Tan0021964 (gene) Snake gourd v1

BLAST of Tan0021964 vs. ExPASy Swiss-Prot
Match: Q43111 (Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 4.0e-32
Identity = 93/257 (36.19%), Postives = 142/257 (55.25%), Query Frame = 0

Query: 1   MDQINALKGYDKVTHLELEDQIPPPSKPNSKIPKQKSLRLTAAISAVVLTTLIIGLFIRV 60
           MD I + KGY KV  LE +          +   K +   +  A+S++VL  +II     V
Sbjct: 1   MDTIKSFKGYGKVNELEQQ----------AYEKKTRKRLIIIAVSSIVLIAVIIAAVAGV 60

Query: 61  YIHNSAGKKSSNS----------ADTIKIVCNVTRYPDSCFTSIFSLNSSPEPDPEVILK 120
            IHN   + S +S          A ++K VC+ TRYP SCF+SI SL  S   DPE++ K
Sbjct: 61  VIHNRNSESSPSSDSVPQTELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFK 120

Query: 121 LSLQVSLIELSNLSRSLETLTGNGDGGAPLKD----CESQIEDAISRVNDSRTEM-EAGE 180
           LSL+V++ ELS+    L     N +  A L+     C S   DA+ R+NDS + +     
Sbjct: 121 LSLRVAIDELSSFPSKLR---ANAEQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAG 180

Query: 181 KTLTERKISDIQTWMSSAMTDQEGCLDGLEEMDSTSFR----EVKMRMTKSIEYVSNSLA 239
           +  +   +S+++TW+S+A+TDQ+ CLD + E++ST+ R    E++  M  S E+ SNSLA
Sbjct: 181 RIASSASVSNVETWLSAALTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLA 240

BLAST of Tan0021964 vs. ExPASy Swiss-Prot
Match: Q43867 (Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 4.8e-30
Identity = 90/257 (35.02%), Postives = 142/257 (55.25%), Query Frame = 0

Query: 1   MDQINALKGYDKVTHLELEDQIPPPSKPNSKIPKQKSLRLTAAISAVVLTTLIIGLFIRV 60
           MD +N+ KGY KV   E +D         +   K +   L  +IS VVL  +II   +  
Sbjct: 1   MDSVNSFKGYGKVD--EAQDL--------ALKKKTRKRLLLLSISVVVLIAVIIAAVVAT 60

Query: 61  YIHNSAGKKSSN------SADTIKIVCNVTRYPDSCFTSIFSLNSSPEPDPEVILKLSLQ 120
            +H +  + + +       + ++K +C+VTR+P+SC +SI  L SS   DPE + KLSL+
Sbjct: 61  VVHKNKNESTPSPPPELTPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLK 120

Query: 121 VSLIELSNLSRSLETLTGNGDG---GAPLKDCESQIEDAISRVND--SRTEMEAGEKTLT 180
           V + EL ++S   E L+   +     + L+ C   IEDA+ R+ND  S  + E  +KTL+
Sbjct: 121 VIIDELDSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLS 180

Query: 181 ERKISDIQTWMSSAMTDQEGCLDGLEEM--------DSTSFREVKMRMTKSIEYVSNSLA 239
             KI D++TW+S+ +TD E C D L+E+        +ST  + +K  M++S E+ SNSLA
Sbjct: 181 SSKIEDLKTWLSATVTDHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLA 240

BLAST of Tan0021964 vs. ExPASy Swiss-Prot
Match: Q9LUL8 (Putative pectinesterase/pectinesterase inhibitor 26 OS=Arabidopsis thaliana OX=3702 GN=PME26 PE=2 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 6.1e-25
Identity = 90/257 (35.02%), Postives = 133/257 (51.75%), Query Frame = 0

Query: 1   MDQINAL-KGYDKVTHLELEDQIPPPSKPNSKIPKQKSLRL-TAAISAVVLTTLIIGLFI 60
           MD + ++ KGY KV   +           +  + ++   RL    IS  VL  +II   +
Sbjct: 1   MDTVKSINKGYGKVDETQ-----------DLALKRKTRKRLYQIGISVAVLVAIIISSTV 60

Query: 61  RVYIHNSAGKK---------SSNSADTIKIVCNVTRYPDSCFTSIFSLNSSPEPDPEVIL 120
            + IH+  G               A ++K VC+VT YP SCF+SI  L  S   DPEVI 
Sbjct: 61  TIAIHSRKGNSPHPTPSSVPELTPAASLKTVCSVTNYPVSCFSSISKLPLSNTTDPEVIF 120

Query: 121 KLSLQVSLIELSN---LSRSLETLTGNGDGGAPLKDCESQIEDAISRVNDSRTEMEA--G 180
           +LSLQV + EL++   L + L   T +    + L  CE  ++ AI RVN++ + ME   G
Sbjct: 121 RLSLQVVIDELNSIVELPKKLAEETDDEGLKSALSVCEHLLDLAIDRVNETVSAMEVVDG 180

Query: 181 EKTLTERKISDIQTWMSSAMTDQEGCLDGLEEMDSTSFR---EVKMRMTKSIEYVSNSLA 239
           +K L    I D+ TW+S+A+T    CLD L+E+  T+     ++K  M  S E+ SNSLA
Sbjct: 181 KKILNAATIDDLLTWLSAAVTYHGTCLDALDEISHTNSAIPLKLKSGMVNSTEFTSNSLA 240

BLAST of Tan0021964 vs. ExPASy Swiss-Prot
Match: Q9SB38 (Pectinesterase inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=PMEI4 PE=2 SV=1)

HSP 1 Score: 74.3 bits (181), Expect = 2.0e-12
Identity = 53/196 (27.04%), Postives = 102/196 (52.04%), Query Frame = 0

Query: 48  VLTTLIIGLFIRVYIHNSAGKKSS--------NSADTIKIVCNVTRYPDSCFTSIFSLNS 107
           +L  +++ L + V+I++S   K++        N    +K  CN T YP  C+  + S +S
Sbjct: 1   MLRFVVLSLTLMVFINSSNFPKTAATPPGTYQNHTTYVKTACNSTTYPTMCYNCLSSYSS 60

Query: 108 SPEPDPEVILKLSLQVSLIELSNLSRSLETLTGNGDGG-----APLKDCESQIEDAISRV 167
           + + DP  +   SL +++    N +  +  L            + LKDC  +++D I  +
Sbjct: 61  TIKSDPIKLCTTSLNLNVKSAKNATLVVSNLLQKAKAAKSHEVSILKDCVDEMKDTIDEL 120

Query: 168 NDSRTEME---AGEKTLTERKISDIQTWMSSAMTDQEGCLDGLEE--MDSTSFREVKMRM 226
             +  EM+    G KT TE  + +++TW+SSA+TD+  C DG EE  ++  + ++VK  +
Sbjct: 121 KQAVAEMKYVRGGGKT-TEEHLKNVKTWVSSALTDEGTCTDGFEEGRVNVETKKKVKKAI 180

BLAST of Tan0021964 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 67.0 bits (162), Expect = 3.3e-10
Identity = 41/168 (24.40%), Postives = 90/168 (53.57%), Query Frame = 0

Query: 64  NSAGKKSSNSADTIKIVCNVTRYPDSCFTSIFSLNSSPEPDPEVILKLSLQVSLIELSNL 123
           +S+   +  + D IK  CN+T Y   C+ S+    S+   +P+ +  ++L ++L    + 
Sbjct: 135 SSSTYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLTLSSAKSA 194

Query: 124 SRSLETLTGNGDGGAPLK-----DCESQIEDAISRVNDSRTEMEA---GEKTLTERKISD 183
           S+ ++ ++ +G G   L+     DC  +I D+++ + DS  E+++    +    E  +SD
Sbjct: 195 SKFVKNIS-HGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKFEMVMSD 254

Query: 184 IQTWMSSAMTDQEGCLDGLEEMDSTSFREVKMRMTKSIEYVSNSLAIV 224
           ++TW+S+A+T+ + C+DG   + +     V+  + +     SN+LA++
Sbjct: 255 VETWVSAALTNDDTCMDGFSLVKTAVKDLVRRHVVEVARLTSNALALI 301

BLAST of Tan0021964 vs. NCBI nr
Match: XP_038895980.1 (pectinesterase 3 [Benincasa hispida])

HSP 1 Score: 361.7 bits (927), Expect = 4.9e-96
Identity = 198/241 (82.16%), Postives = 207/241 (85.89%), Query Frame = 0

Query: 1   MDQINALKGYDKVTHLELEDQIPPPSKPNSKIPKQKSLRLTAAISAVVLTTLIIGLFIRV 60
           MDQI ALKGY KVTHLELEDQ+PPP    SK P  KSLRLTAAISAVVLT LII L I  
Sbjct: 1   MDQIKALKGYGKVTHLELEDQVPPP----SKTPNHKSLRLTAAISAVVLTALIIALIIVA 60

Query: 61  YIHN-SAGKKSSNSADTIKIVCNVTRYPDSCFTSIFSLNSSPEPDPEVILKLSLQVSLIE 120
           YIHN + G KS NSADTIKIVCNVTRYP+SCFTSIFSLNSSPEPDPE+ILKLSLQVSL E
Sbjct: 61  YIHNPTTGNKSPNSADTIKIVCNVTRYPNSCFTSIFSLNSSPEPDPELILKLSLQVSLNE 120

Query: 121 LSNLSRSLETLTGNGDGGAPLKDCESQIEDAISRVNDSRTEME--AGEKTLTERKISDIQ 180
           LSN+SRSL+TL   GDGGA LKDCESQIEDAIS VNDS  EME  AGEKTLTE KI +IQ
Sbjct: 121 LSNMSRSLKTLAAEGDGGAALKDCESQIEDAISEVNDSVAEMESGAGEKTLTESKIGNIQ 180

Query: 181 TWMSSAMTDQEGCLDGLEEMDSTSFREVKMRMTKSIEYVSNSLAIVANIHVILAKFDMPL 239
           TWMSSAMTDQE CL+GLEEMDSTSF+EVK RM KSIEYVSNSLAIVANIHVILA FDMPL
Sbjct: 181 TWMSSAMTDQESCLEGLEEMDSTSFQEVKTRMKKSIEYVSNSLAIVANIHVILANFDMPL 237

BLAST of Tan0021964 vs. NCBI nr
Match: XP_023519544.1 (pectinesterase 3-like [Cucurbita pepo subsp. pepo] >XP_023520180.1 pectinesterase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 360.9 bits (925), Expect = 8.3e-96
Identity = 198/242 (81.82%), Postives = 208/242 (85.95%), Query Frame = 0

Query: 1   MDQINALKGYDKVTHLE--LEDQIPPPSKPNSKIPKQKSLRLTAAISAVVLTTLIIGLFI 60
           MDQINALKGY KVTHLE  LEDQ+PPPSKPN KIP  KSLRLT AISA+VLTTL+IGL I
Sbjct: 1   MDQINALKGYGKVTHLELQLEDQLPPPSKPNFKIPNHKSLRLTIAISALVLTTLLIGLII 60

Query: 61  RVYIHNSAGKKSSNSADTIKIVCNVTRYPDSCFTSIFSLNSSPEPDPEVILKLSLQVSLI 120
              I+NS GKKS NSAD I IVCNVTRYP+SCFTSI SLNSSPEPDPE+ILKLSLQVSL 
Sbjct: 61  AASIYNSTGKKSPNSADAINIVCNVTRYPNSCFTSIISLNSSPEPDPELILKLSLQVSLN 120

Query: 121 ELSNLSRSLETLTGNGDGGAPLKDCESQIEDAISRVNDSRTEME--AGEKTLTERKISDI 180
           ELSNLSRSL+TLT NG GG  LKDCESQIEDAIS VNDS  EME  AGEKTLTE KI +I
Sbjct: 121 ELSNLSRSLKTLTANG-GGEALKDCESQIEDAISLVNDSTAEMESGAGEKTLTESKIGNI 180

Query: 181 QTWMSSAMTDQEGCLDGLEEMDSTSFREVKMRMTKSIEYVSNSLAIVANIHVILAKFDMP 239
           QTWMSSAMTDQ+ CLDGLEEMDSTSFREVK RM KS EYVSN LAIVANIHVIL KF+MP
Sbjct: 181 QTWMSSAMTDQQSCLDGLEEMDSTSFREVKTRMRKSNEYVSNCLAIVANIHVILEKFEMP 240

BLAST of Tan0021964 vs. NCBI nr
Match: XP_022924020.1 (pectinesterase 3 [Cucurbita moschata])

HSP 1 Score: 360.5 bits (924), Expect = 1.1e-95
Identity = 199/242 (82.23%), Postives = 207/242 (85.54%), Query Frame = 0

Query: 1   MDQINALKGYDKVTHLE--LEDQIPPPSKPNSKIPKQKSLRLTAAISAVVLTTLIIGLFI 60
           MDQINALKGY KVTHLE  LEDQ+PPPSKPN KIP  KSLRLT AISAVVLTTL+IGL I
Sbjct: 1   MDQINALKGYGKVTHLELQLEDQLPPPSKPNFKIPNHKSLRLTIAISAVVLTTLLIGLII 60

Query: 61  RVYIHNSAGKKSSNSADTIKIVCNVTRYPDSCFTSIFSLNSSPEPDPEVILKLSLQVSLI 120
              I+NS GKKS NSAD I IVCNVTRYP+SCFTSI SLNSSPEPDPE+ILKLSLQVSL 
Sbjct: 61  AASIYNSTGKKSPNSADAINIVCNVTRYPNSCFTSIISLNSSPEPDPELILKLSLQVSLN 120

Query: 121 ELSNLSRSLETLTGNGDGGAPLKDCESQIEDAISRVNDSRTEM--EAGEKTLTERKISDI 180
           ELSNLSRSL+TLT NG GG  LKDCESQIEDAIS VNDS  EM   AGEKTLTE KI +I
Sbjct: 121 ELSNLSRSLKTLTANG-GGEALKDCESQIEDAISLVNDSTAEMGSGAGEKTLTESKIGNI 180

Query: 181 QTWMSSAMTDQEGCLDGLEEMDSTSFREVKMRMTKSIEYVSNSLAIVANIHVILAKFDMP 239
           QTWMSSAMTDQE CLDGLEEMDSTSFREVK RM KS EYVSN LAIVANIHVIL KF+MP
Sbjct: 181 QTWMSSAMTDQESCLDGLEEMDSTSFREVKTRMRKSNEYVSNCLAIVANIHVILEKFEMP 240

BLAST of Tan0021964 vs. NCBI nr
Match: KAG6584449.1 (Pectinesterase 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020040.1 Pectinesterase 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 359.4 bits (921), Expect = 2.4e-95
Identity = 198/242 (81.82%), Postives = 207/242 (85.54%), Query Frame = 0

Query: 1   MDQINALKGYDKVTHLE--LEDQIPPPSKPNSKIPKQKSLRLTAAISAVVLTTLIIGLFI 60
           MDQINALKGY KVTHLE  LEDQ+PPPSKPN KIP  KSLRLT AISAVVLTTL+IGL I
Sbjct: 1   MDQINALKGYGKVTHLELQLEDQLPPPSKPNFKIPNHKSLRLTIAISAVVLTTLLIGLII 60

Query: 61  RVYIHNSAGKKSSNSADTIKIVCNVTRYPDSCFTSIFSLNSSPEPDPEVILKLSLQVSLI 120
              I+NS GKKS NSAD I IVCNVTRYP+SCFTSI SLNSSPEPDPE+ILKLSLQVSL 
Sbjct: 61  AASIYNSTGKKSPNSADAINIVCNVTRYPNSCFTSIISLNSSPEPDPELILKLSLQVSLN 120

Query: 121 ELSNLSRSLETLTGNGDGGAPLKDCESQIEDAISRVNDSRTEM--EAGEKTLTERKISDI 180
           ELSNLSRSL+TLT NG GG  LKDCESQIEDAIS VNDS  EM   AGEKTLTE KI +I
Sbjct: 121 ELSNLSRSLKTLTANG-GGEALKDCESQIEDAISLVNDSTAEMGSGAGEKTLTESKIGNI 180

Query: 181 QTWMSSAMTDQEGCLDGLEEMDSTSFREVKMRMTKSIEYVSNSLAIVANIHVILAKFDMP 239
           QTWMSSAMTDQE CLDGLEEMDSTSFREVK RM KS EY+SN LAIVANIHVIL KF+MP
Sbjct: 181 QTWMSSAMTDQESCLDGLEEMDSTSFREVKTRMRKSNEYLSNCLAIVANIHVILEKFEMP 240

BLAST of Tan0021964 vs. NCBI nr
Match: XP_023001663.1 (pectinesterase 3 [Cucurbita maxima])

HSP 1 Score: 358.2 bits (918), Expect = 5.4e-95
Identity = 198/245 (80.82%), Postives = 207/245 (84.49%), Query Frame = 0

Query: 1   MDQINALKGYDKVTHLE--LEDQIPPPSKPNSKIPKQKSLRLTAAISAVVLTTLIIGLFI 60
           MDQINALKGY KVTHLE  LEDQ+PPPSKPN KIP  KSLRLT AISAVVLTTL+IG  I
Sbjct: 1   MDQINALKGYGKVTHLELQLEDQLPPPSKPNFKIPNHKSLRLTIAISAVVLTTLLIGFII 60

Query: 61  RVYIHNSAGKKSSNSADTIKIVCNVTRYPDSCFTSIFSLNSSPEPDPEVILKLSLQVSLI 120
              I+NS GK S NSAD I IVCNVTRYP+SCFTSI SLNSSPEPDPE+ILKLSLQVSL 
Sbjct: 61  AASIYNSTGKNSPNSADAINIVCNVTRYPNSCFTSIISLNSSPEPDPELILKLSLQVSLN 120

Query: 121 ELSNLSRSLETLTGNGD---GGAPLKDCESQIEDAISRVNDSRTEME--AGEKTLTERKI 180
           ELSNLSRSL+TLT +GD   GG  LKDCESQIEDAIS VNDS  EME  AGEKTLTE KI
Sbjct: 121 ELSNLSRSLKTLTADGDGNGGGEALKDCESQIEDAISLVNDSTAEMESGAGEKTLTESKI 180

Query: 181 SDIQTWMSSAMTDQEGCLDGLEEMDSTSFREVKMRMTKSIEYVSNSLAIVANIHVILAKF 239
            +IQTWMSSAMT QE CLDGLEEMDSTSFREVKMRM KS EYVSN LAIVANIHVIL KF
Sbjct: 181 GNIQTWMSSAMTYQESCLDGLEEMDSTSFREVKMRMRKSNEYVSNCLAIVANIHVILEKF 240

BLAST of Tan0021964 vs. ExPASy TrEMBL
Match: A0A6J1EDL6 (pectinesterase 3 OS=Cucurbita moschata OX=3662 GN=LOC111431569 PE=4 SV=1)

HSP 1 Score: 360.5 bits (924), Expect = 5.3e-96
Identity = 199/242 (82.23%), Postives = 207/242 (85.54%), Query Frame = 0

Query: 1   MDQINALKGYDKVTHLE--LEDQIPPPSKPNSKIPKQKSLRLTAAISAVVLTTLIIGLFI 60
           MDQINALKGY KVTHLE  LEDQ+PPPSKPN KIP  KSLRLT AISAVVLTTL+IGL I
Sbjct: 1   MDQINALKGYGKVTHLELQLEDQLPPPSKPNFKIPNHKSLRLTIAISAVVLTTLLIGLII 60

Query: 61  RVYIHNSAGKKSSNSADTIKIVCNVTRYPDSCFTSIFSLNSSPEPDPEVILKLSLQVSLI 120
              I+NS GKKS NSAD I IVCNVTRYP+SCFTSI SLNSSPEPDPE+ILKLSLQVSL 
Sbjct: 61  AASIYNSTGKKSPNSADAINIVCNVTRYPNSCFTSIISLNSSPEPDPELILKLSLQVSLN 120

Query: 121 ELSNLSRSLETLTGNGDGGAPLKDCESQIEDAISRVNDSRTEM--EAGEKTLTERKISDI 180
           ELSNLSRSL+TLT NG GG  LKDCESQIEDAIS VNDS  EM   AGEKTLTE KI +I
Sbjct: 121 ELSNLSRSLKTLTANG-GGEALKDCESQIEDAISLVNDSTAEMGSGAGEKTLTESKIGNI 180

Query: 181 QTWMSSAMTDQEGCLDGLEEMDSTSFREVKMRMTKSIEYVSNSLAIVANIHVILAKFDMP 239
           QTWMSSAMTDQE CLDGLEEMDSTSFREVK RM KS EYVSN LAIVANIHVIL KF+MP
Sbjct: 181 QTWMSSAMTDQESCLDGLEEMDSTSFREVKTRMRKSNEYVSNCLAIVANIHVILEKFEMP 240

BLAST of Tan0021964 vs. ExPASy TrEMBL
Match: A0A6J1KR63 (pectinesterase 3 OS=Cucurbita maxima OX=3661 GN=LOC111495737 PE=4 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 2.6e-95
Identity = 198/245 (80.82%), Postives = 207/245 (84.49%), Query Frame = 0

Query: 1   MDQINALKGYDKVTHLE--LEDQIPPPSKPNSKIPKQKSLRLTAAISAVVLTTLIIGLFI 60
           MDQINALKGY KVTHLE  LEDQ+PPPSKPN KIP  KSLRLT AISAVVLTTL+IG  I
Sbjct: 1   MDQINALKGYGKVTHLELQLEDQLPPPSKPNFKIPNHKSLRLTIAISAVVLTTLLIGFII 60

Query: 61  RVYIHNSAGKKSSNSADTIKIVCNVTRYPDSCFTSIFSLNSSPEPDPEVILKLSLQVSLI 120
              I+NS GK S NSAD I IVCNVTRYP+SCFTSI SLNSSPEPDPE+ILKLSLQVSL 
Sbjct: 61  AASIYNSTGKNSPNSADAINIVCNVTRYPNSCFTSIISLNSSPEPDPELILKLSLQVSLN 120

Query: 121 ELSNLSRSLETLTGNGD---GGAPLKDCESQIEDAISRVNDSRTEME--AGEKTLTERKI 180
           ELSNLSRSL+TLT +GD   GG  LKDCESQIEDAIS VNDS  EME  AGEKTLTE KI
Sbjct: 121 ELSNLSRSLKTLTADGDGNGGGEALKDCESQIEDAISLVNDSTAEMESGAGEKTLTESKI 180

Query: 181 SDIQTWMSSAMTDQEGCLDGLEEMDSTSFREVKMRMTKSIEYVSNSLAIVANIHVILAKF 239
            +IQTWMSSAMT QE CLDGLEEMDSTSFREVKMRM KS EYVSN LAIVANIHVIL KF
Sbjct: 181 GNIQTWMSSAMTYQESCLDGLEEMDSTSFREVKMRMRKSNEYVSNCLAIVANIHVILEKF 240

BLAST of Tan0021964 vs. ExPASy TrEMBL
Match: A0A6J1C685 (pectinesterase 3 OS=Momordica charantia OX=3673 GN=LOC111008783 PE=4 SV=1)

HSP 1 Score: 341.3 bits (874), Expect = 3.3e-90
Identity = 191/249 (76.71%), Postives = 205/249 (82.33%), Query Frame = 0

Query: 1   MDQINALKGYDKVTHLELEDQIPPPSKPNSKIPKQKSLRLTAAISAVVLTTLIIGLFIRV 60
           MDQINALKGY KVTHLEL DQIPPPSKPN +  KQKSL +   I+AVVL+TL++G     
Sbjct: 1   MDQINALKGYGKVTHLELGDQIPPPSKPNFRSRKQKSLLI---ITAVVLSTLVVG----A 60

Query: 61  YIHNSAGKKS-------SNSADTI-KIVCNVTRYPDSCFTSIFSLNSSPEPDPEVILKLS 120
           YIHNS G KS       SNSAD+I KIVCNVTRYPDSCFTSI SLN SPEPDPEVILKLS
Sbjct: 61  YIHNSTGDKSSKSPSLNSNSADSIVKIVCNVTRYPDSCFTSIISLNGSPEPDPEVILKLS 120

Query: 121 LQVSLIELSNLSRSLETLTGNGDGGAPLKDCESQIEDAISRVNDSRTEME---AGEKTLT 180
           LQVSL ELSNLS SL+ L+ NGDGG  LKDCESQIEDAI RVN+S+ EME   AGEKTLT
Sbjct: 121 LQVSLDELSNLSGSLKNLSANGDGGGALKDCESQIEDAIERVNESKAEMEADAAGEKTLT 180

Query: 181 ERKISDIQTWMSSAMTDQEGCLDGLEEMDSTSFREVKMRMTKSIEYVSNSLAIVANIHVI 239
           E KI DIQTWMSSAMTDQE CLDGLEEMDSTSF EVK RM KS++YVSNSLAIVANIHVI
Sbjct: 181 ESKIGDIQTWMSSAMTDQESCLDGLEEMDSTSFLEVKTRMRKSVQYVSNSLAIVANIHVI 240

BLAST of Tan0021964 vs. ExPASy TrEMBL
Match: A0A0A0LVF4 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G033000 PE=4 SV=1)

HSP 1 Score: 316.6 bits (810), Expect = 8.7e-83
Identity = 179/252 (71.03%), Postives = 204/252 (80.95%), Query Frame = 0

Query: 1   MDQINALKGYDKVTH--LELEDQI-PPPSKPNSKIPKQKS----LRLTAAISAVVLTTLI 60
           MDQINALKGY K+TH  L+LE QI PPPSKPNSK P   +    LR  AAISA++LT LI
Sbjct: 1   MDQINALKGYGKLTHLNLDLEHQIPPPPSKPNSKFPTNHNFSLLLRFAAAISALLLTALI 60

Query: 61  IGLFIRVYIHNSA---GKKSSNSADTIKIVCNVTRYPDSCFTSIFSLNSSPEPDPEVILK 120
           I L + VYIHNS       S+N+A TI IVCNVTRYP+SCFTSIFSLNSSP+PDPE+IL 
Sbjct: 61  ISLIVGVYIHNSTPDNKSSSNNAAHTISIVCNVTRYPNSCFTSIFSLNSSPQPDPELILN 120

Query: 121 LSLQVSLIELSNLSRSLETLTGNGDGG--APLKDCESQIEDAISRVNDSRTEME--AGEK 180
           LSLQVSL ELSN+SR L+++ G GDGG  A LKDC+SQIEDAIS+VNDS  EM   +GEK
Sbjct: 121 LSLQVSLNELSNMSRWLKSVGGEGDGGAAAALKDCQSQIEDAISQVNDSVAEMRGGSGEK 180

Query: 181 TLTERKISDIQTWMSSAMTDQEGCLDGLEEMDSTSFREVKMRMTKSIEYVSNSLAIVANI 239
           TLTE KI +IQTWMSSAMT++E CL+G+EEMD+TSF EVK RM KSIEYVSNSLAIVANI
Sbjct: 181 TLTESKIGNIQTWMSSAMTNEESCLEGVEEMDATSFEEVKRRMKKSIEYVSNSLAIVANI 240

BLAST of Tan0021964 vs. ExPASy TrEMBL
Match: A0A5A7T3U1 (Pectinesterase 3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00720 PE=4 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 1.1e-80
Identity = 176/252 (69.84%), Postives = 200/252 (79.37%), Query Frame = 0

Query: 1   MDQINALKGYDKVTH--LELEDQI-PPPSKPNSKIPKQKS----LRLTAAISAVVLTTLI 60
           MDQINALKGY K+TH  L+LE QI PPPSKPNSK P   +    LR  AAISA++LT LI
Sbjct: 1   MDQINALKGYGKLTHHNLDLEHQIPPPPSKPNSKFPNNHNYSLPLRFAAAISALLLTALI 60

Query: 61  IGLFIRVYIHNSA---GKKSSNSADTIKIVCNVTRYPDSCFTSIFSLNSSPEPDPEVILK 120
           I L + VY HNS       S+N+A TI I+CNVTRYP+SCFTSI SLNSSP+PDPE+IL 
Sbjct: 61  ISLIVGVYTHNSTPDNKSSSNNAAHTISIICNVTRYPNSCFTSISSLNSSPQPDPELILN 120

Query: 121 LSLQVSLIELSNLSRSLETLTGNGDGG--APLKDCESQIEDAISRVNDSRTEME--AGEK 180
           LSLQVSL ELSN+SR ++TL   GDGG  A LKDC+SQIEDAIS+VN+S  EM   +GE 
Sbjct: 121 LSLQVSLNELSNISRWVKTLGAEGDGGAAAALKDCQSQIEDAISQVNESVAEMRGGSGEM 180

Query: 181 TLTERKISDIQTWMSSAMTDQEGCLDGLEEMDSTSFREVKMRMTKSIEYVSNSLAIVANI 239
           TLTE KI +IQTWMSSAMT++E CLDG+EEMDSTSF EVK RM KSIEYVSNSLAIVANI
Sbjct: 181 TLTESKIGNIQTWMSSAMTNEESCLDGVEEMDSTSFEEVKRRMKKSIEYVSNSLAIVANI 240

BLAST of Tan0021964 vs. TAIR 10
Match: AT3G47670.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 134.8 bits (338), Expect = 9.1e-32
Identity = 95/245 (38.78%), Postives = 147/245 (60.00%), Query Frame = 0

Query: 1   MDQINALKGYDKVTHLELEDQI-----PPPSKPNSKIPKQKSLRLTAAISAVVLTTLIIG 60
           M+ IN LKGYDKV+  + E+QI      P  KP+S    +K+L  T ++ +++L   ++ 
Sbjct: 37  MESINTLKGYDKVS--DQENQIHHHHHSPLPKPSSF--SRKTLIATGSVVSLLLILSVVA 96

Query: 61  LFIRVYIHNSAGKKSSNSADTIKIVCNVTRYPDSCFTSI-FSLNSSPEP-DPEVILKLSL 120
           L    + H+       +SA ++K +C+VTRYP++CF S+  SLN S    +PE IL+LSL
Sbjct: 97  LTAGAFTHSPPHHPPISSA-SLKSLCSVTRYPETCFNSLSSSLNESDSKLNPESILELSL 156

Query: 121 QVSLIELSNLSRSLETLTGNGDGGAPLKDCESQIEDAISRVNDSRTEME-----AGEKTL 180
           +V+  E+SNLS S  ++    +  A + DC     DA+S++NDS TE+E      G   L
Sbjct: 157 RVAAKEISNLSISFRSINDMPEDAA-VGDCVKLYTDALSQLNDSITEIERKKKKGGNNWL 216

Query: 181 TERKISDIQTWMSSAMTDQEGCLDGLEEMDSTSFREVKMRMTKSIEYVSNSLAIVANIHV 234
           TE  + D++TW+S+AMTD E C DG+EEM +    E+K +M  + + +S SLAIV+ +  
Sbjct: 217 TEEVVGDVKTWISAAMTDGETCSDGIEEMGTIVGNEIKKKMEMANQMMSISLAIVSQMKK 275

BLAST of Tan0021964 vs. TAIR 10
Match: AT1G53840.1 (pectin methylesterase 1 )

HSP 1 Score: 132.9 bits (333), Expect = 3.4e-31
Identity = 90/257 (35.02%), Postives = 142/257 (55.25%), Query Frame = 0

Query: 1   MDQINALKGYDKVTHLELEDQIPPPSKPNSKIPKQKSLRLTAAISAVVLTTLIIGLFIRV 60
           MD +N+ KGY KV   E +D         +   K +   L  +IS VVL  +II   +  
Sbjct: 1   MDSVNSFKGYGKVD--EAQDL--------ALKKKTRKRLLLLSISVVVLIAVIIAAVVAT 60

Query: 61  YIHNSAGKKSSN------SADTIKIVCNVTRYPDSCFTSIFSLNSSPEPDPEVILKLSLQ 120
            +H +  + + +       + ++K +C+VTR+P+SC +SI  L SS   DPE + KLSL+
Sbjct: 61  VVHKNKNESTPSPPPELTPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLK 120

Query: 121 VSLIELSNLSRSLETLTGNGDG---GAPLKDCESQIEDAISRVND--SRTEMEAGEKTLT 180
           V + EL ++S   E L+   +     + L+ C   IEDA+ R+ND  S  + E  +KTL+
Sbjct: 121 VIIDELDSISDLPEKLSKETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLS 180

Query: 181 ERKISDIQTWMSSAMTDQEGCLDGLEEM--------DSTSFREVKMRMTKSIEYVSNSLA 239
             KI D++TW+S+ +TD E C D L+E+        +ST  + +K  M++S E+ SNSLA
Sbjct: 181 SSKIEDLKTWLSATVTDHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLA 240

BLAST of Tan0021964 vs. TAIR 10
Match: AT3G14300.1 (pectinesterase family protein )

HSP 1 Score: 115.9 bits (289), Expect = 4.4e-26
Identity = 90/257 (35.02%), Postives = 133/257 (51.75%), Query Frame = 0

Query: 1   MDQINAL-KGYDKVTHLELEDQIPPPSKPNSKIPKQKSLRL-TAAISAVVLTTLIIGLFI 60
           MD + ++ KGY KV   +           +  + ++   RL    IS  VL  +II   +
Sbjct: 1   MDTVKSINKGYGKVDETQ-----------DLALKRKTRKRLYQIGISVAVLVAIIISSTV 60

Query: 61  RVYIHNSAGKK---------SSNSADTIKIVCNVTRYPDSCFTSIFSLNSSPEPDPEVIL 120
            + IH+  G               A ++K VC+VT YP SCF+SI  L  S   DPEVI 
Sbjct: 61  TIAIHSRKGNSPHPTPSSVPELTPAASLKTVCSVTNYPVSCFSSISKLPLSNTTDPEVIF 120

Query: 121 KLSLQVSLIELSN---LSRSLETLTGNGDGGAPLKDCESQIEDAISRVNDSRTEMEA--G 180
           +LSLQV + EL++   L + L   T +    + L  CE  ++ AI RVN++ + ME   G
Sbjct: 121 RLSLQVVIDELNSIVELPKKLAEETDDEGLKSALSVCEHLLDLAIDRVNETVSAMEVVDG 180

Query: 181 EKTLTERKISDIQTWMSSAMTDQEGCLDGLEEMDSTSFR---EVKMRMTKSIEYVSNSLA 239
           +K L    I D+ TW+S+A+T    CLD L+E+  T+     ++K  M  S E+ SNSLA
Sbjct: 181 KKILNAATIDDLLTWLSAAVTYHGTCLDALDEISHTNSAIPLKLKSGMVNSTEFTSNSLA 240

BLAST of Tan0021964 vs. TAIR 10
Match: AT2G01610.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 91.3 bits (225), Expect = 1.1e-18
Identity = 64/194 (32.99%), Postives = 98/194 (50.52%), Query Frame = 0

Query: 52  LIIGLFIRVYIHNSAGKKSSNSADTIKIVCNVTRYPDSCFTSIFSLNSSPEPDPEVILKL 111
           LI G    V +       ++N  D I+  CN T YPD CFTS+    S+ +  P  + KL
Sbjct: 20  LIFGSISAVRLLPRPNTTTTNDLDFIRTSCNATLYPDVCFTSLSGYASAVQDSPARLAKL 79

Query: 112 SLQVSLIE-------LSNLSRSLETLTGNG--DGGAPLKDCESQIEDAISRVNDSRTEME 171
           ++ VSL +       LS LSRS    +G+G     A ++DC S +EDA+  +  S  ++ 
Sbjct: 80  AIGVSLSQAKSTAAFLSKLSRSAAKYSGDGHQTASAVIRDCVSNVEDAVDEMRGSLRQLR 139

Query: 172 ----AGEKTLTERKI-------SDIQTWMSSAMTDQEGCLDGLEEMDSTSFREVKM--RM 224
                G  T   R +       S++QTWMS+A+TD++ C DG E+MD     +  +  R+
Sbjct: 140 DMNGRGGGTAARRSVETFRFQMSNVQTWMSAALTDEDTCTDGFEDMDEGGLIKTTVCDRL 199

BLAST of Tan0021964 vs. TAIR 10
Match: AT1G23205.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 80.9 bits (198), Expect = 1.6e-15
Identity = 52/169 (30.77%), Postives = 90/169 (53.25%), Query Frame = 0

Query: 71  SNSADTIKIVCNVTRYPDSCFTSIFSLNSSPEPDPEVILKLSLQVSLIELSNLSRSLETL 130
           S+ +D I+  CN T YPD CF+S+ S +SS   DP ++ + ++ V+L +  +L+  L  +
Sbjct: 28  SDDSDFIRTSCNTTLYPDLCFSSLSSFSSSVHNDPALLARAAISVTLTKTLDLASYLANI 87

Query: 131 T-----GNGDG------GAPLKDCESQIEDAISRVNDSRTEM----EAGEKTLTERKISD 190
           T      N DG       A   DC   ++DA+  +  S  +M      G       ++S+
Sbjct: 88  TTLQPESNEDGAHHPTAAAVFHDCFDNLKDAVEEMKGSMKQMRELVSTGSLESFRFQMSN 147

Query: 191 IQTWMSSAMTDQEGCLDGLEEM-DSTSFREVKMRMTKSIEYVSNSLAIV 224
           +QTW+S+A+TD+E C DG +++ D     ++  R+    +  SN+LA+V
Sbjct: 148 VQTWLSAALTDEETCTDGFKDIHDEPRKDDICARVDDVKKMTSNALALV 196