Tan0020887 (gene) Snake gourd v1

BLAST of Tan0020887 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 1.9e-224
Identity = 398/752 (52.93%), Postives = 530/752 (70.48%), Query Frame = 0

Query: 31  ADHDQDRKVHIVYLGERQHDDIKLTTESHHDLLATVLGSKEKSLESMVYSYRHGFSGFAA 90
           A+    RKVHIVYLGE+QHDD +  TESHH +L ++LGSKE + +SMVYSYRHGFSGFAA
Sbjct: 26  AESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAA 85

Query: 91  KLTKSQAQKLAEMPGVVRVLRNSIYQMHTTRSWDFLGLSSSPSSDSDNLLHRGKMGDDII 150
           KLT+SQA+K+A++P VV V+ +S Y++ TTR+WD+LGLS   +++  +LLH   MG+ II
Sbjct: 86  KLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLS---AANPKSLLHETNMGEQII 145

Query: 151 IGVIDSGIWPESEAFSDKGMGPVPSRWRGRCERGEQFNWTNCNRKVIGARWYMEGFIADF 210
           IGVID+G+WPESE F+D G GPVPS W+G CE GE FN +NCN+K+IGA++++ GF+A+ 
Sbjct: 146 IGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKLIGAKYFINGFLAEN 205

Query: 211 GKEALATSKEYSSPRDASGHGTHVASTAAGSFVRNVSYRGHGSGTVRGGAPLARLAIYKA 270
                  S ++ SPRD  GHGTHV++ A GSFV N+SY+G   GTVRGGAP A +A+YKA
Sbjct: 206 ESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAMYKA 265

Query: 271 LWLLNNQ--ASGSSADILMAIDEAISDGVDVLSISIGGSFPLFPEFDEVNEIAIGSFHAI 330
            W L++    + SSADIL A+DEA+ DGVDVLSIS+G S PL+ E D  + I  G+FHA+
Sbjct: 266 CWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGETDIRDGITTGAFHAV 325

Query: 331 ARGISVVCSAGNDGPRQQKVVNTAPWILTVAASTMDRAFLSPITTLGDNTTYFGQSFLNS 390
            +GI+VVCS GN GP    V NTAPWI+TVAA+T+DR+F +P+ TLG+N    GQ+    
Sbjct: 326 LKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPL-TLGNNKVILGQAMYTG 385

Query: 391 KKDLVVQLA---------TSLTGRCDE-IFGNDTSLSGKVVICFSDLADIKTTLAAGAAV 450
                  L           S +G C+E +F ++ ++ GKVV+CF+        L+A   V
Sbjct: 386 PGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKVVLCFTTSPYGGAVLSAARYV 445

Query: 451 IQANGSGIIVAGQNVDILFPCRQSFPCILVDTDVGTKLFFYTLDNRNPMIRLGRAKTIIG 510
            +A G G+I+A      + PC   FPC+ VD ++GT +  YT  + +P++++  +KT++G
Sbjct: 446 KRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKTLVG 505

Query: 511 NPISTKIAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNIFSDKGYATQSGTSMAAP 570
            P+ TK+A FSSRGPNS +PAILKPDIA PG +I+AA +    FSD+G+   SGTSMAAP
Sbjct: 506 QPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAA-TTNTTFSDQGFIMLSGTSMAAP 565

Query: 571 HISGIVALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEGSPPKVADPFDYGGGVVD 630
            ISG+ ALLK++H +WSPAAI+SA++TTA   DP G  IFAEGSPPK+ADPFDYGGG+V+
Sbjct: 566 AISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFAEGSPPKLADPFDYGGGLVN 625

Query: 631 PNAAMDPGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKCPLPRPSILDLNLPTITI 690
           P  + +PGL+YD+   DY+  Y+C +GY  + IS L  + T C  P+PS+LD NLP+ITI
Sbjct: 626 PEKSANPGLVYDMGLEDYV-LYMCSVGYNETSISQLIGKTTVCSNPKPSVLDFNLPSITI 685

Query: 691 PALTNSTTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELAFNSQVKKISFKVTISTV 750
           P L +  T+TRTVTNVG L S+YR  +E P G +VTV P+ L FNS  KK+ FKV +ST 
Sbjct: 686 PNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNSTTKKVYFKVKVSTT 745

Query: 751 LHINYGYSFGSLTWTDGVHLVKTPLSVRIHFL 771
              N GY FGSLTW+D +H V  PLSVR   L
Sbjct: 746 HKTNTGYYFGSLTWSDSLHNVTIPLSVRTQIL 771

BLAST of Tan0020887 vs. ExPASy Swiss-Prot
Match: Q9SZY3 (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 1.2e-223
Identity = 402/747 (53.82%), Postives = 526/747 (70.41%), Query Frame = 0

Query: 36  DRKVHIVYLGERQHDDIKLTTESHHDLLATVLGSKEKSLESMVYSYRHGFSGFAAKLTKS 95
           + KVHIVYLGE+QHDD +  TESHH +L ++LGSKE +  SMV+SYRHGFSGFAAKLTKS
Sbjct: 27  ESKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDAHSSMVHSYRHGFSGFAAKLTKS 86

Query: 96  QAQKLAEMPGVVRVLRNSIYQMHTTRSWDFLGLSSSPSSDSDNLLHRGKMGDDIIIGVID 155
           QA+KLA++P VV V  +S YQ+ TTR+WD+LGLS    ++  NLL+   MG+++IIG++D
Sbjct: 87  QAKKLADLPEVVHVTPDSFYQLDTTRTWDYLGLS---VANPKNLLNDTNMGEEVIIGIVD 146

Query: 156 SGIWPESEAFSDKGMGPVPSRWRGRCERGEQFNWTNCNRKVIGARWYMEGFIADFGKEAL 215
           SG+WPESE F+D G+GPVPS W+G C  GE F  + CN+K+IGA++++ GF+A       
Sbjct: 147 SGVWPESEVFNDNGIGPVPSHWKGGCVSGENFTSSQCNKKLIGAKYFINGFLATHESFNS 206

Query: 216 ATSKEYSSPRDASGHGTHVASTAAGSFVRNVSYRGHGSGTVRGGAPLARLAIYKALWLLN 275
             S ++ SPRD SGHGTHVA+ A GS+V ++SY+G   GTVRGGAP AR+A+YKA W L+
Sbjct: 207 TESLDFISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRGGAPRARIAMYKACWYLD 266

Query: 276 --NQASGSSADILMAIDEAISDGVDVLSISIGGSFPLFPEFDEVNEIAIGSFHAIARGIS 335
             +  + SSADIL A+DEA+ DGVDVLS+SIG  FP FPE D    IA G+FHA+ +GI+
Sbjct: 267 RFDINTCSSADILKAMDEAMHDGVDVLSLSIGYRFPYFPETDVRAVIATGAFHAVLKGIT 326

Query: 336 VVCSAGNDGPRQQKVVNTAPWILTVAASTMDRAFLSPITTLGDNTTYFGQSFLNSKKDLV 395
           VVCS GN GP  Q V NTAPWILTVAA+T+DR+F +PI TLG+N    GQ+     +   
Sbjct: 327 VVCSGGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPI-TLGNNKLILGQAMYTGPELGF 386

Query: 396 VQLA---------TSLTGRCDEIFGNDT-SLSGKVVICFSDLADIKTTLAAGAAVIQANG 455
             L           S +G C+ +F N   +++GKVV+CF+      T  +A + V +A G
Sbjct: 387 TSLVYPENPGNSNESFSGDCELLFFNSNHTMAGKVVLCFTTSTRYITVSSAVSYVKEAGG 446

Query: 456 SGIIVAGQNVDILFPCRQSFPCILVDTDVGTKLFFYTLDNRNPMIRLGRAKTIIGNPIST 515
            G+IVA    D L PC   FPC+ VD ++GT +  Y      P++++  +KT++G P+ T
Sbjct: 447 LGVIVARNPGDNLSPCEDDFPCVAVDYELGTDILLYIRSTGLPVVKIQPSKTLVGQPVGT 506

Query: 516 KIAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNIFSDKGYATQSGTSMAAPHISGI 575
           K+A FSSRGPNS  PAILKPDIA PG +I+AA +    F+D+G+   SGTSMAAP ISG+
Sbjct: 507 KVADFSSRGPNSIEPAILKPDIAAPGVSILAATTTNKTFNDRGFIFLSGTSMAAPTISGV 566

Query: 576 VALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEGSPPKVADPFDYGGGVVDPNAAM 635
           VALLK++H +WSPAAI+SA++TTA   DP G  IFAEGSP K+ADPFDYGGG+V+P  A 
Sbjct: 567 VALLKALHRDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKLADPFDYGGGLVNPEKAA 626

Query: 636 DPGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKCPLPRPSILDLNLPTITIPALTN 695
            PGL+YDL   DY+  Y+C +GY  + IS L  + T C  P+PS+LD NLP+ITIP L +
Sbjct: 627 KPGLVYDLGLEDYV-LYMCSVGYNETSISQLVGKGTVCSNPKPSVLDFNLPSITIPNLKD 686

Query: 696 STTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELAFNSQVKKISFKVTISTVLHINY 755
             T+TRT+TNVG L S+Y+ +IE P G +VTV P+ L FNS  K++SFKV +ST   IN 
Sbjct: 687 EVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVTPETLLFNSTTKRVSFKVKVSTTHKINT 746

Query: 756 GYSFGSLTWTDGVHLVKTPLSVRIHFL 771
           GY FGSLTW+D +H V  PLSVR   L
Sbjct: 747 GYFFGSLTWSDSLHNVTIPLSVRTQIL 768

BLAST of Tan0020887 vs. ExPASy Swiss-Prot
Match: Q9SZY2 (Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 SV=2)

HSP 1 Score: 769.2 bits (1985), Expect = 4.3e-221
Identity = 395/746 (52.95%), Postives = 531/746 (71.18%), Query Frame = 0

Query: 36  DRKVHIVYLGERQHDDIKLTTESHHDLLATVLGSKEKSLESMVYSYRHGFSGFAAKLTKS 95
           + KVHIVYLGE+QHDD +  TESHH +L ++LGSKE++  SMV+S+RHGFSGFAAKLT+S
Sbjct: 31  ESKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEEAHGSMVHSFRHGFSGFAAKLTES 90

Query: 96  QAQKLAEMPGVVRVLRNSIYQMHTTRSWDFLGLSSSPSSDSDNLLHRGKMGDDIIIGVID 155
           QA+K+A++P VV V+ +  Y+  TTR+WD+LGLS    ++  NLL++  MG+ +IIG+ID
Sbjct: 91  QAKKIADLPEVVHVIPDRFYKPATTRTWDYLGLS---PTNPKNLLNQTNMGEQMIIGIID 150

Query: 156 SGIWPESEAFSDKGMGPVPSRWRGRCERGEQFNWTNCNRKVIGARWYMEGFIADFGKEAL 215
           SG+WPESE F+D  +GPVPS W+G CE GE FN ++CN+K+IGA++++  F+A       
Sbjct: 151 SGVWPESEVFNDNEIGPVPSHWKGGCESGEDFNSSHCNKKLIGAKYFINAFLATHESFNS 210

Query: 216 ATSKEYSSPRDASGHGTHVASTAAGSFVRNVSYRGHGSGTVRGGAPLARLAIYKALWLLN 275
           + S ++ SPR  +GHGTHVA+ A GS+V N SY+G   GTVRGGAP AR+A+YK  W L+
Sbjct: 211 SESLDFISPRGYNGHGTHVATIAGGSYVPNTSYKGLAGGTVRGGAPRARIAVYKTCWYLD 270

Query: 276 -NQASGSSADILMAIDEAISDGVDVLSISIGGSFPLFPEFDEVNEIAIGSFHAIARGISV 335
            + A+ SSADIL A+DEAI DGVDVLS+S+G   PL+PE D  + IA G+FHA+ +GI+V
Sbjct: 271 LDIAACSSADILKAMDEAIHDGVDVLSLSLGFE-PLYPETDVRDGIATGAFHAVLKGITV 330

Query: 336 VCSAGNDGPRQQKVVNTAPWILTVAASTMDRAFLSPITTLGDNTTYFGQSFLNSKKDLVV 395
           VC+AGN GP  Q V NTAPWILTVAA+T+DR+F++P+ TLG+N    GQ+     +    
Sbjct: 331 VCAAGNAGPAAQTVGNTAPWILTVAATTLDRSFVTPM-TLGNNKVILGQAIYTGTEVGFT 390

Query: 396 QLA---------TSLTGRCDEIFGN-DTSLSGKVVICFSDLADIKTTLAAGAAVIQANGS 455
            L           S +G C+ +  N + +++GKVV+CF++     +   A   V +A G 
Sbjct: 391 SLVYPENPGNSNESFSGTCERLLINSNRTMAGKVVLCFTESPYSISVTRAAHYVKRAGGL 450

Query: 456 GIIVAGQNVDILFPCRQSFPCILVDTDVGTKLFFYTLDNRNPMIRLGRAKTIIGNPISTK 515
           G+I+AGQ  ++L PC   FPC+ VD ++GT + FY   N +P++++  ++T+IG P+ TK
Sbjct: 451 GVIIAGQPGNVLRPCLDDFPCVAVDYELGTYILFYIRSNGSPVVKIQPSRTLIGQPVGTK 510

Query: 516 IAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNIFSDKGYATQSGTSMAAPHISGIV 575
           +A FSSRGPN  S AILKPDIA PG +I+AA +    F+D+G+   SGTSMA P ISGIV
Sbjct: 511 VASFSSRGPNPISAAILKPDIAAPGVSILAATTTNTTFNDRGFIFLSGTSMATPTISGIV 570

Query: 576 ALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEGSPPKVADPFDYGGGVVDPNAAMD 635
           ALLK++HP+WSPAAI+SA++TTA   DP G  IFAEGSP K ADPFDYGGG+V+P  A  
Sbjct: 571 ALLKALHPDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKPADPFDYGGGLVNPEKATK 630

Query: 636 PGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKCPLPRPSILDLNLPTITIPALTNS 695
           PGL+YDL   DY+  Y+C +GY  + IS L  + T C  P+PS+LD NLP+ITIP L   
Sbjct: 631 PGLVYDLGLEDYV-LYMCSVGYNETSISQLVGKGTVCSYPKPSVLDFNLPSITIPNLKEE 690

Query: 696 TTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELAFNSQVKKISFKVTISTVLHINYG 755
            T+ RT+TNVG L S+YR  +E P GT+VTV P+ L FNS  K++SFKV++ST   IN G
Sbjct: 691 VTLPRTLTNVGPLESVYRVAVEPPLGTQVTVTPETLVFNSTTKRVSFKVSVSTTHKINTG 750

Query: 756 YSFGSLTWTDGVHLVKTPLSVRIHFL 771
           Y FGSLTW+D +H V  PLSVR   L
Sbjct: 751 YYFGSLTWSDSLHNVTIPLSVRTQLL 770

BLAST of Tan0020887 vs. ExPASy Swiss-Prot
Match: F4HPF1 (Subtilisin-like protease SBT3.4 OS=Arabidopsis thaliana OX=3702 GN=SBT3.4 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 5.6e-221
Identity = 401/746 (53.75%), Postives = 523/746 (70.11%), Query Frame = 0

Query: 38  KVHIVYLGERQHDDIKLTTESHHDLLATVLGSKEKSLESMVYSYRHGFSGFAAKLTKSQA 97
           KVHIVYLGE+QHDD K  TESHH +L+++LGSK+ + ESMVYSYRHGFSGFAAKLTKSQA
Sbjct: 28  KVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKLTKSQA 87

Query: 98  QKLAEMPGVVRVLRNSIYQMHTTRSWDFLGLSSSPSSD-SDNLLHRGKMGDDIIIGVIDS 157
           +K+A+ P V+ V+ +S Y++ TTR WD+LG    PS+D S NL+    MGD  IIGVID+
Sbjct: 88  KKIADSPEVIHVIPDSYYELATTRIWDYLG----PSADNSKNLVSDTNMGDQTIIGVIDT 147

Query: 158 GIWPESEAFSDKGMGPVPSRWRGRCERGEQFNWTNCNRKVIGARWYMEGFIADFGKEALA 217
           G+WPESE+F+D G+GPVPS W+G CE GE F  TNCNRK+IGA++++ GF+A+    A  
Sbjct: 148 GVWPESESFNDYGVGPVPSHWKGGCEPGENFISTNCNRKLIGAKYFINGFLAENQFNA-T 207

Query: 218 TSKEYSSPRDASGHGTHVASTAAGSFVRNVSYRGHGSGTVRGGAPLARLAIYKALWLLN- 277
            S +Y S RD  GHGTHVAS A GSFV NVSY+G G GT+RGGAP AR+A+YKA W +N 
Sbjct: 208 ESPDYISARDFDGHGTHVASIAGGSFVPNVSYKGLGRGTLRGGAPRARIAMYKACWYINE 267

Query: 278 -NQASGSSADILMAIDEAISDGVDVLSISIGGSFPLFPEFDEVNEIAIGSFHAIARGISV 337
            +  + S +DI+ AIDEAI DGVDVLSIS+GG  PL  E D  + IA G+FHA+A+GI V
Sbjct: 268 LDGVTCSFSDIMKAIDEAIHDGVDVLSISLGGRVPLNSETDLRDGIATGAFHAVAKGIVV 327

Query: 338 VCSAGNDGPRQQKVVNTAPWILTVAASTMDRAFLSPITTLGDNTTYFGQSFLNSKKDLVV 397
           VC+ GN GP  Q VVNTAPWILTVAA+T+DR+F +PI  LG+N    GQ+     +    
Sbjct: 328 VCAGGNAGPSSQTVVNTAPWILTVAATTLDRSFATPI-ILGNNQVILGQAMYIGPELGFT 387

Query: 398 QLA---------TSLTGRCDEI-FGNDTSLSGKVVICFSDLADIKTTLAAGAAVIQANGS 457
            L           + +G C+ +   ++ +++GKVV+CF+   D      A + V  A G 
Sbjct: 388 SLVYPEDPGNSIDTFSGVCESLNLNSNRTMAGKVVLCFTTARDFTVVSTAASIVKAAGGL 447

Query: 458 GIIVAGQNVDILFPCRQSFPCILVDTDVGTKLFFYTLDNRNPMIRLGRAKTIIGNPISTK 517
           G+I+A      L PC   FPC+ +D ++GT + FY     +P++++  ++T++G P+ TK
Sbjct: 448 GLIIARNPGYNLAPCSDDFPCVAIDNELGTDILFYIRYTGSPVVKIQPSRTLVGEPVGTK 507

Query: 518 IAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNIFSDKGYATQSGTSMAAPHISGIV 577
           +A FSSRGPNS SPAILKPDIA PG +I+AA SP +  +  G+  +SGTSMAAP ISG++
Sbjct: 508 VATFSSRGPNSISPAILKPDIAAPGVSILAATSPNDTLNAGGFVMRSGTSMAAPVISGVI 567

Query: 578 ALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEGSPPKVADPFDYGGGVVDPNAAMD 637
           ALLKS+HP+WSPAA +SA++TTA   DP G  I AE S  KV DPFDYGGG+V+P  A +
Sbjct: 568 ALLKSLHPDWSPAAFRSAIVTTAWRTDPFGEQIAAESSSLKVPDPFDYGGGLVNPEKAAE 627

Query: 638 PGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKCPLPRPSILDLNLPTITIPALTNS 697
           PGLI D+D+ DY+  YLC  GY  S IS L  + T C  P+PS+LD+NLP+ITIP L + 
Sbjct: 628 PGLILDMDSQDYV-LYLCSAGYNDSSISRLVGKVTVCSNPKPSVLDINLPSITIPNLKDE 687

Query: 698 TTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELAFNSQVKKISFKVTISTVLHINYG 757
            T+TRTVTNVG + S+Y+ L+E P G +V V P+ L FNS+ K +SF V +ST   IN G
Sbjct: 688 VTLTRTVTNVGPVDSVYKVLVEPPLGIQVVVTPETLVFNSKTKSVSFTVIVSTTHKINTG 747

Query: 758 YSFGSLTWTDGVHLVKTPLSVRIHFL 771
           + FGSLTWTD +H V  P+SVR   L
Sbjct: 748 FYFGSLTWTDSIHNVVIPVSVRTQIL 766

BLAST of Tan0020887 vs. ExPASy Swiss-Prot
Match: Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 765.0 bits (1974), Expect = 8.1e-220
Identity = 396/740 (53.51%), Postives = 531/740 (71.76%), Query Frame = 0

Query: 36  DRKVHIVYLGERQHDDIKLTTESHHDLLATVLGSKEKSLESMVYSYRHGFSGFAAKLTKS 95
           + KV++VYLGE++HD+ +  TESHH +L ++LGSKE  L+S+VYSYRHGFSGFAAKLT+S
Sbjct: 26  ESKVYVVYLGEKEHDNPESVTESHHQMLWSLLGSKEAVLDSIVYSYRHGFSGFAAKLTES 85

Query: 96  QAQKLAEMPGVVRVLRNSIYQMHTTRSWDFLGLSSSPSSDSDNLLHRGKMGDDIIIGVID 155
           QAQ+++E+P VV+V+ N++Y+M TTR+WD+LG+S     +SD+LL +  MG ++I+GVID
Sbjct: 86  QAQQISELPEVVQVIPNTLYEMTTTRTWDYLGVS---PGNSDSLLQKANMGYNVIVGVID 145

Query: 156 SGIWPESEAFSDKGMGPVPSRWRGRCERGEQFNWT-NCNRKVIGARWYMEGFIADFGKEA 215
           SG+WPESE F+DKG GP+PSRW+G CE GE FN + +CNRK+IGA+++++G +A+FG   
Sbjct: 146 SGVWPESEMFNDKGFGPIPSRWKGGCESGELFNASIHCNRKLIGAKYFVDGLVAEFGVVN 205

Query: 216 LATSKEYSSPRDASGHGTHVASTAAGSFVRNVSYRGHGSGTVRGGAPLARLAIYKALWLL 275
              + EY SPRD +GHGTHVAST  GSF+ NVSY G G GT RGGAP   +A+YKA W  
Sbjct: 206 RTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVSYVGLGRGTARGGAPGVHIAVYKACW-- 265

Query: 276 NNQASGSSADILMAIDEAISDGVDVLSISIGGSFPLFPEFDEVNEIAIGSFHAIARGISV 335
                 S AD+L A+DEAI DGVD+LS+S+G S PLFPE +     ++G+FHA+A+GI V
Sbjct: 266 --SGYCSGADVLKAMDEAIHDGVDILSLSLGPSVPLFPETEHT---SVGAFHAVAKGIPV 325

Query: 336 VCSAGNDGPRQQKVVNTAPWILTVAASTMDRAFLSPITTLGDNTTYFGQSFLNSKKDLVV 395
           V +AGN GP  Q + N APW+LTVAA+T DR+F + I TLG+N T  GQ+     +   V
Sbjct: 326 VIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFPTAI-TLGNNITILGQAIYGGPELGFV 385

Query: 396 QLA---TSLTGRCDEIFGN-DTSLSGKVVICFSDLADIKTTLAAGAAVIQANGSGIIVAG 455
            L    + L+G C+++  N ++++ GKVV+CF   A    + AA AAVI A G G+I+A 
Sbjct: 386 GLTYPESPLSGDCEKLSANPNSTMEGKVVLCF---AASTPSNAAIAAVINAGGLGLIMAK 445

Query: 456 QNVDILFPCRQSFPCILVDTDVGTKLFFYTLDNRNPMIRLGRAKTIIGNPISTKIAHFSS 515
                L P R+ FP + +D ++GT + FY    R+P++++  +KT+ G  +STK+A FSS
Sbjct: 446 NPTHSLTPTRK-FPWVSIDFELGTDILFYIRSTRSPIVKIQASKTLFGQSVSTKVATFSS 505

Query: 516 RGPNSASPAILKPDIAGPGSNIVAAISPRNIFSDKGYATQSGTSMAAPHISGIVALLKSV 575
           RGPNS SPAILKPDIA PG NI+AAISP +  +D G+A  SGTSMA P +SG+V LLKS+
Sbjct: 506 RGPNSVSPAILKPDIAAPGVNILAAISPNSSINDGGFAMMSGTSMATPVVSGVVVLLKSL 565

Query: 576 HPNWSPAAIKSALITTARAKDPSGLPIFAEGSPPKVADPFDYGGGVVDPNAAMDPGLIYD 635
           HP+WSP+AIKSA++TTA   DPSG PIFA+GS  K+ADPFDYGGG+++P  A+ PGLIYD
Sbjct: 566 HPDWSPSAIKSAIVTTAWRTDPSGEPIFADGSSRKLADPFDYGGGLINPEKAVKPGLIYD 625

Query: 636 LDTTDYIHYYLCGMGYKLSDISLLTHRKTKCPLPRPSILDLNLPTITIPALTNSTTVTRT 695
           + T DY+  Y+C + Y    IS +  + T CP P+PS+LDLNLP+ITIP L    T+TRT
Sbjct: 626 MTTDDYV-MYMCSVDYSDISISRVLGKITVCPNPKPSVLDLNLPSITIPNLRGEVTLTRT 685

Query: 696 VTNVGNLTSIYRALIEAPPGTKVTVDPQELAFNSQVKKISFKVTISTVLHINYGYSFGSL 755
           VTNVG + S+Y+ +I+ P G  V V P EL F+    K SF V +ST   +N GY FGSL
Sbjct: 686 VTNVGPVNSVYKVVIDPPTGINVAVTPAELVFDYTTTKRSFTVRVSTTHKVNTGYYFGSL 745

Query: 756 TWTDGVHLVKTPLSVRIHFL 771
           TWTD +H V  P+SVR   L
Sbjct: 746 TWTDNMHNVAIPVSVRTQIL 749

BLAST of Tan0020887 vs. NCBI nr
Match: XP_004146562.1 (subtilisin-like protease SBT3.6 [Cucumis sativus] >KGN53334.1 hypothetical protein Csa_014762 [Cucumis sativus])

HSP 1 Score: 1021.1 bits (2639), Expect = 4.8e-294
Identity = 522/764 (68.32%), Postives = 617/764 (80.76%), Query Frame = 0

Query: 9   IVAVMTFYALFSMFAYKPMAEEADHDQDRKVHIVYLGERQHDDIKLTTESHHDLLATVLG 68
           IV  + F  LF     KP+  EAD DQ+ KVHIVYLGE+ H D K T +SHH LL+T+LG
Sbjct: 11  IVGFIIFDCLF-----KPILAEAD-DQNPKVHIVYLGEKPHHDTKFTIDSHHQLLSTILG 70

Query: 69  SKEKSLESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLRNSIYQMHTTRSWDFLGL 128
           SKEKS+E+MVYSY+HGFSGFAAKLTKSQAQKL+EM  VVRV+ +S+Y++HTTRSWDFLGL
Sbjct: 71  SKEKSMEAMVYSYKHGFSGFAAKLTKSQAQKLSEMSRVVRVVPSSLYKVHTTRSWDFLGL 130

Query: 129 SSSPSSDSDNLLHRGKMGDDIIIGVIDSGIWPESEAFSDKGMGPVPSRWRGRCERGEQFN 188
           SSSP  +S NLLHR +MG+++IIGVID+GIWPESE+F DKG+G +PSRW+G CE GEQFN
Sbjct: 131 SSSP-FESSNLLHRAQMGENVIIGVIDTGIWPESESFKDKGVGSIPSRWKGTCESGEQFN 190

Query: 189 WTNCNRKVIGARWYMEGFIADFGKEALATSKEYSSPRDASGHGTHVASTAAGSFVRNVSY 248
            TNCN+K+IGARW+M+GF+AD G++ALA  KEY SPRD +GHGTH AS AAGSFV N++Y
Sbjct: 191 STNCNKKIIGARWFMKGFVADLGRDALA--KEYLSPRDLNGHGTHTASIAAGSFVANINY 250

Query: 249 RGHGSGTVRGGAPLARLAIYKALWLLNNQASGSSADILMAIDEAISDGVDVLSISIGGSF 308
             + +GTVRGGAPLARLAIYKALW     A GS+ADIL AIDEAI+DGVDVLS+SIG   
Sbjct: 251 HNNAAGTVRGGAPLARLAIYKALW--TKDAVGSTADILKAIDEAINDGVDVLSMSIGSLT 310

Query: 309 PLFPEFDEVNEIAIGSFHAIARGISVVCSAGNDGPRQQKVVNTAPWILTVAASTMDRAFL 368
           P  PEF+E N+IA GSFHAIA+GISVVC+AGN GP  Q V N APWI TVAA+T+DRAFL
Sbjct: 311 PFLPEFNEANDIAFGSFHAIAKGISVVCAAGNSGPTPQTVENVAPWIFTVAANTIDRAFL 370

Query: 369 SPITTLGDNTTYFGQSFLNSKKDLVVQLATSLTGRCDEIFGNDTSLSGKVVICFSDLADI 428
           + ITTL DNTT+ GQS L+SKKDLV +L T  TGRCD++ GN+T ++GKVV+CFS+LAD 
Sbjct: 371 ASITTLPDNTTFLGQSLLDSKKDLVAELETLDTGRCDDLLGNETFINGKVVMCFSNLADH 430

Query: 429 KTTLAAGAAVIQANGSGIIVAGQNVDILFPCRQS-FPCILVDTDVGTKLFFYTL--DNRN 488
            T   A  AV +ANG+GIIVAGQ  D LF C  S  PCILVDTDVG+KLFF  L  ++ N
Sbjct: 431 NTIYDAAMAVARANGTGIIVAGQQDDDLFSCIPSPIPCILVDTDVGSKLFFINLLQNSTN 490

Query: 489 PMIRLGRAKTIIGNPISTKIAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNIFSDK 548
           P++RL   +TIIG PI+  I++FSSRGPNS S  ILKPDI+ PGSNI+AA+SP +IF++K
Sbjct: 491 PVVRLRATRTIIGKPITPAISYFSSRGPNSVSNPILKPDISAPGSNILAAVSPHHIFNEK 550

Query: 549 GYATQSGTSMAAPHISGIVALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEGSPPK 608
           G+   SGTSMA PHIS IVALLKSVHP WSPAAIKSAL+TTAR +   GLPIFAEG+PPK
Sbjct: 551 GFMLLSGTSMATPHISAIVALLKSVHPTWSPAAIKSALMTTARTEVSPGLPIFAEGTPPK 610

Query: 609 VADPFDYGGGVVDPNAAMDPGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKCPLPR 668
           +ADPFDYGGG+VD NAA+DPGL+YD+   DYI YYLCGMGYK  DIS LT RKT CPL R
Sbjct: 611 MADPFDYGGGIVDANAAVDPGLVYDMGRKDYIDYYLCGMGYKDEDISHLTQRKTVCPLQR 670

Query: 669 PSILDLNLPTITIPALTNSTTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELAFNSQ 728
            S+LDLNLP ITIP+L NST VTRTVTNVGNL+ +Y+A IE+P G KV+V+PQ L FNSQ
Sbjct: 671 LSVLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAEIESPFGCKVSVNPQVLVFNSQ 730

Query: 729 VKKISFKVTISTVLHINYGYSFGSLTWTDGVHLVKTPLSVRIHF 770
           VKKISFKV   T +  NYGYSFG LTWTDG+H+VK PLSVR  F
Sbjct: 731 VKKISFKVMFFTQVQRNYGYSFGRLTWTDGIHVVKIPLSVRFGF 763

BLAST of Tan0020887 vs. NCBI nr
Match: XP_008452040.1 (PREDICTED: subtilisin-like protease SBT3.7 isoform X1 [Cucumis melo])

HSP 1 Score: 1011.5 bits (2614), Expect = 3.8e-291
Identity = 523/767 (68.19%), Postives = 613/767 (79.92%), Query Frame = 0

Query: 6   ASFIVAVMTFYALFSMFAYKPMAEEADHDQDRKVHIVYLGERQHDDIKLTTESHHDLLAT 65
           A  IV  + FY L      +P+  E+ +DQ+ KVHIVYLGE+ H D K TT+SHH LLA 
Sbjct: 8   AHLIVGFIIFYGL-----CEPILAES-NDQNPKVHIVYLGEKPHHDTKFTTDSHHQLLAA 67

Query: 66  VLGSKEKSLESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLRNSIYQMHTTRSWDF 125
           +LGSKEKSLE+MVYSY+HGFSGFAAKLTKS+AQKL+EM  VVRV+ +S+Y++HTTRSWDF
Sbjct: 68  ILGSKEKSLEAMVYSYKHGFSGFAAKLTKSEAQKLSEMSRVVRVVPSSLYKVHTTRSWDF 127

Query: 126 LGLSSSPSSDSDNLLHRGKMGDDIIIGVIDSGIWPESEAFSDKGMGPVPSRWRGRCERGE 185
           LGLSSSP S+S NLLHR K GDD+IIGVIDSGIWPESE+F DKG+GP+PSRW+G CE GE
Sbjct: 128 LGLSSSP-SESSNLLHRAK-GDDVIIGVIDSGIWPESESFKDKGLGPIPSRWKGTCESGE 187

Query: 186 QFNWTNCNRKVIGARWYMEGFIADFGKEALATSKEYSSPRDASGHGTHVASTAAGSFVRN 245
           QFN TNCN+K+IGARW+++ F+AD+G+EALA  KEY SPRD  GHGTH ASTAAGSFV N
Sbjct: 188 QFNSTNCNKKIIGARWFVKAFVADYGREALA--KEYLSPRDLHGHGTHTASTAAGSFVAN 247

Query: 246 VSYRGHGSGTVRGGAPLARLAIYKALWLLNNQASGSSADILMAIDEAISDGVDVLSISIG 305
           ++Y  + +GT RGGAPLARLAIYKALW   N+  GSSADIL AIDEAI DGVDVLSISIG
Sbjct: 248 INYHNNAAGTARGGAPLARLAIYKALW--TNRGVGSSADILKAIDEAIHDGVDVLSISIG 307

Query: 306 GSFPLFPEFDEVNEIAIGSFHAIARGISVVCSAGNDGPRQQKVVNTAPWILTVAASTMDR 365
           GS P +PEF E+++IA GSFHAI +GISVVC+AGN GP  Q V N APWI TVA +T+DR
Sbjct: 308 GSPPFYPEFTELSDIAFGSFHAITKGISVVCAAGNSGPSPQMVDNVAPWIFTVATNTIDR 367

Query: 366 AFLSPITTLGDNTTYFGQSFLNSKKDLVVQLATSLTGRCDEIFGNDTSLSGKVVICFSDL 425
           AFLS ITTL DNTT+ GQS L SKKDLV +L +    RCD++ GN+  ++GKVV+CF  L
Sbjct: 368 AFLSSITTLRDNTTFMGQSLLESKKDLVAELVS--WERCDQLSGNEAFINGKVVLCFPKL 427

Query: 426 ADIKTTLAAGAAVIQANGSGIIVAGQNVD-ILFPCRQS-FPCILVDTDVGTKLFFYTLDN 485
           AD  T   A   V++ANG+GIIVAGQ VD  L  C  S  PCILVDT VG+KLFFY L N
Sbjct: 428 ADYSTISKAAEVVVRANGTGIIVAGQQVDNNLLACISSPIPCILVDTIVGSKLFFYFLQN 487

Query: 486 R-NPMIRLGRAKTIIGNPISTKIAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNIF 545
             +P++ L  A+TIIG PI+  IAHFSSRGPNS SP ILKPDI+ PGSNI++A+SP   F
Sbjct: 488 SDHPVVMLRAARTIIGKPIAPTIAHFSSRGPNSVSPPILKPDISAPGSNILSAVSPHYFF 547

Query: 546 SDKGYATQSGTSMAAPHISGIVALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEGS 605
           ++KG++  SGTSMA PH+S IVALLKSVHP WSPAAIKSAL+TTAR +   GLPIFA+G+
Sbjct: 548 NEKGFSLMSGTSMATPHVSAIVALLKSVHPTWSPAAIKSALMTTARTEVSPGLPIFADGT 607

Query: 606 PPKVADPFDYGGGVVDPNAAMDPGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKCP 665
           PPKVADPFDYG GVVD NAA+DPGLIYD+   DYI YYLCGMGY+  DIS LTHRKT+CP
Sbjct: 608 PPKVADPFDYGAGVVDANAAVDPGLIYDMGRKDYIDYYLCGMGYEDKDISHLTHRKTECP 667

Query: 666 LPRPSILDLNLPTITIPALTNSTTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELAF 725
           L R S+LDLNLP ITIP+L NST VTRTVTNVGNL+ +Y+A IEAP G KV+V+PQ L F
Sbjct: 668 LQRLSLLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAKIEAPFGCKVSVNPQVLVF 727

Query: 726 NSQVKKISFKVTISTVLHINYGYSFGSLTWTDGVHLVKTPLSVRIHF 770
           NSQVKKISFKV   T +  NYGYSFG LTW+DGVH+VK PLSVR  F
Sbjct: 728 NSQVKKISFKVMFFTQVQRNYGYSFGRLTWSDGVHVVKIPLSVRFGF 760

BLAST of Tan0020887 vs. NCBI nr
Match: XP_022984814.1 (subtilisin-like protease SBT3.9 [Cucurbita maxima])

HSP 1 Score: 1001.9 bits (2589), Expect = 3.0e-288
Identity = 525/766 (68.54%), Postives = 611/766 (79.77%), Query Frame = 0

Query: 7   SFIVAVMTFYALFSMFAYKPMAEEADHDQDRKVHIVYLGERQHDDIKLTTESHHDLLATV 66
           SFI+AV+T YA+FS  A      EAD DQ+ KVHIVYLGER +DD+KLTT+SHH+LL +V
Sbjct: 8   SFILAVVTIYAVFSDMA------EAD-DQNSKVHIVYLGERPYDDVKLTTDSHHELLESV 67

Query: 67  LGSKEKSLESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLRNSIYQMHTTRSWDFL 126
           LGSKEKSLESMVYSYRHGFSGFAAKLT SQAQKLA MP V RV  +S+Y+M TTRSWDFL
Sbjct: 68  LGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTRSWDFL 127

Query: 127 GLSSSPSSDSDNLLHRGKMGDDIIIGVIDSGIWPESEAFSDKGMGPVPSRWRGRCERGEQ 186
           GLSSSP S+S NL HR KMGD++IIGVID+G WPESE+F+DKGMGP+PSRW+G C+ GE 
Sbjct: 128 GLSSSP-SESSNLFHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGED 187

Query: 187 FNWTNCNRKVIGARWYMEGFIADFGKEALATSKEYSSPRDASGHGTHVASTAAGSFVRNV 246
           FN ++CN+KVIGARW+    IAD G+EA+   K+Y S RD  GHGTH ASTA G+FVRNV
Sbjct: 188 FNSSHCNKKVIGARWFASALIADHGEEAV--FKDYLSARDNEGHGTHTASTAGGAFVRNV 247

Query: 247 SYRGHGSGTVRGGAPLARLAIYKALWLLNNQASGSSADILMAIDEAISDGVDVLSISIGG 306
           SY G+G GT+RGGAPLARLAIYK LW  ++   GS ADIL  IDEAI DGVDVLS+SIG 
Sbjct: 248 SYFGNGRGTLRGGAPLARLAIYKVLW--SDSRLGSGADILKGIDEAIHDGVDVLSMSIGK 307

Query: 307 SFPLFPEFDEVNEIAIGSFHAIARGISVVCSAGNDGPRQQKVVNTAPWILTVAASTMDRA 366
           S PLFP+ ++VN +A+GSFHAIA+GISVVC+ GN+G  QQ V N APW+ TVAAST+DRA
Sbjct: 308 SIPLFPDVNDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRA 367

Query: 367 FLSPITTLGDNTTYFGQSFLNSKKDLVVQLATSLTGRCDEIFGNDTSLSGKVV-ICFSDL 426
           FL  ITTLGDN TY GQ FL  +KD+V  L  ++ GRC  I G D  +SG VV +CF+DL
Sbjct: 368 FLVSITTLGDNATYLGQMFL--RKDIVGML-VAMDGRCAGILGGDIPISGNVVLLCFTDL 427

Query: 427 ADIKTTLAAGAAV--IQANGSGIIVAGQNVDILFPCRQSFPCILVDTDVGTKLFFYTLDN 486
           A  KT  A+ A +   QA   G+I AGQ  D L PC    PCI VDT VGTKLF Y L++
Sbjct: 428 A--KTAPASNAVMPGKQAKVVGVIYAGQQTDNLVPC--DIPCIHVDTHVGTKLFTYLLND 487

Query: 487 RNPMIRLGRAKTIIGNPISTKIAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNIFS 546
            + +IRL   +TIIG PIS++IA+FSSRGPNS SP ILKPDIA PG+NI+AA+ P +   
Sbjct: 488 DDALIRLRATRTIIGKPISSRIAYFSSRGPNSFSPGILKPDIAAPGANIIAAVPPNHKGG 547

Query: 547 DKGYATQSGTSMAAPHISGIVALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEGSP 606
           DKG+AT SGTSMA PHISGIV L+KS+HP WSPAAIKSALITTAR +DPSG+PIFAEGSP
Sbjct: 548 DKGFATMSGTSMATPHISGIVTLIKSLHPTWSPAAIKSALITTARVEDPSGMPIFAEGSP 607

Query: 607 PKVADPFDYGGGVVDPNAAMDPGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKCPL 666
           PKVADPFDYGGGVVD NAA+DPGLIYDL  TDYI+YYLC MGY  +DIS L+ +KT CP 
Sbjct: 608 PKVADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPS 667

Query: 667 PRPSILDLNLPTITIPALTNSTTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELAFN 726
            R SILDLNLPTIT+PAL NSTTVTRTVTNVGNLT++Y+A+I+APPG+KV V P+ L FN
Sbjct: 668 KRASILDLNLPTITVPALINSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFN 727

Query: 727 SQVKKISFKVTISTVLHINYGYSFGSLTWTDGVHLVKTPLSVRIHF 770
           S VKKISFKV  S+ L  NYGYSFGSLTWTDGVHLVK+PLSVR  F
Sbjct: 728 SNVKKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDF 754

BLAST of Tan0020887 vs. NCBI nr
Match: XP_023552781.1 (subtilisin-like protease SBT3.7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 987.3 bits (2551), Expect = 7.7e-284
Identity = 520/767 (67.80%), Postives = 609/767 (79.40%), Query Frame = 0

Query: 9   IVAVMTFYALFSMFAYKPMAEEADHDQDRKVHIVYLGERQHDDIKLTTESHHDLLATVLG 68
           +VAV+T Y +FS  A      EAD DQ+ KVHIVYLGER +DD+KLTT+SHH+LL +VLG
Sbjct: 12  VVAVVTIYGVFSAMA------EAD-DQNSKVHIVYLGERPYDDVKLTTDSHHELLESVLG 71

Query: 69  SKEKSLESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLRNSIYQMHTTRSWDFLGL 128
           SKEKSLES+VYSYRHGFSGFAAKLT SQAQKLA MP V RV  + +Y+M TTRSWDFLGL
Sbjct: 72  SKEKSLESIVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSPLYKMRTTRSWDFLGL 131

Query: 129 SSSPSSDSDNLLHRGKMGDDIIIGVIDSGIWPESEAFSDKGMGPVPSRWRGRCERGEQFN 188
           SSSPS+ S NLLHR KMGD++IIGVID+G WPESE+F+DKGMGP+PSRW+G CE GE FN
Sbjct: 132 SSSPSA-SSNLLHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICESGEDFN 191

Query: 189 WTNCNRKVIGARWYMEGFIADFGKEALATSKEYSSPRDASGHGTHVASTAAGSFVRNVSY 248
            ++CN+KVIGARW+    +AD G+EA+    +Y S RD  GHGTH ASTA G+FVRNVS 
Sbjct: 192 SSHCNKKVIGARWFASALVADHGEEAV--FMDYLSARDNQGHGTHTASTAGGAFVRNVSL 251

Query: 249 RGHGSGTVRGGAPLARLAIYKALWLLNNQASGSSADILMAIDEAISDGVDVLSISIGGSF 308
            G+G GT+RGGAPLARLAIYK LW  ++   GS ADIL  IDEAI DGVDVLS+SIG S 
Sbjct: 252 FGNGRGTLRGGAPLARLAIYKVLW--SDSRLGSGADILKGIDEAIHDGVDVLSMSIGKSI 311

Query: 309 PLFPEFDEVNEIAIGSFHAIARGISVVCSAGNDGPRQQKVVNTAPWILTVAASTMDRAFL 368
           PLF + +E+N +A+GSFHAIA+GISVVC+ GN+G  QQ V N APW+ TVAAST+DRAFL
Sbjct: 312 PLFSDVNELNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRAFL 371

Query: 369 SPITTLGDNTTYFGQSFLNSKKDLVVQLATSLTGRCDEIFGNDTSLSGKVV-ICFSDLAD 428
           + ITTLGDN TY GQ+FL  KKD+V  L  ++ GRC  + G++  +SG VV +CF+DLA 
Sbjct: 372 ASITTLGDNATYLGQTFL--KKDIVGML-LAMDGRCAGLLGSNIHISGNVVLLCFTDLAK 431

Query: 429 IKTTLAAGAAVI-----QANGSGIIVAGQNVDILFPCRQSFPCILVDTDVGTKLFFYTLD 488
                 AGA+ +     QA   GII AGQ+ DIL PC    PCI VDT VGTKLF Y L+
Sbjct: 432 -----KAGASNVVMPGKQAKVVGIIYAGQHNDILGPC--DTPCIHVDTHVGTKLFTYYLN 491

Query: 489 NRNPMIRLGRAKTIIGNPISTKIAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNIF 548
           +    IRL   +TI G PIS++IA+FSSRGPNS SP ILKPDIA PGSNI+AA+ P +  
Sbjct: 492 DEKAFIRLRATRTITGKPISSRIAYFSSRGPNSISPGILKPDIAAPGSNIIAAVPPNHKG 551

Query: 549 SDKGYATQSGTSMAAPHISGIVALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEGS 608
           SDKG+A  SGTSMAAPHISGIVAL+KS+ P WSPAAIKSALITTAR +DPSGLPIFAEGS
Sbjct: 552 SDKGFAAMSGTSMAAPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGS 611

Query: 609 PPKVADPFDYGGGVVDPNAAMDPGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKCP 668
           PPKVADPFDYGGGVVD NAA+DPGLIYDL  TDYI+YYLC MGY  ++IS L+ +KT CP
Sbjct: 612 PPKVADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEAEISHLSQQKTVCP 671

Query: 669 LPRPSILDLNLPTITIPALTNSTTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELAF 728
             R S+LDLNLPTIT+PALTNSTTVTRTVTNVGNLT++Y+A+I+APPG+KV V P+ LAF
Sbjct: 672 SKRASVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLAF 731

Query: 729 NSQVKKISFKVTISTVLHINYGYSFGSLTWTDGVHLVKTPLSVRIHF 770
           NS VKKISFKV  S+ L  NYGYSFGSLTWTDGVHLVK+PLSVR  F
Sbjct: 732 NSNVKKISFKVKFSSSLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDF 756

BLAST of Tan0020887 vs. NCBI nr
Match: KAA0044826.1 (subtilisin-like protease SBT3.7 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 986.9 bits (2550), Expect = 1.0e-283
Identity = 515/768 (67.06%), Postives = 604/768 (78.65%), Query Frame = 0

Query: 6   ASFIVAVMTFYALFSMFAYKPMAEEADHDQDRKVHIVYLGERQHDDIKLTTESHHDLLAT 65
           A  IV  + FY L      +P+  E+ +DQ+ KVHIVYLGE+ H D K TT+SHH LLA 
Sbjct: 8   AHLIVGFIIFYGL-----CEPILAES-NDQNPKVHIVYLGEKPHHDTKFTTDSHHQLLAA 67

Query: 66  VLGSKEKSLESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLRNSIYQMHTTRSWDF 125
           +LGSKEKSLE+MVYSY+HGFSGFAAKLTKS+AQKL+EM  VVRV+ +S+Y++HTTRSWDF
Sbjct: 68  ILGSKEKSLEAMVYSYKHGFSGFAAKLTKSEAQKLSEMSRVVRVVPSSLYKVHTTRSWDF 127

Query: 126 LGLSSSPSSDSDNLLHRGKMGDDIIIGVIDSGIWPESEAFSDKGMGPVPSRWRGRCERGE 185
           LGLSSSP S+S NLLHR K GDD+IIGVIDSGIWPESE+F DKG+GP+PSRW+G CE GE
Sbjct: 128 LGLSSSP-SESSNLLHRAK-GDDVIIGVIDSGIWPESESFKDKGLGPIPSRWKGTCESGE 187

Query: 186 QFNWTNCNRKVIGARWYMEGFIADFGKEALATSKEYSSPRDASGHGTHVASTAAGSFVRN 245
           QFN TNCN+K+IGARW+++ F+AD+G+EALA  KEY SPRD  GHGTH ASTAAGSFV N
Sbjct: 188 QFNSTNCNKKIIGARWFVKAFVADYGREALA--KEYLSPRDLHGHGTHTASTAAGSFVAN 247

Query: 246 VSYRGHGSGTVRGGAPLARLAIYKALWLLNNQASGSSADILMAIDEAISDGVDVLSISIG 305
           ++Y  + +GT RGGAPLARLAIYKALW   N+  GSSADIL AIDEAI DGVDVLSISIG
Sbjct: 248 INYHNNAAGTARGGAPLARLAIYKALW--TNRGVGSSADILKAIDEAIHDGVDVLSISIG 307

Query: 306 GSFPLFPEFDEVNEIAIGSFHAIARGISVVCSAGNDGPRQQKVVNTAPWILTVAASTMDR 365
           GS P +PEF E+++IA GSFHAI +GISVVC+AGN GP  Q V N APWI TVA +T+DR
Sbjct: 308 GSPPFYPEFTELSDIAFGSFHAITKGISVVCAAGNSGPSPQMVDNVAPWIFTVATNTIDR 367

Query: 366 AFLSPITTLGDNTTYFGQSFLNSKKDLVVQLATSLT-GRCDEIFGNDTSLSGKVVICFSD 425
           AFLS ITTL DNTT+                 T+L  GRCD++ GN+  ++GKVV+CF  
Sbjct: 368 AFLSSITTLRDNTTFM----------------TNLEYGRCDQLSGNEAFINGKVVLCFPK 427

Query: 426 LADIKTTLAAGAAVIQANGSGIIVAGQNVD-ILFPCRQS-FPCILVDTDVGTKLFFYTLD 485
           LAD  T   A   V++ANG+GIIVAGQ VD  L  C  S  PCILVDT VG+KLFFY L 
Sbjct: 428 LADYSTISKAAEVVVRANGTGIIVAGQQVDNNLLACISSPIPCILVDTIVGSKLFFYFLQ 487

Query: 486 NR-NPMIRLGRAKTIIGNPISTKIAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNI 545
           N  +P++ L  A+TIIG PI+  IAHFSSRGPNS SP ILKPDI+ PGSNI++A+SP   
Sbjct: 488 NSDHPVVMLRAARTIIGKPIAPTIAHFSSRGPNSVSPPILKPDISAPGSNILSAVSPHYF 547

Query: 546 FSDKGYATQSGTSMAAPHISGIVALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEG 605
           F++KG++  SGTSMA PH+S IVALLKSVHP WSPAAIKSAL+TTAR +   GLPIFA+G
Sbjct: 548 FNEKGFSLMSGTSMATPHVSAIVALLKSVHPTWSPAAIKSALMTTARTEVSPGLPIFADG 607

Query: 606 SPPKVADPFDYGGGVVDPNAAMDPGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKC 665
           +PPKVADPFDYG GVVD NAA+DPGLIYD+   DYI YYLCGMGY+  DIS LTHRKT+C
Sbjct: 608 TPPKVADPFDYGAGVVDANAAVDPGLIYDMGRKDYIDYYLCGMGYEDKDISHLTHRKTEC 667

Query: 666 PLPRPSILDLNLPTITIPALTNSTTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELA 725
           PL R S+LDLNLP ITIP+L NST VTRTVTNVGNL+ +Y+A IEAP G KV+V+PQ L 
Sbjct: 668 PLQRLSLLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAKIEAPFGCKVSVNPQVLV 727

Query: 726 FNSQVKKISFKVTISTVLHINYGYSFGSLTWTDGVHLVKTPLSVRIHF 770
           FNSQVKKISFKV   T +  NYGYSFG LTW+DGVH+VK PLSVR  F
Sbjct: 728 FNSQVKKISFKVMFFTQVQRNYGYSFGRLTWSDGVHVVKIPLSVRFGF 747

BLAST of Tan0020887 vs. ExPASy TrEMBL
Match: A0A0A0KWS6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G047950 PE=3 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 2.3e-294
Identity = 522/764 (68.32%), Postives = 617/764 (80.76%), Query Frame = 0

Query: 9   IVAVMTFYALFSMFAYKPMAEEADHDQDRKVHIVYLGERQHDDIKLTTESHHDLLATVLG 68
           IV  + F  LF     KP+  EAD DQ+ KVHIVYLGE+ H D K T +SHH LL+T+LG
Sbjct: 11  IVGFIIFDCLF-----KPILAEAD-DQNPKVHIVYLGEKPHHDTKFTIDSHHQLLSTILG 70

Query: 69  SKEKSLESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLRNSIYQMHTTRSWDFLGL 128
           SKEKS+E+MVYSY+HGFSGFAAKLTKSQAQKL+EM  VVRV+ +S+Y++HTTRSWDFLGL
Sbjct: 71  SKEKSMEAMVYSYKHGFSGFAAKLTKSQAQKLSEMSRVVRVVPSSLYKVHTTRSWDFLGL 130

Query: 129 SSSPSSDSDNLLHRGKMGDDIIIGVIDSGIWPESEAFSDKGMGPVPSRWRGRCERGEQFN 188
           SSSP  +S NLLHR +MG+++IIGVID+GIWPESE+F DKG+G +PSRW+G CE GEQFN
Sbjct: 131 SSSP-FESSNLLHRAQMGENVIIGVIDTGIWPESESFKDKGVGSIPSRWKGTCESGEQFN 190

Query: 189 WTNCNRKVIGARWYMEGFIADFGKEALATSKEYSSPRDASGHGTHVASTAAGSFVRNVSY 248
            TNCN+K+IGARW+M+GF+AD G++ALA  KEY SPRD +GHGTH AS AAGSFV N++Y
Sbjct: 191 STNCNKKIIGARWFMKGFVADLGRDALA--KEYLSPRDLNGHGTHTASIAAGSFVANINY 250

Query: 249 RGHGSGTVRGGAPLARLAIYKALWLLNNQASGSSADILMAIDEAISDGVDVLSISIGGSF 308
             + +GTVRGGAPLARLAIYKALW     A GS+ADIL AIDEAI+DGVDVLS+SIG   
Sbjct: 251 HNNAAGTVRGGAPLARLAIYKALW--TKDAVGSTADILKAIDEAINDGVDVLSMSIGSLT 310

Query: 309 PLFPEFDEVNEIAIGSFHAIARGISVVCSAGNDGPRQQKVVNTAPWILTVAASTMDRAFL 368
           P  PEF+E N+IA GSFHAIA+GISVVC+AGN GP  Q V N APWI TVAA+T+DRAFL
Sbjct: 311 PFLPEFNEANDIAFGSFHAIAKGISVVCAAGNSGPTPQTVENVAPWIFTVAANTIDRAFL 370

Query: 369 SPITTLGDNTTYFGQSFLNSKKDLVVQLATSLTGRCDEIFGNDTSLSGKVVICFSDLADI 428
           + ITTL DNTT+ GQS L+SKKDLV +L T  TGRCD++ GN+T ++GKVV+CFS+LAD 
Sbjct: 371 ASITTLPDNTTFLGQSLLDSKKDLVAELETLDTGRCDDLLGNETFINGKVVMCFSNLADH 430

Query: 429 KTTLAAGAAVIQANGSGIIVAGQNVDILFPCRQS-FPCILVDTDVGTKLFFYTL--DNRN 488
            T   A  AV +ANG+GIIVAGQ  D LF C  S  PCILVDTDVG+KLFF  L  ++ N
Sbjct: 431 NTIYDAAMAVARANGTGIIVAGQQDDDLFSCIPSPIPCILVDTDVGSKLFFINLLQNSTN 490

Query: 489 PMIRLGRAKTIIGNPISTKIAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNIFSDK 548
           P++RL   +TIIG PI+  I++FSSRGPNS S  ILKPDI+ PGSNI+AA+SP +IF++K
Sbjct: 491 PVVRLRATRTIIGKPITPAISYFSSRGPNSVSNPILKPDISAPGSNILAAVSPHHIFNEK 550

Query: 549 GYATQSGTSMAAPHISGIVALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEGSPPK 608
           G+   SGTSMA PHIS IVALLKSVHP WSPAAIKSAL+TTAR +   GLPIFAEG+PPK
Sbjct: 551 GFMLLSGTSMATPHISAIVALLKSVHPTWSPAAIKSALMTTARTEVSPGLPIFAEGTPPK 610

Query: 609 VADPFDYGGGVVDPNAAMDPGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKCPLPR 668
           +ADPFDYGGG+VD NAA+DPGL+YD+   DYI YYLCGMGYK  DIS LT RKT CPL R
Sbjct: 611 MADPFDYGGGIVDANAAVDPGLVYDMGRKDYIDYYLCGMGYKDEDISHLTQRKTVCPLQR 670

Query: 669 PSILDLNLPTITIPALTNSTTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELAFNSQ 728
            S+LDLNLP ITIP+L NST VTRTVTNVGNL+ +Y+A IE+P G KV+V+PQ L FNSQ
Sbjct: 671 LSVLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAEIESPFGCKVSVNPQVLVFNSQ 730

Query: 729 VKKISFKVTISTVLHINYGYSFGSLTWTDGVHLVKTPLSVRIHF 770
           VKKISFKV   T +  NYGYSFG LTWTDG+H+VK PLSVR  F
Sbjct: 731 VKKISFKVMFFTQVQRNYGYSFGRLTWTDGIHVVKIPLSVRFGF 763

BLAST of Tan0020887 vs. ExPASy TrEMBL
Match: A0A1S3BU23 (subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493167 PE=3 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 1.8e-291
Identity = 523/767 (68.19%), Postives = 613/767 (79.92%), Query Frame = 0

Query: 6   ASFIVAVMTFYALFSMFAYKPMAEEADHDQDRKVHIVYLGERQHDDIKLTTESHHDLLAT 65
           A  IV  + FY L      +P+  E+ +DQ+ KVHIVYLGE+ H D K TT+SHH LLA 
Sbjct: 8   AHLIVGFIIFYGL-----CEPILAES-NDQNPKVHIVYLGEKPHHDTKFTTDSHHQLLAA 67

Query: 66  VLGSKEKSLESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLRNSIYQMHTTRSWDF 125
           +LGSKEKSLE+MVYSY+HGFSGFAAKLTKS+AQKL+EM  VVRV+ +S+Y++HTTRSWDF
Sbjct: 68  ILGSKEKSLEAMVYSYKHGFSGFAAKLTKSEAQKLSEMSRVVRVVPSSLYKVHTTRSWDF 127

Query: 126 LGLSSSPSSDSDNLLHRGKMGDDIIIGVIDSGIWPESEAFSDKGMGPVPSRWRGRCERGE 185
           LGLSSSP S+S NLLHR K GDD+IIGVIDSGIWPESE+F DKG+GP+PSRW+G CE GE
Sbjct: 128 LGLSSSP-SESSNLLHRAK-GDDVIIGVIDSGIWPESESFKDKGLGPIPSRWKGTCESGE 187

Query: 186 QFNWTNCNRKVIGARWYMEGFIADFGKEALATSKEYSSPRDASGHGTHVASTAAGSFVRN 245
           QFN TNCN+K+IGARW+++ F+AD+G+EALA  KEY SPRD  GHGTH ASTAAGSFV N
Sbjct: 188 QFNSTNCNKKIIGARWFVKAFVADYGREALA--KEYLSPRDLHGHGTHTASTAAGSFVAN 247

Query: 246 VSYRGHGSGTVRGGAPLARLAIYKALWLLNNQASGSSADILMAIDEAISDGVDVLSISIG 305
           ++Y  + +GT RGGAPLARLAIYKALW   N+  GSSADIL AIDEAI DGVDVLSISIG
Sbjct: 248 INYHNNAAGTARGGAPLARLAIYKALW--TNRGVGSSADILKAIDEAIHDGVDVLSISIG 307

Query: 306 GSFPLFPEFDEVNEIAIGSFHAIARGISVVCSAGNDGPRQQKVVNTAPWILTVAASTMDR 365
           GS P +PEF E+++IA GSFHAI +GISVVC+AGN GP  Q V N APWI TVA +T+DR
Sbjct: 308 GSPPFYPEFTELSDIAFGSFHAITKGISVVCAAGNSGPSPQMVDNVAPWIFTVATNTIDR 367

Query: 366 AFLSPITTLGDNTTYFGQSFLNSKKDLVVQLATSLTGRCDEIFGNDTSLSGKVVICFSDL 425
           AFLS ITTL DNTT+ GQS L SKKDLV +L +    RCD++ GN+  ++GKVV+CF  L
Sbjct: 368 AFLSSITTLRDNTTFMGQSLLESKKDLVAELVS--WERCDQLSGNEAFINGKVVLCFPKL 427

Query: 426 ADIKTTLAAGAAVIQANGSGIIVAGQNVD-ILFPCRQS-FPCILVDTDVGTKLFFYTLDN 485
           AD  T   A   V++ANG+GIIVAGQ VD  L  C  S  PCILVDT VG+KLFFY L N
Sbjct: 428 ADYSTISKAAEVVVRANGTGIIVAGQQVDNNLLACISSPIPCILVDTIVGSKLFFYFLQN 487

Query: 486 R-NPMIRLGRAKTIIGNPISTKIAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNIF 545
             +P++ L  A+TIIG PI+  IAHFSSRGPNS SP ILKPDI+ PGSNI++A+SP   F
Sbjct: 488 SDHPVVMLRAARTIIGKPIAPTIAHFSSRGPNSVSPPILKPDISAPGSNILSAVSPHYFF 547

Query: 546 SDKGYATQSGTSMAAPHISGIVALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEGS 605
           ++KG++  SGTSMA PH+S IVALLKSVHP WSPAAIKSAL+TTAR +   GLPIFA+G+
Sbjct: 548 NEKGFSLMSGTSMATPHVSAIVALLKSVHPTWSPAAIKSALMTTARTEVSPGLPIFADGT 607

Query: 606 PPKVADPFDYGGGVVDPNAAMDPGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKCP 665
           PPKVADPFDYG GVVD NAA+DPGLIYD+   DYI YYLCGMGY+  DIS LTHRKT+CP
Sbjct: 608 PPKVADPFDYGAGVVDANAAVDPGLIYDMGRKDYIDYYLCGMGYEDKDISHLTHRKTECP 667

Query: 666 LPRPSILDLNLPTITIPALTNSTTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELAF 725
           L R S+LDLNLP ITIP+L NST VTRTVTNVGNL+ +Y+A IEAP G KV+V+PQ L F
Sbjct: 668 LQRLSLLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAKIEAPFGCKVSVNPQVLVF 727

Query: 726 NSQVKKISFKVTISTVLHINYGYSFGSLTWTDGVHLVKTPLSVRIHF 770
           NSQVKKISFKV   T +  NYGYSFG LTW+DGVH+VK PLSVR  F
Sbjct: 728 NSQVKKISFKVMFFTQVQRNYGYSFGRLTWSDGVHVVKIPLSVRFGF 760

BLAST of Tan0020887 vs. ExPASy TrEMBL
Match: A0A6J1JBL4 (subtilisin-like protease SBT3.9 OS=Cucurbita maxima OX=3661 GN=LOC111482985 PE=3 SV=1)

HSP 1 Score: 1001.9 bits (2589), Expect = 1.5e-288
Identity = 525/766 (68.54%), Postives = 611/766 (79.77%), Query Frame = 0

Query: 7   SFIVAVMTFYALFSMFAYKPMAEEADHDQDRKVHIVYLGERQHDDIKLTTESHHDLLATV 66
           SFI+AV+T YA+FS  A      EAD DQ+ KVHIVYLGER +DD+KLTT+SHH+LL +V
Sbjct: 8   SFILAVVTIYAVFSDMA------EAD-DQNSKVHIVYLGERPYDDVKLTTDSHHELLESV 67

Query: 67  LGSKEKSLESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLRNSIYQMHTTRSWDFL 126
           LGSKEKSLESMVYSYRHGFSGFAAKLT SQAQKLA MP V RV  +S+Y+M TTRSWDFL
Sbjct: 68  LGSKEKSLESMVYSYRHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMRTTRSWDFL 127

Query: 127 GLSSSPSSDSDNLLHRGKMGDDIIIGVIDSGIWPESEAFSDKGMGPVPSRWRGRCERGEQ 186
           GLSSSP S+S NL HR KMGD++IIGVID+G WPESE+F+DKGMGP+PSRW+G C+ GE 
Sbjct: 128 GLSSSP-SESSNLFHRAKMGDNVIIGVIDTGFWPESESFNDKGMGPIPSRWKGICQSGED 187

Query: 187 FNWTNCNRKVIGARWYMEGFIADFGKEALATSKEYSSPRDASGHGTHVASTAAGSFVRNV 246
           FN ++CN+KVIGARW+    IAD G+EA+   K+Y S RD  GHGTH ASTA G+FVRNV
Sbjct: 188 FNSSHCNKKVIGARWFASALIADHGEEAV--FKDYLSARDNEGHGTHTASTAGGAFVRNV 247

Query: 247 SYRGHGSGTVRGGAPLARLAIYKALWLLNNQASGSSADILMAIDEAISDGVDVLSISIGG 306
           SY G+G GT+RGGAPLARLAIYK LW  ++   GS ADIL  IDEAI DGVDVLS+SIG 
Sbjct: 248 SYFGNGRGTLRGGAPLARLAIYKVLW--SDSRLGSGADILKGIDEAIHDGVDVLSMSIGK 307

Query: 307 SFPLFPEFDEVNEIAIGSFHAIARGISVVCSAGNDGPRQQKVVNTAPWILTVAASTMDRA 366
           S PLFP+ ++VN +A+GSFHAIA+GISVVC+ GN+G  QQ V N APW+ TVAAST+DRA
Sbjct: 308 SIPLFPDVNDVNPVAVGSFHAIAKGISVVCAGGNEGSIQQTVENVAPWLFTVAASTIDRA 367

Query: 367 FLSPITTLGDNTTYFGQSFLNSKKDLVVQLATSLTGRCDEIFGNDTSLSGKVV-ICFSDL 426
           FL  ITTLGDN TY GQ FL  +KD+V  L  ++ GRC  I G D  +SG VV +CF+DL
Sbjct: 368 FLVSITTLGDNATYLGQMFL--RKDIVGML-VAMDGRCAGILGGDIPISGNVVLLCFTDL 427

Query: 427 ADIKTTLAAGAAV--IQANGSGIIVAGQNVDILFPCRQSFPCILVDTDVGTKLFFYTLDN 486
           A  KT  A+ A +   QA   G+I AGQ  D L PC    PCI VDT VGTKLF Y L++
Sbjct: 428 A--KTAPASNAVMPGKQAKVVGVIYAGQQTDNLVPC--DIPCIHVDTHVGTKLFTYLLND 487

Query: 487 RNPMIRLGRAKTIIGNPISTKIAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNIFS 546
            + +IRL   +TIIG PIS++IA+FSSRGPNS SP ILKPDIA PG+NI+AA+ P +   
Sbjct: 488 DDALIRLRATRTIIGKPISSRIAYFSSRGPNSFSPGILKPDIAAPGANIIAAVPPNHKGG 547

Query: 547 DKGYATQSGTSMAAPHISGIVALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEGSP 606
           DKG+AT SGTSMA PHISGIV L+KS+HP WSPAAIKSALITTAR +DPSG+PIFAEGSP
Sbjct: 548 DKGFATMSGTSMATPHISGIVTLIKSLHPTWSPAAIKSALITTARVEDPSGMPIFAEGSP 607

Query: 607 PKVADPFDYGGGVVDPNAAMDPGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKCPL 666
           PKVADPFDYGGGVVD NAA+DPGLIYDL  TDYI+YYLC MGY  +DIS L+ +KT CP 
Sbjct: 608 PKVADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYLCSMGYTEADISHLSQQKTVCPS 667

Query: 667 PRPSILDLNLPTITIPALTNSTTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELAFN 726
            R SILDLNLPTIT+PAL NSTTVTRTVTNVGNLT++Y+A+I+APPG+KV V P+ L FN
Sbjct: 668 KRASILDLNLPTITVPALINSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVKPRVLVFN 727

Query: 727 SQVKKISFKVTISTVLHINYGYSFGSLTWTDGVHLVKTPLSVRIHF 770
           S VKKISFKV  S+ L  NYGYSFGSLTWTDGVHLVK+PLSVR  F
Sbjct: 728 SNVKKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDF 754

BLAST of Tan0020887 vs. ExPASy TrEMBL
Match: A0A5A7TRR2 (Subtilisin-like protease SBT3.7 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G001180 PE=3 SV=1)

HSP 1 Score: 986.9 bits (2550), Expect = 4.9e-284
Identity = 515/768 (67.06%), Postives = 604/768 (78.65%), Query Frame = 0

Query: 6   ASFIVAVMTFYALFSMFAYKPMAEEADHDQDRKVHIVYLGERQHDDIKLTTESHHDLLAT 65
           A  IV  + FY L      +P+  E+ +DQ+ KVHIVYLGE+ H D K TT+SHH LLA 
Sbjct: 8   AHLIVGFIIFYGL-----CEPILAES-NDQNPKVHIVYLGEKPHHDTKFTTDSHHQLLAA 67

Query: 66  VLGSKEKSLESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLRNSIYQMHTTRSWDF 125
           +LGSKEKSLE+MVYSY+HGFSGFAAKLTKS+AQKL+EM  VVRV+ +S+Y++HTTRSWDF
Sbjct: 68  ILGSKEKSLEAMVYSYKHGFSGFAAKLTKSEAQKLSEMSRVVRVVPSSLYKVHTTRSWDF 127

Query: 126 LGLSSSPSSDSDNLLHRGKMGDDIIIGVIDSGIWPESEAFSDKGMGPVPSRWRGRCERGE 185
           LGLSSSP S+S NLLHR K GDD+IIGVIDSGIWPESE+F DKG+GP+PSRW+G CE GE
Sbjct: 128 LGLSSSP-SESSNLLHRAK-GDDVIIGVIDSGIWPESESFKDKGLGPIPSRWKGTCESGE 187

Query: 186 QFNWTNCNRKVIGARWYMEGFIADFGKEALATSKEYSSPRDASGHGTHVASTAAGSFVRN 245
           QFN TNCN+K+IGARW+++ F+AD+G+EALA  KEY SPRD  GHGTH ASTAAGSFV N
Sbjct: 188 QFNSTNCNKKIIGARWFVKAFVADYGREALA--KEYLSPRDLHGHGTHTASTAAGSFVAN 247

Query: 246 VSYRGHGSGTVRGGAPLARLAIYKALWLLNNQASGSSADILMAIDEAISDGVDVLSISIG 305
           ++Y  + +GT RGGAPLARLAIYKALW   N+  GSSADIL AIDEAI DGVDVLSISIG
Sbjct: 248 INYHNNAAGTARGGAPLARLAIYKALW--TNRGVGSSADILKAIDEAIHDGVDVLSISIG 307

Query: 306 GSFPLFPEFDEVNEIAIGSFHAIARGISVVCSAGNDGPRQQKVVNTAPWILTVAASTMDR 365
           GS P +PEF E+++IA GSFHAI +GISVVC+AGN GP  Q V N APWI TVA +T+DR
Sbjct: 308 GSPPFYPEFTELSDIAFGSFHAITKGISVVCAAGNSGPSPQMVDNVAPWIFTVATNTIDR 367

Query: 366 AFLSPITTLGDNTTYFGQSFLNSKKDLVVQLATSLT-GRCDEIFGNDTSLSGKVVICFSD 425
           AFLS ITTL DNTT+                 T+L  GRCD++ GN+  ++GKVV+CF  
Sbjct: 368 AFLSSITTLRDNTTFM----------------TNLEYGRCDQLSGNEAFINGKVVLCFPK 427

Query: 426 LADIKTTLAAGAAVIQANGSGIIVAGQNVD-ILFPCRQS-FPCILVDTDVGTKLFFYTLD 485
           LAD  T   A   V++ANG+GIIVAGQ VD  L  C  S  PCILVDT VG+KLFFY L 
Sbjct: 428 LADYSTISKAAEVVVRANGTGIIVAGQQVDNNLLACISSPIPCILVDTIVGSKLFFYFLQ 487

Query: 486 NR-NPMIRLGRAKTIIGNPISTKIAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNI 545
           N  +P++ L  A+TIIG PI+  IAHFSSRGPNS SP ILKPDI+ PGSNI++A+SP   
Sbjct: 488 NSDHPVVMLRAARTIIGKPIAPTIAHFSSRGPNSVSPPILKPDISAPGSNILSAVSPHYF 547

Query: 546 FSDKGYATQSGTSMAAPHISGIVALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEG 605
           F++KG++  SGTSMA PH+S IVALLKSVHP WSPAAIKSAL+TTAR +   GLPIFA+G
Sbjct: 548 FNEKGFSLMSGTSMATPHVSAIVALLKSVHPTWSPAAIKSALMTTARTEVSPGLPIFADG 607

Query: 606 SPPKVADPFDYGGGVVDPNAAMDPGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKC 665
           +PPKVADPFDYG GVVD NAA+DPGLIYD+   DYI YYLCGMGY+  DIS LTHRKT+C
Sbjct: 608 TPPKVADPFDYGAGVVDANAAVDPGLIYDMGRKDYIDYYLCGMGYEDKDISHLTHRKTEC 667

Query: 666 PLPRPSILDLNLPTITIPALTNSTTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELA 725
           PL R S+LDLNLP ITIP+L NST VTRTVTNVGNL+ +Y+A IEAP G KV+V+PQ L 
Sbjct: 668 PLQRLSLLDLNLPAITIPSLVNSTIVTRTVTNVGNLSCVYKAKIEAPFGCKVSVNPQVLV 727

Query: 726 FNSQVKKISFKVTISTVLHINYGYSFGSLTWTDGVHLVKTPLSVRIHF 770
           FNSQVKKISFKV   T +  NYGYSFG LTW+DGVH+VK PLSVR  F
Sbjct: 728 FNSQVKKISFKVMFFTQVQRNYGYSFGRLTWSDGVHVVKIPLSVRFGF 747

BLAST of Tan0020887 vs. ExPASy TrEMBL
Match: A0A6J1ETU3 (subtilisin-like protease SBT3.7 isoform X5 OS=Cucurbita moschata OX=3662 GN=LOC111437654 PE=3 SV=1)

HSP 1 Score: 984.6 bits (2544), Expect = 2.4e-283
Identity = 516/767 (67.28%), Postives = 603/767 (78.62%), Query Frame = 0

Query: 4   KQASFIVAVMTFYALFSMFAYKPMAEEADHDQDRKVHIVYLGERQHDDIKLTTESHHDLL 63
           + +SF+ AV+T YA+FS  A      EAD +Q+ KVHIVYLGER +DD+KLTT SHH+LL
Sbjct: 5   RASSFMHAVVTIYAVFSAMA------EAD-NQNSKVHIVYLGERPYDDVKLTTHSHHELL 64

Query: 64  ATVLGSKEKSLESMVYSYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLRNSIYQMHTTRSW 123
            +VL SKEKSLESMVYSY+HGFSGFAAKLT SQAQKLA MP V RV  +S+Y+MHTTRSW
Sbjct: 65  ESVLRSKEKSLESMVYSYKHGFSGFAAKLTNSQAQKLARMPIVARVFPSSLYKMHTTRSW 124

Query: 124 DFLGLSSSPSSDSDNLLHRGKMGDDIIIGVIDSGIWPESEAFSDKGMGPVPSRWRGRCER 183
           DFLGLSSSPS+ S NLLHR KMGD++IIGVIDSG WPESE+F+DKGMGP+PSRW+G C+ 
Sbjct: 125 DFLGLSSSPSA-SSNLLHRAKMGDNVIIGVIDSGFWPESESFNDKGMGPIPSRWKGICQL 184

Query: 184 GEQFNWTNCNRKVIGARWYMEGFIADFGKEALATSKEYSSPRDASGHGTHVASTAAGSFV 243
           GE FN  +CN+KVIGARW+    IAD G+EA+   K+Y S RD  GHGTH ASTA G+FV
Sbjct: 185 GEDFNSFHCNKKVIGARWFARALIADHGEEAV--FKDYLSARDNKGHGTHTASTAGGAFV 244

Query: 244 RNVSYRGHGSGTVRGGAPLARLAIYKALWLLNNQASGSSADILMAIDEAISDGVDVLSIS 303
           RNVSY G+  GT+RGGAPLARLAIYK +W  +++  GS+ADIL  IDEAI DGVDVLS+S
Sbjct: 245 RNVSYFGNRRGTLRGGAPLARLAIYKVIW--SDRRLGSAADILKGIDEAIHDGVDVLSMS 304

Query: 304 IGGSFPLFPEFDEVNEIAIGSFHAIARGISVVCSAGNDGPRQQKVVNTAPWILTVAASTM 363
           IGGS PL P+  E N +AIGSFHAIARG+SVVC+ GN+G  QQ VVN APW+ TVAAST+
Sbjct: 305 IGGSIPLPPDVSEWNTVAIGSFHAIARGVSVVCAGGNEGSIQQTVVNVAPWLFTVAASTI 364

Query: 364 DRAFLSPITTLGDNTTYFGQSFLNSKKDLVVQLATSLTGRCDEIFGNDTSLSGKVV-ICF 423
           DRAFL+ ITTLGDN TY GQ+FL  KKD+V +L   +  RC    G+D    G VV +CF
Sbjct: 365 DRAFLTSITTLGDNATYLGQTFL--KKDVVGKLVV-MDRRCAGHLGSDIPTGGNVVLLCF 424

Query: 424 SDLADIKTTLAAGAAVIQANGSGIIVAGQNVDILFPCRQSFPCILVDTDVGTKLFFYTLD 483
            DLA           V QA   G+I AGQ+ DIL PC    PCI VDT VGTKL  Y L 
Sbjct: 425 IDLAMKAAASNPVMPVKQAKVVGVIYAGQHTDILGPC--DVPCIHVDTHVGTKLLTYILS 484

Query: 484 NRNPMIRLGRAKTIIGNPISTKIAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNIF 543
           +   +IRL   +TI+G PIS++IA+FSSRGPNS SP ILKPDIA PGSNI+AA+ P +  
Sbjct: 485 DEKALIRLKATRTIVGKPISSRIAYFSSRGPNSVSPGILKPDIAAPGSNIIAAVPPNHKG 544

Query: 544 SDKGYATQSGTSMAAPHISGIVALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEGS 603
            DKG+A  SGTSMA PHISGIVAL+KS+ P WSPAAIKSALITTAR +DPSGLPIFAEGS
Sbjct: 545 GDKGFAAMSGTSMATPHISGIVALIKSLRPTWSPAAIKSALITTARVEDPSGLPIFAEGS 604

Query: 604 PPKVADPFDYGGGVVDPNAAMDPGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKCP 663
           PPKVADPFDYGGGVVD NAA+DPGLIYDL  TDYI+YY+C MGY  ++IS L+ +K  CP
Sbjct: 605 PPKVADPFDYGGGVVDANAAVDPGLIYDLGATDYIYYYMCSMGYTKAEISHLSQQKIVCP 664

Query: 664 LPRPSILDLNLPTITIPALTNSTTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELAF 723
             R S+LDLNLPTIT+PALTNSTTVTRTVTNVGNLT++Y+A+I+APPG+KV V+P+ L F
Sbjct: 665 SKRASVLDLNLPTITVPALTNSTTVTRTVTNVGNLTAVYKAVIKAPPGSKVRVNPRVLVF 724

Query: 724 NSQVKKISFKVTISTVLHINYGYSFGSLTWTDGVHLVKTPLSVRIHF 770
           NS VKKISFKV  S+ L  NYGYSFGSLTWTDGVHLVK+PLSVR  F
Sbjct: 725 NSNVKKISFKVKFSSTLQRNYGYSFGSLTWTDGVHLVKSPLSVRFDF 754

BLAST of Tan0020887 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 781.6 bits (2017), Expect = 6.0e-226
Identity = 400/763 (52.42%), Postives = 535/763 (70.12%), Query Frame = 0

Query: 20  SMFAYKPMAEEADHDQDRKVHIVYLGERQHDDIKLTTESHHDLLATVLGSKEKSLESMVY 79
           ++F  K  +  A+    RKVHIVYLGE+QHDD +  TESHH +L ++LGSKE + +SMVY
Sbjct: 31  TIFLTKERSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVY 90

Query: 80  SYRHGFSGFAAKLTKSQAQKLAEMPGVVRVLRNSIYQMHTTRSWDFLGLSSSPSSDSDNL 139
           SYRHGFSGFAAKLT+SQA+K+A++P VV V+ +S Y++ TTR+WD+LGLS   +++  +L
Sbjct: 91  SYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLS---AANPKSL 150

Query: 140 LHRGKMGDDIIIGVIDSGIWPESEAFSDKGMGPVPSRWRGRCERGEQFNWTNCNRKVIGA 199
           LH   MG+ IIIGVID+G+WPESE F+D G GPVPS W+G CE GE FN +NCN+K+IGA
Sbjct: 151 LHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKLIGA 210

Query: 200 RWYMEGFIADFGKEALATSKEYSSPRDASGHGTHVASTAAGSFVRNVSYRGHGSGTVRGG 259
           ++++ GF+A+        S ++ SPRD  GHGTHV++ A GSFV N+SY+G   GTVRGG
Sbjct: 211 KYFINGFLAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGG 270

Query: 260 APLARLAIYKALWLLNNQ--ASGSSADILMAIDEAISDGVDVLSISIGGSFPLFPEFDEV 319
           AP A +A+YKA W L++    + SSADIL A+DEA+ DGVDVLSIS+G S PL+ E D  
Sbjct: 271 APRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGETDIR 330

Query: 320 NEIAIGSFHAIARGISVVCSAGNDGPRQQKVVNTAPWILTVAASTMDRAFLSPITTLGDN 379
           + I  G+FHA+ +GI+VVCS GN GP    V NTAPWI+TVAA+T+DR+F +P+ TLG+N
Sbjct: 331 DGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPL-TLGNN 390

Query: 380 TTYFGQSFLNSKKDLVVQLA---------TSLTGRCDE-IFGNDTSLSGKVVICFSDLAD 439
               GQ+           L           S +G C+E +F ++ ++ GKVV+CF+    
Sbjct: 391 KVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKVVLCFTTSPY 450

Query: 440 IKTTLAAGAAVIQANGSGIIVAGQNVDILFPCRQSFPCILVDTDVGTKLFFYTLDNRNPM 499
               L+A   V +A G G+I+A      + PC   FPC+ VD ++GT +  YT  + +P+
Sbjct: 451 GGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPV 510

Query: 500 IRLGRAKTIIGNPISTKIAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNIFSDKGY 559
           +++  +KT++G P+ TK+A FSSRGPNS +PAILKPDIA PG +I+AA +    FSD+G+
Sbjct: 511 VKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAA-TTNTTFSDQGF 570

Query: 560 ATQSGTSMAAPHISGIVALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEGSPPKVA 619
              SGTSMAAP ISG+ ALLK++H +WSPAAI+SA++TTA   DP G  IFAEGSPPK+A
Sbjct: 571 IMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFAEGSPPKLA 630

Query: 620 DPFDYGGGVVDPNAAMDPGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKCPLPRPS 679
           DPFDYGGG+V+P  + +PGL+YD+   DY+  Y+C +GY  + IS L  + T C  P+PS
Sbjct: 631 DPFDYGGGLVNPEKSANPGLVYDMGLEDYV-LYMCSVGYNETSISQLIGKTTVCSNPKPS 690

Query: 680 ILDLNLPTITIPALTNSTTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELAFNSQVK 739
           +LD NLP+ITIP L +  T+TRTVTNVG L S+YR  +E P G +VTV P+ L FNS  K
Sbjct: 691 VLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNSTTK 750

Query: 740 KISFKVTISTVLHINYGYSFGSLTWTDGVHLVKTPLSVRIHFL 771
           K+ FKV +ST    N GY FGSLTW+D +H V  PLSVR   L
Sbjct: 751 KVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQIL 787

BLAST of Tan0020887 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 780.4 bits (2014), Expect = 1.3e-225
Identity = 398/752 (52.93%), Postives = 530/752 (70.48%), Query Frame = 0

Query: 31  ADHDQDRKVHIVYLGERQHDDIKLTTESHHDLLATVLGSKEKSLESMVYSYRHGFSGFAA 90
           A+    RKVHIVYLGE+QHDD +  TESHH +L ++LGSKE + +SMVYSYRHGFSGFAA
Sbjct: 26  AESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDANDSMVYSYRHGFSGFAA 85

Query: 91  KLTKSQAQKLAEMPGVVRVLRNSIYQMHTTRSWDFLGLSSSPSSDSDNLLHRGKMGDDII 150
           KLT+SQA+K+A++P VV V+ +S Y++ TTR+WD+LGLS   +++  +LLH   MG+ II
Sbjct: 86  KLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGLS---AANPKSLLHETNMGEQII 145

Query: 151 IGVIDSGIWPESEAFSDKGMGPVPSRWRGRCERGEQFNWTNCNRKVIGARWYMEGFIADF 210
           IGVID+G+WPESE F+D G GPVPS W+G CE GE FN +NCN+K+IGA++++ GF+A+ 
Sbjct: 146 IGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSNCNKKLIGAKYFINGFLAEN 205

Query: 211 GKEALATSKEYSSPRDASGHGTHVASTAAGSFVRNVSYRGHGSGTVRGGAPLARLAIYKA 270
                  S ++ SPRD  GHGTHV++ A GSFV N+SY+G   GTVRGGAP A +A+YKA
Sbjct: 206 ESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYKGLAGGTVRGGAPRAHIAMYKA 265

Query: 271 LWLLNNQ--ASGSSADILMAIDEAISDGVDVLSISIGGSFPLFPEFDEVNEIAIGSFHAI 330
            W L++    + SSADIL A+DEA+ DGVDVLSIS+G S PL+ E D  + I  G+FHA+
Sbjct: 266 CWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSSVPLYGETDIRDGITTGAFHAV 325

Query: 331 ARGISVVCSAGNDGPRQQKVVNTAPWILTVAASTMDRAFLSPITTLGDNTTYFGQSFLNS 390
            +GI+VVCS GN GP    V NTAPWI+TVAA+T+DR+F +P+ TLG+N    GQ+    
Sbjct: 326 LKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSFATPL-TLGNNKVILGQAMYTG 385

Query: 391 KKDLVVQLA---------TSLTGRCDE-IFGNDTSLSGKVVICFSDLADIKTTLAAGAAV 450
                  L           S +G C+E +F ++ ++ GKVV+CF+        L+A   V
Sbjct: 386 PGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKVVLCFTTSPYGGAVLSAARYV 445

Query: 451 IQANGSGIIVAGQNVDILFPCRQSFPCILVDTDVGTKLFFYTLDNRNPMIRLGRAKTIIG 510
            +A G G+I+A      + PC   FPC+ VD ++GT +  YT  + +P++++  +KT++G
Sbjct: 446 KRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILLYTRSSGSPVVKIQPSKTLVG 505

Query: 511 NPISTKIAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNIFSDKGYATQSGTSMAAP 570
            P+ TK+A FSSRGPNS +PAILKPDIA PG +I+AA +    FSD+G+   SGTSMAAP
Sbjct: 506 QPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAA-TTNTTFSDQGFIMLSGTSMAAP 565

Query: 571 HISGIVALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEGSPPKVADPFDYGGGVVD 630
            ISG+ ALLK++H +WSPAAI+SA++TTA   DP G  IFAEGSPPK+ADPFDYGGG+V+
Sbjct: 566 AISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIFAEGSPPKLADPFDYGGGLVN 625

Query: 631 PNAAMDPGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKCPLPRPSILDLNLPTITI 690
           P  + +PGL+YD+   DY+  Y+C +GY  + IS L  + T C  P+PS+LD NLP+ITI
Sbjct: 626 PEKSANPGLVYDMGLEDYV-LYMCSVGYNETSISQLIGKTTVCSNPKPSVLDFNLPSITI 685

Query: 691 PALTNSTTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELAFNSQVKKISFKVTISTV 750
           P L +  T+TRTVTNVG L S+YR  +E P G +VTV P+ L FNS  KK+ FKV +ST 
Sbjct: 686 PNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPETLVFNSTTKKVYFKVKVSTT 745

Query: 751 LHINYGYSFGSLTWTDGVHLVKTPLSVRIHFL 771
              N GY FGSLTW+D +H V  PLSVR   L
Sbjct: 746 HKTNTGYYFGSLTWSDSLHNVTIPLSVRTQIL 771

BLAST of Tan0020887 vs. TAIR 10
Match: AT4G10540.1 (Subtilase family protein )

HSP 1 Score: 777.7 bits (2007), Expect = 8.6e-225
Identity = 402/747 (53.82%), Postives = 526/747 (70.41%), Query Frame = 0

Query: 36  DRKVHIVYLGERQHDDIKLTTESHHDLLATVLGSKEKSLESMVYSYRHGFSGFAAKLTKS 95
           + KVHIVYLGE+QHDD +  TESHH +L ++LGSKE +  SMV+SYRHGFSGFAAKLTKS
Sbjct: 27  ESKVHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEDAHSSMVHSYRHGFSGFAAKLTKS 86

Query: 96  QAQKLAEMPGVVRVLRNSIYQMHTTRSWDFLGLSSSPSSDSDNLLHRGKMGDDIIIGVID 155
           QA+KLA++P VV V  +S YQ+ TTR+WD+LGLS    ++  NLL+   MG+++IIG++D
Sbjct: 87  QAKKLADLPEVVHVTPDSFYQLDTTRTWDYLGLS---VANPKNLLNDTNMGEEVIIGIVD 146

Query: 156 SGIWPESEAFSDKGMGPVPSRWRGRCERGEQFNWTNCNRKVIGARWYMEGFIADFGKEAL 215
           SG+WPESE F+D G+GPVPS W+G C  GE F  + CN+K+IGA++++ GF+A       
Sbjct: 147 SGVWPESEVFNDNGIGPVPSHWKGGCVSGENFTSSQCNKKLIGAKYFINGFLATHESFNS 206

Query: 216 ATSKEYSSPRDASGHGTHVASTAAGSFVRNVSYRGHGSGTVRGGAPLARLAIYKALWLLN 275
             S ++ SPRD SGHGTHVA+ A GS+V ++SY+G   GTVRGGAP AR+A+YKA W L+
Sbjct: 207 TESLDFISPRDRSGHGTHVATIAGGSYVPSISYKGLAGGTVRGGAPRARIAMYKACWYLD 266

Query: 276 --NQASGSSADILMAIDEAISDGVDVLSISIGGSFPLFPEFDEVNEIAIGSFHAIARGIS 335
             +  + SSADIL A+DEA+ DGVDVLS+SIG  FP FPE D    IA G+FHA+ +GI+
Sbjct: 267 RFDINTCSSADILKAMDEAMHDGVDVLSLSIGYRFPYFPETDVRAVIATGAFHAVLKGIT 326

Query: 336 VVCSAGNDGPRQQKVVNTAPWILTVAASTMDRAFLSPITTLGDNTTYFGQSFLNSKKDLV 395
           VVCS GN GP  Q V NTAPWILTVAA+T+DR+F +PI TLG+N    GQ+     +   
Sbjct: 327 VVCSGGNSGPAAQTVGNTAPWILTVAATTLDRSFPTPI-TLGNNKLILGQAMYTGPELGF 386

Query: 396 VQLA---------TSLTGRCDEIFGNDT-SLSGKVVICFSDLADIKTTLAAGAAVIQANG 455
             L           S +G C+ +F N   +++GKVV+CF+      T  +A + V +A G
Sbjct: 387 TSLVYPENPGNSNESFSGDCELLFFNSNHTMAGKVVLCFTTSTRYITVSSAVSYVKEAGG 446

Query: 456 SGIIVAGQNVDILFPCRQSFPCILVDTDVGTKLFFYTLDNRNPMIRLGRAKTIIGNPIST 515
            G+IVA    D L PC   FPC+ VD ++GT +  Y      P++++  +KT++G P+ T
Sbjct: 447 LGVIVARNPGDNLSPCEDDFPCVAVDYELGTDILLYIRSTGLPVVKIQPSKTLVGQPVGT 506

Query: 516 KIAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNIFSDKGYATQSGTSMAAPHISGI 575
           K+A FSSRGPNS  PAILKPDIA PG +I+AA +    F+D+G+   SGTSMAAP ISG+
Sbjct: 507 KVADFSSRGPNSIEPAILKPDIAAPGVSILAATTTNKTFNDRGFIFLSGTSMAAPTISGV 566

Query: 576 VALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEGSPPKVADPFDYGGGVVDPNAAM 635
           VALLK++H +WSPAAI+SA++TTA   DP G  IFAEGSP K+ADPFDYGGG+V+P  A 
Sbjct: 567 VALLKALHRDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKLADPFDYGGGLVNPEKAA 626

Query: 636 DPGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKCPLPRPSILDLNLPTITIPALTN 695
            PGL+YDL   DY+  Y+C +GY  + IS L  + T C  P+PS+LD NLP+ITIP L +
Sbjct: 627 KPGLVYDLGLEDYV-LYMCSVGYNETSISQLVGKGTVCSNPKPSVLDFNLPSITIPNLKD 686

Query: 696 STTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELAFNSQVKKISFKVTISTVLHINY 755
             T+TRT+TNVG L S+Y+ +IE P G +VTV P+ L FNS  K++SFKV +ST   IN 
Sbjct: 687 EVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVTPETLLFNSTTKRVSFKVKVSTTHKINT 746

Query: 756 GYSFGSLTWTDGVHLVKTPLSVRIHFL 771
           GY FGSLTW+D +H V  PLSVR   L
Sbjct: 747 GYFFGSLTWSDSLHNVTIPLSVRTQIL 768

BLAST of Tan0020887 vs. TAIR 10
Match: AT1G32950.1 (Subtilase family protein )

HSP 1 Score: 768.8 bits (1984), Expect = 4.0e-222
Identity = 401/746 (53.75%), Postives = 523/746 (70.11%), Query Frame = 0

Query: 38  KVHIVYLGERQHDDIKLTTESHHDLLATVLGSKEKSLESMVYSYRHGFSGFAAKLTKSQA 97
           KVHIVYLGE+QHDD K  TESHH +L+++LGSK+ + ESMVYSYRHGFSGFAAKLTKSQA
Sbjct: 28  KVHIVYLGEKQHDDPKFVTESHHQMLSSLLGSKDDAHESMVYSYRHGFSGFAAKLTKSQA 87

Query: 98  QKLAEMPGVVRVLRNSIYQMHTTRSWDFLGLSSSPSSD-SDNLLHRGKMGDDIIIGVIDS 157
           +K+A+ P V+ V+ +S Y++ TTR WD+LG    PS+D S NL+    MGD  IIGVID+
Sbjct: 88  KKIADSPEVIHVIPDSYYELATTRIWDYLG----PSADNSKNLVSDTNMGDQTIIGVIDT 147

Query: 158 GIWPESEAFSDKGMGPVPSRWRGRCERGEQFNWTNCNRKVIGARWYMEGFIADFGKEALA 217
           G+WPESE+F+D G+GPVPS W+G CE GE F  TNCNRK+IGA++++ GF+A+    A  
Sbjct: 148 GVWPESESFNDYGVGPVPSHWKGGCEPGENFISTNCNRKLIGAKYFINGFLAENQFNA-T 207

Query: 218 TSKEYSSPRDASGHGTHVASTAAGSFVRNVSYRGHGSGTVRGGAPLARLAIYKALWLLN- 277
            S +Y S RD  GHGTHVAS A GSFV NVSY+G G GT+RGGAP AR+A+YKA W +N 
Sbjct: 208 ESPDYISARDFDGHGTHVASIAGGSFVPNVSYKGLGRGTLRGGAPRARIAMYKACWYINE 267

Query: 278 -NQASGSSADILMAIDEAISDGVDVLSISIGGSFPLFPEFDEVNEIAIGSFHAIARGISV 337
            +  + S +DI+ AIDEAI DGVDVLSIS+GG  PL  E D  + IA G+FHA+A+GI V
Sbjct: 268 LDGVTCSFSDIMKAIDEAIHDGVDVLSISLGGRVPLNSETDLRDGIATGAFHAVAKGIVV 327

Query: 338 VCSAGNDGPRQQKVVNTAPWILTVAASTMDRAFLSPITTLGDNTTYFGQSFLNSKKDLVV 397
           VC+ GN GP  Q VVNTAPWILTVAA+T+DR+F +PI  LG+N    GQ+     +    
Sbjct: 328 VCAGGNAGPSSQTVVNTAPWILTVAATTLDRSFATPI-ILGNNQVILGQAMYIGPELGFT 387

Query: 398 QLA---------TSLTGRCDEI-FGNDTSLSGKVVICFSDLADIKTTLAAGAAVIQANGS 457
            L           + +G C+ +   ++ +++GKVV+CF+   D      A + V  A G 
Sbjct: 388 SLVYPEDPGNSIDTFSGVCESLNLNSNRTMAGKVVLCFTTARDFTVVSTAASIVKAAGGL 447

Query: 458 GIIVAGQNVDILFPCRQSFPCILVDTDVGTKLFFYTLDNRNPMIRLGRAKTIIGNPISTK 517
           G+I+A      L PC   FPC+ +D ++GT + FY     +P++++  ++T++G P+ TK
Sbjct: 448 GLIIARNPGYNLAPCSDDFPCVAIDNELGTDILFYIRYTGSPVVKIQPSRTLVGEPVGTK 507

Query: 518 IAHFSSRGPNSASPAILKPDIAGPGSNIVAAISPRNIFSDKGYATQSGTSMAAPHISGIV 577
           +A FSSRGPNS SPAILKPDIA PG +I+AA SP +  +  G+  +SGTSMAAP ISG++
Sbjct: 508 VATFSSRGPNSISPAILKPDIAAPGVSILAATSPNDTLNAGGFVMRSGTSMAAPVISGVI 567

Query: 578 ALLKSVHPNWSPAAIKSALITTARAKDPSGLPIFAEGSPPKVADPFDYGGGVVDPNAAMD 637
           ALLKS+HP+WSPAA +SA++TTA   DP G  I AE S  KV DPFDYGGG+V+P  A +
Sbjct: 568 ALLKSLHPDWSPAAFRSAIVTTAWRTDPFGEQIAAESSSLKVPDPFDYGGGLVNPEKAAE 627

Query: 638 PGLIYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKCPLPRPSILDLNLPTITIPALTNS 697
           PGLI D+D+ DY+  YLC  GY  S IS L  + T C  P+PS+LD+NLP+ITIP L + 
Sbjct: 628 PGLILDMDSQDYV-LYLCSAGYNDSSISRLVGKVTVCSNPKPSVLDINLPSITIPNLKDE 687

Query: 698 TTVTRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELAFNSQVKKISFKVTISTVLHINYG 757
            T+TRTVTNVG + S+Y+ L+E P G +V V P+ L FNS+ K +SF V +ST   IN G
Sbjct: 688 VTLTRTVTNVGPVDSVYKVLVEPPLGIQVVVTPETLVFNSKTKSVSFTVIVSTTHKINTG 747

Query: 758 YSFGSLTWTDGVHLVKTPLSVRIHFL 771
           + FGSLTWTD +H V  P+SVR   L
Sbjct: 748 FYFGSLTWTDSIHNVVIPVSVRTQIL 766

BLAST of Tan0020887 vs. TAIR 10
Match: AT4G10510.1 (Subtilase family protein )

HSP 1 Score: 766.9 bits (1979), Expect = 1.5e-221
Identity = 394/743 (53.03%), Postives = 529/743 (71.20%), Query Frame = 0

Query: 39  VHIVYLGERQHDDIKLTTESHHDLLATVLGSKEKSLESMVYSYRHGFSGFAAKLTKSQAQ 98
           VHIVYLGE+QHDD +  TESHH +L ++LGSKE++  SMV+S+RHGFSGFAAKLT+SQA+
Sbjct: 22  VHIVYLGEKQHDDPEFVTESHHRMLWSLLGSKEEAHGSMVHSFRHGFSGFAAKLTESQAK 81

Query: 99  KLAEMPGVVRVLRNSIYQMHTTRSWDFLGLSSSPSSDSDNLLHRGKMGDDIIIGVIDSGI 158
           K+A++P VV V+ +  Y+  TTR+WD+LGLS    ++  NLL++  MG+ +IIG+IDSG+
Sbjct: 82  KIADLPEVVHVIPDRFYKPATTRTWDYLGLS---PTNPKNLLNQTNMGEQMIIGIIDSGV 141

Query: 159 WPESEAFSDKGMGPVPSRWRGRCERGEQFNWTNCNRKVIGARWYMEGFIADFGKEALATS 218
           WPESE F+D  +GPVPS W+G CE GE FN ++CN+K+IGA++++  F+A       + S
Sbjct: 142 WPESEVFNDNEIGPVPSHWKGGCESGEDFNSSHCNKKLIGAKYFINAFLATHESFNSSES 201

Query: 219 KEYSSPRDASGHGTHVASTAAGSFVRNVSYRGHGSGTVRGGAPLARLAIYKALWLLN-NQ 278
            ++ SPR  +GHGTHVA+ A GS+V N SY+G   GTVRGGAP AR+A+YK  W L+ + 
Sbjct: 202 LDFISPRGYNGHGTHVATIAGGSYVPNTSYKGLAGGTVRGGAPRARIAVYKTCWYLDLDI 261

Query: 279 ASGSSADILMAIDEAISDGVDVLSISIGGSFPLFPEFDEVNEIAIGSFHAIARGISVVCS 338
           A+ SSADIL A+DEAI DGVDVLS+S+G   PL+PE D  + IA G+FHA+ +GI+VVC+
Sbjct: 262 AACSSADILKAMDEAIHDGVDVLSLSLGFE-PLYPETDVRDGIATGAFHAVLKGITVVCA 321

Query: 339 AGNDGPRQQKVVNTAPWILTVAASTMDRAFLSPITTLGDNTTYFGQSFLNSKKDLVVQLA 398
           AGN GP  Q V NTAPWILTVAA+T+DR+F++P+ TLG+N    GQ+     +     L 
Sbjct: 322 AGNAGPAAQTVGNTAPWILTVAATTLDRSFVTPM-TLGNNKVILGQAIYTGTEVGFTSLV 381

Query: 399 ---------TSLTGRCDEIFGN-DTSLSGKVVICFSDLADIKTTLAAGAAVIQANGSGII 458
                     S +G C+ +  N + +++GKVV+CF++     +   A   V +A G G+I
Sbjct: 382 YPENPGNSNESFSGTCERLLINSNRTMAGKVVLCFTESPYSISVTRAAHYVKRAGGLGVI 441

Query: 459 VAGQNVDILFPCRQSFPCILVDTDVGTKLFFYTLDNRNPMIRLGRAKTIIGNPISTKIAH 518
           +AGQ  ++L PC   FPC+ VD ++GT + FY   N +P++++  ++T+IG P+ TK+A 
Sbjct: 442 IAGQPGNVLRPCLDDFPCVAVDYELGTYILFYIRSNGSPVVKIQPSRTLIGQPVGTKVAS 501

Query: 519 FSSRGPNSASPAILKPDIAGPGSNIVAAISPRNIFSDKGYATQSGTSMAAPHISGIVALL 578
           FSSRGPN  S AILKPDIA PG +I+AA +    F+D+G+   SGTSMA P ISGIVALL
Sbjct: 502 FSSRGPNPISAAILKPDIAAPGVSILAATTTNTTFNDRGFIFLSGTSMATPTISGIVALL 561

Query: 579 KSVHPNWSPAAIKSALITTARAKDPSGLPIFAEGSPPKVADPFDYGGGVVDPNAAMDPGL 638
           K++HP+WSPAAI+SA++TTA   DP G  IFAEGSP K ADPFDYGGG+V+P  A  PGL
Sbjct: 562 KALHPDWSPAAIRSAIVTTAWRTDPFGEQIFAEGSPRKPADPFDYGGGLVNPEKATKPGL 621

Query: 639 IYDLDTTDYIHYYLCGMGYKLSDISLLTHRKTKCPLPRPSILDLNLPTITIPALTNSTTV 698
           +YDL   DY+  Y+C +GY  + IS L  + T C  P+PS+LD NLP+ITIP L    T+
Sbjct: 622 VYDLGLEDYV-LYMCSVGYNETSISQLVGKGTVCSYPKPSVLDFNLPSITIPNLKEEVTL 681

Query: 699 TRTVTNVGNLTSIYRALIEAPPGTKVTVDPQELAFNSQVKKISFKVTISTVLHINYGYSF 758
            RT+TNVG L S+YR  +E P GT+VTV P+ L FNS  K++SFKV++ST   IN GY F
Sbjct: 682 PRTLTNVGPLESVYRVAVEPPLGTQVTVTPETLVFNSTTKRVSFKVSVSTTHKINTGYYF 741

Query: 759 GSLTWTDGVHLVKTPLSVRIHFL 771
           GSLTW+D +H V  PLSVR   L
Sbjct: 742 GSLTWSDSLHNVTIPLSVRTQLL 758