Tan0021690 (gene) Snake gourd v1

Overview


Name	Tan0021690
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Steroid 5-alpha-reductase DET2
Location	LG08: 72818435 .. 72819419 (-)
RNA-Seq Expression	Tan0021690
Synteny	Tan0021690

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCTCATTTGTGCTTTCTGCAACCATAGCCTTGAGCACTACAGGCTTCGATCTAAGTGAAAGTTTCTCTGAGAATGTAGATTCCGAGTTTCTACCACAAAATTGAAGAGTGAAGACAATTCAGCCACAAATTTCCCCATTTTCAAACTCAATTTGAATCTTCGAATCCTTCACTTATGGGTTCGGATCAGGCTCTGTTCCACAACAGCCTCCTCGCCCTGTTCCTCATCGCACCACCGACCGTCGTCGGCCTGAGGTTCCTCCAGGCTCCGTACGGGAAGCACAACCGGCCGGGTTGGGGTCCAACCATGTCCGCTCCATTGGCCTGGTTTCTAATGGAAAGCCCCACTCTGTGGCTGACCCTCCTCCTCTTCCCCTTCGGCGATCACTCCTCCGATCCCAAGCCTCTCTTTCTCATCTCCCCCTTCCTCCTCCACTACCTCAACCGCACCTTCCTCTACCCGATTCGCCTTCTCTGGACTGGGCCTCGACGGAATACCGCCGCCGGTTTCCCGGTGGTTGTGGCACTCATGGCATTCGGGTTCAATTTGCTCAATTCTTACCTGCAAACCAGATCGGTGTCTCACTACGTGGACTACGACGGCGACGGGTGGTTTTGGTGGCGCTTTTTTGGTGGAGGGGTGGTTTTCCTGTCGGGTATGGCGGTGAATATTTGGTCGGATAGTGTTCTGGTCGGACTGAAGAGCGCCGGCGGCGGGTATAAGCTGCCGACGGGAGGGTGGTTTGAGTTTGTGAGTTGCCCTAATTATTTGGGGGAGATTGTGGAGTGGCTGGGCTGGGCTGTGATGTGCGGGTCATGGGCCGGGCTGAGTTTTTTTCTTTACACGTGTGCCAATTTAGTGCCTAGGGCACGTGCGAACCACACGTGGTATTTGAATAAGTTTGGGGAGGATTATCCAAAATGTAGGAAGGCTGTTATTCCCTTCTTGTATTGAGAAACTGCATCAAAATGTGAATTCAAATAAT

mRNA sequence

CCTCATTTGTGCTTTCTGCAACCATAGCCTTGAGCACTACAGGCTTCGATCTAAGTGAAAGTTTCTCTGAGAATGTAGATTCCGAGTTTCTACCACAAAATTGAAGAGTGAAGACAATTCAGCCACAAATTTCCCCATTTTCAAACTCAATTTGAATCTTCGAATCCTTCACTTATGGGTTCGGATCAGGCTCTGTTCCACAACAGCCTCCTCGCCCTGTTCCTCATCGCACCACCGACCGTCGTCGGCCTGAGGTTCCTCCAGGCTCCGTACGGGAAGCACAACCGGCCGGGTTGGGGTCCAACCATGTCCGCTCCATTGGCCTGGTTTCTAATGGAAAGCCCCACTCTGTGGCTGACCCTCCTCCTCTTCCCCTTCGGCGATCACTCCTCCGATCCCAAGCCTCTCTTTCTCATCTCCCCCTTCCTCCTCCACTACCTCAACCGCACCTTCCTCTACCCGATTCGCCTTCTCTGGACTGGGCCTCGACGGAATACCGCCGCCGGTTTCCCGGTGGTTGTGGCACTCATGGCATTCGGGTTCAATTTGCTCAATTCTTACCTGCAAACCAGATCGGTGTCTCACTACGTGGACTACGACGGCGACGGGTGGTTTTGGTGGCGCTTTTTTGGTGGAGGGGTGGTTTTCCTGTCGGGTATGGCGGTGAATATTTGGTCGGATAGTGTTCTGGTCGGACTGAAGAGCGCCGGCGGCGGGTATAAGCTGCCGACGGGAGGGTGGTTTGAGTTTGTGAGTTGCCCTAATTATTTGGGGGAGATTGTGGAGTGGCTGGGCTGGGCTGTGATGTGCGGGTCATGGGCCGGGCTGAGTTTTTTTCTTTACACGTGTGCCAATTTAGTGCCTAGGGCACGTGCGAACCACACGTGGTATTTGAATAAGTTTGGGGAGGATTATCCAAAATGTAGGAAGGCTGTTATTCCCTTCTTGTATTGAGAAACTGCATCAAAATGTGAATTCAAATAAT

Coding sequence (CDS)

ATGGGTTCGGATCAGGCTCTGTTCCACAACAGCCTCCTCGCCCTGTTCCTCATCGCACCACCGACCGTCGTCGGCCTGAGGTTCCTCCAGGCTCCGTACGGGAAGCACAACCGGCCGGGTTGGGGTCCAACCATGTCCGCTCCATTGGCCTGGTTTCTAATGGAAAGCCCCACTCTGTGGCTGACCCTCCTCCTCTTCCCCTTCGGCGATCACTCCTCCGATCCCAAGCCTCTCTTTCTCATCTCCCCCTTCCTCCTCCACTACCTCAACCGCACCTTCCTCTACCCGATTCGCCTTCTCTGGACTGGGCCTCGACGGAATACCGCCGCCGGTTTCCCGGTGGTTGTGGCACTCATGGCATTCGGGTTCAATTTGCTCAATTCTTACCTGCAAACCAGATCGGTGTCTCACTACGTGGACTACGACGGCGACGGGTGGTTTTGGTGGCGCTTTTTTGGTGGAGGGGTGGTTTTCCTGTCGGGTATGGCGGTGAATATTTGGTCGGATAGTGTTCTGGTCGGACTGAAGAGCGCCGGCGGCGGGTATAAGCTGCCGACGGGAGGGTGGTTTGAGTTTGTGAGTTGCCCTAATTATTTGGGGGAGATTGTGGAGTGGCTGGGCTGGGCTGTGATGTGCGGGTCATGGGCCGGGCTGAGTTTTTTTCTTTACACGTGTGCCAATTTAGTGCCTAGGGCACGTGCGAACCACACGTGGTATTTGAATAAGTTTGGGGAGGATTATCCAAAATGTAGGAAGGCTGTTATTCCCTTCTTGTATTGA

Protein sequence

MGSDQALFHNSLLALFLIAPPTVVGLRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLWLTLLLFPFGDHSSDPKPLFLISPFLLHYLNRTFLYPIRLLWTGPRRNTAAGFPVVVALMAFGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFFGGGVVFLSGMAVNIWSDSVLVGLKSAGGGYKLPTGGWFEFVSCPNYLGEIVEWLGWAVMCGSWAGLSFFLYTCANLVPRARANHTWYLNKFGEDYPKCRKAVIPFLY

Homology

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BLAST of Tan0021690 vs. ExPASy Swiss-Prot
Match: Q2QDF6 (Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 1.8e-99
Identity = 177/263 (67.30%), Postives = 195/263 (74.14%), Query Frame = 0


Query: 1   MGSDQALFHNSLLALFLIAPPTVVGLRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 60
           M SDQ LFH  LL L++IA PT + L FLQAPYGKHNRPGWGPT+S PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHYCLLTLYIIALPTWISLYFLQAPYGKHNRPGWGPTISPPLAWFLMESPTLW 60

Query: 61  LTLLLFPFGDHSSDPKPLFLISPFLLHYLNRTFLYPIRLLWTGPRRNT--AAGFPVVVAL 120
           LT  LFP G H  +PK   LISPFL HY NRT LYP+RL      RNT    GFPV VA 
Sbjct: 61  LTFFLFPSGQHFYNPKSFLLISPFLFHYFNRTVLYPLRL-----ARNTTQTRGFPVSVAF 120

Query: 121 MAFGFNLLNSYLQTRSVSHYV-DYDGDGWFWWRFFGGGVVFLSGMAVNIWSDSVLVGLKS 180
           MAFGFNLLN YLQ R VSHY  DY+ +  FWWRF  G ++F+ GM VN+ +D VLVGLK 
Sbjct: 121 MAFGFNLLNGYLQARWVSHYKDDYENEELFWWRFLAGLLIFVVGMWVNVRADKVLVGLKK 180

Query: 181 AG-GGYKLPTGGWFEFVSCPNYLGEIVEWLGWAVMCGSWAGLSFFLYTCANLVPRARANH 240
            G GGYK+P GG FE VSCPNY GEI+EW GWAVM  SW G  FFLYTCANL+PRARA  
Sbjct: 181 QGDGGYKIPRGGLFELVSCPNYFGEIMEWFGWAVMTWSWVGFGFFLYTCANLMPRARATR 240

Query: 241 TWYLNKFGEDYPKCRKAVIPFLY 260
            WYL KF +DYPK RKAVIPF+Y
Sbjct: 241 LWYLEKFKDDYPKDRKAVIPFIY 258

[Close]

BLAST of Tan0021690 vs. ExPASy Swiss-Prot
Match: Q38944 (Steroid 5-alpha-reductase DET2 OS=Arabidopsis thaliana OX=3702 GN=DET2 PE=1 SV=2)

HSP 1 Score: 349.7 bits (896), Expect = 2.7e-95
Identity = 163/260 (62.69%), Postives = 193/260 (74.23%), Query Frame = 0


Query: 3   SDQALFHNSLLALFLIAPPTVVGLRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLWLT 62
           +D+  F   LL L    PPT V L+FLQAPYGKHNR GWGPT+S P+AWF+MESPTLWLT
Sbjct: 5   ADKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLT 64

Query: 63  LLLFPFGDHSSDPKPLFLISPFLLHYLNRTFLYPIRLLWTG-PRRNTAAGFPVVVALMAF 122
           LLLFPFG H+ +PK L L SP+L+HY +RT +YP+RL  +  P      GFP+ +A +AF
Sbjct: 65  LLLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKN--GFPITIAALAF 124

Query: 123 GFNLLNSYLQTRSVSHYV-DYDGDGWFWWRFFGGGVVFLSGMAVNIWSDSVLVGLKSAG- 182
            FNLLN Y+Q R VSHY  DY+   WFWWRF  G VVF++GM +NI SD  LV LK    
Sbjct: 125 TFNLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENR 184

Query: 183 GGYKLPTGGWFEFVSCPNYLGEIVEWLGWAVMCGSWAGLSFFLYTCANLVPRARANHTWY 242
           GGY +P GGWFE VSCPNY GE +EWLGWAVM  SWAG+ FFLYTC+NL PRARA+H WY
Sbjct: 185 GGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHKWY 244

Query: 243 LNKFGEDYPKCRKAVIPFLY 260
           + KF E+YPK RKAVIPF+Y
Sbjct: 245 IAKFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of Tan0021690 vs. ExPASy Swiss-Prot
Match: Q5K2N1 (Steroid 5-alpha-reductase DET2 OS=Solanum lycopersicum OX=4081 GN=DET2 PE=1 SV=1)

HSP 1 Score: 317.4 bits (812), Expect = 1.5e-85
Identity = 151/258 (58.53%), Postives = 190/258 (73.64%), Query Frame = 0


Query: 3   SDQALFHNSLLALFLIAPPTVVGLRFLQAPYGKH-NRPGWGPTMSAPLAWFLMESPTLWL 62
           SD+ LF+  +  + ++A PT +  +F  +PYGKH      G T+S P+AW  MESPTLWL
Sbjct: 4   SDENLFNFIVFFILVMAFPTFILCQFFTSPYGKHYTSADSGTTISPPIAWAFMESPTLWL 63

Query: 63  TLLLFPFGDHSSDPKPLFLISPFLLHYLNRTFLYPIRLLWTGPRRNTAAGFPVVVALMAF 122
           T+++F  G + ++P    LISP+L HY NRT +YP+RL      RNT   FP+ +A+ AF
Sbjct: 64  TIIVFRLGKNYTNPLAFLLISPYLFHYTNRTIIYPLRL----RSRNTKNNFPLNIAVTAF 123

Query: 123 GFNLLNSYLQTRSVSHYVDYDGDGWFWWRFFGGGVVFLSGMAVNIWSDSVLVGLKSAGGG 182
            FNLLN+Y+Q+R VSHY +Y  D WFW RF  G V+F SGM +NIW+D VL+GLKS GGG
Sbjct: 124 IFNLLNAYIQSRWVSHYANYQEDDWFWVRFGIGLVIFGSGMLLNIWADGVLLGLKSQGGG 183

Query: 183 YKLPTGGWFEFVSCPNYLGEIVEWLGWAVMCGSWAGLSFFLYTCANLVPRARANHTWYLN 242
           YK+P GG F++VS PNYLGEI+EWLGWA+M  SWAGL+FF+YTCANLVPRA +NH WYL 
Sbjct: 184 YKIPRGGLFDYVSSPNYLGEIMEWLGWALMTWSWAGLAFFVYTCANLVPRAVSNHKWYLQ 243

Query: 243 KFGEDYPKCRKAVIPFLY 260
           KFGEDYPK RKAV PFLY
Sbjct: 244 KFGEDYPKNRKAVFPFLY 257

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BLAST of Tan0021690 vs. ExPASy Swiss-Prot
Match: P24008 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 PE=1 SV=2)

HSP 1 Score: 154.1 bits (388), Expect = 2.2e-36
Identity = 93/241 (38.59%), Postives = 139/241 (57.68%), Query Frame = 0


Query: 22  TVVGLRFLQAPYGKHNRPGW-GPTMSAPLAWFLMESPTL-W-LTLLLFPFGDHSSDPKPL 81
           ++VGLR + +PYG+++ P W G  + A  AWF+ E P++ W L   + P      +    
Sbjct: 27  SIVGLRSVGSPYGRYS-PQWPGIRVPARPAWFIQELPSMAWPLYEYIRPAAARLGNLPNR 86

Query: 82  FLISPFLLHYLNRTFLYPIRLLWTGPRRNTAAGFPVVVALMAFGFNLLNSYLQTRSVSHY 141
            L++ FL+HY+ RT ++P+ +    P         +V  ++AF F   N Y+Q+R +S +
Sbjct: 87  VLLAMFLIHYVQRTLVFPVLIRGGKPTL-------LVTFVLAFLFCTFNGYVQSRYLSQF 146

Query: 142 VDYDGDGWFWWRFFGGGVVFLSGMAVNIWSDSVLVGLKSAG-GGYKLPTGGWFEFVSCPN 201
             Y  D      F  G  ++L GM +NI SD +L  L+  G  GYK+P GG FE+VS  N
Sbjct: 147 AVYAEDWVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSAAN 206

Query: 202 YLGEIVEWLGWAVMCGSWAGLSFFLYTCANLVPRARANHTWYLNKFGEDYPKCRKAVIPF 259
           Y GE+VEW G+A+   S  G+ F L+T + L+ RA+ +H WY  KF EDYPK RK +IPF
Sbjct: 207 YFGELVEWCGFALASWSLQGVVFALFTLSTLLTRAKQHHQWYHEKF-EDYPKSRKILIPF 258

[Close]

BLAST of Tan0021690 vs. ExPASy Swiss-Prot
Match: Q68FF9 (3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 SV=2)

HSP 1 Score: 153.7 bits (387), Expect = 2.9e-36
Identity = 94/238 (39.50%), Postives = 133/238 (55.88%), Query Frame = 0


Query: 24  VGLRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTL-W-LTLLLFPFGDHSSDPKPLFLI 83
           VGL+ + + YG+++    G  + A  AWFL E P++ W L   + P      +     L+
Sbjct: 25  VGLQMVGSSYGRYSSQWSGRRVPARPAWFLQELPSMAWPLYECIRPAAARLGNLPNRVLL 84

Query: 84  SPFLLHYLNRTFLYPIRLLWTGPRRNTAAGFPVVVALMAFGFNLLNSYLQTRSVSHYVDY 143
           + FL+HY+ RT ++P+ +    P         +   ++AF F  LN YLQ+R +S +  Y
Sbjct: 85  AMFLIHYVQRTLVFPVLIRGGKPTL-------LFTFVLAFLFCTLNGYLQSRYLSQFAVY 144

Query: 144 DGDGWFWWRFFGGGVVFLSGMAVNIWSDSVLVGLKSAG-GGYKLPTGGWFEFVSCPNYLG 203
             D      F  G  ++L GM +NI SD +L  L+  G  GYK+P GG FE+VS  NY G
Sbjct: 145 AEDWVTHPCFLTGFALWLVGMVINIHSDHILRNLRKPGETGYKIPRGGLFEYVSSANYFG 204

Query: 204 EIVEWLGWAVMCGSWAGLSFFLYTCANLVPRARANHTWYLNKFGEDYPKCRKAVIPFL 259
           E+VEW G+A+   S  G+ F L+T   L  RAR +H WYL KF EDYPK RK +IPFL
Sbjct: 205 ELVEWCGFALASWSLQGVVFALFTLCALFTRARQHHQWYLEKF-EDYPKTRKILIPFL 254

[Close]

BLAST of Tan0021690 vs. NCBI nr
Match: XP_038905208.1 (LOW QUALITY PROTEIN: steroid 5-alpha-reductase DET2 [Benincasa hispida])

HSP 1 Score: 489.6 bits (1259), Expect = 1.7e-134
Identity = 226/259 (87.26%), Postives = 239/259 (92.28%), Query Frame = 0


Query: 1   MGSDQALFHNSLLALFLIAPPTVVGLRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 60
           MGSDQALFHNSLL L+LI P  VV LRFLQAPYGKHN+PGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGSDQALFHNSLLRLYLIGPRQVVALRFLQAPYGKHNQPGWGPTMSAPLAWFLMESPTLW 60

Query: 61  LTLLLFPFGDHSSDPKPLFLISPFLLHYLNRTFLYPIRLLWTGPRRNTAAGFPVVVALMA 120
           LTLLLFP GDHSS+ K L LISPFLLHY NRT LYPIRLL +G RRNTAAGFPV+VA+MA
Sbjct: 61  LTLLLFPSGDHSSNSKALLLISPFLLHYFNRTCLYPIRLLRSGTRRNTAAGFPVIVAVMA 120

Query: 121 FGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFFGGGVVFLSGMAVNIWSDSVLVGLKSAGG 180
           FGFNLLNSYLQTRSVSHY++Y+GDGWFWWRFFGGGVVFL GM+VNIWSD VLVGLK  GG
Sbjct: 121 FGFNLLNSYLQTRSVSHYMEYEGDGWFWWRFFGGGVVFLLGMSVNIWSDGVLVGLKRGGG 180

Query: 181 GYKLPTGGWFEFVSCPNYLGEIVEWLGWAVMCGSWAGLSFFLYTCANLVPRARANHTWYL 240
           GYK+PTG WFE VSCPNYLGEIVEWLGWAVMCGSWAGL+FFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPTGWWFELVSCPNYLGEIVEWLGWAVMCGSWAGLAFFLYTCANLVPRARANHTWYL 240

Query: 241 NKFGEDYPKCRKAVIPFLY 260
           NKFGEDYP+ RKAVIPFLY
Sbjct: 241 NKFGEDYPRNRKAVIPFLY 259

[Close]

BLAST of Tan0021690 vs. NCBI nr
Match: KAA0044147.1 (steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa] >TYK24990.1 steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa])

HSP 1 Score: 488.0 bits (1255), Expect = 4.9e-134
Identity = 224/259 (86.49%), Postives = 235/259 (90.73%), Query Frame = 0


Query: 1   MGSDQALFHNSLLALFLIAPPTVVGLRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 60
           MG DQALF NSLLAL+LI PPTVV LRFLQAPYGKH RPGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 60

Query: 61  LTLLLFPFGDHSSDPKPLFLISPFLLHYLNRTFLYPIRLLWTGPRRNTAAGFPVVVALMA 120
           LTLLLFP GDHSSD K L LISPFLLHYLNRT  YPIRLLW+G RRN  AGFPV+VA+MA
Sbjct: 61  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 120

Query: 121 FGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFFGGGVVFLSGMAVNIWSDSVLVGLKSAGG 180
           FGFNLLN+Y+QTRSVSHYV Y+GDGWFWWRFFGGGVVF+ GMAVNIWSD VLVGLK +GG
Sbjct: 121 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 180

Query: 181 GYKLPTGGWFEFVSCPNYLGEIVEWLGWAVMCGSWAGLSFFLYTCANLVPRARANHTWYL 240
           GYK+P GGWFE VSCPNYLGEI+EWLGW VMCGSWAGL FFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 241 NKFGEDYPKCRKAVIPFLY 260
           NKFGEDYP  RKAVIPFLY
Sbjct: 241 NKFGEDYPINRKAVIPFLY 259

[Close]

BLAST of Tan0021690 vs. NCBI nr
Match: XP_008442471.1 (PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis melo])

HSP 1 Score: 488.0 bits (1255), Expect = 4.9e-134
Identity = 224/259 (86.49%), Postives = 235/259 (90.73%), Query Frame = 0


Query: 1   MGSDQALFHNSLLALFLIAPPTVVGLRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 60
           MG DQALF NSLLAL+LI PPTVV LRFLQAPYGKH RPGWGPTMSAPLAWFLMESPTLW
Sbjct: 33  MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92

Query: 61  LTLLLFPFGDHSSDPKPLFLISPFLLHYLNRTFLYPIRLLWTGPRRNTAAGFPVVVALMA 120
           LTLLLFP GDHSSD K L LISPFLLHYLNRT  YPIRLLW+G RRN  AGFPV+VA+MA
Sbjct: 93  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 152

Query: 121 FGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFFGGGVVFLSGMAVNIWSDSVLVGLKSAGG 180
           FGFNLLN+Y+QTRSVSHYV Y+GDGWFWWRFFGGGVVF+ GMAVNIWSD VLVGLK +GG
Sbjct: 153 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 212

Query: 181 GYKLPTGGWFEFVSCPNYLGEIVEWLGWAVMCGSWAGLSFFLYTCANLVPRARANHTWYL 240
           GYK+P GGWFE VSCPNYLGEI+EWLGW VMCGSWAGL FFLYTCANLVPRARANHTWYL
Sbjct: 213 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272

Query: 241 NKFGEDYPKCRKAVIPFLY 260
           NKFGEDYP  RKAVIPFLY
Sbjct: 273 NKFGEDYPINRKAVIPFLY 291

[Close]

BLAST of Tan0021690 vs. NCBI nr
Match: XP_004137737.1 (steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN58798.1 hypothetical protein Csa_002591 [Cucumis sativus])

HSP 1 Score: 486.5 bits (1251), Expect = 1.4e-133
Identity = 225/259 (86.87%), Postives = 234/259 (90.35%), Query Frame = 0


Query: 1   MGSDQALFHNSLLALFLIAPPTVVGLRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 60
           MG DQALFHNSLLAL+LI PPTVV LRFLQAPYGKH RPGWGPTMSAPLAWFLMESPTLW
Sbjct: 33  MGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92

Query: 61  LTLLLFPFGDHSSDPKPLFLISPFLLHYLNRTFLYPIRLLWTGPRRNTAAGFPVVVALMA 120
           LTLLLFP GDHSS  K L LISPFLLHYLNRT  YPIRLLW+G RRN   GFPV+VA+MA
Sbjct: 93  LTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNAQVGFPVLVAIMA 152

Query: 121 FGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFFGGGVVFLSGMAVNIWSDSVLVGLKSAGG 180
           FGFNLLNSY+QTRSVSHYV Y+GDGWFWWRFFGGGVVF+ GMAVNIWSD VLVGLK  GG
Sbjct: 153 FGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGG 212

Query: 181 GYKLPTGGWFEFVSCPNYLGEIVEWLGWAVMCGSWAGLSFFLYTCANLVPRARANHTWYL 240
           GYK+PTGGWFE VSCPNYLGEI+EWLGWAVMCGSWAGL FFLYTCANLVPRARANHTWYL
Sbjct: 213 GYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272

Query: 241 NKFGEDYPKCRKAVIPFLY 260
           NKFGE YP  RKAVIPFLY
Sbjct: 273 NKFGEVYPINRKAVIPFLY 291

[Close]

BLAST of Tan0021690 vs. NCBI nr
Match: XP_023527474.1 (steroid 5-alpha-reductase DET2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 481.5 bits (1238), Expect = 4.6e-132
Identity = 226/259 (87.26%), Postives = 234/259 (90.35%), Query Frame = 0


Query: 1   MGSDQALFHNSLLALFLIAPPTVVGLRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 60
           M SDQ LFHNSLLAL+LI PPT V LRFLQAPYGKHNRPGWGPTMS PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHNSLLALYLIGPPTAVSLRFLQAPYGKHNRPGWGPTMSVPLAWFLMESPTLW 60

Query: 61  LTLLLFPFGDHSSDPKPLFLISPFLLHYLNRTFLYPIRLLWTGPRRNTAAGFPVVVALMA 120
           LTLLLFP GD+SS+ K L LIS FLLHYLNRT LYPIRLL +G RRN+A GFPVVVALMA
Sbjct: 61  LTLLLFPSGDNSSNYKSLLLISLFLLHYLNRTCLYPIRLLRSGTRRNSATGFPVVVALMA 120

Query: 121 FGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFFGGGVVFLSGMAVNIWSDSVLVGLKSAGG 180
           FGFNLLNSYLQTRSVSHYVDYD D WFWWRFFGG VVF+ GMAVNIWSD VLV LK AGG
Sbjct: 121 FGFNLLNSYLQTRSVSHYVDYDRDEWFWWRFFGGAVVFVLGMAVNIWSDGVLVKLKMAGG 180

Query: 181 GYKLPTGGWFEFVSCPNYLGEIVEWLGWAVMCGSWAGLSFFLYTCANLVPRARANHTWYL 240
           GYK+PTGGWFE VSCPNYLGEIVEWLGWAVMCGSWAGL FFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPTGGWFELVSCPNYLGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 241 NKFGEDYPKCRKAVIPFLY 260
           NKFGEDYP+ RKAVIPFLY
Sbjct: 241 NKFGEDYPRDRKAVIPFLY 259

[Close]

BLAST of Tan0021690 vs. ExPASy TrEMBL
Match: A0A5D3DNY2 (Steroid 5-alpha-reductase DET2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G001080 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 2.4e-134
Identity = 224/259 (86.49%), Postives = 235/259 (90.73%), Query Frame = 0


Query: 1   MGSDQALFHNSLLALFLIAPPTVVGLRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 60
           MG DQALF NSLLAL+LI PPTVV LRFLQAPYGKH RPGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 60

Query: 61  LTLLLFPFGDHSSDPKPLFLISPFLLHYLNRTFLYPIRLLWTGPRRNTAAGFPVVVALMA 120
           LTLLLFP GDHSSD K L LISPFLLHYLNRT  YPIRLLW+G RRN  AGFPV+VA+MA
Sbjct: 61  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 120

Query: 121 FGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFFGGGVVFLSGMAVNIWSDSVLVGLKSAGG 180
           FGFNLLN+Y+QTRSVSHYV Y+GDGWFWWRFFGGGVVF+ GMAVNIWSD VLVGLK +GG
Sbjct: 121 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 180

Query: 181 GYKLPTGGWFEFVSCPNYLGEIVEWLGWAVMCGSWAGLSFFLYTCANLVPRARANHTWYL 240
           GYK+P GGWFE VSCPNYLGEI+EWLGW VMCGSWAGL FFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 241 NKFGEDYPKCRKAVIPFLY 260
           NKFGEDYP  RKAVIPFLY
Sbjct: 241 NKFGEDYPINRKAVIPFLY 259

[Close]

BLAST of Tan0021690 vs. ExPASy TrEMBL
Match: A0A1S3B6E3 (Steroid 5-alpha-reductase DET2 OS=Cucumis melo OX=3656 GN=LOC103486328 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 2.4e-134
Identity = 224/259 (86.49%), Postives = 235/259 (90.73%), Query Frame = 0


Query: 1   MGSDQALFHNSLLALFLIAPPTVVGLRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 60
           MG DQALF NSLLAL+LI PPTVV LRFLQAPYGKH RPGWGPTMSAPLAWFLMESPTLW
Sbjct: 33  MGWDQALFRNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92

Query: 61  LTLLLFPFGDHSSDPKPLFLISPFLLHYLNRTFLYPIRLLWTGPRRNTAAGFPVVVALMA 120
           LTLLLFP GDHSSD K L LISPFLLHYLNRT  YPIRLLW+G RRN  AGFPV+VA+MA
Sbjct: 93  LTLLLFPSGDHSSDSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNATAGFPVIVAIMA 152

Query: 121 FGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFFGGGVVFLSGMAVNIWSDSVLVGLKSAGG 180
           FGFNLLN+Y+QTRSVSHYV Y+GDGWFWWRFFGGGVVF+ GMAVNIWSD VLVGLK +GG
Sbjct: 153 FGFNLLNAYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRSGG 212

Query: 181 GYKLPTGGWFEFVSCPNYLGEIVEWLGWAVMCGSWAGLSFFLYTCANLVPRARANHTWYL 240
           GYK+P GGWFE VSCPNYLGEI+EWLGW VMCGSWAGL FFLYTCANLVPRARANHTWYL
Sbjct: 213 GYKVPKGGWFELVSCPNYLGEIMEWLGWGVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272

Query: 241 NKFGEDYPKCRKAVIPFLY 260
           NKFGEDYP  RKAVIPFLY
Sbjct: 273 NKFGEDYPINRKAVIPFLY 291

[Close]

BLAST of Tan0021690 vs. ExPASy TrEMBL
Match: A0A0A0LAB3 (Steroid 5-alpha-reductase DET2 OS=Cucumis sativus OX=3659 GN=Csa_3G732550 PE=3 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 6.9e-134
Identity = 225/259 (86.87%), Postives = 234/259 (90.35%), Query Frame = 0


Query: 1   MGSDQALFHNSLLALFLIAPPTVVGLRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 60
           MG DQALFHNSLLAL+LI PPTVV LRFLQAPYGKH RPGWGPTMSAPLAWFLMESPTLW
Sbjct: 33  MGWDQALFHNSLLALYLIGPPTVVSLRFLQAPYGKHIRPGWGPTMSAPLAWFLMESPTLW 92

Query: 61  LTLLLFPFGDHSSDPKPLFLISPFLLHYLNRTFLYPIRLLWTGPRRNTAAGFPVVVALMA 120
           LTLLLFP GDHSS  K L LISPFLLHYLNRT  YPIRLLW+G RRN   GFPV+VA+MA
Sbjct: 93  LTLLLFPSGDHSSHSKALLLISPFLLHYLNRTCFYPIRLLWSGTRRNAQVGFPVLVAIMA 152

Query: 121 FGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFFGGGVVFLSGMAVNIWSDSVLVGLKSAGG 180
           FGFNLLNSY+QTRSVSHYV Y+GDGWFWWRFFGGGVVF+ GMAVNIWSD VLVGLK  GG
Sbjct: 153 FGFNLLNSYVQTRSVSHYVKYEGDGWFWWRFFGGGVVFVMGMAVNIWSDGVLVGLKRRGG 212

Query: 181 GYKLPTGGWFEFVSCPNYLGEIVEWLGWAVMCGSWAGLSFFLYTCANLVPRARANHTWYL 240
           GYK+PTGGWFE VSCPNYLGEI+EWLGWAVMCGSWAGL FFLYTCANLVPRARANHTWYL
Sbjct: 213 GYKVPTGGWFELVSCPNYLGEIMEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 272

Query: 241 NKFGEDYPKCRKAVIPFLY 260
           NKFGE YP  RKAVIPFLY
Sbjct: 273 NKFGEVYPINRKAVIPFLY 291

[Close]

BLAST of Tan0021690 vs. ExPASy TrEMBL
Match: A0A6J1CX04 (Steroid 5-alpha-reductase DET2 OS=Momordica charantia OX=3673 GN=LOC111015065 PE=3 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 5.0e-132
Identity = 223/259 (86.10%), Postives = 234/259 (90.35%), Query Frame = 0


Query: 1   MGSDQALFHNSLLALFLIAPPTVVGLRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 60
           MGSDQALF NSLLAL+LI PPT V LRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW
Sbjct: 1   MGSDQALFRNSLLALYLIGPPTAVALRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 60

Query: 61  LTLLLFPFGDHSSDPKPLFLISPFLLHYLNRTFLYPIRLLWTGPRRNTAAGFPVVVALMA 120
           LTLLLFPFGDHSS+PKP+ LISPFLLHYL+RT LYPIRLL    +RN+AAGFPV VALMA
Sbjct: 61  LTLLLFPFGDHSSEPKPILLISPFLLHYLHRTCLYPIRLL---RQRNSAAGFPVAVALMA 120

Query: 121 FGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFFGGGVVFLSGMAVNIWSDSVLVGLKSAGG 180
           FGFN+LNSYLQTRSVSHY  Y+GDGWFWWRF  G VVF +GM VNIWSD VLVGLK AGG
Sbjct: 121 FGFNVLNSYLQTRSVSHYKSYEGDGWFWWRFSVGAVVFATGMGVNIWSDGVLVGLKRAGG 180

Query: 181 GYKLPTGGWFEFVSCPNYLGEIVEWLGWAVMCGSWAGLSFFLYTCANLVPRARANHTWYL 240
           GYKLPTGGWFE VSCPNYLGE+VEWLGWAVMC SWAGL FFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKLPTGGWFELVSCPNYLGEVVEWLGWAVMCWSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 241 NKFGEDYPKCRKAVIPFLY 260
           NKFGEDYP  RKA+IPFLY
Sbjct: 241 NKFGEDYPTTRKAIIPFLY 256

[Close]

BLAST of Tan0021690 vs. ExPASy TrEMBL
Match: A0A6J1J7F2 (Steroid 5-alpha-reductase DET2 OS=Cucurbita maxima OX=3661 GN=LOC111481932 PE=3 SV=1)

HSP 1 Score: 476.5 bits (1225), Expect = 7.2e-131
Identity = 223/259 (86.10%), Postives = 233/259 (89.96%), Query Frame = 0


Query: 1   MGSDQALFHNSLLALFLIAPPTVVGLRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLW 60
           M SDQ LFHNSLLAL+LI PPT + LRFLQAPYGKHNRPGWGPTMS PLAWFLMESPTLW
Sbjct: 1   MASDQTLFHNSLLALYLIGPPTAISLRFLQAPYGKHNRPGWGPTMSVPLAWFLMESPTLW 60

Query: 61  LTLLLFPFGDHSSDPKPLFLISPFLLHYLNRTFLYPIRLLWTGPRRNTAAGFPVVVALMA 120
           LTLLLFP GD+SS+ K L LIS FLLHYLNRT LYPIRLL +G RRN+A GFPVVVALMA
Sbjct: 61  LTLLLFPSGDNSSNYKSLLLISLFLLHYLNRTCLYPIRLLRSGTRRNSATGFPVVVALMA 120

Query: 121 FGFNLLNSYLQTRSVSHYVDYDGDGWFWWRFFGGGVVFLSGMAVNIWSDSVLVGLKSAGG 180
           F FNLLNSYLQTRSVSHY+DYD D WFWWRFFGG VVF+ GMAVNIWSD VLV LK AGG
Sbjct: 121 FVFNLLNSYLQTRSVSHYMDYDRDEWFWWRFFGGAVVFVLGMAVNIWSDGVLVKLKMAGG 180

Query: 181 GYKLPTGGWFEFVSCPNYLGEIVEWLGWAVMCGSWAGLSFFLYTCANLVPRARANHTWYL 240
           GYK+PTGGWFE VSCPNYLGEIVEWLGWAVMCGSWAGL FFLYTCANLVPRARANHTWYL
Sbjct: 181 GYKVPTGGWFELVSCPNYLGEIVEWLGWAVMCGSWAGLGFFLYTCANLVPRARANHTWYL 240

Query: 241 NKFGEDYPKCRKAVIPFLY 260
           NKFGEDYP+ RKAVIPFLY
Sbjct: 241 NKFGEDYPRDRKAVIPFLY 259

[Close]

BLAST of Tan0021690 vs. TAIR 10
Match: AT2G38050.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 349.7 bits (896), Expect = 1.9e-96
Identity = 163/260 (62.69%), Postives = 193/260 (74.23%), Query Frame = 0


Query: 3   SDQALFHNSLLALFLIAPPTVVGLRFLQAPYGKHNRPGWGPTMSAPLAWFLMESPTLWLT 62
           +D+  F   LL L    PPT V L+FLQAPYGKHNR GWGPT+S P+AWF+MESPTLWLT
Sbjct: 5   ADKTFFRYCLLTLIFAGPPTAVLLKFLQAPYGKHNRTGWGPTVSPPIAWFVMESPTLWLT 64

Query: 63  LLLFPFGDHSSDPKPLFLISPFLLHYLNRTFLYPIRLLWTG-PRRNTAAGFPVVVALMAF 122
           LLLFPFG H+ +PK L L SP+L+HY +RT +YP+RL  +  P      GFP+ +A +AF
Sbjct: 65  LLLFPFGRHALNPKSLLLFSPYLIHYFHRTIIYPLRLFRSSFPAGKN--GFPITIAALAF 124

Query: 123 GFNLLNSYLQTRSVSHYV-DYDGDGWFWWRFFGGGVVFLSGMAVNIWSDSVLVGLKSAG- 182
            FNLLN Y+Q R VSHY  DY+   WFWWRF  G VVF++GM +NI SD  LV LK    
Sbjct: 125 TFNLLNGYIQARWVSHYKDDYEDGNWFWWRFVIGMVVFITGMYINITSDRTLVRLKKENR 184

Query: 183 GGYKLPTGGWFEFVSCPNYLGEIVEWLGWAVMCGSWAGLSFFLYTCANLVPRARANHTWY 242
           GGY +P GGWFE VSCPNY GE +EWLGWAVM  SWAG+ FFLYTC+NL PRARA+H WY
Sbjct: 185 GGYVIPRGGWFELVSCPNYFGEAIEWLGWAVMTWSWAGIGFFLYTCSNLFPRARASHKWY 244

Query: 243 LNKFGEDYPKCRKAVIPFLY 260
           + KF E+YPK RKAVIPF+Y
Sbjct: 245 IAKFKEEYPKTRKAVIPFVY 262

[Close]

BLAST of Tan0021690 vs. TAIR 10
Match: AT5G16010.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 75.9 bits (185), Expect = 5.4e-14
Identity = 40/108 (37.04%), Postives = 66/108 (61.11%), Query Frame = 0


Query: 154 GGVVFLSGMAVNIWSDSVLVGLKSAGG--GYKLPTGGWFEFVSCPNYLGEIVEWLGWAVM 213
           G V+F+ G+  N++   +L  L+   G   YK+P GG F+ + CP+YL EI+ +  + ++
Sbjct: 162 GVVMFVVGIVGNLYHHVLLAKLRKEDGKKEYKIPKGGLFDIIICPHYLFEILVFWSFFLI 221

Query: 214 CGSWAGLSFFLYTCANLVPRARANHTWYLNKFGEDYPKCRKAVIPFLY 260
             +    SF + T   L+ R+ A  TWYL+KF +D+PK  KA+IPF++
Sbjct: 222 SQTIYSFSFAMGTMLYLIGRSYATRTWYLSKF-DDFPKHIKALIPFVF 268

[Close]

BLAST of Tan0021690 vs. TAIR 10
Match: AT1G72590.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 73.6 bits (179), Expect = 2.7e-13
Identity = 44/112 (39.29%), Postives = 59/112 (52.68%), Query Frame = 0


Query: 154 GGVVFLSGMAVNIWSDSVLVGLK---SAGGGYKLPTGGWFEFVSCPNYLGEIVEWLGWAV 213
           GG +FL G        ++L  L+   S    Y +P G WFE VSCP++L EIV +LG  +
Sbjct: 211 GGAIFLWGWIHQRRCHAILGSLREYPSQAKEYIIPYGDWFEMVSCPHFLAEIVLYLGLLI 270

Query: 214 MCGSWAGLSFFL---YTCANLVPRARANHTWYLNKFGEDYPKCRKAVIPFLY 260
             G    +S +L   +  ANL   A   H WYL KF E+YP  R A+ P +Y
Sbjct: 271 SSGG-TDISIWLLFGFVAANLTYAAGETHRWYLQKF-ENYPASRHAIFPHVY 320

[Close]

BLAST of Tan0021690 vs. TAIR 10
Match: AT2G16530.1 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.3e-10
Identity = 41/114 (35.96%), Postives = 55/114 (48.25%), Query Frame = 0


Query: 154 GGVVFLSGMAVNIWSDSVLVGLK---SAGGGYKLPTGGWFEFVSCPNYLGEIVEWLGWAV 213
           GG +FL G        ++L  L+   S    Y +P G WF  VS P++L EIV + G  +
Sbjct: 234 GGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYAGLLI 293

Query: 214 MCGS-----WAGLSFFLYTCANLVPRARANHTWYLNKFGEDYPKCRKAVIPFLY 260
             G      W     F +  ANL   A   H WYL KF E+YP  R A+ P++Y
Sbjct: 294 ASGGTDITIWL---LFGFVAANLTYAAGETHRWYLRKF-ENYPANRHAIFPYVY 343

[Close]

BLAST of Tan0021690 vs. TAIR 10
Match: AT2G16530.2 (3-oxo-5-alpha-steroid 4-dehydrogenase family protein )

HSP 1 Score: 64.7 bits (156), Expect = 1.3e-10
Identity = 41/114 (35.96%), Postives = 55/114 (48.25%), Query Frame = 0


Query: 154 GGVVFLSGMAVNIWSDSVLVGLK---SAGGGYKLPTGGWFEFVSCPNYLGEIVEWLGWAV 213
           GG +FL G        ++L  L+   S    Y +P G WF  VS P++L EIV + G  +
Sbjct: 233 GGAIFLWGWIHQRRCHAILGSLRENPSQAKEYIIPYGDWFGMVSSPHFLAEIVLYAGLLI 292

Query: 214 MCGS-----WAGLSFFLYTCANLVPRARANHTWYLNKFGEDYPKCRKAVIPFLY 260
             G      W     F +  ANL   A   H WYL KF E+YP  R A+ P++Y
Sbjct: 293 ASGGTDITIWL---LFGFVAANLTYAAGETHRWYLRKF-ENYPANRHAIFPYVY 342

The following BLAST results are available for this feature:

BLAST of Tan0021690 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q2QDF6	1.8e-99	67.30	Steroid 5-alpha-reductase DET2 OS=Gossypium hirsutum OX=3635 GN=DET2 PE=1 SV=1	[more]
Q38944	2.7e-95	62.69	Steroid 5-alpha-reductase DET2 OS=Arabidopsis thaliana OX=3702 GN=DET2 PE=1 SV=2	[more]
Q5K2N1	1.5e-85	58.53	Steroid 5-alpha-reductase DET2 OS=Solanum lycopersicum OX=4081 GN=DET2 PE=1 SV=1	[more]
P24008	2.2e-36	38.59	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Rattus norvegicus OX=10116 GN=Srd5a1 ...	[more]
Q68FF9	2.9e-36	39.50	3-oxo-5-alpha-steroid 4-dehydrogenase 1 OS=Mus musculus OX=10090 GN=Srd5a1 PE=2 ...	[more]

BLAST of Tan0021690 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038905208.1	1.7e-134	87.26	LOW QUALITY PROTEIN: steroid 5-alpha-reductase DET2 [Benincasa hispida]	[more]
KAA0044147.1	4.9e-134	86.49	steroid 5-alpha-reductase DET2 [Cucumis melo var. makuwa] >TYK24990.1 steroid 5-...	[more]
XP_008442471.1	4.9e-134	86.49	PREDICTED: steroid 5-alpha-reductase DET2 [Cucumis melo]	[more]
XP_004137737.1	1.4e-133	86.87	steroid 5-alpha-reductase DET2 [Cucumis sativus] >KGN58798.1 hypothetical protei...	[more]
XP_023527474.1	4.6e-132	87.26	steroid 5-alpha-reductase DET2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Tan0021690 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DNY2	2.4e-134	86.49	Steroid 5-alpha-reductase DET2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s...	[more]
A0A1S3B6E3	2.4e-134	86.49	Steroid 5-alpha-reductase DET2 OS=Cucumis melo OX=3656 GN=LOC103486328 PE=3 SV=1	[more]
A0A0A0LAB3	6.9e-134	86.87	Steroid 5-alpha-reductase DET2 OS=Cucumis sativus OX=3659 GN=Csa_3G732550 PE=3 S...	[more]
A0A6J1CX04	5.0e-132	86.10	Steroid 5-alpha-reductase DET2 OS=Momordica charantia OX=3673 GN=LOC111015065 PE...	[more]
A0A6J1J7F2	7.2e-131	86.10	Steroid 5-alpha-reductase DET2 OS=Cucurbita maxima OX=3661 GN=LOC111481932 PE=3 ...	[more]

BLAST of Tan0021690 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38050.1	1.9e-96	62.69	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT5G16010.1	5.4e-14	37.04	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT1G72590.1	2.7e-13	39.29	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.1	1.3e-10	35.96	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]
AT2G16530.2	1.3e-10	35.96	3-oxo-5-alpha-steroid 4-dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PFAM	PF02544	Steroid_dh	coord: 112..259 e-value: 1.0E-46 score: 158.7
IPR001104	3-oxo-5-alpha-steroid 4-dehydrogenase, C-terminal	PROSITE	PS50244	S5A_REDUCTASE	coord: 154..233 score: 26.181702
IPR016636	3-oxo-5-alpha-steroid 4-dehydrogenase	PIRSF	PIRSF015596	5_alpha-SR2	coord: 1..259 e-value: 5.9E-86 score: 285.8
None	No IPR available	GENE3D	1.20.120.1630		coord: 78..258 e-value: 3.0E-13 score: 51.9
None	No IPR available	PANTHER	PTHR10556:SF53	STEROID 5-ALPHA-REDUCTASE DET2	coord: 3..259
IPR039357	3-oxo-5-alpha-steroid 4-dehydrogenase/very-long-chain enoyl-CoA reductase	PANTHER	PTHR10556	3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE	coord: 3..259

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0021690.1	Tan0021690.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0006629	lipid metabolic process
biological_process	GO:0008202	steroid metabolic process
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0003865	3-oxo-5-alpha-steroid 4-dehydrogenase activity
molecular_function	GO:0047751	cholestenone 5-alpha-reductase activity
molecular_function	GO:0016627	oxidoreductase activity, acting on the CH-CH group of donors