Tan0021815 (gene) Snake gourd v1

Overview
NameTan0021815
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptiontonoplast intrinsic protein 2
LocationLG05: 71839586 .. 71842148 (-)
RNA-Seq ExpressionTan0021815
SyntenyTan0021815
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATAAATCCAAAGCAATGGAGATAAGAGACTACTTCTTCGATTTCGAGTCGAAATTGAAGAGCTTACACATATCATAACGTGTCGCCTCAAAACCACGTCGTTTTAACAGGTGTCTCCTCTCTCTCTCAAACCCCATTTTTCTCCGTTTTAAATCCCACAATCCTTCACTTCTCAGCCATGCAAATTAAAAACCTTACTCTTTCTTCTAAGTCTTTTTTTATCTTTCGCGAACGAATTTAAGGAAGAAACCAAACGTGTTCTGGTTTGATTTTCTGATCGGAGACGGAGAGTTTGTGATTCGTAATTTTGAGCTGTTTTTGCCGTCGCCGCCGCTATGCCGCCGAGACGATATGCTGTTGGACGGGCCGATGAGGCCACCCACCCTGACTCCATTAGAGCCACCTTGGCCGAGTTTGTTTCCACTTTCATCTTCGTCTTTGCTGGCGAAGGCTCCGTCCTCGCTCTCGGTATGACCCGTTCCTCATTTGGTTTTTGAAAATCAAGCTTAAAAATGCCACTTTCACCCATGTATTTCTTTATTTTGTAATTTTTTGTTCGGTAACTTTTTCAAAATTAAGTTGTAAGAACTAAAAAAAATTGTTTATTTTTTAATATTTGTTTTTGTTTTTAGAAATTGGTTAAGAATTCAAGTCTTTCAAGACCATCATGCCAAAGGAATAGCGAGAAATGAACCATTTTCAAAAACAACAAAAATTATCAAATTGATACCTTAAATTTTACATAACAAAGGAAGAAATAACACTACGAGTTCGAATTTAAAACTTGCTCTAAATAAATTTAATATTCTACATTAAAATATTCAAAATAATGTATATGACTATCCTTTAATTTGTGTTTCCATCAGTATAGATTTGGGGAAGAATCAGCATCCAATTAGCATATAAACATTTTAAGAACGTGCATGCATGCATGGTCCATGGATGGATTATTAATTCTTGGTTTGCTTTGTAGATAAAATTTTCAGGCCTGCGGATTATGGAAGTTATGGTCGTGGAGGTTACGGTCACGGTTACAGTCGAAAGGGGGGCGACACGGGCCGAGGGGCATCGGATTTAGTAGTAATAGCGATAGCGCATGCATTTGCGCTGTTCTCGGCGGTAGCGGCGAGTATCAACGTCTCCGGTGGGCATGTGAATCCTGCTGTAACATTTGGTGCTCTTCTTGGAGGGAGGATCTCTCTTATCCGTGCATTCTTCTATTGGGTTGCTCAGATTTTGGGGGCCATTATCGCTTCACTTCTCTTGAGACTGGCGACTGGTGGCATGGTAAAATAAAGAAATGGAAAAACACACACACACATAATCATATAGTATTTAAAAAATTGGAAGCTTTACATTTTAATCAGTTTTGATTTTGATCTATCTGAATACCATATTAATAGTTTAAAAGAGATGAAATTTAAAATAAAGATAAAAATGATCTCCCTGTTGAATTTTAGAAACCCGGGCCCCAAATTTTTTTTTAAAAAAGAAAATTTGGAGACAATTCTAGGTGATTTACTTAAAAGCGCCTGTTTTTTTTTTCAATTTCTTTTAAATTTTAAAATAAAATATATTGGACAAGGACAACAATTTTGGGTGATTCACTTAACACCCCAAAACAAAAAAAAAAAAAAAAATGAAGAGGTATTTTTTTGGCAGAATAAAAATGTTCCAGACAATTGTTGGCGATCCTCAAATTTTAAAGATTATTTTTTTTTTCTTTCTGCAAAATATATATATATTTTTTTATAGTTCTAGATGATTCATTTAAAATACTCCCCTCAAAAGGGTGGAATTAGAAATTATGCTAATTGGAGCTATGTTGAGTGAATGGACACCTTTTACTTTTTTTTTTTATTTATTTATTTTCCTCTTTTAATAATCATATTTTATCATTAATTTCGATTGGATCATACTGAATTATCCATGCGTTTTGTTTCTTGGGCAAATTGCAGAGGCCTATGGGATTCTTCGTTTCATCAGGGATATCAGAACTGCACGGCTTTCTACTAGAGATAGTGCTTACATTCGCTTTGGTGTACACAGTTTACGCAACAGCAATAGATCCAAAGAGGGGCAGTTTGGGAACAATAGCCCCACTGGCAATCGGGTTAATAGTCGGGGCAAATATTCTGGTGGGTGGGGCCTTCGATGGGGCCTGCATGAACCCGGCCCGGGCTTTTGGGCCGTCTTTAGTGGGCTGGAGATGGGACAACCATTGGATCTACTGGATCGGCCCATTACTGGGAGGTGGCCTTGCTGCGCTTGTGTATGAGTATCTGGTCATTCCAGTGGAACCTCCTTTGCACACTCACCAGCCTTTAGCCCCTGAAGATTACTAAGCTTGCTCTTTTTTTTTTTTTTTTTATAATTTGTAAAAACAGATTCGAATGTGTGATTTGGATGTGAGACTGTTTTTATGCTCTATTTTGTTAGTTTTTTTTTTTTCTTCCTTTGAAAAGACTGGAAGCCTTTGTTGGCTGAGGGGTTGTTTGTTGTCTGGGTTGGGGTTATTTGTGGTGTTTATGTTCATGAAAGTAATAAAAAGTAGTACAGCTTA

mRNA sequence

AATAAATCCAAAGCAATGGAGATAAGAGACTACTTCTTCGATTTCGAGTCGAAATTGAAGAGCTTACACATATCATAACGTGTCGCCTCAAAACCACGTCGTTTTAACAGGTGTCTCCTCTCTCTCTCAAACCCCATTTTTCTCCGTTTTAAATCCCACAATCCTTCACTTCTCAGCCATGCAAATTAAAAACCTTACTCTTTCTTCTAAGTCTTTTTTTATCTTTCGCGAACGAATTTAAGGAAGAAACCAAACGTGTTCTGGTTTGATTTTCTGATCGGAGACGGAGAGTTTGTGATTCGTAATTTTGAGCTGTTTTTGCCGTCGCCGCCGCTATGCCGCCGAGACGATATGCTGTTGGACGGGCCGATGAGGCCACCCACCCTGACTCCATTAGAGCCACCTTGGCCGAGTTTGTTTCCACTTTCATCTTCGTCTTTGCTGGCGAAGGCTCCGTCCTCGCTCTCGATAAAATTTTCAGGCCTGCGGATTATGGAAGTTATGGTCGTGGAGGTTACGGTCACGGTTACAGTCGAAAGGGGGGCGACACGGGCCGAGGGGCATCGGATTTAGTAGTAATAGCGATAGCGCATGCATTTGCGCTGTTCTCGGCGGTAGCGGCGAGTATCAACGTCTCCGGTGGGCATGTGAATCCTGCTGTAACATTTGGTGCTCTTCTTGGAGGGAGGATCTCTCTTATCCGTGCATTCTTCTATTGGGTTGCTCAGATTTTGGGGGCCATTATCGCTTCACTTCTCTTGAGACTGGCGACTGGTGGCATGAGGCCTATGGGATTCTTCGTTTCATCAGGGATATCAGAACTGCACGGCTTTCTACTAGAGATAGTGCTTACATTCGCTTTGGTGTACACAGTTTACGCAACAGCAATAGATCCAAAGAGGGGCAGTTTGGGAACAATAGCCCCACTGGCAATCGGGTTAATAGTCGGGGCAAATATTCTGGTGGGTGGGGCCTTCGATGGGGCCTGCATGAACCCGGCCCGGGCTTTTGGGCCGTCTTTAGTGGGCTGGAGATGGGACAACCATTGGATCTACTGGATCGGCCCATTACTGGGAGGTGGCCTTGCTGCGCTTGTGTATGAGTATCTGGTCATTCCAGTGGAACCTCCTTTGCACACTCACCAGCCTTTAGCCCCTGAAGATTACTAAGCTTGCTCTTTTTTTTTTTTTTTTTATAATTTGTAAAAACAGATTCGAATGTGTGATTTGGATGTGAGACTGTTTTTATGCTCTATTTTGTTAGTTTTTTTTTTTTCTTCCTTTGAAAAGACTGGAAGCCTTTGTTGGCTGAGGGGTTGTTTGTTGTCTGGGTTGGGGTTATTTGTGGTGTTTATGTTCATGAAAGTAATAAAAAGTAGTACAGCTTA

Coding sequence (CDS)

ATGCCGCCGAGACGATATGCTGTTGGACGGGCCGATGAGGCCACCCACCCTGACTCCATTAGAGCCACCTTGGCCGAGTTTGTTTCCACTTTCATCTTCGTCTTTGCTGGCGAAGGCTCCGTCCTCGCTCTCGATAAAATTTTCAGGCCTGCGGATTATGGAAGTTATGGTCGTGGAGGTTACGGTCACGGTTACAGTCGAAAGGGGGGCGACACGGGCCGAGGGGCATCGGATTTAGTAGTAATAGCGATAGCGCATGCATTTGCGCTGTTCTCGGCGGTAGCGGCGAGTATCAACGTCTCCGGTGGGCATGTGAATCCTGCTGTAACATTTGGTGCTCTTCTTGGAGGGAGGATCTCTCTTATCCGTGCATTCTTCTATTGGGTTGCTCAGATTTTGGGGGCCATTATCGCTTCACTTCTCTTGAGACTGGCGACTGGTGGCATGAGGCCTATGGGATTCTTCGTTTCATCAGGGATATCAGAACTGCACGGCTTTCTACTAGAGATAGTGCTTACATTCGCTTTGGTGTACACAGTTTACGCAACAGCAATAGATCCAAAGAGGGGCAGTTTGGGAACAATAGCCCCACTGGCAATCGGGTTAATAGTCGGGGCAAATATTCTGGTGGGTGGGGCCTTCGATGGGGCCTGCATGAACCCGGCCCGGGCTTTTGGGCCGTCTTTAGTGGGCTGGAGATGGGACAACCATTGGATCTACTGGATCGGCCCATTACTGGGAGGTGGCCTTGCTGCGCTTGTGTATGAGTATCTGGTCATTCCAGTGGAACCTCCTTTGCACACTCACCAGCCTTTAGCCCCTGAAGATTACTAA

Protein sequence

MPPRRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRGGYGHGYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGALLGGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLTFALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Homology
BLAST of Tan0021815 vs. ExPASy Swiss-Prot
Match: O22588 (Probable aquaporin TIP3-2 OS=Arabidopsis thaliana OX=3702 GN=TIP3-2 PE=2 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.3e-106
Identity = 198/277 (71.48%), Postives = 230/277 (83.03%), Query Frame = 0

Query: 4   RRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRGGYGH 63
           R Y  GRADEATHPDSIRATLAEF+STF+FVFAGEGS+LALDK+             Y  
Sbjct: 7   RAYGFGRADEATHPDSIRATLAEFLSTFVFVFAGEGSILALDKL-------------YWD 66

Query: 64  GYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGALLGGRISLIR 123
             +  G +T  G   LV++A+AHA ALF+AV+A+INVSGGHVNPAVTF AL+GGRIS+IR
Sbjct: 67  TAAHTGTNTPGG---LVLVALAHALALFAAVSAAINVSGGHVNPAVTFAALIGGRISVIR 126

Query: 124 AFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLTFALVYTVYAT 183
           A +YWVAQ++GAI+A LLLRLAT G+RP+GF V+SG+SELHG L+EI+LTFALVY VY+T
Sbjct: 127 AIYYWVAQLIGAILACLLLRLATNGLRPVGFHVASGVSELHGLLMEIILTFALVYVVYST 186

Query: 184 AIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWIYWIG 243
           AIDPKRGS+G IAPLAIGLIVGANILVGG FDGA MNPARAFGP+LVGWRW NHWIYW+G
Sbjct: 187 AIDPKRGSIGIIAPLAIGLIVGANILVGGPFDGASMNPARAFGPALVGWRWSNHWIYWVG 246

Query: 244 PLLGGGLAALVYEYLVIPV--EPPLH-THQPLAPEDY 278
           P +GG LAAL+YEY++IP   EPP H THQPLAPEDY
Sbjct: 247 PFIGGALAALIYEYMIIPSVNEPPHHSTHQPLAPEDY 267

BLAST of Tan0021815 vs. ExPASy Swiss-Prot
Match: P26587 (Aquaporin TIP3-1 OS=Arabidopsis thaliana OX=3702 GN=TIP3-1 PE=2 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.1e-101
Identity = 186/278 (66.91%), Postives = 222/278 (79.86%), Query Frame = 0

Query: 4   RRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRGGYGH 63
           R Y  GRADEATHPDSIRATLAEF+STF+FVFA EGS+L+LDK+             Y  
Sbjct: 7   RAYGFGRADEATHPDSIRATLAEFLSTFVFVFAAEGSILSLDKL-------------YWE 66

Query: 64  GYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGALLGGRISLIR 123
             +  G +T  G   L+++A+AHAFALF+AV+A+INVSGGHVNPAVTFGAL+GGR++ IR
Sbjct: 67  HAAHAGTNTPGG---LILVALAHAFALFAAVSAAINVSGGHVNPAVTFGALVGGRVTAIR 126

Query: 124 AFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLTFALVYTVYAT 183
           A +YW+AQ+LGAI+A LLLRL T GMRP+GF ++SG+  ++G +LEI+LTF LVY VY+T
Sbjct: 127 AIYYWIAQLLGAILACLLLRLTTNGMRPVGFRLASGVGAVNGLVLEIILTFGLVYVVYST 186

Query: 184 AIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWIYWIG 243
            IDPKRGSLG IAPLAIGLIVGANILVGG F GA MNPARAFGP+LVGWRW +HWIYW+G
Sbjct: 187 LIDPKRGSLGIIAPLAIGLIVGANILVGGPFSGASMNPARAFGPALVGWRWHDHWIYWVG 246

Query: 244 PLLGGGLAALVYEYLVIPVEPPLH----THQPLAPEDY 278
           P +G  LAAL+YEY+VIP EPP H     HQPLAPEDY
Sbjct: 247 PFIGSALAALIYEYMVIPTEPPTHHAHGVHQPLAPEDY 268

BLAST of Tan0021815 vs. ExPASy Swiss-Prot
Match: P23958 (Probable aquaporin TIP-type alpha OS=Phaseolus vulgaris OX=3885 PE=1 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 1.4e-100
Identity = 184/277 (66.43%), Postives = 219/277 (79.06%), Query Frame = 0

Query: 1   MPPRRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRGG 60
           M  RRY+ GR DEATHPDS+RA+LAEF STFIFVFAGEGS LAL KI++           
Sbjct: 1   MATRRYSFGRTDEATHPDSMRASLAEFASTFIFVFAGEGSGLALVKIYQ----------- 60

Query: 61  YGHGYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGALLGGRIS 120
                     D+   A +L+ +A+AHAFALF+AV+AS++VSGGHVNPAV+FGAL+GGRIS
Sbjct: 61  ----------DSAFSAGELLALALAHAFALFAAVSASMHVSGGHVNPAVSFGALIGGRIS 120

Query: 121 LIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLTFALVYTV 180
           +IRA +YW+AQ+LG+I+A+L+LRL T  MRP GF VS G+   H F+LE+V+TF L+YTV
Sbjct: 121 VIRAVYYWIAQLLGSIVAALVLRLVTNNMRPSGFHVSPGVGVGHMFILEVVMTFGLMYTV 180

Query: 181 YATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWIY 240
           Y TAIDPKRG++  IAPLAIGLIVGANILVGG FDGACMNPA AFGPSLVGW+W  HWI+
Sbjct: 181 YGTAIDPKRGAVSYIAPLAIGLIVGANILVGGPFDGACMNPALAFGPSLVGWQWHQHWIF 240

Query: 241 WIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 278
           W+GPLLG  LAALVYEY VIP+EPP H HQPLA EDY
Sbjct: 241 WVGPLLGAALAALVYEYAVIPIEPPPHHHQPLATEDY 256

BLAST of Tan0021815 vs. ExPASy Swiss-Prot
Match: Q9ATL7 (Aquaporin TIP3-1 OS=Zea mays OX=4577 GN=TIP3-1 PE=2 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 6.1e-93
Identity = 171/278 (61.51%), Postives = 209/278 (75.18%), Query Frame = 0

Query: 2   PPRRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRGGY 61
           P RR+ VGR+++ATHPD+IRA ++EF++T IFVFA EGSVL+L K++             
Sbjct: 7   PGRRFTVGRSEDATHPDTIRAAISEFIATAIFVFAAEGSVLSLGKMY------------- 66

Query: 62  GHGYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGALLGGRISL 121
            H  S  GG        LV +A+AHA AL  AVA ++N+SGGHVNPAVTFGAL+GGR+SL
Sbjct: 67  -HDMSTAGG--------LVAVALAHALALAVAVAVAVNISGGHVNPAVTFGALVGGRVSL 126

Query: 122 IRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLTFALVYTVY 181
           +RA  YWVAQ+LGA+ A+LLLRLATGGMRP GF ++SG+ + H  LLE V+TF L+Y  Y
Sbjct: 127 VRAVLYWVAQLLGAVAATLLLRLATGGMRPPGFALASGVGDWHAVLLEAVMTFGLMYAYY 186

Query: 182 ATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWIYW 241
           AT IDPKRG +GTIAPLA+G ++GAN+L GG FDGA MNPAR FGP+LVGWRW +HW+YW
Sbjct: 187 ATVIDPKRGHVGTIAPLAVGFLLGANVLAGGPFDGAGMNPARVFGPALVGWRWRHHWVYW 246

Query: 242 IGPLLGGGLAALVYEYLVIPVEPPL--HTHQPLAPEDY 278
           +GP LG GLA LVYEYLVIP       H HQPLAPEDY
Sbjct: 247 LGPFLGAGLAGLVYEYLVIPSADAAVPHAHQPLAPEDY 262

BLAST of Tan0021815 vs. ExPASy Swiss-Prot
Match: Q9AT75 (Aquaporin TIP3-2 OS=Zea mays OX=4577 GN=TIP3-2 PE=2 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 4.4e-91
Identity = 168/275 (61.09%), Postives = 207/275 (75.27%), Query Frame = 0

Query: 4   RRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRGGYGH 63
           RR+ VGR+++ATHPD+IRA ++EF++T IFVFA EGSVL+L K++              H
Sbjct: 11  RRFTVGRSEDATHPDTIRAAISEFIATAIFVFAAEGSVLSLGKMY--------------H 70

Query: 64  GYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGALLGGRISLIR 123
            +S     T   A  LV +A+AHA  L  AVA ++NVSGGHVNPAVTFGAL+GGR+SL+R
Sbjct: 71  DHS-----TISTAGGLVAVALAHALGLAVAVAVAVNVSGGHVNPAVTFGALVGGRVSLVR 130

Query: 124 AFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLTFALVYTVYAT 183
           A  YW AQ+LGA+ A+LLLRLATGG RP GF ++SG+ + H  LLE V+TF  VY  YAT
Sbjct: 131 AVLYWAAQLLGAVAATLLLRLATGGARPPGFALASGVGDGHAVLLEAVMTFGFVYAYYAT 190

Query: 184 AIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWIYWIG 243
            +DPKRG LGTIAPLA+G ++GAN+L GG FDGA MNPAR FGP+LVGWRW +HW+YW+G
Sbjct: 191 VVDPKRGHLGTIAPLAVGFLLGANVLAGGPFDGAGMNPARVFGPALVGWRWRHHWVYWLG 250

Query: 244 PLLGGGLAALVYEYLVI-PVEPPLHTHQPLAPEDY 278
           P LG GLA LVYEYL+I P +   HTHQPLAPEDY
Sbjct: 251 PFLGAGLAGLVYEYLLIPPADAVPHTHQPLAPEDY 266

BLAST of Tan0021815 vs. NCBI nr
Match: NP_001380718.1 (aquaporin TIP3-1 [Cucumis melo])

HSP 1 Score: 529.3 bits (1362), Expect = 2.1e-146
Identity = 267/284 (94.01%), Postives = 272/284 (95.77%), Query Frame = 0

Query: 1   MPPRRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRG- 60
           MPPRRYA GRADEATHPDSIRATLAEF+STFIFVFAGEGSVLALDKIFRPADYGSYG G 
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFRPADYGSYGHGS 60

Query: 61  ------GYGHGYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGA 120
                 GYGHGY RKG DTGR ASDLVVIAIAHAFALFSAVAASIN+SGGHVNPAVTFGA
Sbjct: 61  YGRGGHGYGHGYGRKGADTGRAASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGA 120

Query: 121 LLGGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLT 180
           L+GGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSG+SELHGFLLEI+LT
Sbjct: 121 LIGGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGVSELHGFLLEIILT 180

Query: 181 FALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWR 240
           FALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWR
Sbjct: 181 FALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWR 240

Query: 241 WDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 278
           WDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 WDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 284

BLAST of Tan0021815 vs. NCBI nr
Match: XP_004137540.1 (probable aquaporin TIP3-2 [Cucumis sativus] >KGN64227.1 hypothetical protein Csa_013572 [Cucumis sativus])

HSP 1 Score: 523.5 bits (1347), Expect = 1.1e-144
Identity = 265/289 (91.70%), Postives = 271/289 (93.77%), Query Frame = 0

Query: 1   MPPRRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRG- 60
           MPPRRYA GRADEATHPDSIRATLAEF+STFIFVFAGEGSVLALDKIFRPADYGSYG G 
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFRPADYGSYGHGS 60

Query: 61  -----------GYGHGYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPA 120
                       YGHGY RKG DTGR ASDLVVIAIAHAFALFSAVAASIN+SGGHVNPA
Sbjct: 61  YGHGSYGRGGYSYGHGYGRKGTDTGRAASDLVVIAIAHAFALFSAVAASINISGGHVNPA 120

Query: 121 VTFGALLGGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLL 180
           VTFGAL+GGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSG+SELHGFLL
Sbjct: 121 VTFGALIGGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGVSELHGFLL 180

Query: 181 EIVLTFALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPS 240
           EI+LTFALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPS
Sbjct: 181 EIILTFALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPS 240

Query: 241 LVGWRWDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 278
           LVGWRWDNHWIYWIGPL+GGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 LVGWRWDNHWIYWIGPLIGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 289

BLAST of Tan0021815 vs. NCBI nr
Match: XP_022923672.1 (probable aquaporin TIP3-2 [Cucurbita moschata])

HSP 1 Score: 522.7 bits (1345), Expect = 1.9e-144
Identity = 264/279 (94.62%), Postives = 269/279 (96.42%), Query Frame = 0

Query: 1   MPPRRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRGG 60
           MPPRRYA GRADEATHPDSIRATLAEF+STFIFVFAGEGSVLALDKIF PADYGSYG GG
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60

Query: 61  Y--GHGYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGALLGGR 120
           Y  GHGY R+  DTGRGASDLVVIAIAHAFALFSAVAASIN+SGGHVNPAVTFGALLGGR
Sbjct: 61  YSRGHGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120

Query: 121 ISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLTFALVY 180
           ISLIR  FYWVAQILGAI+ASLLLRLATGGMRPMGFFVSSGISE+HGFLLEIVLTFALVY
Sbjct: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180

Query: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240
           TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW
Sbjct: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240

Query: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 278
           IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 279

BLAST of Tan0021815 vs. NCBI nr
Match: XP_023519163.1 (probable aquaporin TIP3-2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 522.7 bits (1345), Expect = 1.9e-144
Identity = 264/279 (94.62%), Postives = 269/279 (96.42%), Query Frame = 0

Query: 1   MPPRRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRGG 60
           MPPRRYA GRADEATHPDSIRATLAEF+STFIFVFAGEGSVLALDKIF PADYGSYG GG
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60

Query: 61  Y--GHGYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGALLGGR 120
           Y  GHGY R+  DTGRGASDLVVIAIAHAFALFSAVAASIN+SGGHVNPAVTFGALLGGR
Sbjct: 61  YSHGHGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120

Query: 121 ISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLTFALVY 180
           ISLIR  FYWVAQILGAI+ASLLLRLATGGMRPMGFFVSSGISE+HGFLLEIVLTFALVY
Sbjct: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180

Query: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240
           TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW
Sbjct: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240

Query: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 278
           IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 279

BLAST of Tan0021815 vs. NCBI nr
Match: KAG7019875.1 (putative aquaporin TIP3-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 522.7 bits (1345), Expect = 1.9e-144
Identity = 264/279 (94.62%), Postives = 269/279 (96.42%), Query Frame = 0

Query: 1   MPPRRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRGG 60
           MPPRRYA GRADEATHPDSIRATLAEF+STFIFVFAGEGSVLALDKIF PADYGSYG GG
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60

Query: 61  Y--GHGYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGALLGGR 120
           Y  GHGY R+  DTGRGASDLVVIAIAHAFALFSAVAASIN+SGGHVNPAVTFGALLGGR
Sbjct: 61  YSHGHGYGRRKTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120

Query: 121 ISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLTFALVY 180
           ISLIR  FYWVAQILGAI+ASLLLRLATGGMRPMGFFVSSGISE+HGFLLEIVLTFALVY
Sbjct: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180

Query: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240
           TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW
Sbjct: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240

Query: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 278
           IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 279

BLAST of Tan0021815 vs. ExPASy TrEMBL
Match: A0A1S3AU56 (probable aquaporin TIP3-2 OS=Cucumis melo OX=3656 GN=LOC103482730 PE=3 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.0e-146
Identity = 267/284 (94.01%), Postives = 272/284 (95.77%), Query Frame = 0

Query: 1   MPPRRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRG- 60
           MPPRRYA GRADEATHPDSIRATLAEF+STFIFVFAGEGSVLALDKIFRPADYGSYG G 
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFRPADYGSYGHGS 60

Query: 61  ------GYGHGYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGA 120
                 GYGHGY RKG DTGR ASDLVVIAIAHAFALFSAVAASIN+SGGHVNPAVTFGA
Sbjct: 61  YGRGGHGYGHGYGRKGADTGRAASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGA 120

Query: 121 LLGGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLT 180
           L+GGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSG+SELHGFLLEI+LT
Sbjct: 121 LIGGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGVSELHGFLLEIILT 180

Query: 181 FALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWR 240
           FALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWR
Sbjct: 181 FALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWR 240

Query: 241 WDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 278
           WDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 WDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 284

BLAST of Tan0021815 vs. ExPASy TrEMBL
Match: A0A0A0LTX0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043290 PE=3 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 5.5e-145
Identity = 265/289 (91.70%), Postives = 271/289 (93.77%), Query Frame = 0

Query: 1   MPPRRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRG- 60
           MPPRRYA GRADEATHPDSIRATLAEF+STFIFVFAGEGSVLALDKIFRPADYGSYG G 
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFRPADYGSYGHGS 60

Query: 61  -----------GYGHGYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPA 120
                       YGHGY RKG DTGR ASDLVVIAIAHAFALFSAVAASIN+SGGHVNPA
Sbjct: 61  YGHGSYGRGGYSYGHGYGRKGTDTGRAASDLVVIAIAHAFALFSAVAASINISGGHVNPA 120

Query: 121 VTFGALLGGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLL 180
           VTFGAL+GGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSG+SELHGFLL
Sbjct: 121 VTFGALIGGRISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGVSELHGFLL 180

Query: 181 EIVLTFALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPS 240
           EI+LTFALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPS
Sbjct: 181 EIILTFALVYTVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPS 240

Query: 241 LVGWRWDNHWIYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 278
           LVGWRWDNHWIYWIGPL+GGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 LVGWRWDNHWIYWIGPLIGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 289

BLAST of Tan0021815 vs. ExPASy TrEMBL
Match: A0A6J1E6R9 (probable aquaporin TIP3-2 OS=Cucurbita moschata OX=3662 GN=LOC111431313 PE=3 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 9.3e-145
Identity = 264/279 (94.62%), Postives = 269/279 (96.42%), Query Frame = 0

Query: 1   MPPRRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRGG 60
           MPPRRYA GRADEATHPDSIRATLAEF+STFIFVFAGEGSVLALDKIF PADYGSYG GG
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60

Query: 61  Y--GHGYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGALLGGR 120
           Y  GHGY R+  DTGRGASDLVVIAIAHAFALFSAVAASIN+SGGHVNPAVTFGALLGGR
Sbjct: 61  YSRGHGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120

Query: 121 ISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLTFALVY 180
           ISLIR  FYWVAQILGAI+ASLLLRLATGGMRPMGFFVSSGISE+HGFLLEIVLTFALVY
Sbjct: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180

Query: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240
           TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW
Sbjct: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240

Query: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 278
           IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 279

BLAST of Tan0021815 vs. ExPASy TrEMBL
Match: A0A6J1KM69 (probable aquaporin TIP3-2 OS=Cucurbita maxima OX=3661 GN=LOC111495835 PE=3 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 4.6e-144
Identity = 263/279 (94.27%), Postives = 268/279 (96.06%), Query Frame = 0

Query: 1   MPPRRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRGG 60
           MPPRRYA GRADEATHPDSIRATLAEF+STFIFVFAGEGSVLALDKIF PADYGSYG GG
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60

Query: 61  YGH--GYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGALLGGR 120
           Y H  GY R+  DTGRGASDLVVIAIAHAFALFSAVAASIN+SGGHVNPAVTFGALLGGR
Sbjct: 61  YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120

Query: 121 ISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLTFALVY 180
           ISLIR  FYWVAQILGAI+ASLLLRLATGGMRPMGFFVSSGISE+HGFLLEIVLTFALVY
Sbjct: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180

Query: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240
           TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW
Sbjct: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240

Query: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 278
           IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 279

BLAST of Tan0021815 vs. ExPASy TrEMBL
Match: Q39646 (MP23 OS=Cucurbita hybrid cultivar OX=170850 PE=2 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 4.6e-144
Identity = 263/279 (94.27%), Postives = 268/279 (96.06%), Query Frame = 0

Query: 1   MPPRRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRGG 60
           MPPRRYA GRADEATHPDSIRATLAEF+STFIFVFAGEGSVLALDKIF PADYGSYG GG
Sbjct: 1   MPPRRYAFGRADEATHPDSIRATLAEFISTFIFVFAGEGSVLALDKIFSPADYGSYGHGG 60

Query: 61  YGH--GYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGALLGGR 120
           Y H  GY R+  DTGRGASDLVVIAIAHAFALFSAVAASIN+SGGHVNPAVTFGALLGGR
Sbjct: 61  YSHGDGYGRRRTDTGRGASDLVVIAIAHAFALFSAVAASINISGGHVNPAVTFGALLGGR 120

Query: 121 ISLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLTFALVY 180
           ISLIR  FYWVAQILGAI+ASLLLRLATGGMRPMGFFVSSGISE+HGFLLEIVLTFALVY
Sbjct: 121 ISLIRGIFYWVAQILGAIVASLLLRLATGGMRPMGFFVSSGISEMHGFLLEIVLTFALVY 180

Query: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240
           TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW
Sbjct: 181 TVYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHW 240

Query: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 278
           IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY
Sbjct: 241 IYWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 279

BLAST of Tan0021815 vs. TAIR 10
Match: AT1G17810.1 (beta-tonoplast intrinsic protein )

HSP 1 Score: 387.5 bits (994), Expect = 9.0e-108
Identity = 198/277 (71.48%), Postives = 230/277 (83.03%), Query Frame = 0

Query: 4   RRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRGGYGH 63
           R Y  GRADEATHPDSIRATLAEF+STF+FVFAGEGS+LALDK+             Y  
Sbjct: 7   RAYGFGRADEATHPDSIRATLAEFLSTFVFVFAGEGSILALDKL-------------YWD 66

Query: 64  GYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGALLGGRISLIR 123
             +  G +T  G   LV++A+AHA ALF+AV+A+INVSGGHVNPAVTF AL+GGRIS+IR
Sbjct: 67  TAAHTGTNTPGG---LVLVALAHALALFAAVSAAINVSGGHVNPAVTFAALIGGRISVIR 126

Query: 124 AFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLTFALVYTVYAT 183
           A +YWVAQ++GAI+A LLLRLAT G+RP+GF V+SG+SELHG L+EI+LTFALVY VY+T
Sbjct: 127 AIYYWVAQLIGAILACLLLRLATNGLRPVGFHVASGVSELHGLLMEIILTFALVYVVYST 186

Query: 184 AIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWIYWIG 243
           AIDPKRGS+G IAPLAIGLIVGANILVGG FDGA MNPARAFGP+LVGWRW NHWIYW+G
Sbjct: 187 AIDPKRGSIGIIAPLAIGLIVGANILVGGPFDGASMNPARAFGPALVGWRWSNHWIYWVG 246

Query: 244 PLLGGGLAALVYEYLVIPV--EPPLH-THQPLAPEDY 278
           P +GG LAAL+YEY++IP   EPP H THQPLAPEDY
Sbjct: 247 PFIGGALAALIYEYMIIPSVNEPPHHSTHQPLAPEDY 267

BLAST of Tan0021815 vs. TAIR 10
Match: AT1G73190.1 (Aquaporin-like superfamily protein )

HSP 1 Score: 370.2 bits (949), Expect = 1.5e-102
Identity = 186/278 (66.91%), Postives = 222/278 (79.86%), Query Frame = 0

Query: 4   RRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRGGYGH 63
           R Y  GRADEATHPDSIRATLAEF+STF+FVFA EGS+L+LDK+             Y  
Sbjct: 7   RAYGFGRADEATHPDSIRATLAEFLSTFVFVFAAEGSILSLDKL-------------YWE 66

Query: 64  GYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGALLGGRISLIR 123
             +  G +T  G   L+++A+AHAFALF+AV+A+INVSGGHVNPAVTFGAL+GGR++ IR
Sbjct: 67  HAAHAGTNTPGG---LILVALAHAFALFAAVSAAINVSGGHVNPAVTFGALVGGRVTAIR 126

Query: 124 AFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLTFALVYTVYAT 183
           A +YW+AQ+LGAI+A LLLRL T GMRP+GF ++SG+  ++G +LEI+LTF LVY VY+T
Sbjct: 127 AIYYWIAQLLGAILACLLLRLTTNGMRPVGFRLASGVGAVNGLVLEIILTFGLVYVVYST 186

Query: 184 AIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWIYWIG 243
            IDPKRGSLG IAPLAIGLIVGANILVGG F GA MNPARAFGP+LVGWRW +HWIYW+G
Sbjct: 187 LIDPKRGSLGIIAPLAIGLIVGANILVGGPFSGASMNPARAFGPALVGWRWHDHWIYWVG 246

Query: 244 PLLGGGLAALVYEYLVIPVEPPLH----THQPLAPEDY 278
           P +G  LAAL+YEY+VIP EPP H     HQPLAPEDY
Sbjct: 247 PFIGSALAALIYEYMVIPTEPPTHHAHGVHQPLAPEDY 268

BLAST of Tan0021815 vs. TAIR 10
Match: AT2G36830.1 (gamma tonoplast intrinsic protein )

HSP 1 Score: 298.5 bits (763), Expect = 5.5e-81
Identity = 153/277 (55.23%), Postives = 190/277 (68.59%), Query Frame = 0

Query: 1   MPPRRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRGG 60
           MP R  A+GR DEAT PD+++A LAEF+ST IFV AG GS +A +K+             
Sbjct: 1   MPIRNIAIGRPDEATRPDALKAALAEFISTLIFVVAGSGSGMAFNKL------------- 60

Query: 61  YGHGYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGALLGGRIS 120
                +  G  T  G   LV  A+AHAF LF AV+   N+SGGHVNPAVTFGA +GG I+
Sbjct: 61  -----TENGATTPSG---LVAAAVAHAFGLFVAVSVGANISGGHVNPAVTFGAFIGGNIT 120

Query: 121 LIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLTFALVYTV 180
           L+R   YW+AQ+LG+++A L+L+ ATGG+    F +S+G+  L+ F+ EIV+TF LVYTV
Sbjct: 121 LLRGILYWIAQLLGSVVACLILKFATGGLAVPAFGLSAGVGVLNAFVFEIVMTFGLVYTV 180

Query: 181 YATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWIY 240
           YATAIDPK GSLGTIAP+AIG IVGANIL GGAF GA MNPA AFGP++V W W NHW+Y
Sbjct: 181 YATAIDPKNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWTWTNHWVY 240

Query: 241 WIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 278
           W GPL+GGG+A L+YE   I       TH+ L   DY
Sbjct: 241 WAGPLVGGGIAGLIYEVFFINT-----THEQLPTTDY 251

BLAST of Tan0021815 vs. TAIR 10
Match: AT4G01470.1 (tonoplast intrinsic protein 1;3 )

HSP 1 Score: 284.3 bits (726), Expect = 1.1e-76
Identity = 146/277 (52.71%), Postives = 188/277 (67.87%), Query Frame = 0

Query: 1   MPPRRYAVGRADEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRGG 60
           MP  R A+G   EA+ PD+IRA  AEF S  IFVFAG+GS                    
Sbjct: 1   MPINRIAIGTPGEASRPDAIRAAFAEFFSMVIFVFAGQGS-------------------- 60

Query: 61  YGHGYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGALLGGRIS 120
            G  Y +  GD     + LV  +++HAFALF AV+   NVSGGHVNPAVTFGA +GG I+
Sbjct: 61  -GMAYGKLTGDGPATPAGLVAASLSHAFALFVAVSVGANVSGGHVNPAVTFGAFIGGNIT 120

Query: 121 LIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLTFALVYTV 180
           L+RA  YW+AQ+LGA++A LLL+++TGGM    F +S G++  +  + EIV+TF LVYTV
Sbjct: 121 LLRAILYWIAQLLGAVVACLLLKVSTGGMETAAFSLSYGVTPWNAVVFEIVMTFGLVYTV 180

Query: 181 YATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWIY 240
           YATA+DPK+G +G IAPLAIGLIVGANILVGGAFDGA MNPA +FGP++V W W NHW+Y
Sbjct: 181 YATAVDPKKGDIGIIAPLAIGLIVGANILVGGAFDGASMNPAVSFGPAVVSWIWTNHWVY 240

Query: 241 WIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 278
           W+GP +G  +AA+VY+ + I      + H+PL   D+
Sbjct: 241 WVGPFIGAAIAAIVYDTIFI----GSNGHEPLPSNDF 252

BLAST of Tan0021815 vs. TAIR 10
Match: AT3G26520.1 (tonoplast intrinsic protein 2 )

HSP 1 Score: 283.9 bits (725), Expect = 1.4e-76
Identity = 149/278 (53.60%), Postives = 186/278 (66.91%), Query Frame = 0

Query: 1   MPPRRYAVGRA-DEATHPDSIRATLAEFVSTFIFVFAGEGSVLALDKIFRPADYGSYGRG 60
           MP R  A+G   +E  HP+++RA LAEF+ST IFVFAG GS +A +KI            
Sbjct: 1   MPTRNIAIGGVQEEVYHPNALRAALAEFISTLIFVFAGSGSGIAFNKI------------ 60

Query: 61  GYGHGYSRKGGDTGRGASDLVVIAIAHAFALFSAVAASINVSGGHVNPAVTFGALLGGRI 120
                 +  G  T  G   LV  A+AHAF LF AV+   N+SGGHVNPAVTFG LLGG I
Sbjct: 61  ------TDNGATTPSG---LVAAALAHAFGLFVAVSVGANISGGHVNPAVTFGVLLGGNI 120

Query: 121 SLIRAFFYWVAQILGAIIASLLLRLATGGMRPMGFFVSSGISELHGFLLEIVLTFALVYT 180
           +L+R   YW+AQ+LG++ A  LL  ATGG     F +S+G+  L+  + EIV+TF LVYT
Sbjct: 121 TLLRGILYWIAQLLGSVAACFLLSFATGGEPIPAFGLSAGVGSLNALVFEIVMTFGLVYT 180

Query: 181 VYATAIDPKRGSLGTIAPLAIGLIVGANILVGGAFDGACMNPARAFGPSLVGWRWDNHWI 240
           VYATA+DPK GSLGTIAP+AIG IVGANIL GGAF GA MNPA AFGP++V W W NHW+
Sbjct: 181 VYATAVDPKNGSLGTIAPIAIGFIVGANILAGGAFSGASMNPAVAFGPAVVSWTWTNHWV 240

Query: 241 YWIGPLLGGGLAALVYEYLVIPVEPPLHTHQPLAPEDY 278
           YW GPL+GGGLA ++Y+++ I      + H+ L   DY
Sbjct: 241 YWAGPLIGGGLAGIIYDFVFIDE----NAHEQLPTTDY 253

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O225881.3e-10671.48Probable aquaporin TIP3-2 OS=Arabidopsis thaliana OX=3702 GN=TIP3-2 PE=2 SV=1[more]
P265872.1e-10166.91Aquaporin TIP3-1 OS=Arabidopsis thaliana OX=3702 GN=TIP3-1 PE=2 SV=1[more]
P239581.4e-10066.43Probable aquaporin TIP-type alpha OS=Phaseolus vulgaris OX=3885 PE=1 SV=1[more]
Q9ATL76.1e-9361.51Aquaporin TIP3-1 OS=Zea mays OX=4577 GN=TIP3-1 PE=2 SV=1[more]
Q9AT754.4e-9161.09Aquaporin TIP3-2 OS=Zea mays OX=4577 GN=TIP3-2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
NP_001380718.12.1e-14694.01aquaporin TIP3-1 [Cucumis melo][more]
XP_004137540.11.1e-14491.70probable aquaporin TIP3-2 [Cucumis sativus] >KGN64227.1 hypothetical protein Csa... [more]
XP_022923672.11.9e-14494.62probable aquaporin TIP3-2 [Cucurbita moschata][more]
XP_023519163.11.9e-14494.62probable aquaporin TIP3-2 [Cucurbita pepo subsp. pepo][more]
KAG7019875.11.9e-14494.62putative aquaporin TIP3-2, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A1S3AU561.0e-14694.01probable aquaporin TIP3-2 OS=Cucumis melo OX=3656 GN=LOC103482730 PE=3 SV=1[more]
A0A0A0LTX05.5e-14591.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G043290 PE=3 SV=1[more]
A0A6J1E6R99.3e-14594.62probable aquaporin TIP3-2 OS=Cucurbita moschata OX=3662 GN=LOC111431313 PE=3 SV=... [more]
A0A6J1KM694.6e-14494.27probable aquaporin TIP3-2 OS=Cucurbita maxima OX=3661 GN=LOC111495835 PE=3 SV=1[more]
Q396464.6e-14494.27MP23 OS=Cucurbita hybrid cultivar OX=170850 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G17810.19.0e-10871.48beta-tonoplast intrinsic protein [more]
AT1G73190.11.5e-10266.91Aquaporin-like superfamily protein [more]
AT2G36830.15.5e-8155.23gamma tonoplast intrinsic protein [more]
AT4G01470.11.1e-7652.71tonoplast intrinsic protein 1;3 [more]
AT3G26520.11.4e-7653.60tonoplast intrinsic protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000425Major intrinsic proteinPRINTSPR00783MINTRINSICPcoord: 21..40
score: 43.9
coord: 238..258
score: 44.9
coord: 123..142
score: 43.62
coord: 168..186
score: 41.95
coord: 201..223
score: 42.55
coord: 86..110
score: 45.71
IPR000425Major intrinsic proteinTIGRFAMTIGR00861TIGR00861coord: 25..255
e-value: 2.7E-57
score: 192.1
IPR000425Major intrinsic proteinPFAMPF00230MIPcoord: 14..255
e-value: 9.2E-74
score: 248.0
IPR000425Major intrinsic proteinCDDcd00333MIPcoord: 21..258
e-value: 1.77101E-69
score: 212.499
IPR023271Aquaporin-likeGENE3D1.20.1080.10Glycerol uptake facilitator protein.coord: 5..263
e-value: 1.4E-76
score: 258.8
IPR023271Aquaporin-likeSUPERFAMILY81338Aquaporin-likecoord: 13..268
IPR034294Aquaporin transporterPANTHERPTHR45665AQUAPORIN-8coord: 3..49
IPR034294Aquaporin transporterPANTHERPTHR45665AQUAPORIN-8coord: 70..277
NoneNo IPR availablePANTHERPTHR45665:SF39SUBFAMILY NOT NAMEDcoord: 70..277
coord: 3..49
IPR022357Major intrinsic protein, conserved sitePROSITEPS00221MIPcoord: 104..112

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0021815.1Tan0021815.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
biological_process GO:0006833 water transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0015250 water channel activity
molecular_function GO:0015267 channel activity