Tan0022013 (gene) Snake gourd v1

Overview
NameTan0022013
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionAnhydro-N-acetylmuramic acid kinase
LocationLG01: 2673898 .. 2676849 (+)
RNA-Seq ExpressionTan0022013
SyntenyTan0022013
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAAGCAAAGGTATTGGAGCGGACATGGAAGAACAGCCACAGCTACAGCCTCTGCTACAAAACTCAAAATTAATAATAATAATGGTTTTGATGAAGCTAATTCTTCTTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTACTTCTGCTGGCTGTATGTGTGCTGTTTTTCAGCTCTTTGATTTTCATCCTTTGAATCAACATCATTCTCCTGTTTCTCTCAACCACCCCATTTCTCCTCCACTACATCACCATCTTTCCACAGGTTCCTTCTTTAATCTTTAATTTCTCTTTCATTTTTTTTTCCACCAAAATCAGATTTTTAATTGTGGGTCTGCTAATTTCTTTCTCCAATTTCTCTCTTTAATTCACACCAAAATCGGATTTTGAGAAACTGGGTTAGCTAATCTCTGCAGTCTATATCTATATAATTCTGTCCAAAATCAGATTTTTGAGTAATGGGGTGTTGGAAAATTAATTCAGGTACTGAAGCACCAAGAAACAGTCTGGAATCAGACAAAGAAGAATCTCCATTATCATCTACTCCAAAACAAAAACAAGATGGTCTACATTTCCCAGTAAGTAGTAGTAATTTGATTATATATATTAAAATACCATAATATCCTGTTCAAAAAATTTTAAACGTTAAATTGAATTGGTATTGCAGAAGGGCATTGTTCAAATCAAAACCAAAAGTGGACTCAAAGGATCCGCAGTGAATTCAAAGGAGAATTTGTCCGGAAACTCTCCGAGCACCAAGACGCCAACTTTAGTGGCGAGATTAATGGGTTTGGATCTTCTTCCTGAATCCAACTCTAGTCCTTCTTCAACCAGTACACCAAGAACAGCTTCAGCTTCAGCTTCAGCTTCTCCAATTTACCCAATTCTTCAAAAGCCTAAAACAGCCAATCATTTAATGGGGTCTCGTTCATTGCCAGAAACCCCAAGAACATCTTGTGAAAGAAAATCAAATTATCACCACCGCCTCTCACTTCAAATCCCCAATCATTACGATAAAGAAAACGCAACTCATCATCATACTCCAAGTCCAACCCATTACGCTAAAGAAATCGTGAAGCAAATCAAAGAAACTGTGAGTAGAAAAGCGGGTCTCAACGATATCACGAATTACACAAGAAGAGATCAAGAAGTGCTTATTATTAACCAAACAAAACCAAAAAAGCCGCCATCTTCTTCTTCTTCTTCTTCAGTATCTCCGAGACTGAGAGTACTATTGGACCCCAAGAATTTGAACAAAGCAAGTCCGAAAGTAGTTGAGGCCATTAAAGAAGGTGGTTTAGGGATGAAGGCGAAGCCGAAGCAGAAGGTGAAGACGAAGGTGGCTTTGAAGAAGGGTAGTAAGCAAGAGGAGCCGTTTGTGGTTCCCTCAAGAATCACAAAGGCCGCCATTGATAGTCCTCTTAAGAAGTCTAAGAAAACTCCATTGTCCAATGACCTTCTCACCTTTTCTTCTCTTCCCACCGTTGTGATGAAGAAAGACTCTCCATTTTCCTCCCCCATTAAACCAACTCCAATACAGGTAATTTTTTTTTTTCGTCTTTTTTCAAAATTTATTTATTTTACTTTTTGCATCATTTAATTTAGATCCTATTCAATAACTATTTAAAAATTAAACTTTTTGTTAGACTAGGTTAATATTATTAAATTTACCCCAACCTATCACTTTAAACTTTTAGGTTGATTTATGATTTAAGATAATATCAAAGCAAAAGATCCTAAATTCAAAAATTTGTAAAGAAAGTTTTCTCTCCCAATTAATATGTTTGTTTAAAAACTTAAGTTTTTAGGTTAATTCGCGATGAATTTGATTAAGAATGAATTTTTTTTTTTGTATAAGAGAGATGAAATTTTATCATACATTTTTCGATGGTTAAATTTTGTTCTAGTTTTTAAATTTTTTTTTTTTACAAAATCGTTTGGCTTTCGAAATCTTAAAAATATCTATTTTGGTGTCTCAACTCGGACAAATAATTTTGGTTCAAACAAAGTAATTAAATTTTGTTGGTATGTCTGATTTCATCTTCTTTTTACTTTTTTTTTTTTTTACAGTCACCGTGCAATCAGAATCAAGCTGCAAGTCAGACAGGCGATAAAGAATCAAAACGATACTTGCAGTCATCTAACCACCAACCCCACATTCCAATTATTCATCACCACCACCACGTCATCCAATTACCACCCAATAATAATATCATCACAACCACCTCCACCTCCGCCGCCGCCGCCGCCGACCACCGTAACAGCCACGACATCACGGCAGAGCTGGAGTACGTTAGACAAATACTCCTCCGCCGTAGTACTACTACTTCCTCTTCAGTGTACTCCACCGTTAACGCCCATTATAATGTTTCATTTTCCCATAGAAAATTACTCTCCCACTTGGTCGAAGAGTTGTTGAAGCCCTACTTGGAAGTTAGGCCGTACCGGCAGCCGGCCAAGGAGCGGTGGCCGGAGGTGGTGGACAAATTGTGCGAGAAAGTTAGAAAGTTCCCACGTGCCAAGTGTGAGGTGTTGGAGGACATAGATGGGATAATTGAAAAAGATTTGGACATTTTGGGGATTGGATTTGAGGAGGAAGGTGAAGGGATGGTGAACGAGATTGAGGATTGGATTGTGGAGGAGCTTTTGAATGAAACGGTGCGTTTTATTGAGGAGGCTAAAGACGGTGTCGTTTAAATGGTGCAGACAGAGATGACAGGAGAATCACGTGGGAGATGCCACTTGGATGGTTGGGTCCCACATACCTGGGGTAAAAATGCAGCGTTGGACTTACACGTGGATTCTTGACCCACCAGACAAACACTCAGATTTTTCCGTTCTGAAAAAAGACAGCATTTTTTTGGCGGAGGAATCCCATTCCTCCTCCTCTGTCCTCCTTTGTACAAATTTTATTTCTCTCTCTAAATTCCCCAAAATTAC

mRNA sequence

ATGGGGAAGCAAAGGTATTGGAGCGGACATGGAAGAACAGCCACAGCTACAGCCTCTGCTACAAAACTCAAAATTAATAATAATAATGGTTTTGATGAAGCTAATTCTTCTTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTACTTCTGCTGGCTGTATGTGTGCTGTTTTTCAGCTCTTTGATTTTCATCCTTTGAATCAACATCATTCTCCTGTTTCTCTCAACCACCCCATTTCTCCTCCACTACATCACCATCTTTCCACAGGTACTGAAGCACCAAGAAACAGTCTGGAATCAGACAAAGAAGAATCTCCATTATCATCTACTCCAAAACAAAAACAAGATGGTCTACATTTCCCAAAGGGCATTGTTCAAATCAAAACCAAAAGTGGACTCAAAGGATCCGCAGTGAATTCAAAGGAGAATTTGTCCGGAAACTCTCCGAGCACCAAGACGCCAACTTTAGTGGCGAGATTAATGGGTTTGGATCTTCTTCCTGAATCCAACTCTAGTCCTTCTTCAACCAGTACACCAAGAACAGCTTCAGCTTCAGCTTCAGCTTCTCCAATTTACCCAATTCTTCAAAAGCCTAAAACAGCCAATCATTTAATGGGGTCTCGTTCATTGCCAGAAACCCCAAGAACATCTTGTGAAAGAAAATCAAATTATCACCACCGCCTCTCACTTCAAATCCCCAATCATTACGATAAAGAAAACGCAACTCATCATCATACTCCAAGTCCAACCCATTACGCTAAAGAAATCGTGAAGCAAATCAAAGAAACTGTGAGTAGAAAAGCGGGTCTCAACGATATCACGAATTACACAAGAAGAGATCAAGAAGTGCTTATTATTAACCAAACAAAACCAAAAAAGCCGCCATCTTCTTCTTCTTCTTCTTCAGTATCTCCGAGACTGAGAGTACTATTGGACCCCAAGAATTTGAACAAAGCAAGTCCGAAAGTAGTTGAGGCCATTAAAGAAGGTGGTTTAGGGATGAAGGCGAAGCCGAAGCAGAAGGTGAAGACGAAGGTGGCTTTGAAGAAGGGTAGTAAGCAAGAGGAGCCGTTTGTGGTTCCCTCAAGAATCACAAAGGCCGCCATTGATAGTCCTCTTAAGAAGTCTAAGAAAACTCCATTGTCCAATGACCTTCTCACCTTTTCTTCTCTTCCCACCGTTGTGATGAAGAAAGACTCTCCATTTTCCTCCCCCATTAAACCAACTCCAATACAGTCACCGTGCAATCAGAATCAAGCTGCAAGTCAGACAGGCGATAAAGAATCAAAACGATACTTGCAGTCATCTAACCACCAACCCCACATTCCAATTATTCATCACCACCACCACGTCATCCAATTACCACCCAATAATAATATCATCACAACCACCTCCACCTCCGCCGCCGCCGCCGCCGACCACCGTAACAGCCACGACATCACGGCAGAGCTGGAGTACGTTAGACAAATACTCCTCCGCCGTAGTACTACTACTTCCTCTTCAGTGTACTCCACCGTTAACGCCCATTATAATGTTTCATTTTCCCATAGAAAATTACTCTCCCACTTGGTCGAAGAGTTGTTGAAGCCCTACTTGGAAGTTAGGCCGTACCGGCAGCCGGCCAAGGAGCGGTGGCCGGAGGTGGTGGACAAATTGTGCGAGAAAGTTAGAAAGTTCCCACGTGCCAAGTGTGAGGTGTTGGAGGACATAGATGGGATAATTGAAAAAGATTTGGACATTTTGGGGATTGGATTTGAGGAGGAAGGTGAAGGGATGGTGAACGAGATTGAGGATTGGATTGTGGAGGAGCTTTTGAATGAAACGGTGCGTTTTATTGAGGAGGCTAAAGACGGTGTCGTTTAAATGGTGCAGACAGAGATGACAGGAGAATCACGTGGGAGATGCCACTTGGATGGTTGGGTCCCACATACCTGGGGTAAAAATGCAGCGTTGGACTTACACGTGGATTCTTGACCCACCAGACAAACACTCAGATTTTTCCGTTCTGAAAAAAGACAGCATTTTTTTGGCGGAGGAATCCCATTCCTCCTCCTCTGTCCTCCTTTGTACAAATTTTATTTCTCTCTCTAAATTCCCCAAAATTAC

Coding sequence (CDS)

ATGGGGAAGCAAAGGTATTGGAGCGGACATGGAAGAACAGCCACAGCTACAGCCTCTGCTACAAAACTCAAAATTAATAATAATAATGGTTTTGATGAAGCTAATTCTTCTTTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTACTTCTGCTGGCTGTATGTGTGCTGTTTTTCAGCTCTTTGATTTTCATCCTTTGAATCAACATCATTCTCCTGTTTCTCTCAACCACCCCATTTCTCCTCCACTACATCACCATCTTTCCACAGGTACTGAAGCACCAAGAAACAGTCTGGAATCAGACAAAGAAGAATCTCCATTATCATCTACTCCAAAACAAAAACAAGATGGTCTACATTTCCCAAAGGGCATTGTTCAAATCAAAACCAAAAGTGGACTCAAAGGATCCGCAGTGAATTCAAAGGAGAATTTGTCCGGAAACTCTCCGAGCACCAAGACGCCAACTTTAGTGGCGAGATTAATGGGTTTGGATCTTCTTCCTGAATCCAACTCTAGTCCTTCTTCAACCAGTACACCAAGAACAGCTTCAGCTTCAGCTTCAGCTTCTCCAATTTACCCAATTCTTCAAAAGCCTAAAACAGCCAATCATTTAATGGGGTCTCGTTCATTGCCAGAAACCCCAAGAACATCTTGTGAAAGAAAATCAAATTATCACCACCGCCTCTCACTTCAAATCCCCAATCATTACGATAAAGAAAACGCAACTCATCATCATACTCCAAGTCCAACCCATTACGCTAAAGAAATCGTGAAGCAAATCAAAGAAACTGTGAGTAGAAAAGCGGGTCTCAACGATATCACGAATTACACAAGAAGAGATCAAGAAGTGCTTATTATTAACCAAACAAAACCAAAAAAGCCGCCATCTTCTTCTTCTTCTTCTTCAGTATCTCCGAGACTGAGAGTACTATTGGACCCCAAGAATTTGAACAAAGCAAGTCCGAAAGTAGTTGAGGCCATTAAAGAAGGTGGTTTAGGGATGAAGGCGAAGCCGAAGCAGAAGGTGAAGACGAAGGTGGCTTTGAAGAAGGGTAGTAAGCAAGAGGAGCCGTTTGTGGTTCCCTCAAGAATCACAAAGGCCGCCATTGATAGTCCTCTTAAGAAGTCTAAGAAAACTCCATTGTCCAATGACCTTCTCACCTTTTCTTCTCTTCCCACCGTTGTGATGAAGAAAGACTCTCCATTTTCCTCCCCCATTAAACCAACTCCAATACAGTCACCGTGCAATCAGAATCAAGCTGCAAGTCAGACAGGCGATAAAGAATCAAAACGATACTTGCAGTCATCTAACCACCAACCCCACATTCCAATTATTCATCACCACCACCACGTCATCCAATTACCACCCAATAATAATATCATCACAACCACCTCCACCTCCGCCGCCGCCGCCGCCGACCACCGTAACAGCCACGACATCACGGCAGAGCTGGAGTACGTTAGACAAATACTCCTCCGCCGTAGTACTACTACTTCCTCTTCAGTGTACTCCACCGTTAACGCCCATTATAATGTTTCATTTTCCCATAGAAAATTACTCTCCCACTTGGTCGAAGAGTTGTTGAAGCCCTACTTGGAAGTTAGGCCGTACCGGCAGCCGGCCAAGGAGCGGTGGCCGGAGGTGGTGGACAAATTGTGCGAGAAAGTTAGAAAGTTCCCACGTGCCAAGTGTGAGGTGTTGGAGGACATAGATGGGATAATTGAAAAAGATTTGGACATTTTGGGGATTGGATTTGAGGAGGAAGGTGAAGGGATGGTGAACGAGATTGAGGATTGGATTGTGGAGGAGCTTTTGAATGAAACGGTGCGTTTTATTGAGGAGGCTAAAGACGGTGTCGTTTAA

Protein sequence

MGKQRYWSGHGRTATATASATKLKINNNNGFDEANSSFSSSSSSSSSSSSSTSAGCMCAVFQLFDFHPLNQHHSPVSLNHPISPPLHHHLSTGTEAPRNSLESDKEESPLSSTPKQKQDGLHFPKGIVQIKTKSGLKGSAVNSKENLSGNSPSTKTPTLVARLMGLDLLPESNSSPSSTSTPRTASASASASPIYPILQKPKTANHLMGSRSLPETPRTSCERKSNYHHRLSLQIPNHYDKENATHHHTPSPTHYAKEIVKQIKETVSRKAGLNDITNYTRRDQEVLIINQTKPKKPPSSSSSSSVSPRLRVLLDPKNLNKASPKVVEAIKEGGLGMKAKPKQKVKTKVALKKGSKQEEPFVVPSRITKAAIDSPLKKSKKTPLSNDLLTFSSLPTVVMKKDSPFSSPIKPTPIQSPCNQNQAASQTGDKESKRYLQSSNHQPHIPIIHHHHHVIQLPPNNNIITTTSTSAAAAADHRNSHDITAELEYVRQILLRRSTTTSSSVYSTVNAHYNVSFSHRKLLSHLVEELLKPYLEVRPYRQPAKERWPEVVDKLCEKVRKFPRAKCEVLEDIDGIIEKDLDILGIGFEEEGEGMVNEIEDWIVEELLNETVRFIEEAKDGVV
Homology
BLAST of Tan0022013 vs. NCBI nr
Match: XP_022984435.1 (uncharacterized protein LOC111482735 isoform X3 [Cucurbita maxima])

HSP 1 Score: 723.4 bits (1866), Expect = 1.7e-204
Identity = 442/650 (68.00%), Postives = 492/650 (75.69%), Query Frame = 0

Query: 1   MGKQRYWSGHGRTATATASATKLKINNNNGFDEANSSFSSSSSSSSSSSSSTSAGCMCAV 60
           MGKQRYWSGHGRTATA   +TK   N  NGFDEA              SSSTSAGCMCAV
Sbjct: 1   MGKQRYWSGHGRTATA---STKTLKNEPNGFDEA--------------SSSTSAGCMCAV 60

Query: 61  FQLFDFHPL--NQHHSP----VSLNHPISPPL--HHHLSTGTEAPRNSLESDKEESPLSS 120
           FQLFDFHPL  +QHH P    VSLN  +SPP     HLS GTEAPRNS+ES++EES L  
Sbjct: 61  FQLFDFHPLSHHQHHHPPRSTVSLNPIVSPPPPDDDHLSKGTEAPRNSVESEEEESSLPC 120

Query: 121 TPKQKQDGLHFPKGIVQIKTKSGL-KGSAVNSKENLS--GNSPSTKTPTLVARLMGLDLL 180
           TPKQK+DGLHFPKGIVQIKTKSG+ K S +NS +NLS   +SPSTKTPTLVARLMGLDLL
Sbjct: 121 TPKQKEDGLHFPKGIVQIKTKSGITKESEMNSNKNLSAGNDSPSTKTPTLVARLMGLDLL 180

Query: 181 PESNSSPSSTSTPRTASASASASPIYPILQKPKTA-NHLMGSRSLPETPRTSCERK---S 240
           P+S S  ++T TP            Y ++ KPKT  NHL G+RSLPETPRTSCERK    
Sbjct: 181 PQSTSPSTTTRTP------------YSLVGKPKTGKNHLTGTRSLPETPRTSCERKPNVD 240

Query: 241 NYHHRLSLQIPNHYDKENATHHHTPSPTHYAKEIVKQIKETVSRKAGLNDITN---YTRR 300
           NYHHRLSLQIPN +DKENAT   +P+P+HYAKEIVKQIKETVSRK GL+DITN    TRR
Sbjct: 241 NYHHRLSLQIPN-FDKENATPSPSPNPSHYAKEIVKQIKETVSRKGGLSDITNNYYSTRR 300

Query: 301 DQEVLIINQTKPKKPPSSSSSSSVSPRLRVLLDPKNLNKASPKVVEAIKEGGLGMKAKPK 360
           DQ+  +I QTKPKK  S SSSSSVSPRL     PKN NK +PK VEA+KEG LG +    
Sbjct: 301 DQD--MITQTKPKKVLSLSSSSSVSPRL-----PKNPNKPTPK-VEAMKEGVLGQRKPRT 360

Query: 361 QK-VKTKVALKKGSKQEEPFVVPSRITKAAIDSPLKKSKKTPLSNDLLTFSSLPTVVMKK 420
           QK VKTK  LKK +KQEEPFVVPSRITKAAID PLK +KKTPLSN LL+F S+PT++MKK
Sbjct: 361 QKPVKTKAGLKKSNKQEEPFVVPSRITKAAIDGPLKNTKKTPLSNQLLSFGSVPTILMKK 420

Query: 421 DSPFSSPIKPTPIQSPCNQNQAASQTGDKESKRYLQSSNHQPHIPIIHHHHHVIQLP-PN 480
           D PF SPIKP P+QSPCN+NQ A+QT DKESKRYLQSSNHQPHIPIIHHH HVIQLP  N
Sbjct: 421 DPPFPSPIKPFPMQSPCNRNQPANQTVDKESKRYLQSSNHQPHIPIIHHHQHVIQLPLSN 480

Query: 481 NNIITTTSTSAAAAADHRNSHDITAELEYVRQILLRRSTTTSSSVYSTV------NAHYN 540
           +N         AAAAD   +HDI AELEYVRQILLRR  +TS+SVYSTV        H  
Sbjct: 481 SNKDGNNPKQNAAAADCSGNHDIAAELEYVRQILLRR-RSTSTSVYSTVFPENYNTTHNR 540

Query: 541 VSFSHRKLLSHLVEELLKPYLEVRPYRQPAK--ERWPEVVDKLCEKVRKFPRAKCEVLED 600
           VS SHRKLL HLVEELL+PYLEVRPYR  A   E W +VV+KLCEKV++ PRAKCE+LED
Sbjct: 541 VSNSHRKLLCHLVEELLEPYLEVRPYRGAASPGEVWADVVEKLCEKVKRLPRAKCEILED 600

Query: 601 IDGIIEKDLDILGIGFEEEG-EGMVNEIEDWIVEELLNETVRFIEEAKDG 622
           IDGIIEKD+DILGIGFEEEG EG+V EIE+WIVEELLNETVRF+E    G
Sbjct: 601 IDGIIEKDMDILGIGFEEEGEEGIVKEIEEWIVEELLNETVRFVETEMAG 611

BLAST of Tan0022013 vs. NCBI nr
Match: XP_022984434.1 (uncharacterized protein LOC111482735 isoform X2 [Cucurbita maxima])

HSP 1 Score: 717.6 bits (1851), Expect = 9.2e-203
Identity = 442/654 (67.58%), Postives = 492/654 (75.23%), Query Frame = 0

Query: 1   MGKQRYWSGHGRTATATASATKLKINNNNGFDEANSSFSSSSSSSSSSSSSTSAGCMCAV 60
           MGKQRYWSGHGRTATA   +TK   N  NGFDEA              SSSTSAGCMCAV
Sbjct: 1   MGKQRYWSGHGRTATA---STKTLKNEPNGFDEA--------------SSSTSAGCMCAV 60

Query: 61  FQLFDFHPL--NQHHSP----VSLNHPISPPL--HHHLS----TGTEAPRNSLESDKEES 120
           FQLFDFHPL  +QHH P    VSLN  +SPP     HLS     GTEAPRNS+ES++EES
Sbjct: 61  FQLFDFHPLSHHQHHHPPRSTVSLNPIVSPPPPDDDHLSKVKNLGTEAPRNSVESEEEES 120

Query: 121 PLSSTPKQKQDGLHFPKGIVQIKTKSGL-KGSAVNSKENLS--GNSPSTKTPTLVARLMG 180
            L  TPKQK+DGLHFPKGIVQIKTKSG+ K S +NS +NLS   +SPSTKTPTLVARLMG
Sbjct: 121 SLPCTPKQKEDGLHFPKGIVQIKTKSGITKESEMNSNKNLSAGNDSPSTKTPTLVARLMG 180

Query: 181 LDLLPESNSSPSSTSTPRTASASASASPIYPILQKPKTA-NHLMGSRSLPETPRTSCERK 240
           LDLLP+S S  ++T TP            Y ++ KPKT  NHL G+RSLPETPRTSCERK
Sbjct: 181 LDLLPQSTSPSTTTRTP------------YSLVGKPKTGKNHLTGTRSLPETPRTSCERK 240

Query: 241 ---SNYHHRLSLQIPNHYDKENATHHHTPSPTHYAKEIVKQIKETVSRKAGLNDITN--- 300
               NYHHRLSLQIPN +DKENAT   +P+P+HYAKEIVKQIKETVSRK GL+DITN   
Sbjct: 241 PNVDNYHHRLSLQIPN-FDKENATPSPSPNPSHYAKEIVKQIKETVSRKGGLSDITNNYY 300

Query: 301 YTRRDQEVLIINQTKPKKPPSSSSSSSVSPRLRVLLDPKNLNKASPKVVEAIKEGGLGMK 360
            TRRDQ+  +I QTKPKK  S SSSSSVSPRL     PKN NK +PK VEA+KEG LG +
Sbjct: 301 STRRDQD--MITQTKPKKVLSLSSSSSVSPRL-----PKNPNKPTPK-VEAMKEGVLGQR 360

Query: 361 AKPKQK-VKTKVALKKGSKQEEPFVVPSRITKAAIDSPLKKSKKTPLSNDLLTFSSLPTV 420
               QK VKTK  LKK +KQEEPFVVPSRITKAAID PLK +KKTPLSN LL+F S+PT+
Sbjct: 361 KPRTQKPVKTKAGLKKSNKQEEPFVVPSRITKAAIDGPLKNTKKTPLSNQLLSFGSVPTI 420

Query: 421 VMKKDSPFSSPIKPTPIQSPCNQNQAASQTGDKESKRYLQSSNHQPHIPIIHHHHHVIQL 480
           +MKKD PF SPIKP P+QSPCN+NQ A+QT DKESKRYLQSSNHQPHIPIIHHH HVIQL
Sbjct: 421 LMKKDPPFPSPIKPFPMQSPCNRNQPANQTVDKESKRYLQSSNHQPHIPIIHHHQHVIQL 480

Query: 481 P-PNNNIITTTSTSAAAAADHRNSHDITAELEYVRQILLRRSTTTSSSVYSTV------N 540
           P  N+N         AAAAD   +HDI AELEYVRQILLRR  +TS+SVYSTV       
Sbjct: 481 PLSNSNKDGNNPKQNAAAADCSGNHDIAAELEYVRQILLRR-RSTSTSVYSTVFPENYNT 540

Query: 541 AHYNVSFSHRKLLSHLVEELLKPYLEVRPYRQPAK--ERWPEVVDKLCEKVRKFPRAKCE 600
            H  VS SHRKLL HLVEELL+PYLEVRPYR  A   E W +VV+KLCEKV++ PRAKCE
Sbjct: 541 THNRVSNSHRKLLCHLVEELLEPYLEVRPYRGAASPGEVWADVVEKLCEKVKRLPRAKCE 600

Query: 601 VLEDIDGIIEKDLDILGIGFEEEG-EGMVNEIEDWIVEELLNETVRFIEEAKDG 622
           +LEDIDGIIEKD+DILGIGFEEEG EG+V EIE+WIVEELLNETVRF+E    G
Sbjct: 601 ILEDIDGIIEKDMDILGIGFEEEGEEGIVKEIEEWIVEELLNETVRFVETEMAG 615

BLAST of Tan0022013 vs. NCBI nr
Match: KAG6576756.1 (hypothetical protein SDJN03_24330, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 715.7 bits (1846), Expect = 3.5e-202
Identity = 438/653 (67.08%), Postives = 490/653 (75.04%), Query Frame = 0

Query: 1   MGKQRYWSGHGRTATATASATKLKINNNNGFDEANSSFSSSSSSSSSSSSSTSAGCMCAV 60
           MGKQRYWSGHGRTATA   +TK   N  NGFDEA              SSSTSAGCMCAV
Sbjct: 1   MGKQRYWSGHGRTATA---STKTLKNQPNGFDEA--------------SSSTSAGCMCAV 60

Query: 61  FQLFDFHPL--NQHHSP----VSLNHPISPPL--HHHLSTGTEAPRNSLESDKEESPLSS 120
           FQLFDFHPL  +QHH P    VSLN  +SPP     HLS GTEAPRNS+ES++EES L  
Sbjct: 61  FQLFDFHPLSHHQHHHPPRSTVSLNPIVSPPPPDDDHLSKGTEAPRNSVESEEEESSLPC 120

Query: 121 TPKQKQDGLHFPKGIVQIKTKSGL-KGSAVNSKENLS--GNSPSTKTPTLVARLMGLDLL 180
           TPKQK+DGLHFPKGIVQIKTKSG+ K S +NS +NLS   +SPSTKTPTLVARLMGLDLL
Sbjct: 121 TPKQKEDGLHFPKGIVQIKTKSGITKESEMNSNKNLSAGNDSPSTKTPTLVARLMGLDLL 180

Query: 181 PESNSSPSSTSTPRTASASASASPIYPILQKPKTA-NHLMGSRSLPETPRTSCERK---S 240
           P+S S  ++T TP            + ++ KPKT  NHL G+RSLPETPRTSCERK    
Sbjct: 181 PQSTSPSTTTRTP------------HSVVGKPKTGKNHLTGTRSLPETPRTSCERKPNVD 240

Query: 241 NYHHRLSLQIPNHYDKENATHHHTPSPTHYAKEIVKQIKETVSRKAGLNDITN---YTRR 300
           NYHHRLSLQIPN +DKENA+    P+P+HYAKEIVKQIKETVSRK GL+DITN    TRR
Sbjct: 241 NYHHRLSLQIPN-FDKENASPSPGPNPSHYAKEIVKQIKETVSRKGGLSDITNNYYSTRR 300

Query: 301 DQEVLIINQTKPKKPPSSSSSSSVSPRLRVLLDPKNLNKASPKVVEAIKEGGLGMKAKPK 360
           DQ+  II QTKPKK  S SSSSSVSPRL     PKN NK +PK VEA+KEG LG +   +
Sbjct: 301 DQD--IITQTKPKKVISLSSSSSVSPRL-----PKNPNKQTPK-VEAMKEGVLGQRKPKR 360

Query: 361 QK-VKTKVALKKGSKQEEPFVVPSRITKAAIDSPLKKSKKTPLSNDLLTFSSLPTVVMKK 420
           QK VKTK  LKK +KQEEPFVVPSRITKAAID PLK +KKTPLSN LL F S+PT+VMKK
Sbjct: 361 QKPVKTKAGLKKSNKQEEPFVVPSRITKAAIDGPLKNTKKTPLSNQLLNFGSVPTIVMKK 420

Query: 421 DSPFSSPIKPTPIQSPCNQNQAASQTGDKESKRYLQSSNHQPHIPIIHHHHHVIQLPPNN 480
           D PF SPIKP P+QSPCN+NQ A+QT DKESKRYLQSSNHQPHI IIHHH HVIQLP +N
Sbjct: 421 DPPFPSPIKPFPMQSPCNRNQPANQTVDKESKRYLQSSNHQPHISIIHHHQHVIQLPLSN 480

Query: 481 N----IITTTSTSAAAAADHRNSHDITAELEYVRQILLRRSTTTSSSVYSTV-------N 540
           +         + + AAAAD   +HDI AELEY+RQILLRR  TTSSSVYSTV        
Sbjct: 481 SNKDGNNPKQNAAVAAAADCSYNHDIAAELEYIRQILLRR-RTTSSSVYSTVFLPENYNT 540

Query: 541 AHYNVSFSHRKLLSHLVEELLKPYLEVRPYRQPAK--ERWPEVVDKLCEKVRKFPRAKCE 600
            H  VS SHRKLL HLVEELL+PYLE RPYR+ A   E W +VV+KLCEKV++ PRAKCE
Sbjct: 541 THNRVSNSHRKLLCHLVEELLEPYLEARPYRKAASPGEVWTDVVEKLCEKVKRLPRAKCE 600

Query: 601 VLEDIDGIIEKDLDILGIGFEEEGEGMVNEIEDWIVEELLNETVRFIEEAKDG 622
           +LEDIDGIIEKD+DILGIGFEEEGEG+V EIE+ IVEELLNETVR +E    G
Sbjct: 601 ILEDIDGIIEKDMDILGIGFEEEGEGIVKEIEECIVEELLNETVRLVETEMAG 614

BLAST of Tan0022013 vs. NCBI nr
Match: XP_022984433.1 (uncharacterized protein LOC111482735 isoform X1 [Cucurbita maxima])

HSP 1 Score: 713.8 bits (1841), Expect = 1.3e-201
Identity = 442/664 (66.57%), Postives = 492/664 (74.10%), Query Frame = 0

Query: 1   MGKQRYWSGHGRTATATASATKLKINNNNGFDEANSSFSSSSSSSSSSSSSTSAGCMCAV 60
           MGKQRYWSGHGRTATA   +TK   N  NGFDEA              SSSTSAGCMCAV
Sbjct: 1   MGKQRYWSGHGRTATA---STKTLKNEPNGFDEA--------------SSSTSAGCMCAV 60

Query: 61  FQLFDFHPL--NQHHSP----VSLNHPISPPL--HHHLS--------------TGTEAPR 120
           FQLFDFHPL  +QHH P    VSLN  +SPP     HLS               GTEAPR
Sbjct: 61  FQLFDFHPLSHHQHHHPPRSTVSLNPIVSPPPPDDDHLSKDQNLILSNGSVKNLGTEAPR 120

Query: 121 NSLESDKEESPLSSTPKQKQDGLHFPKGIVQIKTKSGL-KGSAVNSKENLS--GNSPSTK 180
           NS+ES++EES L  TPKQK+DGLHFPKGIVQIKTKSG+ K S +NS +NLS   +SPSTK
Sbjct: 121 NSVESEEEESSLPCTPKQKEDGLHFPKGIVQIKTKSGITKESEMNSNKNLSAGNDSPSTK 180

Query: 181 TPTLVARLMGLDLLPESNSSPSSTSTPRTASASASASPIYPILQKPKTA-NHLMGSRSLP 240
           TPTLVARLMGLDLLP+S S  ++T TP            Y ++ KPKT  NHL G+RSLP
Sbjct: 181 TPTLVARLMGLDLLPQSTSPSTTTRTP------------YSLVGKPKTGKNHLTGTRSLP 240

Query: 241 ETPRTSCERK---SNYHHRLSLQIPNHYDKENATHHHTPSPTHYAKEIVKQIKETVSRKA 300
           ETPRTSCERK    NYHHRLSLQIPN +DKENAT   +P+P+HYAKEIVKQIKETVSRK 
Sbjct: 241 ETPRTSCERKPNVDNYHHRLSLQIPN-FDKENATPSPSPNPSHYAKEIVKQIKETVSRKG 300

Query: 301 GLNDITN---YTRRDQEVLIINQTKPKKPPSSSSSSSVSPRLRVLLDPKNLNKASPKVVE 360
           GL+DITN    TRRDQ+  +I QTKPKK  S SSSSSVSPRL     PKN NK +PK VE
Sbjct: 301 GLSDITNNYYSTRRDQD--MITQTKPKKVLSLSSSSSVSPRL-----PKNPNKPTPK-VE 360

Query: 361 AIKEGGLGMKAKPKQK-VKTKVALKKGSKQEEPFVVPSRITKAAIDSPLKKSKKTPLSND 420
           A+KEG LG +    QK VKTK  LKK +KQEEPFVVPSRITKAAID PLK +KKTPLSN 
Sbjct: 361 AMKEGVLGQRKPRTQKPVKTKAGLKKSNKQEEPFVVPSRITKAAIDGPLKNTKKTPLSNQ 420

Query: 421 LLTFSSLPTVVMKKDSPFSSPIKPTPIQSPCNQNQAASQTGDKESKRYLQSSNHQPHIPI 480
           LL+F S+PT++MKKD PF SPIKP P+QSPCN+NQ A+QT DKESKRYLQSSNHQPHIPI
Sbjct: 421 LLSFGSVPTILMKKDPPFPSPIKPFPMQSPCNRNQPANQTVDKESKRYLQSSNHQPHIPI 480

Query: 481 IHHHHHVIQLP-PNNNIITTTSTSAAAAADHRNSHDITAELEYVRQILLRRSTTTSSSVY 540
           IHHH HVIQLP  N+N         AAAAD   +HDI AELEYVRQILLRR  +TS+SVY
Sbjct: 481 IHHHQHVIQLPLSNSNKDGNNPKQNAAAADCSGNHDIAAELEYVRQILLRR-RSTSTSVY 540

Query: 541 STV------NAHYNVSFSHRKLLSHLVEELLKPYLEVRPYRQPAK--ERWPEVVDKLCEK 600
           STV        H  VS SHRKLL HLVEELL+PYLEVRPYR  A   E W +VV+KLCEK
Sbjct: 541 STVFPENYNTTHNRVSNSHRKLLCHLVEELLEPYLEVRPYRGAASPGEVWADVVEKLCEK 600

Query: 601 VRKFPRAKCEVLEDIDGIIEKDLDILGIGFEEEG-EGMVNEIEDWIVEELLNETVRFIEE 622
           V++ PRAKCE+LEDIDGIIEKD+DILGIGFEEEG EG+V EIE+WIVEELLNETVRF+E 
Sbjct: 601 VKRLPRAKCEILEDIDGIIEKDMDILGIGFEEEGEEGIVKEIEEWIVEELLNETVRFVET 625

BLAST of Tan0022013 vs. NCBI nr
Match: XP_023553057.1 (uncharacterized protein LOC111810568 isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 711.4 bits (1835), Expect = 6.6e-201
Identity = 435/648 (67.13%), Postives = 490/648 (75.62%), Query Frame = 0

Query: 1   MGKQRYWSGHGRTATATASATKLKINNNNGFDEANSSFSSSSSSSSSSSSSTSAGCMCAV 60
           MGKQRYWSGHGRTATA   +TK   N  NGFDEA              SSSTSAGCMCAV
Sbjct: 1   MGKQRYWSGHGRTATA---STKTLKNQPNGFDEA--------------SSSTSAGCMCAV 60

Query: 61  FQLFDFHPL--NQHHSP----VSLNHPISPPL--HHHLSTGTEAPRNSLESDKEESPLSS 120
           FQLFDFHPL  +QHH P    VSLN  +SPP     HLS G EAPRNS+ES++EES L  
Sbjct: 61  FQLFDFHPLSHHQHHHPPPSTVSLNPIVSPPPPDDDHLSKGIEAPRNSVESEEEESSLPC 120

Query: 121 TPKQKQDGLHFPKGIVQIKTKSGL-KGSAVNSKENLS--GNSPSTKTPTLVARLMGLDLL 180
           TPKQK+DGLHFPKGIVQIKTKSG+ K S +NS +NLS   +SPSTKTPTLVARLMGLDLL
Sbjct: 121 TPKQKEDGLHFPKGIVQIKTKSGITKESEMNSNKNLSAGNDSPSTKTPTLVARLMGLDLL 180

Query: 181 PESNSSPSSTSTPRTASASASASPIYPILQKPKTA-NHLMGSRSLPETPRTSCERK---S 240
           P+S S  +++ TP            Y ++ KPKT  NHL G+RSLPETPRTSCERK    
Sbjct: 181 PQSTSPSTTSRTP------------YSLVGKPKTGKNHLTGTRSLPETPRTSCERKPNVD 240

Query: 241 NYHHRLSLQIPNHYDKENATHHHTPSPTHYAKEIVKQIKETVSRKAGLNDITN---YTRR 300
           NYHHRLSLQIPN +DKENA+   +P+P+HYAKEIVKQIKETVSRK GL+DITN    TRR
Sbjct: 241 NYHHRLSLQIPN-FDKENAS--PSPNPSHYAKEIVKQIKETVSRKGGLSDITNNYYSTRR 300

Query: 301 DQEVLIINQTKPKKPPSSSSSSSVSPRLRVLLDPKNLNKASPKVVEAIKEGGLGMKAKPK 360
           DQ+  II QTKPKK  S SSSSSVSPRL     PKN NK +PK VEA+KEG LG +   +
Sbjct: 301 DQD--IITQTKPKKVISLSSSSSVSPRL-----PKNPNKQTPK-VEAMKEGVLGQRKPKR 360

Query: 361 QK-VKTKVALKKGSKQEEPFVVPSRITKAAIDSPLKKSKKTPLSNDLLTFSSLPTVVMKK 420
           QK VKTK  LKK +KQEEPFVVPSRITKAAID PLK +KKTPLSN LL F S+PT+VMKK
Sbjct: 361 QKPVKTKAGLKKSNKQEEPFVVPSRITKAAIDGPLKNTKKTPLSNQLLNFGSVPTIVMKK 420

Query: 421 DSPFSSPIKPTPIQSPCNQNQAASQTGDKESKRYLQSSNHQPHIPIIHHHHHVIQLPPNN 480
           D PF SPIKP+P+QSPCN+NQ A+QT DKESKRYLQSSNHQPHIPIIHHH HVIQ P +N
Sbjct: 421 DPPFPSPIKPSPMQSPCNRNQPANQTVDKESKRYLQSSNHQPHIPIIHHHQHVIQSPLSN 480

Query: 481 NIITTTSTSAAAAADHRNSHDITAELEYVRQILLRRSTTTSSSVYSTV------NAHYNV 540
           +     +     AAD   +HDI AELEY+RQILLRR  +TSSSVYSTV        H  V
Sbjct: 481 S-NKDGNNPKQNAADCSYNHDIAAELEYIRQILLRR-RSTSSSVYSTVFPENYNTTHNRV 540

Query: 541 SFSHRKLLSHLVEELLKPYLEVRPYRQPAK--ERWPEVVDKLCEKVRKFPRAKCEVLEDI 600
           S SHRKLL HLVEELL+PYLEVRPYR+ A   E W +VV+KLCEKV++ PRAKCE+LEDI
Sbjct: 541 SNSHRKLLCHLVEELLEPYLEVRPYRKAASPGEVWADVVEKLCEKVKRLPRAKCEILEDI 600

Query: 601 DGIIEKDLDILGIGFEEEGEGMVNEIEDWIVEELLNETVRFIEEAKDG 622
           DGIIEKD++ILGIGFEEEGEG+V EIE+ IVEELLNETVRF+E    G
Sbjct: 601 DGIIEKDMEILGIGFEEEGEGIVKEIEECIVEELLNETVRFVETEMAG 606

BLAST of Tan0022013 vs. ExPASy TrEMBL
Match: A0A6J1J8L6 (uncharacterized protein LOC111482735 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111482735 PE=4 SV=1)

HSP 1 Score: 723.4 bits (1866), Expect = 8.1e-205
Identity = 442/650 (68.00%), Postives = 492/650 (75.69%), Query Frame = 0

Query: 1   MGKQRYWSGHGRTATATASATKLKINNNNGFDEANSSFSSSSSSSSSSSSSTSAGCMCAV 60
           MGKQRYWSGHGRTATA   +TK   N  NGFDEA              SSSTSAGCMCAV
Sbjct: 1   MGKQRYWSGHGRTATA---STKTLKNEPNGFDEA--------------SSSTSAGCMCAV 60

Query: 61  FQLFDFHPL--NQHHSP----VSLNHPISPPL--HHHLSTGTEAPRNSLESDKEESPLSS 120
           FQLFDFHPL  +QHH P    VSLN  +SPP     HLS GTEAPRNS+ES++EES L  
Sbjct: 61  FQLFDFHPLSHHQHHHPPRSTVSLNPIVSPPPPDDDHLSKGTEAPRNSVESEEEESSLPC 120

Query: 121 TPKQKQDGLHFPKGIVQIKTKSGL-KGSAVNSKENLS--GNSPSTKTPTLVARLMGLDLL 180
           TPKQK+DGLHFPKGIVQIKTKSG+ K S +NS +NLS   +SPSTKTPTLVARLMGLDLL
Sbjct: 121 TPKQKEDGLHFPKGIVQIKTKSGITKESEMNSNKNLSAGNDSPSTKTPTLVARLMGLDLL 180

Query: 181 PESNSSPSSTSTPRTASASASASPIYPILQKPKTA-NHLMGSRSLPETPRTSCERK---S 240
           P+S S  ++T TP            Y ++ KPKT  NHL G+RSLPETPRTSCERK    
Sbjct: 181 PQSTSPSTTTRTP------------YSLVGKPKTGKNHLTGTRSLPETPRTSCERKPNVD 240

Query: 241 NYHHRLSLQIPNHYDKENATHHHTPSPTHYAKEIVKQIKETVSRKAGLNDITN---YTRR 300
           NYHHRLSLQIPN +DKENAT   +P+P+HYAKEIVKQIKETVSRK GL+DITN    TRR
Sbjct: 241 NYHHRLSLQIPN-FDKENATPSPSPNPSHYAKEIVKQIKETVSRKGGLSDITNNYYSTRR 300

Query: 301 DQEVLIINQTKPKKPPSSSSSSSVSPRLRVLLDPKNLNKASPKVVEAIKEGGLGMKAKPK 360
           DQ+  +I QTKPKK  S SSSSSVSPRL     PKN NK +PK VEA+KEG LG +    
Sbjct: 301 DQD--MITQTKPKKVLSLSSSSSVSPRL-----PKNPNKPTPK-VEAMKEGVLGQRKPRT 360

Query: 361 QK-VKTKVALKKGSKQEEPFVVPSRITKAAIDSPLKKSKKTPLSNDLLTFSSLPTVVMKK 420
           QK VKTK  LKK +KQEEPFVVPSRITKAAID PLK +KKTPLSN LL+F S+PT++MKK
Sbjct: 361 QKPVKTKAGLKKSNKQEEPFVVPSRITKAAIDGPLKNTKKTPLSNQLLSFGSVPTILMKK 420

Query: 421 DSPFSSPIKPTPIQSPCNQNQAASQTGDKESKRYLQSSNHQPHIPIIHHHHHVIQLP-PN 480
           D PF SPIKP P+QSPCN+NQ A+QT DKESKRYLQSSNHQPHIPIIHHH HVIQLP  N
Sbjct: 421 DPPFPSPIKPFPMQSPCNRNQPANQTVDKESKRYLQSSNHQPHIPIIHHHQHVIQLPLSN 480

Query: 481 NNIITTTSTSAAAAADHRNSHDITAELEYVRQILLRRSTTTSSSVYSTV------NAHYN 540
           +N         AAAAD   +HDI AELEYVRQILLRR  +TS+SVYSTV        H  
Sbjct: 481 SNKDGNNPKQNAAAADCSGNHDIAAELEYVRQILLRR-RSTSTSVYSTVFPENYNTTHNR 540

Query: 541 VSFSHRKLLSHLVEELLKPYLEVRPYRQPAK--ERWPEVVDKLCEKVRKFPRAKCEVLED 600
           VS SHRKLL HLVEELL+PYLEVRPYR  A   E W +VV+KLCEKV++ PRAKCE+LED
Sbjct: 541 VSNSHRKLLCHLVEELLEPYLEVRPYRGAASPGEVWADVVEKLCEKVKRLPRAKCEILED 600

Query: 601 IDGIIEKDLDILGIGFEEEG-EGMVNEIEDWIVEELLNETVRFIEEAKDG 622
           IDGIIEKD+DILGIGFEEEG EG+V EIE+WIVEELLNETVRF+E    G
Sbjct: 601 IDGIIEKDMDILGIGFEEEGEEGIVKEIEEWIVEELLNETVRFVETEMAG 611

BLAST of Tan0022013 vs. ExPASy TrEMBL
Match: A0A6J1JAH9 (uncharacterized protein LOC111482735 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482735 PE=4 SV=1)

HSP 1 Score: 717.6 bits (1851), Expect = 4.4e-203
Identity = 442/654 (67.58%), Postives = 492/654 (75.23%), Query Frame = 0

Query: 1   MGKQRYWSGHGRTATATASATKLKINNNNGFDEANSSFSSSSSSSSSSSSSTSAGCMCAV 60
           MGKQRYWSGHGRTATA   +TK   N  NGFDEA              SSSTSAGCMCAV
Sbjct: 1   MGKQRYWSGHGRTATA---STKTLKNEPNGFDEA--------------SSSTSAGCMCAV 60

Query: 61  FQLFDFHPL--NQHHSP----VSLNHPISPPL--HHHLS----TGTEAPRNSLESDKEES 120
           FQLFDFHPL  +QHH P    VSLN  +SPP     HLS     GTEAPRNS+ES++EES
Sbjct: 61  FQLFDFHPLSHHQHHHPPRSTVSLNPIVSPPPPDDDHLSKVKNLGTEAPRNSVESEEEES 120

Query: 121 PLSSTPKQKQDGLHFPKGIVQIKTKSGL-KGSAVNSKENLS--GNSPSTKTPTLVARLMG 180
            L  TPKQK+DGLHFPKGIVQIKTKSG+ K S +NS +NLS   +SPSTKTPTLVARLMG
Sbjct: 121 SLPCTPKQKEDGLHFPKGIVQIKTKSGITKESEMNSNKNLSAGNDSPSTKTPTLVARLMG 180

Query: 181 LDLLPESNSSPSSTSTPRTASASASASPIYPILQKPKTA-NHLMGSRSLPETPRTSCERK 240
           LDLLP+S S  ++T TP            Y ++ KPKT  NHL G+RSLPETPRTSCERK
Sbjct: 181 LDLLPQSTSPSTTTRTP------------YSLVGKPKTGKNHLTGTRSLPETPRTSCERK 240

Query: 241 ---SNYHHRLSLQIPNHYDKENATHHHTPSPTHYAKEIVKQIKETVSRKAGLNDITN--- 300
               NYHHRLSLQIPN +DKENAT   +P+P+HYAKEIVKQIKETVSRK GL+DITN   
Sbjct: 241 PNVDNYHHRLSLQIPN-FDKENATPSPSPNPSHYAKEIVKQIKETVSRKGGLSDITNNYY 300

Query: 301 YTRRDQEVLIINQTKPKKPPSSSSSSSVSPRLRVLLDPKNLNKASPKVVEAIKEGGLGMK 360
            TRRDQ+  +I QTKPKK  S SSSSSVSPRL     PKN NK +PK VEA+KEG LG +
Sbjct: 301 STRRDQD--MITQTKPKKVLSLSSSSSVSPRL-----PKNPNKPTPK-VEAMKEGVLGQR 360

Query: 361 AKPKQK-VKTKVALKKGSKQEEPFVVPSRITKAAIDSPLKKSKKTPLSNDLLTFSSLPTV 420
               QK VKTK  LKK +KQEEPFVVPSRITKAAID PLK +KKTPLSN LL+F S+PT+
Sbjct: 361 KPRTQKPVKTKAGLKKSNKQEEPFVVPSRITKAAIDGPLKNTKKTPLSNQLLSFGSVPTI 420

Query: 421 VMKKDSPFSSPIKPTPIQSPCNQNQAASQTGDKESKRYLQSSNHQPHIPIIHHHHHVIQL 480
           +MKKD PF SPIKP P+QSPCN+NQ A+QT DKESKRYLQSSNHQPHIPIIHHH HVIQL
Sbjct: 421 LMKKDPPFPSPIKPFPMQSPCNRNQPANQTVDKESKRYLQSSNHQPHIPIIHHHQHVIQL 480

Query: 481 P-PNNNIITTTSTSAAAAADHRNSHDITAELEYVRQILLRRSTTTSSSVYSTV------N 540
           P  N+N         AAAAD   +HDI AELEYVRQILLRR  +TS+SVYSTV       
Sbjct: 481 PLSNSNKDGNNPKQNAAAADCSGNHDIAAELEYVRQILLRR-RSTSTSVYSTVFPENYNT 540

Query: 541 AHYNVSFSHRKLLSHLVEELLKPYLEVRPYRQPAK--ERWPEVVDKLCEKVRKFPRAKCE 600
            H  VS SHRKLL HLVEELL+PYLEVRPYR  A   E W +VV+KLCEKV++ PRAKCE
Sbjct: 541 THNRVSNSHRKLLCHLVEELLEPYLEVRPYRGAASPGEVWADVVEKLCEKVKRLPRAKCE 600

Query: 601 VLEDIDGIIEKDLDILGIGFEEEG-EGMVNEIEDWIVEELLNETVRFIEEAKDG 622
           +LEDIDGIIEKD+DILGIGFEEEG EG+V EIE+WIVEELLNETVRF+E    G
Sbjct: 601 ILEDIDGIIEKDMDILGIGFEEEGEEGIVKEIEEWIVEELLNETVRFVETEMAG 615

BLAST of Tan0022013 vs. ExPASy TrEMBL
Match: A0A6J1JAG9 (uncharacterized protein LOC111482735 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482735 PE=4 SV=1)

HSP 1 Score: 713.8 bits (1841), Expect = 6.4e-202
Identity = 442/664 (66.57%), Postives = 492/664 (74.10%), Query Frame = 0

Query: 1   MGKQRYWSGHGRTATATASATKLKINNNNGFDEANSSFSSSSSSSSSSSSSTSAGCMCAV 60
           MGKQRYWSGHGRTATA   +TK   N  NGFDEA              SSSTSAGCMCAV
Sbjct: 1   MGKQRYWSGHGRTATA---STKTLKNEPNGFDEA--------------SSSTSAGCMCAV 60

Query: 61  FQLFDFHPL--NQHHSP----VSLNHPISPPL--HHHLS--------------TGTEAPR 120
           FQLFDFHPL  +QHH P    VSLN  +SPP     HLS               GTEAPR
Sbjct: 61  FQLFDFHPLSHHQHHHPPRSTVSLNPIVSPPPPDDDHLSKDQNLILSNGSVKNLGTEAPR 120

Query: 121 NSLESDKEESPLSSTPKQKQDGLHFPKGIVQIKTKSGL-KGSAVNSKENLS--GNSPSTK 180
           NS+ES++EES L  TPKQK+DGLHFPKGIVQIKTKSG+ K S +NS +NLS   +SPSTK
Sbjct: 121 NSVESEEEESSLPCTPKQKEDGLHFPKGIVQIKTKSGITKESEMNSNKNLSAGNDSPSTK 180

Query: 181 TPTLVARLMGLDLLPESNSSPSSTSTPRTASASASASPIYPILQKPKTA-NHLMGSRSLP 240
           TPTLVARLMGLDLLP+S S  ++T TP            Y ++ KPKT  NHL G+RSLP
Sbjct: 181 TPTLVARLMGLDLLPQSTSPSTTTRTP------------YSLVGKPKTGKNHLTGTRSLP 240

Query: 241 ETPRTSCERK---SNYHHRLSLQIPNHYDKENATHHHTPSPTHYAKEIVKQIKETVSRKA 300
           ETPRTSCERK    NYHHRLSLQIPN +DKENAT   +P+P+HYAKEIVKQIKETVSRK 
Sbjct: 241 ETPRTSCERKPNVDNYHHRLSLQIPN-FDKENATPSPSPNPSHYAKEIVKQIKETVSRKG 300

Query: 301 GLNDITN---YTRRDQEVLIINQTKPKKPPSSSSSSSVSPRLRVLLDPKNLNKASPKVVE 360
           GL+DITN    TRRDQ+  +I QTKPKK  S SSSSSVSPRL     PKN NK +PK VE
Sbjct: 301 GLSDITNNYYSTRRDQD--MITQTKPKKVLSLSSSSSVSPRL-----PKNPNKPTPK-VE 360

Query: 361 AIKEGGLGMKAKPKQK-VKTKVALKKGSKQEEPFVVPSRITKAAIDSPLKKSKKTPLSND 420
           A+KEG LG +    QK VKTK  LKK +KQEEPFVVPSRITKAAID PLK +KKTPLSN 
Sbjct: 361 AMKEGVLGQRKPRTQKPVKTKAGLKKSNKQEEPFVVPSRITKAAIDGPLKNTKKTPLSNQ 420

Query: 421 LLTFSSLPTVVMKKDSPFSSPIKPTPIQSPCNQNQAASQTGDKESKRYLQSSNHQPHIPI 480
           LL+F S+PT++MKKD PF SPIKP P+QSPCN+NQ A+QT DKESKRYLQSSNHQPHIPI
Sbjct: 421 LLSFGSVPTILMKKDPPFPSPIKPFPMQSPCNRNQPANQTVDKESKRYLQSSNHQPHIPI 480

Query: 481 IHHHHHVIQLP-PNNNIITTTSTSAAAAADHRNSHDITAELEYVRQILLRRSTTTSSSVY 540
           IHHH HVIQLP  N+N         AAAAD   +HDI AELEYVRQILLRR  +TS+SVY
Sbjct: 481 IHHHQHVIQLPLSNSNKDGNNPKQNAAAADCSGNHDIAAELEYVRQILLRR-RSTSTSVY 540

Query: 541 STV------NAHYNVSFSHRKLLSHLVEELLKPYLEVRPYRQPAK--ERWPEVVDKLCEK 600
           STV        H  VS SHRKLL HLVEELL+PYLEVRPYR  A   E W +VV+KLCEK
Sbjct: 541 STVFPENYNTTHNRVSNSHRKLLCHLVEELLEPYLEVRPYRGAASPGEVWADVVEKLCEK 600

Query: 601 VRKFPRAKCEVLEDIDGIIEKDLDILGIGFEEEG-EGMVNEIEDWIVEELLNETVRFIEE 622
           V++ PRAKCE+LEDIDGIIEKD+DILGIGFEEEG EG+V EIE+WIVEELLNETVRF+E 
Sbjct: 601 VKRLPRAKCEILEDIDGIIEKDMDILGIGFEEEGEEGIVKEIEEWIVEELLNETVRFVET 625

BLAST of Tan0022013 vs. ExPASy TrEMBL
Match: A0A6J1E3W8 (uncharacterized protein LOC111430569 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111430569 PE=4 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 5.4e-201
Identity = 438/650 (67.38%), Postives = 491/650 (75.54%), Query Frame = 0

Query: 1   MGKQRYWSGHGRTATATASATKLKINNNNGFDEANSSFSSSSSSSSSSSSSTSAGCMCAV 60
           MGKQRYWSGHGRTATA   +TK   N  NGFDEA              SSSTSAGCMCAV
Sbjct: 1   MGKQRYWSGHGRTATA---STKTLKNQPNGFDEA--------------SSSTSAGCMCAV 60

Query: 61  FQLFDFHPL--NQHHSP----VSLNHPISPPL--HHHLSTGTEAPRNSLESDKEESPLSS 120
           FQLFDFHPL  +QHH P    VSLN  +SPP     HLS GTEAPRNS+ES++EES L  
Sbjct: 61  FQLFDFHPLSHHQHHHPPRSTVSLNPIVSPPPPDDDHLSKGTEAPRNSVESEEEESSLPC 120

Query: 121 TPKQKQDGLHFPKGIVQIKTKSGL-KGSAVNSKENLS--GNSPSTKTPTLVARLMGLDLL 180
           TPKQK+DGLHFPKGIVQIKTKSG+ K S +NS +NLS   +SPSTKTPTLVARLMGLDLL
Sbjct: 121 TPKQKEDGLHFPKGIVQIKTKSGITKESEMNSNKNLSAGNDSPSTKTPTLVARLMGLDLL 180

Query: 181 PESNSSPSSTSTPRTASASASASPIYPILQKPKTA-NHLMGSRSLPETPRTSCERK---S 240
           P+S S  ++T TP            + ++ KPKT  NHL G+RSLPETPRTSCERK    
Sbjct: 181 PQSTSPSTTTRTP------------HSVVGKPKTGKNHLTGTRSLPETPRTSCERKPNVD 240

Query: 241 NYHHRLSLQIPNHYDKENATHHHTPSPTHYAKEIVKQIKETVSRKAGLNDITN---YTRR 300
           NYHHRLSLQIPN +DKENA+    P+P+HYAKEIVKQIKETVSRK GL+DITN    TRR
Sbjct: 241 NYHHRLSLQIPN-FDKENASPSPGPNPSHYAKEIVKQIKETVSRKGGLSDITNNYYSTRR 300

Query: 301 DQEVLIINQTKPKKPPSSSSSSSVSPRLRVLLDPKNLNKASPKVVEAIKEGGLGMKAKPK 360
           DQ+  II QTKPKK  S SSSSSVSPRL     PKN NK +PK VEA+KEG LG +   +
Sbjct: 301 DQD--IITQTKPKKVISLSSSSSVSPRL-----PKNPNKQTPK-VEAMKEGVLGQRKPKR 360

Query: 361 QK-VKTKVALKKGSKQEEPFVVPSRITKAAIDSPLKKSKKTPLSNDLLTFSSLPTVVMKK 420
           QK VKTK  LKK +KQEEPFVVPSRITKAAID PLK +KKTPLSN LL F S+ T+VMKK
Sbjct: 361 QKPVKTKAGLKKSNKQEEPFVVPSRITKAAIDGPLKNTKKTPLSNQLLNFGSVLTIVMKK 420

Query: 421 DSPFSSPIKPTPIQSPCNQNQAASQTGDKESKRYLQSSNHQPHIPIIHHHHHVIQLPPNN 480
           D PF SPIKP P+QSPCN+NQ A+QT DKESKRYLQSSNHQPHI IIHHH HVIQLP +N
Sbjct: 421 DPPFPSPIKPFPMQSPCNRNQPANQTVDKESKRYLQSSNHQPHISIIHHHQHVIQLPLSN 480

Query: 481 NII--TTTSTSAAAAADHRNSHDITAELEYVRQILLRRSTTTSSSVYSTV-NAHYN---- 540
           +         + A+AAD   +HD  AELEYVRQILLRR  +TSSSVYSTV   +YN    
Sbjct: 481 SNKDGNNPKQNTASAADCSGNHDSAAELEYVRQILLRR-RSTSSSVYSTVLPENYNTTPN 540

Query: 541 -VSFSHRKLLSHLVEELLKPYLEVRPYRQPAK--ERWPEVVDKLCEKVRKFPRAKCEVLE 600
            VS SHRKLL HLVEELL+PYLEVRPYR+ A   E W +VV+KLCEKV++ PRAKCE+LE
Sbjct: 541 RVSNSHRKLLCHLVEELLEPYLEVRPYRKAASPGEVWADVVEKLCEKVKRLPRAKCEILE 600

Query: 601 DIDGIIEKDLDILGIGFEEEGEGMVNEIEDWIVEELLNETVRFIEEAKDG 622
           DIDGIIEKD+DILGIGFEEEGEG+V EIE+ IVEELLNETVRF+E    G
Sbjct: 601 DIDGIIEKDMDILGIGFEEEGEGIVKEIEECIVEELLNETVRFVETEMAG 611

BLAST of Tan0022013 vs. ExPASy TrEMBL
Match: A0A6J1E787 (uncharacterized protein LOC111430569 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430569 PE=4 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 3.0e-199
Identity = 438/654 (66.97%), Postives = 491/654 (75.08%), Query Frame = 0

Query: 1   MGKQRYWSGHGRTATATASATKLKINNNNGFDEANSSFSSSSSSSSSSSSSTSAGCMCAV 60
           MGKQRYWSGHGRTATA   +TK   N  NGFDEA              SSSTSAGCMCAV
Sbjct: 1   MGKQRYWSGHGRTATA---STKTLKNQPNGFDEA--------------SSSTSAGCMCAV 60

Query: 61  FQLFDFHPL--NQHHSP----VSLNHPISPPL--HHHLS----TGTEAPRNSLESDKEES 120
           FQLFDFHPL  +QHH P    VSLN  +SPP     HLS     GTEAPRNS+ES++EES
Sbjct: 61  FQLFDFHPLSHHQHHHPPRSTVSLNPIVSPPPPDDDHLSKVKNLGTEAPRNSVESEEEES 120

Query: 121 PLSSTPKQKQDGLHFPKGIVQIKTKSGL-KGSAVNSKENLS--GNSPSTKTPTLVARLMG 180
            L  TPKQK+DGLHFPKGIVQIKTKSG+ K S +NS +NLS   +SPSTKTPTLVARLMG
Sbjct: 121 SLPCTPKQKEDGLHFPKGIVQIKTKSGITKESEMNSNKNLSAGNDSPSTKTPTLVARLMG 180

Query: 181 LDLLPESNSSPSSTSTPRTASASASASPIYPILQKPKTA-NHLMGSRSLPETPRTSCERK 240
           LDLLP+S S  ++T TP            + ++ KPKT  NHL G+RSLPETPRTSCERK
Sbjct: 181 LDLLPQSTSPSTTTRTP------------HSVVGKPKTGKNHLTGTRSLPETPRTSCERK 240

Query: 241 ---SNYHHRLSLQIPNHYDKENATHHHTPSPTHYAKEIVKQIKETVSRKAGLNDITN--- 300
               NYHHRLSLQIPN +DKENA+    P+P+HYAKEIVKQIKETVSRK GL+DITN   
Sbjct: 241 PNVDNYHHRLSLQIPN-FDKENASPSPGPNPSHYAKEIVKQIKETVSRKGGLSDITNNYY 300

Query: 301 YTRRDQEVLIINQTKPKKPPSSSSSSSVSPRLRVLLDPKNLNKASPKVVEAIKEGGLGMK 360
            TRRDQ+  II QTKPKK  S SSSSSVSPRL     PKN NK +PK VEA+KEG LG +
Sbjct: 301 STRRDQD--IITQTKPKKVISLSSSSSVSPRL-----PKNPNKQTPK-VEAMKEGVLGQR 360

Query: 361 AKPKQK-VKTKVALKKGSKQEEPFVVPSRITKAAIDSPLKKSKKTPLSNDLLTFSSLPTV 420
              +QK VKTK  LKK +KQEEPFVVPSRITKAAID PLK +KKTPLSN LL F S+ T+
Sbjct: 361 KPKRQKPVKTKAGLKKSNKQEEPFVVPSRITKAAIDGPLKNTKKTPLSNQLLNFGSVLTI 420

Query: 421 VMKKDSPFSSPIKPTPIQSPCNQNQAASQTGDKESKRYLQSSNHQPHIPIIHHHHHVIQL 480
           VMKKD PF SPIKP P+QSPCN+NQ A+QT DKESKRYLQSSNHQPHI IIHHH HVIQL
Sbjct: 421 VMKKDPPFPSPIKPFPMQSPCNRNQPANQTVDKESKRYLQSSNHQPHISIIHHHQHVIQL 480

Query: 481 PPNNNII--TTTSTSAAAAADHRNSHDITAELEYVRQILLRRSTTTSSSVYSTV-NAHYN 540
           P +N+         + A+AAD   +HD  AELEYVRQILLRR  +TSSSVYSTV   +YN
Sbjct: 481 PLSNSNKDGNNPKQNTASAADCSGNHDSAAELEYVRQILLRR-RSTSSSVYSTVLPENYN 540

Query: 541 -----VSFSHRKLLSHLVEELLKPYLEVRPYRQPAK--ERWPEVVDKLCEKVRKFPRAKC 600
                VS SHRKLL HLVEELL+PYLEVRPYR+ A   E W +VV+KLCEKV++ PRAKC
Sbjct: 541 TTPNRVSNSHRKLLCHLVEELLEPYLEVRPYRKAASPGEVWADVVEKLCEKVKRLPRAKC 600

Query: 601 EVLEDIDGIIEKDLDILGIGFEEEGEGMVNEIEDWIVEELLNETVRFIEEAKDG 622
           E+LEDIDGIIEKD+DILGIGFEEEGEG+V EIE+ IVEELLNETVRF+E    G
Sbjct: 601 EILEDIDGIIEKDMDILGIGFEEEGEGIVKEIEECIVEELLNETVRFVETEMAG 615

BLAST of Tan0022013 vs. TAIR 10
Match: AT5G62170.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G51850.1); Has 381 Blast hits to 359 proteins in 81 species: Archae - 0; Bacteria - 16; Metazoa - 101; Fungi - 21; Plants - 99; Viruses - 3; Other Eukaryotes - 141 (source: NCBI BLink). )

HSP 1 Score: 172.2 bits (435), Expect = 1.3e-42
Identity = 222/711 (31.22%), Postives = 316/711 (44.44%), Query Frame = 0

Query: 1   MGKQRYWSGHGRTATATASATKLKINNNNGFDEANSSFSSSSSSSSSSSSSTSAGCMCAV 60
           MG+   W G G+  +++ S             E +   +     S + +++T+AGCM AV
Sbjct: 1   MGRDWSWLGGGKKKSSSKS------------KEEDIKPTQPPPPSLAGNTATAAGCMSAV 60

Query: 61  FQLFDFHPLNQHHSPVSLNHPISPPLHHHLSTGTEAPRNSLESDKEESPLSSTPKQKQDG 120
           F +FDF      H    +NH      H HL  G +APRNSLES +EE+  S +P +K   
Sbjct: 61  FNIFDF-----QHLQFPINHH-----HLHLPKGVDAPRNSLESTEEET--SFSPTRKDGN 120

Query: 121 LHFPKGIVQIKTKSGLKGSAVNSKENLSGNSPSTKTPTLVARLMGLDLLPE---SNSSPS 180
           L+   GI +IKTK   + S+  S       SPS KTPTLVARLMGLDL+P+   S+ +PS
Sbjct: 121 LNISMGI-KIKTKPQARSSSA-SLTPTETYSPSIKTPTLVARLMGLDLVPDNYRSSPTPS 180

Query: 181 STS--------TPRTASASASASPIYPILQKPKTANHLMGSRSLPETPRTSCERKS---- 240
           S+S        TP T S+ A     Y + +         G+RSLPETPR S  R+S    
Sbjct: 181 SSSSSTLIDLKTP-TRSSHAKKHRHYSLQRNSVDG----GTRSLPETPRISLGRRSVDVN 240

Query: 241 -NYHHRLSLQIPN------------------------HYDKENATHHHTPSPTHYAKEIV 300
              H R SL + +                        H DKEN       SP  YA++IV
Sbjct: 241 CYEHQRSSLHLRDNNINVFPERESGINNVRLTRVKEIHEDKENR------SPREYARQIV 300

Query: 301 KQIKETVSRKAGL-NDITNYTRRDQEV-----------LIINQTK----------PKKPP 360
            Q+KE VSR+  +  DITN   + +EV           +I +             PK  P
Sbjct: 301 MQLKENVSRRRRMGTDITNKETQPREVHESKKASSKTTIITHDVSSSPRLGLTEVPKTKP 360

Query: 361 SSSSSSSVSPRLRVLLDPKNLNKA-------SPKVVEAIKEGGLGMKAKPKQKVKTKVAL 420
           +S  +++V+ ++      K  +K         P+  E  K+     K K  +  K+++  
Sbjct: 361 TSLQTNNVASKILETTAMKVQDKTRLPTVHEEPQGTEKEKQRKSTKKCKKPENFKSRLVK 420

Query: 421 KKGSKQEEPFVVPSRITKA---------AIDSPLKKSKKTPLS-NDLLTFSSLPTVVMKK 480
              S QEEPFV    I  +          I      SKKTPLS N L+ F+S+PT+  K 
Sbjct: 421 PPQSMQEEPFVRSPAINNSNNNNNGHLLLIQGDKSSSKKTPLSINHLINFTSVPTIKKKD 480

Query: 481 DSPF--SSPIKPTPIQSPCNQNQAASQTGDKESKRYLQSSNHQPHIPIIHHHHHVIQLPP 540
             P   SS +K    Q+P N+                 SS+  P  P    HH       
Sbjct: 481 SQPHHKSSNLKLRETQTPRNR----------------ASSSELPSFPSQSQHHIAPIAGG 540

Query: 541 NNNIITTT--------STSAAAAADHRNSHDITAELEYVRQILLRRSTTTSSSVYSTVNA 600
               IT T         T  + A     SH +   + Y  +     ST          N+
Sbjct: 541 ELEYITRTLRRTGIDRDTPISYAKWFSPSHPLDPSIFYFLEHFAVTSTRPR-------NS 600

Query: 601 HYNVSF-SHRKLLSHLVEE----LLKPYLEVRPY------RQPAKERWPEVVDKLCEKVR 612
             N+S   +RKLL HLV+E    +LKP++ ++P+      R     +  E++D+L  ++ 
Sbjct: 601 PENLSLRCNRKLLFHLVDEILADILKPHINLKPWVCHYPIRSQRNLKGSELIDELSRRIE 651

BLAST of Tan0022013 vs. TAIR 10
Match: AT5G51850.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G62170.1); Has 384 Blast hits to 375 proteins in 79 species: Archae - 0; Bacteria - 14; Metazoa - 135; Fungi - 31; Plants - 92; Viruses - 0; Other Eukaryotes - 112 (source: NCBI BLink). )

HSP 1 Score: 88.6 bits (218), Expect = 1.9e-17
Identity = 175/643 (27.22%), Postives = 269/643 (41.84%), Query Frame = 0

Query: 31  FDEANSSFSS--SSSSSSSSSSSTSAGCMCAVFQLFDFHPLNQHHSPVSLNHPISPPLHH 90
           FD+ + S       + SSS S  T+ GCM A + LFD                     HH
Sbjct: 8   FDDGHGSEGGGRGGAFSSSRSKKTANGCMAAFYHLFD--------------------SHH 67

Query: 91  HLSTGTEAPRNSLESDKEESPLSSTPKQKQDGLHFPKGIVQIKTKSGLKGSAVNSKENLS 150
           HL+  + +    L+  +E  P S+T K K+   + P G +++KT +G K S + +    S
Sbjct: 68  HLTIDSPSRSKGLKLMEESLP-STTYKDKEIS-NIPVG-MRVKTDTGTKSSRLRALVTDS 127

Query: 151 G-------NSPSTKTPTLVARLMGLDLLPESNSSPSSTSTPRTASASASASPIYPILQKP 210
                   NSP +KTP LVARLMGLDLLP+      S S   T S+    S  + + +K 
Sbjct: 128 STSSSEICNSPGSKTPNLVARLMGLDLLPDKTDLNHSLSDLHTMSSHHITS--HRLSKK- 187

Query: 211 KTANHLMGSRSLPETPRTSCERKSNYH-HRLSLQIPNHYD------KENATHHHTPSPTH 270
                  G+RSLP +PR S  RKS++  HRLSLQ+    +      KE+    H  SP  
Sbjct: 188 -------GTRSLPVSPRISSARKSDFDIHRLSLQLNREKEFGRSRLKEDQEESH--SPRD 247

Query: 271 YAKEIVKQIKE-TVSRKAGLNDITNYT-----------RRDQEVLII---------NQTK 330
           YA++IVKQIKE  V+R+    DITN             RRD  V            N+  
Sbjct: 248 YARQIVKQIKERVVTRRVVGMDITNSVKNREARPSHELRRDTTVSCSPRTRFSEKENKQS 307

Query: 331 PKKPPSSSSSSSVSPRLR-------VLLDPKNLNKASPKVVEAIKEGGLGMKA--KPKQK 390
               P+SSSSS   P ++       +L + ++ N+   + ++ I    L  KA  + ++ 
Sbjct: 308 TSHKPNSSSSSRPEPIIQKPKPTPVILGEKQSQNRVKQRQLKPI---NLCKKAETETRRP 367

Query: 391 VKTKVALKKGSKQEEPFVVPSRITKAAIDSPLKKSKKTPLSNDLLTFSSLPTVVMKKDSP 450
           +K        +++ E F+  SR  KA     +KK KK P SNDL   S+           
Sbjct: 368 IKPSPTSDIRNRKRETFLSDSRDVKAKPLHKIKKFKKIPKSNDLENISA----------- 427

Query: 451 FSSPIKPTPIQSPCNQNQAASQTGDKESKRYLQSSNHQPHIPIIHHHHHVIQLPPNNNII 510
                     + P  Q     +    E+     SS H+                      
Sbjct: 428 ---------TRPPHQQINERERLISNEAASIRSSSMHK---------------------- 487

Query: 511 TTTSTSAAAAADHR---NSHDITAELEYVRQILLRRSTTTSSSVYSTVNAHYNVSF---- 570
            T   S   A +H+    + +I +E +Y+ +I+      + S     ++    +      
Sbjct: 488 -TEKNSPQVARNHKFDDAATEINSEQDYIIRIMNLAGIKSDSQAMLDLSIFRKLEHFGDY 547

Query: 571 --------SHRKLLSHLVEELLKPYLEVRPYRQPAKERWPEVVDKLCEKVRKFPRAKCEV 613
                    +R+LL  LV E+L   +E    R+    +  E++ +LC  V ++      V
Sbjct: 548 PSGTLALGCNRRLLFDLVNEIL---IETVAKRR-GNYQGSELISELCSAVARYSTKCYPV 565

BLAST of Tan0022013 vs. TAIR 10
Match: AT4G25430.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G51850.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 76.6 bits (187), Expect = 7.7e-14
Identity = 111/381 (29.13%), Postives = 181/381 (47.51%), Query Frame = 0

Query: 40  SSSSSSSSSSSSTSAGCMCAVFQLFDFH----PLNQHHSPVSLNHPISPPLHHHLSTGTE 99
           S+ SS S  +S+ + GC+ A++  F FH    P   HH     +H  S         G  
Sbjct: 11  STCSSKSKKNSNEANGCVTALYHFFHFHHFYFPSRHHH-----HHQPSIDSPSRTRKGLV 70

Query: 100 APRNSLESDKEESPLSSTPKQKQDGLHFPKGIVQIKTKSGLKGSAVNSKENLSGNSPSTK 159
           APRNSL+   EESPLS+  K +++GL+   G      KS L+G  V++  + + N P TK
Sbjct: 71  APRNSLDL-SEESPLSTNYKLEREGLNISVG----GKKSTLRGLLVDTPSH-NCNLPRTK 130

Query: 160 TPTLVARLMGLDLLPESNSSPSSTSTPRTASASASASPIYPILQKPKTANHLMGSRSLPE 219
           TP +VARLMGLDLLP+   +   T +PR              ++  + + +  G+RSLP 
Sbjct: 131 TPNVVARLMGLDLLPD---NLELTRSPRNG------------VRGHRLSGNGSGTRSLPA 190

Query: 220 TPRTSCERKSNYHHRLSLQIPNHYD----------KENATHHHTPSPTHYAKEIVKQIKE 279
           +PR S + +   +HRLSL++    +          KE      +PSP +  ++IVKQ K+
Sbjct: 191 SPRISSDSE---NHRLSLELNRENNKHEEFVRTRLKELKQDEQSPSPRYSGRQIVKQTKK 250

Query: 280 TV-SRKAGLNDITNYTRRDQE-VLIINQTKPKKPPSSSSSSSVSPRLRVLLDPKNLNKAS 339
            V +RK G+ D+TN   + +      N+   K+  +S++ + V   LR    P  +   S
Sbjct: 251 RVTTRKFGM-DVTNLLEKKRAGGAAQNRISQKEKTTSTNPAFV---LRQYQQPATVITLS 310

Query: 340 PKVVEAIKEGGLGMKAKPKQKVKTKVALKKGSKQEE---PFVVPSRITKAAIDSPLKKSK 399
            +  ++++      KA+ K K          +KQ +   P    SR  +  +    K+ K
Sbjct: 311 KENQQSLRPISGWEKAESKSKFSPHPTPNNRNKQRKVLTPVSTHSRSNRCDL-LEKKQCK 357

Query: 400 KTPLSNDLLTFSSLPTVVMKK 402
           K  +++   + +  P   MK+
Sbjct: 371 KIYVTSSAFSATERPRKQMKR 357

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_022984435.11.7e-20468.00uncharacterized protein LOC111482735 isoform X3 [Cucurbita maxima][more]
XP_022984434.19.2e-20367.58uncharacterized protein LOC111482735 isoform X2 [Cucurbita maxima][more]
KAG6576756.13.5e-20267.08hypothetical protein SDJN03_24330, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022984433.11.3e-20166.57uncharacterized protein LOC111482735 isoform X1 [Cucurbita maxima][more]
XP_023553057.16.6e-20167.13uncharacterized protein LOC111810568 isoform X3 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1J8L68.1e-20568.00uncharacterized protein LOC111482735 isoform X3 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1JAH94.4e-20367.58uncharacterized protein LOC111482735 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1JAG96.4e-20266.57uncharacterized protein LOC111482735 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1E3W85.4e-20167.38uncharacterized protein LOC111430569 isoform X3 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1E7873.0e-19966.97uncharacterized protein LOC111430569 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT5G62170.11.3e-4231.22unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT5G51850.11.9e-1727.22unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT4G25430.17.7e-1429.13unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032795DUF3741-associated sequence motifPFAMPF14383VARLMGLcoord: 150..175
e-value: 4.5E-11
score: 42.0
IPR025486Domain of unknown function DUF4378PFAMPF14309DUF4378coord: 506..610
e-value: 1.4E-8
score: 35.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 169..226
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 408..438
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 402..438
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 170..193
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..48
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 77..157
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 289..309
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 135..157
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 10..48
NoneNo IPR availablePANTHERPTHR37751LOW PROTEIN: M-PHASE INDUCER PHOSPHATASE-LIKE PROTEINcoord: 1..614

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0022013.1Tan0022013.1mRNA