Tan0021527 (gene) Snake gourd v1

Overview
NameTan0021527
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptioniron-sulfur assembly protein IscA-like 1, mitochondrial
LocationLG04: 6094170 .. 6097236 (-)
RNA-Seq ExpressionTan0021527
SyntenyTan0021527
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAACCAAACAGAGAAAAATGTGAAAGTTTGGAGTAAATGAACCTTCGCGAGTGTATTTGCAGAGGAGAAAATTGATAGGCAGAAGGAGGATCGAGAAAAATGGCAGCTTCTTCGTCTTCTTTTTTAGCCGCAGCTTCTCAGAAGGTTGGAGCAGCCGCTCGCCGTCAAATTCTTACTCTAACGGAGAACGCTGCCGCTAGAATTCGACAACTTCTTCAACAGCGCCAACGTCCATTCCTTCGCCTTGGCGTCAAGGCGCGCGGTTGTAATGGCTTATCCTATACTCTCAACTACGCAGGTTCGTAATACCTAATGAATTTCAACTTTGCTTGATTGTAGGCGATTTCTATTTTACTCATTTTATGTCAGAATTTAAGGGTGTGATGAAATTTGTCGATTATCTATCCTCGTTTCCTTGAATCAGATGTTGCTTGCTGCTTGGATTTAATTCGAGATAAATAGGCATTTGATGTATTTGAGCGATATAACCTAATAGTTAAGAGGAGATGTTTTGAAATTGGAGAAACTGGAGTCCTTTAGAAGATGAATTCTTGATATTTCAACTGTTTCCAGCTCTAGTTACCAAGAATTACGATGTTGATTATGAAGGGAGATGTAGAAAAATTAGAAGGTTAAATTATTCTATAATTGTGATTAACCGGAATTGGGTCTGGGTACGGTGTGTAAGTTTTTCAAAGATGAGTAAAGTAAGCTTAACTTAACTCAACAGAATTTTGCATGTACCCTCGATCAAGAGGTCGCAAGTTCGAATTCTCCCAACCTTCAATGTTGTTGAAATCAAAAAAGTTGTTCGAAGATGAGCTGTTGAGTTCTTGCTAACCTTAATAAACGACAAATATGAAAGTTAAAACCGACATGTCTGAATGCGTAAGTCGGTAGATTAGAGATAATTGACTTAACTAATAGGTCAGCCTGTAAATCATTCTGGATCTTATAGAATCAGATATGAATTATTGGTGTTATGCGACAGGAAGTTTGGATTCTATGATTTATTTCACAAACTTATGACATAGTGGGTTATTGATATATCACAATCCTGGACTAAATGCATGTTCCACCCAAATTTCTGGTGAACATTTGCTTTCATCAAGTACCATTTACTTTGTAGTGTAGGTTGGATATTGTGAGACCGTAGCGGGTGTGAACATCGATTAAATTGTGTTTGAGAGTGTTGTCTCTCTCTCTCCTGGTATGGTTAATTAGAATGTTATTGGACCAAAGTGCTGTTTCTATTCAATTAGAAGATAAATGGGTTAGCAAGCCTCCATGGGTCAACTTGGAGAGAATAGTTATTATGTTAGATGGTCAGAAGTTTGAACTTTTATTCACTTTGTGGCTTGTGATTTTTGATTGTTTGTGTTTATGTAATATGGCTATTTTCTCGGTTAACAGTATTTGCAATTCAGTTTTTTTATTTATTTATGAAGTCGCTTATGATTGAAGTTTCTAACTCAGCATTACCTCAGTTTTATAATCACATTATCATCAATTCCAGTTATTTTCCTTGTGCAAGAAGTAGGATGCGGTATTTATCTCTCTTTTGGCATAAATGGACTATAGTTATGATCTCTATCTTTTGAAATACTTCAAGATTGCAGTATTTCATATTAACGTTTTCTGTTTTCACATATTTTAGATGAGAAAGGGAAGTTTGATGAGTTGGTTGAGGATCAAGACGTGAAGATATTGATCGATCCAAAGGCTCTGATGCATGTAATAGGGACAAAGATGGACTTTGTAGATGACAAACTGAGGTATTTGAGAAATCTCTACCACAATTTTATATTTTCAGCTCTATTTCAATCCATGTGTTGAGGAAATATCATGCTTGTTAGTTACAAATTACAATATCAGACAGACATAAAAGCCTGCGTATTTATATTTAAATGTCGATGTGTAAGCATCTGCATCTGTATGTAAACCTAACGTTCCACATTTGTCTTGGCGCCTCGGCAACATTACTTCGTTATTCCAGAGCCTGAACATATTTTAATAGGTTTTAGTCAGGTGTGAGCCAACTCCATTCTGAATTTGCTATAGTCTGGTGGTATTTGCAACATGAAAGGATCACATAATTCATTTGCATCTGTGTTTGAGAATGGGTTCGGGTCATCTTTAGCATCCATAAGCTGGCAAATATAATTAATACCACTCGAGATAGAACTATTAATTTTAGGTCAAAATACTAATTTGGGTTGTTGTACTTTGGGCTTCATCCCATTTTAGTCTCTTAACTTTGAATAAAACATAAGGTGGGTCACTACTATTAGTTTATTGTCAACTTTTCTAAAAAATTAAACTTTTGTGTCACTGTTAGTCTTCCGTTTATAAATTTTGAATATATATTTACATGGTGTCTTTTCTTGCATTAAAATTATTATTATTTAACAAACTTCGATAAAAGTTAACTCCAAACTAGCAATAGAGACAATTTTAAGTTTTATTGAAAGTACAAGGTCTAAATTTGAATATTTGAAAAGTACAACAAATAAAAGGAATGAGATCCAAAGTACAAGGACTAAAATGGTACTTTAACTCTAATTTTATTAGAGTCAGATTATCTTGAATCTGGATGCTCAAATCAAATTCAACTTTTTAACCCATGAACTGCTATATTCAGTAGCCAATGAAACTTATTTTTTTCTCTCTCATTGTGGTAGATCTGAGTTTATCTTTATCAATCCAAATTCAAAAGGACAATGTGGCTGTGGGGAATCCTTTATGACAACAGGCAGTGCAGAAGCCAACAAAAAGTAAGGAGGAAGTTGAGTAATGAGCATTGCTTGAAAAACTCAAACTCACAGTTGCTGATTTGTTATGTATTTTGCTGAGAATACAAGGACTTTACCAAGAGAGTTCTCTGACAAGAACTTGAAACAGTTGTAAGAATGTTTCTATATGGCTAATTATTTTCTTAGAAAGCCAGTTAAGCTCTGTATTCTGCTAATTATTTTCTTTTGTTTTGTATGTATATTATTGTAGACTGCACTGACTAATGAGTTCTGAGGTAATAAAATTCTCCCACTACTTTCTTTTTTTTTCTA

mRNA sequence

GAAAACCAAACAGAGAAAAATGTGAAAGTTTGGAGTAAATGAACCTTCGCGAGTGTATTTGCAGAGGAGAAAATTGATAGGCAGAAGGAGGATCGAGAAAAATGGCAGCTTCTTCGTCTTCTTTTTTAGCCGCAGCTTCTCAGAAGGTTGGAGCAGCCGCTCGCCGTCAAATTCTTACTCTAACGGAGAACGCTGCCGCTAGAATTCGACAACTTCTTCAACAGCGCCAACGTCCATTCCTTCGCCTTGGCGTCAAGGCGCGCGGTTGTAATGGCTTATCCTATACTCTCAACTACGCAGATGAGAAAGGGAAGTTTGATGAGTTGGTTGAGGATCAAGACGTGAAGATATTGATCGATCCAAAGGCTCTGATGCATGTAATAGGGACAAAGATGGACTTTGTAGATGACAAACTGAGATCTGAGTTTATCTTTATCAATCCAAATTCAAAAGGACAATGTGGCTGTGGGGAATCCTTTATGACAACAGGCAGTGCAGAAGCCAACAAAAAGTAAGGAGGAAGTTGAGTAATGAGCATTGCTTGAAAAACTCAAACTCACAGTTGCTGATTTGTTATGTATTTTGCTGAGAATACAAGGACTTTACCAAGAGAGTTCTCTGACAAGAACTTGAAACAGTTGTAAGAATGTTTCTATATGGCTAATTATTTTCTTAGAAAGCCAGTTAAGCTCTGTATTCTGCTAATTATTTTCTTTTGTTTTGTATGTATATTATTGTAGACTGCACTGACTAATGAGTTCTGAGGTAATAAAATTCTCCCACTACTTTCTTTTTTTTTCTA

Coding sequence (CDS)

ATGGCAGCTTCTTCGTCTTCTTTTTTAGCCGCAGCTTCTCAGAAGGTTGGAGCAGCCGCTCGCCGTCAAATTCTTACTCTAACGGAGAACGCTGCCGCTAGAATTCGACAACTTCTTCAACAGCGCCAACGTCCATTCCTTCGCCTTGGCGTCAAGGCGCGCGGTTGTAATGGCTTATCCTATACTCTCAACTACGCAGATGAGAAAGGGAAGTTTGATGAGTTGGTTGAGGATCAAGACGTGAAGATATTGATCGATCCAAAGGCTCTGATGCATGTAATAGGGACAAAGATGGACTTTGTAGATGACAAACTGAGATCTGAGTTTATCTTTATCAATCCAAATTCAAAAGGACAATGTGGCTGTGGGGAATCCTTTATGACAACAGGCAGTGCAGAAGCCAACAAAAAGTAA

Protein sequence

MAASSSSFLAAASQKVGAAARRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQDVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTTGSAEANKK
Homology
BLAST of Tan0021527 vs. ExPASy Swiss-Prot
Match: Q8LBM4 (Iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g16710 PE=2 SV=2)

HSP 1 Score: 209.9 bits (533), Expect = 1.8e-53
Identity = 101/132 (76.52%), Postives = 120/132 (90.91%), Query Frame = 0

Query: 5   SSSFLAAASQKVGAAARRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S  LAAA+ +VG A R+Q+LTLT+ AA+R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YADEKGKFDELVEDQDVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGE 124
           YADEKGKFDELVE++ V+IL++PKALMHVIGTKMDFVDDKLRSEF+FINPNS+GQCGCGE
Sbjct: 63  YADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQGQCGCGE 122

Query: 125 SFMTTGSAEANK 137
           SFMTT ++ A +
Sbjct: 123 SFMTTSTSSAKQ 134

BLAST of Tan0021527 vs. ExPASy Swiss-Prot
Match: Q8L8C0 (Iron-sulfur assembly protein IscA-like 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g36260 PE=3 SV=2)

HSP 1 Score: 173.3 bits (438), Expect = 1.9e-42
Identity = 81/109 (74.31%), Postives = 99/109 (90.83%), Query Frame = 0

Query: 21  RRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQD 80
           R+Q+L L++ AAARIRQLLQ +Q+PFLRL V+A+GCNGLSY LNYA EKGKFDE+VE++ 
Sbjct: 2   RKQVLALSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKG 61

Query: 81  VKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTT 130
           VKIL+DPKA+MHVIGT+MDFVDDKLRSEF+F+NPN+  +CGCGESF TT
Sbjct: 62  VKILVDPKAVMHVIGTEMDFVDDKLRSEFVFVNPNAT-KCGCGESFTTT 109

BLAST of Tan0021527 vs. ExPASy Swiss-Prot
Match: Q07821 (Iron-sulfur assembly protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 4.5e-28
Identity = 58/107 (54.21%), Postives = 78/107 (72.90%), Query Frame = 0

Query: 21  RRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQD 80
           R+ ++TL+  A   +R LL Q +   +R+  + RGC+GL+Y L Y  E GKFDE+VE   
Sbjct: 143 RKALITLSPKAIKHLRALLAQPEPKLIRVSARNRGCSGLTYDLQYITEPGKFDEVVEQDG 202

Query: 81  VKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFM 128
           VKI+ID KAL  +IG++MD++DDKL S+F+F NPNSKG CGCGESFM
Sbjct: 203 VKIVIDSKALFSIIGSEMDWIDDKLASKFVFKNPNSKGTCGCGESFM 249

BLAST of Tan0021527 vs. ExPASy Swiss-Prot
Match: Q54VS1 (Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=isca1 PE=3 SV=1)

HSP 1 Score: 122.5 bits (306), Expect = 3.8e-27
Identity = 55/118 (46.61%), Postives = 90/118 (76.27%), Query Frame = 0

Query: 15  KVGAAARRQILTLTENAAARIRQLLQQRQRP---FLRLGVKARGCNGLSYTLNYADEKGK 74
           K+G  +++ I ++T++A  R+++++  ++      LRLG+K RGC+G+SYTL++A +K K
Sbjct: 8   KLGKPSKKAIFSMTDSALKRVKEIMNDKKIDNCIGLRLGIKERGCSGMSYTLDFATQKNK 67

Query: 75  FDE-LVEDQDVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMT 129
           FDE +V D+D+ I++D KAL+ VIGT+MD++++ ++ EFIFINPN+   CGCGESF T
Sbjct: 68  FDETVVADKDINIIVDSKALLSVIGTEMDYIEEPIKKEFIFINPNATNTCGCGESFTT 125

BLAST of Tan0021527 vs. ExPASy Swiss-Prot
Match: Q8T3X9 (Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Drosophila melanogaster OX=7227 GN=MagR PE=1 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 2.4e-26
Identity = 61/106 (57.55%), Postives = 75/106 (70.75%), Query Frame = 0

Query: 22  RQILTLTENAAARIRQLLQQR-QRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQD 81
           R  LTLT  A  RI+ LLQ +     L++GV+ RGCNGLSYTL+YA +K K DE V    
Sbjct: 23  RAALTLTPAAVLRIKTLLQDKPDMVGLKVGVRQRGCNGLSYTLDYASQKDKLDEEVVQDG 82

Query: 82  VKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESF 127
           VK+ ID KA + ++GT+MDFV+ KL SEF+F NPN KG CGCGESF
Sbjct: 83  VKVFIDKKAQLSLLGTEMDFVESKLSSEFVFNNPNIKGTCGCGESF 128

BLAST of Tan0021527 vs. NCBI nr
Match: XP_038891787.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial isoform X1 [Benincasa hispida])

HSP 1 Score: 254.6 bits (649), Expect = 4.8e-64
Identity = 133/137 (97.08%), Postives = 134/137 (97.81%), Query Frame = 0

Query: 1   MAASSSSFLAAASQKVGAAARRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSF AAASQKVG AARRQILTLTE AAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQDVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQ+VKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTTGSAEANKK
Sbjct: 121 GCGESFMTTGSAEANKK 137

BLAST of Tan0021527 vs. NCBI nr
Match: XP_022956980.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita moschata])

HSP 1 Score: 253.1 bits (645), Expect = 1.4e-63
Identity = 132/137 (96.35%), Postives = 135/137 (98.54%), Query Frame = 0

Query: 1   MAASSSSFLAAASQKVGAAARRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFLAAASQKVG AARR+ILTLTE AAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFLAAASQKVGPAARRKILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQDVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQ+VKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTTGSAEANKK
Sbjct: 121 GCGESFMTTGSAEANKK 137

BLAST of Tan0021527 vs. NCBI nr
Match: XP_022987453.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima])

HSP 1 Score: 253.1 bits (645), Expect = 1.4e-63
Identity = 132/137 (96.35%), Postives = 134/137 (97.81%), Query Frame = 0

Query: 1   MAASSSSFLAAASQKVGAAARRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSF AAASQKVG AARRQILTLTE AAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 19  MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 78

Query: 61  YTLNYADEKGKFDELVEDQDVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQ+VKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 79  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 138

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTTGSAEANKK
Sbjct: 139 GCGESFMTTGSAEANKK 155

BLAST of Tan0021527 vs. NCBI nr
Match: KAG7032017.1 (Iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 252.7 bits (644), Expect = 1.8e-63
Identity = 131/137 (95.62%), Postives = 134/137 (97.81%), Query Frame = 0

Query: 1   MAASSSSFLAAASQKVGAAARRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSF AAASQKVG AARRQILTLTE AAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 19  MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 78

Query: 61  YTLNYADEKGKFDELVEDQDVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQ+VKILI+PKALMHVIGTKMDF+DDKLRSEFIFINPNSKGQC
Sbjct: 79  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFIDDKLRSEFIFINPNSKGQC 138

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTTGSAEANKK
Sbjct: 139 GCGESFMTTGSAEANKK 155

BLAST of Tan0021527 vs. NCBI nr
Match: XP_023539778.1 (iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 252.3 bits (643), Expect = 2.4e-63
Identity = 132/137 (96.35%), Postives = 134/137 (97.81%), Query Frame = 0

Query: 1   MAASSSSFLAAASQKVGAAARRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFLAAASQKVG AARRQILTLTE AAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFLAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQDVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQ+VKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTTGSAEA KK
Sbjct: 121 GCGESFMTTGSAEAKKK 137

BLAST of Tan0021527 vs. ExPASy TrEMBL
Match: A0A6J1JJH4 (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111484969 PE=4 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 6.8e-64
Identity = 132/137 (96.35%), Postives = 134/137 (97.81%), Query Frame = 0

Query: 1   MAASSSSFLAAASQKVGAAARRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSF AAASQKVG AARRQILTLTE AAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 19  MAASSSSFFAAASQKVGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 78

Query: 61  YTLNYADEKGKFDELVEDQDVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQ+VKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 79  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 138

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTTGSAEANKK
Sbjct: 139 GCGESFMTTGSAEANKK 155

BLAST of Tan0021527 vs. ExPASy TrEMBL
Match: A0A6J1GZ87 (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111458498 PE=4 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 6.8e-64
Identity = 132/137 (96.35%), Postives = 135/137 (98.54%), Query Frame = 0

Query: 1   MAASSSSFLAAASQKVGAAARRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSFLAAASQKVG AARR+ILTLTE AAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFLAAASQKVGPAARRKILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQDVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQ+VKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTTGSAEANKK
Sbjct: 121 GCGESFMTTGSAEANKK 137

BLAST of Tan0021527 vs. ExPASy TrEMBL
Match: A0A6J1D9T8 (iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111018687 PE=4 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.6e-63
Identity = 130/137 (94.89%), Postives = 133/137 (97.08%), Query Frame = 0

Query: 1   MAASSSSFLAAASQKVGAAARRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSF AAAS+KVG AARRQ+LTLTE AA RIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASKKVGPAARRQVLTLTEAAAERIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQDVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQDVKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQDVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTTGSAEANKK
Sbjct: 121 GCGESFMTTGSAEANKK 137

BLAST of Tan0021527 vs. ExPASy TrEMBL
Match: A0A5A7SVZ7 (Iron-sulfur assembly protein IscA-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G001970 PE=4 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 9.9e-63
Identity = 130/137 (94.89%), Postives = 131/137 (95.62%), Query Frame = 0

Query: 1   MAASSSSFLAAASQKVGAAARRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSF AAASQK G A RRQILTLTE AAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKAGPAVRRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQDVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQ+VKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQNVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTT SAEANKK
Sbjct: 121 GCGESFMTTSSAEANKK 137

BLAST of Tan0021527 vs. ExPASy TrEMBL
Match: A0A0A0KTF0 (Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G614650 PE=4 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 9.9e-63
Identity = 130/137 (94.89%), Postives = 132/137 (96.35%), Query Frame = 0

Query: 1   MAASSSSFLAAASQKVGAAARRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60
           MAASSSSF AAASQK G AARRQILTLTE AAARIRQLLQQRQRPFLRLGVKARGCNGLS
Sbjct: 1   MAASSSSFFAAASQKAGPAARRQILTLTEAAAARIRQLLQQRQRPFLRLGVKARGCNGLS 60

Query: 61  YTLNYADEKGKFDELVEDQDVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120
           YTLNYADEKGKFDELVEDQ+VKILI+PKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC
Sbjct: 61  YTLNYADEKGKFDELVEDQNVKILIEPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQC 120

Query: 121 GCGESFMTTGSAEANKK 138
           GCGESFMTT SAEANKK
Sbjct: 121 GCGESFMTTSSAEANKK 137

BLAST of Tan0021527 vs. TAIR 10
Match: AT2G16710.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 209.9 bits (533), Expect = 1.3e-54
Identity = 101/132 (76.52%), Postives = 120/132 (90.91%), Query Frame = 0

Query: 5   SSSFLAAASQKVGAAARRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S  LAAA+ +VG A R+Q+LTLT+ AA+R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YADEKGKFDELVEDQDVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGE 124
           YADEKGKFDELVE++ V+IL++PKALMHVIGTKMDFVDDKLRSEF+FINPNS+GQCGCGE
Sbjct: 63  YADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQGQCGCGE 122

Query: 125 SFMTTGSAEANK 137
           SFMTT ++ A +
Sbjct: 123 SFMTTSTSSAKQ 134

BLAST of Tan0021527 vs. TAIR 10
Match: AT2G16710.3 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 203.4 bits (516), Expect = 1.2e-52
Identity = 101/138 (73.19%), Postives = 120/138 (86.96%), Query Frame = 0

Query: 5   SSSFLAAASQKVGAAARRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S  LAAA+ +VG A R+Q+LTLT+ AA+R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YA------DEKGKFDELVEDQDVKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKG 124
           YA      DEKGKFDELVE++ V+IL++PKALMHVIGTKMDFVDDKLRSEF+FINPNS+G
Sbjct: 63  YAGKTSGNDEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVFINPNSQG 122

Query: 125 QCGCGESFMTTGSAEANK 137
           QCGCGESFMTT ++ A +
Sbjct: 123 QCGCGESFMTTSTSSAKQ 140

BLAST of Tan0021527 vs. TAIR 10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 173.3 bits (438), Expect = 1.3e-43
Identity = 81/109 (74.31%), Postives = 99/109 (90.83%), Query Frame = 0

Query: 21  RRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLNYADEKGKFDELVEDQD 80
           R+Q+L L++ AAARIRQLLQ +Q+PFLRL V+A+GCNGLSY LNYA EKGKFDE+VE++ 
Sbjct: 2   RKQVLALSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKG 61

Query: 81  VKILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESFMTT 130
           VKIL+DPKA+MHVIGT+MDFVDDKLRSEF+F+NPN+  +CGCGESF TT
Sbjct: 62  VKILVDPKAVMHVIGTEMDFVDDKLRSEFVFVNPNAT-KCGCGESFTTT 109

BLAST of Tan0021527 vs. TAIR 10
Match: AT2G16710.2 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 160.2 bits (404), Expect = 1.2e-39
Identity = 79/102 (77.45%), Postives = 93/102 (91.18%), Query Frame = 0

Query: 5   SSSFLAAASQKVGAAARRQILTLTENAAARIRQLLQQRQRPFLRLGVKARGCNGLSYTLN 64
           +S  LAAA+ +VG A R+Q+LTLT+ AA+R+  LLQQRQ+PFLRLGVKARGCNGLSYTLN
Sbjct: 3   ASQILAAAAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYTLN 62

Query: 65  YADEKGKFDELVEDQDVKILIDPKALMHVIGTKMDFVDDKLR 107
           YADEKGKFDELVE++ V+IL++PKALMHVIGTKMDFVDDKLR
Sbjct: 63  YADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLR 104

BLAST of Tan0021527 vs. TAIR 10
Match: AT1G10500.1 (chloroplast-localized ISCA-like protein )

HSP 1 Score: 75.1 bits (183), Expect = 4.9e-14
Identity = 37/105 (35.24%), Postives = 63/105 (60.00%), Query Frame = 0

Query: 25  LTLTENAAARIRQLLQQRQRPF-LRLGVKARGCNGLSYTLNYADEKG--KFDELVEDQDV 84
           ++L+ENA   + ++  +R     LR+GVK  GC+G+SYT+++ +       D  +E Q  
Sbjct: 72  ISLSENALKHLSKMRSERGEDLCLRIGVKQGGCSGMSYTMDFENRANARPDDSTIEYQGF 131

Query: 85  KILIDPKALMHVIGTKMDFVDDKLRSEFIFINPNSKGQCGCGESF 127
            I+ DPK+++ + G ++D+ D  +   F F NPN+   CGCG+SF
Sbjct: 132 TIVCDPKSMLFLFGMQLDYSDALIGGGFSFSNPNATQTCGCGKSF 176

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LBM41.8e-5376.52Iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Arabidopsis thaliana ... [more]
Q8L8C01.9e-4274.31Iron-sulfur assembly protein IscA-like 3, mitochondrial OS=Arabidopsis thaliana ... [more]
Q078214.5e-2854.21Iron-sulfur assembly protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 /... [more]
Q54VS13.8e-2746.61Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Dictyostelium discoideu... [more]
Q8T3X92.4e-2657.55Iron-sulfur cluster assembly 1 homolog, mitochondrial OS=Drosophila melanogaster... [more]
Match NameE-valueIdentityDescription
XP_038891787.14.8e-6497.08iron-sulfur assembly protein IscA-like 1, mitochondrial isoform X1 [Benincasa hi... [more]
XP_022956980.11.4e-6396.35iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita moschata][more]
XP_022987453.11.4e-6396.35iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita maxima][more]
KAG7032017.11.8e-6395.62Iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita argyrosperma ... [more]
XP_023539778.12.4e-6396.35iron-sulfur assembly protein IscA-like 1, mitochondrial [Cucurbita pepo subsp. p... [more]
Match NameE-valueIdentityDescription
A0A6J1JJH46.8e-6496.35iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita maxima OX=3... [more]
A0A6J1GZ876.8e-6496.35iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Cucurbita moschata OX... [more]
A0A6J1D9T82.6e-6394.89iron-sulfur assembly protein IscA-like 1, mitochondrial OS=Momordica charantia O... [more]
A0A5A7SVZ79.9e-6394.89Iron-sulfur assembly protein IscA-like 1 OS=Cucumis melo var. makuwa OX=1194695 ... [more]
A0A0A0KTF09.9e-6394.89Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G614650... [more]
Match NameE-valueIdentityDescription
AT2G16710.11.3e-5476.52Iron-sulphur cluster biosynthesis family protein [more]
AT2G16710.31.2e-5273.19Iron-sulphur cluster biosynthesis family protein [more]
AT2G36260.11.3e-4374.31Iron-sulphur cluster biosynthesis family protein [more]
AT2G16710.21.2e-3977.45Iron-sulphur cluster biosynthesis family protein [more]
AT1G10500.14.9e-1435.24chloroplast-localized ISCA-like protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR035903HesB-like domain superfamilyGENE3D2.60.300.12coord: 24..130
e-value: 2.9E-34
score: 119.5
IPR035903HesB-like domain superfamilySUPERFAMILY89360HesB-like domaincoord: 24..117
IPR016092FeS cluster insertion proteinTIGRFAMTIGR00049TIGR00049coord: 25..127
e-value: 4.6E-35
score: 118.0
IPR000361FeS cluster biogenesisPFAMPF01521Fe-S_biosyncoord: 25..124
e-value: 3.4E-21
score: 75.5
NoneNo IPR availablePANTHERPTHR10072IRON-SULFUR CLUSTER ASSEMBLY PROTEINcoord: 1..136
NoneNo IPR availablePANTHERPTHR10072:SF60IRON-SULFUR ASSEMBLY PROTEIN ISCA-LIKE 3, MITOCHONDRIALcoord: 1..136
IPR017870FeS cluster insertion, C-terminal, conserved sitePROSITEPS01152HESBcoord: 109..126

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0021527.1Tan0021527.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016226 iron-sulfur cluster assembly
biological_process GO:0097428 protein maturation by iron-sulfur cluster transfer
cellular_component GO:0005739 mitochondrion
molecular_function GO:0051537 2 iron, 2 sulfur cluster binding
molecular_function GO:0051536 iron-sulfur cluster binding