Tan0022147 (gene) Snake gourd v1

Overview
NameTan0022147
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionCoA ligase
LocationLG11: 13169307 .. 13172792 (+)
RNA-Seq ExpressionTan0022147
SyntenyTan0022147
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAAGCTTTTGAAATGTGATGCAAATTATGCTTCTCTTTCGCCCATCACTTTTCTCAAGAGAGCTTCTGCATTTTACTCTGATCGGACCTCCATTATCTATGAGGGTACTCGCTTCACATGGCGTCAAACTTATGAGCGCTGCTGTCGCCTCGCCTCCTCCCTTTGTCGTCTCAACATCGCCAAGAACGACGTCGTAAGACCTCTTTTTCGTTGCTCTCTTCTTCTTCCTCTTTCTTTCACTTTGATTTTCTTTAAGAGTAGATGTTAAAAATGAAACAAAGGGTAAAAATTTATGTCACATAATACTTAATAAGTATATAAAAAAAATCTGGTGATTTTTTTTAGTACATCAACAATTGGAGGTGAGAGATTTTTGTTGGTCATGAGTTATATTTAATATCAATTGAGTTAGGCTCTTGTTAACAAAATTTGAGGAATTAAGTCATCTAAAAATAATAATAATAATAATTTTGACTTCTTCTAATTTTTGAATATCCGTGTAAATTTATAGTTTATTTTAAAAAATTAAGTCTATAAAGATTATTTCTACTCGTGAATATCATTTAATGACTATTTTGTTTTTTTAGTTTTGATTTTTGAAAATTATATTTATTTTTTTTTTTACTATTTCTCTTTATCTTCTCTATCATGATTTCTATTTTTTCTTGAGTAATCATTTGAGTTTCTAAGCAAATTTTAAAAACAAAACACATGTTTTTGAAAACTATTTTTTTGAGTTTTCAAAACTAGTGGAATTTTTAGAAACTAAAGTATTAAATAAATAAGAAAACATGAAAATCTCTGAGTTGAAACAATTTTTTTAAACTAAAAACAAAAAAAATCAAACCGTTATGAAAGGAAGAAATATTATATATTTTTTTATTTTAGAATTTGATTAAGAATTCAAGTAACGGTGATTTACTCATGATTCTCGAAGGTGTCGGTGTTGGCGCCAAATGTTCCAGCATTGTACGAGATGCATTTCGCAGTGCCGATGGCAGGCGCTGTTTTGAACACAATCAACACCCGCCTCGATGTCAAGAACATCGCCATCATCCTCCGCCACGCCGAGTCCAAAATCCTCTTTGTCGACTACCAATACATACAACAAGCTAAAGATGCCCTCCGTTTGCTGATGGCGGAGTCCTCCCCGCTCCCACTCGTCGTCGTCATCGACGACATCGATTCTCCGACCGGGGCCCGGCTCGGGAACTTAGAATACGAGCAACTCATCCACGACGGTGATGTCGGGTTCGTCCCGATAGAGATCGACGACGAATGGGACTCGATAGCTCTGAATTACACTTCGGGCACGACGTCGGCGCCGAAGGGCGTGGTGTACAGCCACCGCGGCGCGTTCCTCAGCACGCTCAGCCTGGTGATGGGTTGGGAAATGGGCAATGCGCCTGTTTATCTGTGGACTCTGCCGATGTTCCACTGTAACGGATGGACCTTCACGTGGGGGATTGCAGCCCGTGGTGGGACCAACGTCTGTATGCGTAATACCACGGCGTTTGATATCTACCGGAATATTCATTTGCATCACGTGACTCACATGTGCTGTGCCCCCATTGTGTTCAGTATCATCCTCGAGCCCGACGGCGGCGAACGGCAGCGAGTTGGGTGGCCGGTGAACGTGCTGACTGGCGGAGCGCCGCCGCCGCCACCGCTTCTGGAAAAGATGGAGGCTCTTGGTTTTCATATAACTCATGCTTATGGGCTGACGGAGGCCACCGGACCGGCGCTGGTCTGCGAGTGGCAACAAAAGTGGAACGTTCTGCCGGTCGATGAACAAGCCAAGCTTAAAGCTAGACAAGGTACTTTTTTATTTAACAATTATTTAAATTCTCTTTTTACGTGGATATTTATGATTTACATATTTATTGAATATAATTAAATATAAAAATTAGATTCTGAAAAGAGTTAGATACAGGTCTTTTTTACGTTACTACAAGTTCGGTTTAATCTTAAAATGTTAAGAGAAAAAAATAAATTTTGATCCTAAATTTGTAAGTTATATCAATTTAGACTTTAAATAACTTTCAATTTTATTCATTGGACTTAAATAAATTTTAGCAATTTTTCCCTTTGGTTCAGCTAATATATTCACCAACTTATTTTGTAAAAATCTTATAAACTCACAAGATTCAGTAAAATAAAGTTTTTTTTTTTTCACAAAATAACTTTATAACCAGTGAAAAAAAAAACTTGCTCTAATGCCTTTTTTCTCTAAAATTTTAATTTTATATTGTGCATATAATTTATAATTCATTGCACATACCAGAAAAAAAAATTTAGCGATATTTGTAATGAGAGCTCATTAGAGAGTTAAAATTTTAAAATTTTTTAAAATTGATAGCAACATTTTTTTTAGCTTGAGTTTGAATTTCATAAAATTGAAAATTTATGATTAAAATGGCGTGATGAAAATTGTTTTTTCCTAAATTTTAAAATATGTAATATTTTTATTTACTTATTTTTTTTTTCAACAACACAATTGAAGGGGGATTGGAACAGGGACGAAGCTAAACTCATTTGGGTTAAAACTAAAAAAATGTTTTTTTTTTCTAAAAAAGAATTAATAAACAAATACCCAAAACAGAAAAATCAATCTCCACTAAAACTCACATGTCTGTTACCATCTATCACAATCAGGAATCAGCATTTTGACATTAGCAGATGTGGACGTCAAAAATTTGGACACAATGGAGGGTGTGCCGCACGACGGCCGGACCTCCGGGGAGATCGTCCTACGTGGCAGTAGCATCATGAAGGGCTACTTCAAGGATCCGGCAGCCACAGCGGCGGCCTTAAAAAATGGCTGGTTCCTCACCGGCGACGTCGGCGTCATTCACCCCGACGGCTATTTGGAGATCAAAGACAGATCCAAGGACGTTATCATCTCCGGCGGCGAGAACATCAGCAGCGTTGAGCTCGAGACAGTACTCTACCGCCACCCGCAGGTGGCGGAGGCGGCGGTGGTCGCAATGCCACATCCCCGCTGGGGGGAAAGCCCCTGTGCCTTCGTCGCCATCCGAGGCGGAGAGCAAGGCGGCGCCGGCAGGGTGAGCGAGGCGGAGATCATTGCGTTTTGCCGGAAGAGTATGGCACACTTTATGGTGCCGAAGAAGGTGGTATTCGTGGCGGAGCTTCCAAAGACGTCGACCGGAAAGATTCAGAAGTTCAAATTGAGGGAGCAGGCGAAATTATTCGTTGTCTCTGAAAAACGAGTCGGCGGCGGGAACAAGTCCGGCCGGCAAGACGGTGGCCGGCGGCAGGATTACGGTGAGCATGTTTTGGCTATGTCTCGGGCTATGTCTCGGCTTTGAGAGGGGTCCCATATGGGTTGCAATAAATAAATTGTACAAAATTAAAAATATATATGTACGTATTTTGTTATGATTTTGAGCCTGTTTTGTTTCTAAGACGTTGGGATATTGATCAAGAATGAGCAGTGGATAAAAATTGTGTAAGAAATTGA

mRNA sequence

ATGGAGAAGCTTTTGAAATGTGATGCAAATTATGCTTCTCTTTCGCCCATCACTTTTCTCAAGAGAGCTTCTGCATTTTACTCTGATCGGACCTCCATTATCTATGAGGGTACTCGCTTCACATGGCGTCAAACTTATGAGCGCTGCTGTCGCCTCGCCTCCTCCCTTTGTCGTCTCAACATCGCCAAGAACGACGTCGTGTCGGTGTTGGCGCCAAATGTTCCAGCATTGTACGAGATGCATTTCGCAGTGCCGATGGCAGGCGCTGTTTTGAACACAATCAACACCCGCCTCGATGTCAAGAACATCGCCATCATCCTCCGCCACGCCGAGTCCAAAATCCTCTTTGTCGACTACCAATACATACAACAAGCTAAAGATGCCCTCCGTTTGCTGATGGCGGAGTCCTCCCCGCTCCCACTCGTCGTCGTCATCGACGACATCGATTCTCCGACCGGGGCCCGGCTCGGGAACTTAGAATACGAGCAACTCATCCACGACGGTGATGTCGGGTTCGTCCCGATAGAGATCGACGACGAATGGGACTCGATAGCTCTGAATTACACTTCGGGCACGACGTCGGCGCCGAAGGGCGTGGTGTACAGCCACCGCGGCGCGTTCCTCAGCACGCTCAGCCTGGTGATGGGTTGGGAAATGGGCAATGCGCCTGTTTATCTGTGGACTCTGCCGATGTTCCACTGTAACGGATGGACCTTCACGTGGGGGATTGCAGCCCGTGGTGGGACCAACGTCTGTATGCGTAATACCACGGCGTTTGATATCTACCGGAATATTCATTTGCATCACGTGACTCACATGTGCTGTGCCCCCATTGTGTTCAGTATCATCCTCGAGCCCGACGGCGGCGAACGGCAGCGAGTTGGGTGGCCGGTGAACGTGCTGACTGGCGGAGCGCCGCCGCCGCCACCGCTTCTGGAAAAGATGGAGGCTCTTGGTTTTCATATAACTCATGCTTATGGGCTGACGGAGGCCACCGGACCGGCGCTGGTCTGCGAGTGGCAACAAAAGTGGAACGTTCTGCCGGTCGATGAACAAGCCAAGCTTAAAGCTAGACAAGGAATCAGCATTTTGACATTAGCAGATGTGGACGTCAAAAATTTGGACACAATGGAGGGTGTGCCGCACGACGGCCGGACCTCCGGGGAGATCGTCCTACGTGGCAGTAGCATCATGAAGGGCTACTTCAAGGATCCGGCAGCCACAGCGGCGGCCTTAAAAAATGGCTGGTTCCTCACCGGCGACGTCGGCGTCATTCACCCCGACGGCTATTTGGAGATCAAAGACAGATCCAAGGACGTTATCATCTCCGGCGGCGAGAACATCAGCAGCGTTGAGCTCGAGACAGTACTCTACCGCCACCCGCAGGTGGCGGAGGCGGCGGTGGTCGCAATGCCACATCCCCGCTGGGGGGAAAGCCCCTGTGCCTTCGTCGCCATCCGAGGCGGAGAGCAAGGCGGCGCCGGCAGGGTGAGCGAGGCGGAGATCATTGCGTTTTGCCGGAAGAGTATGGCACACTTTATGGTGCCGAAGAAGGTGGTATTCGTGGCGGAGCTTCCAAAGACGTCGACCGGAAAGATTCAGAAGTTCAAATTGAGGGAGCAGGCGAAATTATTCGTTGTCTCTGAAAAACGAGTCGGCGGCGGGAACAAGTCCGGCCGGCAAGACGGTGGCCGGCGGCAGGATTACGGTGAGCATGTTTTGGCTATGTCTCGGGCTATGTCTCGGCTTTGAGAGGGGTCCCATATGGGTTGCAATAAATAAATTGTACAAAATTAAAAATATATATGTACGTATTTTGTTATGATTTTGAGCCTGTTTTGTTTCTAAGACGTTGGGATATTGATCAAGAATGAGCAGTGGATAAAAATTGTGTAAGAAATTGA

Coding sequence (CDS)

ATGGAGAAGCTTTTGAAATGTGATGCAAATTATGCTTCTCTTTCGCCCATCACTTTTCTCAAGAGAGCTTCTGCATTTTACTCTGATCGGACCTCCATTATCTATGAGGGTACTCGCTTCACATGGCGTCAAACTTATGAGCGCTGCTGTCGCCTCGCCTCCTCCCTTTGTCGTCTCAACATCGCCAAGAACGACGTCGTGTCGGTGTTGGCGCCAAATGTTCCAGCATTGTACGAGATGCATTTCGCAGTGCCGATGGCAGGCGCTGTTTTGAACACAATCAACACCCGCCTCGATGTCAAGAACATCGCCATCATCCTCCGCCACGCCGAGTCCAAAATCCTCTTTGTCGACTACCAATACATACAACAAGCTAAAGATGCCCTCCGTTTGCTGATGGCGGAGTCCTCCCCGCTCCCACTCGTCGTCGTCATCGACGACATCGATTCTCCGACCGGGGCCCGGCTCGGGAACTTAGAATACGAGCAACTCATCCACGACGGTGATGTCGGGTTCGTCCCGATAGAGATCGACGACGAATGGGACTCGATAGCTCTGAATTACACTTCGGGCACGACGTCGGCGCCGAAGGGCGTGGTGTACAGCCACCGCGGCGCGTTCCTCAGCACGCTCAGCCTGGTGATGGGTTGGGAAATGGGCAATGCGCCTGTTTATCTGTGGACTCTGCCGATGTTCCACTGTAACGGATGGACCTTCACGTGGGGGATTGCAGCCCGTGGTGGGACCAACGTCTGTATGCGTAATACCACGGCGTTTGATATCTACCGGAATATTCATTTGCATCACGTGACTCACATGTGCTGTGCCCCCATTGTGTTCAGTATCATCCTCGAGCCCGACGGCGGCGAACGGCAGCGAGTTGGGTGGCCGGTGAACGTGCTGACTGGCGGAGCGCCGCCGCCGCCACCGCTTCTGGAAAAGATGGAGGCTCTTGGTTTTCATATAACTCATGCTTATGGGCTGACGGAGGCCACCGGACCGGCGCTGGTCTGCGAGTGGCAACAAAAGTGGAACGTTCTGCCGGTCGATGAACAAGCCAAGCTTAAAGCTAGACAAGGAATCAGCATTTTGACATTAGCAGATGTGGACGTCAAAAATTTGGACACAATGGAGGGTGTGCCGCACGACGGCCGGACCTCCGGGGAGATCGTCCTACGTGGCAGTAGCATCATGAAGGGCTACTTCAAGGATCCGGCAGCCACAGCGGCGGCCTTAAAAAATGGCTGGTTCCTCACCGGCGACGTCGGCGTCATTCACCCCGACGGCTATTTGGAGATCAAAGACAGATCCAAGGACGTTATCATCTCCGGCGGCGAGAACATCAGCAGCGTTGAGCTCGAGACAGTACTCTACCGCCACCCGCAGGTGGCGGAGGCGGCGGTGGTCGCAATGCCACATCCCCGCTGGGGGGAAAGCCCCTGTGCCTTCGTCGCCATCCGAGGCGGAGAGCAAGGCGGCGCCGGCAGGGTGAGCGAGGCGGAGATCATTGCGTTTTGCCGGAAGAGTATGGCACACTTTATGGTGCCGAAGAAGGTGGTATTCGTGGCGGAGCTTCCAAAGACGTCGACCGGAAAGATTCAGAAGTTCAAATTGAGGGAGCAGGCGAAATTATTCGTTGTCTCTGAAAAACGAGTCGGCGGCGGGAACAAGTCCGGCCGGCAAGACGGTGGCCGGCGGCAGGATTACGGTGAGCATGTTTTGGCTATGTCTCGGGCTATGTCTCGGCTTTGA

Protein sequence

MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLNIAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQYIQQAKDALRLLMAESSPLPLVVVIDDIDSPTGARLGNLEYEQLIHDGDVGFVPIEIDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVGWPVNVLTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKLKARQGISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESPCAFVAIRGGEQGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTSTGKIQKFKLREQAKLFVVSEKRVGGGNKSGRQDGGRRQDYGEHVLAMSRAMSRL
Homology
BLAST of Tan0022147 vs. ExPASy Swiss-Prot
Match: I3PB36 (Trans-cinnamate:CoA ligase, peroxisomal OS=Petunia hybrida OX=4102 GN=CNL PE=1 SV=1)

HSP 1 Score: 747.7 bits (1929), Expect = 1.0e-214
Identity = 365/556 (65.65%), Postives = 429/556 (77.16%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M++L KC ANY  L+P+TFL RA   Y++RTSIIY G RFTW QTY+RCCRLASSL  LN
Sbjct: 1   MDELPKCGANYVPLTPLTFLTRAFKSYANRTSIIYAGARFTWEQTYKRCCRLASSLQSLN 60

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           I KNDVVSVLAPNVPA YEMHFAVPMAGAVLNTINTRLD  NIAIIL+H+E+K+LFVDY+
Sbjct: 61  IVKNDVVSVLAPNVPATYEMHFAVPMAGAVLNTINTRLDPMNIAIILKHSEAKLLFVDYE 120

Query: 121 YIQQAKDALRLLM---------AESSPLPLVVVIDDIDSPTG-ARLGNLEYEQLIHDGDV 180
           Y+++A+ AL LLM         ++   +P V++IDD+ SPT   +   LEYEQL+H G+ 
Sbjct: 121 YLEKARKALELLMSTNFITAQNSKKISMPQVILIDDLYSPTRIQQQDQLEYEQLVHQGNP 180

Query: 181 GFVPIEI-DDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTL 240
            + P  I DDEWD I LNYTSGTTS PKGVVYSHRGAFLSTL+ +MGWEMG  PVYLW+L
Sbjct: 181 EYAPENIVDDEWDPIVLNYTSGTTSEPKGVVYSHRGAFLSTLNTIMGWEMGTEPVYLWSL 240

Query: 241 PMFHCNGWTFTWGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGG 300
           PMFH NGWT TWGIAARGGTNVC+RNTTA +IY NI LH VTHMCCAP VF+I+LE    
Sbjct: 241 PMFHINGWTLTWGIAARGGTNVCIRNTTAQEIYSNITLHKVTHMCCAPTVFNILLEAKPH 300

Query: 301 ERQRVGWPVNVLTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPV 360
           ER+ +  PV V+ GGAPPP  L+ K+E LGFH+ H YG+TEA G  LVCEWQ +WN L  
Sbjct: 301 ERREITTPVQVMVGGAPPPTTLIGKIEELGFHVVHCYGITEAGGTTLVCEWQSEWNKLSR 360

Query: 361 DEQAKLKARQGISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATA 420
           ++QA LKARQGIS+L L DVDVKN  TM+ VPH+G+T GEI LRGSSIMKGYFK+  A +
Sbjct: 361 EDQANLKARQGISVLALEDVDVKNSKTMQSVPHNGKTMGEICLRGSSIMKGYFKNDKANS 420

Query: 421 AALKNGWFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVV 480
              KNGWFLTGDV VIH DGYLEIKDR KD+IISGGENISS+E+E  + +HP V EAAVV
Sbjct: 421 QVFKNGWFLTGDVAVIHQDGYLEIKDRCKDIIISGGENISSIEVENAILKHPSVIEAAVV 480

Query: 481 AMPHPRWGESPCAFVAIRGGEQGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTS 540
           AMPHPRWGE+PCAFV      +     + EA+II  C+K +  FMVPK V F+ ELPKT 
Sbjct: 481 AMPHPRWGETPCAFVIKTKNPE-----IKEADIIVHCKKELPGFMVPKHVQFLEELPKTG 540

Query: 541 TGKIQKFKLREQAKLF 546
           TGK++K +LRE AK F
Sbjct: 541 TGKVKKLQLREMAKSF 551

BLAST of Tan0022147 vs. ExPASy Swiss-Prot
Match: Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 707.6 bits (1825), Expect = 1.2e-202
Identity = 346/558 (62.01%), Postives = 421/558 (75.45%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M+ L+ C+AN   L+PITFLKRAS  Y +RTSIIY  TRFTW QTY+RCCRLA+SL  LN
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           I +NDVVS+LAPNVPA+YEMHF+VPM GAVLN INTRLD K IAIILRHAE KILFVDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 YIQQAKDALRLLMA-ESSPLPLVVVIDDIDSPTGARLGNLEYEQLIHDGD----VGFVPI 180
           +    ++ LRL+   +S P P +++I++IDS T      L+YE LI  G+          
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASMF 180

Query: 181 EIDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            + +E D I+LNYTSGTT+ PKGVV SH+GA+LS LS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVG 300
           GWT TW +AARGGTNVC+R+ TA +IY+NI LH VTHM C P VF  +LE    ++    
Sbjct: 241 GWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKS 300

Query: 301 WPVNVLTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKL 360
            PV VLTGG+ PP  L++K+E LGFH+ H YGLTEATGP L CEWQ +WN LP  +Q +L
Sbjct: 301 SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIEL 360

Query: 361 KARQGISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNG 420
           + RQG+  LTLADVDVKN  T+E VP DG+T GEIV++GSS+MKGY K+P AT+ A K+G
Sbjct: 361 QQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  TGD+GVIHPDGY+EIKDRSKD+IISGGENISS+E+E VLY + +V EAAVVAMPHP 
Sbjct: 421 WLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMPHPL 480

Query: 481 WGESPCAFVAIRGGEQGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTSTGKIQK 540
           WGE+PCAFV ++ GE+G     SE ++I +CR++M HFM PKKVVF  ELPK S GKI K
Sbjct: 481 WGETPCAFVVLKKGEEGLV--TSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILK 540

Query: 541 FKLREQAKLFVVSEKRVG 554
            KLR+ AK  VV E   G
Sbjct: 541 SKLRDIAKALVVREDDAG 556

BLAST of Tan0022147 vs. ExPASy Swiss-Prot
Match: Q9SS00 (Probable acyl-activating enzyme 12, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE12 PE=2 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 1.0e-198
Identity = 341/560 (60.89%), Postives = 422/560 (75.36%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M+ L  C+AN   L+PITFLKRAS  Y +RTSIIY  TRFTW QTY+RCCRLA+SL  LN
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           I KNDVVSV+APN PA+YEMHFAVPMAGAVLN INTRLD  +IA ILRHA+ KILF+   
Sbjct: 61  IGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRS 120

Query: 121 YIQQAKDALRLLMAESSPLPL-VVVIDDIDSPTGARLGNLEYEQLIHDGDVGFVPIE--- 180
           +   A++ L+LL +E S L L V+ I +ID P        +YE LI  G+   + +    
Sbjct: 121 FEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQRGEPTPLLLARMF 180

Query: 181 -IDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            I DE D I+LNYTSGTT+ PKGVV SHRGA+LSTLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 CIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVG 300
           GWTFTWG AARGGT+VCMR+ TA +IY+NI +H+VTHMCC P VF+I+L+ +  +     
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSHRS 300

Query: 301 WPVNVLTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKL 360
            PV+VLTGG+PPP  L++K++ LGF + HAYGLTEATGP L CEWQ +WN LP ++Q +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNG 420
           KARQG+SIL L +VDV+N +T E VP DG+T GEIV++GSSIMKGY K+P AT  A K+G
Sbjct: 361 KARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  +GDVGVIHPDG++EIKDRSKD+IISGGENISSVE+E ++Y++P+V E AVVAMPHP 
Sbjct: 421 WLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFVAIRGGEQGGAGR-----VSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTST 540
           WGE+PCAFV +  GE     R       E ++I +CR+++ HFM P+KVVF+ ELPK   
Sbjct: 481 WGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPKNGN 540

Query: 541 GKIQKFKLREQAKLFVVSEK 551
           GKI K KLR+ AK  V  ++
Sbjct: 541 GKILKPKLRDIAKGLVAEDE 560

BLAST of Tan0022147 vs. ExPASy Swiss-Prot
Match: Q9SS01 (Benzoate--CoA ligase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE20 PE=1 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 3.9e-198
Identity = 344/561 (61.32%), Postives = 422/561 (75.22%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M+ L  C+AN   L+P+TFLKRAS  Y +RTSIIY  TRFTW QTY+RCCRLA+SL  LN
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           I+KNDVVSV+APN PALYEMHFAVPMAGAVLN INTRLD  +IA ILRHA+ KILF+D  
Sbjct: 61  ISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRS 120

Query: 121 YIQQAKDALRLLMAESSPLPL-VVVIDDIDSPTGARLGNLEYEQLIHDGD----VGFVPI 180
           +   A+++L LL +E S L L V+ I + D P  A    L+YE LI  G+    +     
Sbjct: 121 FEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLIQRGEPTPSMVARMF 180

Query: 181 EIDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            I DE D I+LNYTSGTT+ PKGVV SHRGA+L TLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVG 300
           GWTFTWG AARGGT+VCMR+ TA +IY+NI +H+VTHMCC P VF+I+L+ +  +     
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRS 300

Query: 301 WPVNVLTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKL 360
            PV+VLTGG+PPP  L++K++ LGF + HAYG TEATGP L CEWQ +WN LP ++Q +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNG 420
           KARQGISIL LADVDVKN +T +  P DG+T GEI+++GSSIMKGY K+P AT  A K+G
Sbjct: 361 KARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKATFEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  TGDVGVIHPDG++EIKDRSKD+IISGGENISSVE+E VLY++P+V E AVVAMPHP 
Sbjct: 421 WLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFVAIRGGE------QGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTS 540
           WGE+PCAFV +   E      +    +  E  +I +CR+++ HFM P+KVVF+ ELPK  
Sbjct: 481 WGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKNG 540

Query: 541 TGKIQKFKLREQAKLFVVSEK 551
            GKI K KLR+ AK  VV ++
Sbjct: 541 NGKILKPKLRDIAKGLVVEDE 561

BLAST of Tan0022147 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 2.8e-164
Identity = 280/555 (50.45%), Postives = 391/555 (70.45%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           ME  +K  ANY  L+PI+FL R++  Y+DR SI+Y   ++TWRQT +RC R+AS+L +L 
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           I+  DVVSVLAPNVPA+ E+HF VPMAGA+L T+N R D   +A++LRH+ +K++F D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 YIQQAKDALRLLMAESSPLPLVVVIDDIDSPTGARLGN----LEYEQLIHDGDVGFVPIE 180
           ++Q A+ A  +L  +   +P++V+I +  + + +R       +EYE ++  G   F  I 
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 181 IDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNG 240
             DE D+I++NYTSGTTS+PKGVVYSHRGA+L++L+ V+  EM ++P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 241 WTFTWGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVGW 300
           W   WG+ A GGTN+C+RN TA  I+ NI  H VTHM  AP + ++I+     E++ +  
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 301 PVNVLTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKLK 360
            V+ +TG APPP  ++ KME LGF + H+YGLTE  GP  +C W+ +W+ LP +EQAK+K
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

Query: 361 ARQGISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNGW 420
           ARQG++ L L ++ VK+  TM  +P DG T GE+V RG+++M GY K+P AT  A K GW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422

Query: 421 FLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRW 480
           F +GD+GV HPDGY+E+KDRSKD+IISGGENISS+E+E+ L+ HP V EAAVVA P   W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482

Query: 481 GESPCAFVAIRGGEQGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTSTGKIQKF 540
           GE+ CAFV ++ G      + S  E+I++CR  + H+M P+ +VF  +LPKTSTGK+QKF
Sbjct: 483 GETACAFVKLKDG-----SKASAEELISYCRDRLPHYMAPRSIVF-EDLPKTSTGKVQKF 542

Query: 541 KLREQAKLFVVSEKR 552
            LR +AK  V   K+
Sbjct: 543 VLRTKAKALVSLSKK 551

BLAST of Tan0022147 vs. NCBI nr
Match: XP_022923630.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita moschata])

HSP 1 Score: 1047.7 bits (2708), Expect = 3.6e-302
Identity = 505/578 (87.37%), Postives = 538/578 (93.08%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M  L KCDANYASL+PITFLKRASAFY+DRTSIIYEGTRFTWRQTY+RCCRLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           +AKN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRH+E+KILFVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKDALRLLMAESSPLPLVVVIDDIDSPTGARLGNLEYEQLIHDGDVGFVPIEIDDE 180
           +IQ+AKDALR LMA S PLP VVVIDDIDSPTGAR G LEYEQL+HDGDVGF P+E+DDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVGWPVNV 300
           WGIAARGGTNVCMRNTTAFDIYRNI+LHHVTHMCCAPIVF IILE +GGER+R GW VNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKLKARQG 360
           LTGGAPPP  LLEKMEALGFHITHAYGLTEATGPALVCEWQ+KWN+LP DEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNL+TM+ VPHDGRT+GEIVLRGSSIMKGYFKDPAATA A +NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAIRGGEQGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTSTGKIQKFKLRE 540
           CAFV +        G+VSEAE+I FCRK+M+H+M PKKVVFVAELPKT TGKIQKFKLRE
Sbjct: 481 CAFVVV----HSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLRE 540

Query: 541 QAKLFVVSEKRVGGGNKSGRQDGGRRQDYGEHVLAMSR 579
           QAKL V SEKRV GGNKSGR DGGR+ DYG+HV+AMSR
Sbjct: 541 QAKLLVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSR 574

BLAST of Tan0022147 vs. NCBI nr
Match: XP_023513711.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1044.3 bits (2699), Expect = 4.0e-301
Identity = 503/578 (87.02%), Postives = 537/578 (92.91%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M  L KCDANYASL+PITFLKRASAFY+DRTSIIYEGTRFTWRQTYERCCRLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYERCCRLASSLSRLN 60

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           + KN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRH+E+KILFVDYQ
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKDALRLLMAESSPLPLVVVIDDIDSPTGARLGNLEYEQLIHDGDVGFVPIEIDDE 180
           +IQ+AKDALR LMA S PLP VVVIDDIDSPTGAR G LEYEQL+HDGDVGF P+E+DDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLV+GWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVVGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVGWPVNV 300
           WGIAARGGTNVCMRNTTAFDIYRNI+LHHVTHMCCAPIVF IILE +GGER+R GW VNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKLKARQG 360
           LTGGAPPP  LLEKMEALGFHITHAYGLTEATGPALVCEWQ+KWN+LP DEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNL+TM+ VPHDGRT+GEIVLRGSSIMKGYFKDPAATA A +NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAIRGGEQGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTSTGKIQKFKLRE 540
           CAFV +        G+VSEAE+I FCRK+M+H+M PKKVVFVAELPKT TGKIQKFKLRE
Sbjct: 481 CAFVVV----HSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLRE 540

Query: 541 QAKLFVVSEKRVGGGNKSGRQDGGRRQDYGEHVLAMSR 579
           QAKL V SEKRV GGNKSGR DGGR+ +YG+HV+AMSR
Sbjct: 541 QAKLLVGSEKRVSGGNKSGRHDGGRQPEYGQHVMAMSR 574

BLAST of Tan0022147 vs. NCBI nr
Match: KAG6602004.1 (Benzoate--CoA ligase, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1043.9 bits (2698), Expect = 5.2e-301
Identity = 504/578 (87.20%), Postives = 537/578 (92.91%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M  L KCDANYASL+PITFLKRASAFY+DRTSIIYEGTRFTWRQTY+RCCRLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           +AKN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRH+E+KILFVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKDALRLLMAESSPLPLVVVIDDIDSPTGARLGNLEYEQLIHDGDVGFVPIEIDDE 180
           +IQ+AKDALR LMA S PLP VVVIDDIDSPTGAR G LEYEQL+HDGDVGF P+E+DDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVGWPVNV 300
           WGIAARGGTNVCMRNTTAFDIYRNI+LHHVTHMCCAPIVF IILE +GGER+R GW VNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKLKARQG 360
           LTGGAPPP  LLEKMEALGFHITHAYGLTEATGPALVCEWQ+KWN+LP DEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNL+TM+ VPHDGRT+GEIVLRGSSIMKGYFKD AATA A +NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDLAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAIRGGEQGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTSTGKIQKFKLRE 540
           CAFV +        G+VSEAE+I FCRK+M+H+M PKKVVFVAELPKT TGKIQKFKLRE
Sbjct: 481 CAFVVV----HSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLRE 540

Query: 541 QAKLFVVSEKRVGGGNKSGRQDGGRRQDYGEHVLAMSR 579
           QAKL V SEKRV GGNKSGR DGGR+ DYG+HV+AMSR
Sbjct: 541 QAKLLVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSR 574

BLAST of Tan0022147 vs. NCBI nr
Match: XP_022990938.1 (butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita maxima])

HSP 1 Score: 1038.9 bits (2685), Expect = 1.7e-299
Identity = 500/578 (86.51%), Postives = 535/578 (92.56%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M  L KCDAN+ASL+PITFLKRASAFY+DRTSIIYEGT FTWRQTYERCC LASSL RLN
Sbjct: 1   MNSLSKCDANFASLTPITFLKRASAFYADRTSIIYEGTSFTWRQTYERCCHLASSLSRLN 60

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           + KN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRH+E+KILFVDYQ
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKDALRLLMAESSPLPLVVVIDDIDSPTGARLGNLEYEQLIHDGDVGFVPIEIDDE 180
           +IQ+AKDALR LMA S PLP VVVIDDIDSPTGAR G LEYEQL+HDGDVGF P+E+DDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVGWPVNV 300
           WGIAARGGTNVC+RNTTAFDIYRNI+LHHVTHMCCAPIVF IILE +GGER+R GW VNV
Sbjct: 241 WGIAARGGTNVCIRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKLKARQG 360
           LTGGAPPP  LLEKMEALGFHITHAYGLTEATGPALVCEWQ+KWN+LP DEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNL+TM+ VPHDGRT+GEIVLRGSSIMKGYFKDPAATA A +NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAIRGGEQGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTSTGKIQKFKLRE 540
           CAFV +       +G+VSEAE+I FCRK+M+H+M PKKV+FVAELPKT TGKIQKFKLRE
Sbjct: 481 CAFVVV----HSDSGKVSEAEMIEFCRKNMSHYMAPKKVLFVAELPKTLTGKIQKFKLRE 540

Query: 541 QAKLFVVSEKRVGGGNKSGRQDGGRRQDYGEHVLAMSR 579
           QAKL V SEKRV GGNKSGR DGGR  +YGEHV+AMSR
Sbjct: 541 QAKLLVASEKRVSGGNKSGRHDGGRLPEYGEHVMAMSR 574

BLAST of Tan0022147 vs. NCBI nr
Match: XP_011655134.1 (butyrate--CoA ligase AAE11, peroxisomal [Cucumis sativus] >KGN51049.2 hypothetical protein Csa_023114 [Cucumis sativus])

HSP 1 Score: 1028.9 bits (2659), Expect = 1.7e-296
Identity = 493/578 (85.29%), Postives = 542/578 (93.77%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           ME LLKCDANY++L+PITFLKRASAFY+DRTSIIYEGTRFTW+QTYERCCRLASSLCRL 
Sbjct: 1   MEMLLKCDANYSALTPITFLKRASAFYADRTSIIYEGTRFTWKQTYERCCRLASSLCRLT 60

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           ++KNDVVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLD+KNIA+ILRH+E+KI FVDYQ
Sbjct: 61  VSKNDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDIKNIALILRHSEAKIFFVDYQ 120

Query: 121 YIQQAKDALRLLMAESSPLPLVVVIDDIDSPTGARLGNLEYEQLIHDGDVGFVPIEIDDE 180
           YIQ+AKDALRLL+AESSP+PLVVVIDDID+PTGARLGNLEYEQLI+DGD  F P+E+DDE
Sbjct: 121 YIQEAKDALRLLVAESSPIPLVVVIDDIDTPTGARLGNLEYEQLIYDGDANFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVGWPVNV 300
           WGIAARGGTN+CMRNTTA DI+RNI+LH VTHMCCAPIVF+IILE D  +R+RV WPVNV
Sbjct: 241 WGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFTIILEADNRDRRRVAWPVNV 300

Query: 301 LTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKLKARQG 360
           LTGGAPPP PLLEKMEALGFHITHAYGLTEATGPALVCEWQ+KWNVLP D+QA LKARQG
Sbjct: 301 LTGGAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQG 360

Query: 361 ISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNL TME VPHDGRT+GEIVLRGSS+MKGYFKDPAATAAALKNGWFLTG
Sbjct: 361 ISILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPAATAAALKNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           D+GVI+PDGY+EIKDRSKDVIISGGENISSVE+ETVLYRHPQV+EAAVVAMPHPRWGESP
Sbjct: 421 DIGVIYPDGYVEIKDRSKDVIISGGENISSVEVETVLYRHPQVSEAAVVAMPHPRWGESP 480

Query: 481 CAFVAIRGGEQGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTSTGKIQKFKLRE 540
           CAFV ++ G  GG+GRVSEAE++AFCRK+++H+MVPKKVVFVAELPK + GK+QKF LRE
Sbjct: 481 CAFVVLQDG--GGSGRVSEAEMVAFCRKNLSHYMVPKKVVFVAELPK-ANGKVQKFMLRE 540

Query: 541 QAKLFVVSEKRVGGGNKSGRQDGGRRQDYGEHVLAMSR 579
           QAK   VSEKR+   +  G + G RR DYGEHV+AMSR
Sbjct: 541 QAKYLEVSEKRI--VSNMGNKSGNRRSDYGEHVIAMSR 573

BLAST of Tan0022147 vs. ExPASy TrEMBL
Match: A0A6J1ECD9 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LOC111431279 PE=4 SV=1)

HSP 1 Score: 1047.7 bits (2708), Expect = 1.8e-302
Identity = 505/578 (87.37%), Postives = 538/578 (93.08%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M  L KCDANYASL+PITFLKRASAFY+DRTSIIYEGTRFTWRQTY+RCCRLASSL RLN
Sbjct: 1   MNSLSKCDANYASLTPITFLKRASAFYADRTSIIYEGTRFTWRQTYQRCCRLASSLSRLN 60

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           +AKN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRH+E+KILFVDYQ
Sbjct: 61  VAKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVNNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKDALRLLMAESSPLPLVVVIDDIDSPTGARLGNLEYEQLIHDGDVGFVPIEIDDE 180
           +IQ+AKDALR LMA S PLP VVVIDDIDSPTGAR G LEYEQL+HDGDVGF P+E+DDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVGWPVNV 300
           WGIAARGGTNVCMRNTTAFDIYRNI+LHHVTHMCCAPIVF IILE +GGER+R GW VNV
Sbjct: 241 WGIAARGGTNVCMRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKLKARQG 360
           LTGGAPPP  LLEKMEALGFHITHAYGLTEATGPALVCEWQ+KWN+LP DEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNL+TM+ VPHDGRT+GEIVLRGSSIMKGYFKDPAATA A +NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAIRGGEQGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTSTGKIQKFKLRE 540
           CAFV +        G+VSEAE+I FCRK+M+H+M PKKVVFVAELPKT TGKIQKFKLRE
Sbjct: 481 CAFVVV----HSDGGKVSEAEMIEFCRKNMSHYMAPKKVVFVAELPKTLTGKIQKFKLRE 540

Query: 541 QAKLFVVSEKRVGGGNKSGRQDGGRRQDYGEHVLAMSR 579
           QAKL V SEKRV GGNKSGR DGGR+ DYG+HV+AMSR
Sbjct: 541 QAKLLVGSEKRVSGGNKSGRHDGGRQPDYGQHVMAMSR 574

BLAST of Tan0022147 vs. ExPASy TrEMBL
Match: A0A6J1JUR4 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC111487678 PE=4 SV=1)

HSP 1 Score: 1038.9 bits (2685), Expect = 8.2e-300
Identity = 500/578 (86.51%), Postives = 535/578 (92.56%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M  L KCDAN+ASL+PITFLKRASAFY+DRTSIIYEGT FTWRQTYERCC LASSL RLN
Sbjct: 1   MNSLSKCDANFASLTPITFLKRASAFYADRTSIIYEGTSFTWRQTYERCCHLASSLSRLN 60

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           + KN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLDV NIA+ILRH+E+KILFVDYQ
Sbjct: 61  VVKNTVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVSNIALILRHSEAKILFVDYQ 120

Query: 121 YIQQAKDALRLLMAESSPLPLVVVIDDIDSPTGARLGNLEYEQLIHDGDVGFVPIEIDDE 180
           +IQ+AKDALR LMA S PLP VVVIDDIDSPTGAR G LEYEQL+HDGDVGF P+E+DDE
Sbjct: 121 FIQEAKDALRQLMAASLPLPFVVVIDDIDSPTGARFGELEYEQLVHDGDVGFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGA+LSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAYLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVGWPVNV 300
           WGIAARGGTNVC+RNTTAFDIYRNI+LHHVTHMCCAPIVF IILE +GGER+R GW VNV
Sbjct: 241 WGIAARGGTNVCIRNTTAFDIYRNINLHHVTHMCCAPIVFRIILEANGGERRRFGWLVNV 300

Query: 301 LTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKLKARQG 360
           LTGGAPPP  LLEKMEALGFHITHAYGLTEATGPALVCEWQ+KWN+LP DEQAKLKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNILPADEQAKLKARQG 360

Query: 361 ISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNL+TM+ VPHDGRT+GEIVLRGSSIMKGYFKDPAATA A +NGWFLTG
Sbjct: 361 ISILTLADVDVKNLETMDSVPHDGRTTGEIVLRGSSIMKGYFKDPAATAKAFQNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           DVGVIHPDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAIRGGEQGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTSTGKIQKFKLRE 540
           CAFV +       +G+VSEAE+I FCRK+M+H+M PKKV+FVAELPKT TGKIQKFKLRE
Sbjct: 481 CAFVVV----HSDSGKVSEAEMIEFCRKNMSHYMAPKKVLFVAELPKTLTGKIQKFKLRE 540

Query: 541 QAKLFVVSEKRVGGGNKSGRQDGGRRQDYGEHVLAMSR 579
           QAKL V SEKRV GGNKSGR DGGR  +YGEHV+AMSR
Sbjct: 541 QAKLLVASEKRVSGGNKSGRHDGGRLPEYGEHVMAMSR 574

BLAST of Tan0022147 vs. ExPASy TrEMBL
Match: A0A0A0KT40 (Acyl:coa ligase acetate-coa synthetase-like protein OS=Cucumis sativus OX=3659 GN=Csa_5G319910 PE=4 SV=1)

HSP 1 Score: 1025.4 bits (2650), Expect = 9.3e-296
Identity = 491/575 (85.39%), Postives = 540/575 (93.91%), Query Frame = 0

Query: 4   LLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLNIAK 63
           LLKCDANY++L+PITFLKRASAFY+DRTSIIYEGTRFTW+QTYERCCRLASSLCRL ++K
Sbjct: 2   LLKCDANYSALTPITFLKRASAFYADRTSIIYEGTRFTWKQTYERCCRLASSLCRLTVSK 61

Query: 64  NDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQYIQ 123
           NDVVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLD+KNIA+ILRH+E+KI FVDYQYIQ
Sbjct: 62  NDVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDIKNIALILRHSEAKIFFVDYQYIQ 121

Query: 124 QAKDALRLLMAESSPLPLVVVIDDIDSPTGARLGNLEYEQLIHDGDVGFVPIEIDDEWDS 183
           +AKDALRLL+AESSP+PLVVVIDDID+PTGARLGNLEYEQLI+DGD  F P+E+DDEWDS
Sbjct: 122 EAKDALRLLVAESSPIPLVVVIDDIDTPTGARLGNLEYEQLIYDGDANFTPVEVDDEWDS 181

Query: 184 IALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWGI 243
           IALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWGI
Sbjct: 182 IALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFTWGI 241

Query: 244 AARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVGWPVNVLTG 303
           AARGGTN+CMRNTTA DI+RNI+LH VTHMCCAPIVF+IILE D  +R+RV WPVNVLTG
Sbjct: 242 AARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFTIILEADNRDRRRVAWPVNVLTG 301

Query: 304 GAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKLKARQGISI 363
           GAPPP PLLEKMEALGFHITHAYGLTEATGPALVCEWQ+KWNVLP D+QA LKARQGISI
Sbjct: 302 GAPPPAPLLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQGISI 361

Query: 364 LTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTGDVG 423
           LTLADVDVKNL TME VPHDGRT+GEIVLRGSS+MKGYFKDPAATAAALKNGWFLTGD+G
Sbjct: 362 LTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPAATAAALKNGWFLTGDIG 421

Query: 424 VIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESPCAF 483
           VI+PDGY+EIKDRSKDVIISGGENISSVE+ETVLYRHPQV+EAAVVAMPHPRWGESPCAF
Sbjct: 422 VIYPDGYVEIKDRSKDVIISGGENISSVEVETVLYRHPQVSEAAVVAMPHPRWGESPCAF 481

Query: 484 VAIRGGEQGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTSTGKIQKFKLREQAK 543
           V ++ G  GG+GRVSEAE++AFCRK+++H+MVPKKVVFVAELPK + GK+QKF LREQAK
Sbjct: 482 VVLQDG--GGSGRVSEAEMVAFCRKNLSHYMVPKKVVFVAELPK-ANGKVQKFMLREQAK 541

Query: 544 LFVVSEKRVGGGNKSGRQDGGRRQDYGEHVLAMSR 579
              VSEKR+   +  G + G RR DYGEHV+AMSR
Sbjct: 542 YLEVSEKRI--VSNMGNKSGNRRSDYGEHVIAMSR 571

BLAST of Tan0022147 vs. ExPASy TrEMBL
Match: A0A5A7TXW0 (Butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold284G00160 PE=4 SV=1)

HSP 1 Score: 1008.8 bits (2607), Expect = 9.0e-291
Identity = 491/579 (84.80%), Postives = 533/579 (92.06%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           ME LLKCDANY +L+PITFLKRASAFY+DRTSIIYEGTRFTW QTYERCCRLASSL RL+
Sbjct: 1   MEMLLKCDANYTALTPITFLKRASAFYADRTSIIYEGTRFTWNQTYERCCRLASSLRRLS 60

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           ++KN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLDVKNIA+ILRH+E+KI FVDYQ
Sbjct: 61  VSKNHVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVKNIALILRHSEAKIFFVDYQ 120

Query: 121 YIQQAKDALRLLMAESSPLPLVVVIDDIDSPTGARLGNLEYEQLIHDGDVGFVPIEIDDE 180
           YIQ+AKDALRLL+AES  +PLVVVIDDID+PTGARLGNLEYEQLIHDGD  F P+E+DDE
Sbjct: 121 YIQEAKDALRLLVAESFTIPLVVVIDDIDTPTGARLGNLEYEQLIHDGDANFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVGWPVNV 300
           WGIAARGGTN+CMRNTTA DI+RNI+LH VTHMCCAPIVF IILE D G+R+RV WPVNV
Sbjct: 241 WGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFRIILEADNGDRRRVAWPVNV 300

Query: 301 LTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKLKARQG 360
           LTGGAPPP  LLEKMEALGFHITHAYGLTEATGPALVCEWQ+KWNVLP D+QA LKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQG 360

Query: 361 ISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNL TME VPHDGRT+GEIVLRGSS+MKGYFKDP ATAAA KNGWFLTG
Sbjct: 361 ISILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPEATAAAFKNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           D+GVI+PDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAIRGGEQGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTSTGKIQKFKLRE 540
           CAFV + GG  GG+GRVSEAE+IAFCRK+++H+MVPKKVVF+AELPK + GK+QKF LRE
Sbjct: 481 CAFVVLHGG--GGSGRVSEAEMIAFCRKNLSHYMVPKKVVFMAELPK-ANGKVQKFMLRE 540

Query: 541 QAKLFVVSEKRVGGGNKSGRQDGGRRQDYG-EHVLAMSR 579
           QAK   VSEKR    +  G + G RR DYG +HV+AMSR
Sbjct: 541 QAKYLEVSEKR--RVSNLGNKSGNRRSDYGDQHVIAMSR 574

BLAST of Tan0022147 vs. ExPASy TrEMBL
Match: A0A1S3CIM1 (butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC103501376 PE=4 SV=1)

HSP 1 Score: 1008.8 bits (2607), Expect = 9.0e-291
Identity = 491/579 (84.80%), Postives = 533/579 (92.06%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           ME LLKCDANY +L+PITFLKRASAFY+DRTSIIYEGTRFTW QTYERCCRLASSL RL+
Sbjct: 1   MEMLLKCDANYTALTPITFLKRASAFYADRTSIIYEGTRFTWNQTYERCCRLASSLRRLS 60

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           ++KN VVSVLAPNVPALYEMHFAVPMAGA+LNTINTRLDVKNIA+ILRH+E+KI FVDYQ
Sbjct: 61  VSKNHVVSVLAPNVPALYEMHFAVPMAGAILNTINTRLDVKNIALILRHSEAKIFFVDYQ 120

Query: 121 YIQQAKDALRLLMAESSPLPLVVVIDDIDSPTGARLGNLEYEQLIHDGDVGFVPIEIDDE 180
           YIQ+AKDALRLL+AES  +PLVVVIDDID+PTGARLGNLEYEQLIHDGD  F P+E+DDE
Sbjct: 121 YIQEAKDALRLLVAESFTIPLVVVIDDIDTPTGARLGNLEYEQLIHDGDANFTPVEVDDE 180

Query: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240
           WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT
Sbjct: 181 WDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNGWTFT 240

Query: 241 WGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVGWPVNV 300
           WGIAARGGTN+CMRNTTA DI+RNI+LH VTHMCCAPIVF IILE D G+R+RV WPVNV
Sbjct: 241 WGIAARGGTNICMRNTTASDIFRNINLHRVTHMCCAPIVFRIILEADNGDRRRVAWPVNV 300

Query: 301 LTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKLKARQG 360
           LTGGAPPP  LLEKMEALGFHITHAYGLTEATGPALVCEWQ+KWNVLP D+QA LKARQG
Sbjct: 301 LTGGAPPPAALLEKMEALGFHITHAYGLTEATGPALVCEWQEKWNVLPADQQANLKARQG 360

Query: 361 ISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNGWFLTG 420
           ISILTLADVDVKNL TME VPHDGRT+GEIVLRGSS+MKGYFKDP ATAAA KNGWFLTG
Sbjct: 361 ISILTLADVDVKNLKTMESVPHDGRTTGEIVLRGSSLMKGYFKDPEATAAAFKNGWFLTG 420

Query: 421 DVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRWGESP 480
           D+GVI+PDGYLEIKDRSKDVIISGGENISSVE+ETVLYRHPQVAEAAVVAMPHPRWGESP
Sbjct: 421 DIGVIYPDGYLEIKDRSKDVIISGGENISSVEVETVLYRHPQVAEAAVVAMPHPRWGESP 480

Query: 481 CAFVAIRGGEQGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTSTGKIQKFKLRE 540
           CAFV + GG  GG+GRVSEAE+IAFCRK+++H+MVPKKVVF+AELPK + GK+QKF LRE
Sbjct: 481 CAFVVLHGG--GGSGRVSEAEMIAFCRKNLSHYMVPKKVVFMAELPK-ANGKVQKFMLRE 540

Query: 541 QAKLFVVSEKRVGGGNKSGRQDGGRRQDYG-EHVLAMSR 579
           QAK   VSEKR    +  G + G RR DYG +HV+AMSR
Sbjct: 541 QAKYLEVSEKR--RVSNLGNKSGNRRSDYGDQHVIAMSR 574

BLAST of Tan0022147 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 707.6 bits (1825), Expect = 8.3e-204
Identity = 346/558 (62.01%), Postives = 421/558 (75.45%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M+ L+ C+AN   L+PITFLKRAS  Y +RTSIIY  TRFTW QTY+RCCRLA+SL  LN
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLLSLN 60

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           I +NDVVS+LAPNVPA+YEMHF+VPM GAVLN INTRLD K IAIILRHAE KILFVDY+
Sbjct: 61  ITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDYE 120

Query: 121 YIQQAKDALRLLMA-ESSPLPLVVVIDDIDSPTGARLGNLEYEQLIHDGD----VGFVPI 180
           +    ++ LRL+   +S P P +++I++IDS T      L+YE LI  G+          
Sbjct: 121 FAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLIRKGEPTPSSSASMF 180

Query: 181 EIDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            + +E D I+LNYTSGTT+ PKGVV SH+GA+LS LS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGIFPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVG 300
           GWT TW +AARGGTNVC+R+ TA +IY+NI LH VTHM C P VF  +LE    ++    
Sbjct: 241 GWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSRTDQSPKS 300

Query: 301 WPVNVLTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKL 360
            PV VLTGG+ PP  L++K+E LGFH+ H YGLTEATGP L CEWQ +WN LP  +Q +L
Sbjct: 301 SPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLPEHQQIEL 360

Query: 361 KARQGISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNG 420
           + RQG+  LTLADVDVKN  T+E VP DG+T GEIV++GSS+MKGY K+P AT+ A K+G
Sbjct: 361 QQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKATSEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  TGD+GVIHPDGY+EIKDRSKD+IISGGENISS+E+E VLY + +V EAAVVAMPHP 
Sbjct: 421 WLNTGDIGVIHPDGYVEIKDRSKDIIISGGENISSIEVEKVLYMYQEVLEAAVVAMPHPL 480

Query: 481 WGESPCAFVAIRGGEQGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTSTGKIQK 540
           WGE+PCAFV ++ GE+G     SE ++I +CR++M HFM PKKVVF  ELPK S GKI K
Sbjct: 481 WGETPCAFVVLKKGEEGLV--TSEGDLIKYCRENMPHFMCPKKVVFFQELPKNSNGKILK 540

Query: 541 FKLREQAKLFVVSEKRVG 554
            KLR+ AK  VV E   G
Sbjct: 541 SKLRDIAKALVVREDDAG 556

BLAST of Tan0022147 vs. TAIR 10
Match: AT1G65890.1 (acyl activating enzyme 12 )

HSP 1 Score: 694.5 bits (1791), Expect = 7.3e-200
Identity = 341/560 (60.89%), Postives = 422/560 (75.36%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M+ L  C+AN   L+PITFLKRAS  Y +RTSIIY  TRFTW QTY+RCCRLA+SL  LN
Sbjct: 1   MDNLALCEANNVPLTPITFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           I KNDVVSV+APN PA+YEMHFAVPMAGAVLN INTRLD  +IA ILRHA+ KILF+   
Sbjct: 61  IGKNDVVSVVAPNTPAMYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFIYRS 120

Query: 121 YIQQAKDALRLLMAESSPLPL-VVVIDDIDSPTGARLGNLEYEQLIHDGDVGFVPIE--- 180
           +   A++ L+LL +E S L L V+ I +ID P        +YE LI  G+   + +    
Sbjct: 121 FEPLAREILQLLSSEDSNLNLPVIFIHEIDFPKRVSSEESDYECLIQRGEPTPLLLARMF 180

Query: 181 -IDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            I DE D I+LNYTSGTT+ PKGVV SHRGA+LSTLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 CIQDEHDPISLNYTSGTTADPKGVVISHRGAYLSTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVG 300
           GWTFTWG AARGGT+VCMR+ TA +IY+NI +H+VTHMCC P VF+I+L+ +  +     
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSHRS 300

Query: 301 WPVNVLTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKL 360
            PV+VLTGG+PPP  L++K++ LGF + HAYGLTEATGP L CEWQ +WN LP ++Q +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGLTEATGPVLFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNG 420
           KARQG+SIL L +VDV+N +T E VP DG+T GEIV++GSSIMKGY K+P AT  A K+G
Sbjct: 361 KARQGLSILGLTEVDVRNKETQESVPRDGKTMGEIVMKGSSIMKGYLKNPKATYEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  +GDVGVIHPDG++EIKDRSKD+IISGGENISSVE+E ++Y++P+V E AVVAMPHP 
Sbjct: 421 WLNSGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENIIYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFVAIRGGEQGGAGR-----VSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTST 540
           WGE+PCAFV +  GE     R       E ++I +CR+++ HFM P+KVVF+ ELPK   
Sbjct: 481 WGETPCAFVVLEKGETNNEDREDKLVTKERDLIEYCRENLPHFMCPRKVVFLDELPKNGN 540

Query: 541 GKIQKFKLREQAKLFVVSEK 551
           GKI K KLR+ AK  V  ++
Sbjct: 541 GKILKPKLRDIAKGLVAEDE 560

BLAST of Tan0022147 vs. TAIR 10
Match: AT1G65880.1 (benzoyloxyglucosinolate 1 )

HSP 1 Score: 692.6 bits (1786), Expect = 2.8e-199
Identity = 344/561 (61.32%), Postives = 422/561 (75.22%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           M+ L  C+AN   L+P+TFLKRAS  Y +RTSIIY  TRFTW QTY+RCCRLA+SL  LN
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLISLN 60

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           I+KNDVVSV+APN PALYEMHFAVPMAGAVLN INTRLD  +IA ILRHA+ KILF+D  
Sbjct: 61  ISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDRS 120

Query: 121 YIQQAKDALRLLMAESSPLPL-VVVIDDIDSPTGARLGNLEYEQLIHDGD----VGFVPI 180
           +   A+++L LL +E S L L V+ I + D P  A    L+YE LI  G+    +     
Sbjct: 121 FEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLIQRGEPTPSMVARMF 180

Query: 181 EIDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCN 240
            I DE D I+LNYTSGTT+ PKGVV SHRGA+L TLS ++GWEMG  PVYLWTLPMFHCN
Sbjct: 181 RIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTCPVYLWTLPMFHCN 240

Query: 241 GWTFTWGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVG 300
           GWTFTWG AARGGT+VCMR+ TA +IY+NI +H+VTHMCC P VF+I+L+ +  +     
Sbjct: 241 GWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGNSLDLSPRS 300

Query: 301 WPVNVLTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKL 360
            PV+VLTGG+PPP  L++K++ LGF + HAYG TEATGP L CEWQ +WN LP ++Q +L
Sbjct: 301 GPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWNRLPENQQMEL 360

Query: 361 KARQGISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNG 420
           KARQGISIL LADVDVKN +T +  P DG+T GEI+++GSSIMKGY K+P AT  A K+G
Sbjct: 361 KARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNPKATFEAFKHG 420

Query: 421 WFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPR 480
           W  TGDVGVIHPDG++EIKDRSKD+IISGGENISSVE+E VLY++P+V E AVVAMPHP 
Sbjct: 421 WLNTGDVGVIHPDGHVEIKDRSKDIIISGGENISSVEVENVLYKYPKVLETAVVAMPHPT 480

Query: 481 WGESPCAFVAIRGGE------QGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTS 540
           WGE+PCAFV +   E      +    +  E  +I +CR+++ HFM P+KVVF+ ELPK  
Sbjct: 481 WGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPRKVVFLEELPKNG 540

Query: 541 TGKIQKFKLREQAKLFVVSEK 551
            GKI K KLR+ AK  VV ++
Sbjct: 541 NGKILKPKLRDIAKGLVVEDE 561

BLAST of Tan0022147 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 580.1 bits (1494), Expect = 2.0e-165
Identity = 280/555 (50.45%), Postives = 391/555 (70.45%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIYEGTRFTWRQTYERCCRLASSLCRLN 60
           ME  +K  ANY  L+PI+FL R++  Y+DR SI+Y   ++TWRQT +RC R+AS+L +L 
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASALSQLG 62

Query: 61  IAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDYQ 120
           I+  DVVSVLAPNVPA+ E+HF VPMAGA+L T+N R D   +A++LRH+ +K++F D+Q
Sbjct: 63  ISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQ 122

Query: 121 YIQQAKDALRLLMAESSPLPLVVVIDDIDSPTGARLGN----LEYEQLIHDGDVGFVPIE 180
           ++Q A+ A  +L  +   +P++V+I +  + + +R       +EYE ++  G   F  I 
Sbjct: 123 FLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIR 182

Query: 181 IDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFHCNG 240
             DE D+I++NYTSGTTS+PKGVVYSHRGA+L++L+ V+  EM ++P YLWT PMFHCNG
Sbjct: 183 PTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHSSPTYLWTNPMFHCNG 242

Query: 241 WTFTWGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQRVGW 300
           W   WG+ A GGTN+C+RN TA  I+ NI  H VTHM  AP + ++I+     E++ +  
Sbjct: 243 WCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPG 302

Query: 301 PVNVLTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQAKLK 360
            V+ +TG APPP  ++ KME LGF + H+YGLTE  GP  +C W+ +W+ LP +EQAK+K
Sbjct: 303 KVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMK 362

Query: 361 ARQGISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALKNGW 420
           ARQG++ L L ++ VK+  TM  +P DG T GE+V RG+++M GY K+P AT  A K GW
Sbjct: 363 ARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGW 422

Query: 421 FLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPHPRW 480
           F +GD+GV HPDGY+E+KDRSKD+IISGGENISS+E+E+ L+ HP V EAAVVA P   W
Sbjct: 423 FWSGDLGVKHPDGYIELKDRSKDIIISGGENISSIEVESTLFTHPCVLEAAVVARPDEYW 482

Query: 481 GESPCAFVAIRGGEQGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTSTGKIQKF 540
           GE+ CAFV ++ G      + S  E+I++CR  + H+M P+ +VF  +LPKTSTGK+QKF
Sbjct: 483 GETACAFVKLKDG-----SKASAEELISYCRDRLPHYMAPRSIVF-EDLPKTSTGKVQKF 542

Query: 541 KLREQAKLFVVSEKR 552
            LR +AK  V   K+
Sbjct: 543 VLRTKAKALVSLSKK 551

BLAST of Tan0022147 vs. TAIR 10
Match: AT5G16370.1 (acyl activating enzyme 5 )

HSP 1 Score: 579.7 bits (1493), Expect = 2.6e-165
Identity = 292/550 (53.09%), Postives = 381/550 (69.27%), Query Frame = 0

Query: 1   MEKLLKCDANYASLSPITFLKRASAFYSDRTSIIY-EGTRFTWRQTYERCCRLASSLCRL 60
           ME++  C AN   L+PI FL+RA+  Y D TSI+Y   T +TWR+T  RC R+ASSL  +
Sbjct: 1   MEQMKPCAANSPPLTPIGFLERAATVYGDCTSIVYGSNTVYTWRETNLRCLRVASSLSSI 60

Query: 61  NIAKNDVVSVLAPNVPALYEMHFAVPMAGAVLNTINTRLDVKNIAIILRHAESKILFVDY 120
            I ++DVVSVL+PN PA+YE+ FAVPM+GA+LN INTRLD + ++++LRH  SK+LFVD 
Sbjct: 61  GIGRSDVVSVLSPNTPAMYELQFAVPMSGAILNNINTRLDARTVSVLLRHCGSKLLFVDV 120

Query: 121 QYIQQAKDALRLLMAESSPLPLVVVIDDIDSPTG------ARLGNLEYEQLIHDGDVGFV 180
             +  A +A+ ++  +    P++V I D +   G          +  Y+ LIH GD+ F 
Sbjct: 121 FSVDLAVEAISMMTTDP---PILVFIADKEEEGGDADVADRTKFSYTYDDLIHRGDLDFK 180

Query: 181 PIEIDDEWDSIALNYTSGTTSAPKGVVYSHRGAFLSTLSLVMGWEMGNAPVYLWTLPMFH 240
            I  + EWD + LNYTSGTTSAPKGVV+ HRG F+ ++  ++ W +   PVYLWTLP+FH
Sbjct: 181 WIRPESEWDPVVLNYTSGTTSAPKGVVHCHRGIFVMSIDSLIDWTVPKNPVYLWTLPIFH 240

Query: 241 CNGWTFTWGIAARGGTNVCMRNTTAFDIYRNIHLHHVTHMCCAPIVFSIILEPDGGERQR 300
            NGW++ WGIAA GGTNVC+R   A  IYR I  H VTHMC AP+V +++   +  E Q 
Sbjct: 241 ANGWSYPWGIAAVGGTNVCLRKFDAPLIYRLIRDHGVTHMCGAPVVLNMLSATN--EFQP 300

Query: 301 VGWPVNVLTGGAPPPPPLLEKMEALGFHITHAYGLTEATGPALVCEWQQKWNVLPVDEQA 360
           +  PVN+LT GAPPP  +L + E++GF I+H YGLTE  G  + C W+ +WN LP  ++A
Sbjct: 301 LNRPVNILTAGAPPPAAVLLRAESIGFVISHGYGLTETAGLNVSCAWKPQWNRLPASDRA 360

Query: 361 KLKARQGISILTLADVDVKNLDTMEGVPHDGRTSGEIVLRGSSIMKGYFKDPAATAAALK 420
           +LKARQG+  +   ++DV + ++   V  +G T GEIV+RGSSIM GY KDP  T  ALK
Sbjct: 361 RLKARQGVRTVGFTEIDVVDPESGRSVERNGETVGEIVMRGSSIMLGYLKDPVGTEKALK 420

Query: 421 NGWFLTGDVGVIHPDGYLEIKDRSKDVIISGGENISSVELETVLYRHPQVAEAAVVAMPH 480
           NGWF TGDVGVIH DGYLEIKDRSKD+II+GGEN+SSVE+ETVLY +P V E AVVA P 
Sbjct: 421 NGWFYTGDVGVIHSDGYLEIKDRSKDIIITGGENVSSVEVETVLYTNPAVNEVAVVARPD 480

Query: 481 PRWGESPCAFVAIRGGEQGGAGRVSEAEIIAFCRKSMAHFMVPKKVVFVAELPKTSTGKI 540
             WGE+PCAFV+++ G      R +E E+I +CRK M  +MVPK V FV ELPKTSTGK+
Sbjct: 481 VFWGETPCAFVSLKSGL---TQRPTEVEMIEYCRKKMPKYMVPKTVSFVDELPKTSTGKV 540

Query: 541 QKFKLREQAK 544
            KF LRE AK
Sbjct: 541 MKFVLREIAK 542

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
I3PB361.0e-21465.65Trans-cinnamate:CoA ligase, peroxisomal OS=Petunia hybrida OX=4102 GN=CNL PE=1 S... [more]
Q9C8D41.2e-20262.01Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11... [more]
Q9SS001.0e-19860.89Probable acyl-activating enzyme 12, peroxisomal OS=Arabidopsis thaliana OX=3702 ... [more]
Q9SS013.9e-19861.32Benzoate--CoA ligase, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE20 PE=1 ... [more]
F4HUK62.8e-16450.45Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022923630.13.6e-30287.37butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita moschata][more]
XP_023513711.14.0e-30187.02butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita pepo subsp. pepo][more]
KAG6602004.15.2e-30187.20Benzoate--CoA ligase, peroxisomal, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022990938.11.7e-29986.51butyrate--CoA ligase AAE11, peroxisomal-like [Cucurbita maxima][more]
XP_011655134.11.7e-29685.29butyrate--CoA ligase AAE11, peroxisomal [Cucumis sativus] >KGN51049.2 hypothetic... [more]
Match NameE-valueIdentityDescription
A0A6J1ECD91.8e-30287.37butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1JUR48.2e-30086.51butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A0A0KT409.3e-29685.39Acyl:coa ligase acetate-coa synthetase-like protein OS=Cucumis sativus OX=3659 G... [more]
A0A5A7TXW09.0e-29184.80Butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3CIM19.0e-29184.80butyrate--CoA ligase AAE11, peroxisomal-like OS=Cucumis melo OX=3656 GN=LOC10350... [more]
Match NameE-valueIdentityDescription
AT1G66120.18.3e-20462.01AMP-dependent synthetase and ligase family protein [more]
AT1G65890.17.3e-20060.89acyl activating enzyme 12 [more]
AT1G65880.12.8e-19961.32benzoyloxyglucosinolate 1 [more]
AT1G20560.12.0e-16550.45acyl activating enzyme 1 [more]
AT5G16370.12.6e-16553.09acyl activating enzyme 5 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 452..532
e-value: 7.1E-24
score: 84.6
NoneNo IPR availableGENE3D3.30.300.30coord: 439..546
e-value: 1.7E-31
score: 110.9
NoneNo IPR availablePANTHERPTHR43859ACYL-ACTIVATING ENZYMEcoord: 1..551
NoneNo IPR availablePANTHERPTHR43859:SF2BUTYRATE--COA LIGASE AAE11, PEROXISOMALcoord: 1..551
NoneNo IPR availableCDDcd12118ttLC_FACS_AEE21_likecoord: 11..540
e-value: 0.0
score: 817.308
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 14..542
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 22..443
e-value: 3.1E-93
score: 312.7
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 3..438
e-value: 2.2E-95
score: 321.7
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 186..197

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0022147.1Tan0022147.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
molecular_function GO:0016874 ligase activity