Tan0021486 (gene) Snake gourd v1

Overview


Name	Tan0021486
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Calcium-binding EF-hand family protein
Location	LG11: 4247959 .. 4248996 (-)
RNA-Seq Expression	Tan0021486
Synteny	Tan0021486

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCCATTCAAAAACTTTCCAATTTCTTTAATTAAATTTTGACTTCGTACCCAAAACCCAAAATCCCCTGTTTCCTTCCACTTTCAAACGAATACGCGCAAACAGGGGCTTCAAATACCCACAATTCAATCCCCAATTCAACTCAAAAACATTCTCAATCAATCTGCAACCAACATTACCACCATTGAATTTGAATCCATCATCAATTTCTTCCCAATCCCACAATGGATTTCACTCTCTACAACGTCTCAATGGACGACAACAAAACAATCGCAGAGAATTCCTCTTTCCGCTTCCTCATCATGATCGACGGCTTTCGTTTTCAGAAACTCGCCTCTTGGCTCTACGGCTTCGCCTCCAGAAAGTCCTGGCGTTTCCCGATTCTAGAAACCCAAAATCATAAATCGGAGGACACGGCAGAGGACGGCGACGAGAGGCTGAGCAGAGAGGAGATGAAATCGGTGATGGGGAAATTGGGTATTTCTTGCGACGGCGATGAATTGGAGGAATCGCTTCCTCTGTTCGGATCAATGGAAGAGGTGTCGAAGATGTTCGATGAAAGGGCGCCGTGTGTGGCGGAGGTGAAGGAAGCTTTCGATGTGTTCGATGTAAACGGCGATGGGTTCATCGACGATGGAGAGTTGCAGAGAGTGCTCTGTGTTTTGGGGTTGAAAGAAGGGGAAGGGATTGAGAATTGCAGGAGGATGATTCAGAAGTTTGATGACAATAAAGATGGCAGAATTGATTTTGAGGAGTTTCTGAAGTTGATGGAGTTTGTGGTTTGAAGGATTCTTTTTTTTTTTTTAAATTCGATTATTGGTTTTTTTTTTTTTTAATTTTTGAATAATTCTTGTAGTGTGGTCGAAATTGAAGCCCTTTGCCCCTTCGATCTTGTTTTGTAAAGAAGCAAAGATCGAGATAGTTTCTTTGTTTTTTTTTGGGGGTATATGGAATTGAAATTTTGGCCCATCTTATTTCACTTTGTAATTTTTTATGTATACAAATATTTATAATTTTACCTTCTCTCTAAGTTTCTGTTC

mRNA sequence

CCCATTCAAAAACTTTCCAATTTCTTTAATTAAATTTTGACTTCGTACCCAAAACCCAAAATCCCCTGTTTCCTTCCACTTTCAAACGAATACGCGCAAACAGGGGCTTCAAATACCCACAATTCAATCCCCAATTCAACTCAAAAACATTCTCAATCAATCTGCAACCAACATTACCACCATTGAATTTGAATCCATCATCAATTTCTTCCCAATCCCACAATGGATTTCACTCTCTACAACGTCTCAATGGACGACAACAAAACAATCGCAGAGAATTCCTCTTTCCGCTTCCTCATCATGATCGACGGCTTTCGTTTTCAGAAACTCGCCTCTTGGCTCTACGGCTTCGCCTCCAGAAAGTCCTGGCGTTTCCCGATTCTAGAAACCCAAAATCATAAATCGGAGGACACGGCAGAGGACGGCGACGAGAGGCTGAGCAGAGAGGAGATGAAATCGGTGATGGGGAAATTGGGTATTTCTTGCGACGGCGATGAATTGGAGGAATCGCTTCCTCTGTTCGGATCAATGGAAGAGGTGTCGAAGATGTTCGATGAAAGGGCGCCGTGTGTGGCGGAGGTGAAGGAAGCTTTCGATGTGTTCGATGTAAACGGCGATGGGTTCATCGACGATGGAGAGTTGCAGAGAGTGCTCTGTGTTTTGGGGTTGAAAGAAGGGGAAGGGATTGAGAATTGCAGGAGGATGATTCAGAAGTTTGATGACAATAAAGATGGCAGAATTGATTTTGAGGAGTTTCTGAAGTTGATGGAGTTTGTGGTTTGAAGGATTCTTTTTTTTTTTTTAAATTCGATTATTGGTTTTTTTTTTTTTTAATTTTTGAATAATTCTTGTAGTGTGGTCGAAATTGAAGCCCTTTGCCCCTTCGATCTTGTTTTGTAAAGAAGCAAAGATCGAGATAGTTTCTTTGTTTTTTTTTGGGGGTATATGGAATTGAAATTTTGGCCCATCTTATTTCACTTTGTAATTTTTTATGTATACAAATATTTATAATTTTACCTTCTCTCTAAGTTTCTGTTC

Coding sequence (CDS)

ATGGATTTCACTCTCTACAACGTCTCAATGGACGACAACAAAACAATCGCAGAGAATTCCTCTTTCCGCTTCCTCATCATGATCGACGGCTTTCGTTTTCAGAAACTCGCCTCTTGGCTCTACGGCTTCGCCTCCAGAAAGTCCTGGCGTTTCCCGATTCTAGAAACCCAAAATCATAAATCGGAGGACACGGCAGAGGACGGCGACGAGAGGCTGAGCAGAGAGGAGATGAAATCGGTGATGGGGAAATTGGGTATTTCTTGCGACGGCGATGAATTGGAGGAATCGCTTCCTCTGTTCGGATCAATGGAAGAGGTGTCGAAGATGTTCGATGAAAGGGCGCCGTGTGTGGCGGAGGTGAAGGAAGCTTTCGATGTGTTCGATGTAAACGGCGATGGGTTCATCGACGATGGAGAGTTGCAGAGAGTGCTCTGTGTTTTGGGGTTGAAAGAAGGGGAAGGGATTGAGAATTGCAGGAGGATGATTCAGAAGTTTGATGACAATAAAGATGGCAGAATTGATTTTGAGGAGTTTCTGAAGTTGATGGAGTTTGTGGTTTGA

Protein sequence

MDFTLYNVSMDDNKTIAENSSFRFLIMIDGFRFQKLASWLYGFASRKSWRFPILETQNHKSEDTAEDGDERLSREEMKSVMGKLGISCDGDELEESLPLFGSMEEVSKMFDERAPCVAEVKEAFDVFDVNGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRIDFEEFLKLMEFVV

Homology

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BLAST of Tan0021486 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.1e-28
Identity = 67/124 (54.03%), Postives = 87/124 (70.16%), Query Frame = 0


Query: 60  KSEDTAEDGDERLSREEMKSVMGKLGISCDGDELEESLPLFGSMEEVSKMFDERAPCVAE 119
           K E+  +D D+ L RE++  VM  LG+S D +   E L    S +EVS +F+E+ P + E
Sbjct: 79  KHEEYRDDDDDGLCREDVGMVMKSLGLSTDQE--NEGLQKQYSSKEVSNLFEEKEPSLEE 138

Query: 120 VKEAFDVFDVNGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRIDFEEFL 179
           VK+AFDVFD N DGFID  +LQRVL +LGLK+G  +ENCRRMI+ FD +KDGRIDF  F+
Sbjct: 139 VKQAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFV 198

Query: 180 KLME 184
           K ME
Sbjct: 199 KFME 200

[Close]

BLAST of Tan0021486 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.3e-25
Identity = 62/122 (50.82%), Postives = 83/122 (68.03%), Query Frame = 0


Query: 62  EDTAEDGDERLSREEMKSVMGKLGISCDGDELEESLPLFGSMEEVSKMFDERAPCVAEVK 121
           +D  +D D  +SREE + VM  LG+  + D+L+E      S +EVS +F+E+   + EVK
Sbjct: 73  DDDDDDDDIDISREEAEMVMRSLGLFYNDDQLQEQY----SAKEVSSLFEEKEASLEEVK 132

Query: 122 EAFDVFDVNGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRIDFEEFLKL 181
           +AFDVFD N DGFID  ELQRVL +LG K+G  ++NC  MI+  D NKDG+IDF EF+K 
Sbjct: 133 QAFDVFDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKF 190

Query: 182 ME 184
           ME
Sbjct: 193 ME 190

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BLAST of Tan0021486 vs. ExPASy Swiss-Prot
Match: Q0JC44 (Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 GN=CML22 PE=3 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 6.3e-17
Identity = 53/139 (38.13%), Postives = 76/139 (54.68%), Query Frame = 0


Query: 67  DGDERLSREEMKSVMGKLGISCDGDELEESLPLFGS-------MEEVSKMF--------- 126
           +GD R++REE++  +GKLGI    DEL   +    +       +EE  +++         
Sbjct: 103 NGDGRITREELEDSLGKLGIPVPADELAAVIARIDANGDGCVDVEEFGELYRSIMAGGDD 162

Query: 127 -------DERAPCVAEVKEAFDVFDVNGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQ 183
                  +E      +++EAF VFD NGDG+I   EL  VL  LGLK+G   E CRRMI 
Sbjct: 163 SKDGRAKEEEEEEDGDMREAFRVFDANGDGYITVDELGAVLASLGLKQGRTAEECRRMIG 222

[Close]

BLAST of Tan0021486 vs. ExPASy Swiss-Prot
Match: Q9ZR02 (Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.0e-15
Identity = 48/131 (36.64%), Postives = 75/131 (57.25%), Query Frame = 0


Query: 66  EDGDERLSREEMKSVMGKLGISCDGDELEE-------------SLPLFGSMEEVSKMFDE 125
           +DGD +++ +E+      LGI    DEL +              +  FG + +   + DE
Sbjct: 15  KDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEFGELYKTIMVEDE 74

Query: 126 RAPCVAEVKEAFDVFDVNGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGR 184
                 ++KEAF+VFD NGDGFI   EL+ VL  LGLK+G+ +E CR+MI + D + DGR
Sbjct: 75  DEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECRKMIMQVDVDGDGR 134

[Close]

BLAST of Tan0021486 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 5.9e-15
Identity = 46/127 (36.22%), Postives = 76/127 (59.84%), Query Frame = 0


Query: 66  EDGDERLSREEMKSVMGKLGISCDGDELEESLPLF-----GSM--EEVSKMFDERAPCVA 125
           ++GD ++++ E+K     +GI    +E+ E +        G+M  +E   ++ E      
Sbjct: 15  KNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSLYQEMVEEKE 74

Query: 126 E---VKEAFDVFDVNGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRIDF 183
           E   ++EAF VFD NGDGFI D EL+ VL  +GLK+G  +E+C++MI K D + DG ++F
Sbjct: 75  EEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDVDGDGMVNF 134

[Close]

BLAST of Tan0021486 vs. NCBI nr
Match: XP_022133625.1 (probable calcium-binding protein CML45 [Momordica charantia])

HSP 1 Score: 224.2 bits (570), Expect = 9.5e-55
Identity = 119/187 (63.64%), Postives = 140/187 (74.87%), Query Frame = 0


Query: 1   MDFTLYNVSMDDNKTIAENSSFRFLIMIDGFRFQKLASWL----YGFASRKSWRFPILET 60
           MD  LYN+SM +     EN S RFLIMID   FQKL SWL    +GFAS ++        
Sbjct: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRN------SN 60

Query: 61  QNHKSEDTAEDGDERLSREEMKSVMGKLGISCDGDELEESLPLFGSMEEVSKMFDERAPC 120
           +         DG E +SREEMKSVMG LGI C G+E +ESLPLFGS+EE++K+F+ER PC
Sbjct: 61  RCSPIPKAVVDGGESVSREEMKSVMGDLGIVC-GEEGDESLPLFGSVEEIAKLFEERGPC 120

Query: 121 VAEVKEAFDVFDVNGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRIDFE 180
           +AEVK AFDVFD NGDGFID  ELQRVLCVLGLKEGEG+ENC+RMIQKFD+N+DG+IDFE
Sbjct: 121 IAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFE 180

Query: 181 EFLKLME 184
           EF+KLME
Sbjct: 181 EFVKLME 180

[Close]

BLAST of Tan0021486 vs. NCBI nr
Match: XP_038889669.1 (probable calcium-binding protein CML46 [Benincasa hispida])

HSP 1 Score: 221.9 bits (564), Expect = 4.7e-54
Identity = 125/187 (66.84%), Postives = 141/187 (75.40%), Query Frame = 0


Query: 1   MDFTLYNVSMDDNKTIAENSSFRFLIMIDGFRFQKLASWLYGFASRKSWRFPILETQNHK 60
           MDF +YNVSMDD KT  +N SFRFLI+ID  RFQKLASWLY FA+ K  + P       K
Sbjct: 1   MDFGVYNVSMDD-KTTTQNPSFRFLIVIDFLRFQKLASWLYAFATTK--QKPSSFPATSK 60

Query: 61  SEDTAEDGDERL-SREEMKSVMGKLGISCDGDELEESLPLFGSMEEVSKMFDERAPCVAE 120
           +E+    G+ERL + EEMK VMG LGI C     EES+PLFGS EEV++MFDERA    E
Sbjct: 61  TEE--HGGEERLMTTEEMKWVMGNLGIDCG----EESVPLFGSTEEVAEMFDERAASTVE 120

Query: 121 VKEAFDVFDVNGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRIDFEEFL 180
           VK+AFDVFDVNGDGFID  ELQRV+CVLG KEGE I+NC RMI+KFD N DGRIDFEEFL
Sbjct: 121 VKKAFDVFDVNGDGFIDSEELQRVMCVLGFKEGERIQNCERMIRKFDHNGDGRIDFEEFL 178

Query: 181 KLMEFVV 187
           KLME VV
Sbjct: 181 KLMESVV 178

[Close]

BLAST of Tan0021486 vs. NCBI nr
Match: KAG6578574.1 (putative calcium-binding protein CML46, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 221.1 bits (562), Expect = 8.1e-54
Identity = 117/177 (66.10%), Postives = 133/177 (75.14%), Query Frame = 0


Query: 12  DNKTIAENSSFRFLIMIDGFRFQKLASWLYGFAS--RKSWRFPILETQNHKSEDTAEDGD 71
           D  TI  N SFRFLIMID FRFQKL SWLY FA+  RK W  P  ET   +  D   +G 
Sbjct: 2   DGNTIPANPSFRFLIMIDCFRFQKLGSWLYAFATGKRKQWSLPASETGKSEQRD---EGG 61

Query: 72  ERLSREEMKSVMGKLGISCDGDELEESLPLFGSMEEVSKMFDERAPCVAEVKEAFDVFDV 131
           ER+SREEMK VMG+LG+S + +E EESLP F SMEE+ ++FD+ A   AEVKEAFDVFDV
Sbjct: 62  ERVSREEMKWVMGELGLSSE-EETEESLPGFASMEEMCRIFDQTAASAAEVKEAFDVFDV 121

Query: 132 NGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRIDFEEFLKLMEFVV 187
           N DGFID  ELQRVLCVLG KEGE I+NCRRMI KFD+N DGRIDF+EF+KLME  V
Sbjct: 122 NSDGFIDGDELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMESAV 174

[Close]

BLAST of Tan0021486 vs. NCBI nr
Match: XP_022993675.1 (probable calcium-binding protein CML30 [Cucurbita maxima])

HSP 1 Score: 220.3 bits (560), Expect = 1.4e-53
Identity = 122/177 (68.93%), Postives = 133/177 (75.14%), Query Frame = 0


Query: 12  DNKTIAENSSFRFLIMIDGFRFQKLASWLYGFAS--RKSWRFPILETQNHKSEDTAEDGD 71
           D  TI  N SFRFLIMID FRFQKL SWLY FAS  RK W  P  ET   KSE   E G 
Sbjct: 2   DGNTIPANPSFRFLIMIDCFRFQKLGSWLYAFASGKRKQWSLPASETA--KSEQRKE-GG 61

Query: 72  ERLSREEMKSVMGKLGISCDGDELEESLPLFGSMEEVSKMFDERAPCVAEVKEAFDVFDV 131
           ER+SREEMK VM +LG+S  G+E EESLP F SMEE+ K+FD+ A   AEVKEAFDVFDV
Sbjct: 62  ERVSREEMKWVMEELGLSW-GEETEESLPGFASMEEMCKIFDQTAASAAEVKEAFDVFDV 121

Query: 132 NGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRIDFEEFLKLMEFVV 187
           N DGFID  ELQRVLCVLG KEGE I+NCRRMI KFD+N DGRIDF+EF+KLME  V
Sbjct: 122 NSDGFIDVEELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMESAV 174

[Close]

BLAST of Tan0021486 vs. NCBI nr
Match: XP_022939128.1 (probable calcium-binding protein CML45 [Cucurbita moschata])

HSP 1 Score: 218.0 bits (554), Expect = 6.8e-53
Identity = 116/177 (65.54%), Postives = 132/177 (74.58%), Query Frame = 0


Query: 12  DNKTIAENSSFRFLIMIDGFRFQKLASWLYGFAS--RKSWRFPILETQNHKSEDTAEDGD 71
           D  TI    SFRFLIMID FRFQKL SWLY FA+  RK W  P  ET   +  D   +G 
Sbjct: 2   DGNTIPAIPSFRFLIMIDCFRFQKLGSWLYAFATGKRKQWSLPASETAKSEQRD---EGG 61

Query: 72  ERLSREEMKSVMGKLGISCDGDELEESLPLFGSMEEVSKMFDERAPCVAEVKEAFDVFDV 131
           ER+SREEMK VMG+LG+S + +E EESLP F SMEE+ ++FD+ A   AEVKEAFDVFDV
Sbjct: 62  ERVSREEMKWVMGELGLSSE-EETEESLPGFASMEEMCRIFDQTAASAAEVKEAFDVFDV 121

Query: 132 NGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRIDFEEFLKLMEFVV 187
           N DGFID  ELQRVLCVLG KEGE I+NCRRMI KFD+N DGRIDF+EF+KLME  V
Sbjct: 122 NSDGFIDGDELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMESAV 174

[Close]

BLAST of Tan0021486 vs. ExPASy TrEMBL
Match: A0A6J1BVS4 (probable calcium-binding protein CML45 OS=Momordica charantia OX=3673 GN=LOC111006164 PE=4 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 4.6e-55
Identity = 119/187 (63.64%), Postives = 140/187 (74.87%), Query Frame = 0


Query: 1   MDFTLYNVSMDDNKTIAENSSFRFLIMIDGFRFQKLASWL----YGFASRKSWRFPILET 60
           MD  LYN+SM +     EN S RFLIMID   FQKL SWL    +GFAS ++        
Sbjct: 1   MDLNLYNLSMAEKTDRPENPSLRFLIMIDCLVFQKLTSWLSATYHGFASTRN------SN 60

Query: 61  QNHKSEDTAEDGDERLSREEMKSVMGKLGISCDGDELEESLPLFGSMEEVSKMFDERAPC 120
           +         DG E +SREEMKSVMG LGI C G+E +ESLPLFGS+EE++K+F+ER PC
Sbjct: 61  RCSPIPKAVVDGGESVSREEMKSVMGDLGIVC-GEEGDESLPLFGSVEEIAKLFEERGPC 120

Query: 121 VAEVKEAFDVFDVNGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRIDFE 180
           +AEVK AFDVFD NGDGFID  ELQRVLCVLGLKEGEG+ENC+RMIQKFD+N+DG+IDFE
Sbjct: 121 IAEVKRAFDVFDANGDGFIDVVELQRVLCVLGLKEGEGVENCKRMIQKFDENEDGKIDFE 180

Query: 181 EFLKLME 184
           EF+KLME
Sbjct: 181 EFVKLME 180

[Close]

BLAST of Tan0021486 vs. ExPASy TrEMBL
Match: A0A6J1JZ70 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111489598 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 6.7e-54
Identity = 122/177 (68.93%), Postives = 133/177 (75.14%), Query Frame = 0


Query: 12  DNKTIAENSSFRFLIMIDGFRFQKLASWLYGFAS--RKSWRFPILETQNHKSEDTAEDGD 71
           D  TI  N SFRFLIMID FRFQKL SWLY FAS  RK W  P  ET   KSE   E G 
Sbjct: 2   DGNTIPANPSFRFLIMIDCFRFQKLGSWLYAFASGKRKQWSLPASETA--KSEQRKE-GG 61

Query: 72  ERLSREEMKSVMGKLGISCDGDELEESLPLFGSMEEVSKMFDERAPCVAEVKEAFDVFDV 131
           ER+SREEMK VM +LG+S  G+E EESLP F SMEE+ K+FD+ A   AEVKEAFDVFDV
Sbjct: 62  ERVSREEMKWVMEELGLSW-GEETEESLPGFASMEEMCKIFDQTAASAAEVKEAFDVFDV 121

Query: 132 NGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRIDFEEFLKLMEFVV 187
           N DGFID  ELQRVLCVLG KEGE I+NCRRMI KFD+N DGRIDF+EF+KLME  V
Sbjct: 122 NSDGFIDVEELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMESAV 174

[Close]

BLAST of Tan0021486 vs. ExPASy TrEMBL
Match: A0A6J1FKT4 (probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC111445127 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 3.3e-53
Identity = 116/177 (65.54%), Postives = 132/177 (74.58%), Query Frame = 0


Query: 12  DNKTIAENSSFRFLIMIDGFRFQKLASWLYGFAS--RKSWRFPILETQNHKSEDTAEDGD 71
           D  TI    SFRFLIMID FRFQKL SWLY FA+  RK W  P  ET   +  D   +G 
Sbjct: 2   DGNTIPAIPSFRFLIMIDCFRFQKLGSWLYAFATGKRKQWSLPASETAKSEQRD---EGG 61

Query: 72  ERLSREEMKSVMGKLGISCDGDELEESLPLFGSMEEVSKMFDERAPCVAEVKEAFDVFDV 131
           ER+SREEMK VMG+LG+S + +E EESLP F SMEE+ ++FD+ A   AEVKEAFDVFDV
Sbjct: 62  ERVSREEMKWVMGELGLSSE-EETEESLPGFASMEEMCRIFDQTAASAAEVKEAFDVFDV 121

Query: 132 NGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRIDFEEFLKLMEFVV 187
           N DGFID  ELQRVLCVLG KEGE I+NCRRMI KFD+N DGRIDF+EF+KLME  V
Sbjct: 122 NSDGFIDGDELQRVLCVLGFKEGEAIQNCRRMINKFDENNDGRIDFQEFVKLMESAV 174

[Close]

BLAST of Tan0021486 vs. ExPASy TrEMBL
Match: A0A1S3CAB3 (probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103498740 PE=4 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 6.9e-51
Identity = 116/186 (62.37%), Postives = 135/186 (72.58%), Query Frame = 0


Query: 1   MDFTLYNVSMDDNKTIAENSSFRFLIMIDGFRFQKLASWLYGFASRKSWRFPILETQNHK 60
           M+F LYNVSMDD K+  +N SFRFLI+ID  RFQ LASWL  FAS K  +   L   +  
Sbjct: 1   MNFGLYNVSMDD-KSTPQNPSFRFLIIIDFLRFQNLASWLCSFASTK-LKQSTLPASSKS 60

Query: 61  SEDTAEDGDERLSREEMKSVMGKLGISCDGDELEESLPLFGSMEEVSKMFDERAPCVAEV 120
            E +A + +  +S EEMK VMG LG+ C       S+PLFGS EEV++MFD+ A   AEV
Sbjct: 61  VESSAGERERLVSTEEMKWVMGNLGMDCSEG---SSVPLFGSAEEVAEMFDQTAASAAEV 120

Query: 121 KEAFDVFDVNGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRIDFEEFLK 180
           K+AFDVFDVN DGFID  ELQRV+CVLG KEGEGIENC +MI KFD NKDGRIDFEEF+K
Sbjct: 121 KQAFDVFDVNADGFIDVEELQRVMCVLGFKEGEGIENCEKMISKFDYNKDGRIDFEEFVK 180

Query: 181 LMEFVV 187
           LME VV
Sbjct: 181 LMEAVV 181

[Close]

BLAST of Tan0021486 vs. ExPASy TrEMBL
Match: A0A0A0KS94 (Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_5G636620 PE=4 SV=1)

HSP 1 Score: 203.4 bits (516), Expect = 8.4e-49
Identity = 116/187 (62.03%), Postives = 133/187 (71.12%), Query Frame = 0


Query: 1   MDFTLYNVSMDDNKTIAENSSFRFLIMIDGFRFQKLASWLYGFASRKSWRFPILETQNHK 60
           M+  LYNVSMDD K+  +N SFRFL++ID  RFQ LASWL    S K  +     T    
Sbjct: 2   MNLGLYNVSMDD-KSTPQNPSFRFLLIIDFLRFQNLASWLCSSTSTKLKQ----STPPAS 61

Query: 61  SEDTAEDGDERL-SREEMKSVMGKLGISCDGDELEESLPLFGSMEEVSKMFDERAPCVAE 120
           S+      +ERL S EEMK VMG LGI C       S+PLFGS EEV++MFD+ A  VAE
Sbjct: 62  SKSVESRAEERLVSTEEMKWVMGNLGIDCSEG---SSVPLFGSAEEVAEMFDQTAASVAE 121

Query: 121 VKEAFDVFDVNGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRIDFEEFL 180
           VK+AF VFDVNGDGFID  ELQRV+CVLG KEGEGIENC +MI+KFD NKDGRIDFEEF+
Sbjct: 122 VKQAFGVFDVNGDGFIDVEELQRVMCVLGFKEGEGIENCEKMIRKFDSNKDGRIDFEEFV 180

Query: 181 KLMEFVV 187
           KLME VV
Sbjct: 182 KLMEAVV 180

[Close]

BLAST of Tan0021486 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 127.1 bits (318), Expect = 1.5e-29
Identity = 67/124 (54.03%), Postives = 87/124 (70.16%), Query Frame = 0


Query: 60  KSEDTAEDGDERLSREEMKSVMGKLGISCDGDELEESLPLFGSMEEVSKMFDERAPCVAE 119
           K E+  +D D+ L RE++  VM  LG+S D +   E L    S +EVS +F+E+ P + E
Sbjct: 79  KHEEYRDDDDDGLCREDVGMVMKSLGLSTDQE--NEGLQKQYSSKEVSNLFEEKEPSLEE 138

Query: 120 VKEAFDVFDVNGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRIDFEEFL 179
           VK+AFDVFD N DGFID  +LQRVL +LGLK+G  +ENCRRMI+ FD +KDGRIDF  F+
Sbjct: 139 VKQAFDVFDENRDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGFV 198

Query: 180 KLME 184
           K ME
Sbjct: 199 KFME 200

[Close]

BLAST of Tan0021486 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 117.9 bits (294), Expect = 9.0e-27
Identity = 62/122 (50.82%), Postives = 83/122 (68.03%), Query Frame = 0


Query: 62  EDTAEDGDERLSREEMKSVMGKLGISCDGDELEESLPLFGSMEEVSKMFDERAPCVAEVK 121
           +D  +D D  +SREE + VM  LG+  + D+L+E      S +EVS +F+E+   + EVK
Sbjct: 73  DDDDDDDDIDISREEAEMVMRSLGLFYNDDQLQEQY----SAKEVSSLFEEKEASLEEVK 132

Query: 122 EAFDVFDVNGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRIDFEEFLKL 181
           +AFDVFD N DGFID  ELQRVL +LG K+G  ++NC  MI+  D NKDG+IDF EF+K 
Sbjct: 133 QAFDVFDENKDGFIDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEFVKF 190

Query: 182 ME 184
           ME
Sbjct: 193 ME 190

[Close]

BLAST of Tan0021486 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 84.0 bits (206), Expect = 1.4e-16
Identity = 48/131 (36.64%), Postives = 75/131 (57.25%), Query Frame = 0


Query: 66  EDGDERLSREEMKSVMGKLGISCDGDELEE-------------SLPLFGSMEEVSKMFDE 125
           +DGD +++ +E+      LGI    DEL +              +  FG + +   + DE
Sbjct: 15  KDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEFGELYKTIMVEDE 74

Query: 126 RAPCVAEVKEAFDVFDVNGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGR 184
                 ++KEAF+VFD NGDGFI   EL+ VL  LGLK+G+ +E CR+MI + D + DGR
Sbjct: 75  DEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGKTLEECRKMIMQVDVDGDGR 134

[Close]

BLAST of Tan0021486 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 82.4 bits (202), Expect = 4.2e-16
Identity = 46/127 (36.22%), Postives = 76/127 (59.84%), Query Frame = 0


Query: 66  EDGDERLSREEMKSVMGKLGISCDGDELEESLPLF-----GSM--EEVSKMFDERAPCVA 125
           ++GD ++++ E+K     +GI    +E+ E +        G+M  +E   ++ E      
Sbjct: 15  KNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEFGSLYQEMVEEKE 74

Query: 126 E---VKEAFDVFDVNGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRIDF 183
           E   ++EAF VFD NGDGFI D EL+ VL  +GLK+G  +E+C++MI K D + DG ++F
Sbjct: 75  EEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQGRTLEDCKKMISKVDVDGDGMVNF 134

[Close]

BLAST of Tan0021486 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 79.7 bits (195), Expect = 2.7e-15
Identity = 46/129 (35.66%), Postives = 80/129 (62.02%), Query Frame = 0


Query: 66  EDGDERLSREEMKSVMGKLGISCDGDELE---ESLPLFG----SMEEVSKMF----DERA 125
           ++GD  ++ +E+   +  LGI     EL    E + + G     ++E  +++    DE  
Sbjct: 15  KNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGELYKTIMDEED 74

Query: 126 PCVAEVKEAFDVFDVNGDGFIDDGELQRVLCVLGLKEGEGIENCRRMIQKFDDNKDGRID 184
               ++KEAF+VFD NGDGFI   EL+ VL  LGLK+G+ +++C++MI+K D + DGR++
Sbjct: 75  EEEEDMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVN 134

The following BLAST results are available for this feature:

BLAST of Tan0021486 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93Z27	2.1e-28	54.03	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9MBG5	1.3e-25	50.82	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q0JC44	6.3e-17	38.13	Probable calcium-binding protein CML22 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9ZR02	2.0e-15	36.64	Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1	[more]
Q9SU00	5.9e-15	36.22	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]

BLAST of Tan0021486 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022133625.1	9.5e-55	63.64	probable calcium-binding protein CML45 [Momordica charantia]	[more]
XP_038889669.1	4.7e-54	66.84	probable calcium-binding protein CML46 [Benincasa hispida]	[more]
KAG6578574.1	8.1e-54	66.10	putative calcium-binding protein CML46, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022993675.1	1.4e-53	68.93	probable calcium-binding protein CML30 [Cucurbita maxima]	[more]
XP_022939128.1	6.8e-53	65.54	probable calcium-binding protein CML45 [Cucurbita moschata]	[more]

BLAST of Tan0021486 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BVS4	4.6e-55	63.64	probable calcium-binding protein CML45 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1JZ70	6.7e-54	68.93	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114895...	[more]
A0A6J1FKT4	3.3e-53	65.54	probable calcium-binding protein CML45 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A1S3CAB3	6.9e-51	62.37	probable calcium-binding protein CML30 OS=Cucumis melo OX=3656 GN=LOC103498740 P...	[more]
A0A0A0KS94	8.4e-49	62.03	Calcium ion binding protein OS=Cucumis sativus OX=3659 GN=Csa_5G636620 PE=4 SV=1	[more]

BLAST of Tan0021486 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G39670.1	1.5e-29	54.03	Calcium-binding EF-hand family protein	[more]
AT3G29000.1	9.0e-27	50.82	Calcium-binding EF-hand family protein	[more]
AT4G03290.1	1.4e-16	36.64	EF hand calcium-binding protein family	[more]
AT4G12860.1	4.2e-16	36.22	EF hand calcium-binding protein family	[more]
AT1G05990.1	2.7e-15	35.66	EF hand calcium-binding protein family	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 119..147 e-value: 5.3E-4 score: 29.3 coord: 157..185 e-value: 0.0044 score: 26.2
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 120..183 e-value: 3.7E-13 score: 49.7
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 115..150 score: 13.871811
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 153..186 score: 15.099209
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 119..182 e-value: 4.0175E-15 score: 64.4913
None	No IPR available	GENE3D	1.10.238.10		coord: 57..184 e-value: 4.9E-21 score: 77.1
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 27..183
None	No IPR available	PANTHER	PTHR10891:SF745	CALCIUM-BINDING PROTEIN CML46-RELATED	coord: 27..183
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 166..178
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 128..140
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 66..184

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0021486.1	Tan0021486.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0005509	calcium ion binding