Tan0022569 (gene) Snake gourd v1

Overview
NameTan0022569
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionJ domain-containing protein
LocationLG04: 3484607 .. 3486366 (-)
RNA-Seq ExpressionTan0022569
SyntenyTan0022569
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTGCCGCATGGCTTCCTCTGTACACACCTACTGCTGCAACGAAAATACAGTATCCAACTCAAAGAAAGCTGGGATTGTATAACTCTTCCACTTCCAAGAAGCTTTATGGCAACACTTTGTCATGCAGGGCAGCTTCTTCAATTACAGACTTTGATCTTTATGATCTTCTTGGCATCGACAACACCTCTGATCCATCACGGATTAAGGCAGCCTACCGTGCACTCCAAAAGCAGTGCCATCCCGACATCGCTGGTCCTGCTGGCCACGACATGGCTATCATTCTTAATGAAGTGTATTCGGTTCTTTCAGATCCTAATTCTCGGCTGGCTTACGATAAGGTTAGAACTGAAAACTCAATTCTAATTTGTGCAGCTCTCTGTAGAAACAAGATTGTTTGTGACAAAAGTGGTGTTCGCAGGAGCAAGCAAAAATGGCAGAACTTCGAGGTTACACAGGGAAGCCAGTTTATTCAGTATGGTTGGGATCAGAAAGCGAACAACGGGCGGTTTTTGTAGATGAAGTGAAGTGCATTGGCTGCTTGAAATGTGCTTTATTTGCTGGGAAAACTTTTGCTGTTGAATCGGTGTATGGAAGAGCTAGAGTTGTGGCACAGTGGGCTGATCCTGAATATAAAGTGACGGAGGCCATTGAAGCTTGCCCAGTTGACTGCATTTCGTGAGTGTTTGATCAACCAGTTAAAACTTCTTGTTCAATGTTAAACTTTCCCCTCTCTCTCTCTCTTCCTCTCCTAAGTCCTAATTTGCTTGTAAGTTTCTCTTTCTTGGCAAATCAAACCCAGGATGGTGAAAAGGTCAGATTTGGCAGCTCTAGAGTTTTTAATGTCAAAGCAACCACGTGGAAACGTGAGAGTTGGAATGGGCAACGCAGCTGGTGAACGAGTGTCAAACATTTTCACAGACGTGAAGAAGTTCCAGACTAGATTCAAGGAGGCTACGGAGAAGACTTCAAAACAGCATGTCAAGGTTAGACCCAATGATACCAATGCTAATAGTTCGGCATTGCCGTTATCTTAAAATGACTAAAAAATTGATTAAGTGGGAACCCCAGAAGGTGTGATGTTACCAATTCTTCCACGATAGCGTAATTGAGTTGTACGATTGTTTTTCAGGGGACAACCTTTGAGAGCGAAGCACAGTTGTCTGCAATTCAAGCTATCAGATCAATCTCAAATTGGTTATTTTGGCAGACAGCCACACCAACTGGAACTGGATCAAAACACAGCCAAAACCTGACACACATCGCCAGCAAATCTACACCAGAAATCAACAAACTTCAAGCTGCTGTTACTGCAAGGAAACAACTCAAGGCAAAAACTGAAGACAGAAATGGAGCTACAACAAAATACATAAAGAAGGATGATTACTGGGTTCCGACCACCCTTGCACTCCCAGTCTCAACTCAAGCCCCAAACAGCACAATCTCAAAGCCAAGAGTGGAGACCAAACGTACGAAAGAATCAAAAGACCTTGGTTCTGAAGCTGGTGATGGTGGTCATATTAGCCCCATGAGATTCGCGTTACCAGTGTCAATATCGGTAATTGCAACTGCTGTAATTCAGCAAATGGTGAGAGACAATGGTGCCAACGGCTTGAAGGAACACATCGGAGGTTCTATGGCACTGGAGTTAGTTAACAGTCATTGGATGCAGGTTATACAAACAGGAGTCACTTGGTATGTCATTGGAATGGCAATGATGGGAATGGTCGAAATGATTGCAAGAAAGATTAGGCAGTGA

mRNA sequence

ATGACTGCCGCATGGCTTCCTCTGTACACACCTACTGCTGCAACGAAAATACAGTATCCAACTCAAAGAAAGCTGGGATTGTATAACTCTTCCACTTCCAAGAAGCTTTATGGCAACACTTTGTCATGCAGGGCAGCTTCTTCAATTACAGACTTTGATCTTTATGATCTTCTTGGCATCGACAACACCTCTGATCCATCACGGATTAAGGCAGCCTACCGTGCACTCCAAAAGCAGTGCCATCCCGACATCGCTGGTCCTGCTGGCCACGACATGGCTATCATTCTTAATGAAGTGTATTCGGTTCTTTCAGATCCTAATTCTCGGCTGGCTTACGATAAGGAGCAAGCAAAAATGGCAGAACTTCGAGGTTACACAGGGAAGCCAGTTTATTCAGTATGGTTGGGATCAGAAAGCGAACAACGGGCGGTTTTTGTAGATGAAGTGAAGTGCATTGGCTGCTTGAAATGTGCTTTATTTGCTGGGAAAACTTTTGCTGTTGAATCGGTGTATGGAAGAGCTAGAGTTGTGGCACAGTGGGCTGATCCTGAATATAAAGTGACGGAGGCCATTGAAGCTTGCCCAGTTGACTGCATTTCGATGGTGAAAAGGTCAGATTTGGCAGCTCTAGAGTTTTTAATGTCAAAGCAACCACGTGGAAACGTGAGAGTTGGAATGGGCAACGCAGCTGGTGAACGAGTGTCAAACATTTTCACAGACGTGAAGAAGTTCCAGACTAGATTCAAGGAGGCTACGGAGAAGACTTCAAAACAGCATGTCAAGGGGACAACCTTTGAGAGCGAAGCACAGTTGTCTGCAATTCAAGCTATCAGATCAATCTCAAATTGGTTATTTTGGCAGACAGCCACACCAACTGGAACTGGATCAAAACACAGCCAAAACCTGACACACATCGCCAGCAAATCTACACCAGAAATCAACAAACTTCAAGCTGCTGTTACTGCAAGGAAACAACTCAAGGCAAAAACTGAAGACAGAAATGGAGCTACAACAAAATACATAAAGAAGGATGATTACTGGGTTCCGACCACCCTTGCACTCCCAGTCTCAACTCAAGCCCCAAACAGCACAATCTCAAAGCCAAGAGTGGAGACCAAACGTACGAAAGAATCAAAAGACCTTGGTTCTGAAGCTGGTGATGGTGGTCATATTAGCCCCATGAGATTCGCGTTACCAGTGTCAATATCGGTAATTGCAACTGCTGTAATTCAGCAAATGGTGAGAGACAATGGTGCCAACGGCTTGAAGGAACACATCGGAGGTTCTATGGCACTGGAGTTAGTTAACAGTCATTGGATGCAGGTTATACAAACAGGAGTCACTTGGTATGTCATTGGAATGGCAATGATGGGAATGGTCGAAATGATTGCAAGAAAGATTAGGCAGTGA

Coding sequence (CDS)

ATGACTGCCGCATGGCTTCCTCTGTACACACCTACTGCTGCAACGAAAATACAGTATCCAACTCAAAGAAAGCTGGGATTGTATAACTCTTCCACTTCCAAGAAGCTTTATGGCAACACTTTGTCATGCAGGGCAGCTTCTTCAATTACAGACTTTGATCTTTATGATCTTCTTGGCATCGACAACACCTCTGATCCATCACGGATTAAGGCAGCCTACCGTGCACTCCAAAAGCAGTGCCATCCCGACATCGCTGGTCCTGCTGGCCACGACATGGCTATCATTCTTAATGAAGTGTATTCGGTTCTTTCAGATCCTAATTCTCGGCTGGCTTACGATAAGGAGCAAGCAAAAATGGCAGAACTTCGAGGTTACACAGGGAAGCCAGTTTATTCAGTATGGTTGGGATCAGAAAGCGAACAACGGGCGGTTTTTGTAGATGAAGTGAAGTGCATTGGCTGCTTGAAATGTGCTTTATTTGCTGGGAAAACTTTTGCTGTTGAATCGGTGTATGGAAGAGCTAGAGTTGTGGCACAGTGGGCTGATCCTGAATATAAAGTGACGGAGGCCATTGAAGCTTGCCCAGTTGACTGCATTTCGATGGTGAAAAGGTCAGATTTGGCAGCTCTAGAGTTTTTAATGTCAAAGCAACCACGTGGAAACGTGAGAGTTGGAATGGGCAACGCAGCTGGTGAACGAGTGTCAAACATTTTCACAGACGTGAAGAAGTTCCAGACTAGATTCAAGGAGGCTACGGAGAAGACTTCAAAACAGCATGTCAAGGGGACAACCTTTGAGAGCGAAGCACAGTTGTCTGCAATTCAAGCTATCAGATCAATCTCAAATTGGTTATTTTGGCAGACAGCCACACCAACTGGAACTGGATCAAAACACAGCCAAAACCTGACACACATCGCCAGCAAATCTACACCAGAAATCAACAAACTTCAAGCTGCTGTTACTGCAAGGAAACAACTCAAGGCAAAAACTGAAGACAGAAATGGAGCTACAACAAAATACATAAAGAAGGATGATTACTGGGTTCCGACCACCCTTGCACTCCCAGTCTCAACTCAAGCCCCAAACAGCACAATCTCAAAGCCAAGAGTGGAGACCAAACGTACGAAAGAATCAAAAGACCTTGGTTCTGAAGCTGGTGATGGTGGTCATATTAGCCCCATGAGATTCGCGTTACCAGTGTCAATATCGGTAATTGCAACTGCTGTAATTCAGCAAATGGTGAGAGACAATGGTGCCAACGGCTTGAAGGAACACATCGGAGGTTCTATGGCACTGGAGTTAGTTAACAGTCATTGGATGCAGGTTATACAAACAGGAGTCACTTGGTATGTCATTGGAATGGCAATGATGGGAATGGTCGAAATGATTGCAAGAAAGATTAGGCAGTGA

Protein sequence

MTAAWLPLYTPTAATKIQYPTQRKLGLYNSSTSKKLYGNTLSCRAASSITDFDLYDLLGIDNTSDPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMAELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPEYKVTEAIEACPVDCISMVKRSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTDVKKFQTRFKEATEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPTGTGSKHSQNLTHIASKSTPEINKLQAAVTARKQLKAKTEDRNGATTKYIKKDDYWVPTTLALPVSTQAPNSTISKPRVETKRTKESKDLGSEAGDGGHISPMRFALPVSISVIATAVIQQMVRDNGANGLKEHIGGSMALELVNSHWMQVIQTGVTWYVIGMAMMGMVEMIARKIRQ
Homology
BLAST of Tan0022569 vs. ExPASy Swiss-Prot
Match: Q9FMX6 (Chaperone protein dnaJ C76, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DJC76 PE=2 SV=1)

HSP 1 Score: 455.3 bits (1170), Expect = 8.4e-127
Identity = 245/449 (54.57%), Postives = 309/449 (68.82%), Query Frame = 0

Query: 31  STSKKLY----GNTLSCRA---ASSITDFDLYDLLGIDNTSDPSRIKAAYRALQKQCHPD 90
           STS+KL          CRA   +SSITDFDLYDLLGID +SD S+IK+AYRALQK+CHPD
Sbjct: 21  STSQKLITVRSPLKFKCRATSSSSSITDFDLYDLLGIDRSSDKSQIKSAYRALQKRCHPD 80

Query: 91  IAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMAELRGYTGKPVYSVWLGSESEQRA 150
           IAG  GHDMAIILNE Y +LSDP SR AYDKEQAK+ ELRGYTGKP+YSVW G E+EQRA
Sbjct: 81  IAGDPGHDMAIILNEAYQLLSDPISRQAYDKEQAKLEELRGYTGKPIYSVWCGPETEQRA 140

Query: 151 VFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPEYKVTEAIEACPVDCISMVK 210
            FVDEVKC+GCLKCAL A KTFA+E+ YGRARVVAQWADPE K+ EAIEACPVDCISMV+
Sbjct: 141 AFVDEVKCVGCLKCALCAEKTFAIETAYGRARVVAQWADPESKIKEAIEACPVDCISMVE 200

Query: 211 RSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTDVKKFQTRFKEATEKTSKQHVKGT 270
           RSDLA LEFLMSKQPRGNVR+G+GN  GERVSN+F DVKKFQ R+ +A  +T+K+     
Sbjct: 201 RSDLAPLEFLMSKQPRGNVRIGVGNTVGERVSNVFVDVKKFQERYAKAMSRTTKE----- 260

Query: 271 TFESEAQLSAIQAIRSISNWLFWQTA---TPTGTGSKHSQNLTHIASKSTPEINKLQAAV 330
           T + E Q+SA++AIRSISNWL+W+++    P    S  S   T       P+I KLQ  V
Sbjct: 261 TSQREVQISAVEAIRSISNWLYWRSSPYTKPLSPESNMSLTFTKRKKAVDPDIRKLQDVV 320

Query: 331 TARKQL--KAKTEDRNGATTKYIKKDDYWVPTTLALPVSTQAPNSTISKPRVETKRTKES 390
            A KQ     +T+++  A   Y+  +DYW P+  ALP S     S  S     T++T  S
Sbjct: 321 AAMKQADQSGRTKEKGSA---YLLGEDYWSPSNAALPSSGNNNGSKASSNPQVTRKTFPS 380

Query: 391 KDLGSEAGDGGHISPMRFALPVSISVIATAVIQQMVRDNGANGLKEHIGGSMALELVNSH 450
           ++  +   +      ++   P+  +++A  ++Q       A+ L +HIGGS+AL +VNS 
Sbjct: 381 EEKPTSRRENRRQFRIK-KFPIGTAIVAVFLVQYQASYRAASELNDHIGGSLALSIVNSP 440

Query: 451 WMQVIQTGVTWYVIGMAMMGMVEMIARKI 468
           W Q++  GVTWY IG  ++ +VE +  K+
Sbjct: 441 WQQILLAGVTWYFIGAMLLQLVEAVQHKL 460

BLAST of Tan0022569 vs. ExPASy Swiss-Prot
Match: Q8G6C6 (Chaperone protein DnaJ OS=Bifidobacterium longum (strain NCC 2705) OX=206672 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 7.9e-08
Identity = 27/61 (44.26%), Postives = 36/61 (59.02%), Query Frame = 0

Query: 53  DLYDLLGIDNTSDPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAY 112
           D Y+ LG++  +    IK AYR L ++ HPDIAGP   D    +N  Y VLS+P+ R  Y
Sbjct: 3   DYYETLGVERGASDDEIKKAYRKLSRKYHPDIAGPEFEDKFKEVNNAYDVLSNPDKRRMY 62

Query: 113 D 114
           D
Sbjct: 63  D 63

BLAST of Tan0022569 vs. ExPASy Swiss-Prot
Match: B5YAR4 (Chaperone protein DnaJ OS=Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) OX=309799 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.0e-07
Identity = 28/66 (42.42%), Postives = 38/66 (57.58%), Query Frame = 0

Query: 50  TDFDLYDLLGIDNTSDPSRIKAAYRALQKQCHPDI-AGPAGHDMAIILNEVYSVLSDPNS 109
           T  D Y++LG+   +    IK AYR L +Q HPD+   P+ H+    +NE Y VLSDP  
Sbjct: 3   TKKDYYEILGVPRNATQDEIKQAYRRLVRQYHPDLNKDPSAHEKFKEINEAYEVLSDPQK 62

Query: 110 RLAYDK 115
           R  YD+
Sbjct: 63  RAQYDQ 68

BLAST of Tan0022569 vs. ExPASy Swiss-Prot
Match: Q7V9C8 (Chaperone protein DnaJ OS=Prochlorococcus marinus (strain MIT 9313) OX=74547 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 1.3e-07
Identity = 34/78 (43.59%), Postives = 40/78 (51.28%), Query Frame = 0

Query: 53  DLYDLLGIDNTSDPSRIKAAYRALQKQCHPDI-AGPAGHDMAIILNEVYSVLSDPNSRLA 112
           D YDLLG+   +D   +K AYR L +Q HPDI   P   D    +   Y VLSDP +R  
Sbjct: 3   DYYDLLGVSKDADGDTLKRAYRRLARQYHPDINKDPGAEDRFKEIGRAYEVLSDPQTRGR 62

Query: 113 YDKEQAKMAELRGYTGKP 130
           YD  Q   A L G  G P
Sbjct: 63  YD--QFGEAGLGGAAGMP 78

BLAST of Tan0022569 vs. ExPASy Swiss-Prot
Match: Q0AKB3 (Chaperone protein DnaJ OS=Maricaulis maris (strain MCS10) OX=394221 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 2.3e-07
Identity = 29/64 (45.31%), Postives = 40/64 (62.50%), Query Frame = 0

Query: 53  DLYDLLGIDNTSDPSRIKAAYRALQKQCHPD-IAGPAGHDMAI-ILNEVYSVLSDPNSRL 112
           D Y++LG+D T+D   +K+AYR    + HPD   G A  +    ++ E YSVLSDPN R 
Sbjct: 5   DFYEVLGVDKTADEKTLKSAYRKQAMKYHPDRNPGDAEAEAQFKVVGEAYSVLSDPNKRA 64

Query: 113 AYDK 115
           AYD+
Sbjct: 65  AYDR 68

BLAST of Tan0022569 vs. NCBI nr
Match: KAG6600118.1 (Chaperone protein dnaJ C76, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 806.6 bits (2082), Expect = 1.1e-229
Identity = 415/468 (88.68%), Postives = 434/468 (92.74%), Query Frame = 0

Query: 1   MTAAWLPLYTPTAATKIQYPTQRKLGLYNSSTSKKLYGNTLSCRAASSITDFDLYDLLGI 60
           M AAWLPLYTP  ATKIQYPTQRKLG YNSSTSK LYGNTL+CRA SSITDFDLYDLLGI
Sbjct: 1   MNAAWLPLYTPIVATKIQYPTQRKLGSYNSSTSKMLYGNTLACRAGSSITDFDLYDLLGI 60

Query: 61  DNTSDPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120
           DNTSD SRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA
Sbjct: 61  DNTSDSSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120

Query: 121 ELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180
           ELRGYTGK VYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW
Sbjct: 121 ELRGYTGKSVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180

Query: 181 ADPEYKVTEAIEACPVDCISMVKRSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240
           ADPEYKV EAIEACPVDCISMV+RSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD
Sbjct: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240

Query: 241 VKKFQTRFKEATEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPTGTGSKHSQ 300
           VKKFQTRFKEA EKTSKQHVKGTTFE++AQLSAIQAIRSISN LFWQTAT     SKHSQ
Sbjct: 241 VKKFQTRFKEAMEKTSKQHVKGTTFENKAQLSAIQAIRSISNCLFWQTATV----SKHSQ 300

Query: 301 NLTHIASKSTPEINKLQAAVTARKQLKAKTEDRNGATTKYIKKDDYWVPTTLALPVSTQA 360
           NLT + +KSTP+INKLQAA+TARKQLKAKTED+NG TTKYI KDDYWVPTTLALPVSTQ 
Sbjct: 301 NLTLVVNKSTPQINKLQAAITARKQLKAKTEDKNGTTTKYI-KDDYWVPTTLALPVSTQT 360

Query: 361 PNSTISKPRVETKRTKESKDLGSEAGDGGHISPMRFALPVSISVIATAVIQQMVRDNGAN 420
           P STIS PRVETKR+KESKDL  E G G H SPMR ALPV IS++AT VIQQMV D+GA+
Sbjct: 361 PISTISNPRVETKRSKESKDLDFEVGGGDHTSPMRLALPVLISIVATTVIQQMVTDDGAS 420

Query: 421 GLKEHIGGSMALELVNSHWMQVIQTGVTWYVIGMAMMGMVEMIARKIR 469
           GLKEHIGGS+ALELVNSHWMQ+IQ GVTWYVIG+A++GMVEMIARKIR
Sbjct: 421 GLKEHIGGSVALELVNSHWMQLIQRGVTWYVIGIAVIGMVEMIARKIR 463

BLAST of Tan0022569 vs. NCBI nr
Match: XP_022942611.1 (chaperone protein dnaJ C76, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 802.4 bits (2071), Expect = 2.1e-228
Identity = 413/468 (88.25%), Postives = 431/468 (92.09%), Query Frame = 0

Query: 1   MTAAWLPLYTPTAATKIQYPTQRKLGLYNSSTSKKLYGNTLSCRAASSITDFDLYDLLGI 60
           M AAWLPLYTP  ATKIQYPTQRKLG Y SSTSK LYGNTL+CRA SSITDFDLYDLLGI
Sbjct: 1   MNAAWLPLYTPIVATKIQYPTQRKLGSYYSSTSKMLYGNTLACRAGSSITDFDLYDLLGI 60

Query: 61  DNTSDPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120
           DNTSD SRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA
Sbjct: 61  DNTSDSSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120

Query: 121 ELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180
           ELRGYTGK VYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW
Sbjct: 121 ELRGYTGKSVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180

Query: 181 ADPEYKVTEAIEACPVDCISMVKRSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240
           ADPEYKV EAIEACPVDCISMV+RSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD
Sbjct: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240

Query: 241 VKKFQTRFKEATEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPTGTGSKHSQ 300
           VKKFQTRFKEA EKTSKQHVKGTTFE++AQLSAIQAIRSISN LFWQTA    TGSKHSQ
Sbjct: 241 VKKFQTRFKEAMEKTSKQHVKGTTFENKAQLSAIQAIRSISNCLFWQTA----TGSKHSQ 300

Query: 301 NLTHIASKSTPEINKLQAAVTARKQLKAKTEDRNGATTKYIKKDDYWVPTTLALPVSTQA 360
           NLT + +KSTP+INKLQAA+TARKQLKAKTEDRNG TTKYI  DDYWVPTTLALPVSTQ 
Sbjct: 301 NLTLVVNKSTPQINKLQAAITARKQLKAKTEDRNGTTTKYI-TDDYWVPTTLALPVSTQT 360

Query: 361 PNSTISKPRVETKRTKESKDLGSEAGDGGHISPMRFALPVSISVIATAVIQQMVRDNGAN 420
           P STIS PRVETKR+KESKDL  E G G H SPMR ALPV IS++AT VIQQMV D+GA+
Sbjct: 361 PISTISNPRVETKRSKESKDLDFEVGGGDHTSPMRLALPVLISIVATTVIQQMVTDDGAS 420

Query: 421 GLKEHIGGSMALELVNSHWMQVIQTGVTWYVIGMAMMGMVEMIARKIR 469
           GLKEHIGGS+ALE VNSHWMQ+IQ GVTWYVIG+A++GMVEMI RKIR
Sbjct: 421 GLKEHIGGSVALEFVNSHWMQLIQRGVTWYVIGIAVIGMVEMIGRKIR 463

BLAST of Tan0022569 vs. NCBI nr
Match: XP_023532693.1 (chaperone protein dnaJ C76, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 798.5 bits (2061), Expect = 3.1e-227
Identity = 411/468 (87.82%), Postives = 432/468 (92.31%), Query Frame = 0

Query: 1   MTAAWLPLYTPTAATKIQYPTQRKLGLYNSSTSKKLYGNTLSCRAASSITDFDLYDLLGI 60
           MTAAWLPLYTP  ATKIQ PT+RKLG YN STSK LYGNTL+CRA SSITDFDLYDLLGI
Sbjct: 38  MTAAWLPLYTPVVATKIQNPTRRKLGSYNFSTSKMLYGNTLACRAGSSITDFDLYDLLGI 97

Query: 61  DNTSDPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120
           DNTSD SRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA
Sbjct: 98  DNTSDSSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 157

Query: 121 ELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180
           ELRGYTGK VYSVWLGSESE+RAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW
Sbjct: 158 ELRGYTGKSVYSVWLGSESERRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 217

Query: 181 ADPEYKVTEAIEACPVDCISMVKRSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240
           ADPEYKV EAIEACPVDCISMV+RSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD
Sbjct: 218 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 277

Query: 241 VKKFQTRFKEATEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPTGTGSKHSQ 300
           VKKFQTRFKEA EKTSKQHVKGTTFE++AQLSAIQAIRSISN LFWQTA    TGSKHSQ
Sbjct: 278 VKKFQTRFKEAMEKTSKQHVKGTTFENKAQLSAIQAIRSISNCLFWQTA----TGSKHSQ 337

Query: 301 NLTHIASKSTPEINKLQAAVTARKQLKAKTEDRNGATTKYIKKDDYWVPTTLALPVSTQA 360
           NLT + + STP+INKLQAA+TARKQLKAKTED+NG TTKYI KDDYWVPTTLALPVSTQ 
Sbjct: 338 NLTLVVNTSTPQINKLQAAITARKQLKAKTEDKNGTTTKYI-KDDYWVPTTLALPVSTQT 397

Query: 361 PNSTISKPRVETKRTKESKDLGSEAGDGGHISPMRFALPVSISVIATAVIQQMVRDNGAN 420
           P STIS PRVETKR+KESKDL  E G G H SPMR ALPV IS++AT VIQQMV D+GA+
Sbjct: 398 PISTISNPRVETKRSKESKDLDFEVGGGDHTSPMRLALPVLISIVATTVIQQMVTDDGAS 457

Query: 421 GLKEHIGGSMALELVNSHWMQVIQTGVTWYVIGMAMMGMVEMIARKIR 469
           GLKEHIGGS+ALELVNSHWMQ+IQ GVTWYVIG+A++GMVE IARKIR
Sbjct: 458 GLKEHIGGSVALELVNSHWMQLIQRGVTWYVIGIAVIGMVEKIARKIR 500

BLAST of Tan0022569 vs. NCBI nr
Match: XP_022987962.1 (chaperone protein dnaJ C76, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 786.2 bits (2029), Expect = 1.6e-223
Identity = 406/468 (86.75%), Postives = 427/468 (91.24%), Query Frame = 0

Query: 1   MTAAWLPLYTPTAATKIQYPTQRKLGLYNSSTSKKLYGNTLSCRAASSITDFDLYDLLGI 60
           MTAAWLPLYTP  ATKIQ PT+RKLG YN S SK LYGNTL+CRA SSITDFDLYDLLGI
Sbjct: 1   MTAAWLPLYTPIVATKIQNPTRRKLGSYNFSFSKMLYGNTLACRAGSSITDFDLYDLLGI 60

Query: 61  DNTSDPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120
           DNTSD SRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA
Sbjct: 61  DNTSDSSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120

Query: 121 ELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180
           ELRGYTGK VYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW
Sbjct: 121 ELRGYTGKSVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180

Query: 181 ADPEYKVTEAIEACPVDCISMVKRSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240
           ADPEYKV EAIEACPVDCISMV+RSDLAALEFLMSKQPRGNVRVGMGNAA ERVSNIFTD
Sbjct: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAASERVSNIFTD 240

Query: 241 VKKFQTRFKEATEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPTGTGSKHSQ 300
           VKKFQ RFKEA EKTS QHVKGTTFE++AQLSAIQAIRSISN LFWQTA    TG+KHSQ
Sbjct: 241 VKKFQIRFKEAMEKTSNQHVKGTTFENKAQLSAIQAIRSISNCLFWQTA----TGTKHSQ 300

Query: 301 NLTHIASKSTPEINKLQAAVTARKQLKAKTEDRNGATTKYIKKDDYWVPTTLALPVSTQA 360
           NL  + +KSTP+INKLQAA+TARKQLKAKTED+N  TTKYI KDDYWVPTTLALPVSTQ 
Sbjct: 301 NLMLVVNKSTPQINKLQAAITARKQLKAKTEDKNETTTKYI-KDDYWVPTTLALPVSTQT 360

Query: 361 PNSTISKPRVETKRTKESKDLGSEAGDGGHISPMRFALPVSISVIATAVIQQMVRDNGAN 420
           P STIS PRVETKR+KESKDL  E G G H SPMR ALPV IS++AT VIQQMV D+GA+
Sbjct: 361 PISTISNPRVETKRSKESKDLDFEVGGGDHTSPMRLALPVLISIVATNVIQQMVTDDGAS 420

Query: 421 GLKEHIGGSMALELVNSHWMQVIQTGVTWYVIGMAMMGMVEMIARKIR 469
           G KEHIGGS+ALELVNSHWMQ+IQ GVTWYVIG+A++GMVEMIARKIR
Sbjct: 421 GFKEHIGGSVALELVNSHWMQLIQRGVTWYVIGIAVIGMVEMIARKIR 463

BLAST of Tan0022569 vs. NCBI nr
Match: XP_023543201.1 (chaperone protein dnaJ C76, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 780.8 bits (2015), Expect = 6.7e-222
Identity = 396/469 (84.43%), Postives = 425/469 (90.62%), Query Frame = 0

Query: 1   MTAAWLPLYTPT-AATKIQYPTQRKLGLYNSSTSKKLYGNTLSCRAASSITDFDLYDLLG 60
           M+ +WLPLYT T AAT  QYPTQ KLG YNSSTS+K YGNTLSCRAASSITDFDLYDLLG
Sbjct: 1   MSTSWLPLYTHTAAATARQYPTQTKLGSYNSSTSRKPYGNTLSCRAASSITDFDLYDLLG 60

Query: 61  IDNTSDPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKM 120
           ID++SD SRIKAAYRALQKQCHPDIAGPAGHDM+IILNE YS+LSDP+SRLAYDKEQAKM
Sbjct: 61  IDSSSDSSRIKAAYRALQKQCHPDIAGPAGHDMSIILNEAYSLLSDPSSRLAYDKEQAKM 120

Query: 121 AELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ 180
           AELRGYTG PVYSVWLGSE+EQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ
Sbjct: 121 AELRGYTGNPVYSVWLGSENEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ 180

Query: 181 WADPEYKVTEAIEACPVDCISMVKRSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFT 240
           WADPEYKV EAI+ CPVDCISMV+RSDLAALEFLMSKQPRGNVRVGM NAAGERVSNIFT
Sbjct: 181 WADPEYKVMEAIQTCPVDCISMVERSDLAALEFLMSKQPRGNVRVGMSNAAGERVSNIFT 240

Query: 241 DVKKFQTRFKEATEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPTGTGSKHS 300
           DVKKFQ RFKEA EK SK+H KG TFE+EAQL+AIQ IRSISNWLFWQTATP G GSK  
Sbjct: 241 DVKKFQARFKEAMEKASKEHSKGPTFENEAQLAAIQTIRSISNWLFWQTATPAGPGSKQG 300

Query: 301 QNLTHIASKSTPEINKLQAAVTARKQLKAKTEDRNGATTKYIKKDDYWVPTTLALPVSTQ 360
           QNLTHIA+KSTPEIN+LQAAVTARKQ++AK EDRN AT KYI KDDYWVPTTLALP STQ
Sbjct: 301 QNLTHIATKSTPEINRLQAAVTARKQIRAKAEDRNRATAKYIHKDDYWVPTTLALPTSTQ 360

Query: 361 APNSTISKPRVETKRTKESKDLGSEAGDGGHISPMRFALPVSISVIATAVIQQMVRDNGA 420
            PNSTISK RVETK TKE + L    GDGGH+SPMR A+PVSIS+IATA IQQM+R++GA
Sbjct: 361 TPNSTISKSRVETKPTKEPRGL----GDGGHVSPMRLAIPVSISIIATAAIQQMMRNDGA 420

Query: 421 NGLKEHIGGSMALELVNSHWMQVIQTGVTWYVIGMAMMGMVEMIARKIR 469
           + LKEHIGGSMALELVNSHWMQV+ TGVTWY+IGM +MGMVEMI RKIR
Sbjct: 421 SSLKEHIGGSMALELVNSHWMQVMLTGVTWYIIGMVVMGMVEMIGRKIR 465

BLAST of Tan0022569 vs. ExPASy TrEMBL
Match: A0A6J1FPC3 (chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111447595 PE=4 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 1.0e-228
Identity = 413/468 (88.25%), Postives = 431/468 (92.09%), Query Frame = 0

Query: 1   MTAAWLPLYTPTAATKIQYPTQRKLGLYNSSTSKKLYGNTLSCRAASSITDFDLYDLLGI 60
           M AAWLPLYTP  ATKIQYPTQRKLG Y SSTSK LYGNTL+CRA SSITDFDLYDLLGI
Sbjct: 1   MNAAWLPLYTPIVATKIQYPTQRKLGSYYSSTSKMLYGNTLACRAGSSITDFDLYDLLGI 60

Query: 61  DNTSDPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120
           DNTSD SRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA
Sbjct: 61  DNTSDSSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120

Query: 121 ELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180
           ELRGYTGK VYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW
Sbjct: 121 ELRGYTGKSVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180

Query: 181 ADPEYKVTEAIEACPVDCISMVKRSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240
           ADPEYKV EAIEACPVDCISMV+RSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD
Sbjct: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240

Query: 241 VKKFQTRFKEATEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPTGTGSKHSQ 300
           VKKFQTRFKEA EKTSKQHVKGTTFE++AQLSAIQAIRSISN LFWQTA    TGSKHSQ
Sbjct: 241 VKKFQTRFKEAMEKTSKQHVKGTTFENKAQLSAIQAIRSISNCLFWQTA----TGSKHSQ 300

Query: 301 NLTHIASKSTPEINKLQAAVTARKQLKAKTEDRNGATTKYIKKDDYWVPTTLALPVSTQA 360
           NLT + +KSTP+INKLQAA+TARKQLKAKTEDRNG TTKYI  DDYWVPTTLALPVSTQ 
Sbjct: 301 NLTLVVNKSTPQINKLQAAITARKQLKAKTEDRNGTTTKYI-TDDYWVPTTLALPVSTQT 360

Query: 361 PNSTISKPRVETKRTKESKDLGSEAGDGGHISPMRFALPVSISVIATAVIQQMVRDNGAN 420
           P STIS PRVETKR+KESKDL  E G G H SPMR ALPV IS++AT VIQQMV D+GA+
Sbjct: 361 PISTISNPRVETKRSKESKDLDFEVGGGDHTSPMRLALPVLISIVATTVIQQMVTDDGAS 420

Query: 421 GLKEHIGGSMALELVNSHWMQVIQTGVTWYVIGMAMMGMVEMIARKIR 469
           GLKEHIGGS+ALE VNSHWMQ+IQ GVTWYVIG+A++GMVEMI RKIR
Sbjct: 421 GLKEHIGGSVALEFVNSHWMQLIQRGVTWYVIGIAVIGMVEMIGRKIR 463

BLAST of Tan0022569 vs. ExPASy TrEMBL
Match: A0A6J1JBT2 (chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111485354 PE=4 SV=1)

HSP 1 Score: 786.2 bits (2029), Expect = 7.7e-224
Identity = 406/468 (86.75%), Postives = 427/468 (91.24%), Query Frame = 0

Query: 1   MTAAWLPLYTPTAATKIQYPTQRKLGLYNSSTSKKLYGNTLSCRAASSITDFDLYDLLGI 60
           MTAAWLPLYTP  ATKIQ PT+RKLG YN S SK LYGNTL+CRA SSITDFDLYDLLGI
Sbjct: 1   MTAAWLPLYTPIVATKIQNPTRRKLGSYNFSFSKMLYGNTLACRAGSSITDFDLYDLLGI 60

Query: 61  DNTSDPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120
           DNTSD SRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA
Sbjct: 61  DNTSDSSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120

Query: 121 ELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180
           ELRGYTGK VYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW
Sbjct: 121 ELRGYTGKSVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180

Query: 181 ADPEYKVTEAIEACPVDCISMVKRSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240
           ADPEYKV EAIEACPVDCISMV+RSDLAALEFLMSKQPRGNVRVGMGNAA ERVSNIFTD
Sbjct: 181 ADPEYKVMEAIEACPVDCISMVERSDLAALEFLMSKQPRGNVRVGMGNAASERVSNIFTD 240

Query: 241 VKKFQTRFKEATEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPTGTGSKHSQ 300
           VKKFQ RFKEA EKTS QHVKGTTFE++AQLSAIQAIRSISN LFWQTA    TG+KHSQ
Sbjct: 241 VKKFQIRFKEAMEKTSNQHVKGTTFENKAQLSAIQAIRSISNCLFWQTA----TGTKHSQ 300

Query: 301 NLTHIASKSTPEINKLQAAVTARKQLKAKTEDRNGATTKYIKKDDYWVPTTLALPVSTQA 360
           NL  + +KSTP+INKLQAA+TARKQLKAKTED+N  TTKYI KDDYWVPTTLALPVSTQ 
Sbjct: 301 NLMLVVNKSTPQINKLQAAITARKQLKAKTEDKNETTTKYI-KDDYWVPTTLALPVSTQT 360

Query: 361 PNSTISKPRVETKRTKESKDLGSEAGDGGHISPMRFALPVSISVIATAVIQQMVRDNGAN 420
           P STIS PRVETKR+KESKDL  E G G H SPMR ALPV IS++AT VIQQMV D+GA+
Sbjct: 361 PISTISNPRVETKRSKESKDLDFEVGGGDHTSPMRLALPVLISIVATNVIQQMVTDDGAS 420

Query: 421 GLKEHIGGSMALELVNSHWMQVIQTGVTWYVIGMAMMGMVEMIARKIR 469
           G KEHIGGS+ALELVNSHWMQ+IQ GVTWYVIG+A++GMVEMIARKIR
Sbjct: 421 GFKEHIGGSVALELVNSHWMQLIQRGVTWYVIGIAVIGMVEMIARKIR 463

BLAST of Tan0022569 vs. ExPASy TrEMBL
Match: A0A6J1GZ80 (chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111458571 PE=4 SV=1)

HSP 1 Score: 776.5 bits (2004), Expect = 6.1e-221
Identity = 394/469 (84.01%), Postives = 424/469 (90.41%), Query Frame = 0

Query: 1   MTAAWLPLYTPT-AATKIQYPTQRKLGLYNSSTSKKLYGNTLSCRAASSITDFDLYDLLG 60
           M+ +WLPLYT T AAT  QYPTQ KLG YNSSTS+K YGNTLSCRAASSITDFDLYDLLG
Sbjct: 1   MSTSWLPLYTHTAAATARQYPTQTKLGSYNSSTSRKPYGNTLSCRAASSITDFDLYDLLG 60

Query: 61  IDNTSDPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKM 120
           ID++SD SRIKAAYRALQKQCHPDIAGPAGHDM+IILNE YS+LSDP+SRLAYDKEQAKM
Sbjct: 61  IDSSSDSSRIKAAYRALQKQCHPDIAGPAGHDMSIILNEAYSLLSDPSSRLAYDKEQAKM 120

Query: 121 AELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ 180
           AELRGYTG PVYSVWLGSE+EQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ
Sbjct: 121 AELRGYTGNPVYSVWLGSENEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQ 180

Query: 181 WADPEYKVTEAIEACPVDCISMVKRSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFT 240
           WADPEYKV EAI+ CPVDCISMV+RSDLAALEFLMSKQPRGNVRVGM NAAGERVSNIFT
Sbjct: 181 WADPEYKVMEAIQTCPVDCISMVERSDLAALEFLMSKQPRGNVRVGMSNAAGERVSNIFT 240

Query: 241 DVKKFQTRFKEATEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPTGTGSKHS 300
           DVKKFQ RFKEA EK SK+H KG TFE+EAQL+AIQ IRSISNWLFWQTATP G GSK  
Sbjct: 241 DVKKFQARFKEAMEKASKEHSKGATFENEAQLAAIQTIRSISNWLFWQTATPAGPGSKQG 300

Query: 301 QNLTHIASKSTPEINKLQAAVTARKQLKAKTEDRNGATTKYIKKDDYWVPTTLALPVSTQ 360
           QNLTHIA+KSTPEIN+LQAAVTARKQ++AK EDRN AT K+I KDDYWVPTTLALP STQ
Sbjct: 301 QNLTHIATKSTPEINRLQAAVTARKQIRAKAEDRNRATEKHIYKDDYWVPTTLALPTSTQ 360

Query: 361 APNSTISKPRVETKRTKESKDLGSEAGDGGHISPMRFALPVSISVIATAVIQQMVRDNGA 420
            PNSTISK RVETK  KE + L    GDGGH+SPMR ALP+SIS+IATA IQQM+R++GA
Sbjct: 361 TPNSTISKSRVETKPIKEPRGL----GDGGHVSPMRLALPISISIIATAAIQQMMRNDGA 420

Query: 421 NGLKEHIGGSMALELVNSHWMQVIQTGVTWYVIGMAMMGMVEMIARKIR 469
           + LKEHIGGSMALELVNSHWMQV+ TGVTWY+IGM +MGMVEMI RKIR
Sbjct: 421 SSLKEHIGGSMALELVNSHWMQVMLTGVTWYIIGMVVMGMVEMIGRKIR 465

BLAST of Tan0022569 vs. ExPASy TrEMBL
Match: A0A0A0KPY4 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G175710 PE=4 SV=1)

HSP 1 Score: 770.0 bits (1987), Expect = 5.7e-219
Identity = 388/468 (82.91%), Postives = 418/468 (89.32%), Query Frame = 0

Query: 1   MTAAWLPLYTPTAATKIQYPTQRKLGLYNSSTSKKLYGNTLSCRAASSITDFDLYDLLGI 60
           M+A+WLPLYTPTA    QYP QRKLG YN STS+KL+GNTLSC+AASSITDFDLYDLLGI
Sbjct: 1   MSASWLPLYTPTA----QYPIQRKLGSYNPSTSRKLHGNTLSCKAASSITDFDLYDLLGI 60

Query: 61  DNTSDPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120
           DNTS PSRIKAAYRALQK CHPDIAGPAGHDMAIILNE YSVLSDP+SRLAYDKEQAKMA
Sbjct: 61  DNTSHPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMA 120

Query: 121 ELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180
           ELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW
Sbjct: 121 ELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180

Query: 181 ADPEYKVTEAIEACPVDCISMVKRSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240
           ADPEYKV EAIEACPVDCISMV+R+DLAALEFLMSKQPRGNVRVGMGN AGERVSNIFTD
Sbjct: 181 ADPEYKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTD 240

Query: 241 VKKFQTRFKEATEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPTGTGSKHSQ 300
           VKKFQ +F EA EK  K+  KG TFESE QL+AIQAIRSISNWLFWQTATP G GSK SQ
Sbjct: 241 VKKFQIKFNEAMEKAMKEQSKGATFESEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQ 300

Query: 301 NLTHIASKSTPEINKLQAAVTARKQLKAKTEDRNGATTKYIKKDDYWVPTTLALPVSTQA 360
           +L   ASK TPEINKLQAA TARKQ++ K EDRN  TTKY+ +DDYWVPTT ALP STQ+
Sbjct: 301 SLARSASKFTPEINKLQAAATARKQIREKAEDRNRTTTKYLYRDDYWVPTTFALPASTQS 360

Query: 361 PNSTISKPRVETKRTKESKDLGSEAGDGGHISPMRFALPVSISVIATAVIQQMVRDNGAN 420
           PN+ ISKP VETK TK+S+ LGS+   GGH+SPMR  LPVSIS+IATA+IQQMVR++GA+
Sbjct: 361 PNNPISKPSVETKPTKQSRGLGSDVSRGGHVSPMRLVLPVSISIIATAIIQQMVRNDGAS 420

Query: 421 GLKEHIGGSMALELVNSHWMQVIQTGVTWYVIGMAMMGMVEMIARKIR 469
            LKEH  GSMALELVNSHWMQVI TGVTWY+IGMA+MGM+EMIARK R
Sbjct: 421 ELKEHAAGSMALELVNSHWMQVILTGVTWYIIGMAVMGMLEMIARKFR 464

BLAST of Tan0022569 vs. ExPASy TrEMBL
Match: A0A5A7SY19 (Putative DNAJ heat shock N-terminal domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00570 PE=4 SV=1)

HSP 1 Score: 766.5 bits (1978), Expect = 6.3e-218
Identity = 387/468 (82.69%), Postives = 417/468 (89.10%), Query Frame = 0

Query: 1   MTAAWLPLYTPTAATKIQYPTQRKLGLYNSSTSKKLYGNTLSCRAASSITDFDLYDLLGI 60
           M+A+WLPLYTPT     QYP QRKLG YN STS+KL+ NTLSC+AASSITDFDLYDLLGI
Sbjct: 1   MSASWLPLYTPTK----QYPIQRKLGSYNPSTSRKLHANTLSCKAASSITDFDLYDLLGI 60

Query: 61  DNTSDPSRIKAAYRALQKQCHPDIAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMA 120
           DNTS+PSRIKAAYRALQK CHPDIAGPAGHDMAIILNE YSVLSDP+SRLAYDKEQAKMA
Sbjct: 61  DNTSNPSRIKAAYRALQKHCHPDIAGPAGHDMAIILNEAYSVLSDPSSRLAYDKEQAKMA 120

Query: 121 ELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180
           ELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW
Sbjct: 121 ELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQW 180

Query: 181 ADPEYKVTEAIEACPVDCISMVKRSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTD 240
           ADPEYKV EAIEACPVDCISMV+R+DLAALEFLMSKQPRGNVRVGMGN AGERVSNIFTD
Sbjct: 181 ADPEYKVMEAIEACPVDCISMVERTDLAALEFLMSKQPRGNVRVGMGNTAGERVSNIFTD 240

Query: 241 VKKFQTRFKEATEKTSKQHVKGTTFESEAQLSAIQAIRSISNWLFWQTATPTGTGSKHSQ 300
           VKKFQ RF EA EK +K+  KG TF+SE QL+AIQAIRSISNWLFWQTATP G GSK SQ
Sbjct: 241 VKKFQIRFNEAMEKATKEQSKGATFDSEGQLAAIQAIRSISNWLFWQTATPVGPGSKQSQ 300

Query: 301 NLTHIASKSTPEINKLQAAVTARKQLKAKTEDRNGATTKYIKKDDYWVPTTLALPVSTQA 360
           +LT  ASK TPEINKLQAAVTARKQ++ K E RN  TTKY+ +DDYWVPTT ALP STQ 
Sbjct: 301 SLTRSASKFTPEINKLQAAVTARKQIREKAEGRNRITTKYLSRDDYWVPTTFALPASTQC 360

Query: 361 PNSTISKPRVETKRTKESKDLGSEAGDGGHISPMRFALPVSISVIATAVIQQMVRDNGAN 420
           PN+ ISKP VETK TK+S+DLGS+   G H+SPMR  LPVSIS+IATA+IQQMVR++GA+
Sbjct: 361 PNNPISKPSVETKPTKQSRDLGSDVSSGVHVSPMRLILPVSISIIATAIIQQMVRNDGAD 420

Query: 421 GLKEHIGGSMALELVNSHWMQVIQTGVTWYVIGMAMMGMVEMIARKIR 469
            LKEH  GSMALELVNSHWMQVI TGVTWY+IGMA+ GMVEMIARK R
Sbjct: 421 ELKEHAAGSMALELVNSHWMQVILTGVTWYIIGMAVTGMVEMIARKFR 464

BLAST of Tan0022569 vs. TAIR 10
Match: AT5G23240.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 455.3 bits (1170), Expect = 6.0e-128
Identity = 245/449 (54.57%), Postives = 309/449 (68.82%), Query Frame = 0

Query: 31  STSKKLY----GNTLSCRA---ASSITDFDLYDLLGIDNTSDPSRIKAAYRALQKQCHPD 90
           STS+KL          CRA   +SSITDFDLYDLLGID +SD S+IK+AYRALQK+CHPD
Sbjct: 21  STSQKLITVRSPLKFKCRATSSSSSITDFDLYDLLGIDRSSDKSQIKSAYRALQKRCHPD 80

Query: 91  IAGPAGHDMAIILNEVYSVLSDPNSRLAYDKEQAKMAELRGYTGKPVYSVWLGSESEQRA 150
           IAG  GHDMAIILNE Y +LSDP SR AYDKEQAK+ ELRGYTGKP+YSVW G E+EQRA
Sbjct: 81  IAGDPGHDMAIILNEAYQLLSDPISRQAYDKEQAKLEELRGYTGKPIYSVWCGPETEQRA 140

Query: 151 VFVDEVKCIGCLKCALFAGKTFAVESVYGRARVVAQWADPEYKVTEAIEACPVDCISMVK 210
            FVDEVKC+GCLKCAL A KTFA+E+ YGRARVVAQWADPE K+ EAIEACPVDCISMV+
Sbjct: 141 AFVDEVKCVGCLKCALCAEKTFAIETAYGRARVVAQWADPESKIKEAIEACPVDCISMVE 200

Query: 211 RSDLAALEFLMSKQPRGNVRVGMGNAAGERVSNIFTDVKKFQTRFKEATEKTSKQHVKGT 270
           RSDLA LEFLMSKQPRGNVR+G+GN  GERVSN+F DVKKFQ R+ +A  +T+K+     
Sbjct: 201 RSDLAPLEFLMSKQPRGNVRIGVGNTVGERVSNVFVDVKKFQERYAKAMSRTTKE----- 260

Query: 271 TFESEAQLSAIQAIRSISNWLFWQTA---TPTGTGSKHSQNLTHIASKSTPEINKLQAAV 330
           T + E Q+SA++AIRSISNWL+W+++    P    S  S   T       P+I KLQ  V
Sbjct: 261 TSQREVQISAVEAIRSISNWLYWRSSPYTKPLSPESNMSLTFTKRKKAVDPDIRKLQDVV 320

Query: 331 TARKQL--KAKTEDRNGATTKYIKKDDYWVPTTLALPVSTQAPNSTISKPRVETKRTKES 390
            A KQ     +T+++  A   Y+  +DYW P+  ALP S     S  S     T++T  S
Sbjct: 321 AAMKQADQSGRTKEKGSA---YLLGEDYWSPSNAALPSSGNNNGSKASSNPQVTRKTFPS 380

Query: 391 KDLGSEAGDGGHISPMRFALPVSISVIATAVIQQMVRDNGANGLKEHIGGSMALELVNSH 450
           ++  +   +      ++   P+  +++A  ++Q       A+ L +HIGGS+AL +VNS 
Sbjct: 381 EEKPTSRRENRRQFRIK-KFPIGTAIVAVFLVQYQASYRAASELNDHIGGSLALSIVNSP 440

Query: 451 WMQVIQTGVTWYVIGMAMMGMVEMIARKI 468
           W Q++  GVTWY IG  ++ +VE +  K+
Sbjct: 441 WQQILLAGVTWYFIGAMLLQLVEAVQHKL 460

BLAST of Tan0022569 vs. TAIR 10
Match: AT2G42750.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 119.0 bits (297), Expect = 1.0e-26
Identity = 69/183 (37.70%), Postives = 98/183 (53.55%), Query Frame = 0

Query: 53  DLYDLLGIDNTSDPSRIKAAYRALQKQCHPDIAG--PAGHDMAIILNEVYSVLSDPNSRL 112
           D Y +LG+   +    IK AY    K CHPD++G  P   +  + +N++Y +LSDP  R+
Sbjct: 76  DYYAVLGLLPDATQEEIKKAYYNCMKSCHPDLSGNDPETTNFCMFINDIYEILSDPVQRM 135

Query: 113 AYDKEQAKMAELRGYTGKPVYSVWLGSESEQRAVFVDEVKCIGCLKCALFAGKTFAVESV 172
            YD       E+ GYT   + + +L   + +  VFVDE  CIGC  CA  A   F +E  
Sbjct: 136 VYD-------EIHGYTVTAI-NPFLDDSTPRDHVFVDEFACIGCKNCANVAPDIFQIEED 195

Query: 173 YGRARVVAQWADPEYKVTEAIEACPVDCISMVKRSDLAALEFLMSKQPRGNVRV---GMG 231
           +GRAR   Q  +P+  V +A+E CPVDCI     + L+ LE  M +  R NV +   GMG
Sbjct: 196 FGRARACNQRGNPDL-VQQAVETCPVDCIHQTSAAQLSLLEDEMRRVERVNVALMLSGMG 249

BLAST of Tan0022569 vs. TAIR 10
Match: AT3G05345.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 65.9 bits (159), Expect = 1.0e-10
Identity = 52/189 (27.51%), Postives = 84/189 (44.44%), Query Frame = 0

Query: 31  STSKKLYGNTLSCRAASSITDFDLYDLLGIDNTSDPSRIKAAYRALQKQCHPDIAGPAGH 90
           STS++   +         ++    Y +LG++ +   S +KAA+RA  KQ HPD+     +
Sbjct: 22  STSQRFIPSCRGKNREDPLSSSSPYSILGVEPSCSSSELKAAFRAKVKQYHPDVNKDGSN 81

Query: 91  DMAII--LNEVYSVLSDPNSRLAYDKEQAKMAELRGYTGKPVYSVWLGSESEQRAVFVDE 150
              +I  + + Y +L++      Y + +    E       P        E E   VFV+E
Sbjct: 82  SDIMIRRIIQAYEMLTN------YSRSEIIEGECLDPFDHP--------ECEALDVFVNE 141

Query: 151 VKCIG---CLKCALFAGKTFAVESVYGRARVVAQWADPEYKVTEAIEACPVDCISMVKRS 210
           V C+G      C   A   F+ +S  G AR ++Q    +Y+V  A+  CP +CI  V  S
Sbjct: 142 VLCVGKRCSYPCFETASHVFSCDS-SGTARAMSQGHGEDYRVQSAVNQCPRNCIHYVTPS 195

Query: 211 DLAALEFLM 215
               LE L+
Sbjct: 202 QRIILEELL 195

BLAST of Tan0022569 vs. TAIR 10
Match: AT1G80030.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 53.5 bits (127), Expect = 5.2e-07
Identity = 32/91 (35.16%), Postives = 44/91 (48.35%), Query Frame = 0

Query: 25  LGLYNSSTSKKLYGNTLSCRAASSITDFDLYDLLGIDNTSDPSRIKAAYRALQKQCHPDI 84
           L L +SS+S   Y N          T  D Y  LG+  +++   IKAAYR L +Q HPD+
Sbjct: 47  LSLSSSSSSSTPYRNRRGRSLVVFATSGDYYATLGVSKSANNKEIKAAYRRLARQYHPDV 106

Query: 85  -AGPAGHDMAIILNEVYSVLSDPNSRLAYDK 115
              P   +    ++  Y VLSD   R  YD+
Sbjct: 107 NKEPGATEKFKEISAAYEVLSDEQKRALYDQ 137

BLAST of Tan0022569 vs. TAIR 10
Match: AT1G80030.3 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 53.5 bits (127), Expect = 5.2e-07
Identity = 32/91 (35.16%), Postives = 44/91 (48.35%), Query Frame = 0

Query: 25  LGLYNSSTSKKLYGNTLSCRAASSITDFDLYDLLGIDNTSDPSRIKAAYRALQKQCHPDI 84
           L L +SS+S   Y N          T  D Y  LG+  +++   IKAAYR L +Q HPD+
Sbjct: 47  LSLSSSSSSSTPYRNRRGRSLVVFATSGDYYATLGVSKSANNKEIKAAYRRLARQYHPDV 106

Query: 85  -AGPAGHDMAIILNEVYSVLSDPNSRLAYDK 115
              P   +    ++  Y VLSD   R  YD+
Sbjct: 107 NKEPGATEKFKEISAAYEVLSDEQKRALYDQ 137

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMX68.4e-12754.57Chaperone protein dnaJ C76, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DJC... [more]
Q8G6C67.9e-0844.26Chaperone protein DnaJ OS=Bifidobacterium longum (strain NCC 2705) OX=206672 GN=... [more]
B5YAR41.0e-0742.42Chaperone protein DnaJ OS=Dictyoglomus thermophilum (strain ATCC 35947 / DSM 396... [more]
Q7V9C81.3e-0743.59Chaperone protein DnaJ OS=Prochlorococcus marinus (strain MIT 9313) OX=74547 GN=... [more]
Q0AKB32.3e-0745.31Chaperone protein DnaJ OS=Maricaulis maris (strain MCS10) OX=394221 GN=dnaJ PE=3... [more]
Match NameE-valueIdentityDescription
KAG6600118.11.1e-22988.68Chaperone protein dnaJ C76, chloroplastic, partial [Cucurbita argyrosperma subsp... [more]
XP_022942611.12.1e-22888.25chaperone protein dnaJ C76, chloroplastic-like [Cucurbita moschata][more]
XP_023532693.13.1e-22787.82chaperone protein dnaJ C76, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022987962.11.6e-22386.75chaperone protein dnaJ C76, chloroplastic-like [Cucurbita maxima][more]
XP_023543201.16.7e-22284.43chaperone protein dnaJ C76, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1FPC31.0e-22888.25chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1JBT27.7e-22486.75chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1GZ806.1e-22184.01chaperone protein dnaJ C76, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=... [more]
A0A0A0KPY45.7e-21982.91J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G175710 PE=4 SV=1[more]
A0A5A7SY196.3e-21882.69Putative DNAJ heat shock N-terminal domain-containing protein OS=Cucumis melo va... [more]
Match NameE-valueIdentityDescription
AT5G23240.16.0e-12854.57DNAJ heat shock N-terminal domain-containing protein [more]
AT2G42750.11.0e-2637.70DNAJ heat shock N-terminal domain-containing protein [more]
AT3G05345.11.0e-1027.51Chaperone DnaJ-domain superfamily protein [more]
AT1G80030.15.2e-0735.16Molecular chaperone Hsp40/DnaJ family protein [more]
AT1G80030.35.2e-0735.16Molecular chaperone Hsp40/DnaJ family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 52..108
e-value: 3.6E-14
score: 63.1
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 53..113
e-value: 2.2E-13
score: 50.1
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 53..116
score: 14.515092
IPR001623DnaJ domainCDDcd06257DnaJcoord: 53..105
e-value: 3.35845E-14
score: 64.8741
NoneNo IPR availablePFAMPF13370Fer4_13coord: 146..200
e-value: 3.5E-11
score: 43.5
NoneNo IPR availableGENE3D3.30.70.20coord: 141..203
e-value: 3.9E-15
score: 57.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 359..388
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 369..384
NoneNo IPR availablePANTHERPTHR45295CHAPERONE PROTEIN DNAJ C76, CHLOROPLASTICcoord: 34..466
NoneNo IPR availablePANTHERPTHR45295:SF1CHAPERONE PROTEIN DNAJ C76, CHLOROPLASTICcoord: 34..466
NoneNo IPR availableSUPERFAMILY548624Fe-4S ferredoxinscoord: 143..202
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 40..127
e-value: 1.2E-15
score: 59.4
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 50..119

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0022569.1Tan0022569.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane