Tan0022060 (gene) Snake gourd v1

Overview


Name	Tan0022060
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	DNA-directed RNA polymerase III subunit rpc4
Location	LG09: 63277599 .. 63279488 (+)
RNA-Seq Expression	Tan0022060
Synteny	Tan0022060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAGTTGTAGATTGATCCGTGGAGTTCTCATGATATGACTCAACATATTAGTTATGCATAATGTGGTTTTGCCCCAACTGCACCACCTAGCACATCGGTGGAATGGGGTGAACTTTTGTCTGTTACCGTTAACCCTGCATACAATTGAAATCTGTTTGCACAATGTTTAAAGATATTTAGTTCTTCTAACATGTCTCCGTTTCTTGCATGCCTGTCATTTAAATATGCAGCGGCACCAACACAAGTTGCATTTGGTTCTGGAGGTACATCAAGCACCATAAGATCATTTGGTCTTTCAAAAGCTGGAAATAGACCCAGAAATGAAGGTTGGCTACTAAACTACTAAAATCTACACTACTCAAGATGTAATTGCTTGAATTATTGTACGTGAGATGTCGAGGCCCTAAACTGAAAGGTGATCATGCAGATGGAACTCTTCCGTCGAGTGCAAGCAAGGAGTATGTGGAACCATGGGTCAGTAAAATATACAATCCTCAATTATTTTTCCTTTGCAATTCCTGTTTGCATCTGCTTTCTCCATTAGTATGGCAATTTAGTAATTTGATTTTCATTTTACTGCAGGATTATTACAGTTATTATCCTGTAACTCTTCCTTTACGGAGGCCATATTCAGGAAATCCAGGTATCTTTGTAAGCTAATCTTATTTACAAGAACTAAAAGTAAAATACTGCAGTGGACAACTGGATTATTCTGAAATACACTGTTTTCGACGTGTGTGGTTTGCATACTTCGTATCACTCATTGACAGTTTTCCTGGTGAATCCATGCAAAATGTACCAGCGTCTTCTGCATCAATTATCTGCTCTAAGTTTTGTATGCATTACTTTATAGATTCTCTTAACGAGGAAGAATTTGGAGAGGCTTCAGAAACCATAACCTATGATGAAAATACAACAACTCCTGCACTGGATCTTGGTTTAATGGTTAGTCACTGGATTATGTGGATGGCCTAGCTTGAGCCGCTTGTTTCCAATTTTACGCTTAATTGTTTATTTGGCAGGAGGATAATCCAGAAACAGACATATTTTTTCTACAATTACCGCCTATGGTGCCTGTGATAAAGCAATCTTCTACTCTAGAAGGCATGGAAGCAGCCAACAGCTTGGAACAAAGGAAAGGTTCACAACCTCGACAGAAAACCTGCTCGCTGAACGAGTTACCTTCTGGCTCCATTGGTAAATTGCTTGTGTATAGGAGTGGCGCTGTCAAGTTAAAGCTTGGCGATATTGTATATGATGTGAGTTTTTTTAATCATAACATGAGCATGCCAAGTTCGTCGTTGTCTACTTAATTATCAACTCAATTGATCTCTTGAGATGAAATTTTCTATTAGAAGCATGGAATCTCTGTCTCAGCTTTCTGAAACTCTTCACTTGATGAAAAGATTGCACTACTTACGTTGTAGGAAGTTCTTATTGATTGATTTTGTGTTCCTTCTCACATCAGTTCTAAAATATGAGCAGGTTTCTTCTGGAATGGATTGTGGCTTTGCACAAGACGTTGCAGCTATCAATGTGGAGGGGAAGCATTGCTGCATTGTTGGAGAGCTCAGCAAGCGTGCCATTTTAACACCAGATGTGGACTCAATGTTAAAGAATATCGAGGATTTATGATTATTTTAAGATGGAATTTTTTCATAGACATCAATGTAGAGAAAATGACGGGAGTGATATTCTTTATCAAAGCTACAAGAGGATATAACAAAAAAGATAGCAGCCAGCAGAAAGAACTACACTGGTTCAAGAAAAACGGAGGAGTGACTATTTTCTCTCTTGTTTAGCCCCGAGTGCCTAGATTTTTTAAATTATGAACAATTGTCGTGTGATTAACTAGATCAATTATATTAATTACAATTTTGACAAGAGTAAT

mRNA sequence

AAGTTGTAGATTGATCCGTGGAGTTCTCATGATATGACTCAACATATTAGTTATGCATAATGTGGTTTTGCCCCAACTGCACCACCTAGCACATCGGTGGAATGGGGTGAACTTTTGTCTGTTACCGTTAACCCTGCATACAATTGAAATCTGTTTGCACAATGTTTAAAGATATTTAGTTCTTCTAACATGTCTCCGTTTCTTGCATGCCTGTCATTTAAATATGCAGCGGCACCAACACAAGTTGCATTTGGTTCTGGAGGTACATCAAGCACCATAAGATCATTTGGTCTTTCAAAAGCTGGAAATAGACCCAGAAATGAAGATGGAACTCTTCCGTCGAGTGCAAGCAAGGAGTATGTGGAACCATGGGATTATTACAGTTATTATCCTGTAACTCTTCCTTTACGGAGGCCATATTCAGGAAATCCAGATTCTCTTAACGAGGAAGAATTTGGAGAGGCTTCAGAAACCATAACCTATGATGAAAATACAACAACTCCTGCACTGGATCTTGGTTTAATGGAGGATAATCCAGAAACAGACATATTTTTTCTACAATTACCGCCTATGGTGCCTGTGATAAAGCAATCTTCTACTCTAGAAGGCATGGAAGCAGCCAACAGCTTGGAACAAAGGAAAGGTTCACAACCTCGACAGAAAACCTGCTCGCTGAACGAGTTACCTTCTGGCTCCATTGGTAAATTGCTTGTGTATAGGAGTGGCGCTGTCAAGTTAAAGCTTGGCGATATTGTATATGATGTTTCTTCTGGAATGGATTGTGGCTTTGCACAAGACGTTGCAGCTATCAATGTGGAGGGGAAGCATTGCTGCATTGTTGGAGAGCTCAGCAAGCGTGCCATTTTAACACCAGATGTGGACTCAATGTTAAAGAATATCGAGGATTTATGATTATTTTAAGATGGAATTTTTTCATAGACATCAATGTAGAGAAAATGACGGGAGTGATATTCTTTATCAAAGCTACAAGAGGATATAACAAAAAAGATAGCAGCCAGCAGAAAGAACTACACTGGTTCAAGAAAAACGGAGGAGTGACTATTTTCTCTCTTGTTTAGCCCCGAGTGCCTAGATTTTTTAAATTATGAACAATTGTCGTGTGATTAACTAGATCAATTATATTAATTACAATTTTGACAAGAGTAAT

Coding sequence (CDS)

ATGTCTCCGTTTCTTGCATGCCTGTCATTTAAATATGCAGCGGCACCAACACAAGTTGCATTTGGTTCTGGAGGTACATCAAGCACCATAAGATCATTTGGTCTTTCAAAAGCTGGAAATAGACCCAGAAATGAAGATGGAACTCTTCCGTCGAGTGCAAGCAAGGAGTATGTGGAACCATGGGATTATTACAGTTATTATCCTGTAACTCTTCCTTTACGGAGGCCATATTCAGGAAATCCAGATTCTCTTAACGAGGAAGAATTTGGAGAGGCTTCAGAAACCATAACCTATGATGAAAATACAACAACTCCTGCACTGGATCTTGGTTTAATGGAGGATAATCCAGAAACAGACATATTTTTTCTACAATTACCGCCTATGGTGCCTGTGATAAAGCAATCTTCTACTCTAGAAGGCATGGAAGCAGCCAACAGCTTGGAACAAAGGAAAGGTTCACAACCTCGACAGAAAACCTGCTCGCTGAACGAGTTACCTTCTGGCTCCATTGGTAAATTGCTTGTGTATAGGAGTGGCGCTGTCAAGTTAAAGCTTGGCGATATTGTATATGATGTTTCTTCTGGAATGGATTGTGGCTTTGCACAAGACGTTGCAGCTATCAATGTGGAGGGGAAGCATTGCTGCATTGTTGGAGAGCTCAGCAAGCGTGCCATTTTAACACCAGATGTGGACTCAATGTTAAAGAATATCGAGGATTTATGA

Protein sequence

MSPFLACLSFKYAAAPTQVAFGSGGTSSTIRSFGLSKAGNRPRNEDGTLPSSASKEYVEPWDYYSYYPVTLPLRRPYSGNPDSLNEEEFGEASETITYDENTTTPALDLGLMEDNPETDIFFLQLPPMVPVIKQSSTLEGMEAANSLEQRKGSQPRQKTCSLNELPSGSIGKLLVYRSGAVKLKLGDIVYDVSSGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKNIEDL

Homology

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BLAST of Tan0022060 vs. ExPASy Swiss-Prot
Match: Q5E9Z7 (DNA-directed RNA polymerase III subunit RPC4 OS=Bos taurus OX=9913 GN=POLR3D PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 9.6e-10
Identity = 51/208 (24.52%), Postives = 92/208 (44.23%), Query Frame = 0


Query: 42  PRNEDGTLPSSASKEYVEPWDYYSYYPVTLPLR--RPYSGNPDSLNEEEFGEASETITYD 101
           P+ ED  +   A K   EP D      +  PLR  R   G P  ++  E           
Sbjct: 205 PKEEDMEVDMPAVKVKEEPRDEDEEAKMKAPLRAARKIPGLPKDVSVAEL---------- 264

Query: 102 ENTTTPALDLGLMEDNPETDIFFLQLPPMV----------PVIKQSSTLEGMEAANSLEQ 161
                   +L L +   E ++ FLQLP  +          P+  +  + +G       E+
Sbjct: 265 ------LRELSLTQ---EEELLFLQLPDSLPGQPPTQDIKPIKTEVQSEDGQMVVIKQEK 324

Query: 162 RKGSQPRQKTCSLNELPSGSIGKLLVYRSGAVKLKLGDIVYDVSSGMDCGFAQDVAAINV 221
            + ++  + TC+L +L  G +GKLL+ +SG V+L LG +  DV+ G  C F Q++ ++ +
Sbjct: 325 DREARLAENTCTLADLTEGQVGKLLIRKSGKVQLLLGKVTLDVTMGTTCSFLQELVSVGL 384

Query: 222 EGKH---CCIVGELSKRAILTPDVDSML 235
                    ++G +  + + +P+ +S+L
Sbjct: 385 GDSRTGDMTVLGHIKHKLVCSPNFESLL 393

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BLAST of Tan0022060 vs. ExPASy Swiss-Prot
Match: Q91WD1 (DNA-directed RNA polymerase III subunit RPC4 OS=Mus musculus OX=10090 GN=Polr3d PE=2 SV=2)

HSP 1 Score: 65.1 bits (157), Expect = 1.3e-09
Identity = 53/208 (25.48%), Postives = 90/208 (43.27%), Query Frame = 0


Query: 42  PRNEDGTLPSSASKEYVEPWDYYSYYPVTLPLR--RPYSGNPDSLNEEEFGEASETITYD 101
           P+ ED  +   A K   EP D      V  P R  R   G P  ++  E           
Sbjct: 205 PKEEDMEVDVPAVKVKEEPRDEEEEAKVKAPPRAARKTPGLPKDVSVAEL---------- 264

Query: 102 ENTTTPALDLGLMEDNPETDIFFLQLPPMV----------PVIKQSSTLEGMEAANSLEQ 161
                   +L LM+D    ++ FLQLP  +          PV  +    +G       E+
Sbjct: 265 ------LRELSLMKDE---ELLFLQLPDTLPGQPPTQDIKPVKTEVQGEDGQMVVIKQEK 324

Query: 162 RKGSQPRQKTCSLNELPSGSIGKLLVYRSGAVKLKLGDIVYDVSSGMDCGFAQDVAAINV 221
            + ++  +  C+L +L  G +GKLL+ +SG V+L LG +  DV+ G  C F Q++ ++ +
Sbjct: 325 DREARLAENACTLADLTEGQVGKLLIRKSGKVQLLLGKVTLDVTMGTTCSFLQELVSVGL 384

Query: 222 EGK---HCCIVGELSKRAILTPDVDSML 235
                    ++G +  + + +PD +S+L
Sbjct: 385 GDSRTGEMTVLGHVKHKLVCSPDFESLL 393

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BLAST of Tan0022060 vs. ExPASy Swiss-Prot
Match: P05423 (DNA-directed RNA polymerase III subunit RPC4 OS=Homo sapiens OX=9606 GN=POLR3D PE=1 SV=2)

HSP 1 Score: 62.0 bits (149), Expect = 1.1e-08
Identity = 34/131 (25.95%), Postives = 66/131 (50.38%), Query Frame = 0


Query: 117 ETDIFFLQLPPMV----------PVIKQSSTLEGMEAANSLEQRKGSQPRQKTCSLNELP 176
           E ++ FLQLP  +          P+  +    +G       E+ + ++  +  C+L +L 
Sbjct: 263 EEELLFLQLPDTLPGQPPTQDIKPIKTEVQGEDGQVVLIKQEKDREAKLAENACTLADLT 322

Query: 177 SGSIGKLLVYRSGAVKLKLGDIVYDVSSGMDCGFAQDVAAINVEGK---HCCIVGELSKR 235
            G +GKLL+ +SG V+L LG +  DV+ G  C F Q++ ++ +         ++G +  +
Sbjct: 323 EGQVGKLLIRKSGRVQLLLGKVTLDVTMGTACSFLQELVSVGLGDSRTGEMTVLGHVKHK 382

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BLAST of Tan0022060 vs. ExPASy Swiss-Prot
Match: O74857 (DNA-directed RNA polymerase III subunit rpc4 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=rpc53 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 2.9e-06
Identity = 45/173 (26.01%), Postives = 82/173 (47.40%), Query Frame = 0


Query: 84  LNEEEFGEASETITYDENTTTPALDLGL-----MEDNPETDIFFLQLPPMVP-------- 143
           +N+E+  E  E +  D  +   A  LGL     MED+ +  +  +Q P  +P        
Sbjct: 161 INKED--EEQERVALDLQSL--AQHLGLTEQEGMEDHFKDQMVLMQFPDKLPRFMGDADV 220

Query: 144 -VIKQSSTLEGMEAANSLEQRKGSQP---RQKTCSLNEL----------PSGSIGKLLVY 203
             +  SS ++  EA    +  +   P    ++  ++++L          P+G IG L V+
Sbjct: 221 DPVWPSSEVKEEEAGEKTDVEEKKDPDKHGRENGNIDDLTSLPYPAFHPPAGQIGVLRVH 280

Query: 204 RSGAVKLKLGDIVYDVSSGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPD 230
           +SG   L++G + + V SG DC F Q++A ++ + K    +G   +R I++PD
Sbjct: 281 QSGKTTLEMGGVNFVVQSGSDCLFLQEIAVVDYDSKRIWNLGSFERRMIVSPD 329

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BLAST of Tan0022060 vs. ExPASy Swiss-Prot
Match: P25441 (DNA-directed RNA polymerase III subunit RPC4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RPC53 PE=1 SV=2)

HSP 1 Score: 53.5 bits (127), Expect = 3.8e-06
Identity = 35/128 (27.34%), Postives = 67/128 (52.34%), Query Frame = 0


Query: 112 MEDNPETDIFFLQLPPMVPVIKQSST---LEGMEAANSLEQRKGSQPRQK----TCSLNE 171
           M + PE  + F QLP  +P  ++ +     E ME   S   +K    ++K      S  E
Sbjct: 297 MNNKPERFMVF-QLPTRLPAFERPAVKEEKEDMETQASDPSKKKKNIKKKDTKDALSTRE 356

Query: 172 LPSGSIGKLLVYRSGAVKLKLGDIVYDVSSGMDCGFAQDVAAINV--EGKHCCIVGELSK 231
           L +G +G + V++SG + +K+G++V D+  G +  F QDV A+++  +     ++G +  
Sbjct: 357 L-AGKVGSIRVHKSGKLSVKIGNVVMDIGKGAETTFLQDVIALSIADDASSAELLGRVDG 416

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BLAST of Tan0022060 vs. NCBI nr
Match: XP_038878504.1 (uncharacterized protein LOC120070724 [Benincasa hispida] >XP_038878505.1 uncharacterized protein LOC120070724 [Benincasa hispida] >XP_038878507.1 uncharacterized protein LOC120070724 [Benincasa hispida] >XP_038878508.1 uncharacterized protein LOC120070724 [Benincasa hispida] >XP_038878509.1 uncharacterized protein LOC120070724 [Benincasa hispida] >XP_038878510.1 uncharacterized protein LOC120070724 [Benincasa hispida] >XP_038878511.1 uncharacterized protein LOC120070724 [Benincasa hispida])

HSP 1 Score: 413.7 bits (1062), Expect = 1.1e-111
Identity = 201/227 (88.55%), Postives = 216/227 (95.15%), Query Frame = 0


Query: 14  AAPTQVAFGSGGTSSTIRSFGLSKAGNRPRNEDGTLPSSASKEYVEPWDYYSYYPVTLPL 73
           AAPTQVAFGSGGTSST+RS+G+SKAGNRPRNEDGTLPSS SKEYVEPWDYYSYYPVTLPL
Sbjct: 67  AAPTQVAFGSGGTSSTLRSYGVSKAGNRPRNEDGTLPSSTSKEYVEPWDYYSYYPVTLPL 126

Query: 74  RRPYSGNPDSLNEEEFGEASETITYDENTTTPALDLGLMEDNPETDIFFLQLPPMVPVIK 133
           RRPYSGNPDSLNEEEFGEASE   YDENTTTPA+DLGL+E+NPE D+FFLQLPPMVP++K
Sbjct: 127 RRPYSGNPDSLNEEEFGEASENFAYDENTTTPAMDLGLLEENPEMDVFFLQLPPMVPMVK 186

Query: 134 QSSTLEGMEAANSLEQRKGSQPRQKTCSLNELPSGSIGKLLVYRSGAVKLKLGDIVYDVS 193
           QSST+EG  AANSLEQ KGSQPRQK C +NELPSGS+GKLLVYRSGAVKLKLGDI+YDVS
Sbjct: 187 QSSTVEGTGAANSLEQSKGSQPRQKACLMNELPSGSMGKLLVYRSGAVKLKLGDIIYDVS 246

Query: 194 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKNIEDL 241
           SGMDCGFAQDVAA+NVEGK CCIVGELSKRAILTPDVDS+LKNIEDL
Sbjct: 247 SGMDCGFAQDVAAVNVEGKCCCIVGELSKRAILTPDVDSVLKNIEDL 293

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BLAST of Tan0022060 vs. NCBI nr
Match: XP_004144123.1 (DNA-directed RNA polymerase III subunit RPC4 [Cucumis sativus] >XP_031736467.1 DNA-directed RNA polymerase III subunit RPC4 [Cucumis sativus] >XP_031736470.1 DNA-directed RNA polymerase III subunit RPC4 [Cucumis sativus] >KGN66283.1 hypothetical protein Csa_007538 [Cucumis sativus])

HSP 1 Score: 405.2 bits (1040), Expect = 3.9e-109
Identity = 197/227 (86.78%), Postives = 215/227 (94.71%), Query Frame = 0


Query: 14  AAPTQVAFGSGGTSSTIRSFGLSKAGNRPRNEDGTLPSSASKEYVEPWDYYSYYPVTLPL 73
           AAPTQVAFGSGG+SST+RS+G+SKAGNRPRNEDGTLP+S SKEYVEPWDYYSYYPVTLPL
Sbjct: 67  AAPTQVAFGSGGSSSTLRSYGVSKAGNRPRNEDGTLPASTSKEYVEPWDYYSYYPVTLPL 126

Query: 74  RRPYSGNPDSLNEEEFGEASETITYDENTTTPALDLGLMEDNPETDIFFLQLPPMVPVIK 133
           RRPYSGNPDSLNEEEFGEASE +TYDENTTT A++LGL+E+NPE D+ FLQLPPMVP+IK
Sbjct: 127 RRPYSGNPDSLNEEEFGEASENLTYDENTTTAAMNLGLLEENPEADVLFLQLPPMVPMIK 186

Query: 134 QSSTLEGMEAANSLEQRKGSQPRQKTCSLNELPSGSIGKLLVYRSGAVKLKLGDIVYDVS 193
           QSS++E M + NS EQ K SQPRQKTCS+NELPSGSIGKLLVYRSGAVKLKLGDI+YDVS
Sbjct: 187 QSSSVEDMGSGNSSEQNKASQPRQKTCSMNELPSGSIGKLLVYRSGAVKLKLGDIIYDVS 246

Query: 194 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKNIEDL 241
           SGMDCGFAQ+VAAINVEGK CCIVGELSKRAILTPDVDSMLKNIEDL
Sbjct: 247 SGMDCGFAQEVAAINVEGKRCCIVGELSKRAILTPDVDSMLKNIEDL 293

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BLAST of Tan0022060 vs. NCBI nr
Match: XP_022147557.1 (DNA-directed RNA polymerase III subunit rpc4 [Momordica charantia])

HSP 1 Score: 403.7 bits (1036), Expect = 1.1e-108
Identity = 199/227 (87.67%), Postives = 215/227 (94.71%), Query Frame = 0


Query: 14  AAPTQVAFGSGGTSSTIRSFGLSKAGNRPRNEDGTLPSSASKEYVEPWDYYSYYPVTLPL 73
           AAPTQVAFGSGGTS+TIRS+G+ KAGNRPRNEDGTLPSS SKEYVEPWDYYSYYPVTLPL
Sbjct: 67  AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPL 126

Query: 74  RRPYSGNPDSLNEEEFGEASETITYDENTTTPALDLGLMEDNPETDIFFLQLPPMVPVIK 133
           RRPYSG+PDSLNEEEFGEASE  TYDE+ TTPA+DLGL+E+NPET++FFLQ+PPMVPV K
Sbjct: 127 RRPYSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTK 186

Query: 134 QSSTLEGMEAANSLEQRKGSQPRQKTCSLNELPSGSIGKLLVYRSGAVKLKLGDIVYDVS 193
           QSST+EGM AA+S EQ KGS+ RQ  CSLNELPSGS+GKLLVYRSGAVKLKLGDIVYDVS
Sbjct: 187 QSSTVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVS 246

Query: 194 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKNIEDL 241
           SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLK+IEDL
Sbjct: 247 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIEDL 293

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BLAST of Tan0022060 vs. NCBI nr
Match: XP_008450913.1 (PREDICTED: DNA-directed RNA polymerase III subunit RPC4 [Cucumis melo])

HSP 1 Score: 402.9 bits (1034), Expect = 1.9e-108
Identity = 195/227 (85.90%), Postives = 214/227 (94.27%), Query Frame = 0


Query: 14  AAPTQVAFGSGGTSSTIRSFGLSKAGNRPRNEDGTLPSSASKEYVEPWDYYSYYPVTLPL 73
           AAPTQVAFGSGG+SST+RS+G+SKAGNRPRNEDGTLP+S SKEYVEPWDYYSYYPVTLPL
Sbjct: 67  AAPTQVAFGSGGSSSTLRSYGVSKAGNRPRNEDGTLPASTSKEYVEPWDYYSYYPVTLPL 126

Query: 74  RRPYSGNPDSLNEEEFGEASETITYDENTTTPALDLGLMEDNPETDIFFLQLPPMVPVIK 133
           RRPYSGNPDSLNEEEFGEASE  TYDENTTT A++LGL+E+NPETD+FFLQLPPMVP+IK
Sbjct: 127 RRPYSGNPDSLNEEEFGEASENFTYDENTTTAAMNLGLLEENPETDVFFLQLPPMVPMIK 186

Query: 134 QSSTLEGMEAANSLEQRKGSQPRQKTCSLNELPSGSIGKLLVYRSGAVKLKLGDIVYDVS 193
           QSST+E M + N  EQ   SQ RQKTCS+NELPSGS+GKLLVYRSGAVKLKLGDI+YDVS
Sbjct: 187 QSSTVENMGSGNVSEQNSASQTRQKTCSMNELPSGSMGKLLVYRSGAVKLKLGDIIYDVS 246

Query: 194 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKNIEDL 241
           SGMDCGFAQ+VAAIN+EGKHCCIVGELSKRAILTPDVDSML+NIEDL
Sbjct: 247 SGMDCGFAQEVAAINMEGKHCCIVGELSKRAILTPDVDSMLRNIEDL 293

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BLAST of Tan0022060 vs. NCBI nr
Match: KAG6588073.1 (DNA-directed RNA polymerase III subunit RPC4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 399.1 bits (1024), Expect = 2.8e-107
Identity = 198/227 (87.22%), Postives = 214/227 (94.27%), Query Frame = 0


Query: 14  AAPTQVAFGSGGTSSTIRSFGLSKAGNRPRNEDGTLPSSASKEYVEPWDYYSYYPVTLPL 73
           +APTQVAFG+GGTSS++R++G SKAGN+ RNEDGTLPS  SKEYVEPWDYYSYYPVTLPL
Sbjct: 55  SAPTQVAFGTGGTSSSLRTYGFSKAGNKLRNEDGTLPS--SKEYVEPWDYYSYYPVTLPL 114

Query: 74  RRPYSGNPDSLNEEEFGEASETITYDENTTTPALDLGLMEDNPETDIFFLQLPPMVPVIK 133
           RRPYSGNPDSLNE EFGEASE  TYDENTTTPA+DLGL+EDNPET+IFFLQLPPMVPV +
Sbjct: 115 RRPYSGNPDSLNEYEFGEASEARTYDENTTTPAMDLGLLEDNPETNIFFLQLPPMVPVTE 174

Query: 134 QSSTLEGMEAANSLEQRKGSQPRQKTCSLNELPSGSIGKLLVYRSGAVKLKLGDIVYDVS 193
           QSST EGMEAANSLEQ KGSQP QKTCSLNE PSGS+GKLLVYRSGAVKLKLG+IVYDVS
Sbjct: 175 QSSTAEGMEAANSLEQNKGSQPPQKTCSLNEFPSGSMGKLLVYRSGAVKLKLGNIVYDVS 234

Query: 194 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKNIEDL 241
           SGMDCGFAQ++AAINVEGK CCIVGELSKRA+LTPDVDSMLKNIEDL
Sbjct: 235 SGMDCGFAQELAAINVEGKRCCIVGELSKRAVLTPDVDSMLKNIEDL 279

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BLAST of Tan0022060 vs. ExPASy TrEMBL
Match: A0A0A0LWX7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G595870 PE=4 SV=1)

HSP 1 Score: 405.2 bits (1040), Expect = 1.9e-109
Identity = 197/227 (86.78%), Postives = 215/227 (94.71%), Query Frame = 0


Query: 14  AAPTQVAFGSGGTSSTIRSFGLSKAGNRPRNEDGTLPSSASKEYVEPWDYYSYYPVTLPL 73
           AAPTQVAFGSGG+SST+RS+G+SKAGNRPRNEDGTLP+S SKEYVEPWDYYSYYPVTLPL
Sbjct: 67  AAPTQVAFGSGGSSSTLRSYGVSKAGNRPRNEDGTLPASTSKEYVEPWDYYSYYPVTLPL 126

Query: 74  RRPYSGNPDSLNEEEFGEASETITYDENTTTPALDLGLMEDNPETDIFFLQLPPMVPVIK 133
           RRPYSGNPDSLNEEEFGEASE +TYDENTTT A++LGL+E+NPE D+ FLQLPPMVP+IK
Sbjct: 127 RRPYSGNPDSLNEEEFGEASENLTYDENTTTAAMNLGLLEENPEADVLFLQLPPMVPMIK 186

Query: 134 QSSTLEGMEAANSLEQRKGSQPRQKTCSLNELPSGSIGKLLVYRSGAVKLKLGDIVYDVS 193
           QSS++E M + NS EQ K SQPRQKTCS+NELPSGSIGKLLVYRSGAVKLKLGDI+YDVS
Sbjct: 187 QSSSVEDMGSGNSSEQNKASQPRQKTCSMNELPSGSIGKLLVYRSGAVKLKLGDIIYDVS 246

Query: 194 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKNIEDL 241
           SGMDCGFAQ+VAAINVEGK CCIVGELSKRAILTPDVDSMLKNIEDL
Sbjct: 247 SGMDCGFAQEVAAINVEGKRCCIVGELSKRAILTPDVDSMLKNIEDL 293

[Close]

BLAST of Tan0022060 vs. ExPASy TrEMBL
Match: A0A6J1D2P1 (DNA-directed RNA polymerase III subunit rpc4 OS=Momordica charantia OX=3673 GN=LOC111016454 PE=4 SV=1)

HSP 1 Score: 403.7 bits (1036), Expect = 5.5e-109
Identity = 199/227 (87.67%), Postives = 215/227 (94.71%), Query Frame = 0


Query: 14  AAPTQVAFGSGGTSSTIRSFGLSKAGNRPRNEDGTLPSSASKEYVEPWDYYSYYPVTLPL 73
           AAPTQVAFGSGGTS+TIRS+G+ KAGNRPRNEDGTLPSS SKEYVEPWDYYSYYPVTLPL
Sbjct: 67  AAPTQVAFGSGGTSATIRSYGIPKAGNRPRNEDGTLPSSISKEYVEPWDYYSYYPVTLPL 126

Query: 74  RRPYSGNPDSLNEEEFGEASETITYDENTTTPALDLGLMEDNPETDIFFLQLPPMVPVIK 133
           RRPYSG+PDSLNEEEFGEASE  TYDE+ TTPA+DLGL+E+NPET++FFLQ+PPMVPV K
Sbjct: 127 RRPYSGDPDSLNEEEFGEASENRTYDESMTTPAMDLGLLEENPETNVFFLQIPPMVPVTK 186

Query: 134 QSSTLEGMEAANSLEQRKGSQPRQKTCSLNELPSGSIGKLLVYRSGAVKLKLGDIVYDVS 193
           QSST+EGM AA+S EQ KGS+ RQ  CSLNELPSGS+GKLLVYRSGAVKLKLGDIVYDVS
Sbjct: 187 QSSTVEGMVAASSSEQSKGSRTRQNFCSLNELPSGSMGKLLVYRSGAVKLKLGDIVYDVS 246

Query: 194 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKNIEDL 241
           SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLK+IEDL
Sbjct: 247 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKDIEDL 293

[Close]

BLAST of Tan0022060 vs. ExPASy TrEMBL
Match: A0A1S3BPR1 (DNA-directed RNA polymerase III subunit RPC4 OS=Cucumis melo OX=3656 GN=LOC103492360 PE=4 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 9.3e-109
Identity = 195/227 (85.90%), Postives = 214/227 (94.27%), Query Frame = 0


Query: 14  AAPTQVAFGSGGTSSTIRSFGLSKAGNRPRNEDGTLPSSASKEYVEPWDYYSYYPVTLPL 73
           AAPTQVAFGSGG+SST+RS+G+SKAGNRPRNEDGTLP+S SKEYVEPWDYYSYYPVTLPL
Sbjct: 67  AAPTQVAFGSGGSSSTLRSYGVSKAGNRPRNEDGTLPASTSKEYVEPWDYYSYYPVTLPL 126

Query: 74  RRPYSGNPDSLNEEEFGEASETITYDENTTTPALDLGLMEDNPETDIFFLQLPPMVPVIK 133
           RRPYSGNPDSLNEEEFGEASE  TYDENTTT A++LGL+E+NPETD+FFLQLPPMVP+IK
Sbjct: 127 RRPYSGNPDSLNEEEFGEASENFTYDENTTTAAMNLGLLEENPETDVFFLQLPPMVPMIK 186

Query: 134 QSSTLEGMEAANSLEQRKGSQPRQKTCSLNELPSGSIGKLLVYRSGAVKLKLGDIVYDVS 193
           QSST+E M + N  EQ   SQ RQKTCS+NELPSGS+GKLLVYRSGAVKLKLGDI+YDVS
Sbjct: 187 QSSTVENMGSGNVSEQNSASQTRQKTCSMNELPSGSMGKLLVYRSGAVKLKLGDIIYDVS 246

Query: 194 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKNIEDL 241
           SGMDCGFAQ+VAAIN+EGKHCCIVGELSKRAILTPDVDSML+NIEDL
Sbjct: 247 SGMDCGFAQEVAAINMEGKHCCIVGELSKRAILTPDVDSMLRNIEDL 293

[Close]

BLAST of Tan0022060 vs. ExPASy TrEMBL
Match: A0A6J1HTZ8 (uncharacterized protein LOC111467450 OS=Cucurbita maxima OX=3661 GN=LOC111467450 PE=4 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 3.0e-107
Identity = 198/227 (87.22%), Postives = 213/227 (93.83%), Query Frame = 0


Query: 14  AAPTQVAFGSGGTSSTIRSFGLSKAGNRPRNEDGTLPSSASKEYVEPWDYYSYYPVTLPL 73
           AAPTQVAFG+GGTSS++R+FG SKAGN+ RNEDGTLP   S+EYVEPWDYYSYYPVTLPL
Sbjct: 67  AAPTQVAFGTGGTSSSLRTFGFSKAGNKLRNEDGTLP---SREYVEPWDYYSYYPVTLPL 126

Query: 74  RRPYSGNPDSLNEEEFGEASETITYDENTTTPALDLGLMEDNPETDIFFLQLPPMVPVIK 133
           RRPYSGNPDSLNE EFGEASE+ TYDENTTTPA+DLGL+EDNPET+IFFLQLPPMVPV +
Sbjct: 127 RRPYSGNPDSLNEYEFGEASESRTYDENTTTPAMDLGLLEDNPETNIFFLQLPPMVPVTE 186

Query: 134 QSSTLEGMEAANSLEQRKGSQPRQKTCSLNELPSGSIGKLLVYRSGAVKLKLGDIVYDVS 193
           QSST EGMEAANSLEQ K SQP QKTCSLNE PSGS+GKLLVYRSGAVKLKLGDIVYDVS
Sbjct: 187 QSSTAEGMEAANSLEQNKDSQPPQKTCSLNEFPSGSMGKLLVYRSGAVKLKLGDIVYDVS 246

Query: 194 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKNIEDL 241
           SGMDCGFAQ++AAINVEGK CCIVGELSKRA+LTPDVDSMLKNIEDL
Sbjct: 247 SGMDCGFAQELAAINVEGKRCCIVGELSKRAVLTPDVDSMLKNIEDL 290

[Close]

BLAST of Tan0022060 vs. ExPASy TrEMBL
Match: A0A6J1HBC9 (uncharacterized protein LOC111461827 OS=Cucurbita moschata OX=3662 GN=LOC111461827 PE=4 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 6.7e-107
Identity = 196/227 (86.34%), Postives = 213/227 (93.83%), Query Frame = 0


Query: 14  AAPTQVAFGSGGTSSTIRSFGLSKAGNRPRNEDGTLPSSASKEYVEPWDYYSYYPVTLPL 73
           AAPTQVAFGSGGTSSTIRSFG+SKAGNR  NEDG+LPS+ SKEYVEPWDYYSYYPVTLP+
Sbjct: 67  AAPTQVAFGSGGTSSTIRSFGISKAGNRLGNEDGSLPSTTSKEYVEPWDYYSYYPVTLPM 126

Query: 74  RRPYSGNPDSLNEEEFGEASETITYDENTTTPALDLGLMEDNPETDIFFLQLPPMVPVIK 133
           R PYSGNPDSLNEEEFGE SET++YDE TTTPA+DLGL+E+NPETDIFFLQLPPMVPVIK
Sbjct: 127 RSPYSGNPDSLNEEEFGEGSETLSYDERTTTPAVDLGLLEENPETDIFFLQLPPMVPVIK 186

Query: 134 QSSTLEGMEAANSLEQRKGSQPRQKTCSLNELPSGSIGKLLVYRSGAVKLKLGDIVYDVS 193
           QSST+EG+ AANS E    SQPRQKTCS+NELPSGS+GKLLVYRSGAVKLKLGDI+YDVS
Sbjct: 187 QSSTVEGIGAANSSE----SQPRQKTCSMNELPSGSMGKLLVYRSGAVKLKLGDIIYDVS 246

Query: 194 SGMDCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKNIEDL 241
           SGM CGFAQDVAAINVEGK+CC +GELSKRAILTPDV+SMLKNIEDL
Sbjct: 247 SGMQCGFAQDVAAINVEGKYCCFIGELSKRAILTPDVESMLKNIEDL 289

[Close]

BLAST of Tan0022060 vs. TAIR 10
Match: AT5G09380.1 (RNA polymerase III RPC4 )

HSP 1 Score: 184.5 bits (467), Expect = 1.0e-46
Identity = 105/224 (46.88%), Postives = 152/224 (67.86%), Query Frame = 0


Query: 17  TQVAFGSGGTSSTIRSFGLSKAGNRPRNEDGTLPSSASKEYVEPWDYYSYYPVTLPLRRP 76
           +QVA+  GG  ++ RS   +K  NR     G+  S+   EY EPWDYYSYYP+TLP+RRP
Sbjct: 64  SQVAW-LGGVVNSTRS---NKYLNRSNGAYGS-TSTQEIEYKEPWDYYSYYPITLPMRRP 123

Query: 77  YSGNPDSLNEEEFGEASETITYDENTTTPALDLGLMEDNPETDIFFLQLPPMVPVIKQSS 136
           Y+G+P+ L+ EEF +A     + E++   A +LGLMED+ E  +FF++L P VP+   S+
Sbjct: 124 YAGDPEVLDVEEFMQAG---GHHEDSLNTAANLGLMEDSGEQKMFFMRL-PSVPL--AST 183

Query: 137 TLEGMEAANSLEQRKGSQPRQKTCSLNELPSGSIGKLLVYRSGAVKLKLGDIVYDVSSGM 196
             E +E   +++        +KT  L  LP G +GKLLVY+SGAVK+KLG+++YDVS G+
Sbjct: 184 PTENLETRPNIK----GPVEKKTVDLKALPEGYMGKLLVYKSGAVKMKLGEVLYDVSPGL 243

Query: 197 DCGFAQDVAAINVEGKHCCIVGELSKRAILTPDVDSMLKNIEDL 241
              FAQDV  +N E K+CC+VG++ K A+LTPD+DS+LK+IE++
Sbjct: 244 KSEFAQDVMVVNTEQKNCCLVGDVYKHAVLTPDIDSILKDIENI 272

[Close]

BLAST of Tan0022060 vs. TAIR 10
Match: AT4G25180.1 (RNA polymerase III RPC4 )

HSP 1 Score: 146.7 bits (369), Expect = 2.3e-35
Identity = 93/256 (36.33%), Postives = 138/256 (53.91%), Query Frame = 0


Query: 14  AAPTQVAFGSGGTSSTIRSFGLSKAGNRPRN---------------------EDG----T 73
           A+  +VAF    +   IRSFG+ K  ++P +                     EDG     
Sbjct: 61  ASSPEVAFQPSLSPLAIRSFGVPKEDDKPNSDVNPSSPASILPAVSSVTAAQEDGEEVHN 120

Query: 74  LPSSASKEYVEPWDY-YSYYPVTLPLRRPYSGNPDSLNEEEFGEASETITYDENTTTPAL 133
             +    +YVEPWDY  SYYP  LPLR+P SG+ + L++EEFGE ++   YDENT   A 
Sbjct: 121 FVTRTGDDYVEPWDYRNSYYPTVLPLRKPNSGDIELLDQEEFGEVAKNRDYDENTINSAE 180

Query: 134 DLGLME-DNPETDIFFLQLPPMVPVIKQSSTLEGMEAANSLEQRKG--SQPRQKTCSLNE 193
           +LGL    + +  +F  ++P  +PV+KQ++   G     S+ +     S P         
Sbjct: 181 ELGLTSVQHSKKQMFIFKIPDCLPVVKQTT---GATTKRSVREYSSGISNP------FEG 240

Query: 194 LPSGSIGKLLVYRSGAVKLKLGDIVYDVSSGMDCGFAQDVAAINVEGKHCCIVGELSKRA 241
           LP G +GK+LVY+SGAVKLK+GD ++DVS G       DV AI+++G++C  +G  +K  
Sbjct: 241 LPEGFMGKMLVYKSGAVKLKVGDALFDVSPGPGTKIPNDVVAIDIKGRNCSRIGSSAKFV 300

The following BLAST results are available for this feature:

BLAST of Tan0022060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5E9Z7	9.6e-10	24.52	DNA-directed RNA polymerase III subunit RPC4 OS=Bos taurus OX=9913 GN=POLR3D PE=...	[more]
Q91WD1	1.3e-09	25.48	DNA-directed RNA polymerase III subunit RPC4 OS=Mus musculus OX=10090 GN=Polr3d ...	[more]
P05423	1.1e-08	25.95	DNA-directed RNA polymerase III subunit RPC4 OS=Homo sapiens OX=9606 GN=POLR3D P...	[more]
O74857	2.9e-06	26.01	DNA-directed RNA polymerase III subunit rpc4 OS=Schizosaccharomyces pombe (strai...	[more]
P25441	3.8e-06	27.34	DNA-directed RNA polymerase III subunit RPC4 OS=Saccharomyces cerevisiae (strain...	[more]

BLAST of Tan0022060 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878504.1	1.1e-111	88.55	uncharacterized protein LOC120070724 [Benincasa hispida] >XP_038878505.1 unchara...	[more]
XP_004144123.1	3.9e-109	86.78	DNA-directed RNA polymerase III subunit RPC4 [Cucumis sativus] >XP_031736467.1 D...	[more]
XP_022147557.1	1.1e-108	87.67	DNA-directed RNA polymerase III subunit rpc4 [Momordica charantia]	[more]
XP_008450913.1	1.9e-108	85.90	PREDICTED: DNA-directed RNA polymerase III subunit RPC4 [Cucumis melo]	[more]
KAG6588073.1	2.8e-107	87.22	DNA-directed RNA polymerase III subunit RPC4, partial [Cucurbita argyrosperma su...	[more]

BLAST of Tan0022060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LWX7	1.9e-109	86.78	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G595870 PE=4 SV=1	[more]
A0A6J1D2P1	5.5e-109	87.67	DNA-directed RNA polymerase III subunit rpc4 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A1S3BPR1	9.3e-109	85.90	DNA-directed RNA polymerase III subunit RPC4 OS=Cucumis melo OX=3656 GN=LOC10349...	[more]
A0A6J1HTZ8	3.0e-107	87.22	uncharacterized protein LOC111467450 OS=Cucurbita maxima OX=3661 GN=LOC111467450...	[more]
A0A6J1HBC9	6.7e-107	86.34	uncharacterized protein LOC111461827 OS=Cucurbita moschata OX=3662 GN=LOC1114618...	[more]

BLAST of Tan0022060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G09380.1	1.0e-46	46.88	RNA polymerase III RPC4	[more]
AT4G25180.1	2.3e-35	36.33	RNA polymerase III RPC4	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007811	DNA-directed RNA polymerase III subunit RPC4	PFAM	PF05132	RNA_pol_Rpc4	coord: 134..193 e-value: 6.2E-12 score: 46.2
IPR007811	DNA-directed RNA polymerase III subunit RPC4	PANTHER	PTHR13408	DNA-DIRECTED RNA POLYMERASE III	coord: 53..193
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 33..52
None	No IPR available	PANTHER	PTHR13408:SF6	DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC4-LIKE ISOFORM X1	coord: 53..193

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0022060.1	Tan0022060.1	mRNA
Tan0022060.2	Tan0022060.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006383	transcription by RNA polymerase III
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity