Tan0022232 (gene) Snake gourd v1

Overview


Name	Tan0022232
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	(R)-mandelonitrile lyase-like
Location	LG06: 659664 .. 661465 (-)
RNA-Seq Expression	Tan0022232
Synteny	Tan0022232

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACTCGTCGCATTTTCTTCTACTCCCAATTCCTCATGTTCTTGGCGATTTTTTACGGTTCATTCTCCATCTCCGCCCATCCAAACCCCCCTCAACATGGTAATACAATTCATCTTGATCAAAACAATTTTCCATTTCCATTAATATAATATTGTGTGTATTGTACAGTAATTAATTTTATCTTGAATGTCCTACAGAGCCCGCCTACCTGAAGTTCGTGTCGAACGCGACGGAATTTCCAGTAGAAGATTACTACGATTACATAATCGTCGGCGGAGGAACAACTGGTTGCCCGTTAGCGGCGACATTATCTAGCAAATTCCGAGTTCTTGTTCTGGAGAGAGGCGGAGTTCCACATGGCAACCCGAATTTAATGTCACAAGAAGGATTCTTGACGACTCTAATGGAAGACGTCCACTCACGTGACTCTCCCGTGCAAGCCTTCACCTCTGAAGAAGGAGTCCCCAACGCACGTGGCCGAATCCTTGGCGGCAGCAGCGCCATCAACGCCGGCTTCTACAGCCGAGCCGACCGAGCATTCTTTCAAAGATCGCACCTGATCAATTGGGACCTCACGATGGTCAACCAGTCCTACGAGTGGGTCGAGAAGGAGATCGTATTCAGACCAAATCTCAAGCACTGGCAATCGGCGGTTCGAGACGGAATGGTCGAAGCCGGAATCAGACCTTACAACGGCTTCAGTTTGGACCATTTGGTGGGGACCAAAATCGGAGGGTCCACATTCGACGAAACGGGCCGCCGCCACAGCGCGGCGGATCTTCTGTCCTATGCGACGCCGTTTAACATCAAAGTGGCCGTGTACGCGACGGTTGAGAGAATTCTCTTGGCTTCAACAAGACCGTTAAGAAGTTCGGCAATTGGGGTTCTTTATCGTGACGAAAAGGGCGCGTATCACCACGCGATGCTACGCGCGCGTGGGGAAGTTTTGTTATGTGCAGGCGCTATAGGAAGTCCACAATTGCTGTTACTGAGTGGAATTGGGTCACGTTCTTATTTGTCGTCGTGGGGGATTCCAGTGAGTCATCATTTACCCTACGTGGGACATTACCTTTACGACAATCCCCGTAACGGGATTTCCATATTGGCGCCAATGCCGTTAGAGCAATCGTTGATACAAGTAGCGGGAATAACGGAATCCGGCGCGTATCTGGAAGCGGCGTCTAATGTAATTCCGTTCACTTCACCATCGAGGTCTAATTTCTTCATAAGAACCCCGTCGGCGCCGGTTTATTTGAATGTGGCGACGTTGATGGAGAAGATAGTCGGGCCGGTGTCGTCCGGTTCGATGAGACTGGCGTCAAGGGATGTGAGAGTGAACCCGGTGGTCCGGTTTAACTACTTCAGGAAGGGGGTGGACTTGGAGAGGTGTGTGAACGGGACGAGGAAGATAGGGGAGATTCTGAGGAGCCGGTCGATGGAGAGATTTAAGTTTAGGGATTGGTTGGGGAGGAGGGAATTCCGGTTCGTGGGGCCGGTTTATCCGGAGGATGATGAGGATGAGAGGCAGTTGGAGTTGGAGTTGAGGAGGTTTTGTAGAGGAAGTGTGAGTACGATATGGCATTACCACGGAGGGTGCGTAATGGGGAGAGTGGTGGAGCGTAATTATGGAGTCATTGGAATGGAAGCGTTGAGAGTGGTCGACGGGTCGACTTTTGGGGTTTCGCCTGGTACTAATCCTCAGGCCACGCTCATGATGCTTGGACGATATGTCGGTCTCCAAATTATCAACGAAAACGAACCACGCTCCACTGCTACTACTTCTACTTTTACTACTCTATAG

mRNA sequence

ATGACTCGTCGCATTTTCTTCTACTCCCAATTCCTCATGTTCTTGGCGATTTTTTACGGTTCATTCTCCATCTCCGCCCATCCAAACCCCCCTCAACATGAGCCCGCCTACCTGAAGTTCGTGTCGAACGCGACGGAATTTCCAGTAGAAGATTACTACGATTACATAATCGTCGGCGGAGGAACAACTGGTTGCCCGTTAGCGGCGACATTATCTAGCAAATTCCGAGTTCTTGTTCTGGAGAGAGGCGGAGTTCCACATGGCAACCCGAATTTAATGTCACAAGAAGGATTCTTGACGACTCTAATGGAAGACGTCCACTCACGTGACTCTCCCGTGCAAGCCTTCACCTCTGAAGAAGGAGTCCCCAACGCACGTGGCCGAATCCTTGGCGGCAGCAGCGCCATCAACGCCGGCTTCTACAGCCGAGCCGACCGAGCATTCTTTCAAAGATCGCACCTGATCAATTGGGACCTCACGATGGTCAACCAGTCCTACGAGTGGGTCGAGAAGGAGATCGTATTCAGACCAAATCTCAAGCACTGGCAATCGGCGGTTCGAGACGGAATGGTCGAAGCCGGAATCAGACCTTACAACGGCTTCAGTTTGGACCATTTGGTGGGGACCAAAATCGGAGGGTCCACATTCGACGAAACGGGCCGCCGCCACAGCGCGGCGGATCTTCTGTCCTATGCGACGCCGTTTAACATCAAAGTGGCCGTGTACGCGACGGTTGAGAGAATTCTCTTGGCTTCAACAAGACCGTTAAGAAGTTCGGCAATTGGGGTTCTTTATCGTGACGAAAAGGGCGCGTATCACCACGCGATGCTACGCGCGCGTGGGGAAGTTTTGTTATGTGCAGGCGCTATAGGAAGTCCACAATTGCTGTTACTGAGTGGAATTGGGTCACGTTCTTATTTGTCGTCGTGGGGGATTCCAGTGAGTCATCATTTACCCTACGTGGGACATTACCTTTACGACAATCCCCGTAACGGGATTTCCATATTGGCGCCAATGCCGTTAGAGCAATCGTTGATACAAGTAGCGGGAATAACGGAATCCGGCGCGTATCTGGAAGCGGCGTCTAATGTAATTCCGTTCACTTCACCATCGAGGTCTAATTTCTTCATAAGAACCCCGTCGGCGCCGGTTTATTTGAATGTGGCGACGTTGATGGAGAAGATAGTCGGGCCGGTGTCGTCCGGTTCGATGAGACTGGCGTCAAGGGATGTGAGAGTGAACCCGGTGGTCCGGTTTAACTACTTCAGGAAGGGGGTGGACTTGGAGAGGTGTGTGAACGGGACGAGGAAGATAGGGGAGATTCTGAGGAGCCGGTCGATGGAGAGATTTAAGTTTAGGGATTGGTTGGGGAGGAGGGAATTCCGGTTCGTGGGGCCGGTTTATCCGGAGGATGATGAGGATGAGAGGCAGTTGGAGTTGGAGTTGAGGAGGTTTTGTAGAGGAAGTGTGAGTACGATATGGCATTACCACGGAGGGTGCGTAATGGGGAGAGTGGTGGAGCGTAATTATGGAGTCATTGGAATGGAAGCGTTGAGAGTGGTCGACGGGTCGACTTTTGGGGTTTCGCCTGGTACTAATCCTCAGGCCACGCTCATGATGCTTGGACGATATGTCGGTCTCCAAATTATCAACGAAAACGAACCACGCTCCACTGCTACTACTTCTACTTTTACTACTCTATAG

Coding sequence (CDS)

ATGACTCGTCGCATTTTCTTCTACTCCCAATTCCTCATGTTCTTGGCGATTTTTTACGGTTCATTCTCCATCTCCGCCCATCCAAACCCCCCTCAACATGAGCCCGCCTACCTGAAGTTCGTGTCGAACGCGACGGAATTTCCAGTAGAAGATTACTACGATTACATAATCGTCGGCGGAGGAACAACTGGTTGCCCGTTAGCGGCGACATTATCTAGCAAATTCCGAGTTCTTGTTCTGGAGAGAGGCGGAGTTCCACATGGCAACCCGAATTTAATGTCACAAGAAGGATTCTTGACGACTCTAATGGAAGACGTCCACTCACGTGACTCTCCCGTGCAAGCCTTCACCTCTGAAGAAGGAGTCCCCAACGCACGTGGCCGAATCCTTGGCGGCAGCAGCGCCATCAACGCCGGCTTCTACAGCCGAGCCGACCGAGCATTCTTTCAAAGATCGCACCTGATCAATTGGGACCTCACGATGGTCAACCAGTCCTACGAGTGGGTCGAGAAGGAGATCGTATTCAGACCAAATCTCAAGCACTGGCAATCGGCGGTTCGAGACGGAATGGTCGAAGCCGGAATCAGACCTTACAACGGCTTCAGTTTGGACCATTTGGTGGGGACCAAAATCGGAGGGTCCACATTCGACGAAACGGGCCGCCGCCACAGCGCGGCGGATCTTCTGTCCTATGCGACGCCGTTTAACATCAAAGTGGCCGTGTACGCGACGGTTGAGAGAATTCTCTTGGCTTCAACAAGACCGTTAAGAAGTTCGGCAATTGGGGTTCTTTATCGTGACGAAAAGGGCGCGTATCACCACGCGATGCTACGCGCGCGTGGGGAAGTTTTGTTATGTGCAGGCGCTATAGGAAGTCCACAATTGCTGTTACTGAGTGGAATTGGGTCACGTTCTTATTTGTCGTCGTGGGGGATTCCAGTGAGTCATCATTTACCCTACGTGGGACATTACCTTTACGACAATCCCCGTAACGGGATTTCCATATTGGCGCCAATGCCGTTAGAGCAATCGTTGATACAAGTAGCGGGAATAACGGAATCCGGCGCGTATCTGGAAGCGGCGTCTAATGTAATTCCGTTCACTTCACCATCGAGGTCTAATTTCTTCATAAGAACCCCGTCGGCGCCGGTTTATTTGAATGTGGCGACGTTGATGGAGAAGATAGTCGGGCCGGTGTCGTCCGGTTCGATGAGACTGGCGTCAAGGGATGTGAGAGTGAACCCGGTGGTCCGGTTTAACTACTTCAGGAAGGGGGTGGACTTGGAGAGGTGTGTGAACGGGACGAGGAAGATAGGGGAGATTCTGAGGAGCCGGTCGATGGAGAGATTTAAGTTTAGGGATTGGTTGGGGAGGAGGGAATTCCGGTTCGTGGGGCCGGTTTATCCGGAGGATGATGAGGATGAGAGGCAGTTGGAGTTGGAGTTGAGGAGGTTTTGTAGAGGAAGTGTGAGTACGATATGGCATTACCACGGAGGGTGCGTAATGGGGAGAGTGGTGGAGCGTAATTATGGAGTCATTGGAATGGAAGCGTTGAGAGTGGTCGACGGGTCGACTTTTGGGGTTTCGCCTGGTACTAATCCTCAGGCCACGCTCATGATGCTTGGACGATATGTCGGTCTCCAAATTATCAACGAAAACGAACCACGCTCCACTGCTACTACTTCTACTTTTACTACTCTATAG

Protein sequence

MTRRIFFYSQFLMFLAIFYGSFSISAHPNPPQHEPAYLKFVSNATEFPVEDYYDYIIVGGGTTGCPLAATLSSKFRVLVLERGGVPHGNPNLMSQEGFLTTLMEDVHSRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADRAFFQRSHLINWDLTMVNQSYEWVEKEIVFRPNLKHWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIGGSTFDETGRRHSAADLLSYATPFNIKVAVYATVERILLASTRPLRSSAIGVLYRDEKGAYHHAMLRARGEVLLCAGAIGSPQLLLLSGIGSRSYLSSWGIPVSHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPSRSNFFIRTPSAPVYLNVATLMEKIVGPVSSGSMRLASRDVRVNPVVRFNYFRKGVDLERCVNGTRKIGEILRSRSMERFKFRDWLGRREFRFVGPVYPEDDEDERQLELELRRFCRGSVSTIWHYHGGCVMGRVVERNYGVIGMEALRVVDGSTFGVSPGTNPQATLMMLGRYVGLQIINENEPRSTATTSTFTTL

Homology

[Close]

BLAST of Tan0022232 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 721.8 bits (1862), Expect = 5.8e-207
Identity = 351/521 (67.37%), Postives = 427/521 (81.96%), Query Frame = 0


Query: 35  PAYLKFVSNATEFPVEDYYDYIIVGGGTTGCPLAATLSSKFRVLVLERGGVPHGNPNLMS 94
           P +++F+SNAT+F  EDYYDYIIVGGGT GCPLAATLS  FRVL+LERGGVP+  PN+MS
Sbjct: 36  PGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMS 95

Query: 95  QEGFLTTLMEDVHSRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADRAFFQRSHL 154
            +GFLTTL  DV++ DSP Q+F SEEGVPNARGR+LGGSSAINAGFYSRAD+ FF+ S L
Sbjct: 96  HDGFLTTL-TDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGL 155

Query: 155 INWDLTMVNQSYEWVEKEIVFRPNLKHWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIGGS 214
           + WDL+ VNQSYEWVE+ IVFRP L+ WQ+A+RD ++E G+ P+NGF+L+H VGTKIGGS
Sbjct: 156 V-WDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGS 215

Query: 215 TFDETGRRHSAADLLSYATPFNIKVAVYATVERILLASTRPLRS---SAIGVLYRDEKGA 274
           TFD TGRRHS+ADLL YA   NI+VAVYATVER+LLAS+  +     SAIGV+YRD+ G 
Sbjct: 216 TFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGR 275

Query: 275 YHHAMLRARGEVLLCAGAIGSPQLLLLSGIGSRSYLSSWGIPVSHHLPYVGHYLYDNPRN 334
           +HHA++R RGEV+L AGA+GSPQLL LSGIG RSYLS+WGIPV+   P+VG ++YDNPRN
Sbjct: 276 FHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRN 335

Query: 335 GISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPSRSNFFIRTPSAPVYLNVATL 394
           GISI+ P+P+E SLIQV G+TE GA+LEAASNVIPF SP  S  FIR P++P+Y+ V T+
Sbjct: 336 GISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHS-VFIRAPASPLYVPVTTI 395

Query: 395 MEKIVGPVSSGSMRLASRDVRVNPVVRFNYFRKGVDLERCVNGTRKIGEILRSRSMERFK 454
           MEKI+GPVS G +RLAS DVR+NPVVRFNYF    DLERCVNGTRKIGEILRSR+M+ F 
Sbjct: 396 MEKILGPVSIGLLRLASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFM 455

Query: 455 FRDWLGRREFRFVGPVYPEDDEDERQLELELRRFCRGSVSTIWHYHGGCVMGRVVERNYG 514
            R+W G R FRFVG   P D  +    +L +  FCR +VSTIWHYHGG V+G+VV+ +  
Sbjct: 456 IREWFGNRRFRFVGAPLPVDQSN----DLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLK 515

Query: 515 VIGMEALRVVDGSTFGVSPGTNPQATLMMLGRYVGLQIINE 553
           VIG+ +LR+VDGSTF +SPGTNPQATLMMLGRY+GL+++ E
Sbjct: 516 VIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRE 549

[Close]

BLAST of Tan0022232 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 5.7e-138
Identity = 255/533 (47.84%), Postives = 352/533 (66.04%), Query Frame = 0


Query: 29  NPPQHEPAYLKFVSNATEFPVEDYYDYIIVGGGTTGCPLAATLSSKFRVLVLERGGVPHG 88
           N   H+ +YL FV +AT+  +E  YDYIIVGGGT GCPLAATLS+ + VLVLERG +P  
Sbjct: 30  NTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGTAGCPLAATLSANYSVLVLERGSLPTE 89

Query: 89  NPNLMSQEGFLTTLMEDVHSRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADRAF 148
            PNL+  +GF+  L ++   + +PV+ F SE+G+ N RGR+LGG+S INAG Y RA+ +F
Sbjct: 90  YPNLLISDGFVYNLQQEDDGK-TPVERFVSEDGIDNVRGRVLGGTSMINAGVYVRANTSF 149

Query: 149 FQRSHLINWDLTMVNQSYEWVEKEIVFRPNLKHWQSAVRDGMVEAGIRPYNGFSLDHLVG 208
           F ++  I WD+ +VNQ+YEWVE  IVF P+ + WQ+ +    +EAGI P NGFS+DHL G
Sbjct: 150 FNQTG-IEWDMDLVNQTYEWVEDTIVFEPDSQTWQTVIGTAYLEAGILPNNGFSVDHLAG 209

Query: 209 TKIGGSTFDETGRRHSAADLLSYATPFNIKVAVYATVERILLASTRPLRSSAIGVLYRDE 268
           T++ GSTFD  G RH++ +LL+   P N++VAV A VE+I+ +S      +AIGV+Y D 
Sbjct: 210 TRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAVEKIIFSSNTS-GVTAIGVIYTDS 269

Query: 269 KGAYHHAMLRARGEVLLCAGAIGSPQLLLLSGIGSRSYLSSWGIPVSHHLPYVGHYLYDN 328
            G  H A +R  GEV+L AG IGSPQLLLLSG+G  SYL+S  I V    PYVG Y+YDN
Sbjct: 270 NGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPESYLTSLNISVVASHPYVGQYIYDN 329

Query: 329 PRNGISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPSRSNFFIRTPSAPV-YLN 388
           PRN I+IL P P+E S + V GIT    + + + + +PF +P  S  F  T S P+    
Sbjct: 330 PRNFINILPPNPIEASTVTVLGITSD--FYQCSISSLPFDTPPFS--FFPTTSYPLPNQT 389

Query: 389 VATLMEKIVGPVSSGSMRL-ASRDVRVNPVVRFNYFRKGVDLERCVNGTRKIGEILRSRS 448
            A ++ K+ GP+S G++ L +S DVRV P V+FNY+    DL  CV+G +K+GE+L + +
Sbjct: 390 FAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSHCVSGMKKLGEVLSTDA 449

Query: 449 MERFKFRDWLGRREFRFVGPVYPEDDEDERQLELELRRFCRGSVSTIWHYHGGCVMGRVV 508
           +E +K  D  G   F  +G   PE+  D+   E     FCR SV++ WHYHGGC++G+V+
Sbjct: 450 LEPYKVEDLPGIDGFNILGIPLPENQTDDAAFE----TFCRESVASYWHYHGGCLVGKVL 509

Query: 509 ERNYGVIGMEALRVVDGSTFGVSPGTNPQATLMMLGRYVGLQIINENEPRSTA 560
           +  + V G+ ALRVVDGSTF  +P ++PQ   +MLGRY+G+QI+ E      A
Sbjct: 510 DDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGIQILQERSASEDA 551

[Close]

BLAST of Tan0022232 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 1.3e-137
Identity = 246/521 (47.22%), Postives = 357/521 (68.52%), Query Frame = 0


Query: 33  HEPAYLKFVSNATEFPVEDYYDYIIVGGGTTGCPLAATLSSKFRVLVLERGGVPHGNPNL 92
           H+ +YL+F  +AT+  +E  YDY+IVGGGT+GCPLAATLS K++VLVLERG +P   PN+
Sbjct: 34  HDFSYLRFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNV 93

Query: 93  MSQEGFLTTLMEDVHSRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADRAFFQRS 152
           ++ +GF+  L ++   + +PV+ F SE+G+ N RGR+LGG+S INAG Y+RA+ + +  S
Sbjct: 94  LTADGFVYNLQQEDDGK-TPVERFVSEDGIDNVRGRVLGGTSMINAGVYARANTSIYSAS 153

Query: 153 HLINWDLTMVNQSYEWVEKEIVFRPNLKHWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIG 212
             ++WD+ +VN++YEWVE  IVF+PN + WQS      +EAG+ P +GFSLDH  GT+I 
Sbjct: 154 G-VDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTGTAFLEAGVDPNHGFSLDHEAGTRIT 213

Query: 213 GSTFDETGRRHSAADLLSYATPFNIKVAVYATVERILLASTRPLRSSAIGVLYRDEKGAY 272
           GSTFD  G RH+A +LL+     N++V V+A+VE+I+ ++   L  +A GV+YRD  G  
Sbjct: 214 GSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGL--TATGVIYRDSNGTP 273

Query: 273 HHAMLRARGEVLLCAGAIGSPQLLLLSGIGSRSYLSSWGIPVSHHLPYVGHYLYDNPRNG 332
           H A +R++GEV++ AG IG+PQLLLLSG+G  SYLSS  IPV    PYVG +L+DNPRN 
Sbjct: 274 HRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNF 333

Query: 333 ISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPSRSNFFIRTPSAPVYLNVATLM 392
           I+IL P P+E +++ V GI  S  + + + + +PFT+P  S FF  T         A   
Sbjct: 334 INILPPNPIEPTIVTVLGI--SNDFYQCSFSSLPFTTPPFS-FFPSTSYPLPNSTFAHFA 393

Query: 393 EKIVGPVSSGSMRL-ASRDVRVNPVVRFNYFRKGVDLERCVNGTRKIGEILRSRSMERFK 452
            K+ GP+S GS+ L +S +VRV+P V+FNY+    DL  CV+G +KIGE+L + +++ +K
Sbjct: 394 SKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCVSGMKKIGELLSTDALKPYK 453

Query: 453 FRDWLGRREFRFVGPVYPEDDEDERQLELELRRFCRGSVSTIWHYHGGCVMGRVVERNYG 512
             D  G   F  +G   P+D  D+   E     FCR SV++ WHYHGGC++G+V++ ++ 
Sbjct: 454 VEDLPGIEGFNILGIPLPKDQTDDAAFE----TFCRESVASYWHYHGGCLVGKVLDGDFR 513

Query: 513 VIGMEALRVVDGSTFGVSPGTNPQATLMMLGRYVGLQIINE 553
           V G++ALRVVDGSTF  +P ++PQ   +MLGRYVG++I+ E
Sbjct: 514 VTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQE 543

[Close]

BLAST of Tan0022232 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 4.9e-137
Identity = 247/524 (47.14%), Postives = 357/524 (68.13%), Query Frame = 0


Query: 33  HEPAYLKFVSNATEFPVEDYYDYIIVGGGTTGCPLAATLSSKFRVLVLERGGVPHGNPNL 92
           H+ +YL F  +AT+  +E  YDY+IVGGGT+GCPLAATLS K++VLVLERG +P   PN+
Sbjct: 34  HDFSYLSFAYDATDLELEGSYDYVIVGGGTSGCPLAATLSEKYKVLVLERGSLPTAYPNV 93

Query: 93  MSQEGFLTTLMEDVHSRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADRAFFQRS 152
           ++ +GF+  L ++   + +PV+ F SE+G+ N RGR+LGG+S INAG Y+RA+ + +  S
Sbjct: 94  LTADGFVYNLQQEDDGK-TPVERFVSEDGIDNVRGRVLGGTSIINAGVYARANTSIYSAS 153

Query: 153 HLINWDLTMVNQSYEWVEKEIVFRPNLKHWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIG 212
             ++WD+ +VNQ+YEWVE  IV++PN + WQS  +   +EAG+ P +GFSLDH  GT+I 
Sbjct: 154 G-VDWDMDLVNQTYEWVEDTIVYKPNSQSWQSVTKTAFLEAGVHPNHGFSLDHEEGTRIT 213

Query: 213 GSTFDETGRRHSAADLLSYATPFNIKVAVYATVERILLASTRPLRSSAIGVLYRDEKGAY 272
           GSTFD  G RH+A +LL+     N++V V+A+VE+I+ ++   L  +A GV+YRD  G  
Sbjct: 214 GSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSNAPGL--TATGVIYRDSNGTP 273

Query: 273 HHAMLRARGEVLLCAGAIGSPQLLLLSGIGSRSYLSSWGIPVSHHLPYVGHYLYDNPRNG 332
           H A +R++GEV++ AG IG+PQLLLLSG+G  SYLSS  IPV    PYVG +L+DNPRN 
Sbjct: 274 HQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPVVLSHPYVGQFLHDNPRNF 333

Query: 333 ISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPSRSNFFIRTPSAPVYL---NVA 392
           I+IL P P+E +++ V GI  S  + + + + +PFT+P    F    PSA   L     A
Sbjct: 334 INILPPNPIEPTIVTVLGI--SNDFYQCSFSSLPFTTPPFGFF----PSASYPLPNSTFA 393

Query: 393 TLMEKIVGPVSSGSMRL-ASRDVRVNPVVRFNYFRKGVDLERCVNGTRKIGEILRSRSME 452
               K+ GP+S GS+ L +S +VRV+P V+FNY+    DL  CV+G +KIGE+L + +++
Sbjct: 394 HFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLTDLSHCVSGMKKIGELLSTDALK 453

Query: 453 RFKFRDWLGRREFRFVGPVYPEDDEDERQLELELRRFCRGSVSTIWHYHGGCVMGRVVER 512
            +K  D  G   F  +G   P+D  D+   E     FCR SV++ WHYHGGC++G+V++ 
Sbjct: 454 PYKVEDLPGVEGFNILGIPLPKDQTDDAAFE----TFCRESVASYWHYHGGCLVGKVLDG 513

Query: 513 NYGVIGMEALRVVDGSTFGVSPGTNPQATLMMLGRYVGLQIINE 553
           ++ V G+ ALRVVDGSTF  +P ++PQ   +MLGRYVG++I+ E
Sbjct: 514 DFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQE 543

[Close]

BLAST of Tan0022232 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 3.1e-136
Identity = 254/552 (46.01%), Postives = 367/552 (66.49%), Query Frame = 0


Query: 11  FLMFLAIFYGSFS-ISAHPNPPQHEPAYLKFVSNATEFPVEDYYDYIIVGGGTTGCPLAA 70
           F++ L + +  +S + +  N   H+ +YLKFV NAT+  +E  YDYI++GGGT+GCPLAA
Sbjct: 11  FVLHLLVLHLQYSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYDYIVIGGGTSGCPLAA 70

Query: 71  TLSSKFRVLVLERGGVPHGNPNLMSQEGFLTTLMEDVHSRDSPVQAFTSEEGVPNARGRI 130
           TLS K++VL+LERG +    PN ++ +GF   L +    + +PV+ F SE+G+ N R RI
Sbjct: 71  TLSEKYKVLLLERGTIATEYPNTLTADGFAYNLQQQDDGK-TPVERFVSEDGIDNVRARI 130

Query: 131 LGGSSAINAGFYSRADRAFFQRSHLINWDLTMVNQSYEWVEKEIVFRPNLKHWQSAVRDG 190
           LGG++ INAG Y+RA+ +F+ ++  I WDL +VN++YEWVE  IV +PN + WQS + +G
Sbjct: 131 LGGTTIINAGVYARANISFYSQTG-IEWDLDLVNKTYEWVEDAIVVKPNNQSWQSVIGEG 190

Query: 191 MVEAGIRPYNGFSLDHLVGTKIGGSTFDETGRRHSAADLLSYATPFNIKVAVYATVERIL 250
            +EAGI P NGFSLDH  GT++ GSTFD  G RH+A +LL+   P N+ VAV A+VE+IL
Sbjct: 191 FLEAGILPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAVQASVEKIL 250

Query: 251 LASTRPLRSSAIGVLYRDEKGAYHHAMLRARGEVLLCAGAIGSPQLLLLSGIGSRSYLSS 310
            +S      SAIGV+Y D  G  H A +R  GEV++ AG IG+PQLLLLSG+G  SYLSS
Sbjct: 251 FSSNTS-NLSAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLSGVGPESYLSS 310

Query: 311 WGIPVSHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTS 370
             I V    PYVG +LY+NPRN I+   P P+E S++ V GI     Y + + + +PF++
Sbjct: 311 LNITVVQPNPYVGQFLYNNPRNFINNFPPNPIEASVVTVLGIRSD--YYQVSLSSLPFST 370

Query: 371 PSRSNFFIRTPSAPV-YLNVATLMEKIVGPVSSGSMRL-ASRDVRVNPVVRFNYFRKGVD 430
           P  S F   T S P+     A ++ ++ GP+S GS+ L +S DVR+ P ++FNY+    D
Sbjct: 371 PPFSLF--PTTSYPLPNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAPNIKFNYYSNSTD 430

Query: 431 LERCVNGTRKIGEILRSRSMERFKFRDWLGRREFRFVGPVYPEDDEDERQLELELRRFCR 490
           L  CV+G +K+G++LR++++E +K RD LG   F ++G   PE+  D+   E     FC 
Sbjct: 431 LANCVSGMKKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDDASFE----TFCL 490

Query: 491 GSVSTIWHYHGGCVMGRVVERNYGVIGMEALRVVDGSTFGVSPGTNPQATLMMLGRYVGL 550
            +V++ WHYHGG ++G+V++ ++ V+G++ALRVVD STF   P ++PQ   +MLGRYVGL
Sbjct: 491 DNVASYWHYHGGSLVGKVLDDSFRVMGIKALRVVDASTFPYEPNSHPQGFYLMLGRYVGL 550

Query: 551 QIINENEPRSTA 560
           QI+ E   R  A
Sbjct: 551 QILQERSIRLEA 551

[Close]

BLAST of Tan0022232 vs. NCBI nr
Match: XP_038899448.1 ((R)-mandelonitrile lyase-like [Benincasa hispida])

HSP 1 Score: 948.7 bits (2451), Expect = 2.2e-272
Identity = 484/562 (86.12%), Postives = 506/562 (90.04%), Query Frame = 0


Query: 1   MTRRIFFYSQFLMFLAIFYGSFSISAHPNPPQHEPAYLKFVSNATEFPVEDYYDYIIVGG 60
           M  R FF+SQFL FL IFY SF   AHPN  Q EP YLKFV+NATEFP EDYYDYIIVGG
Sbjct: 19  MAPRFFFHSQFLAFLLIFYASF---AHPNSSQREPDYLKFVANATEFPEEDYYDYIIVGG 78

Query: 61  GTTGCPLAATLSSKFRVLVLERGGVPHGNPNLMSQEGFLTTLMEDVHSRDSPVQAFTSEE 120
           GT GCPLAATLSSKFRVLVLERGGVPHGN NLMSQEGFLTTLM+DVHSRDSPVQAFTSEE
Sbjct: 79  GTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRDSPVQAFTSEE 138

Query: 121 GVPNARGRILGGSSAINAGFYSRADRAFFQRSHLINWDLTMVNQSYEWVEKEIVFRPNLK 180
           GVPNARGRILGGSSAINAGFYSRAD+AFF+ S L NWDL MVNQSYEWVEKEIVFRPNLK
Sbjct: 139 GVPNARGRILGGSSAINAGFYSRADQAFFKNSRL-NWDLAMVNQSYEWVEKEIVFRPNLK 198

Query: 181 HWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIGGSTFDETGRRHSAADLLSYATPFNIKVA 240
           +WQSAVRDGMVE GI PYNGFSLDHL+GTKIGGSTFDE GRRHSAADLLSYATP NIKVA
Sbjct: 199 NWQSAVRDGMVEVGINPYNGFSLDHLIGTKIGGSTFDELGRRHSAADLLSYATPINIKVA 258

Query: 241 VYATVERILLASTRPL-RSSAIGVLYRDEKGAYHHAMLRARGEVLLCAGAIGSPQLLLLS 300
           VYATVERILLAS  PL R++AIGVLYRD+KGAYHHAMLRARGEVL+ AGAIGSPQLLLLS
Sbjct: 259 VYATVERILLASGGPLRRTTAIGVLYRDQKGAYHHAMLRARGEVLVSAGAIGSPQLLLLS 318

Query: 301 GIGSRSYLSSWGIPVSHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLE 360
           GIGSR YLSSWGIPV+HHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLE
Sbjct: 319 GIGSRPYLSSWGIPVTHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLE 378

Query: 361 AASNVIPFTSPSRSNFFIRTPSAPVYLNVATLMEKIVGPVSSGSMRLASRDVRVNPVVRF 420
           AASNVIPFTSP RS FFIRTPSAPVYL VATLMEKI GPVSSGSMRLAS DVRVNPVVRF
Sbjct: 379 AASNVIPFTSPPRS-FFIRTPSAPVYLTVATLMEKITGPVSSGSMRLASTDVRVNPVVRF 438

Query: 421 NYFRKGVDLERCVNGTRKIGEILRSRSMERFKFRDWLGRREFRFVGPVYPEDDEDERQLE 480
           NYF   VDLERCVNGTRKIGEILRSRSME FKFRDWLGR EFRFVGPVYPE+++++  + 
Sbjct: 439 NYFGNAVDLERCVNGTRKIGEILRSRSMEGFKFRDWLGRSEFRFVGPVYPENEDEDGSM- 498

Query: 481 LELRRFCRGSVSTIWHYHGGCVMGRVVERNYGVIGMEALRVVDGSTFGVSPGTNPQATLM 540
             LR FCR SVSTIWHYHGGCV G+VV+ N  VIGMEALRVVDGS FGVSPGTNPQATLM
Sbjct: 499 --LREFCRRSVSTIWHYHGGCVAGKVVDSNSRVIGMEALRVVDGSIFGVSPGTNPQATLM 558

Query: 541 MLGRYVGLQIINENEPRSTATT 562
           MLGR+VGLQIIN+   RS  TT
Sbjct: 559 MLGRHVGLQIINQ---RSAITT 569

[Close]

BLAST of Tan0022232 vs. NCBI nr
Match: XP_023000184.1 ((R)-mandelonitrile lyase-like [Cucurbita maxima])

HSP 1 Score: 925.6 bits (2391), Expect = 2.0e-265
Identity = 471/562 (83.81%), Postives = 499/562 (88.79%), Query Frame = 0


Query: 1   MTRRIFFYSQFLMFLAIFYGSFSISAHPNPPQHEPAYLKFVSNATEFPVEDYYDYIIVGG 60
           M  RIFFYSQFL+F AIFY S SISA    P   P Y+KFVSNATEFPV+DYYDYIIVGG
Sbjct: 1   MILRIFFYSQFLVFFAIFYASSSISADRRSPWEGPKYMKFVSNATEFPVKDYYDYIIVGG 60

Query: 61  GTTGCPLAATLSSKFRVLVLERGGVPHGNPNLMSQEGFLTTLMEDVHSRDSPVQAFTSEE 120
           GTTGCPLAATLSS +RVLVLERGGVP+GN NLMSQEGFL TLM++ +SRDSPVQAFTSEE
Sbjct: 61  GTTGCPLAATLSSHYRVLVLERGGVPYGNSNLMSQEGFLMTLMDEAYSRDSPVQAFTSEE 120

Query: 121 GVPNARGRILGGSSAINAGFYSRADRAFFQRSHLINWDLTMVNQSYEWVEKEIVFRPNLK 180
           GVPNARGRILGGSSAINAGFYSRADRAFF RSHL NWDLTMVNQSYEWVEK+IVF+PNLK
Sbjct: 121 GVPNARGRILGGSSAINAGFYSRADRAFFHRSHL-NWDLTMVNQSYEWVEKKIVFKPNLK 180

Query: 181 HWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIGGSTFDETGRRHSAADLLSYATPFNIKVA 240
           +WQS VRDGMVEAGI PYNGF+LDHLVGTKIGGSTFDE GRRHSAADLLSYATPFNI+VA
Sbjct: 181 NWQSVVRDGMVEAGINPYNGFTLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIQVA 240

Query: 241 VYATVERILLASTRPLRSSAIGVLYRDEKGAYHHAMLRARGEVLLCAGAIGSPQLLLLSG 300
           VYATVERILL ST     +A+GVLYRD++GAYHHA+L ARGEVLL AGAIGSPQLLLLSG
Sbjct: 241 VYATVERILLTSTGQFGRAAVGVLYRDQRGAYHHAILCARGEVLLSAGAIGSPQLLLLSG 300

Query: 301 IGSRSYLSSWGIPVSHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEA 360
           IGSR YLSSWGIPV+HHLP+VGH LYDNPRNGISILAPMPLEQSLIQVAGITESG+YLEA
Sbjct: 301 IGSRPYLSSWGIPVAHHLPFVGHNLYDNPRNGISILAPMPLEQSLIQVAGITESGSYLEA 360

Query: 361 ASNVIPFTSPSRSNFFIRTPSAPVYLNVATLMEKIVGPVSSGSMRLASRDVRVNPVVRFN 420
           ASNVIPFTSP RS FFI+TPSAPVYL VATLMEKI GPVS GSMRLAS DVR NPVVRFN
Sbjct: 361 ASNVIPFTSPPRS-FFIKTPSAPVYLTVATLMEKIAGPVSFGSMRLASTDVRTNPVVRFN 420

Query: 421 YFRKGVDLERCVNGTRKIGEILRSRSMERFKFRDWLGRREFRFVGPVYPEDDEDERQLEL 480
           YF   VDLERCVNG RKIGE+LRSRSME FKFRD  GRREFRFVGPVYP D       E 
Sbjct: 421 YFSNTVDLERCVNGMRKIGEVLRSRSMESFKFRDRSGRREFRFVGPVYPAD-------EG 480

Query: 481 ELRRFCRGSVSTIWHYHGGCVMGRVVERNYGVIGMEALRVVDGSTFGVSPGTNPQATLMM 540
            LR FCR SVSTIWHYHGGCV G+VVERNY VIGM+ALRVVDGSTFGVSPGTNPQATLMM
Sbjct: 481 RLREFCRESVSTIWHYHGGCVTGKVVERNYKVIGMKALRVVDGSTFGVSPGTNPQATLMM 540

Query: 541 LGRYVGLQIINENEPRSTATTS 563
           LGRYVGLQIIN+   RS+ATT+
Sbjct: 541 LGRYVGLQIINQR--RSSATTT 551

[Close]

BLAST of Tan0022232 vs. NCBI nr
Match: XP_022964434.1 ((R)-mandelonitrile lyase-like [Cucurbita moschata])

HSP 1 Score: 925.2 bits (2390), Expect = 2.6e-265
Identity = 470/562 (83.63%), Postives = 499/562 (88.79%), Query Frame = 0


Query: 1   MTRRIFFYSQFLMFLAIFYGSFSISAHPNPPQHEPAYLKFVSNATEFPVEDYYDYIIVGG 60
           M  RIFFYSQFL+F AIFY S SISA    P   P Y+KFVSNATEFPV+DYYDYIIVGG
Sbjct: 1   MILRIFFYSQFLVFFAIFYASSSISADRRSPWEGPKYMKFVSNATEFPVKDYYDYIIVGG 60

Query: 61  GTTGCPLAATLSSKFRVLVLERGGVPHGNPNLMSQEGFLTTLMEDVHSRDSPVQAFTSEE 120
           GTTGCPLAATLSS +RVLVLERGGVP+GN NLMSQEGFL TLM++ +SRDSPVQAFTSEE
Sbjct: 61  GTTGCPLAATLSSHYRVLVLERGGVPYGNSNLMSQEGFLMTLMDEAYSRDSPVQAFTSEE 120

Query: 121 GVPNARGRILGGSSAINAGFYSRADRAFFQRSHLINWDLTMVNQSYEWVEKEIVFRPNLK 180
           GVPNARGRILGGSSAINAGFYSRADRAFF RSHL NWDLTMVNQSYEWVEK+IVF+PNLK
Sbjct: 121 GVPNARGRILGGSSAINAGFYSRADRAFFHRSHL-NWDLTMVNQSYEWVEKKIVFKPNLK 180

Query: 181 HWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIGGSTFDETGRRHSAADLLSYATPFNIKVA 240
           +WQS VRDGMVEAGI PYNGF+LDHLVGTKIGGSTFDE GRRHSAADLLSYATPFNI+VA
Sbjct: 181 NWQSVVRDGMVEAGINPYNGFTLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIQVA 240

Query: 241 VYATVERILLASTRPLRSSAIGVLYRDEKGAYHHAMLRARGEVLLCAGAIGSPQLLLLSG 300
           VYATVERILL ST     +A+GVLYRD++GAYHHAML ARGE+LL AGAIGSPQLLLLSG
Sbjct: 241 VYATVERILLTSTGQFGRAAVGVLYRDQRGAYHHAMLCARGEILLSAGAIGSPQLLLLSG 300

Query: 301 IGSRSYLSSWGIPVSHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEA 360
           IGSR YLSSWGIPV+HHLP+VGH LYDNPRNGISILAPMPLEQSLIQVAGITESG+YLEA
Sbjct: 301 IGSRPYLSSWGIPVAHHLPFVGHNLYDNPRNGISILAPMPLEQSLIQVAGITESGSYLEA 360

Query: 361 ASNVIPFTSPSRSNFFIRTPSAPVYLNVATLMEKIVGPVSSGSMRLASRDVRVNPVVRFN 420
           ASNVIPFTSP RS FFI+TPSAPVYL VATLMEKI GPVS GSMRLAS DVR NPVVRFN
Sbjct: 361 ASNVIPFTSPPRS-FFIKTPSAPVYLTVATLMEKIAGPVSFGSMRLASTDVRTNPVVRFN 420

Query: 421 YFRKGVDLERCVNGTRKIGEILRSRSMERFKFRDWLGRREFRFVGPVYPEDDEDERQLEL 480
           YF   VDLERCVNG RKIGE+LRSRSME FKFR+  GRREFRFVGPVYP D       E 
Sbjct: 421 YFSNTVDLERCVNGMRKIGEVLRSRSMESFKFRERSGRREFRFVGPVYPAD-------EG 480

Query: 481 ELRRFCRGSVSTIWHYHGGCVMGRVVERNYGVIGMEALRVVDGSTFGVSPGTNPQATLMM 540
            LR FCR SVSTIWHYHGGCV G+VVERNY VIGM+ALRVVDGSTFGVSPGTNPQATLMM
Sbjct: 481 RLREFCRESVSTIWHYHGGCVTGKVVERNYKVIGMKALRVVDGSTFGVSPGTNPQATLMM 540

Query: 541 LGRYVGLQIINENEPRSTATTS 563
           LGRYVGLQIIN+   RS+ATT+
Sbjct: 541 LGRYVGLQIINQR--RSSATTT 551

[Close]

BLAST of Tan0022232 vs. NCBI nr
Match: XP_023514980.1 ((R)-mandelonitrile lyase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 924.1 bits (2387), Expect = 5.9e-265
Identity = 470/562 (83.63%), Postives = 499/562 (88.79%), Query Frame = 0


Query: 1   MTRRIFFYSQFLMFLAIFYGSFSISAHPNPPQHEPAYLKFVSNATEFPVEDYYDYIIVGG 60
           M  RIFFYSQFL+F AIFY S SISA    P   P Y+KFVSNATEFPV+DYYDYIIVGG
Sbjct: 1   MILRIFFYSQFLVFFAIFYASSSISADRRSPWEGPKYMKFVSNATEFPVKDYYDYIIVGG 60

Query: 61  GTTGCPLAATLSSKFRVLVLERGGVPHGNPNLMSQEGFLTTLMEDVHSRDSPVQAFTSEE 120
           GTTGCPLAATLSS +RVLVLERGGVP+GN NLMSQEGFL TLM++ +SRDSPVQAFTSEE
Sbjct: 61  GTTGCPLAATLSSHYRVLVLERGGVPYGNSNLMSQEGFLMTLMDEAYSRDSPVQAFTSEE 120

Query: 121 GVPNARGRILGGSSAINAGFYSRADRAFFQRSHLINWDLTMVNQSYEWVEKEIVFRPNLK 180
           GVPNARGRILGGSSAINAGFYSRADRAFF RSHL NWDLTMVNQSYEWVEK+IVF+PNLK
Sbjct: 121 GVPNARGRILGGSSAINAGFYSRADRAFFHRSHL-NWDLTMVNQSYEWVEKKIVFKPNLK 180

Query: 181 HWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIGGSTFDETGRRHSAADLLSYATPFNIKVA 240
           +WQS VRDGMVEAGI PYNGF+L+HLVGTKIGGSTFDE GRRHSAADLLSYATPFNI+VA
Sbjct: 181 NWQSVVRDGMVEAGINPYNGFTLEHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIQVA 240

Query: 241 VYATVERILLASTRPLRSSAIGVLYRDEKGAYHHAMLRARGEVLLCAGAIGSPQLLLLSG 300
           VYATVERILL ST     +A+GVLYRD++GAYHHAML ARGEVLL AGAIGSPQLLLLSG
Sbjct: 241 VYATVERILLTSTGQFGRAAVGVLYRDQRGAYHHAMLCARGEVLLSAGAIGSPQLLLLSG 300

Query: 301 IGSRSYLSSWGIPVSHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEA 360
           IGSR YLSSWGIPV+HHLP+VGH LYDNPRNGISILAPMPLEQSLIQVAGITESG+YLEA
Sbjct: 301 IGSRPYLSSWGIPVAHHLPFVGHNLYDNPRNGISILAPMPLEQSLIQVAGITESGSYLEA 360

Query: 361 ASNVIPFTSPSRSNFFIRTPSAPVYLNVATLMEKIVGPVSSGSMRLASRDVRVNPVVRFN 420
           ASNVIPFTSP RS FFI+TPSAPVYL VATLMEKI GPVS GSMRLAS DVR NPVVRFN
Sbjct: 361 ASNVIPFTSPPRS-FFIKTPSAPVYLTVATLMEKIAGPVSFGSMRLASTDVRTNPVVRFN 420

Query: 421 YFRKGVDLERCVNGTRKIGEILRSRSMERFKFRDWLGRREFRFVGPVYPEDDEDERQLEL 480
           YF   VDLERCVNG RKIGE+LRSRSME FKFR+  GRREFRFVGPVYP D       E 
Sbjct: 421 YFSNTVDLERCVNGMRKIGEVLRSRSMESFKFRERSGRREFRFVGPVYPAD-------EG 480

Query: 481 ELRRFCRGSVSTIWHYHGGCVMGRVVERNYGVIGMEALRVVDGSTFGVSPGTNPQATLMM 540
            LR FCR SVSTIWHYHGGCV G+VVERNY VIGM+ALRVVDGSTFGVSPGTNPQATLMM
Sbjct: 481 RLREFCRESVSTIWHYHGGCVTGKVVERNYKVIGMKALRVVDGSTFGVSPGTNPQATLMM 540

Query: 541 LGRYVGLQIINENEPRSTATTS 563
           LGRYVGLQIIN+   RS+ATT+
Sbjct: 541 LGRYVGLQIINQR--RSSATTT 551

[Close]

BLAST of Tan0022232 vs. NCBI nr
Match: KGN45431.1 (hypothetical protein Csa_016192 [Cucumis sativus])

HSP 1 Score: 908.7 bits (2347), Expect = 2.6e-260
Identity = 463/561 (82.53%), Postives = 492/561 (87.70%), Query Frame = 0


Query: 1   MTRRIFFYSQFLMFLAIFYGSFSISAHPNPPQHEPAYLKFVSNATEFPVEDYYDYIIVGG 60
           M  RI F+SQ L FL IF  S   SA PN  Q +P YLKFVSNATEFP  DYYDYIIVGG
Sbjct: 1   MESRISFHSQILTFLLIFSAS---SALPNSSQQDPDYLKFVSNATEFPEVDYYDYIIVGG 60

Query: 61  GTTGCPLAATLSSKFRVLVLERGGVPHGNPNLMSQEGFLTTLMEDVHSRDSPVQAFTSEE 120
           GT GCPLAATLSSKFRVLVLERGGVPHGN NLMSQEGFLTTLM+DVHSR+SPVQAFTSEE
Sbjct: 61  GTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTSEE 120

Query: 121 GVPNARGRILGGSSAINAGFYSRADRAFFQRSHLINWDLTMVNQSYEWVEKEIVFRPNLK 180
           GVPNARGRILGGSSAINAGFYSRAD+AFF+ S L  WDL +VNQSYEWVEKEIVF+PNLK
Sbjct: 121 GVPNARGRILGGSSAINAGFYSRADQAFFKNSQL-KWDLAIVNQSYEWVEKEIVFKPNLK 180

Query: 181 HWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIGGSTFDETGRRHSAADLLSYATPFNIKVA 240
           +WQSAVRDGMVEAGI PYNGFSLDH++GTKIGGSTFDE GRRHSAADLLS+ATPFNIKVA
Sbjct: 181 NWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSHATPFNIKVA 240

Query: 241 VYATVERILLASTRPLRSSAIGVLYRDEKGAYHHAMLRARGEVLLCAGAIGSPQLLLLSG 300
           +YA VERILLAST   R  AIGVLYRD+ G YHHAML ARGEV L AGAIGSPQLLLLSG
Sbjct: 241 IYANVERILLASTVKRRPKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQLLLLSG 300

Query: 301 IGSRSYLSSWGIPVSHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEA 360
           IGSR YLSSWGIPV+HHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESG YLEA
Sbjct: 301 IGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLEA 360

Query: 361 ASNVIPFTSPSRSNFFIRTPSAPVYLNVATLMEKIVGPVSSGSMRLASRDVRVNPVVRFN 420
           ASNVIPFTSP RS FFIRTPS PVYL VATLMEKI GPV+SGSMRLAS DVRVNPVVRFN
Sbjct: 361 ASNVIPFTSPPRS-FFIRTPSPPVYLTVATLMEKITGPVASGSMRLASTDVRVNPVVRFN 420

Query: 421 YFRKGVDLERCVNGTRKIGEILRSRSMERFKFRDWLGRREFRFVGPVYPEDDEDERQLEL 480
           YF   VDLERCVNGTRKIGEILRS++ME FKFRDW GR+EFR+VGP YPE ++++    L
Sbjct: 421 YFGNAVDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDED---GL 480

Query: 481 ELRRFCRGSVSTIWHYHGGCVMGRVVERNYGVIGMEALRVVDGSTFGVSPGTNPQATLMM 540
            LR FCR SVSTIWHYHGGCV G+VV+ NY VIGMEALRVVDGS FGVSPGTNPQAT+MM
Sbjct: 481 VLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMM 540

Query: 541 LGRYVGLQIINENEPRSTATT 562
           LGR+VGLQIIN+   RS  TT
Sbjct: 541 LGRHVGLQIINQ---RSAVTT 550

[Close]

BLAST of Tan0022232 vs. ExPASy TrEMBL
Match: A0A6J1KJ63 ((R)-mandelonitrile lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111494473 PE=3 SV=1)

HSP 1 Score: 925.6 bits (2391), Expect = 9.8e-266
Identity = 471/562 (83.81%), Postives = 499/562 (88.79%), Query Frame = 0


Query: 1   MTRRIFFYSQFLMFLAIFYGSFSISAHPNPPQHEPAYLKFVSNATEFPVEDYYDYIIVGG 60
           M  RIFFYSQFL+F AIFY S SISA    P   P Y+KFVSNATEFPV+DYYDYIIVGG
Sbjct: 1   MILRIFFYSQFLVFFAIFYASSSISADRRSPWEGPKYMKFVSNATEFPVKDYYDYIIVGG 60

Query: 61  GTTGCPLAATLSSKFRVLVLERGGVPHGNPNLMSQEGFLTTLMEDVHSRDSPVQAFTSEE 120
           GTTGCPLAATLSS +RVLVLERGGVP+GN NLMSQEGFL TLM++ +SRDSPVQAFTSEE
Sbjct: 61  GTTGCPLAATLSSHYRVLVLERGGVPYGNSNLMSQEGFLMTLMDEAYSRDSPVQAFTSEE 120

Query: 121 GVPNARGRILGGSSAINAGFYSRADRAFFQRSHLINWDLTMVNQSYEWVEKEIVFRPNLK 180
           GVPNARGRILGGSSAINAGFYSRADRAFF RSHL NWDLTMVNQSYEWVEK+IVF+PNLK
Sbjct: 121 GVPNARGRILGGSSAINAGFYSRADRAFFHRSHL-NWDLTMVNQSYEWVEKKIVFKPNLK 180

Query: 181 HWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIGGSTFDETGRRHSAADLLSYATPFNIKVA 240
           +WQS VRDGMVEAGI PYNGF+LDHLVGTKIGGSTFDE GRRHSAADLLSYATPFNI+VA
Sbjct: 181 NWQSVVRDGMVEAGINPYNGFTLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIQVA 240

Query: 241 VYATVERILLASTRPLRSSAIGVLYRDEKGAYHHAMLRARGEVLLCAGAIGSPQLLLLSG 300
           VYATVERILL ST     +A+GVLYRD++GAYHHA+L ARGEVLL AGAIGSPQLLLLSG
Sbjct: 241 VYATVERILLTSTGQFGRAAVGVLYRDQRGAYHHAILCARGEVLLSAGAIGSPQLLLLSG 300

Query: 301 IGSRSYLSSWGIPVSHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEA 360
           IGSR YLSSWGIPV+HHLP+VGH LYDNPRNGISILAPMPLEQSLIQVAGITESG+YLEA
Sbjct: 301 IGSRPYLSSWGIPVAHHLPFVGHNLYDNPRNGISILAPMPLEQSLIQVAGITESGSYLEA 360

Query: 361 ASNVIPFTSPSRSNFFIRTPSAPVYLNVATLMEKIVGPVSSGSMRLASRDVRVNPVVRFN 420
           ASNVIPFTSP RS FFI+TPSAPVYL VATLMEKI GPVS GSMRLAS DVR NPVVRFN
Sbjct: 361 ASNVIPFTSPPRS-FFIKTPSAPVYLTVATLMEKIAGPVSFGSMRLASTDVRTNPVVRFN 420

Query: 421 YFRKGVDLERCVNGTRKIGEILRSRSMERFKFRDWLGRREFRFVGPVYPEDDEDERQLEL 480
           YF   VDLERCVNG RKIGE+LRSRSME FKFRD  GRREFRFVGPVYP D       E 
Sbjct: 421 YFSNTVDLERCVNGMRKIGEVLRSRSMESFKFRDRSGRREFRFVGPVYPAD-------EG 480

Query: 481 ELRRFCRGSVSTIWHYHGGCVMGRVVERNYGVIGMEALRVVDGSTFGVSPGTNPQATLMM 540
            LR FCR SVSTIWHYHGGCV G+VVERNY VIGM+ALRVVDGSTFGVSPGTNPQATLMM
Sbjct: 481 RLREFCRESVSTIWHYHGGCVTGKVVERNYKVIGMKALRVVDGSTFGVSPGTNPQATLMM 540

Query: 541 LGRYVGLQIINENEPRSTATTS 563
           LGRYVGLQIIN+   RS+ATT+
Sbjct: 541 LGRYVGLQIINQR--RSSATTT 551

[Close]

BLAST of Tan0022232 vs. ExPASy TrEMBL
Match: A0A6J1HN57 ((R)-mandelonitrile lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111464460 PE=3 SV=1)

HSP 1 Score: 925.2 bits (2390), Expect = 1.3e-265
Identity = 470/562 (83.63%), Postives = 499/562 (88.79%), Query Frame = 0


Query: 1   MTRRIFFYSQFLMFLAIFYGSFSISAHPNPPQHEPAYLKFVSNATEFPVEDYYDYIIVGG 60
           M  RIFFYSQFL+F AIFY S SISA    P   P Y+KFVSNATEFPV+DYYDYIIVGG
Sbjct: 1   MILRIFFYSQFLVFFAIFYASSSISADRRSPWEGPKYMKFVSNATEFPVKDYYDYIIVGG 60

Query: 61  GTTGCPLAATLSSKFRVLVLERGGVPHGNPNLMSQEGFLTTLMEDVHSRDSPVQAFTSEE 120
           GTTGCPLAATLSS +RVLVLERGGVP+GN NLMSQEGFL TLM++ +SRDSPVQAFTSEE
Sbjct: 61  GTTGCPLAATLSSHYRVLVLERGGVPYGNSNLMSQEGFLMTLMDEAYSRDSPVQAFTSEE 120

Query: 121 GVPNARGRILGGSSAINAGFYSRADRAFFQRSHLINWDLTMVNQSYEWVEKEIVFRPNLK 180
           GVPNARGRILGGSSAINAGFYSRADRAFF RSHL NWDLTMVNQSYEWVEK+IVF+PNLK
Sbjct: 121 GVPNARGRILGGSSAINAGFYSRADRAFFHRSHL-NWDLTMVNQSYEWVEKKIVFKPNLK 180

Query: 181 HWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIGGSTFDETGRRHSAADLLSYATPFNIKVA 240
           +WQS VRDGMVEAGI PYNGF+LDHLVGTKIGGSTFDE GRRHSAADLLSYATPFNI+VA
Sbjct: 181 NWQSVVRDGMVEAGINPYNGFTLDHLVGTKIGGSTFDEMGRRHSAADLLSYATPFNIQVA 240

Query: 241 VYATVERILLASTRPLRSSAIGVLYRDEKGAYHHAMLRARGEVLLCAGAIGSPQLLLLSG 300
           VYATVERILL ST     +A+GVLYRD++GAYHHAML ARGE+LL AGAIGSPQLLLLSG
Sbjct: 241 VYATVERILLTSTGQFGRAAVGVLYRDQRGAYHHAMLCARGEILLSAGAIGSPQLLLLSG 300

Query: 301 IGSRSYLSSWGIPVSHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEA 360
           IGSR YLSSWGIPV+HHLP+VGH LYDNPRNGISILAPMPLEQSLIQVAGITESG+YLEA
Sbjct: 301 IGSRPYLSSWGIPVAHHLPFVGHNLYDNPRNGISILAPMPLEQSLIQVAGITESGSYLEA 360

Query: 361 ASNVIPFTSPSRSNFFIRTPSAPVYLNVATLMEKIVGPVSSGSMRLASRDVRVNPVVRFN 420
           ASNVIPFTSP RS FFI+TPSAPVYL VATLMEKI GPVS GSMRLAS DVR NPVVRFN
Sbjct: 361 ASNVIPFTSPPRS-FFIKTPSAPVYLTVATLMEKIAGPVSFGSMRLASTDVRTNPVVRFN 420

Query: 421 YFRKGVDLERCVNGTRKIGEILRSRSMERFKFRDWLGRREFRFVGPVYPEDDEDERQLEL 480
           YF   VDLERCVNG RKIGE+LRSRSME FKFR+  GRREFRFVGPVYP D       E 
Sbjct: 421 YFSNTVDLERCVNGMRKIGEVLRSRSMESFKFRERSGRREFRFVGPVYPAD-------EG 480

Query: 481 ELRRFCRGSVSTIWHYHGGCVMGRVVERNYGVIGMEALRVVDGSTFGVSPGTNPQATLMM 540
            LR FCR SVSTIWHYHGGCV G+VVERNY VIGM+ALRVVDGSTFGVSPGTNPQATLMM
Sbjct: 481 RLREFCRESVSTIWHYHGGCVTGKVVERNYKVIGMKALRVVDGSTFGVSPGTNPQATLMM 540

Query: 541 LGRYVGLQIINENEPRSTATTS 563
           LGRYVGLQIIN+   RS+ATT+
Sbjct: 541 LGRYVGLQIINQR--RSSATTT 551

[Close]

BLAST of Tan0022232 vs. ExPASy TrEMBL
Match: A0A0A0KCB1 (GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G447930 PE=3 SV=1)

HSP 1 Score: 908.7 bits (2347), Expect = 1.2e-260
Identity = 463/561 (82.53%), Postives = 492/561 (87.70%), Query Frame = 0


Query: 1   MTRRIFFYSQFLMFLAIFYGSFSISAHPNPPQHEPAYLKFVSNATEFPVEDYYDYIIVGG 60
           M  RI F+SQ L FL IF  S   SA PN  Q +P YLKFVSNATEFP  DYYDYIIVGG
Sbjct: 1   MESRISFHSQILTFLLIFSAS---SALPNSSQQDPDYLKFVSNATEFPEVDYYDYIIVGG 60

Query: 61  GTTGCPLAATLSSKFRVLVLERGGVPHGNPNLMSQEGFLTTLMEDVHSRDSPVQAFTSEE 120
           GT GCPLAATLSSKFRVLVLERGGVPHGN NLMSQEGFLTTLM+DVHSR+SPVQAFTSEE
Sbjct: 61  GTAGCPLAATLSSKFRVLVLERGGVPHGNSNLMSQEGFLTTLMDDVHSRNSPVQAFTSEE 120

Query: 121 GVPNARGRILGGSSAINAGFYSRADRAFFQRSHLINWDLTMVNQSYEWVEKEIVFRPNLK 180
           GVPNARGRILGGSSAINAGFYSRAD+AFF+ S L  WDL +VNQSYEWVEKEIVF+PNLK
Sbjct: 121 GVPNARGRILGGSSAINAGFYSRADQAFFKNSQL-KWDLAIVNQSYEWVEKEIVFKPNLK 180

Query: 181 HWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIGGSTFDETGRRHSAADLLSYATPFNIKVA 240
           +WQSAVRDGMVEAGI PYNGFSLDH++GTKIGGSTFDE GRRHSAADLLS+ATPFNIKVA
Sbjct: 181 NWQSAVRDGMVEAGIIPYNGFSLDHVMGTKIGGSTFDELGRRHSAADLLSHATPFNIKVA 240

Query: 241 VYATVERILLASTRPLRSSAIGVLYRDEKGAYHHAMLRARGEVLLCAGAIGSPQLLLLSG 300
           +YA VERILLAST   R  AIGVLYRD+ G YHHAML ARGEV L AGAIGSPQLLLLSG
Sbjct: 241 IYANVERILLASTVKRRPKAIGVLYRDQIGTYHHAMLHARGEVFLSAGAIGSPQLLLLSG 300

Query: 301 IGSRSYLSSWGIPVSHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEA 360
           IGSR YLSSWGIPV+HHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESG YLEA
Sbjct: 301 IGSRPYLSSWGIPVAHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGTYLEA 360

Query: 361 ASNVIPFTSPSRSNFFIRTPSAPVYLNVATLMEKIVGPVSSGSMRLASRDVRVNPVVRFN 420
           ASNVIPFTSP RS FFIRTPS PVYL VATLMEKI GPV+SGSMRLAS DVRVNPVVRFN
Sbjct: 361 ASNVIPFTSPPRS-FFIRTPSPPVYLTVATLMEKITGPVASGSMRLASTDVRVNPVVRFN 420

Query: 421 YFRKGVDLERCVNGTRKIGEILRSRSMERFKFRDWLGRREFRFVGPVYPEDDEDERQLEL 480
           YF   VDLERCVNGTRKIGEILRS++ME FKFRDW GR+EFR+VGP YPE ++++    L
Sbjct: 421 YFGNAVDLERCVNGTRKIGEILRSQAMEGFKFRDWFGRKEFRYVGPAYPEKEDED---GL 480

Query: 481 ELRRFCRGSVSTIWHYHGGCVMGRVVERNYGVIGMEALRVVDGSTFGVSPGTNPQATLMM 540
            LR FCR SVSTIWHYHGGCV G+VV+ NY VIGMEALRVVDGS FGVSPGTNPQAT+MM
Sbjct: 481 VLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVIGMEALRVVDGSIFGVSPGTNPQATVMM 540

Query: 541 LGRYVGLQIINENEPRSTATT 562
           LGR+VGLQIIN+   RS  TT
Sbjct: 541 LGRHVGLQIINQ---RSAVTT 550

[Close]

BLAST of Tan0022232 vs. ExPASy TrEMBL
Match: A0A1S3CHZ4 ((R)-mandelonitrile lyase-like OS=Cucumis melo OX=3656 GN=LOC103500635 PE=3 SV=1)

HSP 1 Score: 905.2 bits (2338), Expect = 1.4e-259
Identity = 459/561 (81.82%), Postives = 490/561 (87.34%), Query Frame = 0


Query: 1   MTRRIFFYSQFLMFLAIFYGSFSISAHPNPPQHEPAYLKFVSNATEFPVEDYYDYIIVGG 60
           M  RIFF+SQ L FL IF  S   SA PN  Q +P YLKFVSNATEFP  DYYDYIIVGG
Sbjct: 19  MASRIFFHSQILTFLLIFSAS---SALPNSSQKDPDYLKFVSNATEFPKVDYYDYIIVGG 78

Query: 61  GTTGCPLAATLSSKFRVLVLERGGVPHGNPNLMSQEGFLTTLMEDVHSRDSPVQAFTSEE 120
           GT GCPLAATLS+KFRVLVLERGG PHGN NLMSQEGFLTTLM+DVHSR SPVQAFTSEE
Sbjct: 79  GTAGCPLAATLSNKFRVLVLERGGFPHGNSNLMSQEGFLTTLMDDVHSRYSPVQAFTSEE 138

Query: 121 GVPNARGRILGGSSAINAGFYSRADRAFFQRSHLINWDLTMVNQSYEWVEKEIVFRPNLK 180
           GVPNARGRILGGSSAINAGFYSRAD+AFF+ S L  WDL +VNQSYEWVEKEIVF+PNLK
Sbjct: 139 GVPNARGRILGGSSAINAGFYSRADQAFFKNSQL-KWDLAIVNQSYEWVEKEIVFKPNLK 198

Query: 181 HWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIGGSTFDETGRRHSAADLLSYATPFNIKVA 240
           +WQSAVRDGMVEAGI PYNGFSLDH++GTKIGGSTFDE GRRHSAADLLSYATPFNIKVA
Sbjct: 199 NWQSAVRDGMVEAGITPYNGFSLDHVIGTKIGGSTFDELGRRHSAADLLSYATPFNIKVA 258

Query: 241 VYATVERILLASTRPLRSSAIGVLYRDEKGAYHHAMLRARGEVLLCAGAIGSPQLLLLSG 300
           +YATVERILL ST   +  AIGVLYRD+ G YHHAML ARGEV L AGAIGSPQLLLLSG
Sbjct: 259 IYATVERILLGSTVKRKPKAIGVLYRDQIGKYHHAMLHARGEVFLSAGAIGSPQLLLLSG 318

Query: 301 IGSRSYLSSWGIPVSHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEA 360
           IGSR YLSSWGIPV+HHLPYVGHYLYDNPR GISILAPMPLEQSLIQVAGITESG YLEA
Sbjct: 319 IGSRPYLSSWGIPVAHHLPYVGHYLYDNPRTGISILAPMPLEQSLIQVAGITESGTYLEA 378

Query: 361 ASNVIPFTSPSRSNFFIRTPSAPVYLNVATLMEKIVGPVSSGSMRLASRDVRVNPVVRFN 420
           ASNVIPFT+P RS FFIRTPS PVYL VATLMEKI GPV+SG+MRLAS DVRVNPVVRFN
Sbjct: 379 ASNVIPFTTPPRS-FFIRTPSPPVYLTVATLMEKITGPVASGAMRLASTDVRVNPVVRFN 438

Query: 421 YFRKGVDLERCVNGTRKIGEILRSRSMERFKFRDWLGRREFRFVGPVYPEDDEDERQLEL 480
           YF   VDLERCVNGTRKIG+ILRS++ME FKFRDW GR+EFRFVGP YPED +++    L
Sbjct: 439 YFGNAVDLERCVNGTRKIGDILRSQAMEGFKFRDWFGRKEFRFVGPAYPEDGDED---GL 498

Query: 481 ELRRFCRGSVSTIWHYHGGCVMGRVVERNYGVIGMEALRVVDGSTFGVSPGTNPQATLMM 540
            LR FCR SVSTIWHYHGGCV G+VV+ NY V+GMEALRVVDGS FGVSPGTNPQAT+MM
Sbjct: 499 VLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVMGMEALRVVDGSIFGVSPGTNPQATVMM 558

Query: 541 LGRYVGLQIINENEPRSTATT 562
           LGR+VGLQIIN+   RS  TT
Sbjct: 559 LGRHVGLQIINQ---RSAVTT 568

[Close]

BLAST of Tan0022232 vs. ExPASy TrEMBL
Match: A0A5D3C0K1 ((R)-mandelonitrile lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G001740 PE=3 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 3.0e-259
Identity = 459/561 (81.82%), Postives = 489/561 (87.17%), Query Frame = 0


Query: 1   MTRRIFFYSQFLMFLAIFYGSFSISAHPNPPQHEPAYLKFVSNATEFPVEDYYDYIIVGG 60
           M  RIFF+SQ L FL IF  S   SA PN  Q +P YLKFVSNATEFP  DYYDYIIVGG
Sbjct: 19  MASRIFFHSQILTFLLIFSAS---SALPNSSQKDPDYLKFVSNATEFPKVDYYDYIIVGG 78

Query: 61  GTTGCPLAATLSSKFRVLVLERGGVPHGNPNLMSQEGFLTTLMEDVHSRDSPVQAFTSEE 120
           GT GCPLAATLS+KFRVLVLERGG PHGN NLMSQEGFLTTLM+DVHSR SPVQAFTSEE
Sbjct: 79  GTAGCPLAATLSNKFRVLVLERGGFPHGNSNLMSQEGFLTTLMDDVHSRYSPVQAFTSEE 138

Query: 121 GVPNARGRILGGSSAINAGFYSRADRAFFQRSHLINWDLTMVNQSYEWVEKEIVFRPNLK 180
           GVPNARGRILGGSSAINAGFYSRAD+AFF+ S L  WDL +VNQSYEWVEKEIVF+PNLK
Sbjct: 139 GVPNARGRILGGSSAINAGFYSRADQAFFKNSQL-KWDLAIVNQSYEWVEKEIVFKPNLK 198

Query: 181 HWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIGGSTFDETGRRHSAADLLSYATPFNIKVA 240
           +WQSAVRDGMVEAGI PYNGFSLDH++GTKIGGSTFDE GRRHSAADLLSYATPFNIKVA
Sbjct: 199 NWQSAVRDGMVEAGITPYNGFSLDHVIGTKIGGSTFDELGRRHSAADLLSYATPFNIKVA 258

Query: 241 VYATVERILLASTRPLRSSAIGVLYRDEKGAYHHAMLRARGEVLLCAGAIGSPQLLLLSG 300
           +YATVERILL ST   +  AIGVLYRD+ G YHHAML ARGEV L AGAIGSPQLLLLSG
Sbjct: 259 IYATVERILLGSTVKRKPKAIGVLYRDQIGKYHHAMLHARGEVFLSAGAIGSPQLLLLSG 318

Query: 301 IGSRSYLSSWGIPVSHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEA 360
           IGSR YLSSWGIPV HHLPYVGHYLYDNPR GISILAPMPLEQSLIQVAGITESG YLEA
Sbjct: 319 IGSRPYLSSWGIPVVHHLPYVGHYLYDNPRTGISILAPMPLEQSLIQVAGITESGTYLEA 378

Query: 361 ASNVIPFTSPSRSNFFIRTPSAPVYLNVATLMEKIVGPVSSGSMRLASRDVRVNPVVRFN 420
           ASNVIPFT+P RS FFIRTPS PVYL VATLMEKI GPV+SG+MRLAS DVRVNPVVRFN
Sbjct: 379 ASNVIPFTTPPRS-FFIRTPSPPVYLTVATLMEKITGPVASGAMRLASTDVRVNPVVRFN 438

Query: 421 YFRKGVDLERCVNGTRKIGEILRSRSMERFKFRDWLGRREFRFVGPVYPEDDEDERQLEL 480
           YF   VDLERCVNGTRKIG+ILRS++ME FKFRDW GR+EFRFVGP YPED +++    L
Sbjct: 439 YFGNAVDLERCVNGTRKIGDILRSQAMEGFKFRDWFGRKEFRFVGPAYPEDGDED---GL 498

Query: 481 ELRRFCRGSVSTIWHYHGGCVMGRVVERNYGVIGMEALRVVDGSTFGVSPGTNPQATLMM 540
            LR FCR SVSTIWHYHGGCV G+VV+ NY V+GMEALRVVDGS FGVSPGTNPQAT+MM
Sbjct: 499 VLREFCRQSVSTIWHYHGGCVAGKVVDSNYRVMGMEALRVVDGSIFGVSPGTNPQATVMM 558

Query: 541 LGRYVGLQIINENEPRSTATT 562
           LGR+VGLQIIN+   RS  TT
Sbjct: 559 LGRHVGLQIINQ---RSAVTT 568

[Close]

BLAST of Tan0022232 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 721.8 bits (1862), Expect = 4.1e-208
Identity = 351/521 (67.37%), Postives = 427/521 (81.96%), Query Frame = 0


Query: 35  PAYLKFVSNATEFPVEDYYDYIIVGGGTTGCPLAATLSSKFRVLVLERGGVPHGNPNLMS 94
           P +++F+SNAT+F  EDYYDYIIVGGGT GCPLAATLS  FRVL+LERGGVP+  PN+MS
Sbjct: 36  PGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPNVMS 95

Query: 95  QEGFLTTLMEDVHSRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADRAFFQRSHL 154
            +GFLTTL  DV++ DSP Q+F SEEGVPNARGR+LGGSSAINAGFYSRAD+ FF+ S L
Sbjct: 96  HDGFLTTL-TDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENSGL 155

Query: 155 INWDLTMVNQSYEWVEKEIVFRPNLKHWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIGGS 214
           + WDL+ VNQSYEWVE+ IVFRP L+ WQ+A+RD ++E G+ P+NGF+L+H VGTKIGGS
Sbjct: 156 V-WDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGGS 215

Query: 215 TFDETGRRHSAADLLSYATPFNIKVAVYATVERILLASTRPLRS---SAIGVLYRDEKGA 274
           TFD TGRRHS+ADLL YA   NI+VAVYATVER+LLAS+  +     SAIGV+YRD+ G 
Sbjct: 216 TFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLGR 275

Query: 275 YHHAMLRARGEVLLCAGAIGSPQLLLLSGIGSRSYLSSWGIPVSHHLPYVGHYLYDNPRN 334
           +HHA++R RGEV+L AGA+GSPQLL LSGIG RSYLS+WGIPV+   P+VG ++YDNPRN
Sbjct: 276 FHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPRN 335

Query: 335 GISILAPMPLEQSLIQVAGITESGAYLEAASNVIPFTSPSRSNFFIRTPSAPVYLNVATL 394
           GISI+ P+P+E SLIQV G+TE GA+LEAASNVIPF SP  S  FIR P++P+Y+ V T+
Sbjct: 336 GISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHS-VFIRAPASPLYVPVTTI 395

Query: 395 MEKIVGPVSSGSMRLASRDVRVNPVVRFNYFRKGVDLERCVNGTRKIGEILRSRSMERFK 454
           MEKI+GPVS G +RLAS DVR+NPVVRFNYF    DLERCVNGTRKIGEILRSR+M+ F 
Sbjct: 396 MEKILGPVSIGLLRLASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQDFM 455

Query: 455 FRDWLGRREFRFVGPVYPEDDEDERQLELELRRFCRGSVSTIWHYHGGCVMGRVVERNYG 514
            R+W G R FRFVG   P D  +    +L +  FCR +VSTIWHYHGG V+G+VV+ +  
Sbjct: 456 IREWFGNRRFRFVGAPLPVDQSN----DLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLK 515

Query: 515 VIGMEALRVVDGSTFGVSPGTNPQATLMMLGRYVGLQIINE 553
           VIG+ +LR+VDGSTF +SPGTNPQATLMMLGRY+GL+++ E
Sbjct: 516 VIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRE 549

[Close]

BLAST of Tan0022232 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 462.2 bits (1188), Expect = 5.9e-130
Identity = 244/541 (45.10%), Postives = 333/541 (61.55%), Query Frame = 0


Query: 40  FVSNATEFPVEDYYDYIIVGGGTTGCPLAATLSSKFRVLVLERGGVPHGNPNLMSQEGFL 99
           F+ +AT  P   YYDYII+GGGT GCPLAATLS    VL+LERG  P+ NPN+     F 
Sbjct: 33  FMRDATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLERGDSPYNNPNITRLSAFG 92

Query: 100 TTLMEDVHSRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADRAFFQRSHLINWDL 159
             L +   S  SP Q F SE+GV NAR R+LGG SA+NAGFY+RA   + +    + WD 
Sbjct: 93  AALSD--LSESSPSQRFVSEDGVINARARVLGGGSALNAGFYTRAGTKYVRN---MGWDG 152

Query: 160 TMVNQSYEWVEKEIVFRPNLKHWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIGGSTFDET 219
            + N+SY+WVE ++ F+P +  WQ+AVRDG++EAGI P NGF+ DH+ GTK GG+ FD  
Sbjct: 153 ALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPNNGFTYDHINGTKFGGTIFDRN 212

Query: 220 GRRHSAADLLSYATPFNIKVAVYATVERILLASTRPLRSSAIGVLYRDEKGAYHHAMLR- 279
           G RH+AADLL YA P  I V ++ATV RIL  +    +  A GV+YRD  G  H A L+ 
Sbjct: 213 GNRHTAADLLEYADPKGITVLLHATVHRILFRTRGTTKPIANGVVYRDRTGQAHRAYLKE 272

Query: 280 -ARGEVLLCAGAIGSPQLLLLSGIGSRSYLSSWGIPVSHHLPYVGHYLYDNPRNGISILA 339
            A  E++L AG +GSPQLL+LSG+G  + L +  I V    P+VG  +YDNP N + + +
Sbjct: 273 GALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQGMYDNPMNAVFVPS 332

Query: 340 PMPLEQSLIQVAGITESGAYLEAASN----------------------VIPFTSPSRSNF 399
           P+P+E SLI+V GIT  G Y+EAA                          P  +   SN 
Sbjct: 333 PVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSGSSSTRDYYAMFSPRATLLESNS 392

Query: 400 FIRTPSAPVYLNVATLMEKIVGPVSSGSMRLASRDVRVNPVVRFNYFRKGVDLERCVNGT 459
             +  SA  +     L+EK++GP+S+G + L +R+ + NP+V FNYF+   DL+RCV G 
Sbjct: 393 MTKLSSAQPFQG-GFLLEKVMGPLSTGHLELKTRNPKDNPIVTFNYFQHPDDLKRCVRGI 452

Query: 460 RKIGEILRSRSMERFKFRD----WLGRREFRFVGPVYPEDDEDERQLELELRRFCRGSVS 519
           + I  +++S++  R+K+ D    +L          + P        L      FC+ +V+
Sbjct: 453 QTIERVVQSKAFSRYKYADVSFEYLLNLTASTPVNLRPPRSGPGASLPPSAEEFCQHTVT 512

Query: 520 TIWHYHGGCVMGRVVERNYGVIGMEALRVVDGSTFGVSPGTNPQATLMMLGRYVGLQIIN 553
           TIWHYHGGCV+GRVV+ +Y VIG++ LRV+D ST G  PGTNPQAT+MMLGRY+G++I+ 
Sbjct: 513 TIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTVGYCPGTNPQATVMMLGRYMGVKILR 567

[Close]

BLAST of Tan0022232 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 445.7 bits (1145), Expect = 5.7e-125
Identity = 255/577 (44.19%), Postives = 344/577 (59.62%), Query Frame = 0


Query: 13  MFLAIFYGSFSISAHPNPPQHEPAYLKFVSNATEFPVEDYYDYIIVGGGTTGCPLAATLS 72
           +F  IF  ++   A  +P   +  Y  F+ +AT  P+   +DYII+GGGT+GC LAATLS
Sbjct: 8   LFRKIFIITYIFHASCSP--DKAGYYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLS 67

Query: 73  SKFRVLVLERGGVPHGNPNLMSQEGFLTTLMEDVHSRDSPVQAFTSEEGVPNARGRILGG 132
               VLVLERGG P+ NP     E F TTL     S  S  Q F SE+GV N R R+LGG
Sbjct: 68  QNASVLVLERGGAPYDNPTATDIENFATTLSNT--SPKSWSQLFISEDGVYNTRARVLGG 127

Query: 133 SSAINAGFYSRADRAFFQRSHLINWDLTMVNQSYEWVEKEIVFRPNLKHWQSAVRDGMVE 192
            S +NAGFY+RA   + + +    W    V  +YEWVEK++ F+P +  WQ+A +DG++E
Sbjct: 128 GSVLNAGFYTRAGDEYVKET---EWKTDEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLE 187

Query: 193 AGIRPYNGFSLDHLVGTKIGGSTFDETGRRHSAADLLSYATPFNIKVAVYATVERILLAS 252
           AG  PYNGF+ DH+ GTKIGG+ FD  G RH+AADLL YA P NI V ++A+V +IL  +
Sbjct: 188 AGEFPYNGFTYDHIYGTKIGGTIFDRAGHRHTAADLLEYANPGNIVVYLHASVHKILFTT 247

Query: 253 TRPLRSSAIGVLYRDEKGAYHHAML--RARGEVLLCAGAIGSPQLLLLSGIGSRSYLSSW 312
               R  A GV+++D  G  H A L   +  EV+L AGAIGSPQLL+LSGIG  ++L++ 
Sbjct: 248 KGRPRPKAYGVIFQDANGVLHKAELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAH 307

Query: 313 GI-PVSHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAGITESGAYLEAASNVI---- 372
           GI P+    P VG  + DNP N I I +P P+E SLIQV GIT+  +Y+E AS VI    
Sbjct: 308 GIKPLVLDHPMVGQGMGDNPMNAIFIPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYS 367

Query: 373 ----------------------PFTSPSRSNFFIRTPSAPV--YLNVAT----LMEKIVG 432
                                   TSP+ S   I     P+   LN  T    +++KI G
Sbjct: 368 WTRRFFDGVLNYLNEMQTSRTTSTTSPTLSTQSITDFFNPINPLLNATTRAGLILQKIAG 427

Query: 433 PVSSGSMRLASRDVRVNPVVRFNYFRKGVDLERCVNGTRKIGEILRSRSMERFKFRDWLG 492
           P+S G + L + +   NP VRFNY+++  DL+ CV G   I +++ S++  +FK+ D   
Sbjct: 428 PISRGHLELRNTNPDDNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAFSKFKYPDATI 487

Query: 493 RREFRFVGPVYPEDDEDERQLELELRRFCRGSVSTIWHYHGGCVMGRVVERNYGVIGMEA 552
                 +  V              LR+FC  +V TIWHYHGGC +GRVV++NY V+G+++
Sbjct: 488 HGLLDLMLSVPTNLRPRHITSMFNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDS 547

Query: 553 LRVVDGSTFGVSPGTNPQATLMMLGRYVGLQIINENE 555
           LRV+DGSTF  SPGTNPQAT+MMLGRY+G +I+ E E
Sbjct: 548 LRVIDGSTFLKSPGTNPQATVMMLGRYMGQRILQERE 577

[Close]

BLAST of Tan0022232 vs. TAIR 10
Match: AT5G51950.2 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 440.3 bits (1131), Expect = 2.4e-123
Identity = 248/549 (45.17%), Postives = 332/549 (60.47%), Query Frame = 0


Query: 41  VSNATEFPVEDYYDYIIVGGGTTGCPLAATLSSKFRVLVLERGGVPHGNPNLMSQEGFLT 100
           + +AT  P+   +DYII+GGGT+GC LAATLS    VLVLERGG P+ NP     E F T
Sbjct: 1   MKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAPYDNPTATDIENFAT 60

Query: 101 TLMEDVHSRDSPVQAFTSEEGVPNARGRILGGSSAINAGFYSRADRAFFQRSHLINWDLT 160
           TL     S  S  Q F SE+GV N R R+LGG S +NAGFY+RA   + + +    W   
Sbjct: 61  TLSNT--SPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDEYVKET---EWKTD 120

Query: 161 MVNQSYEWVEKEIVFRPNLKHWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIGGSTFDETG 220
            V  +YEWVEK++ F+P +  WQ+A +DG++EAG  PYNGF+ DH+ GTKIGG+ FD  G
Sbjct: 121 EVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYGTKIGGTIFDRAG 180

Query: 221 RRHSAADLLSYATPFNIKVAVYATVERILLASTRPLRSSAIGVLYRDEKGAYHHAML--R 280
            RH+AADLL YA P NI V ++A+V +IL  +    R  A GV+++D  G  H A L   
Sbjct: 181 HRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDANGVLHKAELEKN 240

Query: 281 ARGEVLLCAGAIGSPQLLLLSGIGSRSYLSSWGI-PVSHHLPYVGHYLYDNPRNGISILA 340
           +  EV+L AGAIGSPQLL+LSGIG  ++L++ GI P+    P VG  + DNP N I I +
Sbjct: 241 SMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGMGDNPMNAIFIPS 300

Query: 341 PMPLEQSLIQVAGITESGAYLEAASNVI--------------------------PFTSPS 400
           P P+E SLIQV GIT+  +Y+E AS VI                            TSP+
Sbjct: 301 PTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEMQTSRTTSTTSPT 360

Query: 401 RSNFFIRTPSAPV--YLNVAT----LMEKIVGPVSSGSMRLASRDVRVNPVVRFNYFRKG 460
            S   I     P+   LN  T    +++KI GP+S G + L + +   NP VRFNY+++ 
Sbjct: 361 LSTQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELRNTNPDDNPSVRFNYYQEP 420

Query: 461 VDLERCVNGTRKIGEILRSRSMERFKFRDWLGRREFRFVGPVYPEDDEDERQLELELRRF 520
            DL+ CV G   I +++ S++  +FK+ D         +  V              LR+F
Sbjct: 421 EDLQICVEGINTIIKVINSKAFSKFKYPDATIHGLLDLMLSVPTNLRPRHITSMFNLRQF 480

Query: 521 CRGSVSTIWHYHGGCVMGRVVERNYGVIGMEALRVVDGSTFGVSPGTNPQATLMMLGRYV 555
           C  +V TIWHYHGGC +GRVV++NY V+G+++LRV+DGSTF  SPGTNPQAT+MMLGRY+
Sbjct: 481 CIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPGTNPQATVMMLGRYM 540

[Close]

BLAST of Tan0022232 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 439.9 bits (1130), Expect = 3.1e-123
Identity = 241/534 (45.13%), Postives = 329/534 (61.61%), Query Frame = 0


Query: 53  YDYIIVGGGTTGCPLAATLSSKFRVLVLERGGVPHGNPNLMSQEGFLTTLMEDVHSRDSP 112
           YDYI++GGGT GCPLAATLS  F VLVLERGGVP  N N+     F   L +   S  S 
Sbjct: 63  YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRNFHIGLAD--ISASSA 122

Query: 113 VQAFTSEEGVPNARGRILGGSSAINAGFYSRADRAFFQRSHLINWDLTMVNQSYEWVEKE 172
            QAF S +GV NAR R+LGG S INAGFYSRAD AF +R+    WD  +V +SY WVE+E
Sbjct: 123 SQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRA---GWDPKLVKESYPWVERE 182

Query: 173 IVFRPNLKHWQSAVRDGMVEAGIRPYNGFSLDHLVGTKIGGSTFDETGRRHSAADLLSYA 232
           IV +P L  WQ A+RD ++E G+RP+NGF+ DH+ GTKIGG+ FD  GRRH+AA+LL+YA
Sbjct: 183 IVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAYA 242

Query: 233 TPFNIKVAVYATVERILLASTRPLRSSAIGVLYRDEKGAYHHAML--RARGEVLLCAGAI 292
            P  ++V +YATV++I+   T   R    GV+++DEKG  H A+L  R   EV+L +GAI
Sbjct: 243 NPQKLRVLIYATVQKIVF-DTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAI 302

Query: 293 GSPQLLLLSGIGSRSYLSSWGIPVSHHLPYVGHYLYDNPRNGISILAPMPLEQSLIQVAG 352
           GSPQ+L+LSGIG +  L    IPV     +VG  + DNP N I + +  P+EQSLIQ  G
Sbjct: 303 GSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQTVG 362

Query: 353 ITESGAYLEAAS-------------------NVIPFTSPSRSNFFIRTPSAPVYL----- 412
           IT+ G Y+EA++                   N +  T P++     R  +   Y+     
Sbjct: 363 ITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQR---RPEATQAYITRNKY 422

Query: 413 ------NVATLMEKIVGPVSSGSMRLASRDVRVNPVVRFNYFRKGVDLERCVNGTRKIGE 472
                 N + ++EK+  P+S G + L + +V  NP V FNYF+  VDL+RCV   R + +
Sbjct: 423 QLHEAFNGSFILEKLAYPISRGHLSLVNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVSK 482

Query: 473 ILRSRSMERFKFRDWLGRREFRFVGPVYPEDDEDERQLE--LELRRFCRGSVSTIWHYHG 532
           ++ S     +   D     +   +  V    +   +QL     + +FC+ +V TIWHYHG
Sbjct: 483 VVTSNRFLNYTQCDKQNVHKMLSLS-VKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHG 542

Query: 533 GCVMGRVVERNYGVIGMEALRVVDGSTFGVSPGTNPQATLMMLGRYVGLQIINE 553
           GC++G+VV  N  V+G++ LRV+DGSTF  SPGTNPQAT+MM+GRY+G++I+ E
Sbjct: 543 GCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRE 586

The following BLAST results are available for this feature:

BLAST of Tan0022232 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SSM2	5.8e-207	67.37	(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ...	[more]
P52707	5.7e-138	47.84	(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1	[more]
P52706	1.3e-137	47.22	(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1	[more]
Q945K2	4.9e-137	47.14	(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1	[more]
O24243	3.1e-136	46.01	(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1	[more]

BLAST of Tan0022232 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899448.1	2.2e-272	86.12	(R)-mandelonitrile lyase-like [Benincasa hispida]	[more]
XP_023000184.1	2.0e-265	83.81	(R)-mandelonitrile lyase-like [Cucurbita maxima]	[more]
XP_022964434.1	2.6e-265	83.63	(R)-mandelonitrile lyase-like [Cucurbita moschata]	[more]
XP_023514980.1	5.9e-265	83.63	(R)-mandelonitrile lyase-like [Cucurbita pepo subsp. pepo]	[more]
KGN45431.1	2.6e-260	82.53	hypothetical protein Csa_016192 [Cucumis sativus]	[more]

BLAST of Tan0022232 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KJ63	9.8e-266	83.81	(R)-mandelonitrile lyase-like OS=Cucurbita maxima OX=3661 GN=LOC111494473 PE=3 S...	[more]
A0A6J1HN57	1.3e-265	83.63	(R)-mandelonitrile lyase-like OS=Cucurbita moschata OX=3662 GN=LOC111464460 PE=3...	[more]
A0A0A0KCB1	1.2e-260	82.53	GMC_OxRdtase_N domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G447...	[more]
A0A1S3CHZ4	1.4e-259	81.82	(R)-mandelonitrile lyase-like OS=Cucumis melo OX=3656 GN=LOC103500635 PE=3 SV=1	[more]
A0A5D3C0K1	3.0e-259	81.82	(R)-mandelonitrile lyase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]

BLAST of Tan0022232 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73050.1	4.1e-208	67.37	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G12570.1	5.9e-130	45.10	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51950.1	5.7e-125	44.19	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51950.2	2.4e-123	45.17	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G72970.1	3.1e-123	45.13	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.410.40		coord: 175..492 e-value: 2.2E-162 score: 543.4
None	No IPR available	PANTHER	PTHR45968	OSJNBA0019K04.7 PROTEIN	coord: 34..557
None	No IPR available	PANTHER	PTHR45968:SF2	(R)-MANDELONITRILE LYASE-LIKE	coord: 34..557
None	No IPR available	SUPERFAMILY	54373	FAD-linked reductases, C-terminal domain	coord: 325..499
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 53..542 e-value: 2.2E-162 score: 543.4
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 41..351
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PFAM	PF00732	GMC_oxred_N	coord: 53..329 e-value: 6.2E-31 score: 107.9
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PROSITE	PS00624	GMC_OXRED_2	coord: 288..302
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PROSITE	PS00623	GMC_OXRED_1	coord: 127..150
IPR012132	Glucose-methanol-choline oxidoreductase	PIRSF	PIRSF000137	Alcohol_oxidase	coord: 367..560 e-value: 3.1E-27 score: 93.5 coord: 38..364 e-value: 1.4E-58 score: 197.1
IPR007867	Glucose-methanol-choline oxidoreductase, C-terminal	PFAM	PF05199	GMC_oxred_C	coord: 398..543 e-value: 3.3E-27 score: 95.7

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0022232.1	Tan0022232.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0016614	oxidoreductase activity, acting on CH-OH group of donors