Tan0021859 (gene) Snake gourd v1

Overview
NameTan0021859
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionheme oxygenase 1, chloroplastic
LocationLG09: 66147423 .. 66150766 (-)
RNA-Seq ExpressionTan0021859
SyntenyTan0021859
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGTTATCCAGACCAGAGGCCCAGAGGTCGATGATGGACACGAAACCCAGTGAGTGTTGCCCCATTCCTCAACCTTGCTTGCTGGCTCCCCTGTCTTCTTCAAATTATTTGGTACCATTAGTTTTCCTCCATTTCCCCTTATAAATAATACTCATAGCTTTTTCTTCAAGATTACGTGAACTCCCAATTCAAGCCAGCCTCCACAAGTCTCTTTCTTCCAATTTCTTCTCCTTCTCTCTTTCAAGGTTTCTTACACTCTTCAATCAACTAACCCACTTCTCGGTTTCTTCAACTATGGCTTCCCTTAACCCAGTTTCCCCCTCACAGTCCCTCTTCAATAAACTTCATTTCAGAGCGCCCGCATCTGTAGCACCCACTTTCTTTTCGAACTCCAATTTGGAATCCAGTTTCTTACCCAAAAGGGTTCTCTCTGCACTTCGGTTTCAGAACAACCCTGCTGGAATTTCAACAGTTCGAATGAAGACCGTGCCCCTTGTTTCGGCCACCACGGCTGAGAAGCCGAAGAAGAGGTACCCTGGTGAATCAAAAGGGTTTGTTGAGGAGATGAGGTTTGTGGCGATGAAATTGCACACGAGGGATCAAGCTAAAGAGGGTGAGAAGGAAACTAAACAGCCCCAAGAGGGCCCTGTCGCTAAATGGGAGCCGACTGTTGAAGGGTATTTGAGATTTCTTGTGGATAGCAAATTGGTTTATGATACGCTGGATAAAATCATTGCCAAGGCTGTATTTCCGTCCTGTAAGTTGTTGCTGATTTCCTGGTTTCTTGGATACGGGTTTTTGGATTTTCCCTGATGTCTTGGATTCAATTTTATGAGCTTGATTCACTCTGGCTTCGCATATGCTGTTCCAATTTTTTTACTTTCTAATGCTTATGCTTTTGTATAGTCAGCCTGTTGCTTTGTGGTTATTGTTTGAAGTTTGGGGGGGTTACAGTGTTTTTGATTTTGTTATTTGACCTTTTTCTCATTTTTGAAATGGTGCACTATTGTCTCATTATAAAACAAAAGTGAACAAAGAAGAAGAGGTGCACTATTGTGCTGTTATTGTTTTTTTTTTTTTTTTTTTAAGAGGAAAGAACTTTCAATAGATTGATATGATTTCTGATTCCCCTTACCAGCCTTTTTGTTCCATGGTTTCTGGAGTGTTGAGAAAGATGGTTGGGGGTATTAGTGGTGTTTTGGTTGATAGGTTACAGGGGACAACAGAAAGCTCTGACTCAGATAGTAAAATTTGAAGATAACCGAATTAATTATGAATGCAAAACTGTGGCATACTTGTTTGAATTTAAAGTCTTATACTAATTTCCATACATTTAATCCACGTTTGATGTGGAGTCTGAGAGATACATTCTTTCCTAGTTGTCCTTTGCATAAATGAATCTGCAGATTATGATCTCCTCACCAGGCATCAGTTTATAATCCTTTAAACACCAAAAGCCATAATTTTTTAATTATTGAGACTGAGGGTCTTTCTAAGGGTCTATTTGAATTGTCTTTTCAAGTGCTTAATTCGCTTTTTTTACACTTGAAAACTATTCTTTAAGCATTTAGAAGGTCATTTCAAACAGACCCTAAGTTAGATTGAAGTGACCTTATTGCAGGTCGGTTGCGTTATTGCTCTCTTTGACTCTCTCTCTTTCTCTTTCCCTCAGAAGTAGAACATCCTTTGTTGTGCACACGGGCGACTACTTAAATTATGTTGAAGGTTATTATGTATCTAGGCTAGATAATATGACAGATTTTTCCTTTCAAGAAATAGTTGGGTGGAGAAATTGATATCTCTAGGTAGATCTATAGATAGATCTGCTTGATCTGAAGGTTGGTTTAAATTGTTTAGTGATCTTTTCGAAAATTAAGCTTTGTGCATGGTTCTTTTTCTAGTTCACGCATAAGTATCACAGGAAAGCTAATTGTAGGATGTTGATGGAGTCTTTTGTATGTGGTAATAGATGCGGAGTTTCGTAACACAGGATTGGAAAGGTCTGAACCATTGGCAAAAGATTTGGAGTGGTTCAAAGAGCAAGGCTATGTCATTCCGGAACCTTCTTCTCCTGGGGTCACCTATGCTCGTTATCTTGAAGAACTATCGGAAAAGGATCCTCAAGCATTTATTTGCCATTTCTATAATACATACTTTGCCCACACTGCTGGTGGTCGGATGATTGGGAGAAAGGTTAGCAATCATTTTCTGGAACAACTTTCTCTGAGCTTTATACTAATATGTATGCAAATTCACCATATGCTCATTTTCATTATCCTGTTGCATAATTGATTCAGTCAACTGCCATGAGTAACTCGATGGTCAATCAGAGCCAATATGATTAATAGTAAAAGGAATGAGTTTAAGTCATGGCAGTTACCTACTTAGGATGTTAGTTTCCTTGGCAACCAATGCAATATCATCATGTGGTTGTATACGTGAGAATAGTCAAGGTGGTTGGCTCCACTACTCACAGATATTAAAAAGAAAAGAAAAGTTTGGTCAGTTTTTTCACTTTTCTTAGGACTTTAGATATATACCAACTTGTATGACCTTGCTGATCTCTTAATTTGGCTCTTTCACAGAATACACCTCTGTATTCTCATGTAGATAATCGACCTGACTGCTGTTACTTGTCATGTAGATAATCTATTGCATAAGCTATTTCGGTGTAAGCCGTGTCGTGTCTTCATGTGGTGTGAGCTCGTCTTAGTCCTACTTTACATTCATCTACTTCAGGTAGCAGAAAAGATACTCGACAATAAGGAGCTGGAATTTTATAAATGGGATGGAGACCTTTCCCAATTGCTGCAGAATGTAAGGGAGAAGCTGAACAAAGTCGCCGAAGTAAGTCACTCTCTTTCTTCCTATGGGCGTCGATTCGCCTTTTTTGTTTTCCAAATGAATTCAGAGAATGTCCTTTTGTTATTCCTGTTGTACGGAACCATGTTGTATGATAGACTGTACTCAATATGATGTTGGTTGTCATGTTTGTTTACCAGAGCTGGAGTAGGGAGGAGAAGGACCATTGCCTGGAGGAAACAGAGAAATCTTTTAAGCACTCGGGGGAAATTCTCCGTTTAATATTGTCATGATCACGACGACGACGACAACGAGAACGAGGACGATGCTTATCTTTTAATTGTCGTGTTATTTTTTTTGTATAGCTCTAATGGGCTATGCCTCCTTTGTCCAGGCTTGAATTCTTGGATGTCCCTTGTTGAAACTTCCACTTTTATTTGAGGATAAAAGTATTTGTTTGTAAACTCTGTAAGTTATGTCCTGAATGGGATATTCAAGTGTATTTATAGGAATCAATTTGGCTATCCTTTAACTGCAAGAAA

mRNA sequence

GAGTTATCCAGACCAGAGGCCCAGAGGTCGATGATGGACACGAAACCCAGTGAGTGTTGCCCCATTCCTCAACCTTGCTTGCTGGCTCCCCTGTCTTCTTCAAATTATTTGGTACCATTAGTTTTCCTCCATTTCCCCTTATAAATAATACTCATAGCTTTTTCTTCAAGATTACGTGAACTCCCAATTCAAGCCAGCCTCCACAAGTCTCTTTCTTCCAATTTCTTCTCCTTCTCTCTTTCAAGGTTTCTTACACTCTTCAATCAACTAACCCACTTCTCGGTTTCTTCAACTATGGCTTCCCTTAACCCAGTTTCCCCCTCACAGTCCCTCTTCAATAAACTTCATTTCAGAGCGCCCGCATCTGTAGCACCCACTTTCTTTTCGAACTCCAATTTGGAATCCAGTTTCTTACCCAAAAGGGTTCTCTCTGCACTTCGGTTTCAGAACAACCCTGCTGGAATTTCAACAGTTCGAATGAAGACCGTGCCCCTTGTTTCGGCCACCACGGCTGAGAAGCCGAAGAAGAGGTACCCTGGTGAATCAAAAGGGTTTGTTGAGGAGATGAGGTTTGTGGCGATGAAATTGCACACGAGGGATCAAGCTAAAGAGGGTGAGAAGGAAACTAAACAGCCCCAAGAGGGCCCTGTCGCTAAATGGGAGCCGACTGTTGAAGGGTATTTGAGATTTCTTGTGGATAGCAAATTGGTTTATGATACGCTGGATAAAATCATTGCCAAGGCTGTATTTCCGTCCTATGCGGAGTTTCGTAACACAGGATTGGAAAGGTCTGAACCATTGGCAAAAGATTTGGAGTGGTTCAAAGAGCAAGGCTATGTCATTCCGGAACCTTCTTCTCCTGGGGTCACCTATGCTCGTTATCTTGAAGAACTATCGGAAAAGGATCCTCAAGCATTTATTTGCCATTTCTATAATACATACTTTGCCCACACTGCTGGTGGTCGGATGATTGGGAGAAAGGTAGCAGAAAAGATACTCGACAATAAGGAGCTGGAATTTTATAAATGGGATGGAGACCTTTCCCAATTGCTGCAGAATGTAAGGGAGAAGCTGAACAAAGTCGCCGAAAGCTGGAGTAGGGAGGAGAAGGACCATTGCCTGGAGGAAACAGAGAAATCTTTTAAGCACTCGGGGGAAATTCTCCGTTTAATATTGTCATGATCACGACGACGACGACAACGAGAACGAGGACGATGCTTATCTTTTAATTGTCGTGTTATTTTTTTTGTATAGCTCTAATGGGCTATGCCTCCTTTGTCCAGGCTTGAATTCTTGGATGTCCCTTGTTGAAACTTCCACTTTTATTTGAGGATAAAAGTATTTGTTTGTAAACTCTGTAAGTTATGTCCTGAATGGGATATTCAAGTGTATTTATAGGAATCAATTTGGCTATCCTTTAACTGCAAGAAA

Coding sequence (CDS)

ATGGCTTCCCTTAACCCAGTTTCCCCCTCACAGTCCCTCTTCAATAAACTTCATTTCAGAGCGCCCGCATCTGTAGCACCCACTTTCTTTTCGAACTCCAATTTGGAATCCAGTTTCTTACCCAAAAGGGTTCTCTCTGCACTTCGGTTTCAGAACAACCCTGCTGGAATTTCAACAGTTCGAATGAAGACCGTGCCCCTTGTTTCGGCCACCACGGCTGAGAAGCCGAAGAAGAGGTACCCTGGTGAATCAAAAGGGTTTGTTGAGGAGATGAGGTTTGTGGCGATGAAATTGCACACGAGGGATCAAGCTAAAGAGGGTGAGAAGGAAACTAAACAGCCCCAAGAGGGCCCTGTCGCTAAATGGGAGCCGACTGTTGAAGGGTATTTGAGATTTCTTGTGGATAGCAAATTGGTTTATGATACGCTGGATAAAATCATTGCCAAGGCTGTATTTCCGTCCTATGCGGAGTTTCGTAACACAGGATTGGAAAGGTCTGAACCATTGGCAAAAGATTTGGAGTGGTTCAAAGAGCAAGGCTATGTCATTCCGGAACCTTCTTCTCCTGGGGTCACCTATGCTCGTTATCTTGAAGAACTATCGGAAAAGGATCCTCAAGCATTTATTTGCCATTTCTATAATACATACTTTGCCCACACTGCTGGTGGTCGGATGATTGGGAGAAAGGTAGCAGAAAAGATACTCGACAATAAGGAGCTGGAATTTTATAAATGGGATGGAGACCTTTCCCAATTGCTGCAGAATGTAAGGGAGAAGCTGAACAAAGTCGCCGAAAGCTGGAGTAGGGAGGAGAAGGACCATTGCCTGGAGGAAACAGAGAAATCTTTTAAGCACTCGGGGGAAATTCTCCGTTTAATATTGTCATGA

Protein sequence

MASLNPVSPSQSLFNKLHFRAPASVAPTFFSNSNLESSFLPKRVLSALRFQNNPAGISTVRMKTVPLVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGPVAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKEQGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNKELEFYKWDGDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS
Homology
BLAST of Tan0021859 vs. ExPASy Swiss-Prot
Match: O48782 (Heme oxygenase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HO1 PE=1 SV=2)

HSP 1 Score: 401.0 bits (1029), Expect = 1.2e-110
Identity = 205/296 (69.26%), Postives = 240/296 (81.08%), Query Frame = 0

Query: 1   MASLNPVSPSQSLFNKLHFRAPASVAPTFFSNSNLESSFLPKRV-LSALRFQNNPAGIST 60
           MA L P+S S S+F          ++   FS+S+    FL  R+ + ++    +P+    
Sbjct: 1   MAYLAPISSSLSIFKN------PQLSRFQFSSSSPNPLFLRPRIQILSMTMNKSPS---- 60

Query: 61  VRMKTVPLVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGPV 120
                V + + T AEK KKRYPGESKGFVEEMRFVAM+LHT+DQAKEGEKETK  +E PV
Sbjct: 61  ----LVVVAATTAAEKQKKRYPGESKGFVEEMRFVAMRLHTKDQAKEGEKETKSIEERPV 120

Query: 121 AKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKEQ 180
           AKWEPTVEGYLRFLVDSKLVYDTL+ II  + FP+YAEF+NTGLER+E L+ DLEWFKEQ
Sbjct: 121 AKWEPTVEGYLRFLVDSKLVYDTLELIIQDSNFPTYAEFKNTGLERAEKLSTDLEWFKEQ 180

Query: 181 GYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNKE 240
           GY IPEP++PG TY++YL+EL+EKDPQAFICHFYN YFAH+AGGRMIGRKVAE+ILDNKE
Sbjct: 181 GYEIPEPTAPGKTYSQYLKELAEKDPQAFICHFYNIYFAHSAGGRMIGRKVAERILDNKE 240

Query: 241 LEFYKWDGDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 296
           LEFYKWDG+LSQLLQNVREKLNKVAE W+REEK+HCLEETEKSFK+SGEILRLILS
Sbjct: 241 LEFYKWDGELSQLLQNVREKLNKVAEEWTREEKNHCLEETEKSFKYSGEILRLILS 282

BLAST of Tan0021859 vs. ExPASy Swiss-Prot
Match: Q9C9L4 (Heme oxygenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HO3 PE=1 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 5.3e-103
Identity = 178/229 (77.73%), Postives = 204/229 (89.08%), Query Frame = 0

Query: 67  LVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGPVAKWEPTV 126
           + +A   EK +K+YPGESKGFVEEMRFVAM+LHT+DQA+EGEKE++ P+EGPVAKWEPTV
Sbjct: 57  VTAAAITEKQQKKYPGESKGFVEEMRFVAMRLHTKDQAREGEKESRSPEEGPVAKWEPTV 116

Query: 127 EGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKEQGYVIPEP 186
           EGYL FLVDSKLVYDTL+ II  + FP+YA F+NTGLER+E L KDLEWFKEQGY IPEP
Sbjct: 117 EGYLHFLVDSKLVYDTLEGIIDGSNFPTYAGFKNTGLERAESLRKDLEWFKEQGYEIPEP 176

Query: 187 SSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNKELEFYKWD 246
            +PG TY+ YL++L+E DPQAFICHFYN YFAH+AGG+MIG KV++KILDNKELEFYKWD
Sbjct: 177 MAPGKTYSEYLKDLAENDPQAFICHFYNIYFAHSAGGQMIGTKVSKKILDNKELEFYKWD 236

Query: 247 GDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 296
           G LSQLLQNVR+KLNKVAE W+REEK HCLEETEKSFK SGEILRLILS
Sbjct: 237 GQLSQLLQNVRQKLNKVAEWWTREEKSHCLEETEKSFKFSGEILRLILS 285

BLAST of Tan0021859 vs. ExPASy Swiss-Prot
Match: Q69XJ4 (Heme oxygenase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=HO1 PE=2 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 4.2e-92
Identity = 165/229 (72.05%), Postives = 192/229 (83.84%), Query Frame = 0

Query: 67  LVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGPVAKWEPTV 126
           + +AT AE        E K FVEEMR VAM+LHT+DQAKEGEKE   PQ  PVA+WEP+V
Sbjct: 64  VAAATAAEMAPAASGEEGKPFVEEMRAVAMRLHTKDQAKEGEKE---PQAPPVARWEPSV 123

Query: 127 EGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKEQGYVIPEP 186
           +GYLRFLVDSKLV++TL+ I+ +A  P YAEFRNTGLERSE L KDLEWFKEQG+ IPEP
Sbjct: 124 DGYLRFLVDSKLVFETLETIVDRAAVPWYAEFRNTGLERSEQLKKDLEWFKEQGHTIPEP 183

Query: 187 SSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNKELEFYKWD 246
           S+PG TYA YLEEL+EKD QAFICHFYN YFAHTAGGRMIG+KV+E IL+ KELEFYKW+
Sbjct: 184 SAPGTTYASYLEELAEKDSQAFICHFYNVYFAHTAGGRMIGKKVSENILNKKELEFYKWE 243

Query: 247 GDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 296
           G+LSQLLQNVR KLN+VA SW+REEKDHCL+ETEKSF +SG++LR I +
Sbjct: 244 GNLSQLLQNVRNKLNEVASSWTREEKDHCLDETEKSFSYSGDLLRHIFT 289

BLAST of Tan0021859 vs. ExPASy Swiss-Prot
Match: Q9LQC0 (Heme oxygenase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HO4 PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 2.7e-83
Identity = 150/228 (65.79%), Postives = 181/228 (79.39%), Query Frame = 0

Query: 68  VSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGPVAKWEPTVE 127
           V A   EKP++RYP E  GFVEEMRFV MK+H RDQ KEG+ ++       V+ W  T+E
Sbjct: 60  VVAAAGEKPERRYPREPNGFVEEMRFVVMKIHPRDQVKEGKSDSND----LVSTWNFTIE 119

Query: 128 GYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKEQGYVIPEPS 187
           GYL+FLVDSKLV++TL++II ++   +YA  +NTGLER+E L++DLEWFKEQGY IPE  
Sbjct: 120 GYLKFLVDSKLVFETLERIINESAIQAYAGLKNTGLERAENLSRDLEWFKEQGYEIPESM 179

Query: 188 SPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNKELEFYKWDG 247
            PG  Y++YL+ ++EKDP AFICHFYN  FAH+AGGRMIG KVAEKILDNKELEFYKWDG
Sbjct: 180 VPGKAYSQYLKNIAEKDPPAFICHFYNINFAHSAGGRMIGTKVAEKILDNKELEFYKWDG 239

Query: 248 DLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 296
            LS+LLQNV E+LNKVAE W+REEK+HCLEETEKSFK   EI R +LS
Sbjct: 240 QLSELLQNVSEELNKVAELWTREEKNHCLEETEKSFKFYWEIFRYLLS 283

BLAST of Tan0021859 vs. ExPASy Swiss-Prot
Match: O48722 (Probable inactive heme oxygenase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HO2 PE=2 SV=2)

HSP 1 Score: 237.7 bits (605), Expect = 1.7e-61
Identity = 116/233 (49.79%), Postives = 165/233 (70.82%), Query Frame = 0

Query: 77  KKRYPGESKGFVEEMRFVAMKLHT------------RDQAKEGEKETKQPQEGPVAK--- 136
           +K+YPGE+ G  EEMRFVAM+L               D  KE E+E +   +    K   
Sbjct: 66  RKQYPGENIGITEEMRFVAMRLRNVNGKKLDLSEDKTDTEKEEEEEEEDDDDDDEVKEET 125

Query: 137 WEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKEQGY 196
           W+P+ EG+L++LVDSKLV+DT+++I+ ++   SYA FR TGLER E + KDL+W +EQ  
Sbjct: 126 WKPSKEGFLKYLVDSKLVFDTIERIVDESENVSYAYFRRTGLERCESIEKDLQWLREQDL 185

Query: 197 VIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNKELE 256
           VIPEPS+ GV+YA+YLEE + +    F+ HFY+ YF+H AGG+++ R+V+EK+L+ KELE
Sbjct: 186 VIPEPSNVGVSYAKYLEEQAGESAPLFLSHFYSIYFSHIAGGQVLVRQVSEKLLEGKELE 245

Query: 257 FYKWDGDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLIL 295
           F +W+GD   LL+ VREKLN + E WSR+EK+ CL+ET K+FK+ G+I+RLI+
Sbjct: 246 FNRWEGDAQDLLKGVREKLNVLGEHWSRDEKNKCLKETAKAFKYMGQIVRLII 298

BLAST of Tan0021859 vs. NCBI nr
Match: XP_023007741.1 (heme oxygenase 1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 550.8 bits (1418), Expect = 7.0e-153
Identity = 278/297 (93.60%), Postives = 285/297 (95.96%), Query Frame = 0

Query: 1   MASLNPVSPSQSLFNKLHFRAPASVAPTFFSNSN--LESSFLPKRVLSALRFQNNPAGIS 60
           MASLNPVSPSQSLFNKLHFR P  VAPT FSNSN  LESSFLPKRV SALRF+ + AGIS
Sbjct: 1   MASLNPVSPSQSLFNKLHFRTPTFVAPTSFSNSNSSLESSFLPKRVPSALRFKKSTAGIS 60

Query: 61  TVRMKTVPLVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGP 120
           TVRMKTVPLVSATTAEK KKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETK+PQE P
Sbjct: 61  TVRMKTVPLVSATTAEKSKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKEPQEAP 120

Query: 121 VAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKE 180
           VAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFR+TGLERSE LAKDLEWFKE
Sbjct: 121 VAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRDTGLERSEALAKDLEWFKE 180

Query: 181 QGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNK 240
           QGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAE+IL+NK
Sbjct: 181 QGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAERILENK 240

Query: 241 ELEFYKWDGDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 296
           ELEFYKWDGDLSQLLQNVR+KLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS
Sbjct: 241 ELEFYKWDGDLSQLLQNVRDKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 297

BLAST of Tan0021859 vs. NCBI nr
Match: XP_022933640.1 (heme oxygenase 1, chloroplastic-like [Cucurbita moschata] >KAG7021787.1 Heme oxygenase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 542.3 bits (1396), Expect = 2.5e-150
Identity = 274/297 (92.26%), Postives = 282/297 (94.95%), Query Frame = 0

Query: 1   MASLNPVSPSQSLFNKLHFRAPASVAPTFFSNSN--LESSFLPKRVLSALRFQNNPAGIS 60
           M SLNPVSPSQSLFNKL FR P  VAPT FSNSN  LESSFLP+RV SALRF+ + AGIS
Sbjct: 1   MPSLNPVSPSQSLFNKLRFRTPTFVAPTSFSNSNSSLESSFLPRRVPSALRFKKSTAGIS 60

Query: 61  TVRMKTVPLVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGP 120
           TVRMKTVPLVSAT AEK KKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETK+PQE P
Sbjct: 61  TVRMKTVPLVSATMAEKSKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKEPQEAP 120

Query: 121 VAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKE 180
           VAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFR+TGLERSE LAKDLEWFKE
Sbjct: 121 VAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRDTGLERSEALAKDLEWFKE 180

Query: 181 QGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNK 240
           QGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAE+IL+NK
Sbjct: 181 QGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAERILENK 240

Query: 241 ELEFYKWDGDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 296
           ELEFYKWDGDLSQLLQNVR+KLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS
Sbjct: 241 ELEFYKWDGDLSQLLQNVRDKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 297

BLAST of Tan0021859 vs. NCBI nr
Match: KAG6587901.1 (Heme oxygenase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 536.2 bits (1380), Expect = 1.8e-148
Identity = 270/295 (91.53%), Postives = 279/295 (94.58%), Query Frame = 0

Query: 1   MASLNPVSPSQSLFNKLHFRAPASVAPTFFSNSN--LESSFLPKRVLSALRFQNNPAGIS 60
           M SLNPVSPSQSLFNKL FR P  VAPT FSNSN  LESSFLP+RV SALRF+ + AGIS
Sbjct: 1   MPSLNPVSPSQSLFNKLRFRTPTFVAPTSFSNSNSSLESSFLPRRVPSALRFKKSTAGIS 60

Query: 61  TVRMKTVPLVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGP 120
           TVRMKTVPLVSAT AEK KKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETK+PQE P
Sbjct: 61  TVRMKTVPLVSATMAEKSKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKEPQEAP 120

Query: 121 VAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKE 180
           VAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFR+TGLERSE LAKDLEWFKE
Sbjct: 121 VAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRDTGLERSEALAKDLEWFKE 180

Query: 181 QGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNK 240
           QGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAE+IL+NK
Sbjct: 181 QGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAERILENK 240

Query: 241 ELEFYKWDGDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLI 294
           ELEFYKWDGDLSQLLQNVR+KLNKVAESWSREEKDHCLEETEKSFKHSGEIL L+
Sbjct: 241 ELEFYKWDGDLSQLLQNVRDKLNKVAESWSREEKDHCLEETEKSFKHSGEILLLV 295

BLAST of Tan0021859 vs. NCBI nr
Match: XP_023531699.1 (heme oxygenase 1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 529.3 bits (1362), Expect = 2.2e-146
Identity = 268/295 (90.85%), Postives = 279/295 (94.58%), Query Frame = 0

Query: 1   MASLNPVSPSQSLFNKLHFRAPASVAPTFFSNSNLESSFLPKRVLSALRFQNNPAGISTV 60
           MASLNPVSPSQSLFNKL FR P S +    SNS+LESSFLP+RV SAL  + + AGISTV
Sbjct: 1   MASLNPVSPSQSLFNKLRFRTPTSFSN---SNSSLESSFLPRRVPSALLCKKSTAGISTV 60

Query: 61  RMKTVPLVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGPVA 120
           RMKTVPLVSATTAEK KKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETK+PQE PVA
Sbjct: 61  RMKTVPLVSATTAEKSKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKEPQEAPVA 120

Query: 121 KWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKEQG 180
           KWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFR+TGLERSE LAKDLEWFKEQG
Sbjct: 121 KWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRDTGLERSEALAKDLEWFKEQG 180

Query: 181 YVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNKEL 240
           YVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAE+IL+NKEL
Sbjct: 181 YVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAERILENKEL 240

Query: 241 EFYKWDGDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 296
           EFYKWDGDLSQLLQNVR+KLNKVAESWSREEK+HCLEETEKSFKHSGEILRLILS
Sbjct: 241 EFYKWDGDLSQLLQNVRDKLNKVAESWSREEKNHCLEETEKSFKHSGEILRLILS 292

BLAST of Tan0021859 vs. NCBI nr
Match: XP_022145036.1 (heme oxygenase 1, chloroplastic [Momordica charantia])

HSP 1 Score: 528.9 bits (1361), Expect = 2.9e-146
Identity = 263/297 (88.55%), Postives = 283/297 (95.29%), Query Frame = 0

Query: 1   MASLNPVSPSQSLFNKLHFRAPASVAPTFF--SNSNLESSFLPKRVLSALRFQNNPAGIS 60
           MASLNP+SPSQSLFNK+HFRAPASVAPT F  ++SNLES+F  KR  SA+RF+ NPAGIS
Sbjct: 1   MASLNPISPSQSLFNKIHFRAPASVAPTSFASASSNLESNFFSKRPPSAVRFR-NPAGIS 60

Query: 61  TVRMKTVPLVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGP 120
           TVRMKT+PLVSATTAEKPKKRYPGE+KGFVEEMRFVAMKLHTRDQAKEGEKETK+PQEGP
Sbjct: 61  TVRMKTLPLVSATTAEKPKKRYPGEAKGFVEEMRFVAMKLHTRDQAKEGEKETKEPQEGP 120

Query: 121 VAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKE 180
           VAKWEP+V+GYLRFLVD+KLVYDTLD+IIAKA FPSYAEF NTGLERS+PLAKDL WFKE
Sbjct: 121 VAKWEPSVDGYLRFLVDNKLVYDTLDRIIAKAAFPSYAEFSNTGLERSKPLAKDLGWFKE 180

Query: 181 QGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNK 240
           QGY IPEPSSPG+TYARYLEELSE DPQAFICHFYN YFAHTAGGRMIGRKVAEKIL+NK
Sbjct: 181 QGYAIPEPSSPGITYARYLEELSENDPQAFICHFYNIYFAHTAGGRMIGRKVAEKILNNK 240

Query: 241 ELEFYKWDGDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 296
           ELEFYKWDGDLSQLLQNVREKLNKVAESWSREEK+HCLEETEKSFK+SG+ILRLILS
Sbjct: 241 ELEFYKWDGDLSQLLQNVREKLNKVAESWSREEKNHCLEETEKSFKYSGDILRLILS 296

BLAST of Tan0021859 vs. ExPASy TrEMBL
Match: A0A6J1L1I5 (heme oxygenase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111500260 PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.4e-153
Identity = 278/297 (93.60%), Postives = 285/297 (95.96%), Query Frame = 0

Query: 1   MASLNPVSPSQSLFNKLHFRAPASVAPTFFSNSN--LESSFLPKRVLSALRFQNNPAGIS 60
           MASLNPVSPSQSLFNKLHFR P  VAPT FSNSN  LESSFLPKRV SALRF+ + AGIS
Sbjct: 1   MASLNPVSPSQSLFNKLHFRTPTFVAPTSFSNSNSSLESSFLPKRVPSALRFKKSTAGIS 60

Query: 61  TVRMKTVPLVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGP 120
           TVRMKTVPLVSATTAEK KKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETK+PQE P
Sbjct: 61  TVRMKTVPLVSATTAEKSKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKEPQEAP 120

Query: 121 VAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKE 180
           VAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFR+TGLERSE LAKDLEWFKE
Sbjct: 121 VAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRDTGLERSEALAKDLEWFKE 180

Query: 181 QGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNK 240
           QGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAE+IL+NK
Sbjct: 181 QGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAERILENK 240

Query: 241 ELEFYKWDGDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 296
           ELEFYKWDGDLSQLLQNVR+KLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS
Sbjct: 241 ELEFYKWDGDLSQLLQNVRDKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 297

BLAST of Tan0021859 vs. ExPASy TrEMBL
Match: A0A6J1F5E5 (heme oxygenase 1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111441000 PE=4 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 1.2e-150
Identity = 274/297 (92.26%), Postives = 282/297 (94.95%), Query Frame = 0

Query: 1   MASLNPVSPSQSLFNKLHFRAPASVAPTFFSNSN--LESSFLPKRVLSALRFQNNPAGIS 60
           M SLNPVSPSQSLFNKL FR P  VAPT FSNSN  LESSFLP+RV SALRF+ + AGIS
Sbjct: 1   MPSLNPVSPSQSLFNKLRFRTPTFVAPTSFSNSNSSLESSFLPRRVPSALRFKKSTAGIS 60

Query: 61  TVRMKTVPLVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGP 120
           TVRMKTVPLVSAT AEK KKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETK+PQE P
Sbjct: 61  TVRMKTVPLVSATMAEKSKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKEPQEAP 120

Query: 121 VAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKE 180
           VAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFR+TGLERSE LAKDLEWFKE
Sbjct: 121 VAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRDTGLERSEALAKDLEWFKE 180

Query: 181 QGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNK 240
           QGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAE+IL+NK
Sbjct: 181 QGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAERILENK 240

Query: 241 ELEFYKWDGDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 296
           ELEFYKWDGDLSQLLQNVR+KLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS
Sbjct: 241 ELEFYKWDGDLSQLLQNVRDKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 297

BLAST of Tan0021859 vs. ExPASy TrEMBL
Match: A0A6J1CV64 (heme oxygenase 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111014559 PE=4 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 1.4e-146
Identity = 263/297 (88.55%), Postives = 283/297 (95.29%), Query Frame = 0

Query: 1   MASLNPVSPSQSLFNKLHFRAPASVAPTFF--SNSNLESSFLPKRVLSALRFQNNPAGIS 60
           MASLNP+SPSQSLFNK+HFRAPASVAPT F  ++SNLES+F  KR  SA+RF+ NPAGIS
Sbjct: 1   MASLNPISPSQSLFNKIHFRAPASVAPTSFASASSNLESNFFSKRPPSAVRFR-NPAGIS 60

Query: 61  TVRMKTVPLVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGP 120
           TVRMKT+PLVSATTAEKPKKRYPGE+KGFVEEMRFVAMKLHTRDQAKEGEKETK+PQEGP
Sbjct: 61  TVRMKTLPLVSATTAEKPKKRYPGEAKGFVEEMRFVAMKLHTRDQAKEGEKETKEPQEGP 120

Query: 121 VAKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKE 180
           VAKWEP+V+GYLRFLVD+KLVYDTLD+IIAKA FPSYAEF NTGLERS+PLAKDL WFKE
Sbjct: 121 VAKWEPSVDGYLRFLVDNKLVYDTLDRIIAKAAFPSYAEFSNTGLERSKPLAKDLGWFKE 180

Query: 181 QGYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNK 240
           QGY IPEPSSPG+TYARYLEELSE DPQAFICHFYN YFAHTAGGRMIGRKVAEKIL+NK
Sbjct: 181 QGYAIPEPSSPGITYARYLEELSENDPQAFICHFYNIYFAHTAGGRMIGRKVAEKILNNK 240

Query: 241 ELEFYKWDGDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 296
           ELEFYKWDGDLSQLLQNVREKLNKVAESWSREEK+HCLEETEKSFK+SG+ILRLILS
Sbjct: 241 ELEFYKWDGDLSQLLQNVREKLNKVAESWSREEKNHCLEETEKSFKYSGDILRLILS 296

BLAST of Tan0021859 vs. ExPASy TrEMBL
Match: A0A1S3B8X3 (heme oxygenase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487455 PE=4 SV=1)

HSP 1 Score: 515.8 bits (1327), Expect = 1.2e-142
Identity = 260/296 (87.84%), Postives = 271/296 (91.55%), Query Frame = 0

Query: 1   MASLNPVSPSQSLFNKLHFRAPASVAPTFFSNSNLESSFLP-KRVLSALRFQNNPAGIST 60
           MAS+N +SPSQSLF    F     V PT FSN NL+S FLP KRV  +LRFQ NP GIST
Sbjct: 1   MASINSISPSQSLFKNTLF-----VIPTSFSNLNLDSCFLPNKRVPISLRFQKNPVGIST 60

Query: 61  VRMKTVPLVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGPV 120
           VRM+TVP VSATTAEKP+KRYPGESKGFVEEMRFVAMKLHTRDQAKEGEK TK+PQEGPV
Sbjct: 61  VRMRTVPFVSATTAEKPQKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKVTKEPQEGPV 120

Query: 121 AKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKEQ 180
            KWEPTVEGYL+FLVDSKLVYDTLD+IIAKAVFPSYAEF NTGLERSEPLAKDLEWFKEQ
Sbjct: 121 GKWEPTVEGYLKFLVDSKLVYDTLDRIIAKAVFPSYAEFSNTGLERSEPLAKDLEWFKEQ 180

Query: 181 GYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNKE 240
           GY IPEPSSPGVTYARYLEELSEKDPQAFICHFYN YFAHTAGGRMIGRKVAEKILDNKE
Sbjct: 181 GYAIPEPSSPGVTYARYLEELSEKDPQAFICHFYNIYFAHTAGGRMIGRKVAEKILDNKE 240

Query: 241 LEFYKWDGDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 296
           LEFYKWDGDLSQ+LQNVREKLNKVAESWSREEK+HCLEETEKSFKHSGEILRLILS
Sbjct: 241 LEFYKWDGDLSQMLQNVREKLNKVAESWSREEKNHCLEETEKSFKHSGEILRLILS 291

BLAST of Tan0021859 vs. ExPASy TrEMBL
Match: E2JEI2 (Chloroplast heme oxygenase-1 OS=Cucumis sativus OX=3659 GN=HO1 PE=2 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 1.9e-140
Identity = 256/296 (86.49%), Postives = 269/296 (90.88%), Query Frame = 0

Query: 1   MASLNPVSPSQSLFNKLHFRAPASVAPTFFSNSNLESSFLP-KRVLSALRFQNNPAGIST 60
           MAS+N +SPSQSL     F     V PT FSN N +S FLP KRV  +LRFQ NP GIST
Sbjct: 1   MASINSLSPSQSLLKNTLF-----VIPTSFSNPNFDSCFLPNKRVPFSLRFQKNPVGIST 60

Query: 61  VRMKTVPLVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGPV 120
           VRM+TVP VSATTAEKP+KRYPGESKGFVEEMRFVAMKLHTRDQAKEGEK TK+PQEGPV
Sbjct: 61  VRMRTVPFVSATTAEKPQKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKVTKEPQEGPV 120

Query: 121 AKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKEQ 180
           AKWEPTVEGYL+FLVDSKLVYDTLD+I+AKA FPSYAEF +TGLERSEPLAKDLEWFKEQ
Sbjct: 121 AKWEPTVEGYLKFLVDSKLVYDTLDRIMAKAAFPSYAEFSSTGLERSEPLAKDLEWFKEQ 180

Query: 181 GYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNKE 240
           GY IPEPSSPGVTYARYLEELSEKDPQAFICHFYN YFAHTAGGRMIGRKVAEKILDNKE
Sbjct: 181 GYAIPEPSSPGVTYARYLEELSEKDPQAFICHFYNIYFAHTAGGRMIGRKVAEKILDNKE 240

Query: 241 LEFYKWDGDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 296
           LEFYKWDGDLSQ+LQNVREKLNKVAESWSREEK+HCLEETEKSFKHSGEILRLILS
Sbjct: 241 LEFYKWDGDLSQILQNVREKLNKVAESWSREEKNHCLEETEKSFKHSGEILRLILS 291

BLAST of Tan0021859 vs. TAIR 10
Match: AT2G26670.1 (Plant haem oxygenase (decyclizing) family protein )

HSP 1 Score: 401.0 bits (1029), Expect = 8.4e-112
Identity = 205/296 (69.26%), Postives = 240/296 (81.08%), Query Frame = 0

Query: 1   MASLNPVSPSQSLFNKLHFRAPASVAPTFFSNSNLESSFLPKRV-LSALRFQNNPAGIST 60
           MA L P+S S S+F          ++   FS+S+    FL  R+ + ++    +P+    
Sbjct: 1   MAYLAPISSSLSIFKN------PQLSRFQFSSSSPNPLFLRPRIQILSMTMNKSPS---- 60

Query: 61  VRMKTVPLVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGPV 120
                V + + T AEK KKRYPGESKGFVEEMRFVAM+LHT+DQAKEGEKETK  +E PV
Sbjct: 61  ----LVVVAATTAAEKQKKRYPGESKGFVEEMRFVAMRLHTKDQAKEGEKETKSIEERPV 120

Query: 121 AKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKEQ 180
           AKWEPTVEGYLRFLVDSKLVYDTL+ II  + FP+YAEF+NTGLER+E L+ DLEWFKEQ
Sbjct: 121 AKWEPTVEGYLRFLVDSKLVYDTLELIIQDSNFPTYAEFKNTGLERAEKLSTDLEWFKEQ 180

Query: 181 GYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNKE 240
           GY IPEP++PG TY++YL+EL+EKDPQAFICHFYN YFAH+AGGRMIGRKVAE+ILDNKE
Sbjct: 181 GYEIPEPTAPGKTYSQYLKELAEKDPQAFICHFYNIYFAHSAGGRMIGRKVAERILDNKE 240

Query: 241 LEFYKWDGDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 296
           LEFYKWDG+LSQLLQNVREKLNKVAE W+REEK+HCLEETEKSFK+SGEILRLILS
Sbjct: 241 LEFYKWDGELSQLLQNVREKLNKVAEEWTREEKNHCLEETEKSFKYSGEILRLILS 282

BLAST of Tan0021859 vs. TAIR 10
Match: AT1G69720.1 (heme oxygenase 3 )

HSP 1 Score: 375.6 bits (963), Expect = 3.8e-104
Identity = 178/229 (77.73%), Postives = 204/229 (89.08%), Query Frame = 0

Query: 67  LVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGPVAKWEPTV 126
           + +A   EK +K+YPGESKGFVEEMRFVAM+LHT+DQA+EGEKE++ P+EGPVAKWEPTV
Sbjct: 57  VTAAAITEKQQKKYPGESKGFVEEMRFVAMRLHTKDQAREGEKESRSPEEGPVAKWEPTV 116

Query: 127 EGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKEQGYVIPEP 186
           EGYL FLVDSKLVYDTL+ II  + FP+YA F+NTGLER+E L KDLEWFKEQGY IPEP
Sbjct: 117 EGYLHFLVDSKLVYDTLEGIIDGSNFPTYAGFKNTGLERAESLRKDLEWFKEQGYEIPEP 176

Query: 187 SSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNKELEFYKWD 246
            +PG TY+ YL++L+E DPQAFICHFYN YFAH+AGG+MIG KV++KILDNKELEFYKWD
Sbjct: 177 MAPGKTYSEYLKDLAENDPQAFICHFYNIYFAHSAGGQMIGTKVSKKILDNKELEFYKWD 236

Query: 247 GDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 296
           G LSQLLQNVR+KLNKVAE W+REEK HCLEETEKSFK SGEILRLILS
Sbjct: 237 GQLSQLLQNVRQKLNKVAEWWTREEKSHCLEETEKSFKFSGEILRLILS 285

BLAST of Tan0021859 vs. TAIR 10
Match: AT1G58300.1 (heme oxygenase 4 )

HSP 1 Score: 310.1 bits (793), Expect = 1.9e-84
Identity = 150/228 (65.79%), Postives = 181/228 (79.39%), Query Frame = 0

Query: 68  VSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGPVAKWEPTVE 127
           V A   EKP++RYP E  GFVEEMRFV MK+H RDQ KEG+ ++       V+ W  T+E
Sbjct: 60  VVAAAGEKPERRYPREPNGFVEEMRFVVMKIHPRDQVKEGKSDSND----LVSTWNFTIE 119

Query: 128 GYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKEQGYVIPEPS 187
           GYL+FLVDSKLV++TL++II ++   +YA  +NTGLER+E L++DLEWFKEQGY IPE  
Sbjct: 120 GYLKFLVDSKLVFETLERIINESAIQAYAGLKNTGLERAENLSRDLEWFKEQGYEIPESM 179

Query: 188 SPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNKELEFYKWDG 247
            PG  Y++YL+ ++EKDP AFICHFYN  FAH+AGGRMIG KVAEKILDNKELEFYKWDG
Sbjct: 180 VPGKAYSQYLKNIAEKDPPAFICHFYNINFAHSAGGRMIGTKVAEKILDNKELEFYKWDG 239

Query: 248 DLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 296
            LS+LLQNV E+LNKVAE W+REEK+HCLEETEKSFK   EI R +LS
Sbjct: 240 QLSELLQNVSEELNKVAELWTREEKNHCLEETEKSFKFYWEIFRYLLS 283

BLAST of Tan0021859 vs. TAIR 10
Match: AT2G26670.2 (Plant haem oxygenase (decyclizing) family protein )

HSP 1 Score: 303.5 bits (776), Expect = 1.8e-82
Identity = 167/296 (56.42%), Postives = 198/296 (66.89%), Query Frame = 0

Query: 1   MASLNPVSPSQSLFNKLHFRAPASVAPTFFSNSNLESSFLPKRV-LSALRFQNNPAGIST 60
           MA L P+S S S+F          ++   FS+S+    FL  R+ + ++    +P+    
Sbjct: 1   MAYLAPISSSLSIFKN------PQLSRFQFSSSSPNPLFLRPRIQILSMTMNKSPS---- 60

Query: 61  VRMKTVPLVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGPV 120
                V + + T AEK KKRYPGESKGFVEEMRFVAM+LHT+DQAKE             
Sbjct: 61  ----LVVVAATTAAEKQKKRYPGESKGFVEEMRFVAMRLHTKDQAKED------------ 120

Query: 121 AKWEPTVEGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKEQ 180
                                               AEF+NTGLER+E L+ DLEWFKEQ
Sbjct: 121 ------------------------------------AEFKNTGLERAEKLSTDLEWFKEQ 180

Query: 181 GYVIPEPSSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKVAEKILDNKE 240
           GY IPEP++PG TY++YL+EL+EKDPQAFICHFYN YFAH+AGGRMIGRKVAE+ILDNKE
Sbjct: 181 GYEIPEPTAPGKTYSQYLKELAEKDPQAFICHFYNIYFAHSAGGRMIGRKVAERILDNKE 234

Query: 241 LEFYKWDGDLSQLLQNVREKLNKVAESWSREEKDHCLEETEKSFKHSGEILRLILS 296
           LEFYKWDG+LSQLLQNVREKLNKVAE W+REEK+HCLEETEKSFK+SGEILRLILS
Sbjct: 241 LEFYKWDGELSQLLQNVREKLNKVAEEWTREEKNHCLEETEKSFKYSGEILRLILS 234

BLAST of Tan0021859 vs. TAIR 10
Match: AT1G69720.2 (heme oxygenase 3 )

HSP 1 Score: 261.9 bits (668), Expect = 6.1e-70
Identity = 120/164 (73.17%), Postives = 143/164 (87.20%), Query Frame = 0

Query: 67  LVSATTAEKPKKRYPGESKGFVEEMRFVAMKLHTRDQAKEGEKETKQPQEGPVAKWEPTV 126
           + +A   EK +K+YPGESKGFVEEMRFVAM+LHT+DQA+EGEKE++ P+EGPVAKWEPTV
Sbjct: 57  VTAAAITEKQQKKYPGESKGFVEEMRFVAMRLHTKDQAREGEKESRSPEEGPVAKWEPTV 116

Query: 127 EGYLRFLVDSKLVYDTLDKIIAKAVFPSYAEFRNTGLERSEPLAKDLEWFKEQGYVIPEP 186
           EGYL FLVDSKLVYDTL+ II  + FP+YA F+NTGLER+E L KDLEWFKEQGY IPEP
Sbjct: 117 EGYLHFLVDSKLVYDTLEGIIDGSNFPTYAGFKNTGLERAESLRKDLEWFKEQGYEIPEP 176

Query: 187 SSPGVTYARYLEELSEKDPQAFICHFYNTYFAHTAGGRMIGRKV 231
            +PG TY+ YL++L+E DPQAFICHFYN YFAH+AGG+MIG K+
Sbjct: 177 MAPGKTYSEYLKDLAENDPQAFICHFYNIYFAHSAGGQMIGTKL 220

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O487821.2e-11069.26Heme oxygenase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HO1 PE=1 SV=2[more]
Q9C9L45.3e-10377.73Heme oxygenase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HO3 PE=1 SV=1[more]
Q69XJ44.2e-9272.05Heme oxygenase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=HO1 ... [more]
Q9LQC02.7e-8365.79Heme oxygenase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=HO4 PE=1 SV=1[more]
O487221.7e-6149.79Probable inactive heme oxygenase 2, chloroplastic OS=Arabidopsis thaliana OX=370... [more]
Match NameE-valueIdentityDescription
XP_023007741.17.0e-15393.60heme oxygenase 1, chloroplastic [Cucurbita maxima][more]
XP_022933640.12.5e-15092.26heme oxygenase 1, chloroplastic-like [Cucurbita moschata] >KAG7021787.1 Heme oxy... [more]
KAG6587901.11.8e-14891.53Heme oxygenase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023531699.12.2e-14690.85heme oxygenase 1, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022145036.12.9e-14688.55heme oxygenase 1, chloroplastic [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1L1I53.4e-15393.60heme oxygenase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111500260 PE=3... [more]
A0A6J1F5E51.2e-15092.26heme oxygenase 1, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC1114410... [more]
A0A6J1CV641.4e-14688.55heme oxygenase 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111014559 P... [more]
A0A1S3B8X31.2e-14287.84heme oxygenase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103487455 PE=4 SV=... [more]
E2JEI21.9e-14086.49Chloroplast heme oxygenase-1 OS=Cucumis sativus OX=3659 GN=HO1 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT2G26670.18.4e-11269.26Plant haem oxygenase (decyclizing) family protein [more]
AT1G69720.13.8e-10477.73heme oxygenase 3 [more]
AT1G58300.11.9e-8465.79heme oxygenase 4 [more]
AT2G26670.21.8e-8256.42Plant haem oxygenase (decyclizing) family protein [more]
AT1G69720.26.1e-7073.17heme oxygenase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016951Haem oxygenase (decyclizing), plantPIRSFPIRSF030219Heme_oxygenase_decyc_plantcoord: 1..295
e-value: 1.2E-133
score: 442.8
IPR016951Haem oxygenase (decyclizing), plantPANTHERPTHR35703HEME OXYGENASE 1, CHLOROPLASTIC-RELATEDcoord: 1..295
IPR016084Haem oxygenase-like, multi-helicalGENE3D1.20.910.10coord: 82..294
e-value: 3.7E-48
score: 165.8
IPR016084Haem oxygenase-like, multi-helicalSUPERFAMILY48613Heme oxygenase-likecoord: 91..293
IPR016053Haem oxygenase-likePFAMPF01126Heme_oxygenasecoord: 125..290
e-value: 3.3E-10
score: 40.1
NoneNo IPR availablePIRSRPIRSR000343-1PIRSR000343-1coord: 125..290
e-value: 2.1E-16
score: 57.7
NoneNo IPR availablePANTHERPTHR35703:SF7BNAC06G17920D PROTEINcoord: 1..295
IPR002051Haem oxygenaseCDDcd19165HemeOcoord: 86..293
e-value: 1.57201E-56
score: 179.326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0021859.1Tan0021859.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006788 heme oxidation
biological_process GO:0015979 photosynthesis
cellular_component GO:0009507 chloroplast
molecular_function GO:0004392 heme oxygenase (decyclizing) activity