Tan0020914 (gene) Snake gourd v1

Overview


Name	Tan0020914
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	E3 ubiquitin-protein ligase RHA1B-like
Location	LG06: 64434675 .. 64436182 (+)
RNA-Seq Expression	Tan0020914
Synteny	Tan0020914

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTACAAAAACCCATTTCTAAATTCCTTCATTTCCGATGGGCTTTCCGGTGGGTTGCACGGAGGTTTTCTTTCCCACCATATTTCTTCACTTTCTCTCTCTTCTCGACATCCTGAAACGCCTAATTCTGTCCCTCTTCCGCCTCCTCGGCCTGCCGGAATTTCTCCACTCCGACGCCGCCTTCTCGCCGGAGCTTCGATTCCCCCTGAACCCGCCCGCCTCCGCCGTACTCCTCCGCGAATTCCTCCCCGTGGTCAAATTCTCGGATCTCCCCCAGGCGTCGGAGCCGGCGGAGAGCTGCGCCGTGTGCCTGTACGAGTTCGAGGGGGCGGAGGAGATCCGGTGGTTAACGAACTGCAAGCACATATTCCACAGAGGCTGTGTGGACCGTTGGATGGATCACGATCAGAAGACGTGTCCGCTTTGTAGAACGCCGTTTGTGCCTGATGGGATGATGGACGAGTTCAATCAACGGCTCTGGGCCGCTTCTGGAATTGCTGAGTTTGATTCTGAGTTCAACTCGGTTTTAGGTTAGTGCACCAAAAGAAAAAAAAAAGGGGGAAAGAAATATGCCAATGCCCCTTTATTTAGTGGCTTTTGCCAAATAAATGTTTTTTTTTTTTTTTTTTTTTTGCTTTTTTGGGTTGGGTAGTTGGGAAAAAGGGGTTTTGAAACTGTATATATATATATATATAAATATATATAATGTGTAAATGAGATAGATGAGAATTTGGGCACTTTTGCCCCAAAATGAAAATTTCTTTCTTTCTTTCTTTTAATTATGGATTTTTTTTCCTTTTTGTGTCTGTCTGTCTCTCTCCGTCTTTATGGATTTCAGGAATTGTTCAAATGGGGTAGGATATTGAAATTTTATGTGTGTTTTCTTCTTCTTCTTTTTTTATTTTTTATTTTTTTTAATTATTATTATGGTTTATATTTGGAATGGATGAAGGGTAATAAATGATTTAAAAATTGTTAGATTTTGGTCAATTTTTTTAGTTGAATGATTATAGGTTGGAGATCCAAACATCTATTTTTGAGATGATTGATGTCTTATCTACTAATTAAGCTAAGTTAGATGAGTGTGTCGAAGATCTCATATTGGAAAAGTAGAGAAAAACCTCACAATATATAAGATATATGAGTCACTTCTTTTATTGCCAATTGGTTTTGAGATGGAACCTCATATAATCTAATAGAGTGGTTTTGGTAATTTGTATTGTTTAGGCCCCGTCGGATAATTATTTAGTTTTTTGTTTTTGGTCTTTGAAACTTAAATCTATAAATACTATTTTCTACTAATCAGTGTCTATGTTTTTTATATACTTTCTATCTATGTTTTAAAAAACCAAACTAAGTTTTAAAAACTAAAAAAAGTAATTTTCAAAAATTTGTTTTTATTTTTTAAATTTGGTTAGAAACTTAAATGGTACCTCAAGAAAAATGGAAATCATTATAGAGAAGATGAAGAGAAATTGTGA

mRNA sequence

TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTACAAAAACCCATTTCTAAATTCCTTCATTTCCGATGGGCTTTCCGGTGGGTTGCACGGAGGTTTTCTTTCCCACCATATTTCTTCACTTTCTCTCTCTTCTCGACATCCTGAAACGCCTAATTCTGTCCCTCTTCCGCCTCCTCGGCCTGCCGGAATTTCTCCACTCCGACGCCGCCTTCTCGCCGGAGCTTCGATTCCCCCTGAACCCGCCCGCCTCCGCCGTACTCCTCCGCGAATTCCTCCCCGTGGTCAAATTCTCGGATCTCCCCCAGGCGTCGGAGCCGGCGGAGAGCTGCGCCGTGTGCCTGTACGAGTTCGAGGGGGCGGAGGAGATCCGGTGGTTAACGAACTGCAAGCACATATTCCACAGAGGCTGTGTGGACCGTTGGATGGATCACGATCAGAAGACGTGTCCGCTTTGTAGAACGCCGTTTGTGCCTGATGGGATGATGGACGAGTTCAATCAACGGCTCTGGGCCGCTTCTGGAATTGCTGAGTTTGATTCTGAGTTCAACTCGAAACTTAAATGGTACCTCAAGAAAAATGGAAATCATTATAGAGAAGATGAAGAGAAATTGTGA

Coding sequence (CDS)

ATGGGCTTTCCGGTGGGTTGCACGGAGGTTTTCTTTCCCACCATATTTCTTCACTTTCTCTCTCTTCTCGACATCCTGAAACGCCTAATTCTGTCCCTCTTCCGCCTCCTCGGCCTGCCGGAATTTCTCCACTCCGACGCCGCCTTCTCGCCGGAGCTTCGATTCCCCCTGAACCCGCCCGCCTCCGCCGTACTCCTCCGCGAATTCCTCCCCGTGGTCAAATTCTCGGATCTCCCCCAGGCGTCGGAGCCGGCGGAGAGCTGCGCCGTGTGCCTGTACGAGTTCGAGGGGGCGGAGGAGATCCGGTGGTTAACGAACTGCAAGCACATATTCCACAGAGGCTGTGTGGACCGTTGGATGGATCACGATCAGAAGACGTGTCCGCTTTGTAGAACGCCGTTTGTGCCTGATGGGATGATGGACGAGTTCAATCAACGGCTCTGGGCCGCTTCTGGAATTGCTGAGTTTGATTCTGAGTTCAACTCGAAACTTAAATGGTACCTCAAGAAAAATGGAAATCATTATAGAGAAGATGAAGAGAAATTGTGA

Protein sequence

MGFPVGCTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFLHSDAAFSPELRFPLNPPASAVLLREFLPVVKFSDLPQASEPAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWMDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNSKLKWYLKKNGNHYREDEEKL

Homology

[Close]

BLAST of Tan0020914 vs. ExPASy Swiss-Prot
Match: Q9XF92 (Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH1 PE=2 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 9.1e-53
Identity = 96/162 (59.26%), Postives = 119/162 (73.46%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFLHSDAAFSPELRFP---- 60
           MGFPVG TEVF P +F+  LS+L  ++ ++ S+FR LGL +FL  D  +     +P    
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDYTSYPTRIP 60

Query: 61  -LNPPASAVLLREFLPVVKFSDLPQASEP-AESCAVCLYEFEGAEEIRWLTNCKHIFHRG 120
               P SA+L+RE LPV+KF +L  + E   E+CAVCLYEFEG +EIRWL NC+HIFHR 
Sbjct: 61  ETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRS 120

Query: 121 CVDRWMDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEF 157
           C+DRWMDHDQKTCPLCRTPFVPD M +EFNQRLWAASG+ +F
Sbjct: 121 CLDRWMDHDQKTCPLCRTPFVPDEMQEEFNQRLWAASGVHDF 162

[Close]

BLAST of Tan0020914 vs. ExPASy Swiss-Prot
Match: Q9SUS4 (Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RHA1A PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 7.0e-21
Identity = 61/165 (36.97%), Postives = 94/165 (56.97%), Query Frame = 0


Query: 1   MGFPVG-CTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFLHSDAAFSPEL-RFPLN 60
           MG P    TE+  P+  L  L ++   + ++ +L   +GLP FL  +   +P+L R  L+
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLDHNETSAPDLTRHALS 60

Query: 61  PPASAVLLREFLPVVKFSDLPQASEPAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDR 120
              SA L  E +PVV+FSDLP  ++P + C VCL +FE  +++R L  C H+FH  C+DR
Sbjct: 61  --TSASLANELIPVVRFSDLP--TDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYCLDR 120

Query: 121 W-MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNS 163
           W +D+++  CP+CR  F+P    +++ Q  W  SG   F  E  S
Sbjct: 121 WIVDYNKMKCPVCRHRFLP---KEKYTQSDW-GSGSDWFSDEVES 157

[Close]

BLAST of Tan0020914 vs. ExPASy Swiss-Prot
Match: Q9SUS5 (E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.0e-19
Identity = 56/153 (36.60%), Postives = 85/153 (55.56%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFL-HSDAAFSPELRFPLNP 60
           MG P    E+  P   L  L ++   + ++ +L   +GLP FL H++ + S   R  L+ 
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLDHNETSRSDPTRLALS- 60

Query: 61  PASAVLLREFLPVVKFSDLPQASEPAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120
             SA L  E +PVV+FSDL   ++P + C VCL +F   ++IR L  C H+FH  C+DRW
Sbjct: 61  -TSATLANELIPVVRFSDL--LTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCLDRW 120

Query: 121 -MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAAS 152
            +D ++ TCP+CR  F+P+     F+   W  S
Sbjct: 121 IVDCNKITCPICRNRFLPEEKSTPFD---WGTS 146

[Close]

BLAST of Tan0020914 vs. ExPASy Swiss-Prot
Match: Q9SI09 (Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana OX=3702 GN=XERICO PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.9e-11
Identity = 31/83 (37.35%), Postives = 50/83 (60.24%), Query Frame = 0


Query: 67  REFLPVVKFSDLPQASEPAES-CAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWMDHDQK 126
           R   P ++F  L +  + A++ C+VCL +F+G  EI  L  C H+FH+ C+++W+D+   
Sbjct: 81  RNRTPTLRFESLCRCKKQADNECSVCLSKFQGDSEINKL-KCGHLFHKTCLEKWIDYWNI 140

Query: 127 TCPLCRTPFVPDGMMDEFNQRLW 149
           TCPLCRTP V      + +  +W
Sbjct: 141 TCPLCRTPLVVVPEDHQLSSNVW 162

[Close]

BLAST of Tan0020914 vs. ExPASy Swiss-Prot
Match: Q5XF85 (E3 ubiquitin-protein ligase ATL42 OS=Arabidopsis thaliana OX=3702 GN=ATL42 PE=1 SV=2)

HSP 1 Score: 67.4 bits (163), Expect = 1.9e-10
Identity = 30/64 (46.88%), Postives = 42/64 (65.62%), Query Frame = 0


Query: 68  EFLPVVKFSDLPQASEPAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWMDHDQKTC 127
           E LP+ +FS L + S+    C+VCL +FE  E +R L  C+H FH GC+D+W++    TC
Sbjct: 104 ESLPLFRFSAL-KGSKQGLDCSVCLSKFESVEILRLLPKCRHAFHIGCIDQWLE-QHATC 163

Query: 128 PLCR 132
           PLCR
Sbjct: 164 PLCR 165

[Close]

BLAST of Tan0020914 vs. NCBI nr
Match: XP_023000136.1 (probable E3 ubiquitin-protein ligase RHA1A [Cucurbita maxima])

HSP 1 Score: 318.2 bits (814), Expect = 4.7e-83
Identity = 153/164 (93.29%), Postives = 157/164 (95.73%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFLHSDAAFSPELRFPLNPP 60
           MGFPVGCTEVFFPTIFLH LSLLD+LKRLIL LFRLLGLPEFLHSDAA SPE+ FPLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLHSDAAVSPEVGFPLNPP 60

Query: 61  ASAVLLREFLPVVKFSDLPQASEPAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWM 120
           ASAVLLREFLPVVKFSD+PQA+  AESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWM
Sbjct: 61  ASAVLLREFLPVVKFSDVPQAA--AESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWM 120

Query: 121 DHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNSKL 165
           DHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNS L
Sbjct: 121 DHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNSVL 162

[Close]

BLAST of Tan0020914 vs. NCBI nr
Match: KAG6593657.1 (Brassinosteroid-responsive RING protein 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7025989.1 putative E3 ubiquitin-protein ligase RHA1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 313.2 bits (801), Expect = 1.5e-81
Identity = 151/165 (91.52%), Postives = 156/165 (94.55%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFLHSDAAFSPELRFPLNPP 60
           MGFPVGCTEVFFPTIFLH LSLLD+LKRLIL LFRLLGLPEFLHSDAA S E+  PLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLHSDAAVSREVGIPLNPP 60

Query: 61  ASAVLLREFLPVVKFSDLPQASE-PAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120
           ASAV+LREFLPVVKFSD+PQA+E  AESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW
Sbjct: 61  ASAVVLREFLPVVKFSDVPQAAEAAAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120

Query: 121 MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNSKL 165
           MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNS L
Sbjct: 121 MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNSVL 165

[Close]

BLAST of Tan0020914 vs. NCBI nr
Match: XP_023514786.1 (E3 ubiquitin-protein ligase RHA1B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 310.1 bits (793), Expect = 1.3e-80
Identity = 149/165 (90.30%), Postives = 155/165 (93.94%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFLHSDAAFSPELRFPLNPP 60
           MGFPVGCTEVFFPTIFLH LSLLD+LKRLIL LFRLLGLPEFLHSD+A S E+  PLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLHSDSAISREVGIPLNPP 60

Query: 61  ASAVLLREFLPVVKFSDLPQASE-PAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120
           ASAV+LREFLPVVKFSD+PQA+E  AESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW
Sbjct: 61  ASAVVLREFLPVVKFSDVPQAAEAAAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120

Query: 121 MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNSKL 165
           MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAE DSEFNS L
Sbjct: 121 MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAELDSEFNSVL 165

[Close]

BLAST of Tan0020914 vs. NCBI nr
Match: XP_022964361.1 (E3 ubiquitin-protein ligase RHA1B-like [Cucurbita moschata])

HSP 1 Score: 309.3 bits (791), Expect = 2.2e-80
Identity = 150/165 (90.91%), Postives = 155/165 (93.94%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFLHSDAAFSPELRFPLNPP 60
           MGFPVGCTEVFFPTIFLH LSLLD+LKRLIL LFRLLGLPEFL+SDAA S E   PLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLYSDAAISREGGIPLNPP 60

Query: 61  ASAVLLREFLPVVKFSDLPQASE-PAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120
           ASAV+LREFLPVVKFSD+PQA+E  AESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW
Sbjct: 61  ASAVVLREFLPVVKFSDVPQAAEAAAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120

Query: 121 MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNSKL 165
           MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNS L
Sbjct: 121 MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNSVL 165

[Close]

BLAST of Tan0020914 vs. NCBI nr
Match: XP_038875326.1 (probable E3 ubiquitin-protein ligase RHA1A [Benincasa hispida])

HSP 1 Score: 286.6 bits (732), Expect = 1.5e-73
Identity = 140/165 (84.85%), Postives = 148/165 (89.70%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFLHSDAAFSPELRFPLNPP 60
           MGFPVGCTEVFFPTIFLH LS+LD+LK LI SLFRLLGLPEFLHSD AF PEL+FPLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSVLDVLKLLIQSLFRLLGLPEFLHSD-AFPPELQFPLNPP 60

Query: 61  ASAVLLREFLPVVKFSDLPQASEPAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWM 120
            SA+LLRE LPVVKFSDLP   +P E CAVCLYEFEGAEEIRWLTNCKHIFHR CVDRWM
Sbjct: 61  PSALLLRELLPVVKFSDLP---DPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDRWM 120

Query: 121 DHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAE-FDSEFNSKL 165
           DHD  TCPLCRTPFVPDGMMDEFNQRLWAASGIAE F+++FNS L
Sbjct: 121 DHDHDTCPLCRTPFVPDGMMDEFNQRLWAASGIAETFETDFNSVL 161

[Close]

BLAST of Tan0020914 vs. ExPASy TrEMBL
Match: A0A6J1KF16 (probable E3 ubiquitin-protein ligase RHA1A OS=Cucurbita maxima OX=3661 GN=LOC111494424 PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.3e-83
Identity = 153/164 (93.29%), Postives = 157/164 (95.73%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFLHSDAAFSPELRFPLNPP 60
           MGFPVGCTEVFFPTIFLH LSLLD+LKRLIL LFRLLGLPEFLHSDAA SPE+ FPLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLHSDAAVSPEVGFPLNPP 60

Query: 61  ASAVLLREFLPVVKFSDLPQASEPAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWM 120
           ASAVLLREFLPVVKFSD+PQA+  AESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWM
Sbjct: 61  ASAVLLREFLPVVKFSDVPQAA--AESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWM 120

Query: 121 DHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNSKL 165
           DHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNS L
Sbjct: 121 DHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNSVL 162

[Close]

BLAST of Tan0020914 vs. ExPASy TrEMBL
Match: A0A6J1HIP9 (E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC111464396 PE=4 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.1e-80
Identity = 150/165 (90.91%), Postives = 155/165 (93.94%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFLHSDAAFSPELRFPLNPP 60
           MGFPVGCTEVFFPTIFLH LSLLD+LKRLIL LFRLLGLPEFL+SDAA S E   PLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKRLILYLFRLLGLPEFLYSDAAISREGGIPLNPP 60

Query: 61  ASAVLLREFLPVVKFSDLPQASE-PAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120
           ASAV+LREFLPVVKFSD+PQA+E  AESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW
Sbjct: 61  ASAVVLREFLPVVKFSDVPQAAEAAAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120

Query: 121 MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNSKL 165
           MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNS L
Sbjct: 121 MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNSVL 165

[Close]

BLAST of Tan0020914 vs. ExPASy TrEMBL
Match: A0A5D3BV35 (E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G001290 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 2.0e-71
Identity = 137/165 (83.03%), Postives = 145/165 (87.88%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFLHSDAAFSPELRFPLNPP 60
           MGFPVGCTEVFFPTIFLH LSLLD+LK LILSLFRLLGLPEFL SD    PEL+FPLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSD-NLPPELQFPLNPP 60

Query: 61  ASAVLLREFLPVVKFSDLPQASEPAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWM 120
            SA+LLRE LPVVKFSDLP    P E CAVCLYEFEGAEEIRWLTNCKHIFHR C+DRWM
Sbjct: 61  PSALLLRELLPVVKFSDLP---NPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWM 120

Query: 121 DHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAE-FDSEFNSKL 165
           DHD  TCPLCRTPFVPDGMMDEFNQRLWAASG+AE F+++FNS L
Sbjct: 121 DHDHDTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVL 161

[Close]

BLAST of Tan0020914 vs. ExPASy TrEMBL
Match: A0A1S3C782 (E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103497696 PE=4 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 2.0e-71
Identity = 137/165 (83.03%), Postives = 145/165 (87.88%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFLHSDAAFSPELRFPLNPP 60
           MGFPVGCTEVFFPTIFLH LSLLD+LK LILSLFRLLGLPEFL SD    PEL+FPLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDLLKFLILSLFRLLGLPEFLPSD-NLPPELQFPLNPP 60

Query: 61  ASAVLLREFLPVVKFSDLPQASEPAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWM 120
            SA+LLRE LPVVKFSDLP    P E CAVCLYEFEGAEEIRWLTNCKHIFHR C+DRWM
Sbjct: 61  PSALLLRELLPVVKFSDLP---NPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCLDRWM 120

Query: 121 DHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAE-FDSEFNSKL 165
           DHD  TCPLCRTPFVPDGMMDEFNQRLWAASG+AE F+++FNS L
Sbjct: 121 DHDHDTCPLCRTPFVPDGMMDEFNQRLWAASGVAETFETDFNSVL 161

[Close]

BLAST of Tan0020914 vs. ExPASy TrEMBL
Match: A0A0A0K874 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G006705 PE=4 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 2.2e-70
Identity = 137/165 (83.03%), Postives = 143/165 (86.67%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFLHSDAAFSPELRFPLNPP 60
           MGFPVGCTEVFFPTIFLH LSLLD+LK LILSLFRLLGLPEFL SD    PEL+FPLNPP
Sbjct: 1   MGFPVGCTEVFFPTIFLHLLSLLDVLKFLILSLFRLLGLPEFLPSD-NLPPELQFPLNPP 60

Query: 61  ASAVLLREFLPVVKFSDLPQASEPAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRWM 120
            SA+LLRE LPVVKFSDLP    P E CAVCLYEFEGAEEIRWLTNCKHIFHR CVD WM
Sbjct: 61  PSALLLRELLPVVKFSDLP---NPPERCAVCLYEFEGAEEIRWLTNCKHIFHRRCVDSWM 120

Query: 121 DHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAE-FDSEFNSKL 165
           DHD  TCPLCRTPFVPD MMDEFNQRLWAASGIAE F+++FNS L
Sbjct: 121 DHDHDTCPLCRTPFVPDEMMDEFNQRLWAASGIAETFETDFNSVL 161

[Close]

BLAST of Tan0020914 vs. TAIR 10
Match: AT3G61460.1 (brassinosteroid-responsive RING-H2 )

HSP 1 Score: 208.0 bits (528), Expect = 6.4e-54
Identity = 96/162 (59.26%), Postives = 119/162 (73.46%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFLHSDAAFSPELRFP---- 60
           MGFPVG TEVF P +F+  LS+L  ++ ++ S+FR LGL +FL  D  +     +P    
Sbjct: 1   MGFPVGYTEVFLPKLFVQTLSILGFIRTIVFSIFRFLGLSDFLEMDQTWPDYTSYPTRIP 60

Query: 61  -LNPPASAVLLREFLPVVKFSDLPQASEP-AESCAVCLYEFEGAEEIRWLTNCKHIFHRG 120
               P SA+L+RE LPV+KF +L  + E   E+CAVCLYEFEG +EIRWL NC+HIFHR 
Sbjct: 61  ETRSPFSALLIREILPVIKFEELTNSGEDLPENCAVCLYEFEGEQEIRWLRNCRHIFHRS 120

Query: 121 CVDRWMDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEF 157
           C+DRWMDHDQKTCPLCRTPFVPD M +EFNQRLWAASG+ +F
Sbjct: 121 CLDRWMDHDQKTCPLCRTPFVPDEMQEEFNQRLWAASGVHDF 162

[Close]

BLAST of Tan0020914 vs. TAIR 10
Match: AT1G63840.1 (RING/U-box superfamily protein )

HSP 1 Score: 162.9 bits (411), Expect = 2.4e-40
Identity = 79/155 (50.97%), Postives = 110/155 (70.97%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFLHSDAAFSPELRFP---L 60
           MGFPVG +E+  P IF + LS L ++++LI ++F+++GLP+FL  +   +     P    
Sbjct: 1   MGFPVGYSELLLPKIFFYLLSFLGLIRKLISTMFKIIGLPDFLEPEPVSTSWPDPPPTLT 60

Query: 61  NPPASAVLLREFLPVVKFSDLPQASEPAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVD 120
            P ++A+L  E LPVV+FSD+ +    +E CAVCLY+FE  +EIR LTNC+HIFHRGC+D
Sbjct: 61  KPDSAAILAGEMLPVVRFSDINRPE--SECCAVCLYDFENDDEIRRLTNCRHIFHRGCLD 120

Query: 121 RW-MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAAS 152
           RW M ++Q TCPLCRT F+PD +  EFNQRLW+ S
Sbjct: 121 RWMMGYNQMTCPLCRTQFIPDHLQLEFNQRLWSQS 153

[Close]

BLAST of Tan0020914 vs. TAIR 10
Match: AT5G41400.1 (RING/U-box superfamily protein )

HSP 1 Score: 156.0 bits (393), Expect = 2.9e-38
Identity = 84/169 (49.70%), Postives = 110/169 (65.09%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFLHSD--AAFSPELRFPLN 60
           MG+PVG TE+  P IFLH LSLL +++ LI + FR+LGLP+FL SD  ++ S  L  P  
Sbjct: 1   MGYPVGYTELLLPRIFLHLLSLLGLIRTLIDTGFRILGLPDFLESDPVSSSSSWLEPPYM 60

Query: 61  P-------------PASAVLLREFLPVVKFSDL--PQASEPAESCAVCLYEFEGAEEIRW 120
                         P +A L  E LPV++FS+L  P     ++ CAVCL+EFE  +EIR 
Sbjct: 61  STAAHHHQESSFFFPVAARLAGEILPVIRFSELTRPGFGSGSDCCAVCLHEFENDDEIRR 120

Query: 121 LTNCKHIFHRGCVDRW-MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAAS 152
           LTNC+HIFHR C+DRW M ++Q TCPLCRTPF+ D +   FNQR+W+ S
Sbjct: 121 LTNCQHIFHRSCLDRWMMGYNQMTCPLCRTPFISDELQVAFNQRVWSES 169

[Close]

BLAST of Tan0020914 vs. TAIR 10
Match: AT4G11370.1 (RING-H2 finger A1A )

HSP 1 Score: 102.1 bits (253), Expect = 5.0e-22
Identity = 61/165 (36.97%), Postives = 94/165 (56.97%), Query Frame = 0


Query: 1   MGFPVG-CTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFLHSDAAFSPEL-RFPLN 60
           MG P    TE+  P+  L  L ++   + ++ +L   +GLP FL  +   +P+L R  L+
Sbjct: 1   MGLPEDFITELQIPSYILKILYVIGFFRDIVDALCPYIGLPRFLDHNETSAPDLTRHALS 60

Query: 61  PPASAVLLREFLPVVKFSDLPQASEPAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDR 120
              SA L  E +PVV+FSDLP  ++P + C VCL +FE  +++R L  C H+FH  C+DR
Sbjct: 61  --TSASLANELIPVVRFSDLP--TDPEDCCTVCLSDFESDDKVRQLPKCGHVFHHYCLDR 120

Query: 121 W-MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAASGIAEFDSEFNS 163
           W +D+++  CP+CR  F+P    +++ Q  W  SG   F  E  S
Sbjct: 121 WIVDYNKMKCPVCRHRFLP---KEKYTQSDW-GSGSDWFSDEVES 157

[Close]

BLAST of Tan0020914 vs. TAIR 10
Match: AT4G11360.1 (RING-H2 finger A1B )

HSP 1 Score: 98.2 bits (243), Expect = 7.2e-21
Identity = 56/153 (36.60%), Postives = 85/153 (55.56%), Query Frame = 0


Query: 1   MGFPVGCTEVFFPTIFLHFLSLLDILKRLILSLFRLLGLPEFL-HSDAAFSPELRFPLNP 60
           MG P    E+  P   L  L ++   + ++ +L   +GLP FL H++ + S   R  L+ 
Sbjct: 1   MGLPTDFKELQIPGYVLKTLYVIGFFRDMVDALCPYIGLPSFLDHNETSRSDPTRLALS- 60

Query: 61  PASAVLLREFLPVVKFSDLPQASEPAESCAVCLYEFEGAEEIRWLTNCKHIFHRGCVDRW 120
             SA L  E +PVV+FSDL   ++P + C VCL +F   ++IR L  C H+FH  C+DRW
Sbjct: 61  -TSATLANELIPVVRFSDL--LTDPEDCCTVCLSDFVSDDKIRQLPKCGHVFHHRCLDRW 120

Query: 121 -MDHDQKTCPLCRTPFVPDGMMDEFNQRLWAAS 152
            +D ++ TCP+CR  F+P+     F+   W  S
Sbjct: 121 IVDCNKITCPICRNRFLPEEKSTPFD---WGTS 146

The following BLAST results are available for this feature:

BLAST of Tan0020914 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XF92	9.1e-53	59.26	Brassinosteroid-responsive RING protein 1 OS=Arabidopsis thaliana OX=3702 GN=BRH...	[more]
Q9SUS4	7.0e-21	36.97	Probable E3 ubiquitin-protein ligase RHA1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q9SUS5	1.0e-19	36.60	E3 ubiquitin-protein ligase RHA1B OS=Arabidopsis thaliana OX=3702 GN=RHA1B PE=2 ...	[more]
Q9SI09	2.9e-11	37.35	Probable E3 ubiquitin-protein ligase XERICO OS=Arabidopsis thaliana OX=3702 GN=X...	[more]
Q5XF85	1.9e-10	46.88	E3 ubiquitin-protein ligase ATL42 OS=Arabidopsis thaliana OX=3702 GN=ATL42 PE=1 ...	[more]

BLAST of Tan0020914 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023000136.1	4.7e-83	93.29	probable E3 ubiquitin-protein ligase RHA1A [Cucurbita maxima]	[more]
KAG6593657.1	1.5e-81	91.52	Brassinosteroid-responsive RING protein 1, partial [Cucurbita argyrosperma subsp...	[more]
XP_023514786.1	1.3e-80	90.30	E3 ubiquitin-protein ligase RHA1B-like [Cucurbita pepo subsp. pepo]	[more]
XP_022964361.1	2.2e-80	90.91	E3 ubiquitin-protein ligase RHA1B-like [Cucurbita moschata]	[more]
XP_038875326.1	1.5e-73	84.85	probable E3 ubiquitin-protein ligase RHA1A [Benincasa hispida]	[more]

BLAST of Tan0020914 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KF16	2.3e-83	93.29	probable E3 ubiquitin-protein ligase RHA1A OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1HIP9	1.1e-80	90.91	E3 ubiquitin-protein ligase RHA1B-like OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A5D3BV35	2.0e-71	83.03	E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3C782	2.0e-71	83.03	E3 ubiquitin-protein ligase RHA1B OS=Cucumis melo OX=3656 GN=LOC103497696 PE=4 S...	[more]
A0A0A0K874	2.2e-70	83.03	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G006705 P...	[more]

BLAST of Tan0020914 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G61460.1	6.4e-54	59.26	brassinosteroid-responsive RING-H2	[more]
AT1G63840.1	2.4e-40	50.97	RING/U-box superfamily protein	[more]
AT5G41400.1	2.9e-38	49.70	RING/U-box superfamily protein	[more]
AT4G11370.1	5.0e-22	36.97	RING-H2 finger A1A	[more]
AT4G11360.1	7.2e-21	36.60	RING-H2 finger A1B	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 88..130 e-value: 2.4E-6 score: 37.1
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 87..131 e-value: 3.4E-12 score: 46.5
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 88..131 score: 12.371254
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 58..136 e-value: 2.1E-18 score: 67.8
None	No IPR available	PANTHER	PTHR45969:SF10	BRH1 RING FINGER PROTEIN	coord: 1..159
None	No IPR available	PANTHER	PTHR45969	RING ZINC FINGER PROTEIN-RELATED	coord: 1..159
None	No IPR available	CDD	cd16448	RING-H2	coord: 88..131 e-value: 2.55029E-14 score: 62.0843
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 73..137

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0020914.1	Tan0020914.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:1905421	regulation of plant organ morphogenesis
biological_process	GO:0009741	response to brassinosteroid
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0061630	ubiquitin protein ligase activity