Tan0021275 (gene) Snake gourd v1

Overview
NameTan0021275
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionLysine methyltransferase
LocationLG05: 1633432 .. 1636671 (+)
RNA-Seq ExpressionTan0021275
SyntenyTan0021275
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AATTGAGGAGACCTGAAATGCACGGAACTGGAGCCTCACACTTCAAACGAAGAATTAAGAATCTCTATCAATTTGGTACGTTCCTTCGCTTTACTGAGATAAAACCAATGCTGTTTTTCAAAAAGTTAGTTTTATATAGATTAGTCTTTCACACTTTTTCTTGTGCTTGAGCAAGTGTATGATTTTGTCTCCTACCTTTCGGAAAGAAGAAATTAGTTCAAAGATTATTGTTGATGCCGTCAATCTGGTTTCTGATAGAATGCAAAAAGGTTTTAGACGATTGGGTTCGCTTTTGGGAATATTAATCTTGACTTTATTTTGTCTAGATTGTGTATGCTTGTGCAAGAGCTCCTTTAGAAATAGTTCAACATTTTCGTTCTAGTGTCACCTACTTTGTATTTATCTAATGAACTAAGTTCCTCAGGTCTCTCTCAATCCCCCCAAAACAAATGAAAATCTGAATTCTGCATTGCAGCAAATACGTTCTGATCAAGGGTTCAATTAACCTTTTGATTTTGTGGAAGTTTGAATTTTAGGTTCTTGCTATGGAGGCCGACAGGTACTATAATCTTAGCTTTCTTGTTTATTGACTATTTTGGATTATAGGCTATAATTCTCAAATAATTCATGTAATCTGTACATATCTTGAATTTAGGATTACATGTCAAAAGATGACAGTTTTCGGAATTACGGGTTCGAACTAGAATTTTTCTTACCATATGTGCTTAGTTAATCCTTTAATCTATCATTGAATTTTGTATTATCAGGTTAAATTCTCCCAGTACATTTACCATGACCATGGAAGTGTTGGGTCATGAATTGCGATTCTATCAGGTATTGCCTGGAACATTATTTTGAATCTTCATGCGCAACATTCCCTCCAGATTTGAAATTATCACTTGCCTGCCTATCATATCTACGGTACTTCAAGAATATATCTCTGTTCATTGTTGATAGCTGAGCTTGCAAAAGGCTATTGTGTAAAAAGTACTCATCCGCATTCAAATCCTCTACCCGTTGAGATTTGAGAGAGACATTTCTGTAGTCCATTGCTAATTTTTATTCCAATGTCATATCTGTAATAACCTATCTGACCATGGAATATGTGTTACATTGCGATATTATAGGATCCCAATTCGAAACATTTAGGCACAACAGTTTGGGATGCATCGCTGGTATTTGTTAAGTTTCTGGTAATCACTGGTCTTCCTTTACATTCTCTTCAACTTTCATTTGTCATTGGAGTTTTAATTTTCCTCACTCACAATATTTGGACTTACAAGTATCATCTTGCCCATGAAGACAAATTCTGGTTGTTAGTTTGTTACTCTCTGTTAATGGTATACGGCATGCCTAGCATGCTTTCGATTGGAGAAAATTTAAATCATGGCATGGCATTTATTTTGATTATTAACGGTGTTGGTTTAAAAACTGGGTGTGAGTTTTCTGACCAAGATGCAAATATTCAGAGCTAAAAGCTTTGCAGCTATTACACTTTTATGAATCCTCGATCCATCTTTGAATATAGAATGTGGAATGACATTCAATATAACTATGTTTATTCAAACACTGTCTTATCTAAATATAGTTTTACCTTGGAATTCTGGATTCAAACCCTGAGGCAGCCCTTGTAGGGTTAGTTCTAAGTTTTCATAGAACACATAATAAATACCTTTATTGAACCTGGAAGATTACTATTCTTTAATAACTTTAGAGTTCTTCGTTCATCGTTTCAGGAGAAGAATTGCCGAAAAGGAAAATTTAGCCCTTCTAAACTTAAAGGAAAGCGTGCCATTGAACTTGGAGCTGGTTGTGGGCTAGCTGGGTTTGGTGAGTTTGTTACAAAGTTTTATCCGGACCCATGTGCTTCAATGGGCTGTACCCTAATTTGAACTGGTATTTATAGGCATGGCATTGCTTGGATGTGATGTAATTGCTACAGACCAAAAGGAGGTTTTGCCTATACTAACAAGAAACATCGAACGTAATACACCAAGCTTAGCACAGATGAACCCGTCAGGTAGCTTTTTAAGATTATAAATTACAAACTTTCTTATAACATTAACATTTTTATGGTGGCAATTTAACTAAAAAAATCAGTCTGTTTGAAGTTCTGTTTAAGGTTTTAGATTCTACCACCACTATGTTTAACTTTGTCGAGGGAAATCTTCTGGTTCAATTTGATTATGCAATCTTGATAATGTTTGTAATTGTTATAGCCTTGAGAGGTTTTGATTTTCTTAATTGTTTTAACTAGGGAAACTCGTTCATTGTAGATACATTTGGTTCAATTCGTGCAGCAGAATTGGACTGGGGAAATGAAGATCATATCAAAACTGTAGGTCCACCATTTGACTTTATTATCGGCACTGATGTGGTAAGTTTATATGATTATTTCAAGTTGAAGCATACAATGGTGCTTCATTCCATAGCATGATTTTCTTATGATATACGCAGCGAATAACCGACCACGAAAGCAGTAATTTTTATTATTTGGCATGAAGGTTTATGCAGAGCATCTCTTGGAACCACTTTTGAAGACAATACATGCATTAGCAGGTCCTAAAACTACAATCATGGTATGGATGTAACTTTCATCCACATTTTCAGTGGATATTATTGTTTGCTCACTTTTTCGTACTTGTTATGTGAAGTTGGGGTATGAGATTCGTTCGACAAATGTTCATGAGCAAATGCTTGAGATGTGGAAAAAGAACTTTGAGGTGAAAACTGTATCAAAATCAAAGGTACACTATAAGCTTTCAATGCTTCTCGAGTCGGAAATGGAATTCACTCTGAAATGGCTAAATCTTAAGATGTTTGTCCTTGTTTAGTTTTAGAGAATGTCTGCACACCAATAGTCAGTTTTGTAGGATTGCCAATAGAGGAACCAGCAAGTCCTCGTTATGGTCAAATATCAAAACTGTATTACAGTTCTGATCGTGCCATTTGTTGCAGATGGACTCCCAGTACCAACATCCCAGCATTCAGCTTTACATCATGGGGCTAAAGTCTGCAGCTGGGAACACAGAGAACTCTCAAGACAATGTTGATCAAGAAATTGACAGAGGTTGTTCAAGTCAGGAGAATGATGGAAAGGATGTAAGTTGTGATGAAACGGGAACCCAGAAGGCAAAGGAGCCAGTTACAGAACACGTGGATAAGAATCTGGGCGACTGGGAAGCTAGAAGAATGGGCTCTATGGCTGCTCGTCTTCTGCAAGATATAAAAATTGCTTGA

mRNA sequence

AATTGAGGAGACCTGAAATGCACGGAACTGGAGCCTCACACTTCAAACGAAGAATTAAGAATCTCTATCAATTTGGTCTCTCTCAATCCCCCCAAAACAAATGAAAATCTGAATTCTGCATTGCAGCAAATACGTTCTGATCAAGGGTTCAATTAACCTTTTGATTTTGTGGAAGTTTGAATTTTAGGTTCTTGCTATGGAGGCCGACAGGTTAAATTCTCCCAGTACATTTACCATGACCATGGAAGTGTTGGGTCATGAATTGCGATTCTATCAGGTATTGCCTGGAACATTATTTTGAATCTTCATGCGCAACATTCCCTCCAGATTTGAAATTATCACTTGCCTGCCTATCATATCTACGGATCCCAATTCGAAACATTTAGGCACAACAGTTTGGGATGCATCGCTGGTATTTGTTAAGTTTCTGGAGAAGAATTGCCGAAAAGGAAAATTTAGCCCTTCTAAACTTAAAGGAAAGCGTGCCATTGAACTTGGAGCTGGTTGTGGGCTAGCTGGGTTTGGCATGGCATTGCTTGGATGTGATGTAATTGCTACAGACCAAAAGGAGGTTTTGCCTATACTAACAAGAAACATCGAACGTAATACACCAAGCTTAGCACAGATGAACCCGTCAGATACATTTGGTTCAATTCGTGCAGCAGAATTGGACTGGGGAAATGAAGATCATATCAAAACTGTAGGTCCACCATTTGACTTTATTATCGGCACTGATGTGGTTTATGCAGAGCATCTCTTGGAACCACTTTTGAAGACAATACATGCATTAGCAGGTCCTAAAACTACAATCATGTTGGGGTATGAGATTCGTTCGACAAATGTTCATGAGCAAATGCTTGAGATGTGGAAAAAGAACTTTGAGGTGAAAACTGTATCAAAATCAAAGATGGACTCCCAGTACCAACATCCCAGCATTCAGCTTTACATCATGGGGCTAAAGTCTGCAGCTGGGAACACAGAGAACTCTCAAGACAATGTTGATCAAGAAATTGACAGAGGTTGTTCAAGTCAGGAGAATGATGGAAAGGATGTAAGTTGTGATGAAACGGGAACCCAGAAGGCAAAGGAGCCAGTTACAGAACACGTGGATAAGAATCTGGGCGACTGGGAAGCTAGAAGAATGGGCTCTATGGCTGCTCGTCTTCTGCAAGATATAAAAATTGCTTGA

Coding sequence (CDS)

ATGCGCAACATTCCCTCCAGATTTGAAATTATCACTTGCCTGCCTATCATATCTACGGATCCCAATTCGAAACATTTAGGCACAACAGTTTGGGATGCATCGCTGGTATTTGTTAAGTTTCTGGAGAAGAATTGCCGAAAAGGAAAATTTAGCCCTTCTAAACTTAAAGGAAAGCGTGCCATTGAACTTGGAGCTGGTTGTGGGCTAGCTGGGTTTGGCATGGCATTGCTTGGATGTGATGTAATTGCTACAGACCAAAAGGAGGTTTTGCCTATACTAACAAGAAACATCGAACGTAATACACCAAGCTTAGCACAGATGAACCCGTCAGATACATTTGGTTCAATTCGTGCAGCAGAATTGGACTGGGGAAATGAAGATCATATCAAAACTGTAGGTCCACCATTTGACTTTATTATCGGCACTGATGTGGTTTATGCAGAGCATCTCTTGGAACCACTTTTGAAGACAATACATGCATTAGCAGGTCCTAAAACTACAATCATGTTGGGGTATGAGATTCGTTCGACAAATGTTCATGAGCAAATGCTTGAGATGTGGAAAAAGAACTTTGAGGTGAAAACTGTATCAAAATCAAAGATGGACTCCCAGTACCAACATCCCAGCATTCAGCTTTACATCATGGGGCTAAAGTCTGCAGCTGGGAACACAGAGAACTCTCAAGACAATGTTGATCAAGAAATTGACAGAGGTTGTTCAAGTCAGGAGAATGATGGAAAGGATGTAAGTTGTGATGAAACGGGAACCCAGAAGGCAAAGGAGCCAGTTACAGAACACGTGGATAAGAATCTGGGCGACTGGGAAGCTAGAAGAATGGGCTCTATGGCTGCTCGTCTTCTGCAAGATATAAAAATTGCTTGA

Protein sequence

MRNIPSRFEIITCLPIISTDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGCDVIATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGPPFDFIIGTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKSKMDSQYQHPSIQLYIMGLKSAAGNTENSQDNVDQEIDRGCSSQENDGKDVSCDETGTQKAKEPVTEHVDKNLGDWEARRMGSMAARLLQDIKIA
Homology
BLAST of Tan0021275 vs. ExPASy Swiss-Prot
Match: Q5BLD8 (Protein N-lysine methyltransferase METTL21A OS=Danio rerio OX=7955 GN=mettl21a PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.6e-19
Identity = 65/180 (36.11%), Postives = 90/180 (50.00%), Query Frame = 0

Query: 17  ISTDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMAL 76
           +S D     +   VWDA++V   FLE     GK     LKGKR IELGAG GL G   AL
Sbjct: 33  LSQDWKRLGVAAVVWDAAVVLCMFLE----MGKVD---LKGKRVIELGAGTGLVGIVAAL 92

Query: 77  LGCDVIATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGPPF 136
           LG +V  TD++  L  LT N+  N P   Q        +++ +EL WG    +   G  +
Sbjct: 93  LGANVTITDREPALEFLTANVHENIPQGRQK-------AVQVSELTWGENLDLYPQG-GY 152

Query: 137 DFIIGTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTV 196
           D I+G D+VY E     LL+T+  L+   T ++L   IR     E+ L   ++ F V+ V
Sbjct: 153 DLILGADIVYLEETFPALLQTLEHLSSGDTVVLLSCRIRYER-DERFLTELRQRFSVQEV 196

BLAST of Tan0021275 vs. ExPASy Swiss-Prot
Match: Q8WXB1 (Protein N-lysine methyltransferase METTL21A OS=Homo sapiens OX=9606 GN=METTL21A PE=1 SV=2)

HSP 1 Score: 88.2 bits (217), Expect = 1.7e-16
Identity = 60/176 (34.09%), Postives = 84/176 (47.73%), Query Frame = 0

Query: 24  KHLG--TTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGCDV 83
           +HLG    VWDA++V   +LE           +L+G+ A+ELGAG GL G   ALLG  V
Sbjct: 38  RHLGVAAVVWDAAIVLSTYLE-------MGAVELRGRSAVELGAGTGLVGIVAALLGAHV 97

Query: 84  IATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGP-PFDFII 143
             TD+K  L  L  N++ N P   Q             EL WG   ++ +  P  FD I+
Sbjct: 98  TITDRKVALEFLKSNVQANLPPHIQTKTV-------VKELTWG--QNLGSFSPGEFDLIL 157

Query: 144 GTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTV 197
           G D++Y E     LL+T+  L    + I+L   IR        L M ++ F V+ V
Sbjct: 158 GADIIYLEETFTDLLQTLEHLCSNHSVILLACRIRYER-DNNFLAMLERQFTVRKV 196

BLAST of Tan0021275 vs. ExPASy Swiss-Prot
Match: A4FV42 (Protein N-lysine methyltransferase METTL21A OS=Bos taurus OX=9913 GN=METTL21A PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.9e-15
Identity = 63/183 (34.43%), Postives = 85/183 (46.45%), Query Frame = 0

Query: 24  KHLG--TTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGCDV 83
           K LG    VWDA++V   +LE           +L+G  A+ELGAG GL G   ALLG  V
Sbjct: 38  KQLGVAAVVWDAAVVLATYLE-------MGTVELRGCSAVELGAGTGLVGIVAALLGAHV 97

Query: 84  IATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGPPFDFIIG 143
             TD+K  L  L  N++ N P   Q             EL WG      + G  FD I+G
Sbjct: 98  TITDRKVALEFLKSNVQANLPPHIQPKAV-------VKELTWGQNLGRFSPG-EFDLILG 157

Query: 144 TDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKSKM 203
            D++Y E     LL+T+  L    + ++L   IR    +   L M ++ F   TVSK   
Sbjct: 158 ADIIYLEETFTDLLQTLEHLCSNHSVVLLACRIRYERDY-NFLAMLERQF---TVSKVHY 201

Query: 204 DSQ 205
           DS+
Sbjct: 218 DSE 201

BLAST of Tan0021275 vs. ExPASy Swiss-Prot
Match: Q9CQL0 (Protein N-lysine methyltransferase METTL21A OS=Mus musculus OX=10090 GN=Mettl21A PE=2 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.9e-15
Identity = 62/180 (34.44%), Postives = 84/180 (46.67%), Query Frame = 0

Query: 26  LGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGCDVIATD 85
           +   VWDA++V   +LE           +L+G  A+ELGAG GL G   ALLG  V  TD
Sbjct: 42  VAAVVWDAAVVLSMYLE-------MGAVELRGCSAVELGAGTGLVGIVAALLGAQVTITD 101

Query: 86  QKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGP-PFDFIIGTDV 145
           +K  L  L  N+E N P   Q             EL WG   ++++  P  FD I+G DV
Sbjct: 102 RKVALEFLKSNVEANLPPHIQPKAV-------VKELTWG--QNLESFSPGEFDLILGADV 161

Query: 146 VYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKSKMDSQ 205
           +Y E     LL+T+  L    + I+L   IR        L M ++ F   TVSK   D +
Sbjct: 162 IYLEDTFTDLLQTLGHLCSNNSVILLACRIRYER-DSNFLTMLERQF---TVSKVHYDPE 201

BLAST of Tan0021275 vs. ExPASy Swiss-Prot
Match: A4IGU3 (Protein N-lysine methyltransferase METTL21A OS=Xenopus tropicalis OX=8364 GN=mettl21a PE=2 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.2e-15
Identity = 58/185 (31.35%), Postives = 82/185 (44.32%), Query Frame = 0

Query: 17  ISTDPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMAL 76
           I  D     +   VWDA+LV   +LE            L+    IELGAG GL G   AL
Sbjct: 33  IKQDWKQLGVAAVVWDAALVLCMYLES-------EGIHLQNSSVIELGAGTGLVGIVAAL 92

Query: 77  LGCDVIATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNE-DHIKTVGPP 136
           LG  V  TD+   +  L  N+  N        P D+   +    L+WG   +   T    
Sbjct: 93  LGAQVTITDRDLAMEFLRMNVRDNI-------PKDSLHRVSVRALNWGKSLEEFST---- 152

Query: 137 FDFIIGTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKT 196
           +DFI+G D++Y E     LL+T   L+  ++ I+L   +R    H+  LEM K +F +  
Sbjct: 153 YDFILGADIIYLEETFPDLLQTFLHLSSQQSVILLSSRLRYQRDHD-FLEMMKLHFTIAD 198

Query: 197 VSKSK 201
           V   K
Sbjct: 213 VYYDK 198

BLAST of Tan0021275 vs. NCBI nr
Match: KAG6570813.1 (Protein-lysine methyltransferase METTL21D, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 508.8 bits (1309), Expect = 3.0e-140
Identity = 249/273 (91.21%), Postives = 261/273 (95.60%), Query Frame = 0

Query: 20  DPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGC 79
           DPNSKHLGTTVWDASLVFVKFLE+NCRKGKFSPSKLKGKR +ELGAGCGLAGFGMALLGC
Sbjct: 97  DPNSKHLGTTVWDASLVFVKFLERNCRKGKFSPSKLKGKRVVELGAGCGLAGFGMALLGC 156

Query: 80  DVIATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGPPFDFI 139
           DVIATDQKEVLPIL+RNIERNTPSLAQMNPSD+FGSIRAAELDWGNEDHIK VGPPFDFI
Sbjct: 157 DVIATDQKEVLPILSRNIERNTPSLAQMNPSDSFGSIRAAELDWGNEDHIKAVGPPFDFI 216

Query: 140 IGTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 199
           IGTDVVYAEHLLEPLLKTIHAL+GPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS
Sbjct: 217 IGTDVVYAEHLLEPLLKTIHALSGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 276

Query: 200 KMDSQYQHPSIQLYIMGLKSAAGNTENSQDNVDQEIDRGCSSQENDGKDVSCDETGTQKA 259
           KMDSQYQHPSIQLYIMG+KSAA N EN+QDNVDQEIDR CSSQEND KDVSCD TG QK 
Sbjct: 277 KMDSQYQHPSIQLYIMGMKSAAENAENTQDNVDQEIDRVCSSQENDEKDVSCDGTGIQKV 336

Query: 260 KEPVTEHVDKNLGDWEARRMGSMAARLLQDIKI 293
           +EPV+E  D+ LGDWEARR+GSMAARLLQD+KI
Sbjct: 337 EEPVSEREDQKLGDWEARRIGSMAARLLQDVKI 369

BLAST of Tan0021275 vs. NCBI nr
Match: XP_022944009.1 (protein-lysine methyltransferase METTL21D [Cucurbita moschata] >XP_022944011.1 protein-lysine methyltransferase METTL21D [Cucurbita moschata])

HSP 1 Score: 508.4 bits (1308), Expect = 4.0e-140
Identity = 248/273 (90.84%), Postives = 261/273 (95.60%), Query Frame = 0

Query: 20  DPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGC 79
           DPNSKHLGTTVWDASLVFVKFLE+NCRKGKFSPSKLKGKR +ELGAGCGLAGFGMALLGC
Sbjct: 28  DPNSKHLGTTVWDASLVFVKFLERNCRKGKFSPSKLKGKRVVELGAGCGLAGFGMALLGC 87

Query: 80  DVIATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGPPFDFI 139
           DV+ATDQKEVLPIL+RNIERNTPSLAQMNPSD+FGSIRAAELDWGNEDHIK VGPPFDFI
Sbjct: 88  DVVATDQKEVLPILSRNIERNTPSLAQMNPSDSFGSIRAAELDWGNEDHIKAVGPPFDFI 147

Query: 140 IGTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 199
           IGTDVVYAEHLLEPLLKTIHAL+GPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS
Sbjct: 148 IGTDVVYAEHLLEPLLKTIHALSGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 207

Query: 200 KMDSQYQHPSIQLYIMGLKSAAGNTENSQDNVDQEIDRGCSSQENDGKDVSCDETGTQKA 259
           KMDSQYQHPSIQLYIMG+KSAA N EN+QDNVDQEIDR CSSQEND KDVSCD TG QK 
Sbjct: 208 KMDSQYQHPSIQLYIMGMKSAAENAENTQDNVDQEIDRVCSSQENDEKDVSCDGTGIQKV 267

Query: 260 KEPVTEHVDKNLGDWEARRMGSMAARLLQDIKI 293
           +EPV+E  D+ LGDWEARR+GSMAARLLQD+KI
Sbjct: 268 EEPVSEREDQKLGDWEARRIGSMAARLLQDVKI 300

BLAST of Tan0021275 vs. NCBI nr
Match: XP_023512824.1 (protein N-lysine methyltransferase METTL21A [Cucurbita pepo subsp. pepo] >XP_023512825.1 protein N-lysine methyltransferase METTL21A [Cucurbita pepo subsp. pepo] >XP_023512826.1 protein N-lysine methyltransferase METTL21A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 507.3 bits (1305), Expect = 8.9e-140
Identity = 251/276 (90.94%), Postives = 262/276 (94.93%), Query Frame = 0

Query: 20  DPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGC 79
           DPNSKHLGTTVWDASLVFVKFLE+NCRKGKFSPSKLKGKR +ELGAGCGLAGFGMALLGC
Sbjct: 28  DPNSKHLGTTVWDASLVFVKFLERNCRKGKFSPSKLKGKRVVELGAGCGLAGFGMALLGC 87

Query: 80  DVIATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGPPFDFI 139
           DVIATDQKEVLPIL+RNIERNTPSLAQMNPSD+FGSIRAAELDWGNEDHIK VGPPFDFI
Sbjct: 88  DVIATDQKEVLPILSRNIERNTPSLAQMNPSDSFGSIRAAELDWGNEDHIKAVGPPFDFI 147

Query: 140 IGTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 199
           IGTDVVYAEHLLEPLLKTIHAL+GPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS
Sbjct: 148 IGTDVVYAEHLLEPLLKTIHALSGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 207

Query: 200 KMDSQYQHPSIQLYIMGLKSA---AGNTENSQDNVDQEIDRGCSSQENDGKDVSCDETGT 259
           KMDSQYQHPSIQLYIMG+KSA   A N EN+QDNVDQEIDR CSSQEND KDVSCD TG 
Sbjct: 208 KMDSQYQHPSIQLYIMGMKSAAENAENAENTQDNVDQEIDRVCSSQENDEKDVSCDGTGI 267

Query: 260 QKAKEPVTEHVDKNLGDWEARRMGSMAARLLQDIKI 293
           QKA EPV+EH D+ LGDWEARR+GSMAARLLQD+KI
Sbjct: 268 QKADEPVSEHEDQKLGDWEARRIGSMAARLLQDVKI 303

BLAST of Tan0021275 vs. NCBI nr
Match: KAG7010654.1 (Protein-lysine methyltransferase METTL21D [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 506.1 bits (1302), Expect = 2.0e-139
Identity = 248/273 (90.84%), Postives = 260/273 (95.24%), Query Frame = 0

Query: 20  DPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGC 79
           DPNSKHLGTTVWDASLVFVKFLE+NCRKGKFSPSKLKGKR +ELGAGCGLAGFGMALLGC
Sbjct: 28  DPNSKHLGTTVWDASLVFVKFLERNCRKGKFSPSKLKGKRVVELGAGCGLAGFGMALLGC 87

Query: 80  DVIATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGPPFDFI 139
           DVIATDQKEVLPIL+RNIERNTPSLAQMNPSD+FGSIRAAELDWGNEDHIK VGPPFDFI
Sbjct: 88  DVIATDQKEVLPILSRNIERNTPSLAQMNPSDSFGSIRAAELDWGNEDHIKAVGPPFDFI 147

Query: 140 IGTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 199
           IGTDVVYAEHLLEPLLKTIHAL+GPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS
Sbjct: 148 IGTDVVYAEHLLEPLLKTIHALSGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 207

Query: 200 KMDSQYQHPSIQLYIMGLKSAAGNTENSQDNVDQEIDRGCSSQENDGKDVSCDETGTQKA 259
           KMDSQYQHPSIQLYIMG+KSAA N EN+QDNVDQEIDR CSSQEN  KDVSCD TG QK 
Sbjct: 208 KMDSQYQHPSIQLYIMGMKSAAENAENTQDNVDQEIDRVCSSQENGEKDVSCDGTGIQKV 267

Query: 260 KEPVTEHVDKNLGDWEARRMGSMAARLLQDIKI 293
           +EPV+E  D+ LGDWEARR+GSMAARLLQD+KI
Sbjct: 268 EEPVSEREDQKLGDWEARRIGSMAARLLQDVKI 300

BLAST of Tan0021275 vs. NCBI nr
Match: XP_022140387.1 (protein N-lysine methyltransferase METTL21A [Momordica charantia])

HSP 1 Score: 500.7 bits (1288), Expect = 8.3e-138
Identity = 246/275 (89.45%), Postives = 260/275 (94.55%), Query Frame = 0

Query: 20  DPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGC 79
           DPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKR IELGAGCGLAGFGMALLGC
Sbjct: 28  DPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRVIELGAGCGLAGFGMALLGC 87

Query: 80  DVIATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGPPFDFI 139
           DV+ATDQKEVLPILTRNIERNTPS AQMNPSD+FGSI AAELDWGNEDHIK VGPPFDFI
Sbjct: 88  DVVATDQKEVLPILTRNIERNTPSFAQMNPSDSFGSISAAELDWGNEDHIKAVGPPFDFI 147

Query: 140 IGTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 199
           IGTDVVYAEHLLEPLL TIHAL+GPKTTIMLGYEIRSTNVHEQML+MWKKNFEVKTVSKS
Sbjct: 148 IGTDVVYAEHLLEPLLMTIHALSGPKTTIMLGYEIRSTNVHEQMLDMWKKNFEVKTVSKS 207

Query: 200 KMDSQYQHPSIQLYIMGLKSAAGNTENSQ-DNVDQEIDRGCSSQENDGKDVSCDETGTQK 259
           KMDSQYQHP+IQLYIMGLKSAAGNTEN Q DN+DQ+IDR CSS+END KDVSCDET  QK
Sbjct: 208 KMDSQYQHPNIQLYIMGLKSAAGNTENPQEDNIDQKIDRVCSSRENDKKDVSCDETDIQK 267

Query: 260 AKEPVTEHVDKNLGDWEARRMGSMAARLLQDIKIA 294
           A+EPVTE+ D+ + DWE RR+GSMAARLLQD+KIA
Sbjct: 268 AEEPVTENADEKVSDWEVRRIGSMAARLLQDVKIA 302

BLAST of Tan0021275 vs. ExPASy TrEMBL
Match: A0A6J1FT98 (protein-lysine methyltransferase METTL21D OS=Cucurbita moschata OX=3662 GN=LOC111448568 PE=4 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 1.9e-140
Identity = 248/273 (90.84%), Postives = 261/273 (95.60%), Query Frame = 0

Query: 20  DPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGC 79
           DPNSKHLGTTVWDASLVFVKFLE+NCRKGKFSPSKLKGKR +ELGAGCGLAGFGMALLGC
Sbjct: 28  DPNSKHLGTTVWDASLVFVKFLERNCRKGKFSPSKLKGKRVVELGAGCGLAGFGMALLGC 87

Query: 80  DVIATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGPPFDFI 139
           DV+ATDQKEVLPIL+RNIERNTPSLAQMNPSD+FGSIRAAELDWGNEDHIK VGPPFDFI
Sbjct: 88  DVVATDQKEVLPILSRNIERNTPSLAQMNPSDSFGSIRAAELDWGNEDHIKAVGPPFDFI 147

Query: 140 IGTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 199
           IGTDVVYAEHLLEPLLKTIHAL+GPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS
Sbjct: 148 IGTDVVYAEHLLEPLLKTIHALSGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 207

Query: 200 KMDSQYQHPSIQLYIMGLKSAAGNTENSQDNVDQEIDRGCSSQENDGKDVSCDETGTQKA 259
           KMDSQYQHPSIQLYIMG+KSAA N EN+QDNVDQEIDR CSSQEND KDVSCD TG QK 
Sbjct: 208 KMDSQYQHPSIQLYIMGMKSAAENAENTQDNVDQEIDRVCSSQENDEKDVSCDGTGIQKV 267

Query: 260 KEPVTEHVDKNLGDWEARRMGSMAARLLQDIKI 293
           +EPV+E  D+ LGDWEARR+GSMAARLLQD+KI
Sbjct: 268 EEPVSEREDQKLGDWEARRIGSMAARLLQDVKI 300

BLAST of Tan0021275 vs. ExPASy TrEMBL
Match: A0A6J1CGP5 (protein N-lysine methyltransferase METTL21A OS=Momordica charantia OX=3673 GN=LOC111011074 PE=4 SV=1)

HSP 1 Score: 500.7 bits (1288), Expect = 4.0e-138
Identity = 246/275 (89.45%), Postives = 260/275 (94.55%), Query Frame = 0

Query: 20  DPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGC 79
           DPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKR IELGAGCGLAGFGMALLGC
Sbjct: 28  DPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRVIELGAGCGLAGFGMALLGC 87

Query: 80  DVIATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGPPFDFI 139
           DV+ATDQKEVLPILTRNIERNTPS AQMNPSD+FGSI AAELDWGNEDHIK VGPPFDFI
Sbjct: 88  DVVATDQKEVLPILTRNIERNTPSFAQMNPSDSFGSISAAELDWGNEDHIKAVGPPFDFI 147

Query: 140 IGTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 199
           IGTDVVYAEHLLEPLL TIHAL+GPKTTIMLGYEIRSTNVHEQML+MWKKNFEVKTVSKS
Sbjct: 148 IGTDVVYAEHLLEPLLMTIHALSGPKTTIMLGYEIRSTNVHEQMLDMWKKNFEVKTVSKS 207

Query: 200 KMDSQYQHPSIQLYIMGLKSAAGNTENSQ-DNVDQEIDRGCSSQENDGKDVSCDETGTQK 259
           KMDSQYQHP+IQLYIMGLKSAAGNTEN Q DN+DQ+IDR CSS+END KDVSCDET  QK
Sbjct: 208 KMDSQYQHPNIQLYIMGLKSAAGNTENPQEDNIDQKIDRVCSSRENDKKDVSCDETDIQK 267

Query: 260 AKEPVTEHVDKNLGDWEARRMGSMAARLLQDIKIA 294
           A+EPVTE+ D+ + DWE RR+GSMAARLLQD+KIA
Sbjct: 268 AEEPVTENADEKVSDWEVRRIGSMAARLLQDVKIA 302

BLAST of Tan0021275 vs. ExPASy TrEMBL
Match: A0A0A0KGI7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G147640 PE=4 SV=1)

HSP 1 Score: 496.1 bits (1276), Expect = 9.9e-137
Identity = 246/281 (87.54%), Postives = 257/281 (91.46%), Query Frame = 0

Query: 20  DPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGC 79
           DPNSKHLGTTVWDASLVFVKFLE+NCRKGKFSPSKLKGKR IELGAGCGLAGFGMALLGC
Sbjct: 28  DPNSKHLGTTVWDASLVFVKFLERNCRKGKFSPSKLKGKRVIELGAGCGLAGFGMALLGC 87

Query: 80  DVIATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGPPFDFI 139
           DV+ATDQ+EVLPIL+RNIERNTPSLAQMNPSD+FGSIRAAELDWGNEDHIK VGPPFDFI
Sbjct: 88  DVVATDQREVLPILSRNIERNTPSLAQMNPSDSFGSIRAAELDWGNEDHIKAVGPPFDFI 147

Query: 140 IGTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 199
           IGTDVVYAEHLLEPLL TIHAL+GPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVS+S
Sbjct: 148 IGTDVVYAEHLLEPLLMTIHALSGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSQS 207

Query: 200 KMDSQYQHPSIQLYIMGLKSAAGNTENSQDNVDQEIDRGCSSQENDGKDV--------SC 259
           KMDSQYQHPSIQLYIMGLKS AGN +N+  NV+QEID  CS QEND KDV        SC
Sbjct: 208 KMDSQYQHPSIQLYIMGLKSRAGNAKNTPGNVEQEIDTVCSRQENDEKDVSFNDEKDISC 267

Query: 260 DETGTQKAKEPVTEHVDKNLGDWEARRMGSMAARLLQDIKI 293
           DETG QK  EPVTEH DK LGDWEARRMGSMAARLLQD+KI
Sbjct: 268 DETGVQKTDEPVTEHKDKKLGDWEARRMGSMAARLLQDVKI 308

BLAST of Tan0021275 vs. ExPASy TrEMBL
Match: A0A1S4E579 (protein N-lysine methyltransferase METTL21A OS=Cucumis melo OX=3656 GN=LOC103502991 PE=4 SV=1)

HSP 1 Score: 493.0 bits (1268), Expect = 8.4e-136
Identity = 244/281 (86.83%), Postives = 258/281 (91.81%), Query Frame = 0

Query: 20  DPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGC 79
           DPNSKHLGTTVWDASLVFVKFLE+NCRKGKFSPSKLKGKR IELGAGCGLAGFGMALLGC
Sbjct: 28  DPNSKHLGTTVWDASLVFVKFLERNCRKGKFSPSKLKGKRVIELGAGCGLAGFGMALLGC 87

Query: 80  DVIATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGPPFDFI 139
           DV+ATDQ+EVLPIL+RNIERNTPSLAQMNPSD+FGSI+AAELDWGNEDHIK VGPPFDFI
Sbjct: 88  DVVATDQREVLPILSRNIERNTPSLAQMNPSDSFGSIKAAELDWGNEDHIKAVGPPFDFI 147

Query: 140 IGTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 199
           IGTDVVYAEHLLEPLLKTIHAL+GPKTTIMLGYEIRSTNVHEQMLEMWK NFEVKTVS+S
Sbjct: 148 IGTDVVYAEHLLEPLLKTIHALSGPKTTIMLGYEIRSTNVHEQMLEMWKTNFEVKTVSQS 207

Query: 200 KMDSQYQHPSIQLYIMGLKSAAGNTENSQDNVDQEIDRGCSSQEND--------GKDVSC 259
           KMDSQYQHPSIQLYIMG+KS AGN +N+Q NV+QEID  CS QEND         KDVS 
Sbjct: 208 KMDSQYQHPSIQLYIMGIKSGAGNAKNTQGNVEQEIDTVCSRQENDETDVSFNNEKDVSY 267

Query: 260 DETGTQKAKEPVTEHVDKNLGDWEARRMGSMAARLLQDIKI 293
           DETG QK +EPVTEH DK LGDWEARRMGSMAARLLQD+KI
Sbjct: 268 DETGIQKTEEPVTEHKDKKLGDWEARRMGSMAARLLQDVKI 308

BLAST of Tan0021275 vs. ExPASy TrEMBL
Match: A0A6J1JBP5 (protein N-lysine methyltransferase METTL21A OS=Cucurbita maxima OX=3661 GN=LOC111483605 PE=4 SV=1)

HSP 1 Score: 484.6 bits (1246), Expect = 3.0e-133
Identity = 240/273 (87.91%), Postives = 254/273 (93.04%), Query Frame = 0

Query: 20  DPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGC 79
           DPNSKHLGTTVWDASLVFVKFLE+NCRKGKFSPSKLKGKR +ELGAGCGLAGFGMALLGC
Sbjct: 28  DPNSKHLGTTVWDASLVFVKFLERNCRKGKFSPSKLKGKRVVELGAGCGLAGFGMALLGC 87

Query: 80  DVIATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGPPFDFI 139
           DV+ATDQKEVLPIL+RNIERNTPSLAQMNPSD+FGSIRAAELDWGNEDHIK VGPPFDFI
Sbjct: 88  DVVATDQKEVLPILSRNIERNTPSLAQMNPSDSFGSIRAAELDWGNEDHIKAVGPPFDFI 147

Query: 140 IGTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 199
           IGTDVVYAEHLLEPLLKTIHAL+GPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS
Sbjct: 148 IGTDVVYAEHLLEPLLKTIHALSGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 207

Query: 200 KMDSQYQHPSIQLYIMGLKSAAGNTENSQDNVDQEIDRGCSSQENDGKDVSCDETGTQKA 259
           KMDSQYQHPSIQLYIM LKSAA N E +++NVDQEIDR   SQE D KDVSCD  G QKA
Sbjct: 208 KMDSQYQHPSIQLYIMVLKSAAENAEKTRENVDQEIDRVFLSQEIDEKDVSCDGKGIQKA 267

Query: 260 KEPVTEHVDKNLGDWEARRMGSMAARLLQDIKI 293
            EPV+   D+ LGDWEARR+GS+AARLLQD+KI
Sbjct: 268 DEPVSGREDQKLGDWEARRIGSVAARLLQDVKI 300

BLAST of Tan0021275 vs. TAIR 10
Match: AT1G08125.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 343.2 bits (879), Expect = 2.1e-94
Identity = 177/288 (61.46%), Postives = 213/288 (73.96%), Query Frame = 0

Query: 20  DPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGC 79
           DPNSKHLGTTVWDAS+VF K+L KN RKG+FS SKLKGKRAIELGAGCG+AGF +A+LGC
Sbjct: 28  DPNSKHLGTTVWDASMVFAKYLGKNSRKGRFSSSKLKGKRAIELGAGCGVAGFALAMLGC 87

Query: 80  DVIATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGPPFDFI 139
           DV+ TDQKEVLP+L RN+E NT  + QMNP   FGS+R AELDWGNEDHI  V PPFD++
Sbjct: 88  DVVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSAFGSLRVAELDWGNEDHITAVEPPFDYV 147

Query: 140 IGTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 199
           IGTDVVY+E LLEPLL+TI AL+GPKTT+MLGYEIRST VHE+ML+MWK NFEVKT+ +S
Sbjct: 148 IGTDVVYSEQLLEPLLRTILALSGPKTTVMLGYEIRSTVVHEKMLQMWKDNFEVKTIPRS 207

Query: 200 KMDSQYQHPSIQLYIMGLKSAAGNTEN-SQDNVDQEIDR-GCSSQENDGKDVSCDETGTQ 259
           KMD +YQ PSI LYIM  KSAA ++ N S+D V  + D   C ++      V   E   +
Sbjct: 208 KMDGEYQDPSIHLYIMAQKSAAESSGNVSRDEVVIDTDETKCETK------VPTGECHKR 267

Query: 260 KAKEPVTEH-------------VDKNLGDWEARRMGSMAARLLQDIKI 293
             +E V  H              D  L +WE RR G++AA+LL+D+KI
Sbjct: 268 VEEEAVVTHGPRLNEDSLLMRLRDGKLSEWEMRRYGTVAAQLLRDVKI 309

BLAST of Tan0021275 vs. TAIR 10
Match: AT1G08125.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 343.2 bits (879), Expect = 2.1e-94
Identity = 177/288 (61.46%), Postives = 213/288 (73.96%), Query Frame = 0

Query: 20  DPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGC 79
           DPNSKHLGTTVWDAS+VF K+L KN RKG+FS SKLKGKRAIELGAGCG+AGF +A+LGC
Sbjct: 35  DPNSKHLGTTVWDASMVFAKYLGKNSRKGRFSSSKLKGKRAIELGAGCGVAGFALAMLGC 94

Query: 80  DVIATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGPPFDFI 139
           DV+ TDQKEVLP+L RN+E NT  + QMNP   FGS+R AELDWGNEDHI  V PPFD++
Sbjct: 95  DVVTTDQKEVLPLLKRNVEWNTSRIVQMNPGSAFGSLRVAELDWGNEDHITAVEPPFDYV 154

Query: 140 IGTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 199
           IGTDVVY+E LLEPLL+TI AL+GPKTT+MLGYEIRST VHE+ML+MWK NFEVKT+ +S
Sbjct: 155 IGTDVVYSEQLLEPLLRTILALSGPKTTVMLGYEIRSTVVHEKMLQMWKDNFEVKTIPRS 214

Query: 200 KMDSQYQHPSIQLYIMGLKSAAGNTEN-SQDNVDQEIDR-GCSSQENDGKDVSCDETGTQ 259
           KMD +YQ PSI LYIM  KSAA ++ N S+D V  + D   C ++      V   E   +
Sbjct: 215 KMDGEYQDPSIHLYIMAQKSAAESSGNVSRDEVVIDTDETKCETK------VPTGECHKR 274

Query: 260 KAKEPVTEH-------------VDKNLGDWEARRMGSMAARLLQDIKI 293
             +E V  H              D  L +WE RR G++AA+LL+D+KI
Sbjct: 275 VEEEAVVTHGPRLNEDSLLMRLRDGKLSEWEMRRYGTVAAQLLRDVKI 316

BLAST of Tan0021275 vs. TAIR 10
Match: AT1G08125.3 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 218.0 bits (554), Expect = 1.0e-56
Identity = 134/288 (46.53%), Postives = 163/288 (56.60%), Query Frame = 0

Query: 20  DPNSKHLGTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGC 79
           DPNSKHLGTTVWDAS+VF K+L KN RKG+FS SKLKGKRAIELGAGCG+AGF +A+LGC
Sbjct: 52  DPNSKHLGTTVWDASMVFAKYLGKNSRKGRFSSSKLKGKRAIELGAGCGVAGFALAMLGC 111

Query: 80  DVIATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGPPFDFI 139
                                                                       
Sbjct: 112 ------------------------------------------------------------ 171

Query: 140 IGTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKS 199
              DVVY+E LLEPLL+TI AL+GPKTT+MLGYEIRST VHE+ML+MWK NFEVKT+ +S
Sbjct: 172 ---DVVYSEQLLEPLLRTILALSGPKTTVMLGYEIRSTVVHEKMLQMWKDNFEVKTIPRS 231

Query: 200 KMDSQYQHPSIQLYIMGLKSAAGNTEN-SQDNVDQEIDR-GCSSQENDGKDVSCDETGTQ 259
           KMD +YQ PSI LYIM  KSAA ++ N S+D V  + D   C ++      V   E   +
Sbjct: 232 KMDGEYQDPSIHLYIMAQKSAAESSGNVSRDEVVIDTDETKCETK------VPTGECHKR 270

Query: 260 KAKEPVTEH-------------VDKNLGDWEARRMGSMAARLLQDIKI 293
             +E V  H              D  L +WE RR G++AA+LL+D+KI
Sbjct: 292 VEEEAVVTHGPRLNEDSLLMRLRDGKLSEWEMRRYGTVAAQLLRDVKI 270

BLAST of Tan0021275 vs. TAIR 10
Match: AT5G44170.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 117.9 bits (294), Expect = 1.4e-26
Identity = 69/221 (31.22%), Postives = 107/221 (48.42%), Query Frame = 0

Query: 17  ISTDPNSKHLGTTVWDASLVFVKFLEK----NCRKGKFSPS------KLKGKRAIELGAG 76
           I  D  S H+GT+VW  SL+  KF E+    +      SP+        + +R IELG G
Sbjct: 20  IQQDNGSMHVGTSVWPCSLILSKFAERWSTLDSSSSTTSPNPYAELFDFRRRRGIELGTG 79

Query: 77  CGLAGFGMALLG-CDVIATDQKEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGN 136
           CG+AG    LLG  +++ TD   V+P L  N++RN  +L +        S++ + + W N
Sbjct: 80  CGVAGMAFYLLGLTEIVLTDIAPVMPALKHNLKRNKTALGK--------SLKTSIVYWNN 139

Query: 137 EDHIKTVGPPFDFIIGTDVVYAEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLE 196
            D I  + PPFD +I  DVVY E  +  L+  +  L      ++LGY+IRS    +   E
Sbjct: 140 RDQISALKPPFDLVIAADVVYIEESVGQLVTAMELLVADDGAVLLGYQIRSPEADKLFWE 199

Query: 197 MWKKNFEVKTVSKSKMDSQYQHPSIQLYIMGLKSAAGNTEN 227
           +    F+++ V    + S Y +    +YI   K      E+
Sbjct: 200 LCDIVFKIEKVPHEHLHSDYAYEETDVYIFRKKVKKNEAES 232

BLAST of Tan0021275 vs. TAIR 10
Match: AT1G73320.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 116.7 bits (291), Expect = 3.1e-26
Identity = 67/193 (34.72%), Postives = 109/193 (56.48%), Query Frame = 0

Query: 27  GTTVWDASLVFVKFLEKNCRKGKFSPSKLKGKRAIELGAGCGLAGFGMALLGCDVIATDQ 86
           G+ +WD+ +V  KFLE +      S   L+GK+ +ELG+GCGL G   ALLG + + TD 
Sbjct: 119 GSVMWDSGVVLGKFLEHSVDSKVLS---LEGKKIVELGSGCGLVGCIAALLGGNAVLTDL 178

Query: 87  KEVLPILTRNIERNTPSLAQMNPSDTFGSIRAAELDWGNEDHIKTVGPPFDFIIGTDVVY 146
            + L +L +NI+ N      ++  +T GS    EL WG++     + P  D+++G+DV+Y
Sbjct: 179 PDRLRLLKKNIQTN------LHRGNTRGSAIVQELVWGDDPDPDLIEPFPDYVLGSDVIY 238

Query: 147 AEHLLEPLLKTIHALAGPKTTIMLGYEIRSTNVHEQMLEMWKKNFEVKTVSKSKMDSQYQ 206
           +E  +  L+KT+  L   +TTI L  E+R+  V E  LE   K+F +  V +++    Y+
Sbjct: 239 SEEAVHHLVKTLLQLCSDQTTIFLSGELRNDAVLEYFLETALKDFAIGRVEQTQWHPDYR 298

Query: 207 HPSIQLYIMGLKS 220
              + LY++  KS
Sbjct: 299 SHRVVLYVLEKKS 302

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q5BLD81.6e-1936.11Protein N-lysine methyltransferase METTL21A OS=Danio rerio OX=7955 GN=mettl21a P... [more]
Q8WXB11.7e-1634.09Protein N-lysine methyltransferase METTL21A OS=Homo sapiens OX=9606 GN=METTL21A ... [more]
A4FV421.9e-1534.43Protein N-lysine methyltransferase METTL21A OS=Bos taurus OX=9913 GN=METTL21A PE... [more]
Q9CQL01.9e-1534.44Protein N-lysine methyltransferase METTL21A OS=Mus musculus OX=10090 GN=Mettl21A... [more]
A4IGU33.2e-1531.35Protein N-lysine methyltransferase METTL21A OS=Xenopus tropicalis OX=8364 GN=met... [more]
Match NameE-valueIdentityDescription
KAG6570813.13.0e-14091.21Protein-lysine methyltransferase METTL21D, partial [Cucurbita argyrosperma subsp... [more]
XP_022944009.14.0e-14090.84protein-lysine methyltransferase METTL21D [Cucurbita moschata] >XP_022944011.1 p... [more]
XP_023512824.18.9e-14090.94protein N-lysine methyltransferase METTL21A [Cucurbita pepo subsp. pepo] >XP_023... [more]
KAG7010654.12.0e-13990.84Protein-lysine methyltransferase METTL21D [Cucurbita argyrosperma subsp. argyros... [more]
XP_022140387.18.3e-13889.45protein N-lysine methyltransferase METTL21A [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1FT981.9e-14090.84protein-lysine methyltransferase METTL21D OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1CGP54.0e-13889.45protein N-lysine methyltransferase METTL21A OS=Momordica charantia OX=3673 GN=LO... [more]
A0A0A0KGI79.9e-13787.54Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G147640 PE=4 SV=1[more]
A0A1S4E5798.4e-13686.83protein N-lysine methyltransferase METTL21A OS=Cucumis melo OX=3656 GN=LOC103502... [more]
A0A6J1JBP53.0e-13387.91protein N-lysine methyltransferase METTL21A OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
Match NameE-valueIdentityDescription
AT1G08125.12.1e-9461.46S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G08125.22.1e-9461.46S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G08125.31.0e-5646.53S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT5G44170.11.4e-2631.22S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
AT1G73320.13.1e-2634.72S-adenosyl-L-methionine-dependent methyltransferases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR019410Lysine methyltransferasePFAMPF10294Methyltransf_16coord: 23..181
e-value: 6.8E-32
score: 110.6
IPR019410Lysine methyltransferasePANTHERPTHR14614HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGENcoord: 15..240
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 4..221
e-value: 1.7E-59
score: 202.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 238..262
NoneNo IPR availablePANTHERPTHR14614:SF98S-ADENOSYL-L-METHIONINE-DEPENDENT METHYLTRANSFERASES SUPERFAMILY PROTEINcoord: 15..240
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 54..193

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0021275.1Tan0021275.1mRNA
Tan0021275.2Tan0021275.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
molecular_function GO:0008168 methyltransferase activity