Tan0022503 (gene) Snake gourd v1

Overview
NameTan0022503
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionPectinesterase
LocationLG11: 16026391 .. 16028379 (-)
RNA-Seq ExpressionTan0022503
SyntenyTan0022503
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTCGGGAAGGTGGTCGTTTCGGCAATATCTCTTATTCTTGTCGTCGGTGTAGCTCTCGCCGTCGTCGCCATTGTTAACAGGAGCGATTCCAAAGCTGTGGAGGAGAATCTGTCGCCAAAGATGAAGGCAGTCTCAACGATTTGTGCTGGTACTGATTACCAGAAAGAATGCCAGAATACTCTTGCCAACATTGCTCATAATGCCTCTTCAAATGATCCCAAAGAATATGTTAAAGCGGCAATTCTTGCCACTATTGACGAGGTTACAAAAGGCTTCAACTTGTCTGATACCCTTATGGTGGAAGCTGCTGATAATGCCACCATGAAAATGTCTGTTGATGATTGCAAGGACTTATTGCAGTTTGCCATCGACTCGCTACAGGCATCTTACTCCACCGTTGGGGACCCCGAAATGCACACCAATGCAGATCGCATCGATGATGTCAAGACATGGCTCAGCGCCGTCGTTTCTTTTCAGGAATCTTGCCTCGATGGACTTGGAGAAACCAATCCCCAGCTCAAACAGAAGATGCAAGACGGTCTTGATGTTGCTGGCAAGCTTACCAGCAATGCTCTTGCTATCCTCGATGTAGTTTCTAATGTTCTCCAAAGCTTGGGCTTGCAATTGAAGGTTCTGCTAACTCACTTACTAAACTCAACATGCTTAAGCCCATAAGTTATGGTATATTTGACTGATATGTATTTCTTTTGTTACCAGGTCCAACCAAGTGGCCGTAGGCTTCTTGGAACAACAGAAGTAGGAAGCGACGGGTTCCCGACATGGTTGTCGGGGGCAGACAGGAAGCTTCTGGCAGCAAGAGGAGGTGCCCCTCGAGTGGTACCCAACGCCGTGGTGGCTAAGGACGGTAGTGGACAGTTTAAGACCATTGGTGCTGCATTGGCTGCATACCCAAAGGTCCTAAGGGGAAGATATGTAATCTACGTGAAGGCAGGAGTCTATAATGAATACATCACTGTGACTAAAGACATGGTAAACGTTTACATGTATGGCGATGGCCCAAGAAGAACCATTGTCACTGGCCGCAAGAGCAACCGCGATGGCGTCACCACCCAAGACACTGCCTCTTTCTGTAAGTAACTCCGTCTCAAGGACTTAAAATATTTTCAAATTCTCTTTCAACTCAAAAAGAGTTTTATGTTCCTAAACTGATCTTTCAAATGGGATTTCGAAAATTCTTTGTTTCGCCTGATTTGATTTCTACCTCTAAATCCCTAAATTGTTCGGAAACTAACAATCGCAATTAACACTACAAAATTACCTCGCCTCGCAGCGGCCATTGGAGCGGGATTCCTCTGCAAATCCATGGGATTCCAAAACACAGCCGGCCCCGAAGGCCACCAAGCCGTAGCCCTCAGAAGTCAATCCGATAGATCCGCCTTCTTCAACTGCAGAATGGACGGGTACCAAGACACACTCTACGCTCAAGCCAACCGTCAATTCTACCGCAACTGCGTCATCTCCGGCACCGTGGACTTCATCTTCGGTGACTCAAACACCATCATCCAAAACTCCTTGATCATCGTCCGACGCCCAATGGACAACCAACAGAACACAGTGACAGCTAATGGAAGAAAATACAAGCAAGAGAACTCCGGATTGGTGATCCACAACTGCAGAATCGTGCCAGAGCAGAAGCTGTTCGCGGACAGGTTCAAGATCCCCACATACTTGGGTCGCCCATGGAAGGAATACGCAAGAACCGTGATCATGGAGTCAACCTTGGGTGATTTGGTTCAGCCAGCTGGATACTTGCCATGGGCTGGGAATTTCGCATTGGATACATGCCAGTATTTGGAGTATGCGAACAAAGGACCAGGCGCCAACACGAACAGGAGAGTGAGATGGAAGGGCGTGAGAGTGATTGGAAGAAATGAGGCATTGCAATTCACTGCTGGGCCATTCCTTCTGGGAAAAGCTTGGCTACAGGGCACCGGTGGGCCGTTCTTCATGGGATTGAAGAACTGA

mRNA sequence

ATGGTCGGGAAGGTGGTCGTTTCGGCAATATCTCTTATTCTTGTCGTCGGTGTAGCTCTCGCCGTCGTCGCCATTGTTAACAGGAGCGATTCCAAAGCTGTGGAGGAGAATCTGTCGCCAAAGATGAAGGCAGTCTCAACGATTTGTGCTGGTACTGATTACCAGAAAGAATGCCAGAATACTCTTGCCAACATTGCTCATAATGCCTCTTCAAATGATCCCAAAGAATATGTTAAAGCGGCAATTCTTGCCACTATTGACGAGGTTACAAAAGGCTTCAACTTGTCTGATACCCTTATGGTGGAAGCTGCTGATAATGCCACCATGAAAATGTCTGTTGATGATTGCAAGGACTTATTGCAGTTTGCCATCGACTCGCTACAGGCATCTTACTCCACCGTTGGGGACCCCGAAATGCACACCAATGCAGATCGCATCGATGATGTCAAGACATGGCTCAGCGCCGTCGTTTCTTTTCAGGAATCTTGCCTCGATGGACTTGGAGAAACCAATCCCCAGCTCAAACAGAAGATGCAAGACGGTCTTGATGTTGCTGGCAAGCTTACCAGCAATGCTCTTGCTATCCTCGATGTAGTTTCTAATGTTCTCCAAAGCTTGGGCTTGCAATTGAAGGTCCAACCAAGTGGCCGTAGGCTTCTTGGAACAACAGAAGTAGGAAGCGACGGGTTCCCGACATGGTTGTCGGGGGCAGACAGGAAGCTTCTGGCAGCAAGAGGAGGTGCCCCTCGAGTGGTACCCAACGCCGTGGTGGCTAAGGACGGTAGTGGACAGTTTAAGACCATTGGTGCTGCATTGGCTGCATACCCAAAGGTCCTAAGGGGAAGATATGTAATCTACGTGAAGGCAGGAGTCTATAATGAATACATCACTGTGACTAAAGACATGGTAAACGTTTACATGTATGGCGATGGCCCAAGAAGAACCATTGTCACTGGCCGCAAGAGCAACCGCGATGGCGTCACCACCCAAGACACTGCCTCTTTCTCGGCCATTGGAGCGGGATTCCTCTGCAAATCCATGGGATTCCAAAACACAGCCGGCCCCGAAGGCCACCAAGCCGTAGCCCTCAGAAGTCAATCCGATAGATCCGCCTTCTTCAACTGCAGAATGGACGGGTACCAAGACACACTCTACGCTCAAGCCAACCGTCAATTCTACCGCAACTGCGTCATCTCCGGCACCGTGGACTTCATCTTCGGTGACTCAAACACCATCATCCAAAACTCCTTGATCATCGTCCGACGCCCAATGGACAACCAACAGAACACAGTGACAGCTAATGGAAGAAAATACAAGCAAGAGAACTCCGGATTGGTGATCCACAACTGCAGAATCGTGCCAGAGCAGAAGCTGTTCGCGGACAGGTTCAAGATCCCCACATACTTGGGTCGCCCATGGAAGGAATACGCAAGAACCGTGATCATGGAGTCAACCTTGGGTGATTTGGTTCAGCCAGCTGGATACTTGCCATGGGCTGGGAATTTCGCATTGGATACATGCCAGTATTTGGAGTATGCGAACAAAGGACCAGGCGCCAACACGAACAGGAGAGTGAGATGGAAGGGCGTGAGAGTGATTGGAAGAAATGAGGCATTGCAATTCACTGCTGGGCCATTCCTTCTGGGAAAAGCTTGGCTACAGGGCACCGGTGGGCCGTTCTTCATGGGATTGAAGAACTGA

Coding sequence (CDS)

ATGGTCGGGAAGGTGGTCGTTTCGGCAATATCTCTTATTCTTGTCGTCGGTGTAGCTCTCGCCGTCGTCGCCATTGTTAACAGGAGCGATTCCAAAGCTGTGGAGGAGAATCTGTCGCCAAAGATGAAGGCAGTCTCAACGATTTGTGCTGGTACTGATTACCAGAAAGAATGCCAGAATACTCTTGCCAACATTGCTCATAATGCCTCTTCAAATGATCCCAAAGAATATGTTAAAGCGGCAATTCTTGCCACTATTGACGAGGTTACAAAAGGCTTCAACTTGTCTGATACCCTTATGGTGGAAGCTGCTGATAATGCCACCATGAAAATGTCTGTTGATGATTGCAAGGACTTATTGCAGTTTGCCATCGACTCGCTACAGGCATCTTACTCCACCGTTGGGGACCCCGAAATGCACACCAATGCAGATCGCATCGATGATGTCAAGACATGGCTCAGCGCCGTCGTTTCTTTTCAGGAATCTTGCCTCGATGGACTTGGAGAAACCAATCCCCAGCTCAAACAGAAGATGCAAGACGGTCTTGATGTTGCTGGCAAGCTTACCAGCAATGCTCTTGCTATCCTCGATGTAGTTTCTAATGTTCTCCAAAGCTTGGGCTTGCAATTGAAGGTCCAACCAAGTGGCCGTAGGCTTCTTGGAACAACAGAAGTAGGAAGCGACGGGTTCCCGACATGGTTGTCGGGGGCAGACAGGAAGCTTCTGGCAGCAAGAGGAGGTGCCCCTCGAGTGGTACCCAACGCCGTGGTGGCTAAGGACGGTAGTGGACAGTTTAAGACCATTGGTGCTGCATTGGCTGCATACCCAAAGGTCCTAAGGGGAAGATATGTAATCTACGTGAAGGCAGGAGTCTATAATGAATACATCACTGTGACTAAAGACATGGTAAACGTTTACATGTATGGCGATGGCCCAAGAAGAACCATTGTCACTGGCCGCAAGAGCAACCGCGATGGCGTCACCACCCAAGACACTGCCTCTTTCTCGGCCATTGGAGCGGGATTCCTCTGCAAATCCATGGGATTCCAAAACACAGCCGGCCCCGAAGGCCACCAAGCCGTAGCCCTCAGAAGTCAATCCGATAGATCCGCCTTCTTCAACTGCAGAATGGACGGGTACCAAGACACACTCTACGCTCAAGCCAACCGTCAATTCTACCGCAACTGCGTCATCTCCGGCACCGTGGACTTCATCTTCGGTGACTCAAACACCATCATCCAAAACTCCTTGATCATCGTCCGACGCCCAATGGACAACCAACAGAACACAGTGACAGCTAATGGAAGAAAATACAAGCAAGAGAACTCCGGATTGGTGATCCACAACTGCAGAATCGTGCCAGAGCAGAAGCTGTTCGCGGACAGGTTCAAGATCCCCACATACTTGGGTCGCCCATGGAAGGAATACGCAAGAACCGTGATCATGGAGTCAACCTTGGGTGATTTGGTTCAGCCAGCTGGATACTTGCCATGGGCTGGGAATTTCGCATTGGATACATGCCAGTATTTGGAGTATGCGAACAAAGGACCAGGCGCCAACACGAACAGGAGAGTGAGATGGAAGGGCGTGAGAGTGATTGGAAGAAATGAGGCATTGCAATTCACTGCTGGGCCATTCCTTCTGGGAAAAGCTTGGCTACAGGGCACCGGTGGGCCGTTCTTCATGGGATTGAAGAACTGA

Protein sequence

MVGKVVVSAISLILVVGVALAVVAIVNRSDSKAVEENLSPKMKAVSTICAGTDYQKECQNTLANIAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMVEAADNATMKMSVDDCKDLLQFAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQESCLDGLGETNPQLKQKMQDGLDVAGKLTSNALAILDVVSNVLQSLGLQLKVQPSGRRLLGTTEVGSDGFPTWLSGADRKLLAARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYPKVLRGRYVIYVKAGVYNEYITVTKDMVNVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFSAIGAGFLCKSMGFQNTAGPEGHQAVALRSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQKLFADRFKIPTYLGRPWKEYARTVIMESTLGDLVQPAGYLPWAGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQFTAGPFLLGKAWLQGTGGPFFMGLKN
Homology
BLAST of Tan0022503 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 442.6 bits (1137), Expect = 6.8e-123
Identity = 233/561 (41.53%), Postives = 345/561 (61.50%), Query Frame = 0

Query: 5   VVVSAISLI-LVVGVALAVVAIVNRSDSKAVEENLSPKMKAVSTICAGTDYQKECQNTLA 64
           + +S++ LI +VV V + V    +  DSK   E ++  +KAV  +CA TDY+K C++TL 
Sbjct: 18  ITISSVLLISMVVAVTVGVSLNKHDGDSKGKAE-VNASVKAVKDVCAPTDYRKTCEDTL- 77

Query: 65  NIAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMVEAADNATMKMSVDDCKDLLQFA 124
            I +  ++ DP E VK A   T+ ++T     S T+M    D+ T +M++D CK+L+ +A
Sbjct: 78  -IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRT-RMALDQCKELMDYA 137

Query: 125 IDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQESCLDGLGETNPQLKQKMQDGLD 184
           +D L  S+  +G  E H   + + +++ WLSA +S +E+CL+G   T     + M+  L 
Sbjct: 138 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALK 197

Query: 185 VAGKLTSNALAILDVVSNVLQSLGLQLKVQPSGRRLLGTTEVGSDGFPTWLSGADRKLLA 244
            A +LT N LAI+  +SN +  + +      + RRLL      ++GFP+W+    RKLL 
Sbjct: 198 TAIELTHNGLAIISEMSNFVGQMQIP---GLNSRRLL------AEGFPSWVDQRGRKLLQ 257

Query: 245 ARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYPKVLRGRYVIYVKAGVYNEYITVTKDMV 304
           A      V P+ VVA+DGSGQ+KTI  AL   PK     +V+++KAG+Y EY+ V K M 
Sbjct: 258 AAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMS 317

Query: 305 NVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFSAIGAGFLCKSMGFQNTAGPEGHQAVAL 364
           ++   GDGP +TI++G K+ +DG+TT  TA+ + +G  F+ K++GF+NTAG   HQAVA+
Sbjct: 318 HLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAV 377

Query: 365 RSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRP 424
           R QSD S FFNCR DGYQDTLY  ++RQF+R+C ISGT+DF+FGD+  + QN  ++VR+P
Sbjct: 378 RVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKP 437

Query: 425 MDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQKLFADRFKIPTYLGRPWKEYARTVIME 484
           + NQ   +TA+GRK  +E++G V   C I  E    A +     YLGRPWKEY+RT+IM 
Sbjct: 438 LPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMN 497

Query: 485 STLGDLVQPAGYLPWAGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQFTA 544
           + + D VQP G+ PW G+F L T  Y E  N GPG+    RV W G++ +   + L+FT 
Sbjct: 498 TFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTP 557

Query: 545 GPFLLGKAWLQGTGGPFFMGL 565
             ++ G  W+ G G P+  GL
Sbjct: 558 AQYIQGDDWIPGKGVPYTTGL 565

BLAST of Tan0022503 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 7.5e-122
Identity = 230/563 (40.85%), Postives = 350/563 (62.17%), Query Frame = 0

Query: 4   KVVVSAISLILVVGVALAVV--AIVNRSDSKAVEENLSPKMKAVSTICAGTDYQKECQNT 63
           + V+ +IS +L++ + +AV     VN+SD+ A +E ++  +KA+  +CA TDY++ C++T
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDN-AGDEEITTSVKAIKDVCAPTDYKETCEDT 74

Query: 64  LANIAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMVEAADNATMKMSVDDCKDLLQ 123
           L   A + S  DP E VK A  AT+ +++     S T M+E   +   KM++D CK+L+ 
Sbjct: 75  LRKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMD 134

Query: 124 FAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQESCLDGLGETNPQLKQKMQDG 183
           +AI  L  S+  +G  E H   + +  ++ WLSA +S +++CLDG   T     + ++  
Sbjct: 135 YAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKA 194

Query: 184 LDVAGKLTSNALAILDVVSNVLQSLGLQLKVQPSGRRLLGTTEVGSDGFPTWLSGADRKL 243
           L  A +LT N LA++  +SN L  + +    + + RRLL      S  FP+W+    R+L
Sbjct: 195 LKTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL------SQEFPSWMDARARRL 254

Query: 244 LAARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYPKVLRGRYVIYVKAGVYNEYITVTKD 303
           L A      V P+ VVA+DGSGQ+KTI  AL   PK     +V+++K G+Y EY+ V + 
Sbjct: 255 LNA--PMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRS 314

Query: 304 MVNVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFSAIGAGFLCKSMGFQNTAGPEGHQAV 363
           M ++   GDGP +T+++G KS +DG+TT  TA+ + +G  F+ K++ F+NTAG   HQAV
Sbjct: 315 MTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAV 374

Query: 364 ALRSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVR 423
           A+R  +D S F+NC+ DGYQDTLYA ++RQFYR+C ISGT+DF+FGD+  + QN  ++VR
Sbjct: 375 AIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVR 434

Query: 424 RPMDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQKLFADRFKIPTYLGRPWKEYARTVI 483
           +P+ NQ   +TA+GRK  +E++G V+  C IV E    A + +  TYLGRPWKEY+RT+I
Sbjct: 435 KPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTII 494

Query: 484 MESTLGDLVQPAGYLPWAGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQF 543
           M + + D V P G+ PW G F L+T  Y E  N GPGA   +RV W G++ +   E L+F
Sbjct: 495 MNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKF 554

Query: 544 TAGPFLLGKAWLQGTGGPFFMGL 565
           T   ++ G AW+ G G P+ +GL
Sbjct: 555 TPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Tan0022503 vs. ExPASy Swiss-Prot
Match: Q9SMY6 (Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3702 GN=PME45 PE=2 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 1.7e-121
Identity = 237/529 (44.80%), Postives = 332/529 (62.76%), Query Frame = 0

Query: 43  KAVSTICAGTDYQKECQNTLAN-IAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMV 102
           K + T+C  T Y+  CQNTL N    +    DP+  +K+AI+A  D++ + F    +L  
Sbjct: 93  KIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKT 152

Query: 103 EAADNATMKMSVDDCKDLLQFAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQE 162
           E  D+   K ++  CK L+  A + L  S   + D E++  A  + D+ +WLSAV+S+QE
Sbjct: 153 ENKDD---KDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSWLSAVMSYQE 212

Query: 163 SCLDGLGETNPQLKQKMQDGLDVAGKLTSNALAILDVVSNVLQSL-----GLQLKVQPSG 222
           +C+DG  E   +LK +++   + +  LTSN+LA++  +   L S+      L L+ + S 
Sbjct: 213 TCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKTRLLLEARSSA 272

Query: 223 RRLLGTTEVGSDGFPTWLSGADRKLLAARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYP 282
           +         +D   +WLS  +R++L A      + PNA VAKDGSG F TI AAL A P
Sbjct: 273 KE--------TDHITSWLSNKERRMLKA-VDVKALKPNATVAKDGSGNFTTINAALKAMP 332

Query: 283 KVLRGRYVIYVKAGVYNEYITVTKDMVNVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFS 342
              +GRY IY+K G+Y+E + + K   NV M GDG ++TIVTG KS+   + T  TA+F 
Sbjct: 333 AKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRTFLTATFV 392

Query: 343 AIGAGFLCKSMGFQNTAGPEGHQAVALRSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNC 402
           A G GF+ +SMGF+NTAGPEGHQAVA+R QSDRS F NCR +GYQDTLYA  +RQ+YR+C
Sbjct: 393 AQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTHRQYYRSC 452

Query: 403 VISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQ 462
           VI GTVDFIFGD+  I QN  I +R+ +  Q+NTVTA GR  K + +G VIHNC + P +
Sbjct: 453 VIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHNCTVAPNE 512

Query: 463 KLFADRFKIPTYLGRPWKEYARTVIMESTLGDLVQPAGYLPW-AGNFALDTCQYLEYANK 522
            L   + +  +YLGRPWK ++RTV+MEST+ D++ P G+L W   +FA+DT  Y EY N 
Sbjct: 513 DLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLSYAEYKND 572

Query: 523 GPGANTNRRVRWKGVRVIGRNEALQFTAGPFLLGKAWLQGTGGPFFMGL 565
           GP   T  RV+W G RV+ + EA++FT GPFL G+ W+Q  G P  +GL
Sbjct: 573 GPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of Tan0022503 vs. ExPASy Swiss-Prot
Match: O80722 (Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1)

HSP 1 Score: 431.0 bits (1107), Expect = 2.0e-119
Identity = 252/602 (41.86%), Postives = 353/602 (58.64%), Query Frame = 0

Query: 1   MVGKVVVSAISLILVVGVALAVVAIVNRSDSKAVEENLSPKMKAVSTICAGTDYQKECQN 60
           M+GKVVVS  S++L+VGVA+ VVA +N++     + NLSP+MKAV  IC  T  +  C  
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNG----DANLSPQMKAVQGICQSTSDKASCVK 60

Query: 61  TLANIAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMVEAADNAT--MKMSVDDCKD 120
           TL  +     S DP + +KA +LAT DE+TK  N +    V    + +   K  +D CK 
Sbjct: 61  TLEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKR 120

Query: 121 LLQFAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQESCLDGLGETNPQLKQKM 180
           +  +A++ L      +G+ ++     +ID +K WL  V ++Q  CLD + E +  L++ +
Sbjct: 121 VFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKAI 180

Query: 181 QDGLDVAGKLTSNALAILDVVSNVLQSLGLQLK-----------------------VQPS 240
            +G+  +  LT+NA+ I   V + +  +  ++                          P 
Sbjct: 181 GEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPD 240

Query: 241 G--RRLLGTTEVGSDGFPTWLSGADRKLLA--------ARGGAPRVVPNAVVAKDGSGQF 300
           G  RRLL   ++   G PTW+SGADRKL+A         RGG  RV  N VVAKDGSGQF
Sbjct: 241 GPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQF 300

Query: 301 KTIGAALAAYPKVLRGRYVIYVKAGVYNEYITVTKDMVNVYMYGDGPRRTIVTGRKS--N 360
           KT+  A+ A P+  RGR +IY+KAG+Y E + + K   N++M+GDG R+T+++  +S   
Sbjct: 301 KTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVAL 360

Query: 361 RDGVTTQDTASFSAIGAGFLCKSMGFQNTAGPEGHQAVALRSQSDRSAFFNCRMDGYQDT 420
             G TT  +A+      GF+ K MGF+NTAGP GHQA A+R   DR+  FNCR DGYQDT
Sbjct: 361 SRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQDT 420

Query: 421 LYAQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRKY-KQEN 480
           LY    RQFYRNCV+SGTVDFIFG S T+IQN+LI+VR+    Q NTVTA+G +      
Sbjct: 421 LYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGMK 480

Query: 481 SGLVIHNCRIVPEQKLFADRFKIPTYLGRPWKEYARTVIMESTLGDLVQPAGYLPWAGNF 540
            G+V+ NCRIVP++KL  +R  + TYLGRPWK+++ TVIM + +GDL++P G+  W G  
Sbjct: 481 IGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGES 540

Query: 541 ALDTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQFTAGPFLLGKAWLQGTGGPFFM 565
              +C+Y+EY N+GPGA  NRRV W  V      E   FTA  +L    W+Q    P  +
Sbjct: 541 FHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPVTI 588

BLAST of Tan0022503 vs. ExPASy Swiss-Prot
Match: Q7Y201 (Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3702 GN=PME13 PE=2 SV=2)

HSP 1 Score: 428.7 bits (1101), Expect = 1.0e-118
Identity = 233/524 (44.47%), Postives = 332/524 (63.36%), Query Frame = 0

Query: 43  KAVSTICAGTDYQKECQNTLANIAHNASSND-PKEYVKAAILATIDEVTKGFNLSDTLMV 102
           K + T+C+ T Y + C+ TL N      + D P  ++K+AI A  +++        +L  
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKT 166

Query: 103 EAADNATMKMSVDDCKDLLQFAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQE 162
           E  D+   K +++ CK L++ A +   AS + +   E+++    + D+++WLSAV+S+QE
Sbjct: 167 ENQDD---KDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 163 SCLDGLGETNPQLKQKMQDGLDVAGKLTSNALAILDVVSNVLQSLGLQLKVQPSGRRLLG 222
           +CLDG  E N  LK +++  ++ +  LTSN+LA+   +    ++L   +KV    R LL 
Sbjct: 227 TCLDGFEEGN--LKSEVKTSVNSSQVLTSNSLAL---IKTFTENLSPVMKVVE--RHLL- 286

Query: 223 TTEVGSDGFPTWLSGADRKLLAARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYPKVLRG 282
                 D  P+W+S  DR++L A      + PNA VAKDGSG F TI  AL A P+   G
Sbjct: 287 ------DDIPSWVSNDDRRMLRA-VDVKALKPNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 283 RYVIYVKAGVYNEYITVTKDMVNVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFSAIGAG 342
           RY+IYVK G+Y+EY+TV K   N+ M GDG ++TIVTG KS+   + T  TA+F A G G
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 343 FLCKSMGFQNTAGPEGHQAVALRSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNCVISGT 402
           F+ +SMGF+NTAGPEGHQAVA+R QSDRS F NCR +GYQDTLYA  +RQ+YR+CVI GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 466

Query: 403 VDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQKLFAD 462
           +DFIFGD+  I QN  I +R+ +  Q+NTVTA GR  K + +G V+HNC+I   + L   
Sbjct: 467 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 526

Query: 463 RFKIPTYLGRPWKEYARTVIMESTLGDLVQPAGYLPW-AGNFALDTCQYLEYANKGPGAN 522
           + +  +YLGRPWK Y+RT+IMES + +++ P G+L W   +FA+DT  Y EY NKG   +
Sbjct: 527 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 586

Query: 523 TNRRVRWKGVRVIGRNEALQFTAGPFLLGKAWLQGTGGPFFMGL 565
           T  RV+W G +VI + EAL +T GPFL G  W+  +G P  +GL
Sbjct: 587 TTSRVKWPGFKVINKEEALNYTVGPFLQGD-WISASGSPVKLGL 611

BLAST of Tan0022503 vs. NCBI nr
Match: XP_022990080.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 943.0 bits (2436), Expect = 1.2e-270
Identity = 469/567 (82.72%), Postives = 517/567 (91.18%), Query Frame = 0

Query: 1   MVGKVVVSAISLILVVGVALAVVAIVNRSDSKAVE-ENLSPKMKAVSTICAGTDYQKECQ 60
           MVGKVVVS +S ILVVGVALAVVA+VN+ +  A E + LSPKMKAV++IC+GT+Y KECQ
Sbjct: 1   MVGKVVVSGVSFILVVGVALAVVAVVNKRNDMATEMQTLSPKMKAVASICSGTNYPKECQ 60

Query: 61  NTLANIAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMVEAADNATMKMSVDDCKDL 120
           +TL+  A N++S+DPK+Y+KAAI+ATI+E+TKG NL+DTL VEA DN+T++MSVDDCKDL
Sbjct: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAVDNSTIQMSVDDCKDL 120

Query: 121 LQFAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQESCLDGLGETNPQLKQKMQ 180
           LQ AIDSL AS STVGDP+MHT+ADR+ DVKTWLSAV+SFQ+SCLDGL ET+P L+QKMQ
Sbjct: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180

Query: 181 DGLDVAGKLTSNALAILDVVSNVLQSLGLQLKVQPSGRRLLGTTEVGSDGFPTWLSGADR 240
             LD AGKLTSNALAILD V  +L+ LGLQLKVQPSGRRLLGTTEVGSDGFPTWL G DR
Sbjct: 181 GELDTAGKLTSNALAILDEVVGMLEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLMGEDR 240

Query: 241 KLLAARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYPKVLRGRYVIYVKAGVYNEYITVT 300
           KLLAARGGA RV PNAVVAKDGSGQFKTI AALAAYPKVLRGRYVIYVKAGVYNEYI VT
Sbjct: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGVYNEYIIVT 300

Query: 301 KDMVNVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFSAIGAGFLCKSMGFQNTAGPEGHQ 360
           K+M N++MYGDGPR TIVTGRKSNRDGVTTQD+ASFSAIG GFLCKSMGF NTAGPEGHQ
Sbjct: 301 KNMENIFMYGDGPRLTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQ 360

Query: 361 AVALRSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALR QSDRSAFFNCRMDG+QDTLY QANRQFYRNCVISGTVDFIFGDSNTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQKLFADRFKIPTYLGRPWKEYART 480
           VRRPMDNQ+NTVTANGR   +EN+GLVIHNCRIVPEQKLFADRFKIPT+LGRPWK+YART
Sbjct: 421 VRRPMDNQKNTVTANGRADNRENTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDLVQPAGYLPWAGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDL+QPAGY+PWAGNFALDTC YLEY N+GPGA TNRRV+WKGVRVIGRNEA+
Sbjct: 481 VIMESTLGDLIQPAGYMPWAGNFALDTCLYLEYGNRGPGAITNRRVKWKGVRVIGRNEAM 540

Query: 541 QFTAGPFLLGKAWLQGTGGPFFMGLKN 567
           QFTA PFL G  WLQGTGGPFF+GLKN
Sbjct: 541 QFTAEPFLQGSTWLQGTGGPFFVGLKN 567

BLAST of Tan0022503 vs. NCBI nr
Match: XP_023527159.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 940.6 bits (2430), Expect = 6.1e-270
Identity = 466/567 (82.19%), Postives = 517/567 (91.18%), Query Frame = 0

Query: 1   MVGKVVVSAISLILVVGVALAVVAIVNRSDSKAVE-ENLSPKMKAVSTICAGTDYQKECQ 60
           MVGKVVVS +S ILVVGVALAVVA+VN+ +  A E + LSPKMKAV++IC+GTDY KECQ
Sbjct: 1   MVGKVVVSGVSFILVVGVALAVVAVVNKRNDMATEMQTLSPKMKAVASICSGTDYPKECQ 60

Query: 61  NTLANIAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMVEAADNATMKMSVDDCKDL 120
           +TL+  A N++S+DPK+Y+KAAI+ATI+E+TKG NL+DTL VEAADN+T++MSVDDCKDL
Sbjct: 61  STLSATAQNSTSDDPKQYIKAAIVATIEEITKGLNLTDTLRVEAADNSTIQMSVDDCKDL 120

Query: 121 LQFAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQESCLDGLGETNPQLKQKMQ 180
           LQ AIDSL AS STVGDP+MHT+ADR+ DVKTWLSAV+SFQ+SCLDGL ET+P L+QKMQ
Sbjct: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180

Query: 181 DGLDVAGKLTSNALAILDVVSNVLQSLGLQLKVQPSGRRLLGTTEVGSDGFPTWLSGADR 240
            GLD AGKLTSNALAILD V  +L+ LGLQLKVQPSGRRLLGTTEVGSDGFPTWL G DR
Sbjct: 181 GGLDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLMGEDR 240

Query: 241 KLLAARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYPKVLRGRYVIYVKAGVYNEYITVT 300
           KLLAARGG  RV PNAVVAKDGSGQFKTI AALAAYPKVL GRYVIYVKAGVY+EYI VT
Sbjct: 241 KLLAARGGTGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVT 300

Query: 301 KDMVNVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFSAIGAGFLCKSMGFQNTAGPEGHQ 360
           K+M N++MYGDGPR TIVTGRKSNRDGVTTQD+ASFSAIG GFLCKSMGF NTAGPEGHQ
Sbjct: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQ 360

Query: 361 AVALRSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALR QSD+SAFFNCRMDG+QDTLY QANRQFYRNCVISGTVDFIFGDSNTIIQNSLII
Sbjct: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQKLFADRFKIPTYLGRPWKEYART 480
           VRRPMDNQ+NTVTANGR   ++N+GLVIHNCRIVPEQKLFADRFKIPT+LGRPWK+YART
Sbjct: 421 VRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDLVQPAGYLPWAGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDL+Q AGY+PWAGNFAL+TC YLEY N+GPGA TNRRV+WKGVRVIGRNEA+
Sbjct: 481 VIMESTLGDLIQAAGYMPWAGNFALETCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEAM 540

Query: 541 QFTAGPFLLGKAWLQGTGGPFFMGLKN 567
           QFTAGPFL G  WLQGTGGPFF+GLKN
Sbjct: 541 QFTAGPFLQGNTWLQGTGGPFFVGLKN 567

BLAST of Tan0022503 vs. NCBI nr
Match: XP_022956875.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 940.3 bits (2429), Expect = 7.9e-270
Identity = 467/567 (82.36%), Postives = 516/567 (91.01%), Query Frame = 0

Query: 1   MVGKVVVSAISLILVVGVALAVVAIVN-RSDSKAVEENLSPKMKAVSTICAGTDYQKECQ 60
           M+GKVVVS +S ILVVGVALAVVA+VN R+D+ A  + LSPKMKAV+ IC+GTDY KECQ
Sbjct: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60

Query: 61  NTLANIAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMVEAADNATMKMSVDDCKDL 120
           +TL+  A N++S+DPK+Y+KAAI+ATI+E+TKG NL+DTL VEAADN T++MSVDDCKDL
Sbjct: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120

Query: 121 LQFAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQESCLDGLGETNPQLKQKMQ 180
           LQ AIDSL AS STVGDP+MHT+ADR+ DVKTWLSAV+SFQ+SCLDGL ET+P L+QKMQ
Sbjct: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180

Query: 181 DGLDVAGKLTSNALAILDVVSNVLQSLGLQLKVQPSGRRLLGTTEVGSDGFPTWLSGADR 240
             LD AGKLTSNALAILD V  +L+ LGLQLKVQPSGRRLLGTTEVGSDGFPTWL G DR
Sbjct: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240

Query: 241 KLLAARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYPKVLRGRYVIYVKAGVYNEYITVT 300
           KLLAARGGA RV PNAVVAKDGSGQFKTI AALAAYPKVL GRYVIYVKAGVY+EYI VT
Sbjct: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVT 300

Query: 301 KDMVNVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFSAIGAGFLCKSMGFQNTAGPEGHQ 360
           K+M N++MYGDGPR TIVTGRKSNRDGVTTQD+ASFSAIG GFLCKSMGF NTAGPEGHQ
Sbjct: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQ 360

Query: 361 AVALRSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALR QSD+SAFFNCRMDG+QDTLY QANRQFYRNCVISGTVDFIFGDSNTIIQNSLII
Sbjct: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQKLFADRFKIPTYLGRPWKEYART 480
           VRRPMDNQ+NTVTANGR   ++N+GLVIHNCRIVPEQKLFADRFKIPT+LGRPWK+YART
Sbjct: 421 VRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDLVQPAGYLPWAGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDL+QP GY+PWAGNFALDTC YLEY N+GPGA TNRRV+WKGVRVIGRNEA+
Sbjct: 481 VIMESTLGDLIQPEGYMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEAM 540

Query: 541 QFTAGPFLLGKAWLQGTGGPFFMGLKN 567
           QFTAGPFL G  WLQGTGGPFF+GLKN
Sbjct: 541 QFTAGPFLQGNTWLQGTGGPFFVGLKN 567

BLAST of Tan0022503 vs. NCBI nr
Match: KGN49779.1 (hypothetical protein Csa_017783 [Cucumis sativus])

HSP 1 Score: 934.9 bits (2415), Expect = 3.3e-268
Identity = 460/566 (81.27%), Postives = 512/566 (90.46%), Query Frame = 0

Query: 1   MVGKVVVSAISLILVVGVALAVVAIVNRSDSKAVEENLSPKMKAVSTICAGTDYQKECQN 60
           MVGKVV+S ISLILVVGVALAVVAIVN+SDS     NLSPKMKAV++IC+  DYQ ECQ 
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60

Query: 61  TLANIAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMVEAADNATMKMSVDDCKDLL 120
           TL ++ +N+S  DPKE++KAAI +TI+E+ KG+NL+D LMVEAA+NAT+KMSVDDCKDLL
Sbjct: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120

Query: 121 QFAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQESCLDGLGETNPQLKQKMQD 180
           Q AID L ASYSTVGDP++HTN DRI D+K WL+AV+S+Q+SCLDGL E +PQLKQKMQD
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDVAGKLTSNALAILDVVSNVLQSLGLQLKVQPSGRRLLGTTEVGSDGFPTWLSGADRK 240
           GLDVAGKLTSNALAI+  VS++L+ LGLQLKVQPSGRRLLGTTEV SD FPTWL+G+DRK
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240

Query: 241 LLAARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYPKVLRGRYVIYVKAGVYNEYITVTK 300
           LLAA+ G  RV PN VVAKDGSGQ+KTIGAALAAYPK L+GRYVIYVKAGVYNE I VTK
Sbjct: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300

Query: 301 DMVNVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFSAIGAGFLCKSMGFQNTAGPEGHQA 360
           DM N++MYGDGPR+TIVTGRKSNRDG+TTQ+TASF+AIG GFLCKSMGF NTAGPEGHQA
Sbjct: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420
           VALR QSDRSAFFNCRMDGYQDTLY Q +RQFYRNCVISGTVDFIFGDSNTIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQKLFADRFKIPTYLGRPWKEYARTV 480
           RRPMDNQQNTVTANGR  K+E SGLVIHNCRIVPEQKLFA+RFKIPT+LGRPWKEYARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMESTLGDLVQPAGYLPWAGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQ 540
           IMESTLGD +QPAGY+PW+GNFAL TC Y EY N+GPGANTNRRVRWKGVRVIGRNEA+Q
Sbjct: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGPFLLGKAWLQGTGGPFFMGLKN 567
           FTAGPFLLGKAWL GTGGP+ +GLKN
Sbjct: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of Tan0022503 vs. NCBI nr
Match: XP_038875879.1 (LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida])

HSP 1 Score: 931.8 bits (2407), Expect = 2.8e-267
Identity = 455/566 (80.39%), Postives = 520/566 (91.87%), Query Frame = 0

Query: 1   MVGKVVVSAISLILVVGVALAVVAIVNRSDSKAVEENLSPKMKAVSTICAGTDYQKECQN 60
           MVGKVVVS ISLILVVGVALAVVA+V ++++  V+ + SPKMKAVSTIC+ TDYQ+ECQN
Sbjct: 1   MVGKVVVSGISLILVVGVALAVVAMVTKNNNSDVDLS-SPKMKAVSTICSTTDYQQECQN 60

Query: 61  TLANIAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMVEAADNATMKMSVDDCKDLL 120
           TL N+A NAS +DPKEY KAA+LATI E+TKG+NL+  LMVEAA+NA++KMSVDDCKDLL
Sbjct: 61  TLTNVAQNASVDDPKEYAKAAVLATIQEITKGYNLTADLMVEAANNASIKMSVDDCKDLL 120

Query: 121 QFAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQESCLDGLGETNPQLKQKMQD 180
           Q AID L ASYSTVGDP++HTN DR+ D+K WL+AV+S+Q+SCLDGL E +PQLKQKMQD
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRVADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDVAGKLTSNALAILDVVSNVLQSLGLQLKVQPSGRRLLGTTEVGSDGFPTWLSGADRK 240
           GLDVAGKLTSNALAI+  VS++L++ GLQLKVQPSGRRLL TTE+GSDGFPTW+SGADRK
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKNFGLQLKVQPSGRRLLETTEIGSDGFPTWMSGADRK 240

Query: 241 LLAARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYPKVLRGRYVIYVKAGVYNEYITVTK 300
           LLA+RGG  RV PNAVVAKDGSGQ+K+IGAALAAYPK LRGRYVIYVKAG+Y+EYITVTK
Sbjct: 241 LLASRGGGARVKPNAVVAKDGSGQYKSIGAALAAYPKALRGRYVIYVKAGIYDEYITVTK 300

Query: 301 DMVNVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFSAIGAGFLCKSMGFQNTAGPEGHQA 360
           DM NV+MYGDGPR+TIVTGRK+NRDG+TTQ+TASFSAIG GFLCKSMGF+NTAGPEGHQA
Sbjct: 301 DMKNVFMYGDGPRKTIVTGRKNNRDGITTQNTASFSAIGEGFLCKSMGFRNTAGPEGHQA 360

Query: 361 VALRSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420
           VALR QSDRSAF+NCRMDGYQDTLY Q +RQFYRNCVISGTVDFIFGDS+TIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFYNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQKLFADRFKIPTYLGRPWKEYARTV 480
           RRPMDNQ+NTVTANGR  K+E SGLVIHNCRIVPEQKLFA+RFKIPT+LGRPWK+YARTV
Sbjct: 421 RRPMDNQKNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYARTV 480

Query: 481 IMESTLGDLVQPAGYLPWAGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQ 540
           IME+TLGD +QP GY+PW+GNFAL+TC Y EY N+GPGANTNRRVRWKGVRVIGRNEALQ
Sbjct: 481 IMETTLGDFIQPDGYMPWSGNFALETCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEALQ 540

Query: 541 FTAGPFLLGKAWLQGTGGPFFMGLKN 567
           +TAGPFL+G+AWL  TGGP+ +GLK+
Sbjct: 541 YTAGPFLMGRAWLPATGGPYLLGLKH 565

BLAST of Tan0022503 vs. ExPASy TrEMBL
Match: A0A6J1JP54 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487084 PE=3 SV=1)

HSP 1 Score: 943.0 bits (2436), Expect = 5.9e-271
Identity = 469/567 (82.72%), Postives = 517/567 (91.18%), Query Frame = 0

Query: 1   MVGKVVVSAISLILVVGVALAVVAIVNRSDSKAVE-ENLSPKMKAVSTICAGTDYQKECQ 60
           MVGKVVVS +S ILVVGVALAVVA+VN+ +  A E + LSPKMKAV++IC+GT+Y KECQ
Sbjct: 1   MVGKVVVSGVSFILVVGVALAVVAVVNKRNDMATEMQTLSPKMKAVASICSGTNYPKECQ 60

Query: 61  NTLANIAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMVEAADNATMKMSVDDCKDL 120
           +TL+  A N++S+DPK+Y+KAAI+ATI+E+TKG NL+DTL VEA DN+T++MSVDDCKDL
Sbjct: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAVDNSTIQMSVDDCKDL 120

Query: 121 LQFAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQESCLDGLGETNPQLKQKMQ 180
           LQ AIDSL AS STVGDP+MHT+ADR+ DVKTWLSAV+SFQ+SCLDGL ET+P L+QKMQ
Sbjct: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180

Query: 181 DGLDVAGKLTSNALAILDVVSNVLQSLGLQLKVQPSGRRLLGTTEVGSDGFPTWLSGADR 240
             LD AGKLTSNALAILD V  +L+ LGLQLKVQPSGRRLLGTTEVGSDGFPTWL G DR
Sbjct: 181 GELDTAGKLTSNALAILDEVVGMLEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLMGEDR 240

Query: 241 KLLAARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYPKVLRGRYVIYVKAGVYNEYITVT 300
           KLLAARGGA RV PNAVVAKDGSGQFKTI AALAAYPKVLRGRYVIYVKAGVYNEYI VT
Sbjct: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLRGRYVIYVKAGVYNEYIIVT 300

Query: 301 KDMVNVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFSAIGAGFLCKSMGFQNTAGPEGHQ 360
           K+M N++MYGDGPR TIVTGRKSNRDGVTTQD+ASFSAIG GFLCKSMGF NTAGPEGHQ
Sbjct: 301 KNMENIFMYGDGPRLTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQ 360

Query: 361 AVALRSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALR QSDRSAFFNCRMDG+QDTLY QANRQFYRNCVISGTVDFIFGDSNTIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQKLFADRFKIPTYLGRPWKEYART 480
           VRRPMDNQ+NTVTANGR   +EN+GLVIHNCRIVPEQKLFADRFKIPT+LGRPWK+YART
Sbjct: 421 VRRPMDNQKNTVTANGRADNRENTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDLVQPAGYLPWAGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDL+QPAGY+PWAGNFALDTC YLEY N+GPGA TNRRV+WKGVRVIGRNEA+
Sbjct: 481 VIMESTLGDLIQPAGYMPWAGNFALDTCLYLEYGNRGPGAITNRRVKWKGVRVIGRNEAM 540

Query: 541 QFTAGPFLLGKAWLQGTGGPFFMGLKN 567
           QFTA PFL G  WLQGTGGPFF+GLKN
Sbjct: 541 QFTAEPFLQGSTWLQGTGGPFFVGLKN 567

BLAST of Tan0022503 vs. ExPASy TrEMBL
Match: A0A6J1GYZ3 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111458430 PE=3 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 3.8e-270
Identity = 467/567 (82.36%), Postives = 516/567 (91.01%), Query Frame = 0

Query: 1   MVGKVVVSAISLILVVGVALAVVAIVN-RSDSKAVEENLSPKMKAVSTICAGTDYQKECQ 60
           M+GKVVVS +S ILVVGVALAVVA+VN R+D+ A  + LSPKMKAV+ IC+GTDY KECQ
Sbjct: 1   MIGKVVVSGVSFILVVGVALAVVAVVNKRNDTAAEMQTLSPKMKAVAGICSGTDYPKECQ 60

Query: 61  NTLANIAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMVEAADNATMKMSVDDCKDL 120
           +TL+  A N++S+DPK+Y+KAAI+ATI+E+TKG NL+DTL VEAADN T++MSVDDCKDL
Sbjct: 61  STLSTTAQNSTSDDPKQYLKAAIVATIEEITKGLNLTDTLRVEAADNTTIQMSVDDCKDL 120

Query: 121 LQFAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQESCLDGLGETNPQLKQKMQ 180
           LQ AIDSL AS STVGDP+MHT+ADR+ DVKTWLSAV+SFQ+SCLDGL ET+P L+QKMQ
Sbjct: 121 LQSAIDSLHASQSTVGDPDMHTDADRVGDVKTWLSAVISFQQSCLDGLEETDPDLRQKMQ 180

Query: 181 DGLDVAGKLTSNALAILDVVSNVLQSLGLQLKVQPSGRRLLGTTEVGSDGFPTWLSGADR 240
             LD AGKLTSNALAILD V  +L+ LGLQLKVQPSGRRLLGTTEVGSDGFPTWL G DR
Sbjct: 181 GELDSAGKLTSNALAILDEVVGILEKLGLQLKVQPSGRRLLGTTEVGSDGFPTWLVGEDR 240

Query: 241 KLLAARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYPKVLRGRYVIYVKAGVYNEYITVT 300
           KLLAARGGA RV PNAVVAKDGSGQFKTI AALAAYPKVL GRYVIYVKAGVY+EYI VT
Sbjct: 241 KLLAARGGAGRVKPNAVVAKDGSGQFKTIAAALAAYPKVLTGRYVIYVKAGVYDEYIIVT 300

Query: 301 KDMVNVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFSAIGAGFLCKSMGFQNTAGPEGHQ 360
           K+M N++MYGDGPR TIVTGRKSNRDGVTTQD+ASFSAIG GFLCKSMGF NTAGPEGHQ
Sbjct: 301 KNMENIFMYGDGPRSTIVTGRKSNRDGVTTQDSASFSAIGKGFLCKSMGFSNTAGPEGHQ 360

Query: 361 AVALRSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALR QSD+SAFFNCRMDG+QDTLY QANRQFYRNCVISGTVDFIFGDSNTIIQNSLII
Sbjct: 361 AVALRVQSDKSAFFNCRMDGHQDTLYVQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQKLFADRFKIPTYLGRPWKEYART 480
           VRRPMDNQ+NTVTANGR   ++N+GLVIHNCRIVPEQKLFADRFKIPT+LGRPWK+YART
Sbjct: 421 VRRPMDNQKNTVTANGRADNRQNTGLVIHNCRIVPEQKLFADRFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDLVQPAGYLPWAGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGDL+QP GY+PWAGNFALDTC YLEY N+GPGA TNRRV+WKGVRVIGRNEA+
Sbjct: 481 VIMESTLGDLIQPEGYMPWAGNFALDTCLYLEYGNRGPGAMTNRRVKWKGVRVIGRNEAM 540

Query: 541 QFTAGPFLLGKAWLQGTGGPFFMGLKN 567
           QFTAGPFL G  WLQGTGGPFF+GLKN
Sbjct: 541 QFTAGPFLQGNTWLQGTGGPFFVGLKN 567

BLAST of Tan0022503 vs. ExPASy TrEMBL
Match: A0A0A0KLN2 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.6e-268
Identity = 460/566 (81.27%), Postives = 512/566 (90.46%), Query Frame = 0

Query: 1   MVGKVVVSAISLILVVGVALAVVAIVNRSDSKAVEENLSPKMKAVSTICAGTDYQKECQN 60
           MVGKVV+S ISLILVVGVALAVVAIVN+SDS     NLSPKMKAV++IC+  DYQ ECQ 
Sbjct: 1   MVGKVVMSGISLILVVGVALAVVAIVNKSDSSDETANLSPKMKAVASICSNADYQPECQT 60

Query: 61  TLANIAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMVEAADNATMKMSVDDCKDLL 120
           TL ++ +N+S  DPKE++KAAI +TI+E+ KG+NL+D LMVEAA+NAT+KMSVDDCKDLL
Sbjct: 61  TLGSVGNNSSVEDPKEFIKAAITSTIEEMKKGYNLTDNLMVEAANNATIKMSVDDCKDLL 120

Query: 121 QFAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQESCLDGLGETNPQLKQKMQD 180
           Q AID L ASYSTVGDP++HTN DRI D+K WL+AV+S+Q+SCLDGL E +PQLKQKMQD
Sbjct: 121 QSAIDELHASYSTVGDPDLHTNEDRIADIKNWLTAVISYQQSCLDGLEEFDPQLKQKMQD 180

Query: 181 GLDVAGKLTSNALAILDVVSNVLQSLGLQLKVQPSGRRLLGTTEVGSDGFPTWLSGADRK 240
           GLDVAGKLTSNALAI+  VS++L+ LGLQLKVQPSGRRLLGTTEV SD FPTWL+G+DRK
Sbjct: 181 GLDVAGKLTSNALAIVGAVSDILKELGLQLKVQPSGRRLLGTTEVDSDSFPTWLTGSDRK 240

Query: 241 LLAARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYPKVLRGRYVIYVKAGVYNEYITVTK 300
           LLAA+ G  RV PN VVAKDGSGQ+KTIGAALAAYPK L+GRYVIYVKAGVYNE I VTK
Sbjct: 241 LLAAKRGGVRVKPNVVVAKDGSGQYKTIGAALAAYPKALKGRYVIYVKAGVYNEPIIVTK 300

Query: 301 DMVNVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFSAIGAGFLCKSMGFQNTAGPEGHQA 360
           DM N++MYGDGPR+TIVTGRKSNRDG+TTQ+TASF+AIG GFLCKSMGF NTAGPEGHQA
Sbjct: 301 DMKNIFMYGDGPRKTIVTGRKSNRDGITTQNTASFAAIGEGFLCKSMGFTNTAGPEGHQA 360

Query: 361 VALRSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420
           VALR QSDRSAFFNCRMDGYQDTLY Q +RQFYRNCVISGTVDFIFGDSNTIIQNSLIIV
Sbjct: 361 VALRVQSDRSAFFNCRMDGYQDTLYVQTHRQFYRNCVISGTVDFIFGDSNTIIQNSLIIV 420

Query: 421 RRPMDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQKLFADRFKIPTYLGRPWKEYARTV 480
           RRPMDNQQNTVTANGR  K+E SGLVIHNCRIVPEQKLFA+RFKIPT+LGRPWKEYARTV
Sbjct: 421 RRPMDNQQNTVTANGRADKKEISGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKEYARTV 480

Query: 481 IMESTLGDLVQPAGYLPWAGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQ 540
           IMESTLGD +QPAGY+PW+GNFAL TC Y EY N+GPGANTNRRVRWKGVRVIGRNEA+Q
Sbjct: 481 IMESTLGDFIQPAGYMPWSGNFALATCSYFEYGNRGPGANTNRRVRWKGVRVIGRNEAMQ 540

Query: 541 FTAGPFLLGKAWLQGTGGPFFMGLKN 567
           FTAGPFLLGKAWL GTGGP+ +GLKN
Sbjct: 541 FTAGPFLLGKAWLPGTGGPYLLGLKN 566

BLAST of Tan0022503 vs. ExPASy TrEMBL
Match: A0A5A7U4V7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00110 PE=3 SV=1)

HSP 1 Score: 922.9 bits (2384), Expect = 6.3e-265
Identity = 452/567 (79.72%), Postives = 517/567 (91.18%), Query Frame = 0

Query: 1   MVGKVVVSAISLILVVGVALAVVAIVN-RSDSKAVEENLSPKMKAVSTICAGTDYQKECQ 60
           MVGKV+VSA+SLILVVG+ALAVVA +N R+ S   +E +SPKMKA+S IC+ TDYQ+EC+
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  NTLANIAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMVEAADNATMKMSVDDCKDL 120
           NTL ++AHN SSNDPK+Y KAAILATI E+TKG+NLSD+L+VEA +NA++KMSV+DCKDL
Sbjct: 61  NTLNHVAHNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCKDL 120

Query: 121 LQFAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQESCLDGLGETNPQLKQKMQ 180
           LQFAID LQASYSTVG+ ++HT+ADRI D+K WLSAVVS+Q+SCLDGLGE +PQLKQ+MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVAGKLTSNALAILDVVSNVLQSLGLQLKVQPSGRRLLGTTEVGSDGFPTWLSGADR 240
           DGLDVAGKLTSNALAI+  VSN+L + GLQLKVQPSGRRLLGTTEV SDGFPTWL+GADR
Sbjct: 181 DGLDVAGKLTSNALAIVTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLAARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYPKVLRGRYVIYVKAGVYNEYITVT 300
           KLLA++ G  R  PNAVVAKDGSGQ+KTI AALAAYPK LRGRYVIYVKAG+Y+EYITVT
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMVNVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFSAIGAGFLCKSMGFQNTAGPEGHQ 360
           KDM NV+MYGDGPR+TIVTGRKSNRDG TTQ+TASF+AIG GFLCKSMGF NTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALR QSDRSAFFNCRMDG+QDTLY Q +RQFYRNCVISGTVDFIFGDS+TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQKLFADRFKIPTYLGRPWKEYART 480
           VRRPMDNQQNTVTA GR  ++E +GLVIHNCRIVPEQKLFA+RFKIPT+LGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDLVQPAGYLPWAGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGD +QPAGY+PW+GNFAL+TC YLEY N+GPGANTNRRVRWKGV+VIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 QFTAGPFLLGKAWLQGTGGPFFMGLKN 567
           Q+TAG FLLG+ WL  TGG +++GLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of Tan0022503 vs. ExPASy TrEMBL
Match: A0A1S3CSH2 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503817 PE=3 SV=1)

HSP 1 Score: 919.1 bits (2374), Expect = 9.1e-264
Identity = 451/567 (79.54%), Postives = 515/567 (90.83%), Query Frame = 0

Query: 1   MVGKVVVSAISLILVVGVALAVVAIVN-RSDSKAVEENLSPKMKAVSTICAGTDYQKECQ 60
           MVGKV+VSA+SLILVVG+ALAVVA +N R+ S   +E +SPKMKA+S IC+ TDYQ+EC+
Sbjct: 1   MVGKVIVSALSLILVVGIALAVVATINKRNGSNGGKEEVSPKMKAISAICSTTDYQEECK 60

Query: 61  NTLANIAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMVEAADNATMKMSVDDCKDL 120
           NTL ++A N SSNDPK+Y KAAILATI E+TKG+NLSD+L+VEA +NA++KMSV+DC DL
Sbjct: 61  NTLNHVAQNTSSNDPKDYAKAAILATIKEITKGYNLSDSLIVEAGNNASIKMSVEDCNDL 120

Query: 121 LQFAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQESCLDGLGETNPQLKQKMQ 180
           LQFAID LQASYSTVG+ ++HT+ADRI D+K WLSAVVS+Q+SCLDGLGE +PQLKQ+MQ
Sbjct: 121 LQFAIDELQASYSTVGESDLHTDADRIADIKNWLSAVVSYQQSCLDGLGEFDPQLKQRMQ 180

Query: 181 DGLDVAGKLTSNALAILDVVSNVLQSLGLQLKVQPSGRRLLGTTEVGSDGFPTWLSGADR 240
           DGLDVAGKLTSNALAIL  VSN+L + GLQLKVQPSGRRLLGTTEV SDGFPTWL+GADR
Sbjct: 181 DGLDVAGKLTSNALAILTAVSNILDNYGLQLKVQPSGRRLLGTTEVDSDGFPTWLTGADR 240

Query: 241 KLLAARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYPKVLRGRYVIYVKAGVYNEYITVT 300
           KLLA++ G  R  PNAVVAKDGSGQ+KTI AALAAYPK LRGRYVIYVKAG+Y+EYITVT
Sbjct: 241 KLLASKQGGVRPTPNAVVAKDGSGQYKTIAAALAAYPKALRGRYVIYVKAGIYDEYITVT 300

Query: 301 KDMVNVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFSAIGAGFLCKSMGFQNTAGPEGHQ 360
           KDM NV+MYGDGPR+TIVTGRKSNRDG TTQ+TASF+AIG GFLCKSMGF NTAGP+GHQ
Sbjct: 301 KDMKNVFMYGDGPRKTIVTGRKSNRDGFTTQNTASFAAIGEGFLCKSMGFTNTAGPQGHQ 360

Query: 361 AVALRSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNCVISGTVDFIFGDSNTIIQNSLII 420
           AVALR QSDRSAFFNCRMDG+QDTLY Q +RQFYRNCVISGTVDFIFGDS+TIIQNSLII
Sbjct: 361 AVALRVQSDRSAFFNCRMDGHQDTLYVQTHRQFYRNCVISGTVDFIFGDSSTIIQNSLII 420

Query: 421 VRRPMDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQKLFADRFKIPTYLGRPWKEYART 480
           VRRPMDNQQNTVTA GR  ++E +GLVIHNCRIVPEQKLFA+RFKIPT+LGRPWK+YART
Sbjct: 421 VRRPMDNQQNTVTAQGRAEQKEITGLVIHNCRIVPEQKLFAERFKIPTFLGRPWKQYART 480

Query: 481 VIMESTLGDLVQPAGYLPWAGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEAL 540
           VIMESTLGD +QPAGY+PW+GNFAL+TC YLEY N+GPGANTNRRVRWKGV+VIGRNEAL
Sbjct: 481 VIMESTLGDFIQPAGYMPWSGNFALETCLYLEYGNRGPGANTNRRVRWKGVKVIGRNEAL 540

Query: 541 QFTAGPFLLGKAWLQGTGGPFFMGLKN 567
           Q+TAG FLLG+ WL  TGG +++GLKN
Sbjct: 541 QYTAGAFLLGRTWLPRTGGIYYLGLKN 567

BLAST of Tan0022503 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 442.6 bits (1137), Expect = 4.8e-124
Identity = 233/561 (41.53%), Postives = 345/561 (61.50%), Query Frame = 0

Query: 5   VVVSAISLI-LVVGVALAVVAIVNRSDSKAVEENLSPKMKAVSTICAGTDYQKECQNTLA 64
           + +S++ LI +VV V + V    +  DSK   E ++  +KAV  +CA TDY+K C++TL 
Sbjct: 18  ITISSVLLISMVVAVTVGVSLNKHDGDSKGKAE-VNASVKAVKDVCAPTDYRKTCEDTL- 77

Query: 65  NIAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMVEAADNATMKMSVDDCKDLLQFA 124
            I +  ++ DP E VK A   T+ ++T     S T+M    D+ T +M++D CK+L+ +A
Sbjct: 78  -IKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRT-RMALDQCKELMDYA 137

Query: 125 IDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQESCLDGLGETNPQLKQKMQDGLD 184
           +D L  S+  +G  E H   + + +++ WLSA +S +E+CL+G   T     + M+  L 
Sbjct: 138 LDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGTQGNAGETMKKALK 197

Query: 185 VAGKLTSNALAILDVVSNVLQSLGLQLKVQPSGRRLLGTTEVGSDGFPTWLSGADRKLLA 244
            A +LT N LAI+  +SN +  + +      + RRLL      ++GFP+W+    RKLL 
Sbjct: 198 TAIELTHNGLAIISEMSNFVGQMQIP---GLNSRRLL------AEGFPSWVDQRGRKLLQ 257

Query: 245 ARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYPKVLRGRYVIYVKAGVYNEYITVTKDMV 304
           A      V P+ VVA+DGSGQ+KTI  AL   PK     +V+++KAG+Y EY+ V K M 
Sbjct: 258 AAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQVNKTMS 317

Query: 305 NVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFSAIGAGFLCKSMGFQNTAGPEGHQAVAL 364
           ++   GDGP +TI++G K+ +DG+TT  TA+ + +G  F+ K++GF+NTAG   HQAVA+
Sbjct: 318 HLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAV 377

Query: 365 RSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRP 424
           R QSD S FFNCR DGYQDTLY  ++RQF+R+C ISGT+DF+FGD+  + QN  ++VR+P
Sbjct: 378 RVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKP 437

Query: 425 MDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQKLFADRFKIPTYLGRPWKEYARTVIME 484
           + NQ   +TA+GRK  +E++G V   C I  E    A +     YLGRPWKEY+RT+IM 
Sbjct: 438 LPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSRTIIMN 497

Query: 485 STLGDLVQPAGYLPWAGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQFTA 544
           + + D VQP G+ PW G+F L T  Y E  N GPG+    RV W G++ +   + L+FT 
Sbjct: 498 TFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDILKFTP 557

Query: 545 GPFLLGKAWLQGTGGPFFMGL 565
             ++ G  W+ G G P+  GL
Sbjct: 558 AQYIQGDDWIPGKGVPYTTGL 565

BLAST of Tan0022503 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 439.1 bits (1128), Expect = 5.3e-123
Identity = 230/563 (40.85%), Postives = 350/563 (62.17%), Query Frame = 0

Query: 4   KVVVSAISLILVVGVALAVV--AIVNRSDSKAVEENLSPKMKAVSTICAGTDYQKECQNT 63
           + V+ +IS +L++ + +AV     VN+SD+ A +E ++  +KA+  +CA TDY++ C++T
Sbjct: 15  RYVIISISSVLLISMVVAVTIGVSVNKSDN-AGDEEITTSVKAIKDVCAPTDYKETCEDT 74

Query: 64  LANIAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMVEAADNATMKMSVDDCKDLLQ 123
           L   A + S  DP E VK A  AT+ +++     S T M+E   +   KM++D CK+L+ 
Sbjct: 75  LRKDAKDTS--DPLELVKTAFNATMKQISDVAKKSQT-MIELQKDPRAKMALDQCKELMD 134

Query: 124 FAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQESCLDGLGETNPQLKQKMQDG 183
           +AI  L  S+  +G  E H   + +  ++ WLSA +S +++CLDG   T     + ++  
Sbjct: 135 YAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGNAGETIKKA 194

Query: 184 LDVAGKLTSNALAILDVVSNVLQSLGLQLKVQPSGRRLLGTTEVGSDGFPTWLSGADRKL 243
           L  A +LT N LA++  +SN L  + +    + + RRLL      S  FP+W+    R+L
Sbjct: 195 LKTAVQLTHNGLAMVTEMSNYLGQMQIP---EMNSRRLL------SQEFPSWMDARARRL 254

Query: 244 LAARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYPKVLRGRYVIYVKAGVYNEYITVTKD 303
           L A      V P+ VVA+DGSGQ+KTI  AL   PK     +V+++K G+Y EY+ V + 
Sbjct: 255 LNA--PMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNTTFVVHIKEGIYKEYVQVNRS 314

Query: 304 MVNVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFSAIGAGFLCKSMGFQNTAGPEGHQAV 363
           M ++   GDGP +T+++G KS +DG+TT  TA+ + +G  F+ K++ F+NTAG   HQAV
Sbjct: 315 MTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAV 374

Query: 364 ALRSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVR 423
           A+R  +D S F+NC+ DGYQDTLYA ++RQFYR+C ISGT+DF+FGD+  + QN  ++VR
Sbjct: 375 AIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVR 434

Query: 424 RPMDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQKLFADRFKIPTYLGRPWKEYARTVI 483
           +P+ NQ   +TA+GRK  +E++G V+  C IV E    A + +  TYLGRPWKEY+RT+I
Sbjct: 435 KPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRPWKEYSRTII 494

Query: 484 MESTLGDLVQPAGYLPWAGNFALDTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQF 543
           M + + D V P G+ PW G F L+T  Y E  N GPGA   +RV W G++ +   E L+F
Sbjct: 495 MNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKF 554

Query: 544 TAGPFLLGKAWLQGTGGPFFMGL 565
           T   ++ G AW+ G G P+ +GL
Sbjct: 555 TPAQYIQGDAWIPGKGVPYILGL 562

BLAST of Tan0022503 vs. TAIR 10
Match: AT4G33230.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 438.0 bits (1125), Expect = 1.2e-122
Identity = 237/529 (44.80%), Postives = 332/529 (62.76%), Query Frame = 0

Query: 43  KAVSTICAGTDYQKECQNTLAN-IAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMV 102
           K + T+C  T Y+  CQNTL N    +    DP+  +K+AI+A  D++ + F    +L  
Sbjct: 93  KIIQTLCNSTLYKPTCQNTLKNETKKDTPQTDPRSLLKSAIVAVNDDLDQVFKRVLSLKT 152

Query: 103 EAADNATMKMSVDDCKDLLQFAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQE 162
           E  D+   K ++  CK L+  A + L  S   + D E++  A  + D+ +WLSAV+S+QE
Sbjct: 153 ENKDD---KDAIAQCKLLVDEAKEELGTSMKRINDSEVNNFAKIVPDLDSWLSAVMSYQE 212

Query: 163 SCLDGLGETNPQLKQKMQDGLDVAGKLTSNALAILDVVSNVLQSL-----GLQLKVQPSG 222
           +C+DG  E   +LK +++   + +  LTSN+LA++  +   L S+      L L+ + S 
Sbjct: 213 TCVDGFEE--GKLKTEIRKNFNSSQVLTSNSLAMIKSLDGYLSSVPKVKTRLLLEARSSA 272

Query: 223 RRLLGTTEVGSDGFPTWLSGADRKLLAARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYP 282
           +         +D   +WLS  +R++L A      + PNA VAKDGSG F TI AAL A P
Sbjct: 273 KE--------TDHITSWLSNKERRMLKA-VDVKALKPNATVAKDGSGNFTTINAALKAMP 332

Query: 283 KVLRGRYVIYVKAGVYNEYITVTKDMVNVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFS 342
              +GRY IY+K G+Y+E + + K   NV M GDG ++TIVTG KS+   + T  TA+F 
Sbjct: 333 AKYQGRYTIYIKHGIYDESVIIDKKKPNVTMVGDGSQKTIVTGNKSHAKKIRTFLTATFV 392

Query: 343 AIGAGFLCKSMGFQNTAGPEGHQAVALRSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNC 402
           A G GF+ +SMGF+NTAGPEGHQAVA+R QSDRS F NCR +GYQDTLYA  +RQ+YR+C
Sbjct: 393 AQGEGFMAQSMGFRNTAGPEGHQAVAIRVQSDRSVFLNCRFEGYQDTLYAYTHRQYYRSC 452

Query: 403 VISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQ 462
           VI GTVDFIFGD+  I QN  I +R+ +  Q+NTVTA GR  K + +G VIHNC + P +
Sbjct: 453 VIIGTVDFIFGDAAAIFQNCDIFIRKGLPGQKNTVTAQGRVDKFQTTGFVIHNCTVAPNE 512

Query: 463 KLFADRFKIPTYLGRPWKEYARTVIMESTLGDLVQPAGYLPW-AGNFALDTCQYLEYANK 522
            L   + +  +YLGRPWK ++RTV+MEST+ D++ P G+L W   +FA+DT  Y EY N 
Sbjct: 513 DLKPVKAQFKSYLGRPWKPHSRTVVMESTIEDVIDPVGWLRWQETDFAIDTLSYAEYKND 572

Query: 523 GPGANTNRRVRWKGVRVIGRNEALQFTAGPFLLGKAWLQGTGGPFFMGL 565
           GP   T  RV+W G RV+ + EA++FT GPFL G+ W+Q  G P  +GL
Sbjct: 573 GPSGATAARVKWPGFRVLNKEEAMKFTVGPFLQGE-WIQAIGSPVKLGL 606

BLAST of Tan0022503 vs. TAIR 10
Match: AT2G47030.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 431.0 bits (1107), Expect = 1.5e-120
Identity = 252/602 (41.86%), Postives = 353/602 (58.64%), Query Frame = 0

Query: 1   MVGKVVVSAISLILVVGVALAVVAIVNRSDSKAVEENLSPKMKAVSTICAGTDYQKECQN 60
           M+GKVVVS  S++L+VGVA+ VVA +N++     + NLSP+MKAV  IC  T  +  C  
Sbjct: 1   MIGKVVVSVASILLIVGVAIGVVAFINKNG----DANLSPQMKAVQGICQSTSDKASCVK 60

Query: 61  TLANIAHNASSNDPKEYVKAAILATIDEVTKGFNLSDTLMVEAADNAT--MKMSVDDCKD 120
           TL  +     S DP + +KA +LAT DE+TK  N +    V    + +   K  +D CK 
Sbjct: 61  TLEPV----KSEDPNKLIKAFMLATKDELTKSSNFTGQTEVNMGSSISPNNKAVLDYCKR 120

Query: 121 LLQFAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQESCLDGLGETNPQLKQKM 180
           +  +A++ L      +G+ ++     +ID +K WL  V ++Q  CLD + E +  L++ +
Sbjct: 121 VFMYALEDLATIIEEMGE-DLSQIGSKIDQLKQWLIGVYNYQTDCLDDIEEDD--LRKAI 180

Query: 181 QDGLDVAGKLTSNALAILDVVSNVLQSLGLQLK-----------------------VQPS 240
            +G+  +  LT+NA+ I   V + +  +  ++                          P 
Sbjct: 181 GEGIANSKILTTNAIDIFHTVVSAMAKINNKVDDLKNMTGGIPTPGAPPVVDESPVADPD 240

Query: 241 G--RRLLGTTEVGSDGFPTWLSGADRKLLA--------ARGGAPRVVPNAVVAKDGSGQF 300
           G  RRLL   ++   G PTW+SGADRKL+A         RGG  RV  N VVAKDGSGQF
Sbjct: 241 GPARRLL--EDIDETGIPTWVSGADRKLMAKAGRGRRGGRGGGARVRTNFVVAKDGSGQF 300

Query: 301 KTIGAALAAYPKVLRGRYVIYVKAGVYNEYITVTKDMVNVYMYGDGPRRTIVTGRKS--N 360
           KT+  A+ A P+  RGR +IY+KAG+Y E + + K   N++M+GDG R+T+++  +S   
Sbjct: 301 KTVQQAVDACPENNRGRCIIYIKAGLYREQVIIPKKKNNIFMFGDGARKTVISYNRSVAL 360

Query: 361 RDGVTTQDTASFSAIGAGFLCKSMGFQNTAGPEGHQAVALRSQSDRSAFFNCRMDGYQDT 420
             G TT  +A+      GF+ K MGF+NTAGP GHQA A+R   DR+  FNCR DGYQDT
Sbjct: 361 SRGTTTSLSATVQVESEGFMAKWMGFKNTAGPMGHQAAAIRVNGDRAVIFNCRFDGYQDT 420

Query: 421 LYAQANRQFYRNCVISGTVDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRKY-KQEN 480
           LY    RQFYRNCV+SGTVDFIFG S T+IQN+LI+VR+    Q NTVTA+G +      
Sbjct: 421 LYVNNGRQFYRNCVVSGTVDFIFGKSATVIQNTLIVVRKGSKGQYNTVTADGNELGLGMK 480

Query: 481 SGLVIHNCRIVPEQKLFADRFKIPTYLGRPWKEYARTVIMESTLGDLVQPAGYLPWAGNF 540
            G+V+ NCRIVP++KL  +R  + TYLGRPWK+++ TVIM + +GDL++P G+  W G  
Sbjct: 481 IGIVLQNCRIVPDRKLTPERLTVATYLGRPWKKFSTTVIMSTEMGDLIRPEGWKIWDGES 540

Query: 541 ALDTCQYLEYANKGPGANTNRRVRWKGVRVIGRNEALQFTAGPFLLGKAWLQGTGGPFFM 565
              +C+Y+EY N+GPGA  NRRV W  V      E   FTA  +L    W+Q    P  +
Sbjct: 541 FHKSCRYVEYNNRGPGAFANRRVNWAKV-ARSAAEVNGFTAANWLGPINWIQEANVPVTI 588

BLAST of Tan0022503 vs. TAIR 10
Match: AT2G26450.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 428.7 bits (1101), Expect = 7.2e-120
Identity = 233/524 (44.47%), Postives = 332/524 (63.36%), Query Frame = 0

Query: 43  KAVSTICAGTDYQKECQNTLANIAHNASSND-PKEYVKAAILATIDEVTKGFNLSDTLMV 102
           K + T+C+ T Y + C+ TL N      + D P  ++K+AI A  +++        +L  
Sbjct: 107 KIIQTLCSSTLYMQICEKTLKNRTDKGFALDNPTTFLKSAIEAVNEDLDLVLEKVLSLKT 166

Query: 103 EAADNATMKMSVDDCKDLLQFAIDSLQASYSTVGDPEMHTNADRIDDVKTWLSAVVSFQE 162
           E  D+   K +++ CK L++ A +   AS + +   E+++    + D+++WLSAV+S+QE
Sbjct: 167 ENQDD---KDAIEQCKLLVEDAKEETVASLNKINVTEVNSFEKVVPDLESWLSAVMSYQE 226

Query: 163 SCLDGLGETNPQLKQKMQDGLDVAGKLTSNALAILDVVSNVLQSLGLQLKVQPSGRRLLG 222
           +CLDG  E N  LK +++  ++ +  LTSN+LA+   +    ++L   +KV    R LL 
Sbjct: 227 TCLDGFEEGN--LKSEVKTSVNSSQVLTSNSLAL---IKTFTENLSPVMKVVE--RHLL- 286

Query: 223 TTEVGSDGFPTWLSGADRKLLAARGGAPRVVPNAVVAKDGSGQFKTIGAALAAYPKVLRG 282
                 D  P+W+S  DR++L A      + PNA VAKDGSG F TI  AL A P+   G
Sbjct: 287 ------DDIPSWVSNDDRRMLRA-VDVKALKPNATVAKDGSGDFTTINDALRAMPEKYEG 346

Query: 283 RYVIYVKAGVYNEYITVTKDMVNVYMYGDGPRRTIVTGRKSNRDGVTTQDTASFSAIGAG 342
           RY+IYVK G+Y+EY+TV K   N+ M GDG ++TIVTG KS+   + T  TA+F A G G
Sbjct: 347 RYIIYVKQGIYDEYVTVDKKKANLTMVGDGSQKTIVTGNKSHAKKIRTFLTATFVAQGEG 406

Query: 343 FLCKSMGFQNTAGPEGHQAVALRSQSDRSAFFNCRMDGYQDTLYAQANRQFYRNCVISGT 402
           F+ +SMGF+NTAGPEGHQAVA+R QSDRS F NCR +GYQDTLYA  +RQ+YR+CVI GT
Sbjct: 407 FMAQSMGFRNTAGPEGHQAVAIRVQSDRSIFLNCRFEGYQDTLYAYTHRQYYRSCVIVGT 466

Query: 403 VDFIFGDSNTIIQNSLIIVRRPMDNQQNTVTANGRKYKQENSGLVIHNCRIVPEQKLFAD 462
           +DFIFGD+  I QN  I +R+ +  Q+NTVTA GR  K + +G V+HNC+I   + L   
Sbjct: 467 IDFIFGDAAAIFQNCNIFIRKGLPGQKNTVTAQGRVDKFQTTGFVVHNCKIAANEDLKPV 526

Query: 463 RFKIPTYLGRPWKEYARTVIMESTLGDLVQPAGYLPW-AGNFALDTCQYLEYANKGPGAN 522
           + +  +YLGRPWK Y+RT+IMES + +++ P G+L W   +FA+DT  Y EY NKG   +
Sbjct: 527 KEEYKSYLGRPWKNYSRTIIMESKIENVIDPVGWLRWQETDFAIDTLYYAEYNNKGSSGD 586

Query: 523 TNRRVRWKGVRVIGRNEALQFTAGPFLLGKAWLQGTGGPFFMGL 565
           T  RV+W G +VI + EAL +T GPFL G  W+  +G P  +GL
Sbjct: 587 TTSRVKWPGFKVINKEEALNYTVGPFLQGD-WISASGSPVKLGL 611

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8GX866.8e-12341.53Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3... [more]
Q3E8Z87.5e-12240.85Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Q9SMY61.7e-12144.80Putative pectinesterase/pectinesterase inhibitor 45 OS=Arabidopsis thaliana OX=3... [more]
O807222.0e-11941.86Pectinesterase 4 OS=Arabidopsis thaliana OX=3702 GN=PME4 PE=2 SV=1[more]
Q7Y2011.0e-11844.47Probable pectinesterase/pectinesterase inhibitor 13 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
XP_022990080.11.2e-27082.72pectinesterase-like [Cucurbita maxima][more]
XP_023527159.16.1e-27082.19pectinesterase-like [Cucurbita pepo subsp. pepo][more]
XP_022956875.17.9e-27082.36pectinesterase-like [Cucurbita moschata][more]
KGN49779.13.3e-26881.27hypothetical protein Csa_017783 [Cucumis sativus][more]
XP_038875879.12.8e-26780.39LOW QUALITY PROTEIN: pectinesterase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1JP545.9e-27182.72Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111487084 PE=3 SV=1[more]
A0A6J1GYZ33.8e-27082.36Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111458430 PE=3 SV=1[more]
A0A0A0KLN21.6e-26881.27Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G119700 PE=3 SV=1[more]
A0A5A7U4V76.3e-26579.72Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold180G00110... [more]
A0A1S3CSH29.1e-26479.54Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503817 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G05610.14.8e-12441.53Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G27870.15.3e-12340.85Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT4G33230.11.2e-12244.80Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G47030.11.5e-12041.86Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G26450.17.2e-12044.47Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 40..195
e-value: 1.4E-32
score: 124.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 44..195
e-value: 7.2E-25
score: 88.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 17..198
e-value: 2.9E-19
score: 67.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 42..200
e-value: 3.8E-30
score: 106.8
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 43..199
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 254..549
e-value: 2.7E-128
score: 426.9
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 249..564
e-value: 1.7E-106
score: 358.3
NoneNo IPR availablePANTHERPTHR31707:SF292PECTINESTERASEcoord: 1..564
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 1..564
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 47..201
e-value: 5.0633E-48
score: 162.608
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 398..407
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 254..563

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0022503.1Tan0022503.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity