13 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 2.1e-08
Identity = 41/118 (34.75%), Postives = 69/118 (58.47%), Query Frame = 0


Query: 13  IVLLFSISLNLFLSSSAMSGQSADSKSASPAVHIVYTERPSDEEPEVY---HIRTLASVL 72
           +VLL +I+L LFL++      +A +  +   V+IVY      ++PE+    H + L S+L
Sbjct: 10  LVLLLAIALVLFLNTELDFLTAAGALDSDSKVYIVYLGEREHDDPELVTASHHQMLESLL 69

Query: 73  GSEEAAREALVYSYKNAASGFSARLTPDQVAEISKQEGVLQVVPSGTVRLHSGPAGLH 128
            S+E A+ +L+YSY++  SGF+A LT  Q  +IS+   V+ V+P+   +L +  A  H
Sbjct: 70  QSKEDAQNSLIYSYQHGFSGFAALLTSSQAKKISEHPEVIHVIPNRIRKLKTTRAWDH 127

[Close]

BLAST of Tan0022618 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 4.7e-08
Identity = 32/84 (38.10%), Postives = 50/84 (59.52%), Query Frame = 0


Query: 39  SASPAVHIVYTERPSDEEPEVY---HIRTLASVLGSEEAAREALVYSYKNAASGFSARLT 98
           SA   VHIVY      ++P+     H + L+S+LGS++ A E++VYSY++  SGF+A+LT
Sbjct: 24  SAKSKVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKLT 83

Query: 99  PDQVAEISKQEGVLQVVPSGTVRL 120
             Q  +I+    V+ V+P     L
Sbjct: 84  KSQAKKIADSPEVIHVIPDSYYEL 107

[Close]

BLAST of Tan0022618 vs. NCBI nr
Match: XP_022935866.1 (subtilisin-like protease SBT3.11 [Cucurbita moschata] >XP_023537186.1 subtilisin-like protease SBT3.11 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 203.8 bits (517), Expect = 9.1e-49
Identity = 110/127 (86.61%), Postives = 115/127 (90.55%), Query Frame = 0


Query: 1   MAKLKFSSFPLYIVLLFSISLNLFLSSSAMSGQSADSKSASPAVHIVYTERPSDEEPEVY 60
           MAKL FSSFPL  VLLFSISLNL LSSSAMS QS DS SASPAVHIVY E+P DEEPE Y
Sbjct: 1   MAKLIFSSFPLCFVLLFSISLNLILSSSAMSAQSVDSASASPAVHIVYMEKPRDEEPEAY 60

Query: 61  HIRTLASVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISKQEGVLQVVPSGTVRLH 120
           HIRTLASVLGSEEAAREALVYSYKNAASGFSA+LTP+QVAE+SKQEGVLQVVPS T +LH
Sbjct: 61  HIRTLASVLGSEEAAREALVYSYKNAASGFSAKLTPNQVAEVSKQEGVLQVVPSRTYQLH 120

Query: 121 SGPAGLH 128
           SGPAGLH
Sbjct: 121 SGPAGLH 127

[Close]

BLAST of Tan0022618 vs. NCBI nr
Match: XP_038897393.1 (subtilisin-like protease SBT3.17 [Benincasa hispida])

HSP 1 Score: 188.0 bits (476), Expect = 5.2e-44
Identity = 103/127 (81.10%), Postives = 111/127 (87.40%), Query Frame = 0


Query: 1   MAKLKFSSFPLYIVLLFSISLNLFLSSSAMSGQSADSKSASPAVHIVYTERPSDEEPEVY 60
           MA L FSS    +VLLFS+SLNL L+SSAMS QS DS SASPAVHIVY E+P DEEPE Y
Sbjct: 1   MANLNFSS----LVLLFSLSLNLVLTSSAMSAQSTDSNSASPAVHIVYMEKPRDEEPEAY 60

Query: 61  HIRTLASVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISKQEGVLQVVPSGTVRLH 120
           HIRTL SVLGSEEAAREAL+YSYKNAASGFSARLTPDQVAEISKQEGVLQVVPS T++LH
Sbjct: 61  HIRTLTSVLGSEEAAREALLYSYKNAASGFSARLTPDQVAEISKQEGVLQVVPSQTLQLH 120

Query: 121 SGPAGLH 128
           SGP+ LH
Sbjct: 121 SGPSRLH 123

[Close]

BLAST of Tan0022618 vs. NCBI nr
Match: XP_022976948.1 (subtilisin-like protease SBT3.10 isoform X2 [Cucurbita maxima])

HSP 1 Score: 179.1 bits (453), Expect = 2.4e-41
Identity = 99/125 (79.20%), Postives = 107/125 (85.60%), Query Frame = 0


Query: 7   SSFPLYIVLLFSISLNLFLSSSAMSGQSAD----SKSASPAVHIVYTERPSDEEPEVYHI 66
           SSFP  +V +FSI LNLFLSS+ MS QSA     S  ASPAVHIVYTERP +EEPE YHI
Sbjct: 13  SSFPPILVFIFSIYLNLFLSSAVMSDQSASIPVVSNFASPAVHIVYTERPHNEEPEAYHI 72

Query: 67  RTLASVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISKQEGVLQVVPSGTVRLHSG 126
           RTLASVLGSEEAAREALVYSYKNAASGFSA+LTP+QVAE+SKQEGVLQVVPS T +LHSG
Sbjct: 73  RTLASVLGSEEAAREALVYSYKNAASGFSAKLTPNQVAEVSKQEGVLQVVPSRTYQLHSG 132

Query: 127 PAGLH 128
            AGLH
Sbjct: 133 SAGLH 137

[Close]

BLAST of Tan0022618 vs. NCBI nr
Match: XP_022143620.1 (subtilisin-like protease SBT3.11 isoform X1 [Momordica charantia])

HSP 1 Score: 172.9 bits (437), Expect = 1.7e-39
Identity = 97/126 (76.98%), Postives = 104/126 (82.54%), Query Frame = 0


Query: 1   MAKLKFSSFPLYIVLLFSISLNLFLSSSAMSGQSADSKSASPAVHIVYTERPSDEEPEVY 60
           MAK  FSSF L + LLFSISLN  LSS+AMSGQ      ASPAVHIVYTE+P DEEPE Y
Sbjct: 1   MAKTTFSSFLLCLALLFSISLNFLLSSAAMSGQ-----YASPAVHIVYTEKPRDEEPEAY 60

Query: 61  HIRTLASVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISKQEGVLQVVPSGTVRLH 120
           HIRTL SVLGSEEAA+EALVYSYKNAASGFSARLTPDQVAE+SKQ GVL VV SGT +LH
Sbjct: 61  HIRTLVSVLGSEEAAKEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLH 120

Query: 121 SGPAGL 127
           SG  G+
Sbjct: 121 SGGGGV 121

[Close]

BLAST of Tan0022618 vs. NCBI nr
Match: KAG7025071.1 (Subtilisin-like protease SBT3.11, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 171.0 bits (432), Expect = 6.5e-39
Identity = 88/101 (87.13%), Postives = 93/101 (92.08%), Query Frame = 0


Query: 27  SSAMSGQSADSKSASPAVHIVYTERPSDEEPEVYHIRTLASVLGSEEAAREALVYSYKNA 86
           SSAMS QS DS SASPAVHIVY E+P DEEPE YHIRTLASVLGSEEAAREALVYSYKNA
Sbjct: 4   SSAMSAQSVDSASASPAVHIVYMEKPRDEEPEAYHIRTLASVLGSEEAAREALVYSYKNA 63

Query: 87  ASGFSARLTPDQVAEISKQEGVLQVVPSGTVRLHSGPAGLH 128
           ASGFSA+LTP+QVAE+SKQEGVLQVVPS T +LHSGPAGLH
Sbjct: 64  ASGFSAKLTPNQVAEVSKQEGVLQVVPSRTYQLHSGPAGLH 104

[Close]

BLAST of Tan0022618 vs. ExPASy TrEMBL
Match: A0A6J1F5X6 (subtilisin-like protease SBT3.11 OS=Cucurbita moschata OX=3662 GN=LOC111442644 PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 4.4e-49
Identity = 110/127 (86.61%), Postives = 115/127 (90.55%), Query Frame = 0


Query: 1   MAKLKFSSFPLYIVLLFSISLNLFLSSSAMSGQSADSKSASPAVHIVYTERPSDEEPEVY 60
           MAKL FSSFPL  VLLFSISLNL LSSSAMS QS DS SASPAVHIVY E+P DEEPE Y
Sbjct: 1   MAKLIFSSFPLCFVLLFSISLNLILSSSAMSAQSVDSASASPAVHIVYMEKPRDEEPEAY 60

Query: 61  HIRTLASVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISKQEGVLQVVPSGTVRLH 120
           HIRTLASVLGSEEAAREALVYSYKNAASGFSA+LTP+QVAE+SKQEGVLQVVPS T +LH
Sbjct: 61  HIRTLASVLGSEEAAREALVYSYKNAASGFSAKLTPNQVAEVSKQEGVLQVVPSRTYQLH 120

Query: 121 SGPAGLH 128
           SGPAGLH
Sbjct: 121 SGPAGLH 127

[Close]

BLAST of Tan0022618 vs. ExPASy TrEMBL
Match: A0A6J1INL9 (subtilisin-like protease SBT3.10 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111477163 PE=4 SV=1)

HSP 1 Score: 179.1 bits (453), Expect = 1.2e-41
Identity = 99/125 (79.20%), Postives = 107/125 (85.60%), Query Frame = 0


Query: 7   SSFPLYIVLLFSISLNLFLSSSAMSGQSAD----SKSASPAVHIVYTERPSDEEPEVYHI 66
           SSFP  +V +FSI LNLFLSS+ MS QSA     S  ASPAVHIVYTERP +EEPE YHI
Sbjct: 13  SSFPPILVFIFSIYLNLFLSSAVMSDQSASIPVVSNFASPAVHIVYTERPHNEEPEAYHI 72

Query: 67  RTLASVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISKQEGVLQVVPSGTVRLHSG 126
           RTLASVLGSEEAAREALVYSYKNAASGFSA+LTP+QVAE+SKQEGVLQVVPS T +LHSG
Sbjct: 73  RTLASVLGSEEAAREALVYSYKNAASGFSAKLTPNQVAEVSKQEGVLQVVPSRTYQLHSG 132

Query: 127 PAGLH 128
            AGLH
Sbjct: 133 SAGLH 137

[Close]

BLAST of Tan0022618 vs. ExPASy TrEMBL
Match: A0A6J1CPA2 (subtilisin-like protease SBT3.11 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013481 PE=4 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 8.3e-40
Identity = 97/126 (76.98%), Postives = 104/126 (82.54%), Query Frame = 0


Query: 1   MAKLKFSSFPLYIVLLFSISLNLFLSSSAMSGQSADSKSASPAVHIVYTERPSDEEPEVY 60
           MAK  FSSF L + LLFSISLN  LSS+AMSGQ      ASPAVHIVYTE+P DEEPE Y
Sbjct: 1   MAKTTFSSFLLCLALLFSISLNFLLSSAAMSGQ-----YASPAVHIVYTEKPRDEEPEAY 60

Query: 61  HIRTLASVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISKQEGVLQVVPSGTVRLH 120
           HIRTL SVLGSEEAA+EALVYSYKNAASGFSARLTPDQVAE+SKQ GVL VV SGT +LH
Sbjct: 61  HIRTLVSVLGSEEAAKEALVYSYKNAASGFSARLTPDQVAEVSKQPGVLHVVQSGTHQLH 120

Query: 121 SGPAGL 127
           SG  G+
Sbjct: 121 SGGGGV 121

[Close]

BLAST of Tan0022618 vs. ExPASy TrEMBL
Match: A0A6J1F6S6 (subtilisin-like protease SBT3.13 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111442643 PE=4 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 7.0e-39
Identity = 95/124 (76.61%), Postives = 105/124 (84.68%), Query Frame = 0


Query: 7   SSFPLYIVLLFSISLNLFLSSSAMSGQSAD----SKSASPAVHIVYTERPSDEEPEVYHI 66
           SSFP  +VLLFSISLNLFLSS+ MS QSA     S  ASPAVHIVYTERP +EEPE YHI
Sbjct: 13  SSFPPILVLLFSISLNLFLSSAVMSDQSASIPVVSNFASPAVHIVYTERPHNEEPEAYHI 72

Query: 67  RTLASVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISKQEGVLQVVPSGTVRLHSG 126
           RTLASVLGSEEAAREALVYSYKNAASGFSARLTP+QVA+I+KQ GVL VV SG+ +LH+G
Sbjct: 73  RTLASVLGSEEAAREALVYSYKNAASGFSARLTPEQVADITKQPGVLHVVQSGSNKLHTG 132

[Close]

BLAST of Tan0022618 vs. ExPASy TrEMBL
Match: A0A6J1IH38 (subtilisin-like protease SBT3.6 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111477163 PE=4 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 2.3e-37
Identity = 92/123 (74.80%), Postives = 102/123 (82.93%), Query Frame = 0


Query: 7   SSFPLYIVLLFSISLNLFLSSSAMSGQSAD----SKSASPAVHIVYTERPSDEEPEVYHI 66
           SSFP  +V +FSI LNLFLSS+ MS QSA     S  ASPAVHIVYTERP +EEPE YHI
Sbjct: 13  SSFPPILVFIFSIYLNLFLSSAVMSDQSASIPVVSNFASPAVHIVYTERPHNEEPEAYHI 72

Query: 67  RTLASVLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISKQEGVLQVVPSGTVRLHSG 126
           RTLASVLGSEEAAREALVYSYKNAASGFSARLTP+QVA+I+KQ GVL VV SG+ +LH+G
Sbjct: 73  RTLASVLGSEEAAREALVYSYKNAASGFSARLTPEQVADITKQPGVLHVVQSGSNKLHTG 132

[Close]

BLAST of Tan0022618 vs. TAIR 10
Match: AT1G71950.1 (Proteinase inhibitor, propeptide )

HSP 1 Score: 126.3 bits (316), Expect = 1.7e-29
Identity = 68/117 (58.12%), Postives = 90/117 (76.92%), Query Frame = 0


Query: 11  LYIVLLFSISLNLFLSSSAMSGQ-SADSKSASPA-VHIVYTERPSDEEPEVYHIRTLASV 70
           + IV  F   L+ F +S  M+ + + ++  +S A VHI+YTE+P+DEEP+ YH+RTL+S 
Sbjct: 14  IIIVFFFFFCLSSFFASIVMADEYTGEATGSSEAKVHIIYTEKPTDEEPKTYHLRTLSSA 73

Query: 71  LGSEEAAREALVYSYKNAASGFSARLTPDQVAEISKQEGVLQVVPSGTVRLHSGPAG 126
           LGSEEAA++AL+YSYK AASGFSA+LTP+QVAEISKQ GV+QVVPS T +LH    G
Sbjct: 74  LGSEEAAKDALIYSYKEAASGFSAKLTPEQVAEISKQPGVIQVVPSQTYQLHKPGGG 130

[Close]

BLAST of Tan0022618 vs. TAIR 10
Match: AT5G11940.1 (Subtilase family protein )

HSP 1 Score: 63.9 bits (154), Expect = 1.0e-10
Identity = 39/98 (39.80%), Postives = 54/98 (55.10%), Query Frame = 0


Query: 44  VHIVYTERPSDEEPEVY---HIRTLASVLGSEEAAREALVYSYKNAASGFSARLTPDQVA 103
           VHIVY       +PE+    H+R L S+LGS++ A E++V+SY+N  SGF+A LT  Q  
Sbjct: 37  VHIVYLGEKEHNDPELVTSSHLRMLESLLGSKKDASESIVHSYRNGFSGFAAHLTDSQAE 96

Query: 104 EISKQEGVLQVVPSGTVRL------------HSGPAGL 127
           +IS+   V+QV P+    L            HS P GL
Sbjct: 97  QISEHPDVVQVTPNTFYELQTTRTFDYLGLSHSTPKGL 134

[Close]

BLAST of Tan0022618 vs. TAIR 10
Match: AT1G66220.1 (Subtilase family protein )

HSP 1 Score: 62.8 bits (151), Expect = 2.3e-10
Identity = 34/79 (43.04%), Postives = 50/79 (63.29%), Query Frame = 0


Query: 44  VHIVYTERPSDEEPEVY---HIRTLASVLGSEEAAREALVYSYKNAASGFSARLTPDQVA 103
           +HIV+      + PE+    H + L  +LGS+EAA+ +LVY+YK+  SGF+A+LT  Q  
Sbjct: 37  IHIVHLGAKQHDTPELVTKSHYQILEPLLGSKEAAKNSLVYNYKHGFSGFAAKLTASQAK 96

Query: 104 EISKQEGVLQVVPSGTVRL 120
            +S    VL+VVPS  +RL
Sbjct: 97  NLSAHPEVLRVVPSRVMRL 115

[Close]

BLAST of Tan0022618 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 60.8 bits (146), Expect = 8.9e-10
Identity = 43/112 (38.39%), Postives = 62/112 (55.36%), Query Frame = 0


Query: 11  LYIVLLFSISLNLFLSSSAMSGQSADSKSASPAVHIVYTERPSDEEPEVY---HIRTLAS 70
           +Y+VL   I LN+  S  A S       SA   VHIVY      ++PE     H R L S
Sbjct: 8   IYVVLSLVIFLNVQRSFVAES-------SAKRKVHIVYLGEKQHDDPEFVTESHHRMLWS 67

Query: 71  VLGSEEAAREALVYSYKNAASGFSARLTPDQVAEISKQEGVLQVVPSGTVRL 120
           +LGS+E A +++VYSY++  SGF+A+LT  Q  +I+    V+ V+P    +L
Sbjct: 68  LLGSKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKL 112

[Close]

BLAST of Tan0022618 vs. TAIR 10
Match: AT4G21650.1 (Subtilase family protein )

HSP 1 Score: 60.1 bits (144), Expect = 1.5e-09
Identity = 41/118 (34.75%), Postives = 69/118 (58.47%), Query Frame = 0


Query: 13  IVLLFSISLNLFLSSSAMSGQSADSKSASPAVHIVYTERPSDEEPEVY---HIRTLASVL 72
           +VLL +I+L LFL++      +A +  +   V+IVY      ++PE+    H + L S+L
Sbjct: 10  LVLLLAIALVLFLNTELDFLTAAGALDSDSKVYIVYLGEREHDDPELVTASHHQMLESLL 69

Query: 73  GSEEAAREALVYSYKNAASGFSARLTPDQVAEISKQEGVLQVVPSGTVRLHSGPAGLH 128
            S+E A+ +L+YSY++  SGF+A LT  Q  +IS+   V+ V+P+   +L +  A  H
Sbjct: 70  QSKEDAQNSLIYSYQHGFSGFAALLTSSQAKKISEHPEVIHVIPNRIRKLKTTRAWDH 127

The following BLAST results are available for this feature:

BLAST of Tan0022618 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q1PDX5	1.5e-09	39.80	Subtilisin-like protease SBT3.11 OS=Arabidopsis thaliana OX=3702 GN=SBT3.11 PE=2...	[more]
Q9C7U8	3.3e-09	43.04	Subtilisin-like protease SBT3.17 OS=Arabidopsis thaliana OX=3702 GN=SBT3.17 PE=3...	[more]
Q8L7I2	1.2e-08	38.39	Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 S...	[more]
Q8GUK4	2.1e-08	34.75	Subtilisin-like protease SBT3.13 OS=Arabidopsis thaliana OX=3702 GN=SBT3.13 PE=2...	[more]
F4HPF1	4.7e-08	38.10	Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 S...	[more]

BLAST of Tan0022618 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022935866.1	9.1e-49	86.61	subtilisin-like protease SBT3.11 [Cucurbita moschata] >XP_023537186.1 subtilisin...	[more]
XP_038897393.1	5.2e-44	81.10	subtilisin-like protease SBT3.17 [Benincasa hispida]	[more]
XP_022976948.1	2.4e-41	79.20	subtilisin-like protease SBT3.10 isoform X2 [Cucurbita maxima]	[more]
XP_022143620.1	1.7e-39	76.98	subtilisin-like protease SBT3.11 isoform X1 [Momordica charantia]	[more]
KAG7025071.1	6.5e-39	87.13	Subtilisin-like protease SBT3.11, partial [Cucurbita argyrosperma subsp. argyros...	[more]

BLAST of Tan0022618 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F5X6	4.4e-49	86.61	subtilisin-like protease SBT3.11 OS=Cucurbita moschata OX=3662 GN=LOC111442644 P...	[more]
A0A6J1INL9	1.2e-41	79.20	subtilisin-like protease SBT3.10 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1CPA2	8.3e-40	76.98	subtilisin-like protease SBT3.11 isoform X1 OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1F6S6	7.0e-39	76.61	subtilisin-like protease SBT3.13 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1IH38	2.3e-37	74.80	subtilisin-like protease SBT3.6 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]

BLAST of Tan0022618 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G71950.1	1.7e-29	58.12	Proteinase inhibitor, propeptide	[more]
AT5G11940.1	1.0e-10	39.80	Subtilase family protein	[more]
AT1G66220.1	2.3e-10	43.04	Subtilase family protein	[more]
AT4G10550.1	8.9e-10	38.39	Subtilase family protein	[more]
AT4G21650.1	1.5e-09	34.75	Subtilase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR037045	Peptidase S8 propeptide/proteinase inhibitor I9 superfamily	GENE3D	3.30.70.80	Peptidase S8 propeptide/proteinase inhibitor I9	coord: 15..109 e-value: 3.6E-23 score: 83.8
IPR010259	Peptidase S8 propeptide/proteinase inhibitor I9	PFAM	PF05922	Inhibitor_I9	coord: 34..109 e-value: 5.2E-13 score: 49.5
None	No IPR available	PANTHER	PTHR48222	PROTEINASE INHIBITOR, PROPEPTIDE	coord: 16..111

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0022618.1	Tan0022618.1	mRNA
Tan0022618.2	Tan0022618.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity
molecular_function	GO:0008233	peptidase activity