Tan0021443 (gene) Snake gourd v1

Overview
NameTan0021443
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionMBOAT (membrane bound O-acyl transferase) family protein
LocationLG01: 84633013 .. 84634320 (-)
RNA-Seq ExpressionTan0021443
SyntenyTan0021443
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTGATCTCCTCCATCTTCGCCACTCTCTCACATTAAATATGCAGACACCAAAACCTCAACAACTATGGCGCTTCACCATTCAACACTAGCACCTACCTCCGCCGCAGCCACCGTCGCCGGAAACCAAATGGACGGCGAACTCCACAACCTGGTCAAGGTATGGTCCATCGCCATTGCTCTGATTTGCTACTCTTACTATGTAGCTTCCAAAATCCCTAAGGGACTCCCAAGGTTCCTCGCTATCTTCCCCGTCCTCTTCATCTTCCTCCTCCTTCCTCTGAAACTCCACTACTTCCATTTCTGCGGTCCGACTGCCTTCTTCCTCGCTTGGCTCGGAAATTTCAAACTCCTCCTCTTCGCCTTCGATCTCGGTCCTCTGACCTCCACGCCGCTCCCGAGCCTCCTCCACTTCGCCGCCGTCCTCTGCCTTCCGATCAAGATCAAGAAGCACAAATCTCCAGAAGAATTGAAGAATCGGAGAATCGGAGTGCCGATCGCTCTCTCTTTCAAGCTCTTGCTGCTAGCGATTGTCATTCGGCTTTACGATTACCGAAATCGGATGAATCCGGTGGTGATTTTCGTTCTGTACTGCCTCCATATGTACTTCGGGATCGAAATCATTCTCGCTCTGTGTTCCCTTCCGGCGAGGGCCATCATTGGAGCTCGGATCGAACCGCAGTTCGACGAGCCGTATCTCTCCACCTCGCTTCAGGATTTCTGGAGCCGTCGATGGAATCTCATGGTTCCGAGTATTCTACGCCCGGCCGTATACGATCCCACCCGTTCCATAACTTCCCGCGTATTTGGGCCGAGGTGGGCCCAAGTTCTGGCCATGGTAGCTGCGTTTGCTGTCTCTGGCTTTATGCACGAGCTGTTGTATTATTACTTCACACGTGCCGCTCCCACGTGGGAGGTCACGTGGTTCTTTGTCCTGCACGGCGTGTGCACGGCGACGGAGGTGGCGGTGAAGGTCGCCGTGGCTGACCGGAAGGTGAGGTGGCGGCTCCCCTGGGCGGTGTCTGCGCCGATGACTCTGGCCTTTTTGATGGTGACGGCGAGGTGGTTGATGTTCCCTCAGCTAATTCGAAATGGCGTTATTGAAACGACGATTGGCGAATATTATGTCATGGCTGACTTCGTGAAGGACAAATTTGTTTAGTTGCTTTGAGTAATTTTAGAAGAAAATCTTCCTAATATAATTAAATTTATCCCAAATTATCCACTTAAATTGTTAGACTTAATTAGTGATTTAAGATTATATTAGAGTAGGAAAGACCTGAATTCGAATGCTTGTAAAATTATTTTCCC

mRNA sequence

TTGATCTCCTCCATCTTCGCCACTCTCTCACATTAAATATGCAGACACCAAAACCTCAACAACTATGGCGCTTCACCATTCAACACTAGCACCTACCTCCGCCGCAGCCACCGTCGCCGGAAACCAAATGGACGGCGAACTCCACAACCTGGTCAAGGTATGGTCCATCGCCATTGCTCTGATTTGCTACTCTTACTATGTAGCTTCCAAAATCCCTAAGGGACTCCCAAGGTTCCTCGCTATCTTCCCCGTCCTCTTCATCTTCCTCCTCCTTCCTCTGAAACTCCACTACTTCCATTTCTGCGGTCCGACTGCCTTCTTCCTCGCTTGGCTCGGAAATTTCAAACTCCTCCTCTTCGCCTTCGATCTCGGTCCTCTGACCTCCACGCCGCTCCCGAGCCTCCTCCACTTCGCCGCCGTCCTCTGCCTTCCGATCAAGATCAAGAAGCACAAATCTCCAGAAGAATTGAAGAATCGGAGAATCGGAGTGCCGATCGCTCTCTCTTTCAAGCTCTTGCTGCTAGCGATTGTCATTCGGCTTTACGATTACCGAAATCGGATGAATCCGGTGGTGATTTTCGTTCTGTACTGCCTCCATATGTACTTCGGGATCGAAATCATTCTCGCTCTGTGTTCCCTTCCGGCGAGGGCCATCATTGGAGCTCGGATCGAACCGCAGTTCGACGAGCCGTATCTCTCCACCTCGCTTCAGGATTTCTGGAGCCGTCGATGGAATCTCATGGTTCCGAGTATTCTACGCCCGGCCGTATACGATCCCACCCGTTCCATAACTTCCCGCGTATTTGGGCCGAGGTGGGCCCAAGTTCTGGCCATGGTAGCTGCGTTTGCTGTCTCTGGCTTTATGCACGAGCTGTTGTATTATTACTTCACACGTGCCGCTCCCACGTGGGAGGTCACGTGGTTCTTTGTCCTGCACGGCGTGTGCACGGCGACGGAGGTGGCGGTGAAGGTCGCCGTGGCTGACCGGAAGGTGAGGTGGCGGCTCCCCTGGGCGGTGTCTGCGCCGATGACTCTGGCCTTTTTGATGGTGACGGCGAGGTGGTTGATGTTCCCTCAGCTAATTCGAAATGGCGTTATTGAAACGACGATTGGCGAATATTATGTCATGGCTGACTTCGTGAAGGACAAATTTGTTTAGTTGCTTTGAGTAATTTTAGAAGAAAATCTTCCTAATATAATTAAATTTATCCCAAATTATCCACTTAAATTGTTAGACTTAATTAGTGATTTAAGATTATATTAGAGTAGGAAAGACCTGAATTCGAATGCTTGTAAAATTATTTTCCC

Coding sequence (CDS)

ATGGCGCTTCACCATTCAACACTAGCACCTACCTCCGCCGCAGCCACCGTCGCCGGAAACCAAATGGACGGCGAACTCCACAACCTGGTCAAGGTATGGTCCATCGCCATTGCTCTGATTTGCTACTCTTACTATGTAGCTTCCAAAATCCCTAAGGGACTCCCAAGGTTCCTCGCTATCTTCCCCGTCCTCTTCATCTTCCTCCTCCTTCCTCTGAAACTCCACTACTTCCATTTCTGCGGTCCGACTGCCTTCTTCCTCGCTTGGCTCGGAAATTTCAAACTCCTCCTCTTCGCCTTCGATCTCGGTCCTCTGACCTCCACGCCGCTCCCGAGCCTCCTCCACTTCGCCGCCGTCCTCTGCCTTCCGATCAAGATCAAGAAGCACAAATCTCCAGAAGAATTGAAGAATCGGAGAATCGGAGTGCCGATCGCTCTCTCTTTCAAGCTCTTGCTGCTAGCGATTGTCATTCGGCTTTACGATTACCGAAATCGGATGAATCCGGTGGTGATTTTCGTTCTGTACTGCCTCCATATGTACTTCGGGATCGAAATCATTCTCGCTCTGTGTTCCCTTCCGGCGAGGGCCATCATTGGAGCTCGGATCGAACCGCAGTTCGACGAGCCGTATCTCTCCACCTCGCTTCAGGATTTCTGGAGCCGTCGATGGAATCTCATGGTTCCGAGTATTCTACGCCCGGCCGTATACGATCCCACCCGTTCCATAACTTCCCGCGTATTTGGGCCGAGGTGGGCCCAAGTTCTGGCCATGGTAGCTGCGTTTGCTGTCTCTGGCTTTATGCACGAGCTGTTGTATTATTACTTCACACGTGCCGCTCCCACGTGGGAGGTCACGTGGTTCTTTGTCCTGCACGGCGTGTGCACGGCGACGGAGGTGGCGGTGAAGGTCGCCGTGGCTGACCGGAAGGTGAGGTGGCGGCTCCCCTGGGCGGTGTCTGCGCCGATGACTCTGGCCTTTTTGATGGTGACGGCGAGGTGGTTGATGTTCCCTCAGCTAATTCGAAATGGCGTTATTGAAACGACGATTGGCGAATATTATGTCATGGCTGACTTCGTGAAGGACAAATTTGTTTAG

Protein sequence

MALHHSTLAPTSAAATVAGNQMDGELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLAIFPVLFIFLLLPLKLHYFHFCGPTAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAVLCLPIKIKKHKSPEELKNRRIGVPIALSFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHMYFGIEIILALCSLPARAIIGARIEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSITSRVFGPRWAQVLAMVAAFAVSGFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEVAVKVAVADRKVRWRLPWAVSAPMTLAFLMVTARWLMFPQLIRNGVIETTIGEYYVMADFVKDKFV
Homology
BLAST of Tan0021443 vs. ExPASy Swiss-Prot
Match: Q9XGY6 (Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.3e-79
Identity = 171/355 (48.17%), Postives = 219/355 (61.69%), Query Frame = 0

Query: 21  QMDGELHNLVKVWSIAIALICYSYYVASKIPK-GLPRFLAIFPVLFIFLLLPLKLHYFHF 80
           +++ EL    +VW  AIA  CY  +V +  P  G  R L + PV+ +F+ LPL+L  FH 
Sbjct: 2   EVEKELKTFSEVWISAIAAACYCRFVPAVAPHGGALRLLLLLPVVLLFIFLPLRLSSFHL 61

Query: 81  CGPTAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAVLCLPIKIKKHKSPEELKNRR 140
            GPTA +L WL NFKLLLFAF LGPL S P  SLLHF +   LPIK +   S +  KN+R
Sbjct: 62  GGPTALYLVWLANFKLLLFAFHLGPL-SNPSLSLLHFISTTLLPIKFRDDPSNDHEKNKR 121

Query: 141 IGVPIALSF----------KLLLLAIVIRLYDYRNRMNPVVIFVLYCLHMYFGIEIILAL 200
                 LSF          KL+  A ++++Y++R  +   VI VLYC H Y G EI LA 
Sbjct: 122 -----TLSFEWRKVVLFVAKLVFFAGILKIYEFRKDLPHFVISVLYCFHFYLGTEITLAA 181

Query: 201 CSLPARAIIGARIEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSITSRVFGP 260
            ++ ARA +G  + PQF+EPYL+TSLQDFW RRWNLMV  IL    Y P R + SR    
Sbjct: 182 SAVIARATLGLDLYPQFNEPYLATSLQDFWGRRWNLMVSDILGLTTYQPVRRVLSRWVRL 241

Query: 261 RWAQVLAMVAAFAVSGFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEVAVKVAVADRK 320
           RW    AM+ AF VSG MHE+ ++Y TRA P+WEVT FFVLHGVCTA E+ VK AV+ + 
Sbjct: 242 RWEVAGAMLVAFTVSGLMHEVFFFYLTRARPSWEVTGFFVLHGVCTAVEMVVKKAVSGKV 301

Query: 321 VRWRLPWAVSAPMTLAFLMVTARWLMFPQLIRNGVIETTIGEYYVMADFVKDKFV 365
              RL   VS  +T+ F+MVT  WL  PQL+R+GV   TI EY VM ++ + K +
Sbjct: 302 ---RLRREVSGALTVGFVMVTGGWLFLPQLVRHGVDLKTIDEYPVMFNYTQKKLM 347

BLAST of Tan0021443 vs. ExPASy Swiss-Prot
Match: Q9FJ76 (Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=AT5 PE=2 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.1e-78
Identity = 161/340 (47.35%), Postives = 212/340 (62.35%), Query Frame = 0

Query: 22  MDGELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLAIFPVLFIFLLLPLKLHYFHFCG 81
           MD EL NL+KVW  AI  I Y YY+  +I  G PRFL++ PVL +FL+LPL     H   
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 82  PTAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAVLCLPIKIKKH-KSPEELKNRRI 141
            TAFFL WL NFKL+LF+FD GPL   P  +   F    C PIK++++ KS   L     
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPIP-TNFPRFFCFTCFPIKVQQNPKSQNHLPK--- 120

Query: 142 GVPIALSFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHMYFGIEIILALCSLPARAIIGA 201
              +  + KL + A+++ LY YR  ++P ++  LY +H+Y  IEIIL    +     +G 
Sbjct: 121 ---LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLGC 180

Query: 202 RIEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSITSRVFGPRWAQVLAMVAA 261
            +EPQ ++PYL+TSLQDFW RRWNLMVP+ILRPAVY P R ++ R     WA    ++AA
Sbjct: 181 DLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAA 240

Query: 262 FAVSGFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEVAVKVAVADRKVRWRLPWAVSA 321
           F VSG +HELL++Y  R  PT EVT FFVLHGVCTA E+AVK      + RWRL   VS 
Sbjct: 241 FIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQ-RWRLSPGVSR 300

Query: 322 PMTLAFLMVTARWLMFPQLIRNGVIETTIGEYYVMADFVK 361
            +T+ F+ VT  WL  PQL R+GV+E    E  ++ +F+K
Sbjct: 301 VLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIK 332

BLAST of Tan0021443 vs. ExPASy Swiss-Prot
Match: Q9FJ75 (Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=AT4 PE=2 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.5e-78
Identity = 158/342 (46.20%), Postives = 215/342 (62.87%), Query Frame = 0

Query: 22  MDGELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLAIFPVLFIFLLLPLKLHYFHFCG 81
           M+ EL +L+KVW  AI  I Y YY +++I  G+ R L++ PV  +FL+LPL +   HF G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 82  PTAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAVLCLPIKIKKHKSPEELKNRRIG 141
            TAFFL+WL NFKL+LF+FD GPL   P  +L  F    C PIK++++  P+    +   
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVP-SNLSRFVCFTCFPIKLQQNPKPQNQMPK--- 120

Query: 142 VPIALSFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHMYFGIEIILALCSLPARAIIGAR 201
                + KL    +++ +Y+Y+  M+P V+ VLY LH+Y   EI+LA   +     +   
Sbjct: 121 --WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCD 180

Query: 202 IEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSITSRVFGPRWAQVLAMVAAF 261
           +EP F+EPYLSTSLQDFW RRWNLMVP+ILRPAVY P R +  R      A  L + A+F
Sbjct: 181 LEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASF 240

Query: 262 AVSGFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEVAVKVAVADRKVRWRLPWAVSAP 321
            VSG +HEL+++YFTR +PT EVT FFVLHGVCTA E A K     R  RW++   VS  
Sbjct: 241 LVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVR--RWKVSQMVSRL 300

Query: 322 MTLAFLMVTARWLMFPQLIRNGVIETTIGEYYVMADFVKDKF 364
           +T+ F+++T  WL FP L R+G+IE    E ++   FVK KF
Sbjct: 301 LTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKF 334

BLAST of Tan0021443 vs. ExPASy Swiss-Prot
Match: Q9FJ72 (Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=AT1 PE=2 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 1.9e-78
Identity = 158/344 (45.93%), Postives = 214/344 (62.21%), Query Frame = 0

Query: 22  MDGELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLAIFPVLFIFLLLPLKLHYFHFCG 81
           M+ +  NL++VW  A+  + Y YY++SK+ KG+ R L+I PV  +FL+LPL L   HFC 
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 82  PTAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAVLCLPIKIKKHKSPEELKNRRIG 141
            +  FL+WL NFKLLLFAFD GPL   P P L  F    CLPIKI++  SP  + N    
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLP-PKLSRFICFACLPIKIRQDPSPNAIPNLH-P 120

Query: 142 VPI---ALSFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHMYFGIEIILALCSLPARAII 201
            P+    L+ K+L+L +++ +Y+YR+ +   V+  LYCLH+Y  +E++L         ++
Sbjct: 121 KPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLL 180

Query: 202 GARIEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSITSRVFGPRWAQVLAMV 261
           G  IEP F+EPYL+TSLQDFWSRRWNLMV ++LR  V+ P +    R+  P  A    ++
Sbjct: 181 GCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVM 240

Query: 262 AAFAVSGFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEVAVKVAVADRKVRWRLP--W 321
           A+F VSG MHELLY+Y  R  PTWEVT FFVLHG  TATE+AVK      + +W  P   
Sbjct: 241 ASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVK------RTQWLRPPHR 300

Query: 322 AVSAPMTLAFLMVTARWLMFPQLIRNGVIETTIGEYYVMADFVK 361
           AVS  + L F+ VT  WL   Q++RN V E  IGE  ++ D  K
Sbjct: 301 AVSGLVVLTFVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAK 336

BLAST of Tan0021443 vs. ExPASy Swiss-Prot
Match: Q9SV07 (Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE=1 SV=1)

HSP 1 Score: 293.1 bits (749), Expect = 4.3e-78
Identity = 145/334 (43.41%), Postives = 210/334 (62.87%), Query Frame = 0

Query: 28  NLVKVWSIAIALICYSYYVASKIPKGLPRFLAIFPVLFIFLLLPLKLHYFHFCGPTAFFL 87
           + +K W + I  +CY++++A  +PKG+ R +  FPV  IF ++P  ++  H  G TAFF+
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 88  AWLGNFKLLLFAFDLGPLTSTPLP-SLLHFAAVLCLPIKIKKHKSPEELKNRRIGV--PI 147
           AWL NFKLLLFA   GPL+S   P SL  F AV CLPIKI+    P +  +       P+
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPL 122

Query: 148 ALSFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHMYFGIEIILALCSLPARAIIGARIEP 207
             + K + + ++I+ Y+Y  ++   V+  LY +H+YF +EIILA  +   RA+    +EP
Sbjct: 123 IYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEP 182

Query: 208 QFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSITSRVFGPRWAQVLAMVAAFAVS 267
           QF++PYL+TSLQDFW RRWNLMV  ILRP VY+P+  + S V GP ++Q+LA    F VS
Sbjct: 183 QFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFGTFVVS 242

Query: 268 GFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEVAVKVAVADRKVRWRLPWAVSAPMTL 327
           G MHEL+++Y  R  P W++ WFF+++G CT  E+A+K  +     RWR P A+S  +TL
Sbjct: 243 GIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTI---NGRWRFPKAISQVLTL 302

Query: 328 AFLMVTARWLMFPQLIRNGVIETTIGEYYVMADF 359
            F+MVTA WL  P+  R  ++E  + EY  +  F
Sbjct: 303 TFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAF 332

BLAST of Tan0021443 vs. NCBI nr
Match: KAG7037354.1 (putative long-chain-alcohol O-fatty-acyltransferase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 592.4 bits (1526), Expect = 2.6e-165
Identity = 294/364 (80.77%), Postives = 323/364 (88.74%), Query Frame = 0

Query: 1   MALHHSTLAPTSAAATVAGNQMDGELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLAI 60
           MA  HSTL PTSA    AGN MDGEL +L++VWSIAIAL+CYSYYVASKIPKGLPR L++
Sbjct: 1   MADGHSTLPPTSA----AGNLMDGELSSLLQVWSIAIALMCYSYYVASKIPKGLPRLLSL 60

Query: 61  FPVLFIFLLLPLKLHYFHFCGPTAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAVL 120
           FPVLF+FLLLPL LH FHF GPTAFFLAWLGNFKLLLFAFDLGPLTS PLPSLLHFAAVL
Sbjct: 61  FPVLFVFLLLPLNLHSFHFGGPTAFFLAWLGNFKLLLFAFDLGPLTSLPLPSLLHFAAVL 120

Query: 121 CLPIKIKKHKSPEELKNRRIGVPIALSFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHMY 180
           CLPIKIKK K PEE KNRRI  PI LS K+LLLAI+IRLY YRNR+NP +IFVLYCLHMY
Sbjct: 121 CLPIKIKKQKHPEESKNRRIVEPIVLSVKVLLLAIIIRLYSYRNRLNPAMIFVLYCLHMY 180

Query: 181 FGIEIILALCSLPARAIIGARIEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTR 240
            GIEI+LALCSLPARA+IGA IEPQF++PYLSTSLQDFWSRRWNLMVPSILRPAVYDPTR
Sbjct: 181 IGIEIVLALCSLPARAMIGAPIEPQFNKPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTR 240

Query: 241 SITSRVFGPRWAQVLAMVAAFAVSGFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEVA 300
           S+TSR+F P+WAQ LAMV  F VSGFMHEL+YYYFTR APTWEVTWFFVLHGVCTA E+A
Sbjct: 241 SVTSRIFRPKWAQFLAMVVTFTVSGFMHELMYYYFTRVAPTWEVTWFFVLHGVCTAAELA 300

Query: 301 VKVAVADRKVRWRLPWAVSAPMTLAFLMVTARWLMFPQLIRNGVIETTIGEYYVMADFVK 360
            K AVA RK+RWR+P  VS PMT+ FL+ TARWLMFPQLIRNGV+E+TIGEYYV+ +F+K
Sbjct: 301 AKAAVAGRKMRWRVPRVVSGPMTVGFLIATARWLMFPQLIRNGVVESTIGEYYVLVNFMK 360

Query: 361 DKFV 365
           DKFV
Sbjct: 361 DKFV 360

BLAST of Tan0021443 vs. NCBI nr
Match: XP_023525242.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 588.2 bits (1515), Expect = 4.9e-164
Identity = 297/364 (81.59%), Postives = 322/364 (88.46%), Query Frame = 0

Query: 1   MALHHSTLAPTSAAATVAGNQMDGELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLAI 60
           MA  HSTL PTSA    AGN MDGEL +L++VWSIAIALI YSYYVASKIPKGLPR L++
Sbjct: 1   MADGHSTLPPTSA----AGNLMDGELSSLLQVWSIAIALIFYSYYVASKIPKGLPRLLSL 60

Query: 61  FPVLFIFLLLPLKLHYFHFCGPTAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAVL 120
           FPVLFIFLLLPL LH FHF GPTAFFLAWLGNFKLLLFAFDLGPLTS PLPSLLHFAAVL
Sbjct: 61  FPVLFIFLLLPLNLHSFHFGGPTAFFLAWLGNFKLLLFAFDLGPLTSLPLPSLLHFAAVL 120

Query: 121 CLPIKIKKHKSPEELKNRRIGVPIALSFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHMY 180
           CLPIKIKK K PEE KNRRI  PI LS K+LLLAI+IRLY YRNRMNP +IFVLYCLHMY
Sbjct: 121 CLPIKIKKQKHPEESKNRRIVEPIVLSVKVLLLAIIIRLYSYRNRMNPAMIFVLYCLHMY 180

Query: 181 FGIEIILALCSLPARAIIGARIEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTR 240
            GIEI+LALCSLPARA+IGA IEPQF++PYLSTSLQDFWSRRWNLMVPSILRPAVYDPTR
Sbjct: 181 IGIEIVLALCSLPARAMIGAPIEPQFNKPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTR 240

Query: 241 SITSRVFGPRWAQVLAMVAAFAVSGFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEVA 300
           SITSR+F P+WAQ LAMV  FAVSGFMHEL+YYYFTR APTWEVTWFFVLHGV TA E+A
Sbjct: 241 SITSRIFRPKWAQFLAMVVTFAVSGFMHELMYYYFTRVAPTWEVTWFFVLHGVFTAAELA 300

Query: 301 VKVAVADRKVRWRLPWAVSAPMTLAFLMVTARWLMFPQLIRNGVIETTIGEYYVMADFVK 360
            K AVA RK+RWR+P  VS PMT+ FL+ TARWLMFPQLIRNGV+E+TIGEYYV+ +F+K
Sbjct: 301 AKAAVAARKMRWRVPRVVSGPMTVGFLIATARWLMFPQLIRNGVVESTIGEYYVLVNFMK 360

Query: 361 DKFV 365
           DKFV
Sbjct: 361 DKFV 360

BLAST of Tan0021443 vs. NCBI nr
Match: XP_022981522.1 (probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita maxima])

HSP 1 Score: 585.1 bits (1507), Expect = 4.2e-163
Identity = 292/364 (80.22%), Postives = 319/364 (87.64%), Query Frame = 0

Query: 1   MALHHSTLAPTSAAATVAGNQMDGELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLAI 60
           MA  HSTL PTSA    AGN MDGEL +L++VWSIAIALICYSYYVASKI KGLPR L++
Sbjct: 1   MADGHSTLPPTSA----AGNLMDGELSSLLQVWSIAIALICYSYYVASKIAKGLPRLLSL 60

Query: 61  FPVLFIFLLLPLKLHYFHFCGPTAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAVL 120
           FPVLFIFLLLPL LH FHF GPTAFFLAWLGNFKLLLFAFDLGPLT  PLPSLLHFAAVL
Sbjct: 61  FPVLFIFLLLPLNLHSFHFGGPTAFFLAWLGNFKLLLFAFDLGPLTYLPLPSLLHFAAVL 120

Query: 121 CLPIKIKKHKSPEELKNRRIGVPIALSFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHMY 180
           CLPIKIKK K PEE KNRRI  PI LS K+LLLAI+IRLY YRNRMNP +IFVLYCLHMY
Sbjct: 121 CLPIKIKKQKHPEESKNRRIVEPIVLSVKILLLAIIIRLYSYRNRMNPAMIFVLYCLHMY 180

Query: 181 FGIEIILALCSLPARAIIGARIEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTR 240
            GIEI+LALCSLP+RA+IG  IEPQF++PYLSTSLQDFWSRRWNLMVPSILRPAVYDPTR
Sbjct: 181 IGIEIVLALCSLPSRAMIGEPIEPQFNKPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTR 240

Query: 241 SITSRVFGPRWAQVLAMVAAFAVSGFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEVA 300
           S+TSR+F P+WAQ LAMV  FAVSG MHEL+YYYFTR APTWEVTWFFVLHGVCTA EVA
Sbjct: 241 SLTSRIFRPKWAQFLAMVVTFAVSGLMHELMYYYFTRVAPTWEVTWFFVLHGVCTAAEVA 300

Query: 301 VKVAVADRKVRWRLPWAVSAPMTLAFLMVTARWLMFPQLIRNGVIETTIGEYYVMADFVK 360
            K AV  RK+RWR+P  +S PMT+ FL+ TARWLMFPQLIRNGV+E+TIGEYYV+ +F+K
Sbjct: 301 AKAAVTGRKIRWRVPRVLSGPMTVGFLIATARWLMFPQLIRNGVVESTIGEYYVLVNFMK 360

Query: 361 DKFV 365
           DKFV
Sbjct: 361 DKFV 360

BLAST of Tan0021443 vs. NCBI nr
Match: XP_038898664.1 (probable long-chain-alcohol O-fatty-acyltransferase 4 [Benincasa hispida])

HSP 1 Score: 565.1 bits (1455), Expect = 4.5e-157
Identity = 286/365 (78.36%), Postives = 309/365 (84.66%), Query Frame = 0

Query: 1   MALHHSTLAPTSAAATVAGNQMD-GELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLA 60
           MA+HHSTLAP SAA T AG QMD GE H L KVW+IAIALI YSYY+ASKI KGLPR +A
Sbjct: 1   MAVHHSTLAPNSAAPTAAGIQMDGGEFHRLAKVWTIAIALISYSYYIASKIHKGLPRLIA 60

Query: 61  IFPVLFIFLLLPLKLHYFHFCGPTAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAV 120
           +FPV+FIFLLLPL LH FH CGPTAF LAWLGNFKLLLFAFDLGPL S+PLPSLLHFA +
Sbjct: 61  LFPVIFIFLLLPLNLHSFHLCGPTAFLLAWLGNFKLLLFAFDLGPLFSSPLPSLLHFATM 120

Query: 121 LCLPIKIKKHKSPEELKNRRIGVPIALSFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHM 180
           LCLPIKI   KS    + RR G  I LSFK+LLLAIVIRLYDYRNRM+P +  VLYCLH+
Sbjct: 121 LCLPIKINNQKS----EIRRTGNQIVLSFKVLLLAIVIRLYDYRNRMDPAIASVLYCLHL 180

Query: 181 YFGIEIILALCSLPARAIIGARIEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPT 240
           YFGIEI+LALCSLPA+AI GA IEPQFDEPYLSTSLQDFW  RWNLMVPSILRPAVY+PT
Sbjct: 181 YFGIEIVLALCSLPAKAIFGAPIEPQFDEPYLSTSLQDFWGHRWNLMVPSILRPAVYNPT 240

Query: 241 RSITSRVFGPRWAQVLAMVAAFAVSGFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEV 300
           R ITSRVFGPRWAQ LAMVA FAVSGFMHEL++YYFTRAAP WEVTWFFVLHG CTA EV
Sbjct: 241 RFITSRVFGPRWAQFLAMVATFAVSGFMHELIFYYFTRAAPMWEVTWFFVLHGFCTAAEV 300

Query: 301 AVKVAVADRKVRWRLPWAVSAPMTLAFLMVTARWLMFPQLIRNGVIETTIGEYYVMADFV 360
           A K AVA RKVRW++   VS PMTL FLM TARWLMFPQLIRNGV++ TIGEYY MA FV
Sbjct: 301 AAKAAVASRKVRWKVHRMVSGPMTLVFLMATARWLMFPQLIRNGVVDRTIGEYYTMAHFV 360

Query: 361 KDKFV 365
           K K +
Sbjct: 361 KGKLI 361

BLAST of Tan0021443 vs. NCBI nr
Match: XP_008448089.1 (PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo])

HSP 1 Score: 508.8 bits (1309), Expect = 3.8e-140
Identity = 262/365 (71.78%), Postives = 290/365 (79.45%), Query Frame = 0

Query: 1   MALHHSTLAPTSAAATVAGNQM-DGELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLA 60
           M ++HSTL P S     AGNQM DGE + L +VW+IAI LI YSYY ASKIPKG+PR LA
Sbjct: 1   MTVYHSTLPPNS-----AGNQMNDGEFYRLAEVWTIAIPLIAYSYYFASKIPKGIPRLLA 60

Query: 61  IFPVLFIFLLLPLKLHYFHFCGPTAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAV 120
           +FPVLFIFLLLPL L  FH CGPTAFFL+WLGNFKLLLFAFDLGPL S+PLPS LHFAA 
Sbjct: 61  LFPVLFIFLLLPLNLRSFHLCGPTAFFLSWLGNFKLLLFAFDLGPLFSSPLPSFLHFAAT 120

Query: 121 LCLPIKIKKHKSPEELKNRRIGVPIALSFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHM 180
           LCLPIKI  HKS    + RR G PI L  K+LLLAIV+RLYDYRNRM+P ++ VLY LH+
Sbjct: 121 LCLPIKISSHKS----EIRRNGNPIVLCLKVLLLAIVVRLYDYRNRMDPAIVSVLYFLHL 180

Query: 181 YFGIEIILALCSLPARAIIGARIEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPT 240
           YFGIEI+LA+CSLP +AI GA  EPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPT
Sbjct: 181 YFGIEIVLAVCSLPGKAIFGAPFEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPT 240

Query: 241 RSITSRVFGPRWAQVLAMVAAFAVSGFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEV 300
           R ITSR FG RWAQ L MVA F VSG MHEL++YYFTRA PTWEVT FFVLHG C A EV
Sbjct: 241 RIITSRAFGRRWAQFLGMVATFVVSGLMHELIFYYFTRAPPTWEVTCFFVLHGFCMAAEV 300

Query: 301 AVKVAVADRKVRWRLPWAVSAPMTLAFLMVTARWLMFPQLIRNGVIETTIGEYYVMADFV 360
           A K AVA     W +   VS P++L FLM TARWLM PQLIRNGV++  IGEYY MA F+
Sbjct: 301 AAKAAVAG---LWNIHPMVSGPISLVFLMATARWLMLPQLIRNGVVDKAIGEYYAMAHFL 353

Query: 361 KDKFV 365
           K KF+
Sbjct: 361 KGKFI 353

BLAST of Tan0021443 vs. ExPASy TrEMBL
Match: A0A6J1IU70 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=3661 GN=LOC111480612 PE=3 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 2.0e-163
Identity = 292/364 (80.22%), Postives = 319/364 (87.64%), Query Frame = 0

Query: 1   MALHHSTLAPTSAAATVAGNQMDGELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLAI 60
           MA  HSTL PTSA    AGN MDGEL +L++VWSIAIALICYSYYVASKI KGLPR L++
Sbjct: 1   MADGHSTLPPTSA----AGNLMDGELSSLLQVWSIAIALICYSYYVASKIAKGLPRLLSL 60

Query: 61  FPVLFIFLLLPLKLHYFHFCGPTAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAVL 120
           FPVLFIFLLLPL LH FHF GPTAFFLAWLGNFKLLLFAFDLGPLT  PLPSLLHFAAVL
Sbjct: 61  FPVLFIFLLLPLNLHSFHFGGPTAFFLAWLGNFKLLLFAFDLGPLTYLPLPSLLHFAAVL 120

Query: 121 CLPIKIKKHKSPEELKNRRIGVPIALSFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHMY 180
           CLPIKIKK K PEE KNRRI  PI LS K+LLLAI+IRLY YRNRMNP +IFVLYCLHMY
Sbjct: 121 CLPIKIKKQKHPEESKNRRIVEPIVLSVKILLLAIIIRLYSYRNRMNPAMIFVLYCLHMY 180

Query: 181 FGIEIILALCSLPARAIIGARIEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTR 240
            GIEI+LALCSLP+RA+IG  IEPQF++PYLSTSLQDFWSRRWNLMVPSILRPAVYDPTR
Sbjct: 181 IGIEIVLALCSLPSRAMIGEPIEPQFNKPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTR 240

Query: 241 SITSRVFGPRWAQVLAMVAAFAVSGFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEVA 300
           S+TSR+F P+WAQ LAMV  FAVSG MHEL+YYYFTR APTWEVTWFFVLHGVCTA EVA
Sbjct: 241 SLTSRIFRPKWAQFLAMVVTFAVSGLMHELMYYYFTRVAPTWEVTWFFVLHGVCTAAEVA 300

Query: 301 VKVAVADRKVRWRLPWAVSAPMTLAFLMVTARWLMFPQLIRNGVIETTIGEYYVMADFVK 360
            K AV  RK+RWR+P  +S PMT+ FL+ TARWLMFPQLIRNGV+E+TIGEYYV+ +F+K
Sbjct: 301 AKAAVTGRKIRWRVPRVLSGPMTVGFLIATARWLMFPQLIRNGVVESTIGEYYVLVNFMK 360

Query: 361 DKFV 365
           DKFV
Sbjct: 361 DKFV 360

BLAST of Tan0021443 vs. ExPASy TrEMBL
Match: A0A1S3BIX3 (probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo OX=3656 GN=LOC103490376 PE=3 SV=1)

HSP 1 Score: 508.8 bits (1309), Expect = 1.8e-140
Identity = 262/365 (71.78%), Postives = 290/365 (79.45%), Query Frame = 0

Query: 1   MALHHSTLAPTSAAATVAGNQM-DGELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLA 60
           M ++HSTL P S     AGNQM DGE + L +VW+IAI LI YSYY ASKIPKG+PR LA
Sbjct: 1   MTVYHSTLPPNS-----AGNQMNDGEFYRLAEVWTIAIPLIAYSYYFASKIPKGIPRLLA 60

Query: 61  IFPVLFIFLLLPLKLHYFHFCGPTAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAV 120
           +FPVLFIFLLLPL L  FH CGPTAFFL+WLGNFKLLLFAFDLGPL S+PLPS LHFAA 
Sbjct: 61  LFPVLFIFLLLPLNLRSFHLCGPTAFFLSWLGNFKLLLFAFDLGPLFSSPLPSFLHFAAT 120

Query: 121 LCLPIKIKKHKSPEELKNRRIGVPIALSFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHM 180
           LCLPIKI  HKS    + RR G PI L  K+LLLAIV+RLYDYRNRM+P ++ VLY LH+
Sbjct: 121 LCLPIKISSHKS----EIRRNGNPIVLCLKVLLLAIVVRLYDYRNRMDPAIVSVLYFLHL 180

Query: 181 YFGIEIILALCSLPARAIIGARIEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPT 240
           YFGIEI+LA+CSLP +AI GA  EPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPT
Sbjct: 181 YFGIEIVLAVCSLPGKAIFGAPFEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPT 240

Query: 241 RSITSRVFGPRWAQVLAMVAAFAVSGFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEV 300
           R ITSR FG RWAQ L MVA F VSG MHEL++YYFTRA PTWEVT FFVLHG C A EV
Sbjct: 241 RIITSRAFGRRWAQFLGMVATFVVSGLMHELIFYYFTRAPPTWEVTCFFVLHGFCMAAEV 300

Query: 301 AVKVAVADRKVRWRLPWAVSAPMTLAFLMVTARWLMFPQLIRNGVIETTIGEYYVMADFV 360
           A K AVA     W +   VS P++L FLM TARWLM PQLIRNGV++  IGEYY MA F+
Sbjct: 301 AAKAAVAG---LWNIHPMVSGPISLVFLMATARWLMLPQLIRNGVVDKAIGEYYAMAHFL 353

Query: 361 KDKFV 365
           K KF+
Sbjct: 361 KGKFI 353

BLAST of Tan0021443 vs. ExPASy TrEMBL
Match: A0A5A7TX12 (Putative long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G002780 PE=3 SV=1)

HSP 1 Score: 495.0 bits (1273), Expect = 2.7e-136
Identity = 250/342 (73.10%), Postives = 276/342 (80.70%), Query Frame = 0

Query: 23  DGELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLAIFPVLFIFLLLPLKLHYFHFCGP 82
           DGE + L +VW+IAI LI YSYY ASKIPKG+PR LA+FPVLFIFLLLPL L  FH CGP
Sbjct: 3   DGEFYRLAEVWTIAIPLIAYSYYFASKIPKGIPRLLALFPVLFIFLLLPLNLRSFHLCGP 62

Query: 83  TAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAVLCLPIKIKKHKSPEELKNRRIGV 142
           TAFFL+WLGNFKLLLFAFDLGPL S+PLPS LHFAA LCLPIKI  HKS    + RR G 
Sbjct: 63  TAFFLSWLGNFKLLLFAFDLGPLFSSPLPSFLHFAATLCLPIKISSHKS----EIRRNGN 122

Query: 143 PIALSFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHMYFGIEIILALCSLPARAIIGARI 202
           PI L  K+LLLAIV+RLYDYRNRM+P ++ VLY LH+YFGIEI+LA+CSLP +AI GA  
Sbjct: 123 PIVLCLKVLLLAIVVRLYDYRNRMDPAIVSVLYFLHLYFGIEIVLAVCSLPGKAIFGAPF 182

Query: 203 EPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSITSRVFGPRWAQVLAMVAAFA 262
           EPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTR ITSR FG RWAQ L MVA F 
Sbjct: 183 EPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRIITSRAFGRRWAQFLGMVATFV 242

Query: 263 VSGFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEVAVKVAVADRKVRWRLPWAVSAPM 322
           VSG MHEL++YYFTRA PTWEVT FFVLHG C A EVA K AVA     W +   VS P+
Sbjct: 243 VSGLMHELIFYYFTRAPPTWEVTCFFVLHGFCMAAEVAAKAAVAG---LWNIHPMVSGPI 302

Query: 323 TLAFLMVTARWLMFPQLIRNGVIETTIGEYYVMADFVKDKFV 365
           +L FLM TARWLM PQLIRNGV++  IGEYY MA F+K KF+
Sbjct: 303 SLVFLMATARWLMLPQLIRNGVVDKAIGEYYAMAHFLKGKFI 337

BLAST of Tan0021443 vs. ExPASy TrEMBL
Match: A0A6J5X6S6 (MBOAT_2 domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCUS28007 PE=3 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 5.8e-110
Identity = 205/373 (54.96%), Postives = 257/373 (68.90%), Query Frame = 0

Query: 22  MDGELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLAIFPVLFIFLLLPLKLHYFHFCG 81
           MDGE++N +KVW +    +CY YY+A+KIPKG+ R +++ P+ +IF++LPL LH FH CG
Sbjct: 1   MDGEINNFIKVWILTNTSLCYCYYIAAKIPKGIIRLISLLPIFYIFIILPLNLHSFHLCG 60

Query: 82  PTAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAVLCLPIKIKKH---KSPEELKNR 141
           PT FFL WLG FKLLLF+F+LGPL+ TP P+L+ F ++ CLPIKIK++   KSP+     
Sbjct: 61  PTTFFLVWLGIFKLLLFSFNLGPLSPTP-PTLVQFISIACLPIKIKQNPPQKSPQNTDQN 120

Query: 142 RI----------------------------GVP---IALSFKLLLLAIVIRLYDYRNRMN 201
            +                             VP   I L+ K LLLA++IR Y+YR  ++
Sbjct: 121 PLTPQNNKNPSHQTTPKPKKSVNPIDQTLTNVPNKSILLAIKALLLALIIRTYEYRPHLH 180

Query: 202 PVVIFVLYCLHMYFGIEIILALCSLPARAIIGARIEPQFDEPYLSTSLQDFWSRRWNLMV 261
           P +I  LYC HMY GIE++LAL ++PARAI+G  +EPQF EPYLSTSLQDFW RRWNLMV
Sbjct: 181 PYIILALYCCHMYLGIELVLALSAVPARAILGFELEPQFTEPYLSTSLQDFWGRRWNLMV 240

Query: 262 PSILRPAVYDPTRSITSRVFGPRWAQVLAMVAAFAVSGFMHELLYYYFTRAAPTWEVTWF 321
            +ILRP  YDP R I+ R+ GPRWA+ LA+++ FAVSG MHE +YYY TR +PTWEVTWF
Sbjct: 241 TNILRPVAYDPVRRISMRILGPRWARFLAVMSTFAVSGLMHEAIYYYLTRVSPTWEVTWF 300

Query: 322 FVLHGVCTATEVAVKVAVADRKVRWRLPWAVSAPMTLAFLMVTARWLMFPQLIRNGVIET 361
           FVLHGVC A EV VK A AD   RWRL   VS P+T+ FL VT  WL FPQL+RNGV   
Sbjct: 301 FVLHGVCVAVEVEVKKAAAD---RWRLHPVVSGPLTIVFLAVTGNWLFFPQLLRNGVDLK 360

BLAST of Tan0021443 vs. ExPASy TrEMBL
Match: A0A6J5UNH8 (MBOAT_2 domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS28346 PE=3 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 5.8e-110
Identity = 205/373 (54.96%), Postives = 257/373 (68.90%), Query Frame = 0

Query: 22  MDGELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLAIFPVLFIFLLLPLKLHYFHFCG 81
           MDGE++N +KVW +    +CY YY+A+KIPKG+ R +++ P+ +IF++LPL LH FH CG
Sbjct: 1   MDGEINNFIKVWILTNTSLCYCYYIAAKIPKGIIRLISLLPIFYIFIILPLNLHSFHLCG 60

Query: 82  PTAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAVLCLPIKIKKH---KSPEELKNR 141
           PT FFL WLG FKLLLF+F+LGPL+ TP P+L+ F ++ CLPIKIK++   KSP+     
Sbjct: 61  PTTFFLVWLGIFKLLLFSFNLGPLSPTP-PTLVQFISIACLPIKIKQNPPQKSPQNTDQN 120

Query: 142 RI----------------------------GVP---IALSFKLLLLAIVIRLYDYRNRMN 201
            +                             VP   I L+ K LLLA++IR Y+YR  ++
Sbjct: 121 PLTPQNNKNPSHQTTPKPKKSVNPIDQTLTNVPNKSILLAIKALLLALIIRTYEYRPHLH 180

Query: 202 PVVIFVLYCLHMYFGIEIILALCSLPARAIIGARIEPQFDEPYLSTSLQDFWSRRWNLMV 261
           P +I  LYC HMY GIE++LAL ++PARAI+G  +EPQF EPYLSTSLQDFW RRWNLMV
Sbjct: 181 PYIILALYCCHMYLGIELVLALSAVPARAILGFELEPQFTEPYLSTSLQDFWGRRWNLMV 240

Query: 262 PSILRPAVYDPTRSITSRVFGPRWAQVLAMVAAFAVSGFMHELLYYYFTRAAPTWEVTWF 321
            +ILRP  YDP R I+ R+ GPRWA+ LA+++ FAVSG MHE +YYY TR +PTWEVTWF
Sbjct: 241 TNILRPVAYDPVRRISMRILGPRWARFLAVMSTFAVSGLMHEAIYYYLTRVSPTWEVTWF 300

Query: 322 FVLHGVCTATEVAVKVAVADRKVRWRLPWAVSAPMTLAFLMVTARWLMFPQLIRNGVIET 361
           FVLHGVC A EV VK A AD   RWRL   VS P+T+ FL VT  WL FPQL+RNGV   
Sbjct: 301 FVLHGVCVAVEVEVKKAAAD---RWRLHPVVSGPLTIVFLAVTGNWLFFPQLLRNGVDLK 360

BLAST of Tan0021443 vs. TAIR 10
Match: AT5G55340.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 295.0 bits (754), Expect = 8.0e-80
Identity = 161/340 (47.35%), Postives = 212/340 (62.35%), Query Frame = 0

Query: 22  MDGELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLAIFPVLFIFLLLPLKLHYFHFCG 81
           MD EL NL+KVW  AI  I Y YY+  +I  G PRFL++ PVL +FL+LPL     H   
Sbjct: 1   MDEELKNLIKVWVSAIISISYCYYIPPRIKSGAPRFLSVSPVLALFLVLPLFFSSLHLSL 60

Query: 82  PTAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAVLCLPIKIKKH-KSPEELKNRRI 141
            TAFFL WL NFKL+LF+FD GPL   P  +   F    C PIK++++ KS   L     
Sbjct: 61  ITAFFLTWLANFKLILFSFDKGPLIPIP-TNFPRFFCFTCFPIKVQQNPKSQNHLPK--- 120

Query: 142 GVPIALSFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHMYFGIEIILALCSLPARAIIGA 201
              +  + KL + A+++ LY YR  ++P ++  LY +H+Y  IEIIL    +     +G 
Sbjct: 121 ---LVFAIKLAIFAVLLHLYSYRQNLSPTILLGLYFVHLYLEIEIILTFVKVVVFISLGC 180

Query: 202 RIEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSITSRVFGPRWAQVLAMVAA 261
            +EPQ ++PYL+TSLQDFW RRWNLMVP+ILRPAVY P R ++ R     WA    ++AA
Sbjct: 181 DLEPQSNKPYLATSLQDFWGRRWNLMVPAILRPAVYAPMRRVSERKMSSGWALFPGILAA 240

Query: 262 FAVSGFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEVAVKVAVADRKVRWRLPWAVSA 321
           F VSG +HELL++Y  R  PT EVT FFVLHGVCTA E+AVK      + RWRL   VS 
Sbjct: 241 FIVSGLVHELLFFYLIREMPTGEVTLFFVLHGVCTAVELAVKKKTTVAQ-RWRLSPGVSR 300

Query: 322 PMTLAFLMVTARWLMFPQLIRNGVIETTIGEYYVMADFVK 361
            +T+ F+ VT  WL  PQL R+GV+E    E  ++ +F+K
Sbjct: 301 VLTVGFVFVTGGWLFTPQLKRSGVMERFTSEAVLLVEFIK 332

BLAST of Tan0021443 vs. TAIR 10
Match: AT5G55350.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 294.7 bits (753), Expect = 1.0e-79
Identity = 158/342 (46.20%), Postives = 215/342 (62.87%), Query Frame = 0

Query: 22  MDGELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLAIFPVLFIFLLLPLKLHYFHFCG 81
           M+ EL +L+KVW  AI  I Y YY +++I  G+ R L++ PV  +FL+LPL +   HF G
Sbjct: 1   MEEELMSLIKVWVYAIISISYCYYTSTRIKSGVFRLLSVLPVCVLFLVLPLFVSSVHFSG 60

Query: 82  PTAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAVLCLPIKIKKHKSPEELKNRRIG 141
            TAFFL+WL NFKL+LF+FD GPL   P  +L  F    C PIK++++  P+    +   
Sbjct: 61  STAFFLSWLANFKLILFSFDQGPLFPVP-SNLSRFVCFTCFPIKLQQNPKPQNQMPK--- 120

Query: 142 VPIALSFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHMYFGIEIILALCSLPARAIIGAR 201
                + KL    +++ +Y+Y+  M+P V+ VLY LH+Y   EI+LA   +     +   
Sbjct: 121 --WGFAVKLAFFGVLLHMYEYKQHMSPTVLLVLYSLHIYLEYEILLAPLKVLLSISLWCD 180

Query: 202 IEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSITSRVFGPRWAQVLAMVAAF 261
           +EP F+EPYLSTSLQDFW RRWNLMVP+ILRPAVY P R +  R      A  L + A+F
Sbjct: 181 LEPHFNEPYLSTSLQDFWGRRWNLMVPAILRPAVYLPVRQMAGRKMNSDQALFLGVFASF 240

Query: 262 AVSGFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEVAVKVAVADRKVRWRLPWAVSAP 321
            VSG +HEL+++YFTR +PT EVT FFVLHGVCTA E A K     R  RW++   VS  
Sbjct: 241 LVSGVVHELIFFYFTRESPTGEVTLFFVLHGVCTAAECAAKRTRLVR--RWKVSQMVSRL 300

Query: 322 MTLAFLMVTARWLMFPQLIRNGVIETTIGEYYVMADFVKDKF 364
           +T+ F+++T  WL FP L R+G+IE    E ++   FVK KF
Sbjct: 301 LTVGFVVMTGGWLFFPHLARSGMIERLADEAFLFIGFVKHKF 334

BLAST of Tan0021443 vs. TAIR 10
Match: AT5G55380.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 294.3 bits (752), Expect = 1.4e-79
Identity = 158/344 (45.93%), Postives = 214/344 (62.21%), Query Frame = 0

Query: 22  MDGELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLAIFPVLFIFLLLPLKLHYFHFCG 81
           M+ +  NL++VW  A+  + Y YY++SK+ KG+ R L+I PV  +FL+LPL L   HFC 
Sbjct: 1   MEEKFRNLIEVWISALISLSYCYYISSKLSKGVLRLLSILPVCILFLVLPLFLSCVHFCA 60

Query: 82  PTAFFLAWLGNFKLLLFAFDLGPLTSTPLPSLLHFAAVLCLPIKIKKHKSPEELKNRRIG 141
            +  FL+WL NFKLLLFAFD GPL   P P L  F    CLPIKI++  SP  + N    
Sbjct: 61  ISVLFLSWLANFKLLLFAFDEGPLFPLP-PKLSRFICFACLPIKIRQDPSPNAIPNLH-P 120

Query: 142 VPI---ALSFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHMYFGIEIILALCSLPARAII 201
            P+    L+ K+L+L +++ +Y+YR+ +   V+  LYCLH+Y  +E++L         ++
Sbjct: 121 KPMPKWVLAVKILVLGVLLHVYEYRDGLPRFVVLALYCLHIYLEVELVLVFVGAVVSTLL 180

Query: 202 GARIEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSITSRVFGPRWAQVLAMV 261
           G  IEP F+EPYL+TSLQDFWSRRWNLMV ++LR  V+ P +    R+  P  A    ++
Sbjct: 181 GCNIEPVFNEPYLATSLQDFWSRRWNLMVSAVLRSTVHIPVQRFFKRILSPDGAMFAGVM 240

Query: 262 AAFAVSGFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEVAVKVAVADRKVRWRLP--W 321
           A+F VSG MHELLY+Y  R  PTWEVT FFVLHG  TATE+AVK      + +W  P   
Sbjct: 241 ASFFVSGLMHELLYFYMIRKPPTWEVTCFFVLHGAATATEIAVK------RTQWLRPPHR 300

Query: 322 AVSAPMTLAFLMVTARWLMFPQLIRNGVIETTIGEYYVMADFVK 361
           AVS  + L F+ VT  WL   Q++RN V E  IGE  ++ D  K
Sbjct: 301 AVSGLVVLTFVSVTGVWLFLAQVLRNNVHEKAIGECLLVLDLAK 336

BLAST of Tan0021443 vs. TAIR 10
Match: AT3G51970.1 (acyl-CoA sterol acyl transferase 1 )

HSP 1 Score: 293.1 bits (749), Expect = 3.0e-79
Identity = 145/334 (43.41%), Postives = 210/334 (62.87%), Query Frame = 0

Query: 28  NLVKVWSIAIALICYSYYVASKIPKGLPRFLAIFPVLFIFLLLPLKLHYFHFCGPTAFFL 87
           + +K W + I  +CY++++A  +PKG+ R +  FPV  IF ++P  ++  H  G TAFF+
Sbjct: 3   SFIKAWGLVIISLCYTFFIAKLVPKGIKRLILFFPVFLIFFIVPFLIYSLHLLGITAFFI 62

Query: 88  AWLGNFKLLLFAFDLGPLTSTPLP-SLLHFAAVLCLPIKIKKHKSPEELKNRRIGV--PI 147
           AWL NFKLLLFA   GPL+S   P SL  F AV CLPIKI+    P +  +       P+
Sbjct: 63  AWLANFKLLLFALGRGPLSSNHKPLSLPIFLAVSCLPIKIQLSPKPTKTHSHEGSTEGPL 122

Query: 148 ALSFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHMYFGIEIILALCSLPARAIIGARIEP 207
             + K + + ++I+ Y+Y  ++   V+  LY +H+YF +EIILA  +   RA+    +EP
Sbjct: 123 IYTIKAVFVVLIIKAYEYSTKLPEKVVLTLYAIHIYFALEIILAATAAAVRAMSDLELEP 182

Query: 208 QFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSITSRVFGPRWAQVLAMVAAFAVS 267
           QF++PYL+TSLQDFW RRWNLMV  ILRP VY+P+  + S V GP ++Q+LA    F VS
Sbjct: 183 QFNKPYLATSLQDFWGRRWNLMVTGILRPTVYEPSLQLFS-VLGPNYSQILAAFGTFVVS 242

Query: 268 GFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEVAVKVAVADRKVRWRLPWAVSAPMTL 327
           G MHEL+++Y  R  P W++ WFF+++G CT  E+A+K  +     RWR P A+S  +TL
Sbjct: 243 GIMHELIFFYMGRLRPDWKMMWFFLINGFCTTVEIAIKKTI---NGRWRFPKAISQVLTL 302

Query: 328 AFLMVTARWLMFPQLIRNGVIETTIGEYYVMADF 359
            F+MVTA WL  P+  R  ++E  + EY  +  F
Sbjct: 303 TFVMVTALWLFLPEFNRCNIVEKALDEYAAIGAF 332

BLAST of Tan0021443 vs. TAIR 10
Match: AT5G55360.1 (MBOAT (membrane bound O-acyl transferase) family protein )

HSP 1 Score: 286.6 bits (732), Expect = 2.8e-77
Identity = 157/345 (45.51%), Postives = 223/345 (64.64%), Query Frame = 0

Query: 22  MDGELHNLVKVWSIAIALICYSYYVASKIPKGLPRFLAIFPVLFIFLLLPLKLHYFHFCG 81
           M+ EL N +K+W  AI  I Y YY+++ I  G+ R L++ PV  +FL+ PL   Y HF G
Sbjct: 1   MEEELKNFIKLWISAIISISYCYYLSTGIKAGVFRLLSVLPVCALFLVFPLFFSYVHFSG 60

Query: 82  PTAFFLAWLGNFKLLLFAFDLGPLTSTPLP-SLLHFAAVLCLPIKIKKHKSPEELKNRRI 141
             AFFL+WL NFKL+LF+FD GPL  +PLP +L  F  + C PIK +++ + +  K    
Sbjct: 61  CMAFFLSWLANFKLILFSFDQGPL--SPLPRTLSRFICITCFPIKPQQNPNIQNYK---- 120

Query: 142 GVPIAL-SFKLLLLAIVIRLYDYRNRMNPVVIFVLYCLHMYFGIEIILALCSLPARAIIG 201
            +PI L + K+++  +++++Y+Y+  ++P ++ V   LH++  +EI+  L        +G
Sbjct: 121 -IPIWLFAIKVVIFVVLLQMYEYKQYLSPALLLVFNSLHIFLELEIVFMLVKALVFITLG 180

Query: 202 ARIEPQFDEPYLSTSLQDFWSRRWNLMVPSILRPAVYDPTRSITSRVFGPRWAQVLAMVA 261
             +EPQ +EPYL+TSLQDFW RRWNLMVP+ILRPAVY P R +  R      A  L + A
Sbjct: 181 CDLEPQSNEPYLATSLQDFWGRRWNLMVPAILRPAVYLPARRMACRKVNSDQAMFLGVFA 240

Query: 262 AFAVSGFMHELLYYYFTRAAPTWEVTWFFVLHGVCTATEVAVKVAVADRKVRWRLPWAVS 321
           AF VSG +HE+L++Y TR  PT EVTWFF+LHGVCT  EVAVK +   R+  WR+   VS
Sbjct: 241 AFLVSGAVHEMLFFYLTREVPTGEVTWFFLLHGVCTVAEVAVKKSTFVRR-WWRVSPTVS 300

Query: 322 APMTLAFLMVTARWLMFPQLIRNGVIETTIGEYYVMADFVKDKFV 365
             +T+ F++VT+ W  FP LIR+G+IE    E  +  DFVK KF+
Sbjct: 301 RLLTVGFVVVTSGWFFFP-LIRSGIIERLASEALMCIDFVKHKFL 336

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9XGY61.3e-7948.17Long-chain-alcohol O-fatty-acyltransferase OS=Simmondsia chinensis OX=3999 PE=1 ... [more]
Q9FJ761.1e-7847.35Probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Arabidopsis thaliana OX... [more]
Q9FJ751.5e-7846.20Probable long-chain-alcohol O-fatty-acyltransferase 4 OS=Arabidopsis thaliana OX... [more]
Q9FJ721.9e-7845.93Probable long-chain-alcohol O-fatty-acyltransferase 1 OS=Arabidopsis thaliana OX... [more]
Q9SV074.3e-7843.41Acyl-CoA--sterol O-acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=ASAT1 PE... [more]
Match NameE-valueIdentityDescription
KAG7037354.12.6e-16580.77putative long-chain-alcohol O-fatty-acyltransferase 4, partial [Cucurbita argyro... [more]
XP_023525242.14.9e-16481.59probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita pepo subsp. pep... [more]
XP_022981522.14.2e-16380.22probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucurbita maxima][more]
XP_038898664.14.5e-15778.36probable long-chain-alcohol O-fatty-acyltransferase 4 [Benincasa hispida][more]
XP_008448089.13.8e-14071.78PREDICTED: probable long-chain-alcohol O-fatty-acyltransferase 5 [Cucumis melo][more]
Match NameE-valueIdentityDescription
A0A6J1IU702.0e-16380.22probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucurbita maxima OX=366... [more]
A0A1S3BIX31.8e-14071.78probable long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo OX=3656 GN... [more]
A0A5A7TX122.7e-13673.10Putative long-chain-alcohol O-fatty-acyltransferase 5 OS=Cucumis melo var. makuw... [more]
A0A6J5X6S65.8e-11054.96MBOAT_2 domain-containing protein OS=Prunus armeniaca OX=36596 GN=ORAREDHAP_LOCU... [more]
A0A6J5UNH85.8e-11054.96MBOAT_2 domain-containing protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS28... [more]
Match NameE-valueIdentityDescription
AT5G55340.18.0e-8047.35MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55350.11.0e-7946.20MBOAT (membrane bound O-acyl transferase) family protein [more]
AT5G55380.11.4e-7945.93MBOAT (membrane bound O-acyl transferase) family protein [more]
AT3G51970.13.0e-7943.41acyl-CoA sterol acyl transferase 1 [more]
AT5G55360.12.8e-7745.51MBOAT (membrane bound O-acyl transferase) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032805Wax synthase domainPFAMPF13813MBOAT_2coord: 203..289
e-value: 5.2E-17
score: 61.9
IPR017088Wax synthase, MagnoliopsidaPIRSFPIRSF037006Wax_synthasecoord: 22..364
e-value: 2.1E-132
score: 439.5
IPR044851Wax synthasePANTHERPTHR31595LONG-CHAIN-ALCOHOL O-FATTY-ACYLTRANSFERASE 3-RELATEDcoord: 24..362
NoneNo IPR availablePANTHERPTHR31595:SF46ACYL-COA--STEROL O-ACYLTRANSFERASE 1coord: 24..362

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0021443.1Tan0021443.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0008374 O-acyltransferase activity