Tan0021958 (gene) Snake gourd v1

Overview


Name	Tan0021958
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Pectinesterase inhibitor domain containing protein
Location	LG02: 90560999 .. 90561556 (+)
RNA-Seq Expression	Tan0021958
Synteny	Tan0021958

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTTGATCAGAAGTCATTATTCTGCTTATTGTTGTTTGTTGTTGGTGGCAATGGCGGGTATGTGGCAGAGTGGGGAGGGGCAGGAGCAGGAACGAGAGCGAGAGCTTCTGAGTATGAATGATCTGAATGTGAATTTGGGGATATTGGACATCTGCGGAAATGCAGATTATCCGGCGTTGTGCCGATCTGTAGTGAAGGGTGAGAGAAACCCATCTGTGGCGATCGAATCAGCGATAAGGCAATTGATGTTTCAGACGGACATGGCGAAGGGTGTGGCACAACGTCGCAAGAAGTCAGAGGGGGCCATGGATGTATGCTTCGAGAATTACGACGACGCGTTCAGCAACCTGGAGACTTGCCTTCAAAGCCTGAAAAGCCGAGACAAGCCAGGCCTCAACATCAACCTCTCTGCAGCTTTGACCGATTACGTGACATGCGACGATGCAGTAGCCGAAGGAGGTCCTTCTTCTTCCGTTACGCGAACAAACGTTATGCTGCGCCAAATGGCCAGCAACTGTTTGTATTTGTCTAGTTTGATTCGCCTTCGCCAATAA

mRNA sequence

ATGGCTTTGATCAGAAGTCATTATTCTGCTTATTGTTGTTTGTTGTTGGTGGCAATGGCGGGTATGTGGCAGAGTGGGGAGGGGCAGGAGCAGGAACGAGAGCGAGAGCTTCTGAGTATGAATGATCTGAATGTGAATTTGGGGATATTGGACATCTGCGGAAATGCAGATTATCCGGCGTTGTGCCGATCTGTAGTGAAGGGTGAGAGAAACCCATCTGTGGCGATCGAATCAGCGATAAGGCAATTGATGTTTCAGACGGACATGGCGAAGGGTGTGGCACAACGTCGCAAGAAGTCAGAGGGGGCCATGGATGTATGCTTCGAGAATTACGACGACGCGTTCAGCAACCTGGAGACTTGCCTTCAAAGCCTGAAAAGCCGAGACAAGCCAGGCCTCAACATCAACCTCTCTGCAGCTTTGACCGATTACGTGACATGCGACGATGCAGTAGCCGAAGGAGGTCCTTCTTCTTCCGTTACGCGAACAAACGTTATGCTGCGCCAAATGGCCAGCAACTGTTTGTATTTGTCTAGTTTGATTCGCCTTCGCCAATAA

Coding sequence (CDS)

ATGGCTTTGATCAGAAGTCATTATTCTGCTTATTGTTGTTTGTTGTTGGTGGCAATGGCGGGTATGTGGCAGAGTGGGGAGGGGCAGGAGCAGGAACGAGAGCGAGAGCTTCTGAGTATGAATGATCTGAATGTGAATTTGGGGATATTGGACATCTGCGGAAATGCAGATTATCCGGCGTTGTGCCGATCTGTAGTGAAGGGTGAGAGAAACCCATCTGTGGCGATCGAATCAGCGATAAGGCAATTGATGTTTCAGACGGACATGGCGAAGGGTGTGGCACAACGTCGCAAGAAGTCAGAGGGGGCCATGGATGTATGCTTCGAGAATTACGACGACGCGTTCAGCAACCTGGAGACTTGCCTTCAAAGCCTGAAAAGCCGAGACAAGCCAGGCCTCAACATCAACCTCTCTGCAGCTTTGACCGATTACGTGACATGCGACGATGCAGTAGCCGAAGGAGGTCCTTCTTCTTCCGTTACGCGAACAAACGTTATGCTGCGCCAAATGGCCAGCAACTGTTTGTATTTGTCTAGTTTGATTCGCCTTCGCCAATAA

Protein sequence

MALIRSHYSAYCCLLLVAMAGMWQSGEGQEQERERELLSMNDLNVNLGILDICGNADYPALCRSVVKGERNPSVAIESAIRQLMFQTDMAKGVAQRRKKSEGAMDVCFENYDDAFSNLETCLQSLKSRDKPGLNINLSAALTDYVTCDDAVAEGGPSSSVTRTNVMLRQMASNCLYLSSLIRLRQ

Homology

[Close]

BLAST of Tan0021958 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 1.4e-05
Identity = 31/83 (37.35%), Postives = 43/83 (51.81%), Query Frame = 0


Query: 91  KGVAQRRKKSEGAMDV------CFENYDDAFSNLETCLQSLKSRDKPGLNINLSAALTDY 150
           KG+  +  K +   D+      C   Y+DA  +L T L ++KSRD  G NINLSAAL   
Sbjct: 78  KGIVAQDLKDQRYQDIVEDLKLCLGFYNDANDDLTTALANIKSRDYQGANINLSAALDVP 137

Query: 151 VTCDDAVAEGGPSSSVTRTNVML 168
             C+D   E   +S +T  N +L
Sbjct: 138 GNCEDDFKEAKKTSPITNENSIL 160

[Close]

BLAST of Tan0021958 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 48.5 bits (114), Expect = 9.3e-05
Identity = 37/141 (26.24%), Postives = 61/141 (43.26%), Query Frame = 0


Query: 52  ICGNADYPALCRSVVKGERNPSVAIESAIRQLMF---QTDMAKGVAQ--------RRKKS 111
           ICG A   + C S +K     S A    +  + F   QT  ++G  +             
Sbjct: 33  ICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGSAQTSASEGFRKIQSLVKTATNPTM 92

Query: 112 EGAMDVCFENYDDAFSNLETCLQSLKSRDKPGLNINLSAALTDYVTCDDAVAEGGPSSSV 171
           + A   C ++Y  A S+L    QSL S D  GLNI +SAA+    TC+  +A+     S 
Sbjct: 93  KKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTCEQDMADFKVDPSA 152

Query: 172 TRTNVMLRQMASNCLYLSSLI 182
            + +   + +    L +S+++
Sbjct: 153 VKNSGDFQNICGIVLVISNMM 173

[Close]

BLAST of Tan0021958 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 7.9e-04
Identity = 27/76 (35.53%), Postives = 42/76 (55.26%), Query Frame = 0


Query: 107 CFENYDDAFSNLETCLQSLKSRDKPGLNINLSAALTDYVTCDDAVAE-GGPSSSVTRTNV 166
           C   Y DA S+++  +   KS+D    N+ +SAAL D VTC+D   E  G +S VT+ N 
Sbjct: 93  CVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIASPVTKENK 152

Query: 167 MLRQMASNCLYLSSLI 182
              Q+ +  L ++ L+
Sbjct: 153 DYVQLTAISLAITKLL 168

[Close]

BLAST of Tan0021958 vs. NCBI nr
Match: XP_023536926.1 (uncharacterized protein LOC111798158 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 231.5 bits (589), Expect = 5.9e-57
Identity = 125/181 (69.06%), Postives = 147/181 (81.22%), Query Frame = 0


Query: 5   RSHYSAYC-CLLLVAMAGMWQSGEGQEQERERELLSMNDLNVNLGILDICGNADYPALCR 64
           RSH+  +C CLLLVAMAGMW+ G+GQ QE+ERELLS+N L+ N+G  D+CG A+YP LCR
Sbjct: 4   RSHF--WCGCLLLVAMAGMWEGGQGQGQEQERELLSLN-LDPNMG--DLCGKAEYPVLCR 63

Query: 65  SVVKGERNPSVAIESAIRQLMFQTDMAKGVAQRRKKSEGAMDVCFENYDDAFSNLETCLQ 124
           SVVKG   PSVA+ESAIRQLM QT MA+  A+R +KS G M+VC E Y DAF NL+TCL 
Sbjct: 64  SVVKGLSKPSVALESAIRQLMLQTKMARTTAKRLRKSPG-MEVCSETYKDAFGNLKTCLS 123

Query: 125 SLKSRDKPGLNINLSAALTDYVTCDDAVAEGGPSSSVTRTNVMLRQMASNCLYLSSLIRL 184
           +LK+ DKP LNINLSAALT YVTCDDAVAE G +S VTRTN+ LRQMA+NCLYLSSLI +
Sbjct: 124 TLKTGDKPTLNINLSAALTGYVTCDDAVAERGGTSPVTRTNLTLRQMATNCLYLSSLIGV 178

[Close]

BLAST of Tan0021958 vs. NCBI nr
Match: XP_022131971.1 (pectinesterase inhibitor 12-like [Momordica charantia])

HSP 1 Score: 220.3 bits (560), Expect = 1.4e-53
Identity = 118/180 (65.56%), Postives = 136/180 (75.56%), Query Frame = 0


Query: 9   SAYCCLLLVA--MAGMWQSG--EGQEQERERELLSMNDLNVNLGILDICGNADYPALCRS 68
           ++  CL  VA  MAGMW  G  +GQ QERERELL ++ +N+NLG  DIC  ADYPA+CRS
Sbjct: 2   ASQACLFFVALLMAGMWYGGHAQGQSQERERELLGLS-INLNLG-FDICRKADYPAMCRS 61

Query: 69  VVKGERNPSVAIESAIRQLMFQTDMAKGVAQRRKKSEGAMDVCFENYDDAFSNLETCLQS 128
           VVKG  NP VA E+A+RQLMFQT  AK VAQR K    AM+VCFE YDDA+SNLET L +
Sbjct: 62  VVKGLNNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSN 121

Query: 129 LKSRDKPGLNINLSAALTDYVTCDDAVAEGGPSSSVTRTNVMLRQMASNCLYLSSLIRLR 185
           LKS DK  LN++LSA LTDYVTCDDA+ E G +S VTRTN +L QMASNCLY SSLI LR
Sbjct: 122 LKSHDKSSLNVDLSAVLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLIHLR 179

[Close]

BLAST of Tan0021958 vs. NCBI nr
Match: XP_023002765.1 (pectinesterase 3-like [Cucurbita maxima])

HSP 1 Score: 219.2 bits (557), Expect = 3.0e-53
Identity = 118/172 (68.60%), Postives = 136/172 (79.07%), Query Frame = 0


Query: 13  CLLLVAMAGMWQSGEGQEQERERELLSMNDLNVNLGILDICGNADYPALCRSVVKGERNP 72
           CLLLVA AGMWQ G+GQ    ERELLS+N       + D+CG A+YP LCRS+VKG  NP
Sbjct: 11  CLLLVATAGMWQGGQGQ----ERELLSLN-------MGDLCGKANYPVLCRSMVKGLSNP 70

Query: 73  SVAIESAIRQLMFQTDMAKGVAQRRKKSEGAMDVCFENYDDAFSNLETCLQSLKSRDKPG 132
           SVA+ESAIRQLM QT MA+  A+R +KS G M+VC E Y DAFSNL+TCL +LK+ DKP 
Sbjct: 71  SVALESAIRQLMLQTKMARTTARRLRKSPG-MEVCSETYQDAFSNLKTCLSTLKTGDKPT 130

Query: 133 LNINLSAALTDYVTCDDAVAEGGPSSSVTRTNVMLRQMASNCLYLSSLIRLR 185
           LNINLSAALTDYVTCDDAVAE G +S VTRTN+ LRQMA+NCLYLSSLI +R
Sbjct: 131 LNINLSAALTDYVTCDDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLIGVR 170

[Close]

BLAST of Tan0021958 vs. NCBI nr
Match: KAG6585173.1 (hypothetical protein SDJN03_17906, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 211.8 bits (538), Expect = 4.9e-51
Identity = 115/166 (69.28%), Postives = 132/166 (79.52%), Query Frame = 0


Query: 19  MAGMWQSGEGQEQERERELLSMNDLNVNLGILDICGNADYPALCRSVVKGERNPSVAIES 78
           MAGMW+ G+GQEQ  ERELLS+N L  N+G  D+CG ADYP LCRSVVKG   PSVA+ES
Sbjct: 1   MAGMWEGGQGQEQ--ERELLSLN-LYPNMG--DLCGKADYPVLCRSVVKGLSRPSVALES 60

Query: 79  AIRQLMFQTDMAKGVAQRRKKSEGAMDVCFENYDDAFSNLETCLQSLKSRDKPGLNINLS 138
           AIRQLM QT MA+  A+R +KS G M+VC E Y DAF NL+TCL +LK+ DKP LNINLS
Sbjct: 61  AIRQLMLQTKMARTTAKRLRKSPG-MEVCSETYQDAFRNLKTCLSTLKTGDKPTLNINLS 120

Query: 139 AALTDYVTCDDAVAEGGPSSSVTRTNVMLRQMASNCLYLSSLIRLR 185
           AALT YVTCDDAV E G +S VTRTN+ LRQMA+NCLYLSSLI +R
Sbjct: 121 AALTGYVTCDDAVVERGGTSPVTRTNLTLRQMATNCLYLSSLIGVR 160

[Close]

BLAST of Tan0021958 vs. NCBI nr
Match: XP_022951850.1 (pectinesterase inhibitor 12-like [Cucurbita moschata])

HSP 1 Score: 203.8 bits (517), Expect = 1.3e-48
Identity = 110/163 (67.48%), Postives = 128/163 (78.53%), Query Frame = 0


Query: 19  MAGMWQSGEGQEQERERELLSMNDLNVNLGILDICGNADYPALCRSVVKGERNPSVAIES 78
           MAGMW+ G+GQ     RELLS+N L+ N+G  D+CG ADYP LCRSVVKG   PSVA+ES
Sbjct: 1   MAGMWEGGQGQ----GRELLSLN-LDPNMG--DLCGKADYPVLCRSVVKGVSKPSVALES 60

Query: 79  AIRQLMFQTDMAKGVAQRRKKSEGAMDVCFENYDDAFSNLETCLQSLKSRDKPGLNINLS 138
           AIRQLM QT MA+  A+R +KS G ++VC E Y DAF NL+TCL +LK+ DKP LNINLS
Sbjct: 61  AIRQLMLQTKMARTTAKRLRKSPG-VEVCSETYQDAFRNLKTCLSTLKTGDKPTLNINLS 120

Query: 139 AALTDYVTCDDAVAEGGPSSSVTRTNVMLRQMASNCLYLSSLI 182
           AALT YVTCDDAV E G +S VTRTN+ LRQMA+NCLYLSSLI
Sbjct: 121 AALTGYVTCDDAVVERGGTSPVTRTNLTLRQMATNCLYLSSLI 155

[Close]

BLAST of Tan0021958 vs. ExPASy TrEMBL
Match: A0A6J1BQY5 (pectinesterase inhibitor 12-like OS=Momordica charantia OX=3673 GN=LOC111004955 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 6.6e-54
Identity = 118/180 (65.56%), Postives = 136/180 (75.56%), Query Frame = 0


Query: 9   SAYCCLLLVA--MAGMWQSG--EGQEQERERELLSMNDLNVNLGILDICGNADYPALCRS 68
           ++  CL  VA  MAGMW  G  +GQ QERERELL ++ +N+NLG  DIC  ADYPA+CRS
Sbjct: 2   ASQACLFFVALLMAGMWYGGHAQGQSQERERELLGLS-INLNLG-FDICRKADYPAMCRS 61

Query: 69  VVKGERNPSVAIESAIRQLMFQTDMAKGVAQRRKKSEGAMDVCFENYDDAFSNLETCLQS 128
           VVKG  NP VA E+A+RQLMFQT  AK VAQR K    AM+VCFE YDDA+SNLET L +
Sbjct: 62  VVKGLNNPRVAFEAAVRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSN 121

Query: 129 LKSRDKPGLNINLSAALTDYVTCDDAVAEGGPSSSVTRTNVMLRQMASNCLYLSSLIRLR 185
           LKS DK  LN++LSA LTDYVTCDDA+ E G +S VTRTN +L QMASNCLY SSLI LR
Sbjct: 122 LKSHDKSSLNVDLSAVLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLIHLR 179

[Close]

BLAST of Tan0021958 vs. ExPASy TrEMBL
Match: A0A6J1KPW1 (pectinesterase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111496527 PE=4 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 1.5e-53
Identity = 118/172 (68.60%), Postives = 136/172 (79.07%), Query Frame = 0


Query: 13  CLLLVAMAGMWQSGEGQEQERERELLSMNDLNVNLGILDICGNADYPALCRSVVKGERNP 72
           CLLLVA AGMWQ G+GQ    ERELLS+N       + D+CG A+YP LCRS+VKG  NP
Sbjct: 11  CLLLVATAGMWQGGQGQ----ERELLSLN-------MGDLCGKANYPVLCRSMVKGLSNP 70

Query: 73  SVAIESAIRQLMFQTDMAKGVAQRRKKSEGAMDVCFENYDDAFSNLETCLQSLKSRDKPG 132
           SVA+ESAIRQLM QT MA+  A+R +KS G M+VC E Y DAFSNL+TCL +LK+ DKP 
Sbjct: 71  SVALESAIRQLMLQTKMARTTARRLRKSPG-MEVCSETYQDAFSNLKTCLSTLKTGDKPT 130

Query: 133 LNINLSAALTDYVTCDDAVAEGGPSSSVTRTNVMLRQMASNCLYLSSLIRLR 185
           LNINLSAALTDYVTCDDAVAE G +S VTRTN+ LRQMA+NCLYLSSLI +R
Sbjct: 131 LNINLSAALTDYVTCDDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLIGVR 170

[Close]

BLAST of Tan0021958 vs. ExPASy TrEMBL
Match: A0A6J1GK21 (pectinesterase inhibitor 12-like OS=Cucurbita moschata OX=3662 GN=LOC111454582 PE=4 SV=1)

HSP 1 Score: 203.8 bits (517), Expect = 6.4e-49
Identity = 110/163 (67.48%), Postives = 128/163 (78.53%), Query Frame = 0


Query: 19  MAGMWQSGEGQEQERERELLSMNDLNVNLGILDICGNADYPALCRSVVKGERNPSVAIES 78
           MAGMW+ G+GQ     RELLS+N L+ N+G  D+CG ADYP LCRSVVKG   PSVA+ES
Sbjct: 1   MAGMWEGGQGQ----GRELLSLN-LDPNMG--DLCGKADYPVLCRSVVKGVSKPSVALES 60

Query: 79  AIRQLMFQTDMAKGVAQRRKKSEGAMDVCFENYDDAFSNLETCLQSLKSRDKPGLNINLS 138
           AIRQLM QT MA+  A+R +KS G ++VC E Y DAF NL+TCL +LK+ DKP LNINLS
Sbjct: 61  AIRQLMLQTKMARTTAKRLRKSPG-VEVCSETYQDAFRNLKTCLSTLKTGDKPTLNINLS 120

Query: 139 AALTDYVTCDDAVAEGGPSSSVTRTNVMLRQMASNCLYLSSLI 182
           AALT YVTCDDAV E G +S VTRTN+ LRQMA+NCLYLSSLI
Sbjct: 121 AALTGYVTCDDAVVERGGTSPVTRTNLTLRQMATNCLYLSSLI 155

[Close]

BLAST of Tan0021958 vs. ExPASy TrEMBL
Match: A0A0A0LNC2 (Invertase/pectin methylesterase inhibitor family protein OS=Cucumis sativus OX=3659 GN=Csa_2G348980 PE=4 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 6.9e-43
Identity = 105/178 (58.99%), Postives = 125/178 (70.22%), Query Frame = 0


Query: 6   SHYSAYCCLLLVAMAGMWQSGEGQEQERERELLSMNDLNVNLGILDICGNADYPALCRSV 65
           S  S +  L+L+ +A M  S  G E        +   LN+NL  LD+C  ADYPALCRSV
Sbjct: 11  SSCSNFYLLMLLVVANMLWSSWGSE--------AALTLNLNLS-LDLCKKADYPALCRSV 70

Query: 66  VKGERNPSVAIESAIRQLMFQTDMAKGVAQRRKKSEGAMDVCFENYDDAFSNLETCLQSL 125
           VKG  +PS A+E++I+QLM +T  A  VA+R+K S  AMDVC E YDDA+SNLETCL SL
Sbjct: 71  VKGLVDPSTAMETSIKQLMVETKQAMSVAKRQKSS--AMDVCIEVYDDAYSNLETCLSSL 130

Query: 126 KSRDKPGLNINLSAALTDYVTCDDAVAEGGPSSSVTRTNVMLRQMASNCLYLSSLIRL 184
           KS DK  LNINLSAALTDYVTC DA+AE G SS +TR N +L QM +NCLYLS LIRL
Sbjct: 131 KSHDKGTLNINLSAALTDYVTCQDAIAEKGLSSPITRNNNLLSQMTTNCLYLSGLIRL 177

[Close]

BLAST of Tan0021958 vs. ExPASy TrEMBL
Match: A0A5A7V5S8 (Putative pectinesterase/pectinesterase inhibitor 40 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G002070 PE=4 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 5.8e-42
Identity = 104/179 (58.10%), Postives = 126/179 (70.39%), Query Frame = 0


Query: 9   SAYCC----LLLVAMAGMWQSGEGQEQERERELLSMNDLNVNLGILDICGNADYPALCRS 68
           S  CC    L L+ +A +W S  G E        +   LN+NL  LD+C  ADYPALCRS
Sbjct: 8   STVCCNNFYLPLLVVAMLW-SSPGSE--------AALTLNLNLS-LDLCKKADYPALCRS 67

Query: 69  VVKGERNPSVAIESAIRQLMFQTDMAKGVAQRRKKSEGAMDVCFENYDDAFSNLETCLQS 128
           VVKG  +PS A+E++I++LM +T  A  VA+R+K S  AMDVC E YDDA+SNLET L S
Sbjct: 68  VVKGLTDPSTAMETSIKKLMVETKQAMSVAKRQKSS--AMDVCIEVYDDAYSNLETSLSS 127

Query: 129 LKSRDKPGLNINLSAALTDYVTCDDAVAEGGPSSSVTRTNVMLRQMASNCLYLSSLIRL 184
           LKS DK  LNINLSA+LTDYVTCDDA+AE G +S VTR N +LR+M +NCLYLS LIRL
Sbjct: 128 LKSHDKGTLNINLSASLTDYVTCDDAIAERGSTSPVTRNNKLLREMTTNCLYLSGLIRL 174

[Close]

BLAST of Tan0021958 vs. TAIR 10
Match: AT1G70540.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 120.6 bits (301), Expect = 1.4e-27
Identity = 60/132 (45.45%), Postives = 87/132 (65.91%), Query Frame = 0


Query: 51  DICGNADYPALCRSVVKGERNPSVAIESAIRQLMFQTDMAKGVAQRRKKSEGAMDVCFEN 110
           D C  +D+  LCRSVVKG++N + A E +IR+LM +T  AK  A+  +KS G +  C+ N
Sbjct: 35  DPCSVSDFKVLCRSVVKGQKNVNAATEVSIRELMKRTIKAKEAAKISRKSGGGLKTCYSN 94

Query: 111 YDDAFSNLETCLQSLKSRDKPGLNINLSAALTDYVTCDDAVAEGGPSSSVTRTNVMLRQM 170
           YD A  NL+  L+++K  D   LNINLSA+LTD+ TC+DA+  G  S+   ++   L +M
Sbjct: 95  YDSALENLQKALKNIKQNDGFSLNINLSASLTDFDTCNDAMGGGTASNVFAKSTSTLHEM 154

Query: 171 ASNCLYLSSLIR 183
           A NCL LS+L++
Sbjct: 155 ADNCLALSTLVK 166

[Close]

BLAST of Tan0021958 vs. TAIR 10
Match: AT1G23350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 119.8 bits (299), Expect = 2.3e-27
Identity = 58/138 (42.03%), Postives = 91/138 (65.94%), Query Frame = 0


Query: 45  VNLGILDICGNADYPALCRSVVKGERNPSVAIESAIRQLMFQTDMAKGVAQRRKKSEGAM 104
           +++ + D C  +D+PALCRS +KG+ N + A + AI++LM +T  AK +A++  K +G +
Sbjct: 21  LSISLADPCTVSDFPALCRSTIKGQTNVNAATDVAIKELMKRTRQAKKIAEKELKRDGGV 80

Query: 105 DVCFENYDDAFSNLETCLQSLKSRDKPGLNINLSAALTDYVTCDDAVAEGGPSSSVTRTN 164
             C  N++ AF NLE  L ++K  D   LNINLSAALTDY TC D++ E    + + ++ 
Sbjct: 81  ATCLSNFNSAFDNLEKALTNIKENDGFSLNINLSAALTDYDTCSDSMKETKEVNVIYKSA 140

Query: 165 VMLRQMASNCLYLSSLIR 183
            +L +MA NCL LS+L++
Sbjct: 141 GVLYRMADNCLALSTLVK 158

[Close]

BLAST of Tan0021958 vs. TAIR 10
Match: AT1G10770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 92.4 bits (228), Expect = 4.0e-19
Identity = 49/134 (36.57%), Postives = 76/134 (56.72%), Query Frame = 0


Query: 49  ILDICGNADYPALCRSVVKGERNPSVAIESAIRQLMFQTDMAKGVAQRRKKSEGAMDVCF 108
           +++IC +  YP+LCR +VK   +P  A    I+ L  +T +A     R K    A+  C+
Sbjct: 34  MINICSHTAYPSLCRPLVKRVTSPRKATHRTIQALEAKTKLALAETARFKNGNQAVSTCY 93

Query: 109 ENYDDAFSNLETCLQSLKSRDKPGLNINLSAALTDYVTCDDAVAEGGPSSSVTRTNVMLR 168
           E   DA  NL +  +S++ RD P +N  L+AA++DY  C D   E    +++    V LR
Sbjct: 94  ETLGDAVYNLASARKSIRKRDVPAMNTYLTAAVSDYGACVDGFIETQQVNAIQNAVVDLR 153

Query: 169 QMASNCLYLSSLIR 183
           +++SNCL LS+LIR
Sbjct: 154 KISSNCLALSTLIR 167

[Close]

BLAST of Tan0021958 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 81.3 bits (199), Expect = 9.2e-16
Identity = 52/140 (37.14%), Postives = 72/140 (51.43%), Query Frame = 0


Query: 49  ILDICGNADYPALCRSVVKG------ERNPSVAIESAIRQLMFQTDMAKGVAQRRKKSEG 108
           I  ICG  D P LC S V        + N S  +  AI+  +  T  A  + ++   S+ 
Sbjct: 130 IKTICGKTDNPPLCESSVSPLLTPQLKPNTSSVLILAIQASITATKAAMAIVEKVDASD- 189

Query: 109 AMDVCFENYDDAFSNLETCLQSLKSRDKPGLNINLSAALTDYVTCDDAVAEGGPSSSVTR 168
               C E YDDA  NLE  + ++KS D   +N NLSAA+TDY TC+D   E G  + +  
Sbjct: 190 ----CQELYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGFEESGEPNPLAY 249

Query: 169 TNVMLRQMASNCLYLSSLIR 183
               L +M SNCL +S+LI+
Sbjct: 250 VADKLTKMVSNCLAISTLIK 264

[Close]

BLAST of Tan0021958 vs. TAIR 10
Match: AT1G60760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 72.0 bits (175), Expect = 5.6e-13
Identity = 44/131 (33.59%), Postives = 68/131 (51.91%), Query Frame = 0


Query: 52  ICGNADYPALCRSVVKGERNPSVAIESAIRQLMFQTDMAKGVAQRRKKSEGAMDVCFENY 111
           +C    YP LCR +VKG  NP  A  S IR L  +T +A   + R K     + +C+   
Sbjct: 34  LCAQTAYPTLCRPLVKGP-NPRRATHSTIRALEAKTKLAITDSARFKSGNPTVAICYATL 93

Query: 112 DDAFSNLETCLQSLKSRDKPGLNINLSAALTDYVTCDDAVAEGGPSSSVTRTNVMLRQMA 171
            DA  NL     S++ R+   L + L+AA++DY  C +   +    +++      LR+M 
Sbjct: 94  VDASFNLRRARTSIRKRNVMMLKMFLTAAVSDYGVCVNGFIDSHQVNTLQNDVDELRKMG 153

Query: 172 SNCLYLSSLIR 183
           +NCL L++LIR
Sbjct: 154 TNCLLLATLIR 163

The following BLAST results are available for this feature:

BLAST of Tan0021958 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FJR5	1.4e-05	37.35	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]
Q9LUV1	9.3e-05	26.24	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]
A9YUH4	7.9e-04	35.53	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]

BLAST of Tan0021958 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023536926.1	5.9e-57	69.06	uncharacterized protein LOC111798158 [Cucurbita pepo subsp. pepo]	[more]
XP_022131971.1	1.4e-53	65.56	pectinesterase inhibitor 12-like [Momordica charantia]	[more]
XP_023002765.1	3.0e-53	68.60	pectinesterase 3-like [Cucurbita maxima]	[more]
KAG6585173.1	4.9e-51	69.28	hypothetical protein SDJN03_17906, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022951850.1	1.3e-48	67.48	pectinesterase inhibitor 12-like [Cucurbita moschata]	[more]

BLAST of Tan0021958 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BQY5	6.6e-54	65.56	pectinesterase inhibitor 12-like OS=Momordica charantia OX=3673 GN=LOC111004955 ...	[more]
A0A6J1KPW1	1.5e-53	68.60	pectinesterase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111496527 PE=4 SV=1	[more]
A0A6J1GK21	6.4e-49	67.48	pectinesterase inhibitor 12-like OS=Cucurbita moschata OX=3662 GN=LOC111454582 P...	[more]
A0A0A0LNC2	6.9e-43	58.99	Invertase/pectin methylesterase inhibitor family protein OS=Cucumis sativus OX=3...	[more]
A0A5A7V5S8	5.8e-42	58.10	Putative pectinesterase/pectinesterase inhibitor 40 OS=Cucumis melo var. makuwa ...	[more]

BLAST of Tan0021958 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G70540.1	1.4e-27	45.45	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G23350.1	2.3e-27	42.03	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G10770.1	4.0e-19	36.57	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G36659.1	9.2e-16	37.14	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G60760.1	5.6e-13	33.59	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 44..177 e-value: 4.4E-25 score: 99.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 51..176 e-value: 6.0E-18 score: 65.6
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 90..179 e-value: 1.2E-15 score: 55.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 48..182 e-value: 5.2E-22 score: 80.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 51..181
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 46..181
None	No IPR available	PANTHER	PTHR31707:SF270	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 46..181
None	No IPR available	CDD	cd15800	PMEI-like_2	coord: 51..181 e-value: 4.49121E-43 score: 139.032

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0021958.1	Tan0021958.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity