Tan0022520 (gene) Snake gourd v1

BLAST of Tan0022520 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 972.6 bits (2513), Expect = 3.1e-282
Identity = 552/921 (59.93%), Postives = 683/921 (74.16%), Query Frame = 0

Query: 49  DASDRREVIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFWNDSDGRRNNGEG---- 108
           DA+DRREVIVKID  + ++N  S   E+V   G+IWR+ SYDFW D +G  N G      
Sbjct: 4   DAADRREVIVKIDGENGNNNGVSG--ETV---GKIWRDGSYDFWTDGEGNLNKGHNAAAV 63

Query: 109 --------SNGDGRSEDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELP 168
                   +    + E FEFR+     EDPP+KLIGQFLHKQ+ASGE+ LDMD+ M EL 
Sbjct: 64  DSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGMDELQ 123

Query: 169 LDKTLPPVAESPMRRSSRELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDR 228
             + L PV+ESP        +VS       ++ D + RR                   D 
Sbjct: 124 -SRGLTPVSESP--------RVS-------TKRDPVGRR-------------------DS 183

Query: 229 RAYGNGHDDSGDEVVRCTSNSS-FQRSVSFQRKSSLLRAKTKSRLLD------PPEQPDR 288
           R+  N +DD   EVV+C+ N++  QRS      S+LL+ +T+SRL D      PP+  D 
Sbjct: 184 RSNTNNNDDG--EVVKCSGNNAPIQRS-----SSTLLKMRTRSRLSDPPTPQLPPQTADM 243

Query: 289 RSGRVPKSGQMRSGLLSKAL----DEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILI 348
           +SGR+PKSGQM+SG   K+     +EE+DDPF  EDLP+EY+K  L +  +L+W SLILI
Sbjct: 244 KSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILI 303

Query: 349 IAALVCTLTIPYWRQKNLWKLKLWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKR 408
           IA  VCTL IP  R+K LW+L+LWKWE M+LVLICGRLVS W ++I+VFFIERNFLLRKR
Sbjct: 304 IAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKR 363

Query: 409 VLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVTTLVWL 468
           VLYFVYGVRKAVQNCLWLGLVL+AW+FLFD+KV +   + AL  VTK+ VCLLV  L+WL
Sbjct: 364 VLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVGFLLWL 423

Query: 469 VKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKL 528
           VKTL+VKVLASSFH+STYFDRIQ++LF QYVIETLSGPPLIEIQKNEEEEER++ EV K 
Sbjct: 424 VKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKF 483

Query: 529 QNAGATIPPDLKATAFSTPKGGRLIGSGGLQRSP--RGRSAKLSRVLSKKG-----DEGI 588
           QN                  GG  I SG  Q+SP   G+S  LS VLS  G     ++GI
Sbjct: 484 QN-----------------PGGVEIQSGA-QKSPMKTGKSPFLSHVLSNGGGGGGENKGI 543

Query: 589 TIDHLHKLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSTHEEDESATQIRSEFEAKVA 648
           TID LHKL+PKNVSAW MKRLMNI+R+G+L+TLDEQ++D + ++D+   QIRSEFEAK+A
Sbjct: 544 TIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDK-GNQIRSEFEAKLA 603

Query: 649 AKKIFQNVARHGSKYIYLEDLMRFMQEDEASKTMSLFEGASESRKISKSSLKNWVVNAFR 708
           A+KIF NVA+ GSK+IY  D+MRF+ +DEA KT+SLFEGASE+ +ISKSSLKNWVVNAFR
Sbjct: 604 ARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAFR 663

Query: 709 ERRALALTLNDTKTAVNKLHRMVNILVSVVILVIWLLILGIATSKFLLFVTSQLVLVAFV 768
           ERRALALTLNDTKTAVN+LH+MVNI+V ++ILVIWL+ILGI ++KFL+ ++SQ+V+VAF+
Sbjct: 664 ERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFI 723

Query: 769 FGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSV 828
           FGN CK VFE+II+LFV+HPFDVGDRCEIDGVQM+VEEMNILTT+FLR+DNQK+++PNS+
Sbjct: 724 FGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSL 783

Query: 829 LATKAIHNYYRSPDMGDSVEFCLHISTPPEKIAVMRQRIVSYIEGKKEHWCSTPMIVLKD 888
           L TK+I NYYRSPDMGD +EF +HI+TP EKI +++QRI SYIEGKK+HW   PMIV KD
Sbjct: 784 LWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKD 843

Query: 889 VEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLP 935
           +E LN +RIA+W THRMNHQDMGE+W RR+ LVEE+ KI +ELD++YRL PLDINVR+LP
Sbjct: 844 MESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINVRNLP 853

BLAST of Tan0022520 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 930.2 bits (2403), Expect = 1.8e-269
Identity = 520/917 (56.71%), Postives = 672/917 (73.28%), Query Frame = 0

Query: 45  MAPSDASDRREVIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFWNDSDGRRN-NGE 104
           MA  D++DRR+ IV I+  + S    +    S    G IW+ESSYDFW+   G+ +  G+
Sbjct: 1   MAAVDSTDRRDFIVNINGQE-SGAVGATGSSSNAEGGNIWKESSYDFWDGEKGKNDKKGD 60

Query: 105 GSNGDGRSEDFEFRQHRK----DVEDPPSKLIGQFLHKQKASG-EMSLDMDMEMLELPLD 164
             + DG S  F  R  R+    ++ DPPSKLIGQFLHKQ+ASG E+SLD+++ M EL  +
Sbjct: 61  DEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN 120

Query: 165 -KTLPPVAESPMRRSSRELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRR 224
               P  A +  RR    +  S   +    + D++RRR      +    GG   ++G  R
Sbjct: 121 TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQ-----NRTSLGGSSDEEGRNR 180

Query: 225 AYGNGHDDSGDEVVRCTSNSSFQRSVSFQRKSSLLRAKTKSRLLDPP-------EQPDRR 284
                 D++  EV++C S           +K  L R KTKSRL DPP       ++ + +
Sbjct: 181 ------DEA--EVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHPAIDKTEMK 240

Query: 285 SGRVPKSGQMRSGLLSKA-----------LDEEDDDPFLEEDLPDEYKKANLGVLTLLQW 344
           SGR  +SG  +SG L K+            +EE++DPFL+EDLP+E+K+  L     L+W
Sbjct: 241 SGR--RSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFLEW 300

Query: 345 ASLILIIAALVCTLTIPYWRQKNLWKLKLWKWEVMILVLICGRLVSGWGIRIIVFFIERN 404
            SL+LI+ +LVC+LTI   ++K  WKL LWKWEV +LVLICGRLVS W +RIIVF +E+N
Sbjct: 301 ISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKN 360

Query: 405 FLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLV 464
           F  RKRVLYFVYGVRK+VQNCLWLGLVL+AW+FLFD KV+RE +S AL YVT+VLVCLLV
Sbjct: 361 FTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVLVCLLV 420

Query: 465 TTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLA 524
             ++WLVKT++VKVLASSFH+STYFDRIQ++LF QYVIETLSGPPL+EIQ+ EEEE+++A
Sbjct: 421 ALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVA 480

Query: 525 EEVIKLQN-AGATIPPDLKATAFSTPKGGRLIGSGGLQRSPRGRSAKLSRVLSKKGD--E 584
           E+V  L+  AGA +PP LKAT  S  K               G+S  L+R+ SK+G+  E
Sbjct: 481 EDVKSLEKLAGAKLPPALKATVKSFMK--------------VGKSPGLNRIGSKRGEDGE 540

Query: 585 GITIDHLHKLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSTHEEDESATQIRSEFEAK 644
           GI ID L +++ KNVSAWNMKRLMNI+  GA+STLD+ ++D+T +EDE AT IRSE+EAK
Sbjct: 541 GIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTT-QEDEDATHIRSEYEAK 600

Query: 645 VAAKKIFQNVARHGSKYIYLEDLMRFMQEDEASKTMSLFEGASESRKISKSSLKNWVVNA 704
            AA+KIF NV   GS+YIYLED +RF+ E+EA + M+LFEGASES KISKS LKNWVV A
Sbjct: 601 CAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKA 660

Query: 705 FRERRALALTLNDTKTAVNKLHRMVNILVSVVILVIWLLILGIATSKFLLFVTSQLVLVA 764
           FRERRALALTLNDTKTAV++LHR++N+++ ++I++IWLLILGIAT++FLL ++SQL+LVA
Sbjct: 661 FRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVA 720

Query: 765 FVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPN 824
           FVFGN+CKT+FEAIIFLFVMHPFDVGDRCEIDGVQ++VEEMNILTT+FLRYDNQKII+PN
Sbjct: 721 FVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPN 780

Query: 825 SVLATKAIHNYYRSPDMGDSVEFCLHISTPPEKIAVMRQRIVSYIEGKKEHWCSTPMIVL 884
           SVL TK I NYYRSPDMGD+VEFC+HI+TPPEKI  ++QRI+SY++ KK++W   PMIV 
Sbjct: 781 SVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVF 840

Query: 885 KDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRS 930
             +++LN ++IA+WLTHRMNHQDMGER+ RR LL+EE+ K  +ELD++YRL PL+INVRS
Sbjct: 841 LSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVRS 875

BLAST of Tan0022520 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 927.5 bits (2396), Expect = 1.1e-268
Identity = 522/915 (57.05%), Postives = 662/915 (72.35%), Query Frame = 0

Query: 45  MAPSDASDRREVIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFW-NDSDGRRNNGE 104
           MA      RR+ +V+ID  D   N  S          + WRESS +FW ND   +   GE
Sbjct: 1   MAVDSTDQRRDFVVRIDGED---NGDSE---------KFWRESSINFWHNDKSSKPPGGE 60

Query: 105 GSNGDGRSEDFEFRQHRKDVE-DPPSKLIGQFLHKQKASG-EMSLDMDMEMLELPLDKTL 164
             +G   S DF  R   K  E DPPSKLI QFL+KQKASG E+SLDM+  M EL    T+
Sbjct: 61  EDDG---SFDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPELQ-KNTV 120

Query: 165 PPVAESPMRRSSRELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRRAYGN 224
           PP++ + +  S+  +     +       D+IRRR          + G  S+  + R    
Sbjct: 121 PPLSSTAVSGSASPVTAPVTASYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRV--- 180

Query: 225 GHDDSGDEVVRCTSNSSFQRSVSFQRKSSLLRAKTKSRLLDP--PEQPDRRSGRVPKSGQ 284
                G EVV+CTSN S  R+       +L++ KT+SRL+DP  P  PD  SGR P+SG 
Sbjct: 181 ----DGSEVVKCTSNRSTMRT------KTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGN 240

Query: 285 MRSGLL-------------SKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILII 344
           +  G               SK L EE++DPF EEDLP+  +K  + V  +++W  LILII
Sbjct: 241 LNPGFSGRNTKPGTPNQGGSKDL-EEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILII 300

Query: 345 AALVCTLTIPYWRQKNLWKLKLWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRV 404
           A+L+C+L IPY R K LW L LWKWEVM+LVLICGRLVS W +++ V+F+E NFL RK+V
Sbjct: 301 ASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKV 360

Query: 405 LYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVTTLVWLV 464
           LYFVYG+RK VQNCLWLGLVLIAW+FLFD KV+RE++S  L+YVTKVL+CLLV  ++WL+
Sbjct: 361 LYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLI 420

Query: 465 KTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQ 524
           KTL+VKVLASSFH+STYFDRIQ++LF QYVIETLSGPP IEI     EEE++A +V   +
Sbjct: 421 KTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEI---HIEEEKVANDVKTFE 480

Query: 525 NAGATIPPDLKATAFSTPKGGRLIGSGGLQRSPR--GRSAKLSRVLSKK--GDEGITIDH 584
             G  + P L   A S+P     +GSG LQ+SP   G+S  LSR  SKK  G+EGI IDH
Sbjct: 481 IVGRKLSP-LGPKAVSSPP-QVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDH 540

Query: 585 LHKLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSTHEEDESATQIRSEFEAKVAAKKI 644
           L +++ KNVSAW MK+LMN+++ G LSTLDEQI+D+T +ED+ ATQIRSEFEAK+AA+KI
Sbjct: 541 LQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKI 600

Query: 645 FQNVARHGSKYIYLEDLMRFMQEDEASKTMSLFEGASESRKISKSSLKNWVVNAFRERRA 704
           FQNVA  GS+YIY+ED MRF+ EDE+ + M LFEGASE  KISKS LKNWVVNAFRERRA
Sbjct: 601 FQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRA 660

Query: 705 LALTLNDTKTAVNKLHRMVNILVSVVILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNT 764
           LALTLNDTKTAVN+LHR+V++LVS+VIL+IWLLILGIAT+KFLL ++SQL+LV FVFGN+
Sbjct: 661 LALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNS 720

Query: 765 CKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATK 824
           CKT+FEA+IF+FVMHPFDVGDRCEIDGVQMIVEEMNILTT+FLR+DNQKI++PNS+L TK
Sbjct: 721 CKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTK 780

Query: 825 AIHNYYRSPDMGDSVEFCLHISTPPEKIAVMRQRIVSYIEGKKEHWCSTPMIVLKDVEEL 884
            I NYYRSPDM D++EF +HI+TPPEK   +RQRI+SY++ KK+HW  +PMIV +D+  L
Sbjct: 781 PIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGL 840

Query: 885 NRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLP---P 935
           N ++IA+W TH+MNHQ+MGER+ RR  L+EE+ ++ +ELD++YRL PL+INV+SLP   P
Sbjct: 841 NSVKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATP 880

BLAST of Tan0022520 vs. ExPASy Swiss-Prot
Match: F4IME2 (Mechanosensitive ion channel protein 8 OS=Arabidopsis thaliana OX=3702 GN=MSL8 PE=2 SV=2)

HSP 1 Score: 923.3 bits (2385), Expect = 2.2e-267
Identity = 526/958 (54.91%), Postives = 681/958 (71.09%), Query Frame = 0

Query: 1   MDFSLKSFKSHGSYKYVRKLSGDQ-----EQLPILSDQEPDNLHRNHSIMAPSDASDRRE 60
           MDF   SFKSH SYK +R   GDQ     E LPIL D  PD     HS M   D      
Sbjct: 1   MDFR-NSFKSHSSYKQIRS-PGDQSEPSPEHLPILHDHHPD-----HSGMVVDD------ 60

Query: 61  VIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFWNDSDGRRNNGE------------ 120
                         S+R+      N  + R++SY FW D+    +               
Sbjct: 61  ----------QKPDSTRSSLDDGRNAPVERDASYKFWQDNTTGTSTDHTAVRTSDKDPIA 120

Query: 121 -GSNGDGRSEDFEFRQHRKDVEDPPSKLI-GQFLHKQ---KASGEMSLDMDMEMLELPLD 180
               GD  S  F+F   +  V++ P+K++ G+ +++Q   + + E++LD+D E       
Sbjct: 121 ISRKGDRLSGSFDFVHGKLPVDESPTKMVAGEPVNRQWRGRNNEEITLDVDQEN------ 180

Query: 181 KTLPPVAESPMRRSSRELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRRA 240
                V+   M   +   + SF++  E+  + ++RR      A         S       
Sbjct: 181 ---DDVSHQTMPTPTSTARTSFDASREMRVSFNVRRAGGAFVAGSVPSSSSHSSSSSSAT 240

Query: 241 YGNGHDD---SGDEVVRCTSNSSFQRSVSFQRKSSLL-RAKTKSRLLDPP-EQPDRRSGR 300
                D      +EVVRCTSN      +SFQRKS L+ R KT+SRL DPP E+    SG 
Sbjct: 241 MRTNQDQPQLQEEEVVRCTSN------MSFQRKSELISRVKTRSRLQDPPREEETPYSGW 300

Query: 301 VPKSGQMRSGLLSKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTL 360
             +SGQ++SGLL+    +E+DDP  EED+PDEYK+  L  +TLLQW SL+ IIAAL C+L
Sbjct: 301 --RSGQLKSGLLADI--DEEDDPLAEEDVPDEYKRGKLDAITLLQWLSLVAIIAALACSL 360

Query: 361 TIPYWRQKNLWKLKLWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGV 420
           +I  W++  +W L LWKWEV +LVLICGRLVSGWGIRI+VFFIERNFLLRKRVLYFVYGV
Sbjct: 361 SIQSWKKVRVWNLHLWKWEVFLLVLICGRLVSGWGIRIVVFFIERNFLLRKRVLYFVYGV 420

Query: 421 RKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVTTLVWLVKTLMVKV 480
           R+AVQNCLWLGLVL+AW+FLFD KVQRE +S  L YVTK+LVC L++T++WL+KTL+VKV
Sbjct: 421 RRAVQNCLWLGLVLLAWHFLFDKKVQRETRSRFLPYVTKILVCFLLSTILWLIKTLVVKV 480

Query: 481 LASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIP 540
           LASSFHVSTYFDRIQ+ALFNQYVIETLSGPP+IE+ + EEEEER  +E+ K+QNAGA +P
Sbjct: 481 LASSFHVSTYFDRIQEALFNQYVIETLSGPPMIEMSRIEEEEERAQDEIFKMQNAGANLP 540

Query: 541 PDLKATAFSTPKGGRLIGSGGLQRSPRGRSAKLSRVLSKK-GDEGITIDHLHKLSPKNVS 600
           PDL A AF   K GR++            + KLS ++ K   D GI+++HLH+++ KN+S
Sbjct: 541 PDLCAAAFPPGKSGRVM------------NPKLSPIIPKSTTDNGISMEHLHRMNHKNIS 600

Query: 601 AWNMKRLMNIVRHGALSTLDEQIKDSTHEEDESATQIRSEFEAKVAAKKIFQNVARHGSK 660
           AWNMKRLM IVR+ +L+TLDEQ+ +ST+ EDES  QIRSE EAK AA+KIF+NV + G+K
Sbjct: 601 AWNMKRLMKIVRNVSLTTLDEQMLESTY-EDESTRQIRSEKEAKAAARKIFKNVEQRGAK 660

Query: 661 YIYLEDLMRFMQEDEASKTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKT 720
           YIYLEDLMRF++EDEA KTM LFEGA E+++ISKS+LKNW+VNAFRERRALALTLNDTKT
Sbjct: 661 YIYLEDLMRFLREDEAMKTMGLFEGAPENKRISKSALKNWLVNAFRERRALALTLNDTKT 720

Query: 721 AVNKLHRMVNILVSVVILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIF 780
           AVNKLH M+NI+ ++VI+VIWL++L IA+SK LLFV+SQ+VL+AF+FGNT KTVFE+IIF
Sbjct: 721 AVNKLHHMINIVTAIVIVVIWLVLLEIASSKVLLFVSSQVVLLAFIFGNTVKTVFESIIF 780

Query: 781 LFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNYYRSPD 840
           LF++HP+DVGDRCEID VQ++VEEMNILTT+FLRYDN KI++PNS+L  K+I+NYYRSPD
Sbjct: 781 LFIVHPYDVGDRCEIDSVQLVVEEMNILTTVFLRYDNLKIMYPNSLLWQKSINNYYRSPD 840

Query: 841 MGDSVEFCLHISTPPEKIAVMRQRIVSYIEGKKEHWCSTPMIVLKDVEELNRMRIAIWLT 900
           MGD++EFC+HI+TP EKI+V++QRI +YI+ K E+W     I++KD+E+L+ +R+AIW  
Sbjct: 841 MGDAIEFCVHITTPLEKISVIKQRISNYIDNKPEYWYPQAKIIVKDLEDLHIVRLAIWPC 900

Query: 901 HRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLPPVNSTRLPATWT 931
           HR+NHQDM ERWTRRA+LVEE++KI  ELD+Q+R  PLDINVR++P V S+R+P  W+
Sbjct: 901 HRINHQDMAERWTRRAVLVEEVIKILLELDIQHRFYPLDINVRTMPTVVSSRVPPGWS 903

BLAST of Tan0022520 vs. ExPASy Swiss-Prot
Match: F4IME1 (Mechanosensitive ion channel protein 7 OS=Arabidopsis thaliana OX=3702 GN=MSL7 PE=2 SV=1)

HSP 1 Score: 817.4 bits (2110), Expect = 1.7e-235
Identity = 472/891 (52.97%), Postives = 625/891 (70.15%), Query Frame = 0

Query: 51  SDRREVIVKIDQSDAS------SNASSRAVESVNSNGRIWRESSYDFWNDSDGRRNNGEG 110
           S  +++I   DQ++ +      +N     +    S+G  +  +SY FW D          
Sbjct: 11  SSYKQIISTGDQNEKTKKKKKLANLDDGDIAKTQSSGSSFDGNSYKFWQDI--------A 70

Query: 111 SNGDGRSEDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPLDKTLPPV 170
           ++   +S  F+F Q+R+                     E++LD++ E  E         V
Sbjct: 71  TDDYTKSGSFDFPQYRE---------------------EITLDVNEETEE------TEDV 130

Query: 171 AESPMRRSSRELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRRAYGNGHD 230
           + +     S+E +V F+  S  + N S   R   S           S    R       +
Sbjct: 131 SNNNNLSGSKETRVFFKINSSGTNNMSGSVRSCTSST-------SFSSATMRLNLEQQLE 190

Query: 231 DSGDEVVRCTSNSSFQRSVSFQRKSSLL-RAKTKSRLLDPPEQPDRR-SGRVPKSGQMRS 290
           D G+ VVRC+S           RK+ L+ RAK +SRL+DPP++ +++ S  +  S Q+RS
Sbjct: 191 DEGEVVVRCSS----------VRKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRS 250

Query: 291 GLLSKALD--EEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIPYWRQ 350
           GLL +  D  EE+DD   EED+P EY+K  +  +TLLQW SLI ++ ALV +L +  WR 
Sbjct: 251 GLLGRHSDDIEEEDDSSAEEDVPVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRN 310

Query: 351 KNLWKLKLWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNC 410
             LW L LWKWEV++LVLICGRLVSG GIRIIVFFIERNFLLRKRVLYFVYGV+ AVQNC
Sbjct: 311 ATLWSLHLWKWEVVLLVLICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNC 370

Query: 411 LWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVTTLVWLVKTLMVKVLASSFHV 470
           LWLGLVL+AW+FLFD KV++E +S+ L  ++K+LVC L++T++WL+KTL+VKVLASSFHV
Sbjct: 371 LWLGLVLLAWHFLFDKKVEKETQSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHV 430

Query: 471 STYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATA 530
           STYFDRIQ+ALF+ Y+IETLSGPP++E+ + EEEE+R  +E+ K+Q  GA + P+L + A
Sbjct: 431 STYFDRIQEALFHHYLIETLSGPPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAA 490

Query: 531 FSTPKGGRLIGSGGLQRSPRGRSAKLSRVLSKKG-DEGITIDHLHKLSPKNVSAWNMKRL 590
           F   K G  +            + K S ++ K G D GIT+D LHK++ KNVSAWNMKRL
Sbjct: 491 FPQEKSGSTM------------NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRL 550

Query: 591 MNIVRHGALSTLDEQIKDSTHEEDESATQIRSEFEAKVAAKKIFQNVARHGSKYIYLEDL 650
           M IVR+ +LSTLDEQ   +T  EDES  QIRSE EAK AA+KIF+NVA+ G+K+IYLEDL
Sbjct: 551 MKIVRNVSLSTLDEQALQNTC-EDESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDL 610

Query: 651 MRFMQEDEASKTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHR 710
           MRF++ DEA KTM LFEGA  ++KI+KS+LKNW+VNAFRERRALALTLNDTKTAVNKLH 
Sbjct: 611 MRFLRVDEAMKTMCLFEGALVTKKITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHH 670

Query: 711 MVNILVSVVILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPF 770
           M++ L ++VI+VIWL++L IATSK+LLF+TSQ+VL+AF+FGN+ KTVFE+IIFLF++HP+
Sbjct: 671 MISFLTAIVIIVIWLILLEIATSKYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPY 730

Query: 771 DVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNYYRSPDMGDSVEF 830
           DVGDR  ID V+M+VEEMNILTT+FLR DN KI++PN +L  KAIHNY RSPDMGD V  
Sbjct: 731 DVGDRLLIDTVEMVVEEMNILTTVFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTC 790

Query: 831 CLHISTPPEKIAVMRQRIVSYIEGKKEHWCSTPMIVLKDVEELNRMRIAIWLTHRMNHQD 890
           C+HI+TPPEKIA ++QRI SYI+ K E+W     +++KDVE+LN +RIAIWL H++NHQ+
Sbjct: 791 CVHITTPPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQN 836

Query: 891 MGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLPPVNSTRLPATWT 931
           MGER+TRRALL+EE++KI  ELD+QYR  PLDINV+++P V S+R+P  W+
Sbjct: 851 MGERFTRRALLIEEVIKILLELDIQYRFHPLDINVKTMPTVVSSRVPPAWS 836

BLAST of Tan0022520 vs. NCBI nr
Match: XP_022140409.1 (mechanosensitive ion channel protein 6-like [Momordica charantia])

HSP 1 Score: 1632.8 bits (4227), Expect = 0.0e+00
Identity = 865/945 (91.53%), Postives = 896/945 (94.81%), Query Frame = 0

Query: 1   MDFSLKSFKSHGSYKYVRKLSG------DQEQLPILSDQEPDNLHRNHSIMAPSDASDRR 60
           MDFSLKSFKSHGSYKY RKLSG       QEQLPILSDQ+P NLH+  S MA SDASDR 
Sbjct: 1   MDFSLKSFKSHGSYKYARKLSGGGGADDSQEQLPILSDQDPGNLHQQQS-MAVSDASDRN 60

Query: 61  EVIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFWNDSDGRRNNGEGSNGDGRSEDF 120
           EVIVKIDQ D S NA+SR +E+VN++GRIWRESSYDFWND DGRR+N EGSNGDGRSEDF
Sbjct: 61  EVIVKIDQPD-SLNATSRVMEAVNASGRIWRESSYDFWNDGDGRRSNAEGSNGDGRSEDF 120

Query: 121 EFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPLDKTLPPVAESPMRRSSR 180
           EFRQHR+ VEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELP DKTLPPVAESPMRRSS+
Sbjct: 121 EFRQHRQGVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTLPPVAESPMRRSSK 180

Query: 181 ELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRRAYGNGHDDSGDEVVRCT 240
           ELKVSFESISEISENDS+RRRHRDSPADEE++G Q  Q+ DRRAYGNGHDD G EVVRCT
Sbjct: 181 ELKVSFESISEISENDSMRRRHRDSPADEEYKGQQPPQR-DRRAYGNGHDDGGAEVVRCT 240

Query: 241 SNSSFQRSVSFQRKSSLLRAKTKSRLLDPPEQPDR----RSGRV-PKSGQMRSGLLSKAL 300
           SNSSFQR VSFQRKSSLLRAKTKSRLLDPPEQPDR    RSGRV PKSGQ+RSGLLSKAL
Sbjct: 241 SNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEQPDRRSGVRSGRVPPKSGQIRSGLLSKAL 300

Query: 301 DEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIPYWRQKNLWKLKLW 360
           DEEDDDPFLEEDLPDEYKK+NLG LTLLQWASLILIIAALVCTL+IPY  +KNLWKLKLW
Sbjct: 301 DEEDDDPFLEEDLPDEYKKSNLGALTLLQWASLILIIAALVCTLSIPYLNRKNLWKLKLW 360

Query: 361 KWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIA 420
           KWEVM+LVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIA
Sbjct: 361 KWEVMVLVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIA 420

Query: 421 WNFLFDDKVQREVKSNALEYVTKVLVCLLVTTLVWLVKTLMVKVLASSFHVSTYFDRIQD 480
           WNFLFDDKVQREVKSNALEYVTKVLVCLLV+TLVWLVKTLMVKVLASSFHVSTYFDRIQD
Sbjct: 421 WNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQD 480

Query: 481 ALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTPKGGRL 540
           ALFNQYVIETLSGPPLIEI+KNEEEEERLAEEV+KLQNAGATIPPDLKATAFST KGGR+
Sbjct: 481 ALFNQYVIETLSGPPLIEIRKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAKGGRV 540

Query: 541 IGSGGLQRSPRGRSAKLSRVLSKKGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGALS 600
           IGSGGLQRSPRGRS KLSR LSK GD+GITIDHLHKLSPKNVSAWNMKRLMNIVRHGALS
Sbjct: 541 IGSGGLQRSPRGRSGKLSRSLSKNGDDGITIDHLHKLSPKNVSAWNMKRLMNIVRHGALS 600

Query: 601 TLDEQIKDSTHEEDESATQIRSEFEAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEAS 660
           TLDEQIKDSTHEEDESATQI+SE EAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEA 
Sbjct: 601 TLDEQIKDSTHEEDESATQIKSECEAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEAL 660

Query: 661 KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVI 720
           KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVI
Sbjct: 661 KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVI 720

Query: 721 LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG 780
           LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG
Sbjct: 721 LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG 780

Query: 781 VQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNYYRSPDMGDSVEFCLHISTPPEK 840
           VQMIVEEMNILTTIFLRYDNQKI+FPNSVLATKAIHN+YRSPDMGD+VEFCLHISTPPEK
Sbjct: 781 VQMIVEEMNILTTIFLRYDNQKIVFPNSVLATKAIHNFYRSPDMGDAVEFCLHISTPPEK 840

Query: 841 IAVMRQRIVSYIEGKKEHWCSTPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAL 900
           IAVMRQRIVSYIE KKEHWC  PMIV KDVEELNR+RIAIWLTHRMNHQDMGERWTRRAL
Sbjct: 841 IAVMRQRIVSYIEAKKEHWCPAPMIVFKDVEELNRLRIAIWLTHRMNHQDMGERWTRRAL 900

Query: 901 LVEELVKIFQELDLQYRLLPLDINVRSLPPVNSTRLPATWTTTAS 935
           LVEEL+KIFQELDLQYRLLPLDINVR+LPPVNSTRLPATWT T S
Sbjct: 901 LVEELLKIFQELDLQYRLLPLDINVRTLPPVNSTRLPATWTATTS 942

BLAST of Tan0022520 vs. NCBI nr
Match: KAG6571002.1 (Mechanosensitive ion channel protein 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1611.3 bits (4171), Expect = 0.0e+00
Identity = 842/940 (89.57%), Postives = 893/940 (95.00%), Query Frame = 0

Query: 1   MDFSLKSFKSHGSYKYVRKLSG-----DQEQLPILSDQEPDNLHRNHSIMAPSDASDRRE 60
           MDFSLK+FKSHGSYKYVRKLSG     DQEQLPILSDQEP+NLH+  S MA SDASDR+E
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60

Query: 61  VIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFWNDSDGRRNNGEGSNGD-GRSEDF 120
           VIVK+D++DASSNAS+RAVESVNS+GRIWRESSYDFWNDSD RRNNGEGSNGD G+ EDF
Sbjct: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF 120

Query: 121 EFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPLDKTLPPVAESPMRRSSR 180
           EFRQ RKDVEDPPSKLIGQFLHKQKASGEM LDMDMEMLELP DKTLP VAESPMRRSS+
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK 180

Query: 181 ELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRRAYGNGHDDSGDEVVRCT 240
           ELKVSFESISEISE+DS+RRRHRDSP DEEHR     Q+ D R +GNGHDD   EVVRCT
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQHPPQR-DHRPHGNGHDDGAAEVVRCT 240

Query: 241 SNSSFQRSVSFQRKSSLLRAKTKSRLLDPPEQPDRRSGRVPKSGQMRSGLLSKALDEEDD 300
           SNSSF+R+VSFQRKSSLL+ KTKSRLLDPPE  DRRSGRVPKSGQ+RSGLLSKALDEEDD
Sbjct: 241 SNSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDD 300

Query: 301 DPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIPYWRQKNLWKLKLWKWEVM 360
           DPFLEEDLPDEYKKANLGVLT+LQWASLILIIAAL+CTLTI YWR+K LWKL+LWKWEVM
Sbjct: 301 DPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVM 360

Query: 361 ILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF 420
           ILVLICGRL SGWGIRIIVFFIER+FLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF
Sbjct: 361 ILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF 420

Query: 421 DDKVQREVKSNALEYVTKVLVCLLVTTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQ 480
           DDKVQREVKS+ALEYVTKVL+CLLV+TLVWLVKTL+VKVLASSFHVSTYFDRIQDAL+NQ
Sbjct: 421 DDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQ 480

Query: 481 YVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTPKGGRLIGSGG 540
           YVIETLSGPPLIEIQK++EEEE+LAEEV+KLQNAGATIPPDL+A+AFS+P+GGR+IGSGG
Sbjct: 481 YVIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRVIGSGG 540

Query: 541 LQRSPRGRSAKLSRVLSKKGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGALSTLDEQ 600
           LQ+SPRGRSAKLSR LSKKGDEGITIDHLH+LSPKNVSAWNMKRLMNIVRHGALSTLDEQ
Sbjct: 541 LQKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQ 600

Query: 601 IKDSTHEEDESATQIRSEFEAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEASKTMSL 660
           IKDS H EDES TQI+SE+EAKVAAKKIFQNVA  GSKYIYLEDLMRFM EDEASKTMSL
Sbjct: 601 IKDSAH-EDESTTQIKSEYEAKVAAKKIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSL 660

Query: 661 FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVILVIWL 720
           FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSV+ILVIWL
Sbjct: 661 FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWL 720

Query: 721 LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV 780
           LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV
Sbjct: 721 LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV 780

Query: 781 EEMNILTTIFLRYDNQKIIFPNSVLATKAIHNYYRSPDMGDSVEFCLHISTPPEKIAVMR 840
           EEMNILTT+FLR+DNQKIIFPNSVLATKAIHNYYRSPDMGD+VEFCLHISTPPEKIA+MR
Sbjct: 781 EEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMR 840

Query: 841 QRIVSYIEGKKEHWCSTPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEEL 900
           QRI+SYIEGKKEHWC  P+I+LKDVEELNRMRIAIWLTHRMNHQDMGERWTRRA LVEEL
Sbjct: 841 QRILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEEL 900

Query: 901 VKIFQELDLQYRLLPLDINVRSLPPVNSTRLPATWTTTAS 935
           VKIFQELDLQYRLLPL+INV SLPPVNSTRLPATW  TAS
Sbjct: 901 VKIFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of Tan0022520 vs. NCBI nr
Match: XP_023512518.1 (mechanosensitive ion channel protein 6-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512519.1 mechanosensitive ion channel protein 6-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1607.4 bits (4161), Expect = 0.0e+00
Identity = 839/940 (89.26%), Postives = 893/940 (95.00%), Query Frame = 0

Query: 1   MDFSLKSFKSHGSYKYVRKLSG-----DQEQLPILSDQEPDNLHRNHSIMAPSDASDRRE 60
           MDFSLK+FKSHGSYKYVRKLSG     DQEQLPILSDQEP+NLH+  S MA SDASDR+E
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKRSFMAVSDASDRKE 60

Query: 61  VIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFWNDSDGRRNNGEGSNGD-GRSEDF 120
           VIVK+D++DASSNAS+RAVESVNS+GRIWRESSYDFWNDSD +RNNGEGSNGD G+ EDF
Sbjct: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSKRNNGEGSNGDGGKGEDF 120

Query: 121 EFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPLDKTLPPVAESPMRRSSR 180
           EFRQ RKDVEDPPSKLIGQFLHKQKASGEM LDMDMEMLELP DKTLP VAESPMRRSS+
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPHDKTLPTVAESPMRRSSK 180

Query: 181 ELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRRAYGNGHDDSGDEVVRCT 240
           ELKVSFESISEISE+DS+RRRHRDSP DEEHR  Q  Q+ D R +GNGHDD   EVVRCT
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQR-DHRPHGNGHDDGAAEVVRCT 240

Query: 241 SNSSFQRSVSFQRKSSLLRAKTKSRLLDPPEQPDRRSGRVPKSGQMRSGLLSKALDEEDD 300
           SNSSF+R+VSFQRKSSLL+ KTKSRLLDPPE  DRRSGRVPKSGQ+RSGLLSKALDEEDD
Sbjct: 241 SNSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDD 300

Query: 301 DPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIPYWRQKNLWKLKLWKWEVM 360
           DPFLEEDLPDEYKKANLGVLT+LQWASLILIIAAL+CTLTI YWR+K LWKL+LWKWEVM
Sbjct: 301 DPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVM 360

Query: 361 ILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF 420
           ILVLICGRL SGWGIRIIVFFIER+FLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF
Sbjct: 361 ILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF 420

Query: 421 DDKVQREVKSNALEYVTKVLVCLLVTTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQ 480
           DDKVQREVKS+ALEYVTKVL+CLLV+TLVWLVKTL+VKVLASSFHVSTYFDRIQDAL+NQ
Sbjct: 421 DDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQ 480

Query: 481 YVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTPKGGRLIGSGG 540
           YVIETLSGPPLIEIQK++EEEE+LAEEV+KLQNAGATIPPDL+A+AFS+P+GGR+IGSGG
Sbjct: 481 YVIETLSGPPLIEIQKSKEEEEKLAEEVMKLQNAGATIPPDLRASAFSSPEGGRVIGSGG 540

Query: 541 LQRSPRGRSAKLSRVLSKKGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGALSTLDEQ 600
           LQ+SPRGRSAKLSR LSKKGDEGITIDHLH+LSPKNVSAWNMKRLMNIVRHGALSTLDEQ
Sbjct: 541 LQKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQ 600

Query: 601 IKDSTHEEDESATQIRSEFEAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEASKTMSL 660
           IKDS H EDES TQI+SE+EAKVAAK+IFQNVA  GSKYIYLEDLMRFM EDEASKTMSL
Sbjct: 601 IKDSAH-EDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSL 660

Query: 661 FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVILVIWL 720
           FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSV+ILVIWL
Sbjct: 661 FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWL 720

Query: 721 LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV 780
           LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV
Sbjct: 721 LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV 780

Query: 781 EEMNILTTIFLRYDNQKIIFPNSVLATKAIHNYYRSPDMGDSVEFCLHISTPPEKIAVMR 840
           EEMNILTT+FLR+DNQKIIFPNSVLATKAIHNYYRSPDMGD+VEFCLHISTPPEKIA+MR
Sbjct: 781 EEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMR 840

Query: 841 QRIVSYIEGKKEHWCSTPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEEL 900
           QRI+ +IEGKKEHWC  P+I+LKDVEELNRMRIAIWLTHRMNHQDMGERWTRRA LVEEL
Sbjct: 841 QRILGFIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEEL 900

Query: 901 VKIFQELDLQYRLLPLDINVRSLPPVNSTRLPATWTTTAS 935
           VKIFQELDLQYRLLPL+INV SLPPVNSTRLPATW  TAS
Sbjct: 901 VKIFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of Tan0022520 vs. NCBI nr
Match: XP_022943801.1 (mechanosensitive ion channel protein 6-like [Cucurbita moschata])

HSP 1 Score: 1606.7 bits (4159), Expect = 0.0e+00
Identity = 841/940 (89.47%), Postives = 890/940 (94.68%), Query Frame = 0

Query: 1   MDFSLKSFKSHGSYKYVRKLSG-----DQEQLPILSDQEPDNLHRNHSIMAPSDASDRRE 60
           MDFSLK+FKSHGSYKYVRKLSG     DQEQLPILSDQEP+NLH+  S MA SDASDR+E
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60

Query: 61  VIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFWNDSDGRRNNGEGSNGD-GRSEDF 120
           VIVK+D++DASSNAS+RAVESVNS+GRIWRESSYDFWNDSD RRNNGEGSNGD G+ EDF
Sbjct: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF 120

Query: 121 EFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPLDKTLPPVAESPMRRSSR 180
           EFRQ RKDVEDPPSKLIGQFLHKQKASGEM LDMDMEMLELP DKTLP VAESPMRRSS+
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK 180

Query: 181 ELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRRAYGNGHDDSGDEVVRCT 240
           ELKVSFESISEISE+DS+RRRHRDSP DEEHR  Q     D R +GNGHDD   EVV CT
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHR-VQHPPLRDHRPHGNGHDDGAAEVVSCT 240

Query: 241 SNSSFQRSVSFQRKSSLLRAKTKSRLLDPPEQPDRRSGRVPKSGQMRSGLLSKALDEEDD 300
           SNSSF+R+VSFQRKSSLL+ KTKSRLLDPPE  DRRSGRVPKSGQ+RSGLLSKALDEEDD
Sbjct: 241 SNSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDD 300

Query: 301 DPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIPYWRQKNLWKLKLWKWEVM 360
           DPFLEEDLPDEYKKANLGVLT+LQWASLILIIAAL+CTLTI YWR+K LWKL+LWKWEVM
Sbjct: 301 DPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVM 360

Query: 361 ILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF 420
           ILVLICGRL SGWGIRIIVFFIER+FLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF
Sbjct: 361 ILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF 420

Query: 421 DDKVQREVKSNALEYVTKVLVCLLVTTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQ 480
           DDKVQREVKS+ALEYVTKVL+CLLV+TLVWLVKTL+VKVLASSFHVSTYFDRIQDAL+NQ
Sbjct: 421 DDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQ 480

Query: 481 YVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTPKGGRLIGSGG 540
           YVIETLSGPPLIEIQKN+EEE +LAEEVIKLQNAGATIPPDL+A+AFS+P+GGR+IGSGG
Sbjct: 481 YVIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGG 540

Query: 541 LQRSPRGRSAKLSRVLSKKGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGALSTLDEQ 600
           LQ+SPRGRSAKLSR LSKKGDEGITIDHLH+LSPKNVSAWNMKRLMNIVRHGALSTLDEQ
Sbjct: 541 LQKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQ 600

Query: 601 IKDSTHEEDESATQIRSEFEAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEASKTMSL 660
           IKDS H EDES TQI+SE+EAKVAAK+IFQNVA  GSKYIYLEDLMRFM EDEASKTMSL
Sbjct: 601 IKDSAH-EDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSL 660

Query: 661 FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVILVIWL 720
           FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSV+ILVIWL
Sbjct: 661 FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWL 720

Query: 721 LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV 780
           LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV
Sbjct: 721 LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV 780

Query: 781 EEMNILTTIFLRYDNQKIIFPNSVLATKAIHNYYRSPDMGDSVEFCLHISTPPEKIAVMR 840
           EEMNILTT+FLR+DNQKIIFPNSVLATKAIHNYYRSPDMGD+VEFCLHISTPPEKIA+MR
Sbjct: 781 EEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMR 840

Query: 841 QRIVSYIEGKKEHWCSTPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEEL 900
           QRI+SYIEGKKEHWC  P+I+LKDVEELNRMRIAIWLTHRMNHQDMGERWTRRA LVEEL
Sbjct: 841 QRILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEEL 900

Query: 901 VKIFQELDLQYRLLPLDINVRSLPPVNSTRLPATWTTTAS 935
           VKIFQELDLQYRLLPL+INV SLPPVNSTRLPATW  TAS
Sbjct: 901 VKIFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of Tan0022520 vs. NCBI nr
Match: XP_022985632.1 (mechanosensitive ion channel protein 6-like [Cucurbita maxima])

HSP 1 Score: 1600.9 bits (4144), Expect = 0.0e+00
Identity = 838/940 (89.15%), Postives = 888/940 (94.47%), Query Frame = 0

Query: 1   MDFSLKSFKSHGSYKYVRKLSG-----DQEQLPILSDQEPDNLHRNHSIMAPSDASDRRE 60
           MDFSLK+FKSHGSYKYVRKLSG     DQEQLPILSDQEP+NLH+  S MA SDASDR+E
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDTTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60

Query: 61  VIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFWNDSDGRRNNGEGSNGD-GRSEDF 120
           VIVK+DQ+D SSNAS+R VESVNS+GRIWRESSYDFWNDS  RRNNGEGS+GD G+ EDF
Sbjct: 61  VIVKVDQADVSSNASARPVESVNSSGRIWRESSYDFWNDSGRRRNNGEGSDGDGGKGEDF 120

Query: 121 EFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPLDKTLPPVAESPMRRSSR 180
           EFRQ RKDVEDPPSKLIGQFLHKQKASGEM LDMDMEMLELP DKTLPPV ESP+RRSS+
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPPVVESPLRRSSK 180

Query: 181 ELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRRAYGNGHDDSGDEVVRCT 240
           ELKVSFESISEISE+DS+RRRHRDSP DEEHR  Q  Q+ D RA+GNGHDD   EVVRCT
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQR-DHRAHGNGHDDGTPEVVRCT 240

Query: 241 SNSSFQRSVSFQRKSSLLRAKTKSRLLDPPEQPDRRSGRVPKSGQMRSGLLSKALDEEDD 300
           SNSSF+R VSFQRKSSLL+ KTKSRLLDPPE  DRRSGRVPKSG++RSGLLSKALDEEDD
Sbjct: 241 SNSSFERGVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGKIRSGLLSKALDEEDD 300

Query: 301 DPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIPYWRQKNLWKLKLWKWEVM 360
           DPFLEEDLPDEYKKANLGVLT+LQWASLILIIAAL+CTLTI YWR+K LWKL+LWKWEVM
Sbjct: 301 DPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVM 360

Query: 361 ILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF 420
           ILVLICGRL SGWGIRIIVF IER+FLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF
Sbjct: 361 ILVLICGRLFSGWGIRIIVFLIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF 420

Query: 421 DDKVQREVKSNALEYVTKVLVCLLVTTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQ 480
           DDKVQREVKS+ALEYVTKVL+CLLV+TLVWLVKTL+VKVLASSFHVSTYFDRIQDAL+NQ
Sbjct: 421 DDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQ 480

Query: 481 YVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTPKGGRLIGSGG 540
           YVIETLSGPPLIEIQKN+EEE +LAEEVIKLQNAGATIPPDL+A+AFS+P+GGR+IGSGG
Sbjct: 481 YVIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGG 540

Query: 541 LQRSPRGRSAKLSRVLSKKGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGALSTLDEQ 600
           LQ+SPRGRSAKLSR LSKKGDEGITIDHLH+LSPKNVSAWNMKRLMNIVRHGALSTLDEQ
Sbjct: 541 LQKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQ 600

Query: 601 IKDSTHEEDESATQIRSEFEAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEASKTMSL 660
           IKDS H EDES TQI+SE+EAKVAAKKIFQNVA  GSKYIYLEDLMRFM EDEASKTMSL
Sbjct: 601 IKDSAH-EDESTTQIKSEYEAKVAAKKIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSL 660

Query: 661 FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVILVIWL 720
           FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSV+ILVIWL
Sbjct: 661 FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWL 720

Query: 721 LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV 780
           LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV
Sbjct: 721 LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV 780

Query: 781 EEMNILTTIFLRYDNQKIIFPNSVLATKAIHNYYRSPDMGDSVEFCLHISTPPEKIAVMR 840
           EEMNILTT+FLR+DNQKIIFPNSVLATKAIHNYYRSPDMGD+VEFCLHISTPPEKIA+MR
Sbjct: 781 EEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMR 840

Query: 841 QRIVSYIEGKKEHWCSTPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEEL 900
           QRI+SYIE KKEHWC  P+I+LKDVEELNRMRIAIWLTHRMNHQDMGERWTRRA LVEEL
Sbjct: 841 QRILSYIEDKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEEL 900

Query: 901 VKIFQELDLQYRLLPLDINVRSLPPVNSTRLPATWTTTAS 935
           VKIFQELDLQYRLLPL+INV SLPPVNSTRLPATW  TAS
Sbjct: 901 VKIFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of Tan0022520 vs. ExPASy TrEMBL
Match: A0A6J1CFM7 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111011094 PE=3 SV=1)

HSP 1 Score: 1632.8 bits (4227), Expect = 0.0e+00
Identity = 865/945 (91.53%), Postives = 896/945 (94.81%), Query Frame = 0

Query: 1   MDFSLKSFKSHGSYKYVRKLSG------DQEQLPILSDQEPDNLHRNHSIMAPSDASDRR 60
           MDFSLKSFKSHGSYKY RKLSG       QEQLPILSDQ+P NLH+  S MA SDASDR 
Sbjct: 1   MDFSLKSFKSHGSYKYARKLSGGGGADDSQEQLPILSDQDPGNLHQQQS-MAVSDASDRN 60

Query: 61  EVIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFWNDSDGRRNNGEGSNGDGRSEDF 120
           EVIVKIDQ D S NA+SR +E+VN++GRIWRESSYDFWND DGRR+N EGSNGDGRSEDF
Sbjct: 61  EVIVKIDQPD-SLNATSRVMEAVNASGRIWRESSYDFWNDGDGRRSNAEGSNGDGRSEDF 120

Query: 121 EFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPLDKTLPPVAESPMRRSSR 180
           EFRQHR+ VEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELP DKTLPPVAESPMRRSS+
Sbjct: 121 EFRQHRQGVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTLPPVAESPMRRSSK 180

Query: 181 ELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRRAYGNGHDDSGDEVVRCT 240
           ELKVSFESISEISENDS+RRRHRDSPADEE++G Q  Q+ DRRAYGNGHDD G EVVRCT
Sbjct: 181 ELKVSFESISEISENDSMRRRHRDSPADEEYKGQQPPQR-DRRAYGNGHDDGGAEVVRCT 240

Query: 241 SNSSFQRSVSFQRKSSLLRAKTKSRLLDPPEQPDR----RSGRV-PKSGQMRSGLLSKAL 300
           SNSSFQR VSFQRKSSLLRAKTKSRLLDPPEQPDR    RSGRV PKSGQ+RSGLLSKAL
Sbjct: 241 SNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEQPDRRSGVRSGRVPPKSGQIRSGLLSKAL 300

Query: 301 DEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIPYWRQKNLWKLKLW 360
           DEEDDDPFLEEDLPDEYKK+NLG LTLLQWASLILIIAALVCTL+IPY  +KNLWKLKLW
Sbjct: 301 DEEDDDPFLEEDLPDEYKKSNLGALTLLQWASLILIIAALVCTLSIPYLNRKNLWKLKLW 360

Query: 361 KWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIA 420
           KWEVM+LVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIA
Sbjct: 361 KWEVMVLVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIA 420

Query: 421 WNFLFDDKVQREVKSNALEYVTKVLVCLLVTTLVWLVKTLMVKVLASSFHVSTYFDRIQD 480
           WNFLFDDKVQREVKSNALEYVTKVLVCLLV+TLVWLVKTLMVKVLASSFHVSTYFDRIQD
Sbjct: 421 WNFLFDDKVQREVKSNALEYVTKVLVCLLVSTLVWLVKTLMVKVLASSFHVSTYFDRIQD 480

Query: 481 ALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTPKGGRL 540
           ALFNQYVIETLSGPPLIEI+KNEEEEERLAEEV+KLQNAGATIPPDLKATAFST KGGR+
Sbjct: 481 ALFNQYVIETLSGPPLIEIRKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAKGGRV 540

Query: 541 IGSGGLQRSPRGRSAKLSRVLSKKGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGALS 600
           IGSGGLQRSPRGRS KLSR LSK GD+GITIDHLHKLSPKNVSAWNMKRLMNIVRHGALS
Sbjct: 541 IGSGGLQRSPRGRSGKLSRSLSKNGDDGITIDHLHKLSPKNVSAWNMKRLMNIVRHGALS 600

Query: 601 TLDEQIKDSTHEEDESATQIRSEFEAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEAS 660
           TLDEQIKDSTHEEDESATQI+SE EAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEA 
Sbjct: 601 TLDEQIKDSTHEEDESATQIKSECEAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEAL 660

Query: 661 KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVI 720
           KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVI
Sbjct: 661 KTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVI 720

Query: 721 LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG 780
           LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG
Sbjct: 721 LVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDG 780

Query: 781 VQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNYYRSPDMGDSVEFCLHISTPPEK 840
           VQMIVEEMNILTTIFLRYDNQKI+FPNSVLATKAIHN+YRSPDMGD+VEFCLHISTPPEK
Sbjct: 781 VQMIVEEMNILTTIFLRYDNQKIVFPNSVLATKAIHNFYRSPDMGDAVEFCLHISTPPEK 840

Query: 841 IAVMRQRIVSYIEGKKEHWCSTPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAL 900
           IAVMRQRIVSYIE KKEHWC  PMIV KDVEELNR+RIAIWLTHRMNHQDMGERWTRRAL
Sbjct: 841 IAVMRQRIVSYIEAKKEHWCPAPMIVFKDVEELNRLRIAIWLTHRMNHQDMGERWTRRAL 900

Query: 901 LVEELVKIFQELDLQYRLLPLDINVRSLPPVNSTRLPATWTTTAS 935
           LVEEL+KIFQELDLQYRLLPLDINVR+LPPVNSTRLPATWT T S
Sbjct: 901 LVEELLKIFQELDLQYRLLPLDINVRTLPPVNSTRLPATWTATTS 942

BLAST of Tan0022520 vs. ExPASy TrEMBL
Match: A0A6J1FSP8 (Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC111448441 PE=3 SV=1)

HSP 1 Score: 1606.7 bits (4159), Expect = 0.0e+00
Identity = 841/940 (89.47%), Postives = 890/940 (94.68%), Query Frame = 0

Query: 1   MDFSLKSFKSHGSYKYVRKLSG-----DQEQLPILSDQEPDNLHRNHSIMAPSDASDRRE 60
           MDFSLK+FKSHGSYKYVRKLSG     DQEQLPILSDQEP+NLH+  S MA SDASDR+E
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDSTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60

Query: 61  VIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFWNDSDGRRNNGEGSNGD-GRSEDF 120
           VIVK+D++DASSNAS+RAVESVNS+GRIWRESSYDFWNDSD RRNNGEGSNGD G+ EDF
Sbjct: 61  VIVKVDEADASSNASARAVESVNSSGRIWRESSYDFWNDSDSRRNNGEGSNGDGGKGEDF 120

Query: 121 EFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPLDKTLPPVAESPMRRSSR 180
           EFRQ RKDVEDPPSKLIGQFLHKQKASGEM LDMDMEMLELP DKTLP VAESPMRRSS+
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPTVAESPMRRSSK 180

Query: 181 ELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRRAYGNGHDDSGDEVVRCT 240
           ELKVSFESISEISE+DS+RRRHRDSP DEEHR  Q     D R +GNGHDD   EVV CT
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHR-VQHPPLRDHRPHGNGHDDGAAEVVSCT 240

Query: 241 SNSSFQRSVSFQRKSSLLRAKTKSRLLDPPEQPDRRSGRVPKSGQMRSGLLSKALDEEDD 300
           SNSSF+R+VSFQRKSSLL+ KTKSRLLDPPE  DRRSGRVPKSGQ+RSGLLSKALDEEDD
Sbjct: 241 SNSSFERNVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGQIRSGLLSKALDEEDD 300

Query: 301 DPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIPYWRQKNLWKLKLWKWEVM 360
           DPFLEEDLPDEYKKANLGVLT+LQWASLILIIAAL+CTLTI YWR+K LWKL+LWKWEVM
Sbjct: 301 DPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVM 360

Query: 361 ILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF 420
           ILVLICGRL SGWGIRIIVFFIER+FLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF
Sbjct: 361 ILVLICGRLFSGWGIRIIVFFIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF 420

Query: 421 DDKVQREVKSNALEYVTKVLVCLLVTTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQ 480
           DDKVQREVKS+ALEYVTKVL+CLLV+TLVWLVKTL+VKVLASSFHVSTYFDRIQDAL+NQ
Sbjct: 421 DDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQ 480

Query: 481 YVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTPKGGRLIGSGG 540
           YVIETLSGPPLIEIQKN+EEE +LAEEVIKLQNAGATIPPDL+A+AFS+P+GGR+IGSGG
Sbjct: 481 YVIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGG 540

Query: 541 LQRSPRGRSAKLSRVLSKKGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGALSTLDEQ 600
           LQ+SPRGRSAKLSR LSKKGDEGITIDHLH+LSPKNVSAWNMKRLMNIVRHGALSTLDEQ
Sbjct: 541 LQKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQ 600

Query: 601 IKDSTHEEDESATQIRSEFEAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEASKTMSL 660
           IKDS H EDES TQI+SE+EAKVAAK+IFQNVA  GSKYIYLEDLMRFM EDEASKTMSL
Sbjct: 601 IKDSAH-EDESTTQIKSEYEAKVAAKRIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSL 660

Query: 661 FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVILVIWL 720
           FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSV+ILVIWL
Sbjct: 661 FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWL 720

Query: 721 LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV 780
           LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV
Sbjct: 721 LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV 780

Query: 781 EEMNILTTIFLRYDNQKIIFPNSVLATKAIHNYYRSPDMGDSVEFCLHISTPPEKIAVMR 840
           EEMNILTT+FLR+DNQKIIFPNSVLATKAIHNYYRSPDMGD+VEFCLHISTPPEKIA+MR
Sbjct: 781 EEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMR 840

Query: 841 QRIVSYIEGKKEHWCSTPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEEL 900
           QRI+SYIEGKKEHWC  P+I+LKDVEELNRMRIAIWLTHRMNHQDMGERWTRRA LVEEL
Sbjct: 841 QRILSYIEGKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEEL 900

Query: 901 VKIFQELDLQYRLLPLDINVRSLPPVNSTRLPATWTTTAS 935
           VKIFQELDLQYRLLPL+INV SLPPVNSTRLPATW  TAS
Sbjct: 901 VKIFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of Tan0022520 vs. ExPASy TrEMBL
Match: A0A6J1J5E6 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111483636 PE=3 SV=1)

HSP 1 Score: 1600.9 bits (4144), Expect = 0.0e+00
Identity = 838/940 (89.15%), Postives = 888/940 (94.47%), Query Frame = 0

Query: 1   MDFSLKSFKSHGSYKYVRKLSG-----DQEQLPILSDQEPDNLHRNHSIMAPSDASDRRE 60
           MDFSLK+FKSHGSYKYVRKLSG     DQEQLPILSDQEP+NLH+  S MA SDASDR+E
Sbjct: 1   MDFSLKAFKSHGSYKYVRKLSGTDTTQDQEQLPILSDQEPNNLHQKQSFMAVSDASDRKE 60

Query: 61  VIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFWNDSDGRRNNGEGSNGD-GRSEDF 120
           VIVK+DQ+D SSNAS+R VESVNS+GRIWRESSYDFWNDS  RRNNGEGS+GD G+ EDF
Sbjct: 61  VIVKVDQADVSSNASARPVESVNSSGRIWRESSYDFWNDSGRRRNNGEGSDGDGGKGEDF 120

Query: 121 EFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPLDKTLPPVAESPMRRSSR 180
           EFRQ RKDVEDPPSKLIGQFLHKQKASGEM LDMDMEMLELP DKTLPPV ESP+RRSS+
Sbjct: 121 EFRQQRKDVEDPPSKLIGQFLHKQKASGEMCLDMDMEMLELPQDKTLPPVVESPLRRSSK 180

Query: 181 ELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRRAYGNGHDDSGDEVVRCT 240
           ELKVSFESISEISE+DS+RRRHRDSP DEEHR  Q  Q+ D RA+GNGHDD   EVVRCT
Sbjct: 181 ELKVSFESISEISESDSLRRRHRDSPVDEEHRVQQPPQR-DHRAHGNGHDDGTPEVVRCT 240

Query: 241 SNSSFQRSVSFQRKSSLLRAKTKSRLLDPPEQPDRRSGRVPKSGQMRSGLLSKALDEEDD 300
           SNSSF+R VSFQRKSSLL+ KTKSRLLDPPE  DRRSGRVPKSG++RSGLLSKALDEEDD
Sbjct: 241 SNSSFERGVSFQRKSSLLKTKTKSRLLDPPEHQDRRSGRVPKSGKIRSGLLSKALDEEDD 300

Query: 301 DPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIPYWRQKNLWKLKLWKWEVM 360
           DPFLEEDLPDEYKKANLGVLT+LQWASLILIIAAL+CTLTI YWR+K LWKL+LWKWEVM
Sbjct: 301 DPFLEEDLPDEYKKANLGVLTMLQWASLILIIAALICTLTIRYWRRKKLWKLELWKWEVM 360

Query: 361 ILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF 420
           ILVLICGRL SGWGIRIIVF IER+FLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF
Sbjct: 361 ILVLICGRLFSGWGIRIIVFLIERSFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLF 420

Query: 421 DDKVQREVKSNALEYVTKVLVCLLVTTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQ 480
           DDKVQREVKS+ALEYVTKVL+CLLV+TLVWLVKTL+VKVLASSFHVSTYFDRIQDAL+NQ
Sbjct: 421 DDKVQREVKSDALEYVTKVLICLLVSTLVWLVKTLIVKVLASSFHVSTYFDRIQDALYNQ 480

Query: 481 YVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFSTPKGGRLIGSGG 540
           YVIETLSGPPLIEIQKN+EEE +LAEEVIKLQNAGATIPPDL+A+AFS+P+GGR+IGSGG
Sbjct: 481 YVIETLSGPPLIEIQKNKEEEVKLAEEVIKLQNAGATIPPDLRASAFSSPEGGRVIGSGG 540

Query: 541 LQRSPRGRSAKLSRVLSKKGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGALSTLDEQ 600
           LQ+SPRGRSAKLSR LSKKGDEGITIDHLH+LSPKNVSAWNMKRLMNIVRHGALSTLDEQ
Sbjct: 541 LQKSPRGRSAKLSRALSKKGDEGITIDHLHRLSPKNVSAWNMKRLMNIVRHGALSTLDEQ 600

Query: 601 IKDSTHEEDESATQIRSEFEAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEASKTMSL 660
           IKDS H EDES TQI+SE+EAKVAAKKIFQNVA  GSKYIYLEDLMRFM EDEASKTMSL
Sbjct: 601 IKDSAH-EDESTTQIKSEYEAKVAAKKIFQNVASQGSKYIYLEDLMRFMVEDEASKTMSL 660

Query: 661 FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVILVIWL 720
           FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSV+ILVIWL
Sbjct: 661 FEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILVIWL 720

Query: 721 LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV 780
           LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV
Sbjct: 721 LILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIV 780

Query: 781 EEMNILTTIFLRYDNQKIIFPNSVLATKAIHNYYRSPDMGDSVEFCLHISTPPEKIAVMR 840
           EEMNILTT+FLR+DNQKIIFPNSVLATKAIHNYYRSPDMGD+VEFCLHISTPPEKIA+MR
Sbjct: 781 EEMNILTTVFLRFDNQKIIFPNSVLATKAIHNYYRSPDMGDAVEFCLHISTPPEKIAIMR 840

Query: 841 QRIVSYIEGKKEHWCSTPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEEL 900
           QRI+SYIE KKEHWC  P+I+LKDVEELNRMRIAIWLTHRMNHQDMGERWTRRA LVEEL
Sbjct: 841 QRILSYIEDKKEHWCPAPLIILKDVEELNRMRIAIWLTHRMNHQDMGERWTRRAFLVEEL 900

Query: 901 VKIFQELDLQYRLLPLDINVRSLPPVNSTRLPATWTTTAS 935
           VKIFQELDLQYRLLPL+INV SLPPVNSTRLPATW  TAS
Sbjct: 901 VKIFQELDLQYRLLPLNINVCSLPPVNSTRLPATWGATAS 938

BLAST of Tan0022520 vs. ExPASy TrEMBL
Match: A0A5D3DK11 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold250G00230 PE=3 SV=1)

HSP 1 Score: 1557.0 bits (4030), Expect = 0.0e+00
Identity = 835/935 (89.30%), Postives = 870/935 (93.05%), Query Frame = 0

Query: 1   MDFSLKSFKSHGSYKYVRKLSG-----DQEQLPILSDQEPDNLHRNHSIMAPSDASDRRE 60
           MDFSLKSFKSH SYKYVRKLSG     DQE LPILSD +       HSIMA SDASDR+E
Sbjct: 1   MDFSLKSFKSHPSYKYVRKLSGADATQDQEHLPILSDHQ-------HSIMAVSDASDRKE 60

Query: 61  VIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFWNDSDGRRNNGEGSNGDGRSEDFE 120
           VIVK+D++DAS   S RA + VN +GRIWRESSYDFWNDSD RRNNGEG  G   +EDFE
Sbjct: 61  VIVKVDEADAS---SLRATDLVNGSGRIWRESSYDFWNDSDNRRNNGEG--GARTTEDFE 120

Query: 121 FRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPLDKT-LPPVAESPMRRSSR 180
           FRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELP DKT L  VAESPMRRSSR
Sbjct: 121 FRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSR 180

Query: 181 ELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRRAYG-NGHDDSGDEVVRC 240
           ELKVSFESISEISENDS+RRRHRDSP DEEHR GQQ ++ DRRA+G NG DD   EV+RC
Sbjct: 181 ELKVSFESISEISENDSMRRRHRDSPLDEEHR-GQQPRQCDRRAHGSNGEDDGAAEVLRC 240

Query: 241 TSNSSFQRSVSFQRKSSLLRAKTKSRLLDPPEQPDRRSGRVPKSGQMRSGLLSKALDEED 300
           +SNSSFQR VSFQRKSSLLRAKTKSRLLDPPE  DRRSGRVPKSGQ+RSGL+SK LDEED
Sbjct: 241 SSNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKVLDEED 300

Query: 301 DDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIPYWRQKNLWKLKLWKWEV 360
           DDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTI YWR+K LWKL++WKWEV
Sbjct: 301 DDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEV 360

Query: 361 MILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL 420
           MILVLICGRLVSGWGIR+IVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL
Sbjct: 361 MILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL 420

Query: 421 FDDKVQREVKSNALEYVTKVLVCLLVTTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFN 480
           FDDKVQREVKSNALEYVTKVLVCLL++TLVWLVKTLMVKVLASSFHVSTYFDRIQDALFN
Sbjct: 421 FDDKVQREVKSNALEYVTKVLVCLLISTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFN 480

Query: 481 QYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFST-PKGGRLIGS 540
           QYVIETLSGPPLIEIQKNEEEEERLAEEV+KLQNAGATIPPDLKATAFST  KGGR+IGS
Sbjct: 481 QYVIETLSGPPLIEIQKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAQKGGRVIGS 540

Query: 541 GGLQRSPRGRSAKLSRVLSKK-GDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGALSTL 600
           GGLQ+SPRGRS KLSR LSKK GDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHG LSTL
Sbjct: 541 GGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTL 600

Query: 601 DEQIKDSTHEEDESATQIRSEFEAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEASKT 660
           DEQIKD+ H EDES TQI+SE+EAKVAAKKIF NVAR+GSKYIYLEDLMRFM++DEASKT
Sbjct: 601 DEQIKDTAH-EDESTTQIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKT 660

Query: 661 MSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVILV 720
           M LFEGA ESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSV+ILV
Sbjct: 661 MGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILV 720

Query: 721 IWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQ 780
           IWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFE+IIFLFVMHPFDVGDRCEIDGVQ
Sbjct: 721 IWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQ 780

Query: 781 MIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNYYRSPDMGDSVEFCLHISTPPEKIA 840
           MIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHN+YRSPDMGD VEFCLHISTPPEKIA
Sbjct: 781 MIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGVEFCLHISTPPEKIA 840

Query: 841 VMRQRIVSYIEGKKEHWCSTPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLV 900
           +MRQRIVSYIEGKKEHWC  PMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLV
Sbjct: 841 IMRQRIVSYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLV 900

Query: 901 EELVKIFQELDLQYRLLPLDINVRSLPPVNSTRLP 927
           EELVKIFQELDLQYRLLPLDINVRSLPPVNST  P
Sbjct: 901 EELVKIFQELDLQYRLLPLDINVRSLPPVNSTNFP 921

BLAST of Tan0022520 vs. ExPASy TrEMBL
Match: A0A1S3BIW1 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103490566 PE=3 SV=1)

HSP 1 Score: 1557.0 bits (4030), Expect = 0.0e+00
Identity = 835/935 (89.30%), Postives = 870/935 (93.05%), Query Frame = 0

Query: 1   MDFSLKSFKSHGSYKYVRKLSG-----DQEQLPILSDQEPDNLHRNHSIMAPSDASDRRE 60
           MDFSLKSFKSH SYKYVRKLSG     DQE LPILSD +       HSIMA SDASDR+E
Sbjct: 1   MDFSLKSFKSHPSYKYVRKLSGADATQDQEHLPILSDHQ-------HSIMAVSDASDRKE 60

Query: 61  VIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFWNDSDGRRNNGEGSNGDGRSEDFE 120
           VIVK+D++DAS   S RA + VN +GRIWRESSYDFWNDSD RRNNGEG  G   +EDFE
Sbjct: 61  VIVKVDEADAS---SLRATDLVNGSGRIWRESSYDFWNDSDNRRNNGEG--GARTTEDFE 120

Query: 121 FRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPLDKT-LPPVAESPMRRSSR 180
           FRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELP DKT L  VAESPMRRSSR
Sbjct: 121 FRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPQDKTPLSTVAESPMRRSSR 180

Query: 181 ELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRRAYG-NGHDDSGDEVVRC 240
           ELKVSFESISEISENDS+RRRHRDSP DEEHR GQQ ++ DRRA+G NG DD   EV+RC
Sbjct: 181 ELKVSFESISEISENDSMRRRHRDSPLDEEHR-GQQPRQCDRRAHGSNGEDDGAAEVLRC 240

Query: 241 TSNSSFQRSVSFQRKSSLLRAKTKSRLLDPPEQPDRRSGRVPKSGQMRSGLLSKALDEED 300
           +SNSSFQR VSFQRKSSLLRAKTKSRLLDPPE  DRRSGRVPKSGQ+RSGL+SK LDEED
Sbjct: 241 SSNSSFQRDVSFQRKSSLLRAKTKSRLLDPPEHQDRRSGRVPKSGQVRSGLISKVLDEED 300

Query: 301 DDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIPYWRQKNLWKLKLWKWEV 360
           DDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTI YWR+K LWKL++WKWEV
Sbjct: 301 DDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIRYWRRKKLWKLEVWKWEV 360

Query: 361 MILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL 420
           MILVLICGRLVSGWGIR+IVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL
Sbjct: 361 MILVLICGRLVSGWGIRVIVFFIERNFLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFL 420

Query: 421 FDDKVQREVKSNALEYVTKVLVCLLVTTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFN 480
           FDDKVQREVKSNALEYVTKVLVCLL++TLVWLVKTLMVKVLASSFHVSTYFDRIQDALFN
Sbjct: 421 FDDKVQREVKSNALEYVTKVLVCLLISTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFN 480

Query: 481 QYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATAFST-PKGGRLIGS 540
           QYVIETLSGPPLIEIQKNEEEEERLAEEV+KLQNAGATIPPDLKATAFST  KGGR+IGS
Sbjct: 481 QYVIETLSGPPLIEIQKNEEEEERLAEEVMKLQNAGATIPPDLKATAFSTAQKGGRVIGS 540

Query: 541 GGLQRSPRGRSAKLSRVLSKK-GDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGALSTL 600
           GGLQ+SPRGRS KLSR LSKK GDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHG LSTL
Sbjct: 541 GGLQKSPRGRSGKLSRTLSKKGGDEGITIDHLHKLSPKNVSAWNMKRLMNIVRHGTLSTL 600

Query: 601 DEQIKDSTHEEDESATQIRSEFEAKVAAKKIFQNVARHGSKYIYLEDLMRFMQEDEASKT 660
           DEQIKD+ H EDES TQI+SE+EAKVAAKKIF NVAR+GSKYIYLEDLMRFM++DEASKT
Sbjct: 601 DEQIKDTAH-EDESTTQIKSEYEAKVAAKKIFLNVARNGSKYIYLEDLMRFMEKDEASKT 660

Query: 661 MSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVVILV 720
           M LFEGA ESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSV+ILV
Sbjct: 661 MGLFEGACESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHRMVNILVSVIILV 720

Query: 721 IWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQ 780
           IWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFE+IIFLFVMHPFDVGDRCEIDGVQ
Sbjct: 721 IWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFESIIFLFVMHPFDVGDRCEIDGVQ 780

Query: 781 MIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNYYRSPDMGDSVEFCLHISTPPEKIA 840
           MIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHN+YRSPDMGD VEFCLHISTPPEKIA
Sbjct: 781 MIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNFYRSPDMGDGVEFCLHISTPPEKIA 840

Query: 841 VMRQRIVSYIEGKKEHWCSTPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLV 900
           +MRQRIVSYIEGKKEHWC  PMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLV
Sbjct: 841 IMRQRIVSYIEGKKEHWCPAPMIVLKDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLV 900

Query: 901 EELVKIFQELDLQYRLLPLDINVRSLPPVNSTRLP 927
           EELVKIFQELDLQYRLLPLDINVRSLPPVNST  P
Sbjct: 901 EELVKIFQELDLQYRLLPLDINVRSLPPVNSTNFP 921

BLAST of Tan0022520 vs. TAIR 10
Match: AT1G78610.1 (mechanosensitive channel of small conductance-like 6 )

HSP 1 Score: 972.6 bits (2513), Expect = 2.2e-283
Identity = 552/921 (59.93%), Postives = 683/921 (74.16%), Query Frame = 0

Query: 49  DASDRREVIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFWNDSDGRRNNGEG---- 108
           DA+DRREVIVKID  + ++N  S   E+V   G+IWR+ SYDFW D +G  N G      
Sbjct: 4   DAADRREVIVKIDGENGNNNGVSG--ETV---GKIWRDGSYDFWTDGEGNLNKGHNAAAV 63

Query: 109 --------SNGDGRSEDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELP 168
                   +    + E FEFR+     EDPP+KLIGQFLHKQ+ASGE+ LDMD+ M EL 
Sbjct: 64  DSDRSAATTGEQQKDEGFEFRRG----EDPPTKLIGQFLHKQQASGEICLDMDLGMDELQ 123

Query: 169 LDKTLPPVAESPMRRSSRELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDR 228
             + L PV+ESP        +VS       ++ D + RR                   D 
Sbjct: 124 -SRGLTPVSESP--------RVS-------TKRDPVGRR-------------------DS 183

Query: 229 RAYGNGHDDSGDEVVRCTSNSS-FQRSVSFQRKSSLLRAKTKSRLLD------PPEQPDR 288
           R+  N +DD   EVV+C+ N++  QRS      S+LL+ +T+SRL D      PP+  D 
Sbjct: 184 RSNTNNNDDG--EVVKCSGNNAPIQRS-----SSTLLKMRTRSRLSDPPTPQLPPQTADM 243

Query: 289 RSGRVPKSGQMRSGLLSKAL----DEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILI 348
           +SGR+PKSGQM+SG   K+     +EE+DDPF  EDLP+EY+K  L +  +L+W SLILI
Sbjct: 244 KSGRIPKSGQMKSGFFGKSPKTQGEEEEDDPFAAEDLPEEYRKDKLSLWIVLEWLSLILI 303

Query: 349 IAALVCTLTIPYWRQKNLWKLKLWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKR 408
           IA  VCTL IP  R+K LW+L+LWKWE M+LVLICGRLVS W ++I+VFFIERNFLLRKR
Sbjct: 304 IAGFVCTLAIPSLRKKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKR 363

Query: 409 VLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVTTLVWL 468
           VLYFVYGVRKAVQNCLWLGLVL+AW+FLFD+KV +   + AL  VTK+ VCLLV  L+WL
Sbjct: 364 VLYFVYGVRKAVQNCLWLGLVLLAWHFLFDEKVAKAANTKALRVVTKIFVCLLVGFLLWL 423

Query: 469 VKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKL 528
           VKTL+VKVLASSFH+STYFDRIQ++LF QYVIETLSGPPLIEIQKNEEEEER++ EV K 
Sbjct: 424 VKTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKF 483

Query: 529 QNAGATIPPDLKATAFSTPKGGRLIGSGGLQRSP--RGRSAKLSRVLSKKG-----DEGI 588
           QN                  GG  I SG  Q+SP   G+S  LS VLS  G     ++GI
Sbjct: 484 QN-----------------PGGVEIQSGA-QKSPMKTGKSPFLSHVLSNGGGGGGENKGI 543

Query: 589 TIDHLHKLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSTHEEDESATQIRSEFEAKVA 648
           TID LHKL+PKNVSAW MKRLMNI+R+G+L+TLDEQ++D + ++D+   QIRSEFEAK+A
Sbjct: 544 TIDSLHKLNPKNVSAWKMKRLMNIIRNGSLTTLDEQLQDPSLDDDK-GNQIRSEFEAKLA 603

Query: 649 AKKIFQNVARHGSKYIYLEDLMRFMQEDEASKTMSLFEGASESRKISKSSLKNWVVNAFR 708
           A+KIF NVA+ GSK+IY  D+MRF+ +DEA KT+SLFEGASE+ +ISKSSLKNWVVNAFR
Sbjct: 604 ARKIFHNVAKPGSKFIYANDIMRFLPDDEALKTLSLFEGASETNRISKSSLKNWVVNAFR 663

Query: 709 ERRALALTLNDTKTAVNKLHRMVNILVSVVILVIWLLILGIATSKFLLFVTSQLVLVAFV 768
           ERRALALTLNDTKTAVN+LH+MVNI+V ++ILVIWL+ILGI ++KFL+ ++SQ+V+VAF+
Sbjct: 664 ERRALALTLNDTKTAVNRLHKMVNIVVGIIILVIWLIILGITSTKFLVVMSSQVVVVAFI 723

Query: 769 FGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSV 828
           FGN CK VFE+II+LFV+HPFDVGDRCEIDGVQM+VEEMNILTT+FLR+DNQK+++PNS+
Sbjct: 724 FGNMCKIVFESIIYLFVIHPFDVGDRCEIDGVQMVVEEMNILTTVFLRFDNQKVVYPNSL 783

Query: 829 LATKAIHNYYRSPDMGDSVEFCLHISTPPEKIAVMRQRIVSYIEGKKEHWCSTPMIVLKD 888
           L TK+I NYYRSPDMGD +EF +HI+TP EKI +++QRI SYIEGKK+HW   PMIV KD
Sbjct: 784 LWTKSIGNYYRSPDMGDGIEFSIHITTPAEKIILIKQRITSYIEGKKDHWYPAPMIVFKD 843

Query: 889 VEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLP 935
           +E LN +RIA+W THRMNHQDMGE+W RR+ LVEE+ KI +ELD++YRL PLDINVR+LP
Sbjct: 844 MESLNSVRIAVWPTHRMNHQDMGEKWARRSQLVEEIAKICRELDIEYRLYPLDINVRNLP 853

BLAST of Tan0022520 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 930.2 bits (2403), Expect = 1.3e-270
Identity = 520/917 (56.71%), Postives = 672/917 (73.28%), Query Frame = 0

Query: 45  MAPSDASDRREVIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFWNDSDGRRN-NGE 104
           MA  D++DRR+ IV I+  + S    +    S    G IW+ESSYDFW+   G+ +  G+
Sbjct: 1   MAAVDSTDRRDFIVNINGQE-SGAVGATGSSSNAEGGNIWKESSYDFWDGEKGKNDKKGD 60

Query: 105 GSNGDGRSEDFEFRQHRK----DVEDPPSKLIGQFLHKQKASG-EMSLDMDMEMLELPLD 164
             + DG S  F  R  R+    ++ DPPSKLIGQFLHKQ+ASG E+SLD+++ M EL  +
Sbjct: 61  DEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN 120

Query: 165 -KTLPPVAESPMRRSSRELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRR 224
               P  A +  RR    +  S   +    + D++RRR      +    GG   ++G  R
Sbjct: 121 TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQ-----NRTSLGGSSDEEGRNR 180

Query: 225 AYGNGHDDSGDEVVRCTSNSSFQRSVSFQRKSSLLRAKTKSRLLDPP-------EQPDRR 284
                 D++  EV++C S           +K  L R KTKSRL DPP       ++ + +
Sbjct: 181 ------DEA--EVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHPAIDKTEMK 240

Query: 285 SGRVPKSGQMRSGLLSKA-----------LDEEDDDPFLEEDLPDEYKKANLGVLTLLQW 344
           SGR  +SG  +SG L K+            +EE++DPFL+EDLP+E+K+  L     L+W
Sbjct: 241 SGR--RSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFLEW 300

Query: 345 ASLILIIAALVCTLTIPYWRQKNLWKLKLWKWEVMILVLICGRLVSGWGIRIIVFFIERN 404
            SL+LI+ +LVC+LTI   ++K  WKL LWKWEV +LVLICGRLVS W +RIIVF +E+N
Sbjct: 301 ISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKN 360

Query: 405 FLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLV 464
           F  RKRVLYFVYGVRK+VQNCLWLGLVL+AW+FLFD KV+RE +S AL YVT+VLVCLLV
Sbjct: 361 FTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALRYVTRVLVCLLV 420

Query: 465 TTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLA 524
             ++WLVKT++VKVLASSFH+STYFDRIQ++LF QYVIETLSGPPL+EIQ+ EEEE+++A
Sbjct: 421 ALIIWLVKTILVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVA 480

Query: 525 EEVIKLQN-AGATIPPDLKATAFSTPKGGRLIGSGGLQRSPRGRSAKLSRVLSKKGD--E 584
           E+V  L+  AGA +PP LKAT  S  K               G+S  L+R+ SK+G+  E
Sbjct: 481 EDVKSLEKLAGAKLPPALKATVKSFMK--------------VGKSPGLNRIGSKRGEDGE 540

Query: 585 GITIDHLHKLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSTHEEDESATQIRSEFEAK 644
           GI ID L +++ KNVSAWNMKRLMNI+  GA+STLD+ ++D+T +EDE AT IRSE+EAK
Sbjct: 541 GIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTT-QEDEDATHIRSEYEAK 600

Query: 645 VAAKKIFQNVARHGSKYIYLEDLMRFMQEDEASKTMSLFEGASESRKISKSSLKNWVVNA 704
            AA+KIF NV   GS+YIYLED +RF+ E+EA + M+LFEGASES KISKS LKNWVV A
Sbjct: 601 CAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWVVKA 660

Query: 705 FRERRALALTLNDTKTAVNKLHRMVNILVSVVILVIWLLILGIATSKFLLFVTSQLVLVA 764
           FRERRALALTLNDTKTAV++LHR++N+++ ++I++IWLLILGIAT++FLL ++SQL+LVA
Sbjct: 661 FRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVA 720

Query: 765 FVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPN 824
           FVFGN+CKT+FEAIIFLFVMHPFDVGDRCEIDGVQ++VEEMNILTT+FLRYDNQKII+PN
Sbjct: 721 FVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPN 780

Query: 825 SVLATKAIHNYYRSPDMGDSVEFCLHISTPPEKIAVMRQRIVSYIEGKKEHWCSTPMIVL 884
           SVL TK I NYYRSPDMGD+VEFC+HI+TPPEKI  ++QRI+SY++ KK++W   PMIV 
Sbjct: 781 SVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVF 840

Query: 885 KDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRS 930
             +++LN ++IA+WLTHRMNHQDMGER+ RR LL+EE+ K  +ELD++YRL PL+INVRS
Sbjct: 841 LSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVRS 875

BLAST of Tan0022520 vs. TAIR 10
Match: AT1G53470.1 (mechanosensitive channel of small conductance-like 4 )

HSP 1 Score: 927.5 bits (2396), Expect = 8.2e-270
Identity = 522/915 (57.05%), Postives = 662/915 (72.35%), Query Frame = 0

Query: 45  MAPSDASDRREVIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFW-NDSDGRRNNGE 104
           MA      RR+ +V+ID  D   N  S          + WRESS +FW ND   +   GE
Sbjct: 1   MAVDSTDQRRDFVVRIDGED---NGDSE---------KFWRESSINFWHNDKSSKPPGGE 60

Query: 105 GSNGDGRSEDFEFRQHRKDVE-DPPSKLIGQFLHKQKASG-EMSLDMDMEMLELPLDKTL 164
             +G   S DF  R   K  E DPPSKLI QFL+KQKASG E+SLDM+  M EL    T+
Sbjct: 61  EDDG---SFDFMRRSSEKSEEPDPPSKLINQFLNKQKASGDEISLDMEANMPELQ-KNTV 120

Query: 165 PPVAESPMRRSSRELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRRAYGN 224
           PP++ + +  S+  +     +       D+IRRR          + G  S+  + R    
Sbjct: 121 PPLSSTAVSGSASPVTAPVTASYRNGTGDAIRRRQNRVTLSPSVKDGDSSEDEENRV--- 180

Query: 225 GHDDSGDEVVRCTSNSSFQRSVSFQRKSSLLRAKTKSRLLDP--PEQPDRRSGRVPKSGQ 284
                G EVV+CTSN S  R+       +L++ KT+SRL+DP  P  PD  SGR P+SG 
Sbjct: 181 ----DGSEVVKCTSNRSTMRT------KTLMKMKTRSRLMDPPTPTYPDMVSGRTPRSGN 240

Query: 285 MRSGLL-------------SKALDEEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILII 344
           +  G               SK L EE++DPF EEDLP+  +K  + V  +++W  LILII
Sbjct: 241 LNPGFSGRNTKPGTPNQGGSKDL-EEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILII 300

Query: 345 AALVCTLTIPYWRQKNLWKLKLWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRV 404
           A+L+C+L IPY R K LW L LWKWEVM+LVLICGRLVS W +++ V+F+E NFL RK+V
Sbjct: 301 ASLICSLVIPYLRGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKV 360

Query: 405 LYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVTTLVWLV 464
           LYFVYG+RK VQNCLWLGLVLIAW+FLFD KV+RE++S  L+YVTKVL+CLLV  ++WL+
Sbjct: 361 LYFVYGIRKPVQNCLWLGLVLIAWHFLFDKKVEREMRSTVLKYVTKVLICLLVAVIIWLI 420

Query: 465 KTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQ 524
           KTL+VKVLASSFH+STYFDRIQ++LF QYVIETLSGPP IEI     EEE++A +V   +
Sbjct: 421 KTLLVKVLASSFHMSTYFDRIQESLFTQYVIETLSGPPRIEI---HIEEEKVANDVKTFE 480

Query: 525 NAGATIPPDLKATAFSTPKGGRLIGSGGLQRSPR--GRSAKLSRVLSKK--GDEGITIDH 584
             G  + P L   A S+P     +GSG LQ+SP   G+S  LSR  SKK  G+EGI IDH
Sbjct: 481 IVGRKLSP-LGPKAVSSPP-QVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDH 540

Query: 585 LHKLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSTHEEDESATQIRSEFEAKVAAKKI 644
           L +++ KNVSAW MK+LMN+++ G LSTLDEQI+D+T +ED+ ATQIRSEFEAK+AA+KI
Sbjct: 541 LQRMNTKNVSAWKMKKLMNVIKKGTLSTLDEQIQDTTTQEDDKATQIRSEFEAKLAARKI 600

Query: 645 FQNVARHGSKYIYLEDLMRFMQEDEASKTMSLFEGASESRKISKSSLKNWVVNAFRERRA 704
           FQNVA  GS+YIY+ED MRF+ EDE+ + M LFEGASE  KISKS LKNWVVNAFRERRA
Sbjct: 601 FQNVAEPGSRYIYMEDFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRA 660

Query: 705 LALTLNDTKTAVNKLHRMVNILVSVVILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNT 764
           LALTLNDTKTAVN+LHR+V++LVS+VIL+IWLLILGIAT+KFLL ++SQL+LV FVFGN+
Sbjct: 661 LALTLNDTKTAVNRLHRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNS 720

Query: 765 CKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATK 824
           CKT+FEA+IF+FVMHPFDVGDRCEIDGVQMIVEEMNILTT+FLR+DNQKI++PNS+L TK
Sbjct: 721 CKTIFEAVIFVFVMHPFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTK 780

Query: 825 AIHNYYRSPDMGDSVEFCLHISTPPEKIAVMRQRIVSYIEGKKEHWCSTPMIVLKDVEEL 884
            I NYYRSPDM D++EF +HI+TPPEK   +RQRI+SY++ KK+HW  +PMIV +D+  L
Sbjct: 781 PIANYYRSPDMQDAIEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGL 840

Query: 885 NRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLP---P 935
           N ++IA+W TH+MNHQ+MGER+ RR  L+EE+ ++ +ELD++YRL PL+INV+SLP   P
Sbjct: 841 NSVKIAMWPTHKMNHQNMGERYVRRGQLLEEIGRLCRELDIEYRLYPLNINVKSLPAATP 880

BLAST of Tan0022520 vs. TAIR 10
Match: AT3G14810.2 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 860.9 bits (2223), Expect = 9.4e-250
Identity = 494/917 (53.87%), Postives = 640/917 (69.79%), Query Frame = 0

Query: 45  MAPSDASDRREVIVKIDQSDASSNASSRAVESVNSNGRIWRESSYDFWNDSDGRRN-NGE 104
           MA  D++DRR+ IV I+  + S    +    S    G IW+ESSYDFW+   G+ +  G+
Sbjct: 1   MAAVDSTDRRDFIVNINGQE-SGAVGATGSSSNAEGGNIWKESSYDFWDGEKGKNDKKGD 60

Query: 105 GSNGDGRSEDFEFRQHRK----DVEDPPSKLIGQFLHKQKASG-EMSLDMDMEMLELPLD 164
             + DG S  F  R  R+    ++ DPPSKLIGQFLHKQ+ASG E+SLD+++ M EL  +
Sbjct: 61  DEDEDGGSFHFRQRGERRHSSAELSDPPSKLIGQFLHKQRASGDEISLDVELNMAELQSN 120

Query: 165 -KTLPPVAESPMRRSSRELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRR 224
               P  A +  RR    +  S   +    + D++RRR      +    GG   ++G  R
Sbjct: 121 TPPRPATASNTPRRGLTTISESSSPVKTKVKADAVRRRQ-----NRTSLGGSSDEEGRNR 180

Query: 225 AYGNGHDDSGDEVVRCTSNSSFQRSVSFQRKSSLLRAKTKSRLLDPP-------EQPDRR 284
                 D++  EV++C S           +K  L R KTKSRL DPP       ++ + +
Sbjct: 181 ------DEA--EVLKCGS-----------KKPMLSRNKTKSRLQDPPTPTHPAIDKTEMK 240

Query: 285 SGRVPKSGQMRSGLLSKA-----------LDEEDDDPFLEEDLPDEYKKANLGVLTLLQW 344
           SGR  +SG  +SG L K+            +EE++DPFL+EDLP+E+K+  L     L+W
Sbjct: 241 SGR--RSGIFKSGFLGKSPKAGTPGRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFLEW 300

Query: 345 ASLILIIAALVCTLTIPYWRQKNLWKLKLWKWEVMILVLICGRLVSGWGIRIIVFFIERN 404
            SL+LI+ +LVC+LTI   ++K  WKL LWKWEV +LVLICGRLVS W +RIIVF +E+N
Sbjct: 301 ISLVLIVTSLVCSLTIHNLQRKTWWKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKN 360

Query: 405 FLLRKRVLYFVYGVRKAVQNCLWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLV 464
           F  RKRVLYFVYGVRK+VQNCLWLGLVL+AW+FLFD KV+RE +S AL            
Sbjct: 361 FTWRKRVLYFVYGVRKSVQNCLWLGLVLLAWHFLFDKKVERETRSTALR----------- 420

Query: 465 TTLVWLVKTLMVKVLASSFHVSTYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLA 524
                                 TYFDRIQ++LF QYVIETLSGPPL+EIQ+ EEEE+++A
Sbjct: 421 ----------------------TYFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVA 480

Query: 525 EEVIKLQN-AGATIPPDLKATAFSTPKGGRLIGSGGLQRSPRGRSAKLSRVLSKKGD--E 584
           E+V  L+  AGA +PP LKAT  S  K               G+S  L+R+ SK+G+  E
Sbjct: 481 EDVKSLEKLAGAKLPPALKATVKSFMK--------------VGKSPGLNRIGSKRGEDGE 540

Query: 585 GITIDHLHKLSPKNVSAWNMKRLMNIVRHGALSTLDEQIKDSTHEEDESATQIRSEFEAK 644
           GI ID L +++ KNVSAWNMKRLMNI+  GA+STLD+ ++D+T +EDE AT IRSE+EAK
Sbjct: 541 GIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQNMQDTT-QEDEDATHIRSEYEAK 600

Query: 645 VAAKKIFQNVARHGSKYIYLEDLMRFMQEDEASKTMSLFEGASESRKISKSSLKNWVVNA 704
            AA+KIF NV   GS+YIYLED +RF+ E+EA + M+LFEGASES KISKS LKNWV  A
Sbjct: 601 CAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAMALFEGASESDKISKSCLKNWV--A 660

Query: 705 FRERRALALTLNDTKTAVNKLHRMVNILVSVVILVIWLLILGIATSKFLLFVTSQLVLVA 764
           FRERRALALTLNDTKTAV++LHR++N+++ ++I++IWLLILGIAT++FLL ++SQL+LVA
Sbjct: 661 FRERRALALTLNDTKTAVDRLHRIINVVIGIIIIIIWLLILGIATTRFLLVLSSQLLLVA 720

Query: 765 FVFGNTCKTVFEAIIFLFVMHPFDVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPN 824
           FVFGN+CKT+FEAIIFLFVMHPFDVGDRCEIDGVQ++VEEMNILTT+FLRYDNQKII+PN
Sbjct: 721 FVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQLVVEEMNILTTVFLRYDNQKIIYPN 780

Query: 825 SVLATKAIHNYYRSPDMGDSVEFCLHISTPPEKIAVMRQRIVSYIEGKKEHWCSTPMIVL 884
           SVL TK I NYYRSPDMGD+VEFC+HI+TPPEKI  ++QRI+SY++ KK++W   PMIV 
Sbjct: 781 SVLGTKPIANYYRSPDMGDAVEFCVHIATPPEKITAIKQRILSYVDNKKDYWYPAPMIVF 840

Query: 885 KDVEELNRMRIAIWLTHRMNHQDMGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRS 930
             +++LN ++IA+WLTHRMNHQDMGER+ RR LL+EE+ K  +ELD++YRL PL+INVRS
Sbjct: 841 LSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLLEEVGKTCRELDIEYRLYPLNINVRS 840

BLAST of Tan0022520 vs. TAIR 10
Match: AT2G17000.1 (Mechanosensitive ion channel family protein )

HSP 1 Score: 817.4 bits (2110), Expect = 1.2e-236
Identity = 472/891 (52.97%), Postives = 625/891 (70.15%), Query Frame = 0

Query: 51  SDRREVIVKIDQSDAS------SNASSRAVESVNSNGRIWRESSYDFWNDSDGRRNNGEG 110
           S  +++I   DQ++ +      +N     +    S+G  +  +SY FW D          
Sbjct: 11  SSYKQIISTGDQNEKTKKKKKLANLDDGDIAKTQSSGSSFDGNSYKFWQDI--------A 70

Query: 111 SNGDGRSEDFEFRQHRKDVEDPPSKLIGQFLHKQKASGEMSLDMDMEMLELPLDKTLPPV 170
           ++   +S  F+F Q+R+                     E++LD++ E  E         V
Sbjct: 71  TDDYTKSGSFDFPQYRE---------------------EITLDVNEETEE------TEDV 130

Query: 171 AESPMRRSSRELKVSFESISEISENDSIRRRHRDSPADEEHRGGQQSQKGDRRAYGNGHD 230
           + +     S+E +V F+  S  + N S   R   S           S    R       +
Sbjct: 131 SNNNNLSGSKETRVFFKINSSGTNNMSGSVRSCTSST-------SFSSATMRLNLEQQLE 190

Query: 231 DSGDEVVRCTSNSSFQRSVSFQRKSSLL-RAKTKSRLLDPPEQPDRR-SGRVPKSGQMRS 290
           D G+ VVRC+S           RK+ L+ RAK +SRL+DPP++ +++ S  +  S Q+RS
Sbjct: 191 DEGEVVVRCSS----------VRKTELVSRAKARSRLIDPPQEEEQQYSSWIGTSDQLRS 250

Query: 291 GLLSKALD--EEDDDPFLEEDLPDEYKKANLGVLTLLQWASLILIIAALVCTLTIPYWRQ 350
           GLL +  D  EE+DD   EED+P EY+K  +  +TLLQW SLI ++ ALV +L +  WR 
Sbjct: 251 GLLGRHSDDIEEEDDSSAEEDVPVEYRKLKMDAITLLQWMSLIALVVALVLSLGLHTWRN 310

Query: 351 KNLWKLKLWKWEVMILVLICGRLVSGWGIRIIVFFIERNFLLRKRVLYFVYGVRKAVQNC 410
             LW L LWKWEV++LVLICGRLVSG GIRIIVFFIERNFLLRKRVLYFVYGV+ AVQNC
Sbjct: 311 ATLWSLHLWKWEVVLLVLICGRLVSGCGIRIIVFFIERNFLLRKRVLYFVYGVKTAVQNC 370

Query: 411 LWLGLVLIAWNFLFDDKVQREVKSNALEYVTKVLVCLLVTTLVWLVKTLMVKVLASSFHV 470
           LWLGLVL+AW+FLFD KV++E +S+ L  ++K+LVC L++T++WL+KTL+VKVLASSFHV
Sbjct: 371 LWLGLVLLAWHFLFDKKVEKETQSDVLLLMSKILVCFLLSTVLWLIKTLVVKVLASSFHV 430

Query: 471 STYFDRIQDALFNQYVIETLSGPPLIEIQKNEEEEERLAEEVIKLQNAGATIPPDLKATA 530
           STYFDRIQ+ALF+ Y+IETLSGPP++E+ + EEEE+R  +E+ K+Q  GA + P+L + A
Sbjct: 431 STYFDRIQEALFHHYLIETLSGPPMLELSRIEEEEDRTQDEIYKMQKGGADLSPELCSAA 490

Query: 531 FSTPKGGRLIGSGGLQRSPRGRSAKLSRVLSKKG-DEGITIDHLHKLSPKNVSAWNMKRL 590
           F   K G  +            + K S ++ K G D GIT+D LHK++ KNVSAWNMKRL
Sbjct: 491 FPQEKSGSTM------------NMKFSPIIPKTGSDNGITMDDLHKMNQKNVSAWNMKRL 550

Query: 591 MNIVRHGALSTLDEQIKDSTHEEDESATQIRSEFEAKVAAKKIFQNVARHGSKYIYLEDL 650
           M IVR+ +LSTLDEQ   +T  EDES  QIRSE EAK AA+KIF+NVA+ G+K+IYLEDL
Sbjct: 551 MKIVRNVSLSTLDEQALQNTC-EDESTRQIRSEKEAKAAARKIFKNVAQPGTKHIYLEDL 610

Query: 651 MRFMQEDEASKTMSLFEGASESRKISKSSLKNWVVNAFRERRALALTLNDTKTAVNKLHR 710
           MRF++ DEA KTM LFEGA  ++KI+KS+LKNW+VNAFRERRALALTLNDTKTAVNKLH 
Sbjct: 611 MRFLRVDEAMKTMCLFEGALVTKKITKSALKNWLVNAFRERRALALTLNDTKTAVNKLHH 670

Query: 711 MVNILVSVVILVIWLLILGIATSKFLLFVTSQLVLVAFVFGNTCKTVFEAIIFLFVMHPF 770
           M++ L ++VI+VIWL++L IATSK+LLF+TSQ+VL+AF+FGN+ KTVFE+IIFLF++HP+
Sbjct: 671 MISFLTAIVIIVIWLILLEIATSKYLLFLTSQVVLLAFMFGNSLKTVFESIIFLFIIHPY 730

Query: 771 DVGDRCEIDGVQMIVEEMNILTTIFLRYDNQKIIFPNSVLATKAIHNYYRSPDMGDSVEF 830
           DVGDR  ID V+M+VEEMNILTT+FLR DN KI++PN +L  KAIHNY RSPDMGD V  
Sbjct: 731 DVGDRLLIDTVEMVVEEMNILTTVFLRADNLKIVYPNILLWQKAIHNYNRSPDMGDEVTC 790

Query: 831 CLHISTPPEKIAVMRQRIVSYIEGKKEHWCSTPMIVLKDVEELNRMRIAIWLTHRMNHQD 890
           C+HI+TPPEKIA ++QRI SYI+ K E+W     +++KDVE+LN +RIAIWL H++NHQ+
Sbjct: 791 CVHITTPPEKIAAIKQRISSYIDSKPEYWYPKADVIVKDVEDLNIVRIAIWLCHKINHQN 836

Query: 891 MGERWTRRALLVEELVKIFQELDLQYRLLPLDINVRSLPPVNSTRLPATWT 931
           MGER+TRRALL+EE++KI  ELD+QYR  PLDINV+++P V S+R+P  W+
Sbjct: 851 MGERFTRRALLIEEVIKILLELDIQYRFHPLDINVKTMPTVVSSRVPPAWS 836