Tan0022574 (gene) Snake gourd v1

Overview
NameTan0022574
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionHigh-affinity nitrate transporter 2.2
LocationLG01: 41765865 .. 41767713 (-)
RNA-Seq ExpressionTan0022574
SyntenyTan0022574
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAGATTAAAGTAGTTCTTCTAAGTGTCGAGCAAAAGAAGAAAAAGAGATGTCTAATAACGTAGAGGGTTCTCCAGGAAGCTCGATGCATGGAGTGACTGGGAAAGAGCAAACATTTGCGTTCTCAGTTGCATCCCCAATAGTCCCAACTGATACAACTGCTAAATTTGCATTACCAGTTGATTCAGAACACAAGGCGAAAGTGTTCAAGCTTTTCTCATTAGCAAATCCACACATGAGAACCTTTCACCTTTCATGGATTTCATTTTTCACATGCTTCGTTTCGACATTTGCAGCAGCCCCACTTGTGCCCATCATCCGTGATAATCTAAACTTGACCAAAGTTGACATTGGTAACGCTGGTGTTACTTCTGTCTCTGGAAGTATATTCTCTCGTCTTGTTATGGGAGCTGTTTGTGACCTTTTAGGTCCTCGCTATGGCTGTGCTTTTCTCATTATGTTGTCTGCTCCCACTGTGTTTTGTATGTCTTTTGTTTCTTCTGCTGGTGGTTACATTGCTGTTAGATTCATGATTGGTTTCTCCCTTGCTACCTTTGTCTCCTGTCAATATTGGATGAGTACTATGTTCAACAGTCAAATCATTGGCCTTGTCAATGGTACTGCTGCTGGTTGGGGTAATATGGGTGGAGGAGCCACTCAACTCATTATGCCTTTGCTCTATGATATTATTAAGAGAGCTGGTGCTACTCCTTTCACTGCTTGGAGGATTGCTTTCTTTATCCCTGGTTCCCTTCATGTTATTATGGGCATTTTGGTTTTGACTCTTGGCCAAGACTTGCCCGATGGCAACCTTAGCAGCCTTCAGAAGAAGGGTAATGTTGCCAAAGATAAATTCTCAATGTAAGTGCACGGATTCTTCTTATTCTTTAATTTTTATTATTTATTTATTTAATCTGATCATGATGCATGGAACTAACTAGCTAGCTAGCTAATATATATAAATAAATATATGTGTTAGGTTCTATGGTATGCTGTGACTAATTACAGAACTTGGATCTTCGTCCTCCTGTATGGCTACTCAATGGGGGTTGAACTTTCCACGGATAATGTTATTGCTGAATATTTCTACGATAGGTAGGGTAATATTAAATTAATTAGAATTAATATTTGATAATCACTTGCTTGTTTGGTCTTAATTATAATATAATATAACAACACACAGGTTTGATCTAAAGCTGCACACGGCTGGGATTATAGCAGCAACATTCGGGATGGCTAATCTAGTGGCTCGACCCTTTGGAGGGTATGCATCGGACTACGCAGCTCGTTATTTCGGGATGAGAGGAAGACTATGGACTCTATGGATTCTCCAAACATTTGGGGGAGTGTTTTGCATGTGGCTTGGTAAAGCAAATAAGCTTCCAACAGCTATAGTAGCTATGATCTTGTTTTCAATTGGAGCTCAAGCCGCATGTGGAGCCACTTTTGGAATTATTCCCTTTATTTCTCGCCGCTCTTTGGGAATTATATCAGGTTTAACAGGAGCTGGTGGAAACTTTGGATCGGGTTTAACCCAATTAGTGTTCTTCTCAACCTCAAAATACTCGACAGATACAGGGTTGTTTTTGATGGGAATAATGATTGTTTGTTGCACTCTTCCAGTCACTTTGGTTCATTTTCCTCAATGGGGAAGCATGTTCTTACCACCAACTAAACACGTTGACAAATCAACTGAAGAATTCTACTATGGTTCTGAATGGACTGACGATGAAAAGAAGAAGGGTTTGCACCAACAAAGCATTAAGTTTGCTGAGAACAGCAGATCTGAACGTGGAAGGCGTGTTTCCTCAGCTCCAACTCCCCCCAATACCACTCCTTCTCATGTTTGA

mRNA sequence

AAAGATTAAAGTAGTTCTTCTAAGTGTCGAGCAAAAGAAGAAAAAGAGATGTCTAATAACGTAGAGGGTTCTCCAGGAAGCTCGATGCATGGAGTGACTGGGAAAGAGCAAACATTTGCGTTCTCAGTTGCATCCCCAATAGTCCCAACTGATACAACTGCTAAATTTGCATTACCAGTTGATTCAGAACACAAGGCGAAAGTGTTCAAGCTTTTCTCATTAGCAAATCCACACATGAGAACCTTTCACCTTTCATGGATTTCATTTTTCACATGCTTCGTTTCGACATTTGCAGCAGCCCCACTTGTGCCCATCATCCGTGATAATCTAAACTTGACCAAAGTTGACATTGGTAACGCTGGTGTTACTTCTGTCTCTGGAAGTATATTCTCTCGTCTTGTTATGGGAGCTGTTTGTGACCTTTTAGGTCCTCGCTATGGCTGTGCTTTTCTCATTATGTTGTCTGCTCCCACTGTGTTTTGTATGTCTTTTGTTTCTTCTGCTGGTGGTTACATTGCTGTTAGATTCATGATTGGTTTCTCCCTTGCTACCTTTGTCTCCTGTCAATATTGGATGAGTACTATGTTCAACAGTCAAATCATTGGCCTTGTCAATGGTACTGCTGCTGGTTGGGGTAATATGGGTGGAGGAGCCACTCAACTCATTATGCCTTTGCTCTATGATATTATTAAGAGAGCTGGTGCTACTCCTTTCACTGCTTGGAGGATTGCTTTCTTTATCCCTGGTTCCCTTCATGTTATTATGGGCATTTTGGTTTTGACTCTTGGCCAAGACTTGCCCGATGGCAACCTTAGCAGCCTTCAGAAGAAGGGTAATGTTCTATGGTATGCTGTGACTAATTACAGAACTTGGATCTTCGTCCTCCTGTATGGCTACTCAATGGGGGTTGAACTTTCCACGGATAATGTTATTGCTGAATATTTCTACGATAGGTTTGATCTAAAGCTGCACACGGCTGGGATTATAGCAGCAACATTCGGGATGGCTAATCTAGTGGCTCGACCCTTTGGAGGGTATGCATCGGACTACGCAGCTCGTTATTTCGGGATGAGAGGAAGACTATGGACTCTATGGATTCTCCAAACATTTGGGGGAGTGTTTTGCATGTGGCTTGGTAAAGCAAATAAGCTTCCAACAGCTATAGTAGCTATGATCTTGTTTTCAATTGGAGCTCAAGCCGCATGTGGAGCCACTTTTGGAATTATTCCCTTTATTTCTCGCCGCTCTTTGGGAATTATATCAGGTTTAACAGGAGCTGGTGGAAACTTTGGATCGGGTTTAACCCAATTAGTGTTCTTCTCAACCTCAAAATACTCGACAGATACAGGGTTGTTTTTGATGGGAATAATGATTGTTTGTTGCACTCTTCCAGTCACTTTGGTTCATTTTCCTCAATGGGGAAGCATGTTCTTACCACCAACTAAACACGTTGACAAATCAACTGAAGAATTCTACTATGGTTCTGAATGGACTGACGATGAAAAGAAGAAGGGTTTGCACCAACAAAGCATTAAGTTTGCTGAGAACAGCAGATCTGAACGTGGAAGGCGTGTTTCCTCAGCTCCAACTCCCCCCAATACCACTCCTTCTCATGTTTGA

Coding sequence (CDS)

ATGTCTAATAACGTAGAGGGTTCTCCAGGAAGCTCGATGCATGGAGTGACTGGGAAAGAGCAAACATTTGCGTTCTCAGTTGCATCCCCAATAGTCCCAACTGATACAACTGCTAAATTTGCATTACCAGTTGATTCAGAACACAAGGCGAAAGTGTTCAAGCTTTTCTCATTAGCAAATCCACACATGAGAACCTTTCACCTTTCATGGATTTCATTTTTCACATGCTTCGTTTCGACATTTGCAGCAGCCCCACTTGTGCCCATCATCCGTGATAATCTAAACTTGACCAAAGTTGACATTGGTAACGCTGGTGTTACTTCTGTCTCTGGAAGTATATTCTCTCGTCTTGTTATGGGAGCTGTTTGTGACCTTTTAGGTCCTCGCTATGGCTGTGCTTTTCTCATTATGTTGTCTGCTCCCACTGTGTTTTGTATGTCTTTTGTTTCTTCTGCTGGTGGTTACATTGCTGTTAGATTCATGATTGGTTTCTCCCTTGCTACCTTTGTCTCCTGTCAATATTGGATGAGTACTATGTTCAACAGTCAAATCATTGGCCTTGTCAATGGTACTGCTGCTGGTTGGGGTAATATGGGTGGAGGAGCCACTCAACTCATTATGCCTTTGCTCTATGATATTATTAAGAGAGCTGGTGCTACTCCTTTCACTGCTTGGAGGATTGCTTTCTTTATCCCTGGTTCCCTTCATGTTATTATGGGCATTTTGGTTTTGACTCTTGGCCAAGACTTGCCCGATGGCAACCTTAGCAGCCTTCAGAAGAAGGGTAATGTTCTATGGTATGCTGTGACTAATTACAGAACTTGGATCTTCGTCCTCCTGTATGGCTACTCAATGGGGGTTGAACTTTCCACGGATAATGTTATTGCTGAATATTTCTACGATAGGTTTGATCTAAAGCTGCACACGGCTGGGATTATAGCAGCAACATTCGGGATGGCTAATCTAGTGGCTCGACCCTTTGGAGGGTATGCATCGGACTACGCAGCTCGTTATTTCGGGATGAGAGGAAGACTATGGACTCTATGGATTCTCCAAACATTTGGGGGAGTGTTTTGCATGTGGCTTGGTAAAGCAAATAAGCTTCCAACAGCTATAGTAGCTATGATCTTGTTTTCAATTGGAGCTCAAGCCGCATGTGGAGCCACTTTTGGAATTATTCCCTTTATTTCTCGCCGCTCTTTGGGAATTATATCAGGTTTAACAGGAGCTGGTGGAAACTTTGGATCGGGTTTAACCCAATTAGTGTTCTTCTCAACCTCAAAATACTCGACAGATACAGGGTTGTTTTTGATGGGAATAATGATTGTTTGTTGCACTCTTCCAGTCACTTTGGTTCATTTTCCTCAATGGGGAAGCATGTTCTTACCACCAACTAAACACGTTGACAAATCAACTGAAGAATTCTACTATGGTTCTGAATGGACTGACGATGAAAAGAAGAAGGGTTTGCACCAACAAAGCATTAAGTTTGCTGAGAACAGCAGATCTGAACGTGGAAGGCGTGTTTCCTCAGCTCCAACTCCCCCCAATACCACTCCTTCTCATGTTTGA

Protein sequence

MSNNVEGSPGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCDLLGPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMGILVLTLGQDLPDGNLSSLQKKGNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQTFGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGLTQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKSTEEFYYGSEWTDDEKKKGLHQQSIKFAENSRSERGRRVSSAPTPPNTTPSHV
Homology
BLAST of Tan0022574 vs. ExPASy Swiss-Prot
Match: Q9FJH8 (High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 PE=2 SV=1)

HSP 1 Score: 847.8 bits (2189), Expect = 6.5e-245
Identity = 417/525 (79.43%), Postives = 466/525 (88.76%), Query Frame = 0

Query: 7   GSPGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHMRTF 66
           G PGSSMHGVTG+EQ++AFSV SP VP+D++AKF+LPVD+EHKAKVFKL S   PHMRTF
Sbjct: 6   GEPGSSMHGVTGREQSYAFSVESPAVPSDSSAKFSLPVDTEHKAKVFKLLSFEAPHMRTF 65

Query: 67  HLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCDLL 126
           HL+WISFFTCF+STFAAAPLVPIIRDNLNLT+ D+GNAGV SVSGSIFSRLVMGAVCDLL
Sbjct: 66  HLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNAGVASVSGSIFSRLVMGAVCDLL 125

Query: 127 GPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIG 186
           GPRYGCAFL+MLSAPTVF MSFV  AGGYI VRFMIGF LATFVSCQYWMSTMFN QIIG
Sbjct: 126 GPRYGCAFLVMLSAPTVFSMSFVGGAGGYITVRFMIGFCLATFVSCQYWMSTMFNGQIIG 185

Query: 187 LVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMGILVLTL 246
           LVNGTAAGWGNMGGG TQL+MP++Y+II+R G+T FTAWR+AFF+PG +H+IMGILVLTL
Sbjct: 186 LVNGTAAGWGNMGGGVTQLLMPMVYEIIRRLGSTSFTAWRMAFFVPGWMHIIMGILVLTL 245

Query: 247 GQDLPDGNLSSLQKKG--------NVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEY 306
           GQDLPDGN S+L+KKG         VLWYA+TNYRTW+FVLLYGYSMGVEL+TDNVIAEY
Sbjct: 246 GQDLPDGNRSTLEKKGAVTKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDNVIAEY 305

Query: 307 FYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQTFGGVF 366
           F+DRF LKLHTAGIIAA+FGMAN  ARP GG+ASD AAR FGMRGRLWTLWI+QT GG F
Sbjct: 306 FFDRFHLKLHTAGIIAASFGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQTLGGFF 365

Query: 367 CMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGL 426
           C+WLG+A  LPTA+V MILFS+GAQAACGATF IIPFISRRSLGIISGLTGAGGNFGSGL
Sbjct: 366 CLWLGRATTLPTAVVFMILFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGNFGSGL 425

Query: 427 TQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKSTEEFYYG 486
           TQLVFFSTS +ST+ GL  MG+MI+ CTLPVTLVHFPQWGSMFLP T+   KSTEE+YY 
Sbjct: 426 TQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTEEYYYM 485

Query: 487 SEWTDDEKKKGLHQQSIKFAENSRSERGRRVSSAPTPPNTTPSHV 524
            EWT+ EK+KG+H+ S+KFA NSRSERGRRV+SAP+PP   P HV
Sbjct: 486 KEWTETEKRKGMHEGSLKFAVNSRSERGRRVASAPSPP---PEHV 527

BLAST of Tan0022574 vs. ExPASy Swiss-Prot
Match: O82811 (High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 844.0 bits (2179), Expect = 9.4e-244
Identity = 417/525 (79.43%), Postives = 463/525 (88.19%), Query Frame = 0

Query: 7   GSPGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHMRTF 66
           G PGSSMHGVTG+EQ+FAFSV SPIV TD TAKF LPVD+EHKA VFKLFS A PHMRTF
Sbjct: 6   GEPGSSMHGVTGREQSFAFSVQSPIVHTDKTAKFDLPVDTEHKATVFKLFSFAKPHMRTF 65

Query: 67  HLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCDLL 126
           HLSWISF TCFVSTFAAAPLVPIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGAVCDLL
Sbjct: 66  HLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNAGVASVSGSIFSRLVMGAVCDLL 125

Query: 127 GPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIG 186
           GPRYGCAFL+MLSAPTVF MSFVS A G+I VRFMIGF LATFVSCQYWMSTMFNSQIIG
Sbjct: 126 GPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFNSQIIG 185

Query: 187 LVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMGILVLTL 246
           LVNGTAAGWGNMGGG TQL+MP++Y+II+R G+T FTAWRIAFF+PG LH+IMGILVL L
Sbjct: 186 LVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGILVLNL 245

Query: 247 GQDLPDGNLSSLQKKGNV--------LWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEY 306
           GQDLPDGN ++L+K G V        LWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEY
Sbjct: 246 GQDLPDGNRATLEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEY 305

Query: 307 FYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQTFGGVF 366
           F+DRF LKLHTAG+IAA FGMAN  ARP GGYASD+AA+YFGMRGRLWTLWI+QT GG+F
Sbjct: 306 FFDRFHLKLHTAGLIAACFGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQTAGGLF 365

Query: 367 CMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGL 426
           C+WLG+AN L TA+VAM+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGNFGSGL
Sbjct: 366 CVWLGRANTLVTAVVAMVLFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGNFGSGL 425

Query: 427 TQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKSTEEFYYG 486
           TQL+FFSTS ++T+ GL  MG+MIV CTLPVTLVHFPQWGSMFLPP+    K TE  YYG
Sbjct: 426 TQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTEAHYYG 485

Query: 487 SEWTDDEKKKGLHQQSIKFAENSRSERGRRVSSAPTPPNTTPSHV 524
           SEW + EK+K +HQ S++FAEN++SE GRRV SA TPP  TP++V
Sbjct: 486 SEWNEQEKQKNMHQGSLRFAENAKSEGGRRVRSAATPPENTPNNV 530

BLAST of Tan0022574 vs. ExPASy Swiss-Prot
Match: P0DKG9 (High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.1 PE=1 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 1.5e-233
Identity = 402/535 (75.14%), Postives = 453/535 (84.67%), Query Frame = 0

Query: 1   MSNNVEGSPGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLAN 60
           M ++  G+PGSS+HGVTG+E  FAFS           +KF LPVDSEHKAK  +L S AN
Sbjct: 1   MDSSTVGAPGSSLHGVTGREPAFAFSTEVGGEDAAAASKFDLPVDSEHKAKTIRLLSFAN 60

Query: 61  PHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMG 120
           PHMRTFHLSWISFF+CFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRL MG
Sbjct: 61  PHMRTFHLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMG 120

Query: 121 AVCDLLGPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMF 180
           A+CD+LGPRYGCAFLIML+APTVFCMS + SA GYIAVRF+IGFSLATFVSCQYWMSTMF
Sbjct: 121 AICDMLGPRYGCAFLIMLAAPTVFCMSLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMF 180

Query: 181 NSQIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMG 240
           NS+IIGLVNG AAGWGNMGGGATQLIMPL+YD+I++ GATPFTAWR+A+F+PG+LHV+MG
Sbjct: 181 NSKIIGLVNGLAAGWGNMGGGATQLIMPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMG 240

Query: 241 ILVLTLGQDLPDGNLSSLQKKGN--------VLWYAVTNYRTWIFVLLYGYSMGVELSTD 300
           +LVLTLGQDLPDGNL SLQKKG+        VLWYAVTNYRTWIFVLLYGYSMGVEL+TD
Sbjct: 241 VLVLTLGQDLPDGNLRSLQKKGDVNRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTD 300

Query: 301 NVIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQ 360
           NVIAEYFYDRFDL L  AGIIAA+FGMAN+VARP GG  SD  ARYFGMR RLW +WILQ
Sbjct: 301 NVIAEYFYDRFDLDLRVAGIIAASFGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQ 360

Query: 361 TFGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGG 420
           T GG FC+ LG+A+ LPT++V M+LFS  AQAACGA FG+IPF+SRRSLGIISG+TGAGG
Sbjct: 361 TAGGAFCLLLGRASTLPTSVVCMVLFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGG 420

Query: 421 NFGSGLTQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKST 480
           NFG+GLTQL+FF++S+YST TGL  MGIMI+ CTLPV LVHFPQWGSMFLPP    +   
Sbjct: 421 NFGAGLTQLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAE--- 480

Query: 481 EEFYYGSEWTDDEKKKGLHQQSIKFAENSRSERGRR-----VSSAPTPPNTTPSH 523
           EE YYGSEW++ EK KGLH  S+KFAENSRSERGRR      ++A TPPN +P H
Sbjct: 481 EEHYYGSEWSEQEKSKGLHGASLKFAENSRSERGRRNVINAAAAAATPPNNSPEH 532

BLAST of Tan0022574 vs. ExPASy Swiss-Prot
Match: P0DKH0 (High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 GN=NRT2.2 PE=1 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 1.5e-233
Identity = 402/535 (75.14%), Postives = 453/535 (84.67%), Query Frame = 0

Query: 1   MSNNVEGSPGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLAN 60
           M ++  G+PGSS+HGVTG+E  FAFS           +KF LPVDSEHKAK  +L S AN
Sbjct: 1   MDSSTVGAPGSSLHGVTGREPAFAFSTEVGGEDAAAASKFDLPVDSEHKAKTIRLLSFAN 60

Query: 61  PHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMG 120
           PHMRTFHLSWISFF+CFVSTFAAAPLVPIIRDNLNLTK DIGNAGV SVSGSIFSRL MG
Sbjct: 61  PHMRTFHLSWISFFSCFVSTFAAAPLVPIIRDNLNLTKADIGNAGVASVSGSIFSRLAMG 120

Query: 121 AVCDLLGPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMF 180
           A+CD+LGPRYGCAFLIML+APTVFCMS + SA GYIAVRF+IGFSLATFVSCQYWMSTMF
Sbjct: 121 AICDMLGPRYGCAFLIMLAAPTVFCMSLIDSAAGYIAVRFLIGFSLATFVSCQYWMSTMF 180

Query: 181 NSQIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMG 240
           NS+IIGLVNG AAGWGNMGGGATQLIMPL+YD+I++ GATPFTAWR+A+F+PG+LHV+MG
Sbjct: 181 NSKIIGLVNGLAAGWGNMGGGATQLIMPLVYDVIRKCGATPFTAWRLAYFVPGTLHVVMG 240

Query: 241 ILVLTLGQDLPDGNLSSLQKKGN--------VLWYAVTNYRTWIFVLLYGYSMGVELSTD 300
           +LVLTLGQDLPDGNL SLQKKG+        VLWYAVTNYRTWIFVLLYGYSMGVEL+TD
Sbjct: 241 VLVLTLGQDLPDGNLRSLQKKGDVNRDSFSRVLWYAVTNYRTWIFVLLYGYSMGVELTTD 300

Query: 301 NVIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQ 360
           NVIAEYFYDRFDL L  AGIIAA+FGMAN+VARP GG  SD  ARYFGMR RLW +WILQ
Sbjct: 301 NVIAEYFYDRFDLDLRVAGIIAASFGMANIVARPTGGLLSDLGARYFGMRARLWNIWILQ 360

Query: 361 TFGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGG 420
           T GG FC+ LG+A+ LPT++V M+LFS  AQAACGA FG+IPF+SRRSLGIISG+TGAGG
Sbjct: 361 TAGGAFCLLLGRASTLPTSVVCMVLFSFCAQAACGAIFGVIPFVSRRSLGIISGMTGAGG 420

Query: 421 NFGSGLTQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKST 480
           NFG+GLTQL+FF++S+YST TGL  MGIMI+ CTLPV LVHFPQWGSMFLPP    +   
Sbjct: 421 NFGAGLTQLLFFTSSRYSTGTGLEYMGIMIMACTLPVVLVHFPQWGSMFLPPNAGAE--- 480

Query: 481 EEFYYGSEWTDDEKKKGLHQQSIKFAENSRSERGRR-----VSSAPTPPNTTPSH 523
           EE YYGSEW++ EK KGLH  S+KFAENSRSERGRR      ++A TPPN +P H
Sbjct: 481 EEHYYGSEWSEQEKSKGLHGASLKFAENSRSERGRRNVINAAAAAATPPNNSPEH 532

BLAST of Tan0022574 vs. ExPASy Swiss-Prot
Match: Q9LMZ9 (High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana OX=3702 GN=NRT2.2 PE=2 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 1.3e-221
Identity = 384/509 (75.44%), Postives = 440/509 (86.44%), Query Frame = 0

Query: 9   PGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHMRTFHL 68
           PGSSMHGVTG+EQ++AFSV     PT+T  K+ LPVD+E KA VFKLFS A PHMRTFHL
Sbjct: 7   PGSSMHGVTGREQSYAFSVDGS-EPTNTKKKYNLPVDAEDKATVFKLFSFAKPHMRTFHL 66

Query: 69  SWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCDLLGP 128
           SWISF TCFVSTFAAAPL+PIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGAVCDLLGP
Sbjct: 67  SWISFSTCFVSTFAAAPLIPIIRENLNLTKHDIGNAGVASVSGSIFSRLVMGAVCDLLGP 126

Query: 129 RYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIGLV 188
           RYGCAFL+MLSAPTVF MSFVS A G+I VRFMIGF LATFVSCQYWMSTMFNSQIIGLV
Sbjct: 127 RYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFNSQIIGLV 186

Query: 189 NGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMGILVLTLGQ 248
           NGTAAGWGNMGGG TQL+MP++Y+II+R G+T FTAWRIAFF+PG LH+IMGILVLTLGQ
Sbjct: 187 NGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGILVLTLGQ 246

Query: 249 DLPDGNLSSLQKKGNV--------LWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYFY 308
           DLP GN ++++K G V        LWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYF+
Sbjct: 247 DLPGGNRAAMEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYFF 306

Query: 309 DRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQTFGGVFCM 368
           DRF LKLHTAGIIAA FGMAN  ARP GG+ASD AA+ FGMRGRLWTLWI+QT GG+FC+
Sbjct: 307 DRFHLKLHTAGIIAACFGMANFFARPAGGWASDIAAKRFGMRGRLWTLWIIQTSGGLFCV 366

Query: 369 WLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGLTQ 428
           WLG+AN L TA+V+M+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGNFGSGLTQ
Sbjct: 367 WLGRANTLVTAVVSMVLFSLGAQAACGATFAIVPFVSRRALGIISGLTGAGGNFGSGLTQ 426

Query: 429 LVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKSTEEFYYGSE 488
           LVFFSTS+++T+ GL  MG+MIV CTLPVTL+HFPQWGSMF PP+     +TE +Y G E
Sbjct: 427 LVFFSTSRFTTEEGLTWMGVMIVACTLPVTLIHFPQWGSMFFPPSNDSVDATEHYYVG-E 486

Query: 489 WTDDEKKKGLHQQSIKFAENSRSERGRRV 510
           ++ +E++ G+H +S  FA+ +++E G  V
Sbjct: 487 YSKEEQQIGMHLKSKLFADGAKTEGGSSV 513

BLAST of Tan0022574 vs. NCBI nr
Match: XP_038895485.1 (high affinity nitrate transporter 2.4-like [Benincasa hispida] >XP_038895611.1 high affinity nitrate transporter 2.4-like [Benincasa hispida])

HSP 1 Score: 1004.2 bits (2595), Expect = 4.1e-289
Identity = 503/528 (95.27%), Postives = 515/528 (97.54%), Query Frame = 0

Query: 4   NVEGSPGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHM 63
           +VEGSPGSSMHGVTG+EQTFAFSVASPIVPTDTTAKF+LPVDSEHKAKVFKL+SLANPHM
Sbjct: 3   DVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFSLPVDSEHKAKVFKLWSLANPHM 62

Query: 64  RTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVC 123
           RTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVC
Sbjct: 63  RTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVC 122

Query: 124 DLLGPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMFNSQ 183
           DLLGPRYGCAFLIMLSAPTVFCMSFVS+A GYIAVRFMIGFSLATFVSCQYWMSTMFNSQ
Sbjct: 123 DLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQ 182

Query: 184 IIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMGILV 243
           IIGLVNGTAAGWGNMGGGATQLIMPLLYD+I+RAGATPFTAWRIAFFIPG LHV+MGILV
Sbjct: 183 IIGLVNGTAAGWGNMGGGATQLIMPLLYDVIQRAGATPFTAWRIAFFIPGFLHVLMGILV 242

Query: 244 LTLGQDLPDGNLSSLQKKG--------NVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVI 303
           LTLGQDLPDGNL SLQKKG        NVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVI
Sbjct: 243 LTLGQDLPDGNLGSLQKKGNVTKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVI 302

Query: 304 AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQTFG 363
           AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQTFG
Sbjct: 303 AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTFG 362

Query: 364 GVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG 423
           GVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG
Sbjct: 363 GVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG 422

Query: 424 SGLTQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKSTEEF 483
           SGLTQLVFFST+KYSTDTGLF MGIMIVCCTLPVTLVHFPQWGSMFLPPTKHV+KSTEEF
Sbjct: 423 SGLTQLVFFSTTKYSTDTGLFFMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEF 482

Query: 484 YYGSEWTDDEKKKGLHQQSIKFAENSRSERGRRVSSAPTPPNTTPSHV 524
           YYGSEWT+DEKKKGLHQQSIKFAENSRSERGRRV+SAPTPPNTTPSHV
Sbjct: 483 YYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSHV 530

BLAST of Tan0022574 vs. NCBI nr
Match: XP_023520099.1 (high affinity nitrate transporter 2.4-like [Cucurbita pepo subsp. pepo] >XP_023520141.1 high affinity nitrate transporter 2.4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1004.2 bits (2595), Expect = 4.1e-289
Identity = 504/531 (94.92%), Postives = 514/531 (96.80%), Query Frame = 0

Query: 1   MSNNVEGSPGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLAN 60
           MS  VEGSPGSSMHGVTG+EQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFS AN
Sbjct: 1   MSGGVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFAN 60

Query: 61  PHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMG 120
           PHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMG
Sbjct: 61  PHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMG 120

Query: 121 AVCDLLGPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMF 180
           AVCDLLGPRYGCAFLIMLSAPTVFCMSFVS A GYIAVRFMIGFSLATFVSCQYWMSTMF
Sbjct: 121 AVCDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMF 180

Query: 181 NSQIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMG 240
           NSQIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPG LHVIMG
Sbjct: 181 NSQIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMG 240

Query: 241 ILVLTLGQDLPDGNLSSLQKKGN--------VLWYAVTNYRTWIFVLLYGYSMGVELSTD 300
           ILVLTLGQDLPDGNLSSLQKKG+        VLWYA+TNYRTWIFVLLYGYSMGVELSTD
Sbjct: 241 ILVLTLGQDLPDGNLSSLQKKGDVAKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTD 300

Query: 301 NVIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQ 360
           NVIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD+AARYFGMRGRLWTLWILQ
Sbjct: 301 NVIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQ 360

Query: 361 TFGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGG 420
           TFGGVFCMWLGKA+KLPTAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGG
Sbjct: 361 TFGGVFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGG 420

Query: 421 NFGSGLTQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKST 480
           NFGSGLTQLVFFST+KY+TD GLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHV+KST
Sbjct: 421 NFGSGLTQLVFFSTTKYTTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKST 480

Query: 481 EEFYYGSEWTDDEKKKGLHQQSIKFAENSRSERGRRVSSAPTPPNTTPSHV 524
           EEFYYG+EWT+DEKKKGLHQQSIKFAENSRSERGRRV SAPTPPNTTPSHV
Sbjct: 481 EEFYYGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 531

BLAST of Tan0022574 vs. NCBI nr
Match: XP_022964707.1 (high affinity nitrate transporter 2.4-like [Cucurbita moschata])

HSP 1 Score: 1002.3 bits (2590), Expect = 1.6e-288
Identity = 503/529 (95.09%), Postives = 513/529 (96.98%), Query Frame = 0

Query: 3   NNVEGSPGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPH 62
           + VEGSPGSSMHGVTG+EQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFS ANPH
Sbjct: 2   SGVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFANPH 61

Query: 63  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 122
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 123 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMFNS 182
           CDLLGPRYGCAFLIMLSAPTVFCMSFVS A GYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 183 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMGIL 242
           QIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPG LHVIMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMGIL 241

Query: 243 VLTLGQDLPDGNLSSLQKKGN--------VLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 302
           VLTLGQDLPDGNLSSLQKKG+        VLWYA+TNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLSSLQKKGDVAKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 303 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQTF 362
           IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD+AARYFGMRGRLWTLWILQTF
Sbjct: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTF 361

Query: 363 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 422
           GGVFCMWLGKA+KLPTAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421

Query: 423 GSGLTQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKSTEE 482
           GSGLTQLVFFST+KYSTD GLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHV+KSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 481

Query: 483 FYYGSEWTDDEKKKGLHQQSIKFAENSRSERGRRVSSAPTPPNTTPSHV 524
           FYYG+EWT+DEKKKGLHQQSIKFAENSRSERGRRV SAPTPPNTTPSHV
Sbjct: 482 FYYGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 530

BLAST of Tan0022574 vs. NCBI nr
Match: KAG6583556.1 (High-affinity nitrate transporter 2.1, partial [Cucurbita argyrosperma subsp. sororia] >KAG6583557.1 High-affinity nitrate transporter 2.1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1000.7 bits (2586), Expect = 4.6e-288
Identity = 502/529 (94.90%), Postives = 513/529 (96.98%), Query Frame = 0

Query: 3   NNVEGSPGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPH 62
           + VEGSPGSSMHGVTG+EQ+FAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFS ANPH
Sbjct: 2   SGVEGSPGSSMHGVTGREQSFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFANPH 61

Query: 63  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 122
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 123 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMFNS 182
           CDLLGPRYGCAFLIMLSAPTVFCMSFVS A GYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 183 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMGIL 242
           QIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPG LHVIMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMGIL 241

Query: 243 VLTLGQDLPDGNLSSLQKKGN--------VLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 302
           VLTLGQDLPDGNLSSLQKKG+        VLWYA+TNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLSSLQKKGDVAKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 303 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQTF 362
           IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD+AARYFGMRGRLWTLWILQTF
Sbjct: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTF 361

Query: 363 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 422
           GGVFCMWLGKA+KLPTAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421

Query: 423 GSGLTQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKSTEE 482
           GSGLTQLVFFST+KYSTD GLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHV+KSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 481

Query: 483 FYYGSEWTDDEKKKGLHQQSIKFAENSRSERGRRVSSAPTPPNTTPSHV 524
           FYYG+EWT+DEKKKGLHQQSIKFAENSRSERGRRV SAPTPPNTTPSHV
Sbjct: 482 FYYGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 530

BLAST of Tan0022574 vs. NCBI nr
Match: XP_022970277.1 (high affinity nitrate transporter 2.4-like [Cucurbita maxima] >XP_022970279.1 high affinity nitrate transporter 2.4-like [Cucurbita maxima] >XP_022970364.1 high affinity nitrate transporter 2.4-like [Cucurbita maxima])

HSP 1 Score: 997.7 bits (2578), Expect = 3.9e-287
Identity = 500/529 (94.52%), Postives = 512/529 (96.79%), Query Frame = 0

Query: 3   NNVEGSPGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPH 62
           + VEGSPGSSMHGVTG+EQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFS ANPH
Sbjct: 2   SGVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFANPH 61

Query: 63  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 122
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 123 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMFNS 182
           CDLLGPRYGCAFLIMLSAPTVFCMSFVS A GYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 183 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMGIL 242
           QIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPG LHVIMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMGIL 241

Query: 243 VLTLGQDLPDGNLSSLQKKGN--------VLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 302
           VLTLGQDLPDGNLSSLQKKG+        VLWYA+TNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLSSLQKKGDVTKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 303 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQTF 362
           IAEYFYDRFDLKL TAGIIAATFGMANLVARPFGGYASD+AARYFGMRGRLWTLWILQTF
Sbjct: 302 IAEYFYDRFDLKLQTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTF 361

Query: 363 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 422
           GGVFCMWLGKA+KLPTAI AMILFSIGAQAACGATFG+IPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGVIPFISRRSLGIISGLTGAGGNF 421

Query: 423 GSGLTQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKSTEE 482
           GSGLTQLVFFST+KYSTD GLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKH++KSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHLEKSTEE 481

Query: 483 FYYGSEWTDDEKKKGLHQQSIKFAENSRSERGRRVSSAPTPPNTTPSHV 524
           FYYG+EWT+DEKKKGLHQQSIKFAENSRSERGRRV SAPTPPNTTPSHV
Sbjct: 482 FYYGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 530

BLAST of Tan0022574 vs. ExPASy TrEMBL
Match: A0A6J1HIH1 (high affinity nitrate transporter 2.4-like OS=Cucurbita moschata OX=3662 GN=LOC111464708 PE=4 SV=1)

HSP 1 Score: 1002.3 bits (2590), Expect = 7.6e-289
Identity = 503/529 (95.09%), Postives = 513/529 (96.98%), Query Frame = 0

Query: 3   NNVEGSPGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPH 62
           + VEGSPGSSMHGVTG+EQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFS ANPH
Sbjct: 2   SGVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFANPH 61

Query: 63  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 122
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 123 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMFNS 182
           CDLLGPRYGCAFLIMLSAPTVFCMSFVS A GYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 183 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMGIL 242
           QIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPG LHVIMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMGIL 241

Query: 243 VLTLGQDLPDGNLSSLQKKGN--------VLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 302
           VLTLGQDLPDGNLSSLQKKG+        VLWYA+TNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLSSLQKKGDVAKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 303 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQTF 362
           IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD+AARYFGMRGRLWTLWILQTF
Sbjct: 302 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTF 361

Query: 363 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 422
           GGVFCMWLGKA+KLPTAI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 421

Query: 423 GSGLTQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKSTEE 482
           GSGLTQLVFFST+KYSTD GLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHV+KSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEE 481

Query: 483 FYYGSEWTDDEKKKGLHQQSIKFAENSRSERGRRVSSAPTPPNTTPSHV 524
           FYYG+EWT+DEKKKGLHQQSIKFAENSRSERGRRV SAPTPPNTTPSHV
Sbjct: 482 FYYGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 530

BLAST of Tan0022574 vs. ExPASy TrEMBL
Match: A0A6J1HYN4 (high affinity nitrate transporter 2.4-like OS=Cucurbita maxima OX=3661 GN=LOC111469291 PE=4 SV=1)

HSP 1 Score: 997.7 bits (2578), Expect = 1.9e-287
Identity = 500/529 (94.52%), Postives = 512/529 (96.79%), Query Frame = 0

Query: 3   NNVEGSPGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPH 62
           + VEGSPGSSMHGVTG+EQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFS ANPH
Sbjct: 2   SGVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSFANPH 61

Query: 63  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 122
           MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV
Sbjct: 62  MRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAV 121

Query: 123 CDLLGPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMFNS 182
           CDLLGPRYGCAFLIMLSAPTVFCMSFVS A GYIAVRFMIGFSLATFVSCQYWMSTMFNS
Sbjct: 122 CDLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFNS 181

Query: 183 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMGIL 242
           QIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPG LHVIMGIL
Sbjct: 182 QIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGWLHVIMGIL 241

Query: 243 VLTLGQDLPDGNLSSLQKKGN--------VLWYAVTNYRTWIFVLLYGYSMGVELSTDNV 302
           VLTLGQDLPDGNLSSLQKKG+        VLWYA+TNYRTWIFVLLYGYSMGVELSTDNV
Sbjct: 242 VLTLGQDLPDGNLSSLQKKGDVTKDKFSKVLWYAITNYRTWIFVLLYGYSMGVELSTDNV 301

Query: 303 IAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQTF 362
           IAEYFYDRFDLKL TAGIIAATFGMANLVARPFGGYASD+AARYFGMRGRLWTLWILQTF
Sbjct: 302 IAEYFYDRFDLKLQTAGIIAATFGMANLVARPFGGYASDFAARYFGMRGRLWTLWILQTF 361

Query: 363 GGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNF 422
           GGVFCMWLGKA+KLPTAI AMILFSIGAQAACGATFG+IPFISRRSLGIISGLTGAGGNF
Sbjct: 362 GGVFCMWLGKADKLPTAIAAMILFSIGAQAACGATFGVIPFISRRSLGIISGLTGAGGNF 421

Query: 423 GSGLTQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKSTEE 482
           GSGLTQLVFFST+KYSTD GLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKH++KSTEE
Sbjct: 422 GSGLTQLVFFSTTKYSTDKGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHLEKSTEE 481

Query: 483 FYYGSEWTDDEKKKGLHQQSIKFAENSRSERGRRVSSAPTPPNTTPSHV 524
           FYYG+EWT+DEKKKGLHQQSIKFAENSRSERGRRV SAPTPPNTTPSHV
Sbjct: 482 FYYGAEWTEDEKKKGLHQQSIKFAENSRSERGRRVGSAPTPPNTTPSHV 530

BLAST of Tan0022574 vs. ExPASy TrEMBL
Match: A0A3Q9CZ42 (High affinity nitrate transporter 2.1 OS=Cucumis sativus OX=3659 GN=NRT2.1 PE=2 SV=1)

HSP 1 Score: 996.5 bits (2575), Expect = 4.2e-287
Identity = 498/528 (94.32%), Postives = 514/528 (97.35%), Query Frame = 0

Query: 4   NVEGSPGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHM 63
           +VEGSPGSSMHGVTG+EQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVF+++SLANPHM
Sbjct: 3   DVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANPHM 62

Query: 64  RTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVC 123
           RTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVC
Sbjct: 63  RTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVC 122

Query: 124 DLLGPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMFNSQ 183
           DLLGPRYGCAFLIMLSAPTVFCMSFVS+A GYIAVRFMIGFSLATFVSCQYWMSTMFNSQ
Sbjct: 123 DLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQ 182

Query: 184 IIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMGILV 243
           IIGLVNGTAAGWGNMGGGATQLIMPLLY++I+RAGATPFTAWRIAFFIPG LHV+MGILV
Sbjct: 183 IIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGILV 242

Query: 244 LTLGQDLPDGNLSSLQKKG--------NVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVI 303
           LTLGQDLPDGNL+SLQKKG        NVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVI
Sbjct: 243 LTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVI 302

Query: 304 AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQTFG 363
           AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQTFG
Sbjct: 303 AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTFG 362

Query: 364 GVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG 423
           GVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG
Sbjct: 363 GVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG 422

Query: 424 SGLTQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKSTEEF 483
           SGLTQLVFFST+KYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHV+KSTEEF
Sbjct: 423 SGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEF 482

Query: 484 YYGSEWTDDEKKKGLHQQSIKFAENSRSERGRRVSSAPTPPNTTPSHV 524
           YYGSEWT+DEKKKGLHQQSIKFAENSRSERGRRV+SAPTPPNTTP+HV
Sbjct: 483 YYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

BLAST of Tan0022574 vs. ExPASy TrEMBL
Match: S4VM42 (High-affinity nitrate transporter NRT2.2 OS=Cucumis sativus OX=3659 GN=NRT2.2 PE=2 SV=1)

HSP 1 Score: 995.7 bits (2573), Expect = 7.1e-287
Identity = 497/528 (94.13%), Postives = 513/528 (97.16%), Query Frame = 0

Query: 4   NVEGSPGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHM 63
           +VEGSPGSSMHGVTG+EQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVF+++SLANPHM
Sbjct: 3   DVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANPHM 62

Query: 64  RTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVC 123
           RTFHLSW+SFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVC
Sbjct: 63  RTFHLSWVSFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVC 122

Query: 124 DLLGPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMFNSQ 183
           DLLGPRYGCAFLIMLSAPTVFCMSFVS A GYIAVRFMIGFSLATFVSCQYWMSTMFNSQ
Sbjct: 123 DLLGPRYGCAFLIMLSAPTVFCMSFVSDAAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQ 182

Query: 184 IIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMGILV 243
           IIGLVNGTAAGWGNMGGGATQLIMPLLY++I+RAGATPFTAWRIAFFIPG LHV+MGILV
Sbjct: 183 IIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGILV 242

Query: 244 LTLGQDLPDGNLSSLQKKG--------NVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVI 303
           LTLGQDLPDGNL+SLQKKG        NVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVI
Sbjct: 243 LTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVI 302

Query: 304 AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQTFG 363
           AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQTFG
Sbjct: 303 AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTFG 362

Query: 364 GVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG 423
           GVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG
Sbjct: 363 GVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG 422

Query: 424 SGLTQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKSTEEF 483
           SGLTQLVFFST+KYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHV+KSTEEF
Sbjct: 423 SGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEF 482

Query: 484 YYGSEWTDDEKKKGLHQQSIKFAENSRSERGRRVSSAPTPPNTTPSHV 524
           YYGSEWT+DEKKKGLHQQSIKFAENSRSERGRRV+SAPTPPNTTP+HV
Sbjct: 483 YYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPTHV 530

BLAST of Tan0022574 vs. ExPASy TrEMBL
Match: Q6PRP8 (High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT2 PE=2 SV=3)

HSP 1 Score: 994.6 bits (2570), Expect = 1.6e-286
Identity = 498/528 (94.32%), Postives = 513/528 (97.16%), Query Frame = 0

Query: 4   NVEGSPGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHM 63
           +VEGSPGSSMHGVTG+EQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVF+++SLANPHM
Sbjct: 3   DVEGSPGSSMHGVTGREQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFRIWSLANPHM 62

Query: 64  RTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVC 123
           RTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVC
Sbjct: 63  RTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVC 122

Query: 124 DLLGPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMFNSQ 183
           DLLGPRYGCAFLIMLSAPTVFCMSFVS+A GYIAVRFMIGFSLATFVSCQYWMSTMFNSQ
Sbjct: 123 DLLGPRYGCAFLIMLSAPTVFCMSFVSNAAGYIAVRFMIGFSLATFVSCQYWMSTMFNSQ 182

Query: 184 IIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMGILV 243
           IIGLVNGTAAGWGNMGGGATQLIMPLLY++I+RAGATPFTAWRIAFFIPG LHV+MGILV
Sbjct: 183 IIGLVNGTAAGWGNMGGGATQLIMPLLYEVIQRAGATPFTAWRIAFFIPGFLHVLMGILV 242

Query: 244 LTLGQDLPDGNLSSLQKKG--------NVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVI 303
           LTLGQDLPDGNL+SLQKKG        NVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVI
Sbjct: 243 LTLGQDLPDGNLASLQKKGNVAKDKFSNVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVI 302

Query: 304 AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQTFG 363
           AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASD AARYFGMRGRLWTLWILQTFG
Sbjct: 303 AEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDVAARYFGMRGRLWTLWILQTFG 362

Query: 364 GVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG 423
           GVFCMWLGKA KLP AI AMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG
Sbjct: 363 GVFCMWLGKATKLPIAIAAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFG 422

Query: 424 SGLTQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKSTEEF 483
           SGLTQLVFFST+KYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHV+KSTEEF
Sbjct: 423 SGLTQLVFFSTTKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVEKSTEEF 482

Query: 484 YYGSEWTDDEKKKGLHQQSIKFAENSRSERGRRVSSAPTPPNTTPSHV 524
           YYGSEWT+DEKKKGLHQQSIKFAENSRSERGRRV+SAPTPPNTTPS V
Sbjct: 483 YYGSEWTEDEKKKGLHQQSIKFAENSRSERGRRVASAPTPPNTTPSRV 530

BLAST of Tan0022574 vs. TAIR 10
Match: AT5G60770.1 (nitrate transporter 2.4 )

HSP 1 Score: 847.8 bits (2189), Expect = 4.6e-246
Identity = 417/525 (79.43%), Postives = 466/525 (88.76%), Query Frame = 0

Query: 7   GSPGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHMRTF 66
           G PGSSMHGVTG+EQ++AFSV SP VP+D++AKF+LPVD+EHKAKVFKL S   PHMRTF
Sbjct: 6   GEPGSSMHGVTGREQSYAFSVESPAVPSDSSAKFSLPVDTEHKAKVFKLLSFEAPHMRTF 65

Query: 67  HLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCDLL 126
           HL+WISFFTCF+STFAAAPLVPIIRDNLNLT+ D+GNAGV SVSGSIFSRLVMGAVCDLL
Sbjct: 66  HLAWISFFTCFISTFAAAPLVPIIRDNLNLTRQDVGNAGVASVSGSIFSRLVMGAVCDLL 125

Query: 127 GPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIG 186
           GPRYGCAFL+MLSAPTVF MSFV  AGGYI VRFMIGF LATFVSCQYWMSTMFN QIIG
Sbjct: 126 GPRYGCAFLVMLSAPTVFSMSFVGGAGGYITVRFMIGFCLATFVSCQYWMSTMFNGQIIG 185

Query: 187 LVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMGILVLTL 246
           LVNGTAAGWGNMGGG TQL+MP++Y+II+R G+T FTAWR+AFF+PG +H+IMGILVLTL
Sbjct: 186 LVNGTAAGWGNMGGGVTQLLMPMVYEIIRRLGSTSFTAWRMAFFVPGWMHIIMGILVLTL 245

Query: 247 GQDLPDGNLSSLQKKG--------NVLWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEY 306
           GQDLPDGN S+L+KKG         VLWYA+TNYRTW+FVLLYGYSMGVEL+TDNVIAEY
Sbjct: 246 GQDLPDGNRSTLEKKGAVTKDKFSKVLWYAITNYRTWVFVLLYGYSMGVELTTDNVIAEY 305

Query: 307 FYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQTFGGVF 366
           F+DRF LKLHTAGIIAA+FGMAN  ARP GG+ASD AAR FGMRGRLWTLWI+QT GG F
Sbjct: 306 FFDRFHLKLHTAGIIAASFGMANFFARPIGGWASDIAARRFGMRGRLWTLWIIQTLGGFF 365

Query: 367 CMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGL 426
           C+WLG+A  LPTA+V MILFS+GAQAACGATF IIPFISRRSLGIISGLTGAGGNFGSGL
Sbjct: 366 CLWLGRATTLPTAVVFMILFSLGAQAACGATFAIIPFISRRSLGIISGLTGAGGNFGSGL 425

Query: 427 TQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKSTEEFYYG 486
           TQLVFFSTS +ST+ GL  MG+MI+ CTLPVTLVHFPQWGSMFLP T+   KSTEE+YY 
Sbjct: 426 TQLVFFSTSTFSTEQGLTWMGVMIMACTLPVTLVHFPQWGSMFLPSTEDEVKSTEEYYYM 485

Query: 487 SEWTDDEKKKGLHQQSIKFAENSRSERGRRVSSAPTPPNTTPSHV 524
            EWT+ EK+KG+H+ S+KFA NSRSERGRRV+SAP+PP   P HV
Sbjct: 486 KEWTETEKRKGMHEGSLKFAVNSRSERGRRVASAPSPP---PEHV 527

BLAST of Tan0022574 vs. TAIR 10
Match: AT1G08090.1 (nitrate transporter 2:1 )

HSP 1 Score: 844.0 bits (2179), Expect = 6.6e-245
Identity = 417/525 (79.43%), Postives = 463/525 (88.19%), Query Frame = 0

Query: 7   GSPGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHMRTF 66
           G PGSSMHGVTG+EQ+FAFSV SPIV TD TAKF LPVD+EHKA VFKLFS A PHMRTF
Sbjct: 6   GEPGSSMHGVTGREQSFAFSVQSPIVHTDKTAKFDLPVDTEHKATVFKLFSFAKPHMRTF 65

Query: 67  HLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCDLL 126
           HLSWISF TCFVSTFAAAPLVPIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGAVCDLL
Sbjct: 66  HLSWISFSTCFVSTFAAAPLVPIIRENLNLTKQDIGNAGVASVSGSIFSRLVMGAVCDLL 125

Query: 127 GPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIG 186
           GPRYGCAFL+MLSAPTVF MSFVS A G+I VRFMIGF LATFVSCQYWMSTMFNSQIIG
Sbjct: 126 GPRYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFNSQIIG 185

Query: 187 LVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMGILVLTL 246
           LVNGTAAGWGNMGGG TQL+MP++Y+II+R G+T FTAWRIAFF+PG LH+IMGILVL L
Sbjct: 186 LVNGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGILVLNL 245

Query: 247 GQDLPDGNLSSLQKKGNV--------LWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEY 306
           GQDLPDGN ++L+K G V        LWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEY
Sbjct: 246 GQDLPDGNRATLEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEY 305

Query: 307 FYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQTFGGVF 366
           F+DRF LKLHTAG+IAA FGMAN  ARP GGYASD+AA+YFGMRGRLWTLWI+QT GG+F
Sbjct: 306 FFDRFHLKLHTAGLIAACFGMANFFARPAGGYASDFAAKYFGMRGRLWTLWIIQTAGGLF 365

Query: 367 CMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGL 426
           C+WLG+AN L TA+VAM+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGNFGSGL
Sbjct: 366 CVWLGRANTLVTAVVAMVLFSMGAQAACGATFAIVPFVSRRALGIISGLTGAGGNFGSGL 425

Query: 427 TQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKSTEEFYYG 486
           TQL+FFSTS ++T+ GL  MG+MIV CTLPVTLVHFPQWGSMFLPP+    K TE  YYG
Sbjct: 426 TQLLFFSTSHFTTEQGLTWMGVMIVACTLPVTLVHFPQWGSMFLPPSTDPVKGTEAHYYG 485

Query: 487 SEWTDDEKKKGLHQQSIKFAENSRSERGRRVSSAPTPPNTTPSHV 524
           SEW + EK+K +HQ S++FAEN++SE GRRV SA TPP  TP++V
Sbjct: 486 SEWNEQEKQKNMHQGSLRFAENAKSEGGRRVRSAATPPENTPNNV 530

BLAST of Tan0022574 vs. TAIR 10
Match: AT1G08100.1 (nitrate transporter 2.2 )

HSP 1 Score: 770.4 bits (1988), Expect = 9.3e-223
Identity = 384/509 (75.44%), Postives = 440/509 (86.44%), Query Frame = 0

Query: 9   PGSSMHGVTGKEQTFAFSVASPIVPTDTTAKFALPVDSEHKAKVFKLFSLANPHMRTFHL 68
           PGSSMHGVTG+EQ++AFSV     PT+T  K+ LPVD+E KA VFKLFS A PHMRTFHL
Sbjct: 7   PGSSMHGVTGREQSYAFSVDGS-EPTNTKKKYNLPVDAEDKATVFKLFSFAKPHMRTFHL 66

Query: 69  SWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVMGAVCDLLGP 128
           SWISF TCFVSTFAAAPL+PIIR+NLNLTK DIGNAGV SVSGSIFSRLVMGAVCDLLGP
Sbjct: 67  SWISFSTCFVSTFAAAPLIPIIRENLNLTKHDIGNAGVASVSGSIFSRLVMGAVCDLLGP 126

Query: 129 RYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTMFNSQIIGLV 188
           RYGCAFL+MLSAPTVF MSFVS A G+I VRFMIGF LATFVSCQYWMSTMFNSQIIGLV
Sbjct: 127 RYGCAFLVMLSAPTVFSMSFVSDAAGFITVRFMIGFCLATFVSCQYWMSTMFNSQIIGLV 186

Query: 189 NGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIMGILVLTLGQ 248
           NGTAAGWGNMGGG TQL+MP++Y+II+R G+T FTAWRIAFF+PG LH+IMGILVLTLGQ
Sbjct: 187 NGTAAGWGNMGGGITQLLMPIVYEIIRRCGSTAFTAWRIAFFVPGWLHIIMGILVLTLGQ 246

Query: 249 DLPDGNLSSLQKKGNV--------LWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYFY 308
           DLP GN ++++K G V        LWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYF+
Sbjct: 247 DLPGGNRAAMEKAGEVAKDKFGKILWYAVTNYRTWIFVLLYGYSMGVELSTDNVIAEYFF 306

Query: 309 DRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWILQTFGGVFCM 368
           DRF LKLHTAGIIAA FGMAN  ARP GG+ASD AA+ FGMRGRLWTLWI+QT GG+FC+
Sbjct: 307 DRFHLKLHTAGIIAACFGMANFFARPAGGWASDIAAKRFGMRGRLWTLWIIQTSGGLFCV 366

Query: 369 WLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAGGNFGSGLTQ 428
           WLG+AN L TA+V+M+LFS+GAQAACGATF I+PF+SRR+LGIISGLTGAGGNFGSGLTQ
Sbjct: 367 WLGRANTLVTAVVSMVLFSLGAQAACGATFAIVPFVSRRALGIISGLTGAGGNFGSGLTQ 426

Query: 429 LVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKSTEEFYYGSE 488
           LVFFSTS+++T+ GL  MG+MIV CTLPVTL+HFPQWGSMF PP+     +TE +Y G E
Sbjct: 427 LVFFSTSRFTTEEGLTWMGVMIVACTLPVTLIHFPQWGSMFFPPSNDSVDATEHYYVG-E 486

Query: 489 WTDDEKKKGLHQQSIKFAENSRSERGRRV 510
           ++ +E++ G+H +S  FA+ +++E G  V
Sbjct: 487 YSKEEQQIGMHLKSKLFADGAKTEGGSSV 513

BLAST of Tan0022574 vs. TAIR 10
Match: AT5G60780.1 (nitrate transporter 2.3 )

HSP 1 Score: 766.9 bits (1979), Expect = 1.0e-221
Identity = 380/534 (71.16%), Postives = 443/534 (82.96%), Query Frame = 0

Query: 3   NNVEGSPGSSMHGVTGKEQTFAFSV---ASPIVPTDTTAKFALPVDSEHKAKVFKLFSLA 62
           +N EGS G+S+HGVT +EQ F+FSV   +  +   D TAKFALPVDSEH+AKVF   S A
Sbjct: 6   SNEEGSIGTSLHGVTAREQVFSFSVDASSQTVQSDDPTAKFALPVDSEHRAKVFNPLSFA 65

Query: 63  NPHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSRLVM 122
            PHMR FHL W+SFFTCF+STFAAAPLVPIIRDNL+LTK DIGNAGV SVSG+IFSRL M
Sbjct: 66  KPHMRAFHLGWLSFFTCFISTFAAAPLVPIIRDNLDLTKTDIGNAGVASVSGAIFSRLAM 125

Query: 123 GAVCDLLGPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWMSTM 182
           GAVCDLLG RYG AF +ML+APTVF MSFV    GY+ VRFMIGF LATFVSCQYW S M
Sbjct: 126 GAVCDLLGARYGTAFSLMLTAPTVFSMSFVGGPSGYLGVRFMIGFCLATFVSCQYWTSVM 185

Query: 183 FNSQIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLHVIM 242
           FN +IIGLVNG A GWG+MGGG TQL+MP+++ +IK AGATPF AWRIAFF+PG L V+M
Sbjct: 186 FNGKIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLAGATPFMAWRIAFFVPGFLQVVM 245

Query: 243 GILVLTLGQDLPDGNLSSLQKKG--------NVLWYAVTNYRTWIFVLLYGYSMGVELST 302
           GILVL+LGQDLPDGNLS+LQK G         V W+AV NYRTWI  +LYG SMG+EL+ 
Sbjct: 246 GILVLSLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFVLYGSSMGIELTI 305

Query: 303 DNVIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTLWIL 362
           +NVI+ YFYDRF+LKL TAGI+AA+FGMAN +ARPFGGYASD AAR FGMRGRLWTLWI 
Sbjct: 306 NNVISGYFYDRFNLKLQTAGIVAASFGMANFIARPFGGYASDVAARVFGMRGRLWTLWIF 365

Query: 363 QTFGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLTGAG 422
           QT G +FC+WLG+A+ LP AI+AM+LFSIG QAACGA FG+ PF+SRRSLG+ISGLTGAG
Sbjct: 366 QTVGALFCIWLGRASSLPIAILAMMLFSIGTQAACGALFGVAPFVSRRSLGLISGLTGAG 425

Query: 423 GNFGSGLTQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHVDKS 482
           GNFGSGLTQL+FFS++++ST  GL LMG+M V CTLPV  +HFPQWGSMFL P+   ++S
Sbjct: 426 GNFGSGLTQLLFFSSARFSTAEGLSLMGVMAVLCTLPVAFIHFPQWGSMFLRPSTDGERS 485

Query: 483 TEEFYYGSEWTDDEKKKGLHQQSIKFAENSRSERGRRVSSA--PTPPNTTPSHV 524
            EE+YYGSEWT++EK++GLH+ SIKFAENSRSERGR+V+ A  PTP N TPSHV
Sbjct: 486 QEEYYYGSEWTENEKQQGLHEGSIKFAENSRSERGRKVALANIPTPENGTPSHV 539

BLAST of Tan0022574 vs. TAIR 10
Match: AT3G45060.1 (high affinity nitrate transporter 2.6 )

HSP 1 Score: 756.9 bits (1953), Expect = 1.1e-218
Identity = 375/537 (69.83%), Postives = 440/537 (81.94%), Query Frame = 0

Query: 3   NNVEGSPGSSMHGVTGKEQTFAFSV------ASPIVPTDTTAKFALPVDSEHKAKVFKLF 62
           +N +GS G+S+HGVT +EQ F+FSV      +  +   D TAKFALPVDSEH+AKVFK  
Sbjct: 6   SNEDGSIGTSLHGVTAREQVFSFSVQEDVPSSQAVRTNDPTAKFALPVDSEHRAKVFKPL 65

Query: 63  SLANPHMRTFHLSWISFFTCFVSTFAAAPLVPIIRDNLNLTKVDIGNAGVTSVSGSIFSR 122
           S A PHMR FHL WISFFTCF+STFAAAPLVP+IRDNL+LTK DIGNAGV SVSG+IFSR
Sbjct: 66  SFAKPHMRAFHLGWISFFTCFISTFAAAPLVPVIRDNLDLTKTDIGNAGVASVSGAIFSR 125

Query: 123 LVMGAVCDLLGPRYGCAFLIMLSAPTVFCMSFVSSAGGYIAVRFMIGFSLATFVSCQYWM 182
           L MGAVCDLLG RYG AF +ML+AP VF MSFV+ AG Y+AVRFMIGF LATFVSCQYW 
Sbjct: 126 LAMGAVCDLLGARYGTAFSLMLTAPAVFSMSFVADAGSYLAVRFMIGFCLATFVSCQYWT 185

Query: 183 STMFNSQIIGLVNGTAAGWGNMGGGATQLIMPLLYDIIKRAGATPFTAWRIAFFIPGSLH 242
           S MF  +IIGLVNG A GWG+MGGG TQL+MP+++ +IK  GATPFTAWR AFFIPG L 
Sbjct: 186 SVMFTGKIIGLVNGCAGGWGDMGGGVTQLLMPMVFHVIKLTGATPFTAWRFAFFIPGILQ 245

Query: 243 VIMGILVLTLGQDLPDGNLSSLQKKG--------NVLWYAVTNYRTWIFVLLYGYSMGVE 302
           ++MGILVLTLGQDLPDGNLS+LQK G         V W+AV NYRTWI  +LYG+SMGVE
Sbjct: 246 IVMGILVLTLGQDLPDGNLSTLQKSGQVSKDKFSKVFWFAVKNYRTWILFMLYGFSMGVE 305

Query: 303 LSTDNVIAEYFYDRFDLKLHTAGIIAATFGMANLVARPFGGYASDYAARYFGMRGRLWTL 362
           L+ +NVI+ YFYDRF+L LHTAGIIAA+FGMAN  ARPFGGYASD AAR FGMRGRLW L
Sbjct: 306 LTINNVISGYFYDRFNLTLHTAGIIAASFGMANFFARPFGGYASDVAARLFGMRGRLWIL 365

Query: 363 WILQTFGGVFCMWLGKANKLPTAIVAMILFSIGAQAACGATFGIIPFISRRSLGIISGLT 422
           WILQT G +FC+WLG+A+ LP AI+AM+LFS+G QAACGA FG+ PF+SRRSLG+ISGLT
Sbjct: 366 WILQTVGALFCIWLGRASSLPIAILAMMLFSMGTQAACGALFGVAPFVSRRSLGLISGLT 425

Query: 423 GAGGNFGSGLTQLVFFSTSKYSTDTGLFLMGIMIVCCTLPVTLVHFPQWGSMFLPPTKHV 482
           GAGGNFGSG+TQL+FFS+S++ST  GL LMG+M V C+LPV  +HFPQWGSMFL P++  
Sbjct: 426 GAGGNFGSGVTQLLFFSSSRFSTAEGLSLMGVMAVVCSLPVAFIHFPQWGSMFLRPSQDG 485

Query: 483 DKSTEEFYYGSEWTDDEKKKGLHQQSIKFAENSRSERGRR--VSSAPTPPNTTPSHV 524
           +KS EE YYG+EWT++EK  GLH+ SIKFAENSRSERGR+  ++  PTP   +P+HV
Sbjct: 486 EKSKEEHYYGAEWTEEEKSLGLHEGSIKFAENSRSERGRKAMLADIPTPETGSPAHV 542

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FJH86.5e-24579.43High affinity nitrate transporter 2.4 OS=Arabidopsis thaliana OX=3702 GN=NRT2.4 ... [more]
O828119.4e-24479.43High-affinity nitrate transporter 2.1 OS=Arabidopsis thaliana OX=3702 GN=NRT2.1 ... [more]
P0DKG91.5e-23375.14High-affinity nitrate transporter 2.1 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
P0DKH01.5e-23375.14High-affinity nitrate transporter 2.2 OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q9LMZ91.3e-22175.44High-affinity nitrate transporter 2.2 OS=Arabidopsis thaliana OX=3702 GN=NRT2.2 ... [more]
Match NameE-valueIdentityDescription
XP_038895485.14.1e-28995.27high affinity nitrate transporter 2.4-like [Benincasa hispida] >XP_038895611.1 h... [more]
XP_023520099.14.1e-28994.92high affinity nitrate transporter 2.4-like [Cucurbita pepo subsp. pepo] >XP_0235... [more]
XP_022964707.11.6e-28895.09high affinity nitrate transporter 2.4-like [Cucurbita moschata][more]
KAG6583556.14.6e-28894.90High-affinity nitrate transporter 2.1, partial [Cucurbita argyrosperma subsp. so... [more]
XP_022970277.13.9e-28794.52high affinity nitrate transporter 2.4-like [Cucurbita maxima] >XP_022970279.1 hi... [more]
Match NameE-valueIdentityDescription
A0A6J1HIH17.6e-28995.09high affinity nitrate transporter 2.4-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1HYN41.9e-28794.52high affinity nitrate transporter 2.4-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A3Q9CZ424.2e-28794.32High affinity nitrate transporter 2.1 OS=Cucumis sativus OX=3659 GN=NRT2.1 PE=2 ... [more]
S4VM427.1e-28794.13High-affinity nitrate transporter NRT2.2 OS=Cucumis sativus OX=3659 GN=NRT2.2 PE... [more]
Q6PRP81.6e-28694.32High-affinity nitrate transporter OS=Cucumis sativus OX=3659 GN=NRT2 PE=2 SV=3[more]
Match NameE-valueIdentityDescription
AT5G60770.14.6e-24679.43nitrate transporter 2.4 [more]
AT1G08090.16.6e-24579.43nitrate transporter 2:1 [more]
AT1G08100.19.3e-22375.44nitrate transporter 2.2 [more]
AT5G60780.11.0e-22171.16nitrate transporter 2.3 [more]
AT3G45060.11.1e-21869.83high affinity nitrate transporter 2.6 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 522..523
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 484..506
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 507..523
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 484..523
NoneNo IPR availablePANTHERPTHR23515:SF16HIGH-AFFINITY NITRATE TRANSPORTER 2.1-LIKEcoord: 4..520
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 59..254
e-value: 5.6E-21
score: 76.9
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 263..459
e-value: 3.3E-26
score: 93.9
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 60..453
IPR011701Major facilitator superfamilyPFAMPF07690MFS_1coord: 70..418
e-value: 4.6E-18
score: 65.3
IPR044772Nitrate transporterPANTHERPTHR23515HIGH-AFFINITY NITRATE TRANSPORTER 2.3coord: 4..520
IPR044772Nitrate transporterCDDcd17341MFS_NRT2_likecoord: 66..451
e-value: 5.22068E-138
score: 402.015
IPR020846Major facilitator superfamily domainPROSITEPS50850MFScoord: 65..451
score: 8.612638

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0022574.1Tan0022574.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071249 cellular response to nitrate
biological_process GO:0042128 nitrate assimilation
biological_process GO:0015706 nitrate transport
biological_process GO:0031204 posttranslational protein targeting to membrane, translocation
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009705 plant-type vacuole membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0071261 Ssh1 translocon complex
molecular_function GO:0015112 nitrate transmembrane transporter activity
molecular_function GO:0008320 protein transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity