Tan0022016 (gene) Snake gourd v1

Overview


Name	Tan0022016
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	calmodulin-like
Location	LG02: 86299612 .. 86305765 (-)
RNA-Seq Expression	Tan0022016
Synteny	Tan0022016

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCACTCAATCGATGGTTGGAGGCGTCGAACAACGGACGATCGAGCATCCGATTTGACGGAGAAACAAATGGTTGCTGTGAGGTCGGCAAACACCCATTCACGCCAGCAAATGACGACTGCTCGGCGACAACTAAACGACGCCAAGATTCGCGAAGGGTTGCAGTGGTGCGATTTTTTGATAGAGCTTCACGCACTTGGCGGCTCAACTTCTAGCGAGCGACGTTCGGCTGGATTTTGGGTGTGGCAATGCATAGTGGCGACTCCTGGCTGGCAGTTCCTCGTCGCTGGTAACTATAGCTTCTCTCCAATGGGGGCTCGTGCGAAGAGGGAGAAGGGAGCGACTTCGTTTTTCGTGCACACTAGAGGACAAAAATTAGGTCTATTTGGAAACCGTTGATCAGCACCCATTTACTCTTTTACCCCTCTTTTCATTTAATATATAATCAAGCCACCAAACCCACTTTCCAAACATTAAGGGATTGTTTGGGGCGCATGTTCGGAATCAAAATCCTGGGAATCAAAAGCCTGAAAGGTTTGTCCTGAGGGGTTCTGAAGCCTAGAAATCAGAAAACTGTGTTTGGGTTACAGTGTTCTGAAGTCTGGGAATCAGAAAACTATGTTTGGGGTGCAGTGTTCTGAAGCCTGGGAATCAGAAAGCTGTGTCTTGGGTGCAGTTTTCTAACCTCTAGGTTTGGTACAGTTTTGTTAGACATTAGCTTCTACAATTCACAGAAGATCAATTACAAAAATTTGAAACGAATAAAATAGTTAAAAGAAAAAAAGGTGAAACAAAACTGTACCAAACCTAGAGTTCAGAAAACTGCACACAAGACACAGCTTTTTAATTCTCAGGCTTCAGAACACTACACCCCAAACACAGTTTTTTGATTCCCAAACTTTAGAACACTGCACCCCAAACACAGTTTTCTGATTCCCAGGCTTCAGAACCCCTCAGGACAAACCCTCCAGGCTTCTGATTCCCGGGATTCTGATTCTGAACTTGCACCCCAAACAACCCCTAAATCATCACAGATAAAATGTCAAATTCCAATTAGTTTTCCTTTTTTTTCTCTCTCTTTAAGCTAAATTTAATTTAAGCAGTAGTATTTTAATTTCTCACCTAGTCATTTTCCAAGAAATATTTTAATTTAAAAGGTGAAATTAACCATATCTGAAATTCTCAGGCATTACAAAAATAATCTTCGGTAAAATTATAAGGGCATAACAATAAAATGTTATACTTTCTTTCATTCCTTTTCGTTCTTAGGCCCAATCTCTCTTCCTTTTAGGATGCATCTTAGATATTTGTTGAACCTTTCGGTTATTTTAAAACCTTGGCTTAGATGTTGTTTTGTTACCTTTCGAACTTTATCATGTTGTAGGTTCTGCTCTAGAGAACATTTCAATCTAAATTCGTTTGCATTACTTTATGTGTCTGTTGGAATTCTGAATTATGAGCCCAACCTAATCTCTGATTGAAGTTTGGTGGAGGTTCGACATTTGTTGTGTCTTAAGGAGTGTTTGTTTTGATTTACAAGTCACAGAAAGTTTTTGGTTTTCTCCTAGTTTAGCCGTTATGTTACAGAAATTTTGGCACAAGCAGCGTGGTTGGCTAACCTCCCTAACTAGGTTCGTTCCAAAGTTTAGGATCGTTATACTTTTTCTCTCTATTTTTTAAAATTTTTACCTCTTGCTCTATTTTTTAGTTCTTATACTTTTTTGTCCCTAACTCTTAACTGTTTTTTCCTCAAAGTTTTCTTCCTAAAGTTGTTACGGCCCAGTCCTTTCTCGTTTTAAAAAAAAAAAAAATTACAGAAAGTCGAGGGGGCGAACGACTCGCCCCCTCCTTCCTCCATCCGCTGTGAGTGTGTAGGCGAAACGGCCGCCGGCCGTTTCGGCCGCCGGCCACGCCCTCTTCCCCTCTCTTTTTCTCCTCTCTCCCTCGTCTCCCTCTCTCACGAGCCTCTCTCATTCTCTCGGTCTCTCTCTCTCGCCCCCCTCTCCCTCTCCTCACCGTCTCTCTCTCTCTCTCTCCTCTCCGATCCCTTCTCTCACTCTATCTCTCTCACGCAACTCTCTCCCCTCAGAGTGCAGTCTCCGCCGCCGCCACGCCACGCAGACGCGCTTAGCCGCCTGGCCGAGAACCGCCGCAATGCCAGCTGCGATCAAGCTGCCGGCGTGCCGAGATCCGCGAGACCCACTGCTTTGGTTCACCGGCTCGAGTCCTTTTCGGCGGTTTTACGCGGCGGAGTCCGGTTCTTGATAGATCGGGTCGCGAGGATTCCAGATCTGCCACCCACGCGCCACCACTATCACCCACGCGCCGCCGTCCGCCGGTACACGAGCCGCCGTCCGCCGCCACGCGCGCGCGCCGCGCCGATTCCACGCGCGCGCCGCTGCGAGCGCGACCGTCCTCTTCCGCCCGGGCCGCCTCGGTTCGTTCATAAGTCCGCTTTGGCACTCGTGTGTGTGAGTTCGCTCCTCTTAATTAGTTTTCCTTGCGTTAGATTCATCGTGTTTTAGTTCATTAATTTGTGTGTTTTCGTTAGGAGCCTTGAAAGTAGAAATGCGTTTGGAGCACGCCACGATACTCAAAGTCGCTCGTTGTCGCTTTGGCTTTCAAGTAAGTAGCCGTTTGACTAAACCACCCCTAAGTGTAGGGCTTAGGAGCTTCACGCATGCTAAAATTGTTTCAAAGTGCTTAATGTTTTAGAGCGTGTAACAACAAACTGGACTTGTAGTAGCATGCTAGGTTGTGGTAAACGCTCTGCGATACTTGATTTACGTGATGCTTGTACATTTCGTGATGCTTGTACTTTTGTTGCGAATATAATGAATCGGTGTTGTAGTTGTTATTCTTTCGTGCTAAAAGGATTGCCTTCCGTGTTTAGTTGTGATCTTGTTAGAAAAGTTAACCTAAGCTTGTGTTGAGAATGATACTTATGTTAGAGGTACATGTCGTGCCGAACTTGTTGAATATGCTAGAATTGTATCTTGTGTGTCCCAAGCCTAGTCGTCCCGTGCTTAGTCCAGTAGTGACTCGGGAAGTGCCGCTGAGCCTTAGGATTGTAGTTAGCACAACTAGCCCTAGTGCAGCCCCAAATCCTAAGTATCCATGTAACCCTTAGCAGCGCATCGAGACTCGAGGTTGGCGAGATGATGTATGGGATGTGATGGTCCGCCCTCCATCCAGGTGTAGCGACTCGGGATACGATCCTTAGAGTCGAGAGGTTGGATAGAGTCCTTCGCCTCTAGTACAGCCCGGAATGAGCCCGAGGTCGATTGTGCGCTGTAGTACCGCTTTGATACTTATGGGATGTGGTGGTCCGCCCCATCCGGTTGTGCGACTTCGGGGTGTGATCCTTAGAGTCAAAGGGGTTGGATAGAGTCCTTCGCCCGGGCCCGGAATGAGTCTCGTGGCCACGTGCTGCCAACCAAGTCTAGGAGCTAAGGGTAGGACAGAACGTAACTAAGAAAAGTGAGCAAGAGTAGGGCCAGGATACGTGTGGAGGTTGTTTGGCGGGCTAGGTATCGTGTTAGCTAAGTCTAGGGTCGTTCTTATGTTCTTTATTCTTGGACGAAGTGGGCTGCTTACTAAGTATTTCTATACTCACCCCTTCTTTATTTTCTTTTCGTTGCAGGTAAGGCAAACCAGTGAGATGACCACCGACCAGCCAGAGACCTCACTGAGAGGCTATGGAAGACTTGTAGGATTTAGGGGTTGCGTTAGGAGTAGTTCTTTCGTTTTCTTTCCTTCCGTTGCGTTTTAACTATTTGTTATGTTAATTTTAGATTGTATCGTTCCTGTTTCTTTCGTATTCAAAGCATGTTCTGCTGTTGAGCTTTCTGTTAACTCCGAGGGACTGGAGCCCAGCCTATTGTTGTTCTGTGTAGTTTGGTGGAGGCTGGGTACCCTCGTGAGTCTTTGAAGTGTTCTCGATTAGGTTCAGGCACTTGTAAAATTTTCGGTTTGTCCAGGTTGCGCCGTTATGCTGCCGGATTTTCGGCACGAACGGTACAACTTGGAAGTAATGCCTCGAGTTGTTGTGTTCAATTTTTTTTTTGGGGTGTTACAAAAGTTGTATCATTCTGCCCAAATTTATTAATTTATCTCTCTGTCTTTATTTCTCTATTTTTAAATTTCACTCTTTTTATTAAAATTCTTCTTCTAAATTTTTTGTCCCTCCATATCTCTAATTTTTTTCTCTTTGGATTTTTTCTCTAACTTGATCTTTTCTCTCTCTTAATTTTTAGTTCTCTCGGTATAACTTTTGTCGATCACCTTTTACTTTTTTTTAATCTCAAATTCAATACTTTTTTTAGTAACTTTGATGATTCTAAAATCACATTTGAAAACAAATATTAATAATTTTTTAAAAAAGATACTTTTAATTAAATTTAAATTACTTTTACAAGTGTTGAATAAATATTAAAATAATTTATAACTAAATACTTTTAAAAAATTAATTATAATTAAACTACTTTGCTTAAAACACTTATTTTAAAGTTACTCCTAAACATACCGTTTGAAAATCACTTAATTGTATGAAAAATACTATTAAAAGTATAGAATCTAACATTAAAATAATTATATAAAAACCACTTTTAATAAATTAATTATAAATTAATTAATTATACTAAAATTAATATTTCTAAAATTATTCTCAAACATGAATGTCAACCACGTTTTGTGGGGCTCATCATGAATCTAATTCCTCCCCTCTGCAAAAAGGGGAAAAAAGTTTTGATTGATGCTTTTATTATTATTTTTCTTATCAATAGATTTCTACCGACAACGTAAGGTTATCACCTATTGCATCCTTTTGCGAGAATTATATTTCTCCCACTAAAATTGTTGATTGACAACTAGAAAGGTAAGCTAATTCATATCAAAACTTGTTAAGATTAGTTCGAATTCACATGGTGATACCCAATCAAAAGTAAATTTGTAAACAAATTAAGAAAAACGACAAAGACTCTATAGGAAGAAAAAAAGTTGGAATTATTTCATCTTAACAATTCTATAAAGCAACACACTGATGCTTAATTATCACAAAAAGAGTGTTTATTCTCATGTTTGTAATTTCCTTACTTCTTGGAAAAAAAACAAGCAATATTCTATCATATTATATTTGTTGTCTTTTAATATCTAATTTATTACAAACACGCTGATATAAATATATAATTTACGTACTTTTAACACTTTATGCTATTGTCAACCCTAAATATAGTTCAACTAGTTAAAGAATATGTTTTCGACAAAAGATTAGGTTCGAGTTCTTTTACCTCACTTTGTTGAACTAAAAATAAAAAAAAAAGAAACTTTATGCTATTTGATGAGAATCTAACCTCAAGAAACAAGACATGTGTACACACTACACACAAAAATAAATAAGGTTCTTCACCTGATTGAATCACTATAAAGAGCCCATCTCCATCTAAGGAAGCAAACAAAGCCTTAGGTTTTTTTTTTTCTCTTTTTCTTCCTTTCAACAATGCCAACAAATTCTTGTCAGCCCTGCAAATTTTCCTTTCTTACTCAAGAACAAGTAGACGAGTTCTTCAAAAAGTATGACAAAAATGGTGATGGTATGATCACCATAGATGAGCTGACTGGAATTTTCCCACAAACTAAGAATCATGGATCTAAAGCCCTACCTCCTCAAAATAAGGTTAACTCAAAGGCTCAACAACAGCCAAAAGAAAAAGGAAAGGCCAAAGCCAATGAAAGCCATGCAAATGCACACAAAGATAAAGAGCCAAAAAAGAAGCCTGATGATGCGAAAGCCAATTATGAATCTCCTTTGCACAAAGTTAGCTCAAAGGCCAACGCAAAGCCAGCAAAGAAGATTCAAGACAAAGCCCAAAAAGCCCCAGCAATAGAGTTGCACAAGAAGGGATCAATTATGCGTCTAAGTCGAGAACACATGAAGGAAATTTTTAGAGAGCACGATGTCGACGGCGATGGTTTTCTCGACTATGGTGAGCTAACAATGGCGTTTCACTTCTTAGACCCAGTTCTTTCTTGTTACAAGGCGCGCAATGGTATAGCTCATGCCGATGCCAATGGAGATGGGCTCATCAGCGAGGATGAGCTCGACAAACTTATTGACTATGCTGAAGAACTGTACGGAGAGGACTTGGTTTACTAA

mRNA sequence

ATGCACTCAATCGATGGTTGGAGGCGTCGAACAACGGACGATCGAGCATCCGATTTGACGGAGAAACAAATGGTTGCTGTGAGGTCGGCAAACACCCATTCACGCCAGCAAATGACGACTGCTCGGCGACAACTAAACGACGCCAAGATTCGCGAAGGGTTGCAGTGGTGCGATTTTTTGATAGAGCTTCACGCACTTGGCGGCTCAACTTCTAGCGAGCGACGTTCGGCTGGATTTTGGGTGTGGCAATGCATAGTGGCGACTCCTGGCTGGCAGTTCCTCGTCGCTGAACAAGTAGACGAGTTCTTCAAAAAGTATGACAAAAATGGTGATGGTATGATCACCATAGATGAGCTGACTGGAATTTTCCCACAAACTAAGAATCATGGATCTAAAGCCCTACCTCCTCAAAATAAGGTTAACTCAAAGGCTCAACAACAGCCAAAAGAAAAAGGAAAGGCCAAAGCCAATGAAAGCCATGCAAATGCACACAAAGATAAAGAGCCAAAAAAGAAGCCTGATGATGCGAAAGCCAATTATGAATCTCCTTTGCACAAAGTTAGCTCAAAGGCCAACGCAAAGCCAGCAAAGAAGATTCAAGACAAAGCCCAAAAAGCCCCAGCAATAGAGTTGCACAAGAAGGGATCAATTATGCGTCTAAGTCGAGAACACATGAAGGAAATTTTTAGAGAGCACGATGTCGACGGCGATGGTTTTCTCGACTATGGTGAGCTAACAATGGCGTTTCACTTCTTAGACCCAGTTCTTTCTTGTTACAAGGCGCGCAATGGTATAGCTCATGCCGATGCCAATGGAGATGGGCTCATCAGCGAGGATGAGCTCGACAAACTTATTGACTATGCTGAAGAACTGTACGGAGAGGACTTGGTTTACTAA

Coding sequence (CDS)

ATGCACTCAATCGATGGTTGGAGGCGTCGAACAACGGACGATCGAGCATCCGATTTGACGGAGAAACAAATGGTTGCTGTGAGGTCGGCAAACACCCATTCACGCCAGCAAATGACGACTGCTCGGCGACAACTAAACGACGCCAAGATTCGCGAAGGGTTGCAGTGGTGCGATTTTTTGATAGAGCTTCACGCACTTGGCGGCTCAACTTCTAGCGAGCGACGTTCGGCTGGATTTTGGGTGTGGCAATGCATAGTGGCGACTCCTGGCTGGCAGTTCCTCGTCGCTGAACAAGTAGACGAGTTCTTCAAAAAGTATGACAAAAATGGTGATGGTATGATCACCATAGATGAGCTGACTGGAATTTTCCCACAAACTAAGAATCATGGATCTAAAGCCCTACCTCCTCAAAATAAGGTTAACTCAAAGGCTCAACAACAGCCAAAAGAAAAAGGAAAGGCCAAAGCCAATGAAAGCCATGCAAATGCACACAAAGATAAAGAGCCAAAAAAGAAGCCTGATGATGCGAAAGCCAATTATGAATCTCCTTTGCACAAAGTTAGCTCAAAGGCCAACGCAAAGCCAGCAAAGAAGATTCAAGACAAAGCCCAAAAAGCCCCAGCAATAGAGTTGCACAAGAAGGGATCAATTATGCGTCTAAGTCGAGAACACATGAAGGAAATTTTTAGAGAGCACGATGTCGACGGCGATGGTTTTCTCGACTATGGTGAGCTAACAATGGCGTTTCACTTCTTAGACCCAGTTCTTTCTTGTTACAAGGCGCGCAATGGTATAGCTCATGCCGATGCCAATGGAGATGGGCTCATCAGCGAGGATGAGCTCGACAAACTTATTGACTATGCTGAAGAACTGTACGGAGAGGACTTGGTTTACTAA

Protein sequence

MHSIDGWRRRTTDDRASDLTEKQMVAVRSANTHSRQQMTTARRQLNDAKIREGLQWCDFLIELHALGGSTSSERRSAGFWVWQCIVATPGWQFLVAEQVDEFFKKYDKNGDGMITIDELTGIFPQTKNHGSKALPPQNKVNSKAQQQPKEKGKAKANESHANAHKDKEPKKKPDDAKANYESPLHKVSSKANAKPAKKIQDKAQKAPAIELHKKGSIMRLSREHMKEIFREHDVDGDGFLDYGELTMAFHFLDPVLSCYKARNGIAHADANGDGLISEDELDKLIDYAEELYGEDLVY

Homology

[Close]

BLAST of Tan0022016 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 9.7e-04
Identity = 26/70 (37.14%), Postives = 35/70 (50.00%), Query Frame = 0


Query: 215 GSIMRLSREHMKEIFREHDVDGDGFLDYGELTMAFHFLDPVLSCYKARNGIAHADANGDG 274
           GS      E M+ +FR+ D +GDG +   EL   F  L    +  +    +A ADA+GDG
Sbjct: 31  GSPAPTPEEEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELARMMAEADADGDG 90

Query: 275 LISEDELDKL 285
            IS DE   L
Sbjct: 91  FISLDEFAAL 100

[Close]

BLAST of Tan0022016 vs. NCBI nr
Match: XP_023002688.1 (calcium-binding protein H-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 107.8 bits (268), Expect = 1.6e-19
Identity = 78/200 (39.00%), Postives = 101/200 (50.50%), Query Frame = 0


Query: 91  WQFLVAEQVDEFFKKYDKNGDGMITIDELTGIFPQTKNHGSKALPPQNKVNSKAQQQPKE 150
           +Q    ++ +   KKYD NGDGM+T +EL       K  GSK L        K       
Sbjct: 6   FQTWAEDEANRLMKKYDTNGDGMLTKEELQVFL--HKARGSKQLNNNQGFVFKI------ 65

Query: 151 KGKAKANESHANAHKDKEPKKKPDDAKANYESPLHKVSSKANAKPAKKIQDKAQKAPAIE 210
               KAN + A+ H                       SS ++ K  K++QDK +K+    
Sbjct: 66  ---PKANNNVAHEH-----------------------SSNSHMKHKKQVQDKGKKSSTKF 125

Query: 211 LHKKGSIMRLSREHMKEIFREHDVDGDGFLDYGELTMAFHFLDPVLSCYKARNGIAHADA 270
              + S +RLSRE MKEIF+ HD + DGFLD  E+T AF FL  +L   KA   +A+AD 
Sbjct: 126 FDGEMSKLRLSREQMKEIFKNHDSNKDGFLDIKEVTKAFGFLGSILPFNKAHYAMAYADT 171

Query: 271 NGDGLISEDELDKLIDYAEE 291
           N DGLISE ELDKLIDYA +
Sbjct: 186 NHDGLISEFELDKLIDYASK 171

[Close]

BLAST of Tan0022016 vs. NCBI nr
Match: XP_023002888.1 (calmodulin-like [Cucurbita maxima])

HSP 1 Score: 107.1 bits (266), Expect = 2.7e-19
Identity = 66/139 (47.48%), Postives = 87/139 (62.59%), Query Frame = 0


Query: 153 KAKANESHANAHK--DKEPKKKPDDAKANYESPLHKVSSKANAKPAKKIQDKAQKAPAIE 212
           +A+A E   N  K  D    K+   A  ++    +  S K  AK  K +  KAQK PA  
Sbjct: 17  EAEAQEFIQNYDKNADSSLSKEELSAAIHHNQAKNPGSHKPEAK-KKDVAPKAQKVPAKA 76

Query: 213 LHKKGSIMRLSREHMKEIFREHDVDGDGFLDYGELTMAFHFLDPVLSCYKARNGIAHADA 272
             +KG+ M+LSR  +KEIF EHD+DGDG+L   ELT AF F   ++  YKA  G+A+ADA
Sbjct: 77  AEEKGASMKLSRSQIKEIFMEHDIDGDGYLSVSELTKAFSFFGSIIPLYKAHYGLAYADA 136

Query: 273 NGDGLISEDELDKLIDYAE 290
           +GDGLISE+EL+KL+DYAE
Sbjct: 137 DGDGLISEEELEKLVDYAE 154

[Close]

BLAST of Tan0022016 vs. NCBI nr
Match: XP_022951879.1 (calmodulin-like [Cucurbita moschata] >XP_023537873.1 calmodulin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 107.1 bits (266), Expect = 2.7e-19
Identity = 63/138 (45.65%), Postives = 85/138 (61.59%), Query Frame = 0


Query: 153 KAKANESHANAHKDKEPKKKPDDAKANYESPLHKVSSKANAKPAKK-IQDKAQKAPAIEL 212
           +A+A E   N  K+ +     ++  A       K     N +  KK +  KAQK PA   
Sbjct: 17  EAEAQELMQNYDKNADSSLSKEELSAAIHHNQAKNPGSHNPEAKKKDVAPKAQKVPAKAA 76

Query: 213 HKKGSIMRLSREHMKEIFREHDVDGDGFLDYGELTMAFHFLDPVLSCYKARNGIAHADAN 272
            +KG+ M+LSR  +KEIF EHD+DGDG+L   ELT AF F   ++  YKA  G+A+ADA+
Sbjct: 77  EEKGASMKLSRSQIKEIFMEHDIDGDGYLSVSELTKAFSFFGSIIPLYKAHYGLAYADAD 136

Query: 273 GDGLISEDELDKLIDYAE 290
           GDGLISE+EL+KL+DYAE
Sbjct: 137 GDGLISEEELEKLVDYAE 154

[Close]

BLAST of Tan0022016 vs. NCBI nr
Match: XP_022951880.1 (calmodulin-like [Cucurbita moschata])

HSP 1 Score: 104.4 bits (259), Expect = 1.8e-18
Identity = 68/199 (34.17%), Postives = 94/199 (47.24%), Query Frame = 0


Query: 93  FLVAEQVDEFFKKYDKNGDGMITIDELTGIFPQTKNHGSKALPPQNKVNSKAQQQPKEKG 152
           ++     +   KKYDKNGD ++T DELT +                 +  +A QQ     
Sbjct: 14  YMSEADANNLIKKYDKNGDSILTKDELTAM-----------------ILEQASQQ----- 73

Query: 153 KAKANESHANAHKDKEPKKKPDDAKANYESPLHKVSSKANAKPAKKIQDKAQKAPAIELH 212
                                       + P   +  K   KPAK  +D           
Sbjct: 74  ----------------------------QGPPKDIVCKPKKKPAKVPED----------- 133

Query: 213 KKGSIMRLSREHMKEIFREHDVDGDGFLDYGELTMAFHFLDPVLSCYKARNGIAHADANG 272
            KG  MRLSR  +++IF EHD+DGDG+L+  ELT AF F+  +L  YKA  G+ +ADA+G
Sbjct: 134 -KGLHMRLSRSEIRDIFLEHDIDGDGYLNVSELTKAFSFIGSMLPLYKAHYGLNYADADG 150

Query: 273 DGLISEDELDKLIDYAEEL 292
           DG ISE+EL+KL+DYAE +
Sbjct: 194 DGRISEEELEKLVDYAERV 150

[Close]

BLAST of Tan0022016 vs. NCBI nr
Match: XP_022951134.1 (uncharacterized protein LOC111454074 isoform X1 [Cucurbita moschata])

HSP 1 Score: 104.4 bits (259), Expect = 1.8e-18
Identity = 78/200 (39.00%), Postives = 104/200 (52.00%), Query Frame = 0


Query: 91  WQFLVAEQVDEFFKKYDKNGDGMITIDELTGIFPQTKNHGSKALPPQNKVNSKAQQQPKE 150
           +Q    ++ +   KKYD NGDGM+T +EL     Q   H                   K 
Sbjct: 6   FQTCAEDEANRLMKKYDTNGDGMLTKEEL-----QVFLH-------------------KA 65

Query: 151 KGKAKANESHANAHKDKEPKKKPDDAKANYESPLHKVSSKANAKPAKKIQDKAQKAPAIE 210
           +G  + N +       K PK   + A        HK SS ++ K  K++QDK +K+    
Sbjct: 66  RGSTQLNNN--QGFVSKIPKASNNVA--------HKHSSNSHMKHKKQVQDKGKKSSTKF 125

Query: 211 LHKKGSIMRLSREHMKEIFREHDVDGDGFLDYGELTMAFHFLDPVLSCYKARNGIAHADA 270
           L  + S ++LSRE MKEIF+ HD + DGFLD  E+T AF FL  +L   KA   +A+AD 
Sbjct: 126 LDGEMSKLQLSREQMKEIFKNHDSNKDGFLDIKEVTKAFSFLGSILPFNKAHYAMAYADT 171

Query: 271 NGDGLISEDELDKLIDYAEE 291
           N DGLISE ELDKLIDYA +
Sbjct: 186 NHDGLISEFELDKLIDYASK 171

[Close]

BLAST of Tan0022016 vs. ExPASy TrEMBL
Match: A0A6J1KUA8 (calcium-binding protein H-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496471 PE=4 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 7.7e-20
Identity = 78/200 (39.00%), Postives = 101/200 (50.50%), Query Frame = 0


Query: 91  WQFLVAEQVDEFFKKYDKNGDGMITIDELTGIFPQTKNHGSKALPPQNKVNSKAQQQPKE 150
           +Q    ++ +   KKYD NGDGM+T +EL       K  GSK L        K       
Sbjct: 6   FQTWAEDEANRLMKKYDTNGDGMLTKEELQVFL--HKARGSKQLNNNQGFVFKI------ 65

Query: 151 KGKAKANESHANAHKDKEPKKKPDDAKANYESPLHKVSSKANAKPAKKIQDKAQKAPAIE 210
               KAN + A+ H                       SS ++ K  K++QDK +K+    
Sbjct: 66  ---PKANNNVAHEH-----------------------SSNSHMKHKKQVQDKGKKSSTKF 125

Query: 211 LHKKGSIMRLSREHMKEIFREHDVDGDGFLDYGELTMAFHFLDPVLSCYKARNGIAHADA 270
              + S +RLSRE MKEIF+ HD + DGFLD  E+T AF FL  +L   KA   +A+AD 
Sbjct: 126 FDGEMSKLRLSREQMKEIFKNHDSNKDGFLDIKEVTKAFGFLGSILPFNKAHYAMAYADT 171

Query: 271 NGDGLISEDELDKLIDYAEE 291
           N DGLISE ELDKLIDYA +
Sbjct: 186 NHDGLISEFELDKLIDYASK 171

[Close]

BLAST of Tan0022016 vs. ExPASy TrEMBL
Match: A0A6J1KUW2 (calmodulin-like OS=Cucurbita maxima OX=3661 GN=LOC111496626 PE=4 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.3e-19
Identity = 66/139 (47.48%), Postives = 87/139 (62.59%), Query Frame = 0


Query: 153 KAKANESHANAHK--DKEPKKKPDDAKANYESPLHKVSSKANAKPAKKIQDKAQKAPAIE 212
           +A+A E   N  K  D    K+   A  ++    +  S K  AK  K +  KAQK PA  
Sbjct: 17  EAEAQEFIQNYDKNADSSLSKEELSAAIHHNQAKNPGSHKPEAK-KKDVAPKAQKVPAKA 76

Query: 213 LHKKGSIMRLSREHMKEIFREHDVDGDGFLDYGELTMAFHFLDPVLSCYKARNGIAHADA 272
             +KG+ M+LSR  +KEIF EHD+DGDG+L   ELT AF F   ++  YKA  G+A+ADA
Sbjct: 77  AEEKGASMKLSRSQIKEIFMEHDIDGDGYLSVSELTKAFSFFGSIIPLYKAHYGLAYADA 136

Query: 273 NGDGLISEDELDKLIDYAE 290
           +GDGLISE+EL+KL+DYAE
Sbjct: 137 DGDGLISEEELEKLVDYAE 154

[Close]

BLAST of Tan0022016 vs. ExPASy TrEMBL
Match: A0A6J1GK16 (calmodulin-like OS=Cucurbita moschata OX=3662 GN=LOC111454616 PE=4 SV=1)

HSP 1 Score: 107.1 bits (266), Expect = 1.3e-19
Identity = 63/138 (45.65%), Postives = 85/138 (61.59%), Query Frame = 0


Query: 153 KAKANESHANAHKDKEPKKKPDDAKANYESPLHKVSSKANAKPAKK-IQDKAQKAPAIEL 212
           +A+A E   N  K+ +     ++  A       K     N +  KK +  KAQK PA   
Sbjct: 17  EAEAQELMQNYDKNADSSLSKEELSAAIHHNQAKNPGSHNPEAKKKDVAPKAQKVPAKAA 76

Query: 213 HKKGSIMRLSREHMKEIFREHDVDGDGFLDYGELTMAFHFLDPVLSCYKARNGIAHADAN 272
            +KG+ M+LSR  +KEIF EHD+DGDG+L   ELT AF F   ++  YKA  G+A+ADA+
Sbjct: 77  EEKGASMKLSRSQIKEIFMEHDIDGDGYLSVSELTKAFSFFGSIIPLYKAHYGLAYADAD 136

Query: 273 GDGLISEDELDKLIDYAE 290
           GDGLISE+EL+KL+DYAE
Sbjct: 137 GDGLISEEELEKLVDYAE 154

[Close]

BLAST of Tan0022016 vs. ExPASy TrEMBL
Match: A0A6J1GHU7 (uncharacterized protein LOC111454074 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454074 PE=4 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 8.5e-19
Identity = 78/200 (39.00%), Postives = 104/200 (52.00%), Query Frame = 0


Query: 91  WQFLVAEQVDEFFKKYDKNGDGMITIDELTGIFPQTKNHGSKALPPQNKVNSKAQQQPKE 150
           +Q    ++ +   KKYD NGDGM+T +EL     Q   H                   K 
Sbjct: 6   FQTCAEDEANRLMKKYDTNGDGMLTKEEL-----QVFLH-------------------KA 65

Query: 151 KGKAKANESHANAHKDKEPKKKPDDAKANYESPLHKVSSKANAKPAKKIQDKAQKAPAIE 210
           +G  + N +       K PK   + A        HK SS ++ K  K++QDK +K+    
Sbjct: 66  RGSTQLNNN--QGFVSKIPKASNNVA--------HKHSSNSHMKHKKQVQDKGKKSSTKF 125

Query: 211 LHKKGSIMRLSREHMKEIFREHDVDGDGFLDYGELTMAFHFLDPVLSCYKARNGIAHADA 270
           L  + S ++LSRE MKEIF+ HD + DGFLD  E+T AF FL  +L   KA   +A+AD 
Sbjct: 126 LDGEMSKLQLSREQMKEIFKNHDSNKDGFLDIKEVTKAFSFLGSILPFNKAHYAMAYADT 171

Query: 271 NGDGLISEDELDKLIDYAEE 291
           N DGLISE ELDKLIDYA +
Sbjct: 186 NHDGLISEFELDKLIDYASK 171

[Close]

BLAST of Tan0022016 vs. ExPASy TrEMBL
Match: A0A6J1GK54 (calmodulin-like OS=Cucurbita moschata OX=3662 GN=LOC111454617 PE=4 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 8.5e-19
Identity = 68/199 (34.17%), Postives = 94/199 (47.24%), Query Frame = 0


Query: 93  FLVAEQVDEFFKKYDKNGDGMITIDELTGIFPQTKNHGSKALPPQNKVNSKAQQQPKEKG 152
           ++     +   KKYDKNGD ++T DELT +                 +  +A QQ     
Sbjct: 14  YMSEADANNLIKKYDKNGDSILTKDELTAM-----------------ILEQASQQ----- 73

Query: 153 KAKANESHANAHKDKEPKKKPDDAKANYESPLHKVSSKANAKPAKKIQDKAQKAPAIELH 212
                                       + P   +  K   KPAK  +D           
Sbjct: 74  ----------------------------QGPPKDIVCKPKKKPAKVPED----------- 133

Query: 213 KKGSIMRLSREHMKEIFREHDVDGDGFLDYGELTMAFHFLDPVLSCYKARNGIAHADANG 272
            KG  MRLSR  +++IF EHD+DGDG+L+  ELT AF F+  +L  YKA  G+ +ADA+G
Sbjct: 134 -KGLHMRLSRSEIRDIFLEHDIDGDGYLNVSELTKAFSFIGSMLPLYKAHYGLNYADADG 150

Query: 273 DGLISEDELDKLIDYAEEL 292
           DG ISE+EL+KL+DYAE +
Sbjct: 194 DGRISEEELEKLVDYAERV 150

[Close]

BLAST of Tan0022016 vs. TAIR 10
Match: AT1G18530.1 (EF hand calcium-binding protein family )

HSP 1 Score: 43.5 bits (101), Expect = 3.4e-04
Identity = 23/66 (34.85%), Postives = 35/66 (53.03%), Query Frame = 0


Query: 220 LSREHMKEIFREHDVDGDGFLDYGELTMAFHFLDPVLSCYKARNGIAHADANGDGLISED 279
           ++ E + EIF+  D DG+GF+   EL  A   +   L+  +    I  AD NGDG+IS  
Sbjct: 78  INSEQLLEIFKSFDRDGNGFISAAELAGAMAKMGQPLTYKELTEMIKEADTNGDGVISFG 137

Query: 280 ELDKLI 286
           E   ++
Sbjct: 138 EFASIM 143

[Close]

BLAST of Tan0022016 vs. TAIR 10
Match: AT1G50700.1 (calcium-dependent protein kinase 33 )

HSP 1 Score: 42.7 bits (99), Expect = 5.9e-04
Identity = 30/75 (40.00%), Postives = 41/75 (54.67%), Query Frame = 0


Query: 211 LHKKGSIMRLSREHMKEIFREHDVDGDGFLDYGEL---TMAFHFLDPVLSCYKARNGIAH 270
           L K GS  RL+   +K++    DVDG+G +DY E    TM  H L+   + YKA     H
Sbjct: 403 LAKLGS--RLTEAEVKQLMDAADVDGNGSIDYIEFITATMHRHRLESNENVYKA---FQH 462

Query: 271 ADANGDGLISEDELD 283
            D +G G I+ DEL+
Sbjct: 463 FDKDGSGYITTDELE 472

The following BLAST results are available for this feature:

BLAST of Tan0022016 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8RZB5	9.7e-04	37.14	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Tan0022016 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023002688.1	1.6e-19	39.00	calcium-binding protein H-like isoform X1 [Cucurbita maxima]	[more]
XP_023002888.1	2.7e-19	47.48	calmodulin-like [Cucurbita maxima]	[more]
XP_022951879.1	2.7e-19	45.65	calmodulin-like [Cucurbita moschata] >XP_023537873.1 calmodulin-like [Cucurbita ...	[more]
XP_022951880.1	1.8e-18	34.17	calmodulin-like [Cucurbita moschata]	[more]
XP_022951134.1	1.8e-18	39.00	uncharacterized protein LOC111454074 isoform X1 [Cucurbita moschata]	[more]

BLAST of Tan0022016 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KUA8	7.7e-20	39.00	calcium-binding protein H-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1KUW2	1.3e-19	47.48	calmodulin-like OS=Cucurbita maxima OX=3661 GN=LOC111496626 PE=4 SV=1	[more]
A0A6J1GK16	1.3e-19	45.65	calmodulin-like OS=Cucurbita moschata OX=3662 GN=LOC111454616 PE=4 SV=1	[more]
A0A6J1GHU7	8.5e-19	39.00	uncharacterized protein LOC111454074 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1GK54	8.5e-19	34.17	calmodulin-like OS=Cucurbita moschata OX=3662 GN=LOC111454617 PE=4 SV=1	[more]

BLAST of Tan0022016 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18530.1	3.4e-04	34.85	EF hand calcium-binding protein family	[more]
AT1G50700.1	5.9e-04	40.00	calcium-dependent protein kinase 33	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 260..288 e-value: 12.0 score: 9.0 coord: 98..126 e-value: 0.0031 score: 26.7 coord: 224..252 e-value: 0.023 score: 23.8
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 99..122 e-value: 1.1E-6 score: 27.8
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 223..283 e-value: 2.0E-7 score: 31.3
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 94..129 score: 13.676544
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 220..255 score: 14.039183
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 226..285 e-value: 4.93843E-8 score: 47.1573
None	No IPR available	GENE3D	1.10.238.10		coord: 143..295 e-value: 7.8E-14 score: 54.0 coord: 7..142 e-value: 1.0E-5 score: 27.5
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 126..145
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 146..181
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 126..205
None	No IPR available	PANTHER	PTHR10891:SF911	SUBFAMILY NOT NAMED	coord: 211..289
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 211..289
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 233..245
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 269..281
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 107..119
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 94..285

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0022016.1	Tan0022016.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding