Tan0021886 (gene) Snake gourd v1

Overview
NameTan0021886
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionUPF0496 protein At3g49070
LocationLG07: 7306101 .. 7310197 (+)
RNA-Seq ExpressionTan0021886
SyntenyTan0021886
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
CAACATTTGAGAATTTAATTTGATTTCGTTCAGATTATTCATATTTTCACAGACAGACATGAAGAAATCAAAGATTGTTTCACGTCTCAGAATATTCTTTTCATGCTCTGGTAATAATTCTAACCAATTCTCTTTTATATATTCATCAATATTTTTTAAAAAAAAAAAATGTTAAAGGCTAAATATATGTTTTGGTGCTGATGTTTCAAAAGTTTTTGTTTTTCATCGATCTTTAATGTATTGAAATTTCTTCAATTAACCTCTTATCGTCACACCATTACCATACATTAGAACTAATTCACATATTGATGATGTAATTTCAAAATACATGTTTTTACCGTGAGTTACAGAAAGTATTCGATGAGTTAATTTCATAAAATAGACAATTACTCACTTGCAAGTAGGAGTGTACAACAAACCCAGTAACCTGAACAATCGGGACTACTCAACCCAATCCGATTGGATTGGGTTAGATCTTTTTCGGTTTGGGTTGAGTTAGAAATTTGAAGAACCGAAAAGTTCGGGTTAGTTCTCGAGCATTTTAGGCTCGAATTGACCCAACCCAAAGATTACATTTTTTTTTTAATTTTATTTTAGTTATATATATACTTAAAATGAATTATTTGTTAATTTTTTATGTTTTTTTTAGACTTCTAATAAATGTTAAGATGGTATAAACTTTGAAGTTTTATATTTGAATTTTATGTGATTTTAGTTATTAATTCAAGAAAAAATATTTTAAATAATTAAAAAATTATAAACAAATAAAAAAAATAACCCATCGATCCAACCCGAAAATATTGGGTTGGGTTGGGTTACTCTCATGAATAGGGTTGGTTAGGTTGAAGGAATGACTAACCCGAATTTTCGGATTTGTCCAAAAATCTCCACCAACCCAACCTAGTTTATGTACACTCCTACTTGTAAGGTTAAAAAATTGCTCATTTTGTATGAATTACTACATTATAACTTACTCGACTTTATATTATTCATTGTTATAAAGAACTTATTATTTTCAAATATTTATGTGTTTTCATTCTTTTAAGTAATTCTTAGAATTAAATCCAAAAATTAATACACAAAAATTGAAATTGAAACTTCTAAAAGAGACCAAATTAAAAAAACACACACAAATAGAGAGACTAAAACATATACTTTGACTTGCTTAATTTATGAGTTTAATCTCTATGTTTCAGTCAAAGCTTTAATTCGGTCTCTACGATTTTAAAAGTTTTAGTTGGTCCCTTATAGTTTGAATTTTGTTTTAATGTATATTCTTTATGCTTTTAAATTTTTCAAAATTTGTTTTTATGGTTTTGTGAAATCTAAATCATCCCTATTGTCAAGATGATTTTTTGAAAGATATGTCTATGGTCAATTATATTATTGAACATATGACGTTTTTTAATTATGAAACAATGAGCACGGTATTGAGCAATTTGGCTAAATTGCATTAGTTTACTTTATTAGAAATTTAGGCTAAATAAAACAAATATAAAATATATATGAGATTCACTTAAACTAAAGAATTTTTTTTAGACGACTAGAGATTAAATTAAAAAATTTAAAACGTAAAGTTTAAATTAAAAATTTTGAAATCATAGACACCAATTTAAAATTAAGCCCAAGACCAAATTTGTAATTTAAAATTATTTTTTCCTAGATGTAATTTAGTCAAATTCAATCTTTCAAAATTTAACATAAAGACTTTTTTTTCCCTTCTTCTTTAGGGTTAGGTTTCAAGAAAGAGTTTTCTTTTTAATTCAAAAAATTAATAGAGATTTGTTAATCGTTAGGGTTAACCTTTCAAGAAGAATATTATTTGTATTACTTTGTGGTTGGCTGAAATTAAAATCTGAAGATAAGATGAGTTTTTCCTCCTTTTTTTTCCCTCCTTTTCCATCTCAGCTCCAACCACTTGGTCTGAAGGCTAATTTTTGAAATTTTATTTTTTATACAAAACTTATCAAAACGTGTATTAAAGGATGTTTGAGAAAAAAAAATTATAGTAAAATGCAAATAGTTTTCCCAAAGGTTTTTAGTGTTGGCAATAACCACTCTCTAAAGTTAGTAAACTCTATCATTATTACCAAGTAACTATAGTTTAGCGATAAATTGACATGTAAATTTTCTTTCTCTTGAAATCGAAGGTTCCAATTTCCATACTCTCACTTGTTGCACTAAAAAAACTTAATAAACTAACATAAAACACTAATCAGTATCAAGAATGGATTAGAATTAATTGTTAGAGTTGGTTCACTAGCTATCCATCATTAAAGATGTAATATCACATATTAGGGGTGTACAAATAACCCAAGCAATCCGGACTGCCCAACCTAAACTGTAAGAGTTAGGTTGAGTTAGGTTTTTTTTTTCGGTTTGGGTTGGATTAGAAGTTTGGAGAAACCGAACAGTTCGAATCGGTTCTCGAGTTGAGTTTTTTAATCTCAGGTTGATCCAACCACACCCAACCCAACTTAACGTTTATATTTTTTTAATTTTATTTTCAATTACATATATACTTATAAATGAATTATTGGTCAATTTTTGACTTCTAATAAATATTAAGATGTTACGAAATTTGAAGTTTGATATTTGAATTTTATGTGATTTTAGTTATTAATTAAAGAAAATTATTTTAAATAATTAAAAAATTATAAAAAAAAAATTAACCCAACGACCCAAACGAACCCAACCAAAAAATATTGAGTTGGGTTAGGTTACTCTCATGAATAGAGTTTGTTGGGTTGAAGGAATGACCAACTCGACTTATGTACACGTACACCCTAGACACACACTTTATTTTATACCACGTTTAAAATATCATATAGATAAAAATATGGAAATATCACTTTATATAAAAAAAAAGTTTATAAAACTATCGATACAATGTTATGGATAATTCTAAAAAATATTAGTAAAAAATTAATAAAAAAATATATAAATTAACCGATAAACATTTCACTCTTTTTAAATAAATTTAAATTTCTTATTATTTATATTATTATTTTTGTAGATATTTGAATTATTTTTCATTAATTTTTCTAGGACATGATCATTAATTATATGTTTAATTAATGTGGATGTTATATGATATAATAACTTTTTTCGTGTTGAATTGAATAGATCGTGGGCCGCCACACATAAGAAACCATCCAATAAGCGTAGACGTACGGGAGGAGTACGCCAACGCCTTCCGCACCGAATCATACCTCGAGTTCTGGACACGTGTCGTGGCGTTAAACGATGGAGCAATGGAGTCCACAACCGCAACCCGGCTCTCCTCTTATAGGCTATTCGCTGAACATCTACTGGACCCAACTGATCCCACAGTCAAAACAATCCTAAGTTCAGCGGCCCATCTCCGGCCCAATTCCATCTCCCTCCTCTCCGATTACTTCTCTCACACCGCCAACGCCTCCCTCTTCTGCGGCCGCTTACTCAAAACCCTCGACCATTTCCGCCGCAAACTCCGTCCCTTCAAGCAATTTCACCACAACCCATTTGTACAATGTGCAGAAGGACTCCAAATCATCCAAACCGGATGCTCCAAATTGCTGAAGCGGCTCGAGTTCAACCGCGACAGGGCTCGAGCCGAGCGCAGGAGAGTCAGATATTTCCAACACGGTTTGGGTGGGTTGTTGGTGGCCGTGACGGCGTCGCTTACGGTAATAGTGGTGACTCATGGAATGGCGCTGTTGGTGGCTGCGCCGGGGCTACTTGTGGGTGCTGTAAAGTTAGGCGCCAAGTCGGGGAAGGTGGGTCGAGTGCTGGATGTGGCTGCGAAAGGGACTTACACTTTGAATAGGGATTTCGACACGGTGGCTCGGGTCGTGGCTCGGGTGAGTCAGGAGGTGGAACGAATGAGGGTATTGATGAGGTTTTGGGTTGAGAGAGGGGAGGATTGTGGCCGTGGACGTGAACCCATTGGTGAAGTGGCGCGCCAGTTGAAACAGAGTTGTGTGAACTTTAGTGAACAGTTGGATGAACTTGAGGAGCATTTGTATTTGTGTTTTATGACCATAAATCGAGCTAGAAATCTTGTAGTCAAAGAGATTCTGGATTCGGGTCAACCTGTAAAGACTCCATATATATGA

mRNA sequence

CAACATTTGAGAATTTAATTTGATTTCGTTCAGATTATTCATATTTTCACAGACAGACATGAAGAAATCAAAGATTGTTTCACGTCTCAGAATATTCTTTTCATGCTCTGATCGTGGGCCGCCACACATAAGAAACCATCCAATAAGCGTAGACGTACGGGAGGAGTACGCCAACGCCTTCCGCACCGAATCATACCTCGAGTTCTGGACACGTGTCGTGGCGTTAAACGATGGAGCAATGGAGTCCACAACCGCAACCCGGCTCTCCTCTTATAGGCTATTCGCTGAACATCTACTGGACCCAACTGATCCCACAGTCAAAACAATCCTAAGTTCAGCGGCCCATCTCCGGCCCAATTCCATCTCCCTCCTCTCCGATTACTTCTCTCACACCGCCAACGCCTCCCTCTTCTGCGGCCGCTTACTCAAAACCCTCGACCATTTCCGCCGCAAACTCCGTCCCTTCAAGCAATTTCACCACAACCCATTTGTACAATGTGCAGAAGGACTCCAAATCATCCAAACCGGATGCTCCAAATTGCTGAAGCGGCTCGAGTTCAACCGCGACAGGGCTCGAGCCGAGCGCAGGAGAGTCAGATATTTCCAACACGGTTTGGGTGGGTTGTTGGTGGCCGTGACGGCGTCGCTTACGGTAATAGTGGTGACTCATGGAATGGCGCTGTTGGTGGCTGCGCCGGGGCTACTTGTGGGTGCTGTAAAGTTAGGCGCCAAGTCGGGGAAGGTGGGTCGAGTGCTGGATGTGGCTGCGAAAGGGACTTACACTTTGAATAGGGATTTCGACACGGTGGCTCGGGTCGTGGCTCGGGTGAGTCAGGAGGTGGAACGAATGAGGGTATTGATGAGGTTTTGGGTTGAGAGAGGGGAGGATTGTGGCCGTGGACGTGAACCCATTGGTGAAGTGGCGCGCCAGTTGAAACAGAGTTGTGTGAACTTTAGTGAACAGTTGGATGAACTTGAGGAGCATTTGTATTTGTGTTTTATGACCATAAATCGAGCTAGAAATCTTGTAGTCAAAGAGATTCTGGATTCGGGTCAACCTGTAAAGACTCCATATATATGA

Coding sequence (CDS)

ATGAAGAAATCAAAGATTGTTTCACGTCTCAGAATATTCTTTTCATGCTCTGATCGTGGGCCGCCACACATAAGAAACCATCCAATAAGCGTAGACGTACGGGAGGAGTACGCCAACGCCTTCCGCACCGAATCATACCTCGAGTTCTGGACACGTGTCGTGGCGTTAAACGATGGAGCAATGGAGTCCACAACCGCAACCCGGCTCTCCTCTTATAGGCTATTCGCTGAACATCTACTGGACCCAACTGATCCCACAGTCAAAACAATCCTAAGTTCAGCGGCCCATCTCCGGCCCAATTCCATCTCCCTCCTCTCCGATTACTTCTCTCACACCGCCAACGCCTCCCTCTTCTGCGGCCGCTTACTCAAAACCCTCGACCATTTCCGCCGCAAACTCCGTCCCTTCAAGCAATTTCACCACAACCCATTTGTACAATGTGCAGAAGGACTCCAAATCATCCAAACCGGATGCTCCAAATTGCTGAAGCGGCTCGAGTTCAACCGCGACAGGGCTCGAGCCGAGCGCAGGAGAGTCAGATATTTCCAACACGGTTTGGGTGGGTTGTTGGTGGCCGTGACGGCGTCGCTTACGGTAATAGTGGTGACTCATGGAATGGCGCTGTTGGTGGCTGCGCCGGGGCTACTTGTGGGTGCTGTAAAGTTAGGCGCCAAGTCGGGGAAGGTGGGTCGAGTGCTGGATGTGGCTGCGAAAGGGACTTACACTTTGAATAGGGATTTCGACACGGTGGCTCGGGTCGTGGCTCGGGTGAGTCAGGAGGTGGAACGAATGAGGGTATTGATGAGGTTTTGGGTTGAGAGAGGGGAGGATTGTGGCCGTGGACGTGAACCCATTGGTGAAGTGGCGCGCCAGTTGAAACAGAGTTGTGTGAACTTTAGTGAACAGTTGGATGAACTTGAGGAGCATTTGTATTTGTGTTTTATGACCATAAATCGAGCTAGAAATCTTGTAGTCAAAGAGATTCTGGATTCGGGTCAACCTGTAAAGACTCCATATATATGA

Protein sequence

MKKSKIVSRLRIFFSCSDRGPPHIRNHPISVDVREEYANAFRTESYLEFWTRVVALNDGAMESTTATRLSSYRLFAEHLLDPTDPTVKTILSSAAHLRPNSISLLSDYFSHTANASLFCGRLLKTLDHFRRKLRPFKQFHHNPFVQCAEGLQIIQTGCSKLLKRLEFNRDRARAERRRVRYFQHGLGGLLVAVTASLTVIVVTHGMALLVAAPGLLVGAVKLGAKSGKVGRVLDVAAKGTYTLNRDFDTVARVVARVSQEVERMRVLMRFWVERGEDCGRGREPIGEVARQLKQSCVNFSEQLDELEEHLYLCFMTINRARNLVVKEILDSGQPVKTPYI
Homology
BLAST of Tan0021886 vs. ExPASy Swiss-Prot
Match: Q9SMU4 (UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1)

HSP 1 Score: 267.3 bits (682), Expect = 2.4e-70
Identity = 169/368 (45.92%), Postives = 216/368 (58.70%), Query Frame = 0

Query: 5   KIVSRLRIFFSCSDRGPPHIRNHP---ISVDVREEYANAFRTESYLEFWTRVVAL----- 64
           K V++  I    S  G     ++P     VDVREEYANAFRTESY  FWTRVV L     
Sbjct: 40  KFVTKKIIILKLSSIGTASGSSNPKDGADVDVREEYANAFRTESYNHFWTRVVQLSRKKS 99

Query: 65  -------NDGAMESTTATRLSSYRLFAEHLLDPTDPTVKTILSSAAHLRPNSISLLSDYF 124
                  +     S+T+ RL SYRLFA +LLDP   T+  IL   + +  ++ +LLSDYF
Sbjct: 100 AVSSSSSSPPIESSSTSARLMSYRLFAHNLLDPDLNTITRIL-DVSRVGRHTRTLLSDYF 159

Query: 125 SHTANASLFCGRLLKTLDHFRRKLRPFK-QFH---HN----------------PFVQCAE 184
             TANA L C +LLK + H R K    K +FH   HN                PF+    
Sbjct: 160 LETANAFLLCTQLLKNIHHLRSKYESLKPKFHSENHNSLALIDQFTEISKWFDPFISSGS 219

Query: 185 GLQIIQTGCSKLLKRLEFNRDRARAERRRVRYFQHGLGGLLVAVTASLTVIVVTHGMALL 244
            +Q+I++GC  LLKRLE  RD+ RA+ + +    H  G L++A+T +L V + +H  AL 
Sbjct: 220 RIQLIRSGCLYLLKRLESRRDKTRAKLKLINGLTHSSGLLVLALTTTLIVTIASHAFALF 279

Query: 245 VAAPGLLVGAVK---LGAKSGKVGRVLDVAAKGTYTLNRDFDTVARVVARVSQEVERMRV 304
           +AAP LL    K   L  K  K    LDVAAKGTY L+RD DT++R+V R++ EV  +R 
Sbjct: 280 LAAPTLLASQFKPAGLRNKLTKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRA 339

Query: 305 LMRFWVERGEDCGRGREPIGEVARQLKQSCVNFSEQLDELEEHLYLCFMTINRARNLVVK 335
           +  FWV RG    RG E   EVAR+LK+   +FSE+LDELEEH+YLCFMTINRARNL+VK
Sbjct: 340 MAEFWVGRGSGRVRGSE---EVARELKRCEESFSEELDELEEHIYLCFMTINRARNLLVK 399

BLAST of Tan0021886 vs. ExPASy Swiss-Prot
Match: A2XCJ1 (UPF0496 protein 3 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_009784 PE=3 SV=2)

HSP 1 Score: 186.4 bits (472), Expect = 5.3e-46
Identity = 133/340 (39.12%), Postives = 177/340 (52.06%), Query Frame = 0

Query: 30  SVDVREEYANAFRTESYLEFWTRV-----------VALNDGAMESTTATRLSSYRLFAEH 89
           S D REEY +AFRTESY +FW RV           V  + G      + RL SYRLFAEH
Sbjct: 31  SFDFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSYRLFAEH 90

Query: 90  LLDPTDPTVKTILSS--AAHLRPNSISLLSDYFSHTANASLFCGRLLKTLDHFRRKLRPF 149
           LL+P    V   L+S   + LRP+   LL+ Y++ TANAS  C  LLK ++H R + RP 
Sbjct: 91  LLEPDQRAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRLRYRPL 150

Query: 150 KQFHH-------------------NPFVQCAEG---LQIIQTGCSKLLKRLEFNRDRARA 209
           K                        PF   A     L+ +Q G   LL+ L+  R +AR 
Sbjct: 151 KHTLRKLASDVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLLRGLDAGRKKARH 210

Query: 210 ERRRVRYFQHGLGGLLVAVTASLTVIVVTHGMALLVA-APGLLVGAVKLGAK--SGKVGR 269
             R V   +  L    V   A + V+    G+ +L A A   ++    LG +  SG+  R
Sbjct: 211 RIRSVARLRRALSVSFVTAVAVVAVVGACIGVHILAAFAAFPMMSPAWLGERFFSGRAAR 270

Query: 270 ----VLDVAAKGTYTLNRDFDTVARVVARVSQEVERMRVLMRFWVERGEDCGRGREP--I 326
                L+ AAKGTY LNRD +T++R+VARV  E E M  L+R  VE     G G +   +
Sbjct: 271 RALVQLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALLRLCVEHRPAAGAGGKGRLV 330

BLAST of Tan0021886 vs. ExPASy Swiss-Prot
Match: Q10RR9 (UPF0496 protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0148000 PE=2 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.5e-45
Identity = 132/340 (38.82%), Postives = 175/340 (51.47%), Query Frame = 0

Query: 30  SVDVREEYANAFRTESYLEFWTRV-----------VALNDGAMESTTATRLSSYRLFAEH 89
           S D REEY +AFRTESY +FW RV           V  + G      + RL SYRLFAEH
Sbjct: 31  SFDFREEYTSAFRTESYNDFWARVLDITLAHGAALVPRHGGGGGCAASKRLPSYRLFAEH 90

Query: 90  LLDPTDPTVKTILSS--AAHLRPNSISLLSDYFSHTANASLFCGRLLKTLDHFRRKLRPF 149
           LL+P    V   L+S   + LRP+   LL+ Y++ TANAS  C  LLK ++H R + RP 
Sbjct: 91  LLEPDQRAVAAALASPRGSRLRPDVRGLLAAYYAETANASFLCSHLLKDIEHIRLRYRPL 150

Query: 150 KQFHH-------------------NPFVQCAEG---LQIIQTGCSKLLKRLEFNRDRARA 209
           K                        PF   A     L+ +Q G   LL+ L+  R +AR 
Sbjct: 151 KHTLRKLASDVGVSGLADVSAALGQPFTALAASQGRLREVQAGSGDLLRGLDAGRKKARH 210

Query: 210 ERRRVRYFQHGLGGLLVAVTASLTVIVVTHGMALLVA-APGLLVGAVKLGAK--SGKVGR 269
             R V   +  L    V   A + V+    G+ +L A A   ++    LG +  SG+  R
Sbjct: 211 RIRSVARLRRALSVSFVTAVAVVAVVGACIGVHILAAFAAFPMMSPAWLGERFFSGRAAR 270

Query: 270 ----VLDVAAKGTYTLNRDFDTVARVVARVSQEVERMRVLMRFWVERGEDCGRGREP--I 326
                L+ AAKGTY LNRD +T++R+VARV  E E M  L R  VE     G G +   +
Sbjct: 271 RALVQLEAAAKGTYILNRDMETISRLVARVRDEGEHMVALRRLCVEHRPAAGAGGKGRLV 330

BLAST of Tan0021886 vs. ExPASy Swiss-Prot
Match: Q6DYE5 (UPF0496 protein At1g20180 OS=Arabidopsis thaliana OX=3702 GN=At1g20180 PE=2 SV=2)

HSP 1 Score: 104.4 bits (259), Expect = 2.6e-21
Identity = 107/396 (27.02%), Postives = 170/396 (42.93%), Query Frame = 0

Query: 3   KSKIVSRLRIFFSCSDRGPPH-IRNHPISVDVREEYANAFRTESYLEFWTRV-------- 62
           K+ + SRL+ F +   +      R+    + V EEY  AFRT SYLE  T+         
Sbjct: 5   KNFLGSRLKSFPTRKGKSDKECCRSLTSKLSVNEEYKEAFRTNSYLETRTKAEDQLGITS 64

Query: 63  -VALNDGAMESTTATRLSSYRLFAEHLLDPTDPTVKTILSSAAHLRPNSISLLSDYFSHT 122
              L+  +   ++++ LS +  F ++LLDP   T+  ++  +     +  +L+  +F  +
Sbjct: 65  CSKLSSSSPSPSSSSDLSFHSHFTDYLLDPPQETLDALMQDS-----SLDNLIVTFFDLS 124

Query: 123 ANASLFCGRLLKTLDHF--------------RRKLRPFKQFHHNPFVQCA---------- 182
           + A   C  LL+ L                 +R     K    +P + CA          
Sbjct: 125 SEACDVCETLLQCLQQIKINHNKIKRVMKIGKRVCNGAKTLECSPEMLCALIFQELSRFA 184

Query: 183 ------------EGLQIIQTGCSKLLKRLEFNRDRARAERRRVRYFQ--HGLGGLLVAVT 242
                          +I+    S LL +L     + R  RR++R+F+    LGG  + +T
Sbjct: 185 ALKNPLCRIVNEAQFRIVHDANSDLLTKL---TSKKRRIRRKIRFFKFCKKLGGYSLVIT 244

Query: 243 ASLTV----IVVTHGMALLVAAPGLL-------------VGAVKLGAKS---GKVGRVLD 302
            S  V    I+  H +  + AAP LL              G +    K     K+G  +D
Sbjct: 245 HSAIVITLLIIALHSILGVFAAPALLGLCSFCLLRKKKAKGRMHKSNKDTTLEKLGTQID 304

Query: 303 VAAKGTYTLNRDFDTVARVVARVSQEVERMRVLMRFWVERGEDCGRGR--EPIGEVARQL 329
           +AAKG + L  D DT++R+  R+  E+E  + +          C + R  E + E  R+ 
Sbjct: 305 IAAKGMFILINDLDTLSRLAGRLCDEIEHRKTVAAM-------CAKSRKIEVLKEALREF 364

BLAST of Tan0021886 vs. ExPASy Swiss-Prot
Match: Q9LJK4 (UPF0496 protein At3g19250 OS=Arabidopsis thaliana OX=3702 GN=At3g19250 PE=2 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.1e-19
Identity = 93/338 (27.51%), Postives = 155/338 (45.86%), Query Frame = 0

Query: 32  DVREEYANAFRTESYLEFWTRVVALNDGAMESTTATRLSSYRLFAEHLLDPTDPTVKTIL 91
           ++  E A+AF+T SY +  +R++ + D   E        +  LF    L P + +V+  L
Sbjct: 35  NLSHELAHAFQTPSYHDIRSRLLVI-DPTQE--------NLELFLSQELRPNNESVQEAL 94

Query: 92  SSAAHLRPNSISLLSDYFSHTANASLFCGRLLKTLDHFRRKL--------RPFKQFHH-- 151
           S     +    +L+S YF H+ +A+ FC  L + +   R  L          F +  H  
Sbjct: 95  SLRHAKQTTLTNLVSTYFQHSEDATRFCLNLYQNVHSARCHLYTPLLDLFNIFPRDSHSA 154

Query: 152 --------------------NPFVQC-AEGLQIIQTGCSKLLKRLEFNRDRARAERRRVR 211
                               NPF    +   Q  Q    +L  +L+    R R  + RVR
Sbjct: 155 IDESFCNLAFDVFLKLDTFENPFASPESHSFQDTQLCFYQLADKLD---TRIRKSKSRVR 214

Query: 212 YFQHGLGGLLVAVTASLTVIVVTHGMALLVAAPGLLVGAVKL-------GAKSGKVGRV- 271
              H   G  + +  ++ V+  +       A P +LV A  L         K  ++  + 
Sbjct: 215 LLHHATAGSALCLVTAVVVVAASAAFIAYHALPTILVVAGPLCTPYLPHSFKKKELSNIF 274

Query: 272 -LDVAAKGTYTLNRDFDTVARVVARVSQEVERMRVLMRFWVERGEDCGRGREPIGEVARQ 330
            L+VAAKGT+ LN+D DT+ R+V+R+   V+  ++L+R  +ERG D       I E  +Q
Sbjct: 275 QLNVAAKGTFALNKDLDTIDRLVSRLHTGVKNDKLLIRLGLERGRDV----YTIPEFVKQ 334

BLAST of Tan0021886 vs. NCBI nr
Match: XP_022159588.1 (UPF0496 protein At3g49070 [Momordica charantia])

HSP 1 Score: 439.1 bits (1128), Expect = 3.4e-119
Identity = 246/379 (64.91%), Postives = 287/379 (75.73%), Query Frame = 0

Query: 1   MKKSKIVSRLRIFFSCSDRGPPHIRNHPISVDVREEYANAFRTESYLEFWTRVVALNDG- 60
           M+K KIVSRL  F SCSD G PHI ++PI VDVREEYANAFRTESY++FWTRV+ LNDG 
Sbjct: 1   MRKFKIVSRLIKFLSCSD-GEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGD 60

Query: 61  -----AMESTTATRLSSYRLFAEHLLDPTDPTVKTILSSAAHLRPNSISLLSDYFSHTAN 120
                 +ESTTATRLSSYRLFAEHLLDPT PTVK +L+S  +LRPNS SLL+DYFSHTAN
Sbjct: 61  LTTQLPVESTTATRLSSYRLFAEHLLDPTQPTVKRLLAS-TYLRPNSYSLLTDYFSHTAN 120

Query: 121 ASLFCGRLLKTLDHFRRKLRPFK---------QFH------------------HNPFVQC 180
           ASL CGRLLK + + R KLRP K         +F+                  HNPFV  
Sbjct: 121 ASLLCGRLLKDIHNLRLKLRPLKITLHSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSS 180

Query: 181 AEG---LQIIQTGCSKLLKRLEFNRDRARAERRRVRYFQHGLGGLLVAVTASLTVIVVTH 240
           A     ++IIQ+GCS LLK+LEFNRD+ARA+ RRVRYFQHG  G LVA+T SLTVIV+TH
Sbjct: 181 APSTRRIRIIQSGCSNLLKQLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTH 240

Query: 241 GMALLVAAPGLLVGAVKLGAKSGKVGR---VLDVAAKGTYTLNRDFDTVARVVARVSQEV 300
           G+AL VAAPGLLV +++L AKS K+ +    L+VAAKGTYTLNRDFDTV R+VAR+S E+
Sbjct: 241 GIALFVAAPGLLVASIEL-AKSRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHEL 300

Query: 301 ERMRVLMRFWVERGEDCGRGREPIGEVARQLKQSCVNFSEQLDELEEHLYLCFMTINRAR 341
           E MRV+ RFW+ER ED    R+ I EVARQLKQ+  +FS+QLDELEEHLYLCFMTINRAR
Sbjct: 301 EHMRVMTRFWLERRED---RRQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRAR 360

BLAST of Tan0021886 vs. NCBI nr
Match: XP_038875828.1 (UPF0496 protein At3g49070-like [Benincasa hispida])

HSP 1 Score: 426.0 bits (1094), Expect = 3.0e-115
Identity = 233/378 (61.64%), Postives = 276/378 (73.02%), Query Frame = 0

Query: 1   MKKSKIVSRLRIFFSCSDRGPPHIRNHPISVDVREEYANAFRTESYLEFWTRVVALNDG- 60
           MKKSKIVS LR F SCSD G PH+ +H + +DVREEYANAFRTESY++FWTRV+ALN+G 
Sbjct: 1   MKKSKIVSCLRKFLSCSD-GEPHVTSHSVDIDVREEYANAFRTESYIDFWTRVLALNNGD 60

Query: 61  ------AMESTTATRLSSYRLFAEHLLDPTDPTVKTILSSAAHLRPNSISLLSDYFSHTA 120
                 ++ESTTAT LSSYRLF EHLLDP   T+K IL+S  HL  NS SLL DYFSHTA
Sbjct: 61  DLTTQLSVESTTATHLSSYRLFVEHLLDPPQSTIKRILTS-PHLGANSCSLLLDYFSHTA 120

Query: 121 NASLFCGRLLKTLDHFRRKLRPF----------KQFHH------------------NPFV 180
           NASL C  LLK ++H R KL P           ++FHH                  NPF+
Sbjct: 121 NASLSCSHLLKHINHLRLKLHPLEITLQSLENKEKFHHESHFKQLLIRLVEFSNTRNPFI 180

Query: 181 QCAEGLQIIQTGCSKLLKRLEFNRDRARAERRRVRYFQHGLGGLLVAVTASLTVIVVTHG 240
              E +QIIQ GCSKLLK+LEF+RD+A+ + +RVRYFQH   G LVAVTASLTVI+VTHG
Sbjct: 181 PSIEQVQIIQNGCSKLLKQLEFSRDKAQTKLKRVRYFQHSSAGFLVAVTASLTVIIVTHG 240

Query: 241 MALLVAAPGLLVGAVKLGAKSGKVGR---VLDVAAKGTYTLNRDFDTVARVVARVSQEVE 300
           +AL VAAPG LVGA+KL  +  K+ +    L++ AKGTYTLNRDFDT+ R+VAR++ E+E
Sbjct: 241 IALFVAAPGFLVGAIKLANQLRKLVKDVARLNITAKGTYTLNRDFDTIGRLVARLNHELE 300

Query: 301 RMRVLMRFWVERGEDCGRGREPIGEVARQLKQSCVNFSEQLDELEEHLYLCFMTINRARN 341
            MRV+ RFW++RGED    R  IGE+ RQL QS VNFS QLDELEEHLYLCFMTINRARN
Sbjct: 301 HMRVMARFWLDRGED---KRRAIGELVRQLNQSHVNFSHQLDELEEHLYLCFMTINRARN 360

BLAST of Tan0021886 vs. NCBI nr
Match: XP_008436996.1 (PREDICTED: UPF0496 protein At3g49070 isoform X1 [Cucumis melo])

HSP 1 Score: 409.8 bits (1052), Expect = 2.2e-110
Identity = 230/377 (61.01%), Postives = 274/377 (72.68%), Query Frame = 0

Query: 1   MKKSKIVSRLRIFFSCSDRGPPHIRNHPISVDVREEYANAFRTESYLEFWTRVVALNDG- 60
           MKK KI+S LR F SCSD G  H+ N+P+  DV EEYANAFRTESY++FWTRVVALN+G 
Sbjct: 1   MKKLKIISCLRKFLSCSD-GEQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGD 60

Query: 61  ------AMESTTATRLSSYRLFAEHLLDPTDPTVKTILSSAAHLRPNSISLLSD-YFSHT 120
                 ++ESTTATRLSSYRLF EHLLDP  PT+K +L+  AHL PNS SLL D YFSHT
Sbjct: 61  NLTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKRMLT--AHLGPNSCSLLLDHYFSHT 120

Query: 121 ANASLFCGRLLKTLDHFRRKLRPFKQ----FHH---------------------NPFV-Q 180
           +NASL C R+LK + H R KLR   Q    F++                     N FV  
Sbjct: 121 SNASLLCSRILKHIVHLRLKLRSLDQNKQEFNYDDSHFKQLLVCLVEFSNNSTPNSFVPY 180

Query: 181 CAEGLQIIQTGCSKLLKRLEFNRDRARAERRRVRYFQHGLGGLLVAVTASLTVIVVTHGM 240
           C E +QIIQ GCSKLLKRLE++RD+A+ + +RVRYFQH   G LVA+TAS T+IVVTHG+
Sbjct: 181 CMEQVQIIQNGCSKLLKRLEYSRDKAQDKLKRVRYFQHSSAGFLVAITASFTIIVVTHGI 240

Query: 241 ALLVAAPGLLVGAVKLGAKSGKVGR---VLDVAAKGTYTLNRDFDTVARVVARVSQEVER 300
           AL VAAPG LVGA+KL  KS K+ +    L+VAAKGTYTLNRDFDT+ R+VAR+S E+E 
Sbjct: 241 ALFVAAPGFLVGAIKLVKKSRKLAKKVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEH 300

Query: 301 MRVLMRFWVERGEDCGRGREPIGEVARQLKQSCVNFSEQLDELEEHLYLCFMTINRARNL 341
           MRV+ +FW++RGED    R  IGE+ RQL QS  NF++QLDELEEHLYLCFMTINRARNL
Sbjct: 301 MRVMTKFWLDRGED---KRWAIGELLRQLNQSHENFNQQLDELEEHLYLCFMTINRARNL 360

BLAST of Tan0021886 vs. NCBI nr
Match: XP_022995692.1 (UPF0496 protein At3g49070 [Cucurbita maxima])

HSP 1 Score: 406.4 bits (1043), Expect = 2.5e-109
Identity = 236/343 (68.80%), Postives = 267/343 (77.84%), Query Frame = 0

Query: 1   MKKSKIVSRLRIFFSCSDRGPPHIRNHPISVDVREEYANAFRTESYLEFWTRVVALNDGA 60
           MKKSK+VSRLR   S SD G  HIR+ PI VDVREEYANAFRTESYL+FWTRVVAL D A
Sbjct: 1   MKKSKLVSRLRKLRSSSD-GGVHIRSRPIGVDVREEYANAFRTESYLDFWTRVVALKDVA 60

Query: 61  MESTTATRLSSYRLFAEHLLDPTDPTVKTILSSAAHLRPNSISLLSDYFSHTANASLFCG 120
           +ESTTA+RLSSYRLFAEHLLDP +PTVK IL + AHLRPNS SLLSDYFSHTANASL C 
Sbjct: 61  VESTTASRLSSYRLFAEHLLDPNEPTVKRIL-NWAHLRPNSKSLLSDYFSHTANASLLCS 120

Query: 121 RLLKTLDHFRRKLR---------PFKQFH-HNPFVQCAEGLQIIQTGCSKLLKRLEFNRD 180
           RLLK ++H R ++           FK    HNPF       +IIQ GC+KLLK+LEFNRD
Sbjct: 121 RLLKDINHLRPEIAIQSLQNPEFNFKPLSIHNPFP------EIIQNGCAKLLKQLEFNRD 180

Query: 181 RARAERRRVRYFQHGLGGLLVAVTASLTVIVVTHGMALLV--AAPGL--LVGAVKLGAKS 240
           +AR + +RVRYFQHG  GLLVAVTASLTVI+VTHG+ L+V  AAP L  LVGAVKL A+ 
Sbjct: 181 KARTKVKRVRYFQHGSAGLLVAVTASLTVILVTHGIGLVVVAAAPCLAGLVGAVKL-ARV 240

Query: 241 GKVGRVLDVAAKGTYTLNRDFDTVARVVARVSQEVERMRVLMRFWVERGEDCGRGREPIG 300
            +V RVLDVAAK TYTLNRDFDTV R+VAR++QEVE MR LMRFWVERGE        +G
Sbjct: 241 KEVARVLDVAAKATYTLNRDFDTVGRLVARLNQEVEHMRGLMRFWVERGEGRDGRHGGVG 300

Query: 301 EVARQLKQSCVNFSEQLDELEEHLYLCFMTINRARNLVVKEIL 330
           EVARQL+Q  +N  + LDELEE LYLCFMTINRARNLV+K+IL
Sbjct: 301 EVARQLQQCRLNLGQHLDELEELLYLCFMTINRARNLVLKQIL 334

BLAST of Tan0021886 vs. NCBI nr
Match: XP_022958040.1 (UPF0496 protein At3g49070-like [Cucurbita moschata])

HSP 1 Score: 406.4 bits (1043), Expect = 2.5e-109
Identity = 234/343 (68.22%), Postives = 265/343 (77.26%), Query Frame = 0

Query: 1   MKKSKIVSRLRIFFSCSDRGPPHIRNHPISVDVREEYANAFRTESYLEFWTRVVALNDGA 60
           MKKSK+VSRLR F S    G  HIR+ PI VDVREEYANAFRTESYL+FWTRVVAL D  
Sbjct: 1   MKKSKLVSRLRTFLS---DGGVHIRSRPIGVDVREEYANAFRTESYLDFWTRVVALKDVV 60

Query: 61  MESTTATRLSSYRLFAEHLLDPTDPTVKTILSSAAHLRPNSISLLSDYFSHTANASLFCG 120
           +ESTTA+RLSSYRLFAEHLLDPT+PTVK IL + AHLRPN  SLLSDYFSHTANASL C 
Sbjct: 61  VESTTASRLSSYRLFAEHLLDPTEPTVKRIL-NWAHLRPNFKSLLSDYFSHTANASLLCS 120

Query: 121 RLLKTLDHFRRKLR---------PFKQFH-HNPFVQCAEGLQIIQTGCSKLLKRLEFNRD 180
           RLLK ++H R ++           FK    HNPF        IIQ GC+KLLK+LEFNRD
Sbjct: 121 RLLKDMNHLRPEIAIQSLQNPEFNFKPLSIHNPFP------AIIQNGCAKLLKQLEFNRD 180

Query: 181 RARAERRRVRYFQHGLGGLLVAVTASLTVIVVTHGMALLV--AAPGL--LVGAVKLGAKS 240
           +AR + +RVRYFQH   GLLVAVTASLTVI+VTHG+ L+V  AAPGL  LVGAVKL A+ 
Sbjct: 181 KARTKVKRVRYFQHSSAGLLVAVTASLTVILVTHGIGLVVVAAAPGLVGLVGAVKL-ARV 240

Query: 241 GKVGRVLDVAAKGTYTLNRDFDTVARVVARVSQEVERMRVLMRFWVERGEDCGRGREPIG 300
            +V RVLDVAAK TYTLNRDFDTV R+VAR++QEVE M+ LMRFWVERGE        +G
Sbjct: 241 KEVARVLDVAAKATYTLNRDFDTVGRLVARLNQEVEHMKGLMRFWVERGEGRDGRHGGVG 300

Query: 301 EVARQLKQSCVNFSEQLDELEEHLYLCFMTINRARNLVVKEIL 330
           EVARQL+Q  +N  + LDELEEHLYLCFMTINRARNLV+K+IL
Sbjct: 301 EVARQLQQCRLNLGQHLDELEEHLYLCFMTINRARNLVLKQIL 332

BLAST of Tan0021886 vs. ExPASy TrEMBL
Match: A0A6J1DZ81 (UPF0496 protein At3g49070 OS=Momordica charantia OX=3673 GN=LOC111025959 PE=3 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 1.7e-119
Identity = 246/379 (64.91%), Postives = 287/379 (75.73%), Query Frame = 0

Query: 1   MKKSKIVSRLRIFFSCSDRGPPHIRNHPISVDVREEYANAFRTESYLEFWTRVVALNDG- 60
           M+K KIVSRL  F SCSD G PHI ++PI VDVREEYANAFRTESY++FWTRV+ LNDG 
Sbjct: 1   MRKFKIVSRLIKFLSCSD-GEPHITSYPIGVDVREEYANAFRTESYIDFWTRVLTLNDGD 60

Query: 61  -----AMESTTATRLSSYRLFAEHLLDPTDPTVKTILSSAAHLRPNSISLLSDYFSHTAN 120
                 +ESTTATRLSSYRLFAEHLLDPT PTVK +L+S  +LRPNS SLL+DYFSHTAN
Sbjct: 61  LTTQLPVESTTATRLSSYRLFAEHLLDPTQPTVKRLLAS-TYLRPNSYSLLTDYFSHTAN 120

Query: 121 ASLFCGRLLKTLDHFRRKLRPFK---------QFH------------------HNPFVQC 180
           ASL CGRLLK + + R KLRP K         +F+                  HNPFV  
Sbjct: 121 ASLLCGRLLKDIHNLRLKLRPLKITLHSIENTRFYHNYNFEPILTCLVEFSNAHNPFVSS 180

Query: 181 AEG---LQIIQTGCSKLLKRLEFNRDRARAERRRVRYFQHGLGGLLVAVTASLTVIVVTH 240
           A     ++IIQ+GCS LLK+LEFNRD+ARA+ RRVRYFQHG  G LVA+T SLTVIV+TH
Sbjct: 181 APSTRRIRIIQSGCSNLLKQLEFNRDKARAKLRRVRYFQHGSAGFLVALTTSLTVIVMTH 240

Query: 241 GMALLVAAPGLLVGAVKLGAKSGKVGR---VLDVAAKGTYTLNRDFDTVARVVARVSQEV 300
           G+AL VAAPGLLV +++L AKS K+ +    L+VAAKGTYTLNRDFDTV R+VAR+S E+
Sbjct: 241 GIALFVAAPGLLVASIEL-AKSRKLAKQVAQLNVAAKGTYTLNRDFDTVGRLVARLSHEL 300

Query: 301 ERMRVLMRFWVERGEDCGRGREPIGEVARQLKQSCVNFSEQLDELEEHLYLCFMTINRAR 341
           E MRV+ RFW+ER ED    R+ I EVARQLKQ+  +FS+QLDELEEHLYLCFMTINRAR
Sbjct: 301 EHMRVMTRFWLERRED---RRQAISEVARQLKQNHASFSQQLDELEEHLYLCFMTINRAR 360

BLAST of Tan0021886 vs. ExPASy TrEMBL
Match: A0A1S3ATJ2 (UPF0496 protein At3g49070 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482559 PE=3 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 1.1e-110
Identity = 230/377 (61.01%), Postives = 274/377 (72.68%), Query Frame = 0

Query: 1   MKKSKIVSRLRIFFSCSDRGPPHIRNHPISVDVREEYANAFRTESYLEFWTRVVALNDG- 60
           MKK KI+S LR F SCSD G  H+ N+P+  DV EEYANAFRTESY++FWTRVVALN+G 
Sbjct: 1   MKKLKIISCLRKFLSCSD-GEQHVTNYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGD 60

Query: 61  ------AMESTTATRLSSYRLFAEHLLDPTDPTVKTILSSAAHLRPNSISLLSD-YFSHT 120
                 ++ESTTATRLSSYRLF EHLLDP  PT+K +L+  AHL PNS SLL D YFSHT
Sbjct: 61  NLTAQVSLESTTATRLSSYRLFVEHLLDPPQPTIKRMLT--AHLGPNSCSLLLDHYFSHT 120

Query: 121 ANASLFCGRLLKTLDHFRRKLRPFKQ----FHH---------------------NPFV-Q 180
           +NASL C R+LK + H R KLR   Q    F++                     N FV  
Sbjct: 121 SNASLLCSRILKHIVHLRLKLRSLDQNKQEFNYDDSHFKQLLVCLVEFSNNSTPNSFVPY 180

Query: 181 CAEGLQIIQTGCSKLLKRLEFNRDRARAERRRVRYFQHGLGGLLVAVTASLTVIVVTHGM 240
           C E +QIIQ GCSKLLKRLE++RD+A+ + +RVRYFQH   G LVA+TAS T+IVVTHG+
Sbjct: 181 CMEQVQIIQNGCSKLLKRLEYSRDKAQDKLKRVRYFQHSSAGFLVAITASFTIIVVTHGI 240

Query: 241 ALLVAAPGLLVGAVKLGAKSGKVGR---VLDVAAKGTYTLNRDFDTVARVVARVSQEVER 300
           AL VAAPG LVGA+KL  KS K+ +    L+VAAKGTYTLNRDFDT+ R+VAR+S E+E 
Sbjct: 241 ALFVAAPGFLVGAIKLVKKSRKLAKKVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEH 300

Query: 301 MRVLMRFWVERGEDCGRGREPIGEVARQLKQSCVNFSEQLDELEEHLYLCFMTINRARNL 341
           MRV+ +FW++RGED    R  IGE+ RQL QS  NF++QLDELEEHLYLCFMTINRARNL
Sbjct: 301 MRVMTKFWLDRGED---KRWAIGELLRQLNQSHENFNQQLDELEEHLYLCFMTINRARNL 360

BLAST of Tan0021886 vs. ExPASy TrEMBL
Match: A0A6J1JZN7 (UPF0496 protein At3g49070 OS=Cucurbita maxima OX=3661 GN=LOC111491151 PE=3 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 1.2e-109
Identity = 236/343 (68.80%), Postives = 267/343 (77.84%), Query Frame = 0

Query: 1   MKKSKIVSRLRIFFSCSDRGPPHIRNHPISVDVREEYANAFRTESYLEFWTRVVALNDGA 60
           MKKSK+VSRLR   S SD G  HIR+ PI VDVREEYANAFRTESYL+FWTRVVAL D A
Sbjct: 1   MKKSKLVSRLRKLRSSSD-GGVHIRSRPIGVDVREEYANAFRTESYLDFWTRVVALKDVA 60

Query: 61  MESTTATRLSSYRLFAEHLLDPTDPTVKTILSSAAHLRPNSISLLSDYFSHTANASLFCG 120
           +ESTTA+RLSSYRLFAEHLLDP +PTVK IL + AHLRPNS SLLSDYFSHTANASL C 
Sbjct: 61  VESTTASRLSSYRLFAEHLLDPNEPTVKRIL-NWAHLRPNSKSLLSDYFSHTANASLLCS 120

Query: 121 RLLKTLDHFRRKLR---------PFKQFH-HNPFVQCAEGLQIIQTGCSKLLKRLEFNRD 180
           RLLK ++H R ++           FK    HNPF       +IIQ GC+KLLK+LEFNRD
Sbjct: 121 RLLKDINHLRPEIAIQSLQNPEFNFKPLSIHNPFP------EIIQNGCAKLLKQLEFNRD 180

Query: 181 RARAERRRVRYFQHGLGGLLVAVTASLTVIVVTHGMALLV--AAPGL--LVGAVKLGAKS 240
           +AR + +RVRYFQHG  GLLVAVTASLTVI+VTHG+ L+V  AAP L  LVGAVKL A+ 
Sbjct: 181 KARTKVKRVRYFQHGSAGLLVAVTASLTVILVTHGIGLVVVAAAPCLAGLVGAVKL-ARV 240

Query: 241 GKVGRVLDVAAKGTYTLNRDFDTVARVVARVSQEVERMRVLMRFWVERGEDCGRGREPIG 300
            +V RVLDVAAK TYTLNRDFDTV R+VAR++QEVE MR LMRFWVERGE        +G
Sbjct: 241 KEVARVLDVAAKATYTLNRDFDTVGRLVARLNQEVEHMRGLMRFWVERGEGRDGRHGGVG 300

Query: 301 EVARQLKQSCVNFSEQLDELEEHLYLCFMTINRARNLVVKEIL 330
           EVARQL+Q  +N  + LDELEE LYLCFMTINRARNLV+K+IL
Sbjct: 301 EVARQLQQCRLNLGQHLDELEELLYLCFMTINRARNLVLKQIL 334

BLAST of Tan0021886 vs. ExPASy TrEMBL
Match: A0A6J1H2B8 (UPF0496 protein At3g49070-like OS=Cucurbita moschata OX=3662 GN=LOC111459391 PE=3 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 1.2e-109
Identity = 234/343 (68.22%), Postives = 265/343 (77.26%), Query Frame = 0

Query: 1   MKKSKIVSRLRIFFSCSDRGPPHIRNHPISVDVREEYANAFRTESYLEFWTRVVALNDGA 60
           MKKSK+VSRLR F S    G  HIR+ PI VDVREEYANAFRTESYL+FWTRVVAL D  
Sbjct: 1   MKKSKLVSRLRTFLS---DGGVHIRSRPIGVDVREEYANAFRTESYLDFWTRVVALKDVV 60

Query: 61  MESTTATRLSSYRLFAEHLLDPTDPTVKTILSSAAHLRPNSISLLSDYFSHTANASLFCG 120
           +ESTTA+RLSSYRLFAEHLLDPT+PTVK IL + AHLRPN  SLLSDYFSHTANASL C 
Sbjct: 61  VESTTASRLSSYRLFAEHLLDPTEPTVKRIL-NWAHLRPNFKSLLSDYFSHTANASLLCS 120

Query: 121 RLLKTLDHFRRKLR---------PFKQFH-HNPFVQCAEGLQIIQTGCSKLLKRLEFNRD 180
           RLLK ++H R ++           FK    HNPF        IIQ GC+KLLK+LEFNRD
Sbjct: 121 RLLKDMNHLRPEIAIQSLQNPEFNFKPLSIHNPFP------AIIQNGCAKLLKQLEFNRD 180

Query: 181 RARAERRRVRYFQHGLGGLLVAVTASLTVIVVTHGMALLV--AAPGL--LVGAVKLGAKS 240
           +AR + +RVRYFQH   GLLVAVTASLTVI+VTHG+ L+V  AAPGL  LVGAVKL A+ 
Sbjct: 181 KARTKVKRVRYFQHSSAGLLVAVTASLTVILVTHGIGLVVVAAAPGLVGLVGAVKL-ARV 240

Query: 241 GKVGRVLDVAAKGTYTLNRDFDTVARVVARVSQEVERMRVLMRFWVERGEDCGRGREPIG 300
            +V RVLDVAAK TYTLNRDFDTV R+VAR++QEVE M+ LMRFWVERGE        +G
Sbjct: 241 KEVARVLDVAAKATYTLNRDFDTVGRLVARLNQEVEHMKGLMRFWVERGEGRDGRHGGVG 300

Query: 301 EVARQLKQSCVNFSEQLDELEEHLYLCFMTINRARNLVVKEIL 330
           EVARQL+Q  +N  + LDELEEHLYLCFMTINRARNLV+K+IL
Sbjct: 301 EVARQLQQCRLNLGQHLDELEEHLYLCFMTINRARNLVLKQIL 332

BLAST of Tan0021886 vs. ExPASy TrEMBL
Match: A0A0A0KN20 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166480 PE=3 SV=1)

HSP 1 Score: 386.7 bits (992), Expect = 9.8e-104
Identity = 216/358 (60.34%), Postives = 258/358 (72.07%), Query Frame = 0

Query: 20  GPPHIRNHPISVDVREEYANAFRTESYLEFWTRVVALNDG-------AMESTTATRLSSY 79
           G  H+ N+P+  DV EEYANAFRTESY++FWTRVVALN+G       ++ESTTATRLSSY
Sbjct: 4   GGQHVINYPVDTDVGEEYANAFRTESYIDFWTRVVALNNGDNLTAQVSLESTTATRLSSY 63

Query: 80  RLFAEHLLDPTDPTVKTILSSAAHLRPNSISLLSD-YFSHTANASLFCGRLLKTLDHFRR 139
           RLF EHLLDP  PT+KTIL+  AHL PNS SLL D YFSHTANASL C R+LK + H R 
Sbjct: 64  RLFVEHLLDPPQPTIKTILT--AHLGPNSCSLLLDHYFSHTANASLLCSRILKQIVHLRL 123

Query: 140 KLRPFKQ----FHH---------------------NPFV-QCAEGLQIIQTGCSKLLKRL 199
           KL    Q    F+H                     N FV  C E +QIIQ GCSKLLKRL
Sbjct: 124 KLHSLDQNKQEFNHDDSHFKQLLVRLFEFSNDSTPNSFVPYCMEQVQIIQNGCSKLLKRL 183

Query: 200 EFNRDRARAERRRVRYFQHGLGGLLVAVTASLTVIVVTHGMALLVAAPGLLVGAVKLGAK 259
           E++RD+ R + +RVRYFQH   G LVA+TAS TVIVVTHG+AL VAAPG LVGA+KL  K
Sbjct: 184 EYSRDKTRDKLKRVRYFQHSSAGFLVAITASFTVIVVTHGIALFVAAPGFLVGAIKLAKK 243

Query: 260 SGKVGR---VLDVAAKGTYTLNRDFDTVARVVARVSQEVERMRVLMRFWVERGEDCGRGR 319
           S K+ +    L+VAAKGTYTLNRDFDT+ R+VAR+S E+E M+V+ +FW+++G   G  R
Sbjct: 244 SRKLAKEVAQLNVAAKGTYTLNRDFDTIGRLVARLSHELEHMKVMAKFWLDKG---GDKR 303

Query: 320 EPIGEVARQLKQSCVNFSEQLDELEEHLYLCFMTINRARNLVVKEILDSGQPVKTPYI 341
             I E+ARQL QS  NF++QLDELEEHLYLCFMTINRARNLVVKEIL+  +P+K  Y+
Sbjct: 304 WAIDELARQLNQSHENFNQQLDELEEHLYLCFMTINRARNLVVKEILNLSKPIKISYL 356

BLAST of Tan0021886 vs. TAIR 10
Match: AT3G49070.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 267.3 bits (682), Expect = 1.7e-71
Identity = 169/368 (45.92%), Postives = 216/368 (58.70%), Query Frame = 0

Query: 5   KIVSRLRIFFSCSDRGPPHIRNHP---ISVDVREEYANAFRTESYLEFWTRVVAL----- 64
           K V++  I    S  G     ++P     VDVREEYANAFRTESY  FWTRVV L     
Sbjct: 40  KFVTKKIIILKLSSIGTASGSSNPKDGADVDVREEYANAFRTESYNHFWTRVVQLSRKKS 99

Query: 65  -------NDGAMESTTATRLSSYRLFAEHLLDPTDPTVKTILSSAAHLRPNSISLLSDYF 124
                  +     S+T+ RL SYRLFA +LLDP   T+  IL   + +  ++ +LLSDYF
Sbjct: 100 AVSSSSSSPPIESSSTSARLMSYRLFAHNLLDPDLNTITRIL-DVSRVGRHTRTLLSDYF 159

Query: 125 SHTANASLFCGRLLKTLDHFRRKLRPFK-QFH---HN----------------PFVQCAE 184
             TANA L C +LLK + H R K    K +FH   HN                PF+    
Sbjct: 160 LETANAFLLCTQLLKNIHHLRSKYESLKPKFHSENHNSLALIDQFTEISKWFDPFISSGS 219

Query: 185 GLQIIQTGCSKLLKRLEFNRDRARAERRRVRYFQHGLGGLLVAVTASLTVIVVTHGMALL 244
            +Q+I++GC  LLKRLE  RD+ RA+ + +    H  G L++A+T +L V + +H  AL 
Sbjct: 220 RIQLIRSGCLYLLKRLESRRDKTRAKLKLINGLTHSSGLLVLALTTTLIVTIASHAFALF 279

Query: 245 VAAPGLLVGAVK---LGAKSGKVGRVLDVAAKGTYTLNRDFDTVARVVARVSQEVERMRV 304
           +AAP LL    K   L  K  K    LDVAAKGTY L+RD DT++R+V R++ EV  +R 
Sbjct: 280 LAAPTLLASQFKPAGLRNKLTKTAARLDVAAKGTYILSRDLDTISRLVTRINDEVNHVRA 339

Query: 305 LMRFWVERGEDCGRGREPIGEVARQLKQSCVNFSEQLDELEEHLYLCFMTINRARNLVVK 335
           +  FWV RG    RG E   EVAR+LK+   +FSE+LDELEEH+YLCFMTINRARNL+VK
Sbjct: 340 MAEFWVGRGSGRVRGSE---EVARELKRCEESFSEELDELEEHIYLCFMTINRARNLLVK 399

BLAST of Tan0021886 vs. TAIR 10
Match: AT1G20180.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 104.4 bits (259), Expect = 1.9e-22
Identity = 107/396 (27.02%), Postives = 170/396 (42.93%), Query Frame = 0

Query: 3   KSKIVSRLRIFFSCSDRGPPH-IRNHPISVDVREEYANAFRTESYLEFWTRV-------- 62
           K+ + SRL+ F +   +      R+    + V EEY  AFRT SYLE  T+         
Sbjct: 5   KNFLGSRLKSFPTRKGKSDKECCRSLTSKLSVNEEYKEAFRTNSYLETRTKAEDQLGITS 64

Query: 63  -VALNDGAMESTTATRLSSYRLFAEHLLDPTDPTVKTILSSAAHLRPNSISLLSDYFSHT 122
              L+  +   ++++ LS +  F ++LLDP   T+  ++  +     +  +L+  +F  +
Sbjct: 65  CSKLSSSSPSPSSSSDLSFHSHFTDYLLDPPQETLDALMQDS-----SLDNLIVTFFDLS 124

Query: 123 ANASLFCGRLLKTLDHF--------------RRKLRPFKQFHHNPFVQCA---------- 182
           + A   C  LL+ L                 +R     K    +P + CA          
Sbjct: 125 SEACDVCETLLQCLQQIKINHNKIKRVMKIGKRVCNGAKTLECSPEMLCALIFQELSRFA 184

Query: 183 ------------EGLQIIQTGCSKLLKRLEFNRDRARAERRRVRYFQ--HGLGGLLVAVT 242
                          +I+    S LL +L     + R  RR++R+F+    LGG  + +T
Sbjct: 185 ALKNPLCRIVNEAQFRIVHDANSDLLTKL---TSKKRRIRRKIRFFKFCKKLGGYSLVIT 244

Query: 243 ASLTV----IVVTHGMALLVAAPGLL-------------VGAVKLGAKS---GKVGRVLD 302
            S  V    I+  H +  + AAP LL              G +    K     K+G  +D
Sbjct: 245 HSAIVITLLIIALHSILGVFAAPALLGLCSFCLLRKKKAKGRMHKSNKDTTLEKLGTQID 304

Query: 303 VAAKGTYTLNRDFDTVARVVARVSQEVERMRVLMRFWVERGEDCGRGR--EPIGEVARQL 329
           +AAKG + L  D DT++R+  R+  E+E  + +          C + R  E + E  R+ 
Sbjct: 305 IAAKGMFILINDLDTLSRLAGRLCDEIEHRKTVAAM-------CAKSRKIEVLKEALREF 364

BLAST of Tan0021886 vs. TAIR 10
Match: AT3G19250.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 99.0 bits (245), Expect = 7.9e-21
Identity = 93/338 (27.51%), Postives = 155/338 (45.86%), Query Frame = 0

Query: 32  DVREEYANAFRTESYLEFWTRVVALNDGAMESTTATRLSSYRLFAEHLLDPTDPTVKTIL 91
           ++  E A+AF+T SY +  +R++ + D   E        +  LF    L P + +V+  L
Sbjct: 35  NLSHELAHAFQTPSYHDIRSRLLVI-DPTQE--------NLELFLSQELRPNNESVQEAL 94

Query: 92  SSAAHLRPNSISLLSDYFSHTANASLFCGRLLKTLDHFRRKL--------RPFKQFHH-- 151
           S     +    +L+S YF H+ +A+ FC  L + +   R  L          F +  H  
Sbjct: 95  SLRHAKQTTLTNLVSTYFQHSEDATRFCLNLYQNVHSARCHLYTPLLDLFNIFPRDSHSA 154

Query: 152 --------------------NPFVQC-AEGLQIIQTGCSKLLKRLEFNRDRARAERRRVR 211
                               NPF    +   Q  Q    +L  +L+    R R  + RVR
Sbjct: 155 IDESFCNLAFDVFLKLDTFENPFASPESHSFQDTQLCFYQLADKLD---TRIRKSKSRVR 214

Query: 212 YFQHGLGGLLVAVTASLTVIVVTHGMALLVAAPGLLVGAVKL-------GAKSGKVGRV- 271
              H   G  + +  ++ V+  +       A P +LV A  L         K  ++  + 
Sbjct: 215 LLHHATAGSALCLVTAVVVVAASAAFIAYHALPTILVVAGPLCTPYLPHSFKKKELSNIF 274

Query: 272 -LDVAAKGTYTLNRDFDTVARVVARVSQEVERMRVLMRFWVERGEDCGRGREPIGEVARQ 330
            L+VAAKGT+ LN+D DT+ R+V+R+   V+  ++L+R  +ERG D       I E  +Q
Sbjct: 275 QLNVAAKGTFALNKDLDTIDRLVSRLHTGVKNDKLLIRLGLERGRDV----YTIPEFVKQ 334

BLAST of Tan0021886 vs. TAIR 10
Match: AT1G20180.2 (Protein of unknown function (DUF677) )

HSP 1 Score: 89.0 bits (219), Expect = 8.1e-18
Identity = 96/363 (26.45%), Postives = 155/363 (42.70%), Query Frame = 0

Query: 3   KSKIVSRLRIFFSCSDRGPPH-IRNHPISVDVREEYANAFRTESYLEFWTRV-------- 62
           K+ + SRL+ F +   +      R+    + V EEY  AFRT SYLE  T+         
Sbjct: 5   KNFLGSRLKSFPTRKGKSDKECCRSLTSKLSVNEEYKEAFRTNSYLETRTKAEDQLGITS 64

Query: 63  -VALNDGAMESTTATRLSSYRLFAEHLLDPTDPTVKTILSSAAHLRPNSISLLSDYFSHT 122
              L+  +   ++++ LS +  F ++LLDP   T+  ++  +     +  +L+  +F  +
Sbjct: 65  CSKLSSSSPSPSSSSDLSFHSHFTDYLLDPPQETLDALMQDS-----SLDNLIVTFFDLS 124

Query: 123 ANASLFCGRLLKTLDHFRRKLRPFKQFHHNPFVQCAEGLQIIQTGCSKLLKRL--EFNRD 182
           + A   C  LL+ L   +      K+        C  G + ++     L   +  E +R 
Sbjct: 125 SEACDVCETLLQCLQQIKINHNKIKRVMKIGKRVC-NGAKTLECSPEMLCALIFQELSRF 184

Query: 183 RAR-------AERRRVRYFQHGLGGLLVAVTASLTVIVVTHGMALLVAAPGLL------- 242
            A            + R        LL  +T+    I     +  + AAP LL       
Sbjct: 185 AALKNPLCRIVNEAQFRIVHDANSDLLTKLTSKKRRIRRKISILGVFAAPALLGLCSFCL 244

Query: 243 ------VGAVKLGAKS---GKVGRVLDVAAKGTYTLNRDFDTVARVVARVSQEVERMRVL 302
                  G +    K     K+G  +D+AAKG + L  D DT++R+  R+  E+E  + +
Sbjct: 245 LRKKKAKGRMHKSNKDTTLEKLGTQIDIAAKGMFILINDLDTLSRLAGRLCDEIEHRKTV 304

Query: 303 MRFWVERGEDCGRGR--EPIGEVARQLKQSCVNFSEQLDELEEHLYLCFMTINRARNLVV 329
                     C + R  E + E  R+       FS+QL ELEEHLYLCF TINR+R LV+
Sbjct: 305 AAM-------CAKSRKIEVLKEALREFNGHEEKFSDQLQELEEHLYLCFHTINRSRRLVL 354

BLAST of Tan0021886 vs. TAIR 10
Match: AT3G19330.1 (Protein of unknown function (DUF677) )

HSP 1 Score: 87.4 bits (215), Expect = 2.4e-17
Identity = 90/339 (26.55%), Postives = 153/339 (45.13%), Query Frame = 0

Query: 32  DVREEYANAFRTESYLEFWTRVVALNDGAMESTTATRLSSYRLFAEHLLDPTDPTVKTIL 91
           ++  E A+AF+T SY +  +RV  + D         +     L ++ L     P  + + 
Sbjct: 46  NLSRELAHAFQTPSYHDVRSRVHVVVDLTQIHHRLIQPDIELLLSQVL----QPNKECVQ 105

Query: 92  SSAAHLRPNSI-SLLSDYFSHTANASLFCGRLLKTLDHFRRKL----------------- 151
            +  H++  ++ +L+S YF H+ +A+  C  L + +   R  L                 
Sbjct: 106 EAIRHVKQTTLTNLVSTYFQHSEDATRLCLNLYQNVHSARHHLYTPLLDLFNIFPGDSLP 165

Query: 152 --------RPFKQF-----HHNPFVQCAEGLQIIQTGCSKLLKRLEFNRD-RARAERRRV 211
                     F  F       NPF    E      T       +L+ N D R R  R RV
Sbjct: 166 AIDESLCDLAFDVFLKLDTFENPF-SSPESYSFRDT--QLCFSQLKHNLDRRLRKSRSRV 225

Query: 212 RYFQHGLGG-------LLVAVTASLTVIVVTHGMALLVAAPGLLVGA-VKLGAKSGKVGR 271
           R   H   G        +VAV AS  V++ +H + +L+   G L    +    K  ++  
Sbjct: 226 RLIHHATAGSSLCLVAAVVAVAAS-AVVIASHALPILLVVAGPLCSPYLPHSFKRKELTN 285

Query: 272 V--LDVAAKGTYTLNRDFDTVARVVARVSQEVERMRVLMRFWVERGEDCGRGREPIGEVA 329
           +  L+ A+KGT+ LN+D DT+ R+V+R+   +E  + L+R  +ERG D       I E+ 
Sbjct: 286 ICQLNAASKGTFVLNKDLDTIDRLVSRLHTGIEYDKFLIRLGLERGRDV----HSIQEIL 345

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SMU42.4e-7045.92UPF0496 protein At3g49070 OS=Arabidopsis thaliana OX=3702 GN=At3g49070 PE=2 SV=1[more]
A2XCJ15.3e-4639.12UPF0496 protein 3 OS=Oryza sativa subsp. indica OX=39946 GN=OsI_009784 PE=3 SV=2[more]
Q10RR94.5e-4538.82UPF0496 protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0148000 PE=2 ... [more]
Q6DYE52.6e-2127.02UPF0496 protein At1g20180 OS=Arabidopsis thaliana OX=3702 GN=At1g20180 PE=2 SV=2[more]
Q9LJK41.1e-1927.51UPF0496 protein At3g19250 OS=Arabidopsis thaliana OX=3702 GN=At3g19250 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_022159588.13.4e-11964.91UPF0496 protein At3g49070 [Momordica charantia][more]
XP_038875828.13.0e-11561.64UPF0496 protein At3g49070-like [Benincasa hispida][more]
XP_008436996.12.2e-11061.01PREDICTED: UPF0496 protein At3g49070 isoform X1 [Cucumis melo][more]
XP_022995692.12.5e-10968.80UPF0496 protein At3g49070 [Cucurbita maxima][more]
XP_022958040.12.5e-10968.22UPF0496 protein At3g49070-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1DZ811.7e-11964.91UPF0496 protein At3g49070 OS=Momordica charantia OX=3673 GN=LOC111025959 PE=3 SV... [more]
A0A1S3ATJ21.1e-11061.01UPF0496 protein At3g49070 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103482559 PE=... [more]
A0A6J1JZN71.2e-10968.80UPF0496 protein At3g49070 OS=Cucurbita maxima OX=3661 GN=LOC111491151 PE=3 SV=1[more]
A0A6J1H2B81.2e-10968.22UPF0496 protein At3g49070-like OS=Cucurbita moschata OX=3662 GN=LOC111459391 PE=... [more]
A0A0A0KN209.8e-10460.34Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G166480 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G49070.11.7e-7145.92Protein of unknown function (DUF677) [more]
AT1G20180.11.9e-2227.02Protein of unknown function (DUF677) [more]
AT3G19250.17.9e-2127.51Protein of unknown function (DUF677) [more]
AT1G20180.28.1e-1826.45Protein of unknown function (DUF677) [more]
AT3G19330.12.4e-1726.55Protein of unknown function (DUF677) [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 289..309
NoneNo IPR availablePANTHERPTHR31113:SF6UPF0496 PROTEIN 3coord: 141..333
coord: 2..138
IPR007749Protein of unknown function DUF677PANTHERPTHR31113UPF0496 PROTEIN 3-RELATEDcoord: 2..138
IPR007749Protein of unknown function DUF677PANTHERPTHR31113UPF0496 PROTEIN 3-RELATEDcoord: 141..333

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0021886.1Tan0021886.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016020 membrane