Tan0020673 (gene) Snake gourd v1

Overview


Name	Tan0020673
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Alpha-galactosidase
Location	LG09: 57331872 .. 57334009 (-)
RNA-Seq Expression	Tan0020673
Synteny	Tan0020673

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGGTTTCGGTAACGGACCTAGACTTCAAGTTGCGTCTACATCCGATCCGATGATCTCACGCGTTTTCGAGCCAATTCCACGGGTACGGTTTTTGCTTTCCTCTCTCATGCAATTTCTATGGCTTTATGTTCTGCTCTTTTGGACCTCTGTCTTCAAACCTTGTTACATCACACCCATTTTGTTTGAACTTCTCTATAGCTGTTTTTGATGAAGTTAGTAATTGAGATATTTGAGCTCGGCTGGTGGTTGTTGTCGTGCAGAATATTTGTGTAACAACTCTGTTCTATAATCTTTTGAAGGATGTGGGGATTTTCTATTTGTAATCTATTTCATATATCAATGAGATGGTTCTGTTTCCTATATATATATAAAAAAAGGAACTGAAGAGGTGGTTTGTTTGATTCTTTAAGACAACCCTTGATAAATCATGTTTTTCAAACGTTTATTTGTTATTTTATTTGTTTGGGGGTTCTTGCATTTACTTAATTTGTTTATTATTAGATCTGAAACATGTTCTGTTTTCATATTTTATAATGAAGATGCAAGCATTGTGATTTAATTTTCAATGTTGCAGAAGATGCTCTATTTGATGCTTCGCAGTAAGCATTTTTTGGCAAGGATATGGAGGAGGTTGAATTGGGGGGATTAGAAGATGAAGAGGATGATGCACTGTTATTTCCTTTATTTGATAAACAGGTATCTGTTATTTCCTTTATTCTATATAGTTCTGTTCCTCTTTTTAGCTGGATTAGTGGATGCACTTGTTTTCATTTTAAAAAACGTTGGTCGTTGCATGTGGTTGACTAGGAGTCAGTATTGAATTTTCCATGGGAGCTTTTTGTTTTCTCAATGAGCAGGACAAAACACTTTGGACTAATGCCTCTTTTGTTATTTAACGGTAGATTTGGGTGGTTAAAATAGATTTTTTTTTTTAATTTTTTTTTTACATTTCTTGGGGACTGAAAGAGAGTCGGCATCATGTTATTTTTTTGGAATATAAATGATAAAATCTTTGGTCATTTGACAATGTGCAGTTGTGTGTTTGTATGCATGTGTGTTTCGTGATTTTCGAATGCTGTTGGGCCGGACCCTTGTCACCCCCTGTACATGTTTACCAATCGACATAGTTTTTTCTTATATATGTCTGTTAACTTGTAAGTCTATGGGAAATAATATTCTGCCTGTAAGAAAACTGGAAAATAAGGCTAGCCACGTTCAAACACAATGTTTATTTGATTGTTTGTCCTTCAATATCTAAATTTTTAGCCTAAATGTGCAGGCCCCTCTTTTGATTGGTTGTGATGTTCGAAACATGACTAAATAAACTTTAGAACTTCTTAGCAATGAGGAGGTCATTGCAGTAAACCAAGGTGAACCGCACTTTGTTCAACACGCTTAGTTATGTTTTCGTCTTTTTGATGTTATATATTCACATGAATGAAATGTCCATGAGTATAATTCATGTTTATTGATATCAGTAAGTTTGAATTATTTCACTGAGTGAGATAATCTACAATATGCTTAAATTAATTATTTATTCATCAAGGGCGAAATTAATATATTAATATTTATTTTATGCAGATCCTGTTGGGGTCCAGGGAAGAAAGGTTAAAGTTTTTGGAAAGGATGGTTGCCTTCAGGTATTCCAGTTTTTGCATTACCTTATAGATTATATTAGCAAGATGACACTGTGTGACATACAAAGATGGCAATATTTACTTGTATTTGATGCAGAATAATGTAAAATGTATAACTGGAAGCATCTTTACCTTGCAAACTTGATGTTAGTGTTCAAGTGAAAACTGAGAAGGCAGATTATTATCTGACTATTTGATTTTGGTTACTATTGTGGTTTGCACCTAATTAACCAAGTATATCAGTTAGCTATTGAATGTTTCAGTATATAAAGGGTTTGTTTTACAGGTTTGGGCAGGTCCTCTATCTGGAAGCCGCTTGGCGGTCATTCTTTGGAACCGATGTTCAGTTGCATCAACGATCACCACGGATTGGAACGCACTGGGGCTTAAACCCGGCACCAGTGTCTCAGTAAGGGACTTGTGGCAGGTAAGGAATCTGGCTCCTTTAAATTATTTGGTGTTGTTACAGTTACAGCCTCTAGACCTGATCTCTTGA

mRNA sequence

ATGGATGGTTTCGGTAACGGACCTAGACTTCAAGTTGCGTCTACATCCGATCCGATGATCTCACGCGTTTTCGAGCCAATTCCACGGAAGATGCTCTATTTGATGCTTCGCAATCCTGTTGGGGTCCAGGGAAGAAAGGTTAAAGTTTTTGGAAAGGATGGTTGCCTTCAGGTTTGGGCAGGTCCTCTATCTGGAAGCCGCTTGGCGGTCATTCTTTGGAACCGATGTTCAGTTGCATCAACGATCACCACGGATTGGAACGCACTGGGGCTTAAACCCGGCACCAGTGTCTCAGTAAGGGACTTGTGGCAGTTACAGCCTCTAGACCTGATCTCTTGA

Coding sequence (CDS)

ATGGATGGTTTCGGTAACGGACCTAGACTTCAAGTTGCGTCTACATCCGATCCGATGATCTCACGCGTTTTCGAGCCAATTCCACGGAAGATGCTCTATTTGATGCTTCGCAATCCTGTTGGGGTCCAGGGAAGAAAGGTTAAAGTTTTTGGAAAGGATGGTTGCCTTCAGGTTTGGGCAGGTCCTCTATCTGGAAGCCGCTTGGCGGTCATTCTTTGGAACCGATGTTCAGTTGCATCAACGATCACCACGGATTGGAACGCACTGGGGCTTAAACCCGGCACCAGTGTCTCAGTAAGGGACTTGTGGCAGTTACAGCCTCTAGACCTGATCTCTTGA

Protein sequence

MDGFGNGPRLQVASTSDPMISRVFEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSVASTITTDWNALGLKPGTSVSVRDLWQLQPLDLIS

Homology

[Close]

BLAST of Tan0020673 vs. ExPASy Swiss-Prot
Match: Q8VXZ7 (Alpha-galactosidase 3 OS=Arabidopsis thaliana OX=3702 GN=AGAL3 PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 2.8e-20
Identity = 43/86 (50.00%), Postives = 60/86 (69.77%), Query Frame = 0


Query: 19  MISRVFEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSV 78
           M +   E +  K +  + ++P+GVQGRK++  G++ C QVW+GPLSG R+ V LWNRCS 
Sbjct: 316 MTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGDRMVVALWNRCSE 375

Query: 79  ASTITTDWNALGLKPGTSVSVRDLWQ 105
            +TIT  W+ +GL+   SVSVRDLWQ
Sbjct: 376 PATITASWDMIGLESTISVSVRDLWQ 401

[Close]

BLAST of Tan0020673 vs. ExPASy Swiss-Prot
Match: P14749 (Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 1.0e-14
Identity = 37/76 (48.68%), Postives = 53/76 (69.74%), Query Frame = 0


Query: 37  RNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSVASTITTDWNALGLKPGTS 96
           ++ +GVQG+KVK       L+VWAGPLS +++AVILWNR S  +T+T  W+ +GL+ GT+
Sbjct: 318 QDKLGVQGKKVK---STNDLEVWAGPLSDNKVAVILWNRSSSRATVTASWSDIGLQQGTT 377

Query: 97  VSVRDLWQLQPLDLIS 113
           V  RDLW+     L+S
Sbjct: 378 VDARDLWEHSTQSLVS 390

[Close]

BLAST of Tan0020673 vs. ExPASy Swiss-Prot
Match: Q8RX86 (Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1)

HSP 1 Score: 79.3 bits (194), Expect = 3.0e-14
Identity = 38/81 (46.91%), Postives = 54/81 (66.67%), Query Frame = 0


Query: 24  FEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSVASTIT 83
           FE +  K +  + ++ +G+QG+KVK   K+G L+VWAGPLS  R+AVILWNR S ++ IT
Sbjct: 289 FELLSNKEVIAVNQDKLGIQGKKVK---KEGDLEVWAGPLSKKRVAVILWNRGSASANIT 348

Query: 84  TDWNALGLKPGTSVSVRDLWQ 105
             W  +GL     V+ RDLW+
Sbjct: 349 ARWAEIGLNSSDIVNARDLWE 366

[Close]

BLAST of Tan0020673 vs. ExPASy Swiss-Prot
Match: Q42656 (Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 5.6e-13
Identity = 38/85 (44.71%), Postives = 52/85 (61.18%), Query Frame = 0


Query: 19  MISRVFEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSV 78
           M    F+ +    +  + ++ +GVQG KVK +G    L+VWAGPLSG R+AV LWNR S 
Sbjct: 268 MDGATFQLLSNAEVIAVNQDKLGVQGNKVKTYGD---LEVWAGPLSGKRVAVALWNRGSS 327

Query: 79  ASTITTDWNALGLKPGTSVSVRDLW 104
            +TIT  W+ +GL     V+ RDLW
Sbjct: 328 TATITAYWSDVGLPSTAVVNARDLW 349

[Close]

BLAST of Tan0020673 vs. ExPASy Swiss-Prot
Match: Q9FXT4 (Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.3e-12
Identity = 34/67 (50.75%), Postives = 47/67 (70.15%), Query Frame = 0


Query: 37  RNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSVASTITTDWNALGLKPGTS 96
           ++ +GVQG+KV+    D  L+VWAGPLS +R AV+LWNR S  +TIT  W+ +GL    +
Sbjct: 326 QDSLGVQGKKVQ---SDNGLEVWAGPLSNNRKAVVLWNRQSYQATITAHWSNIGLAGSVA 385

Query: 97  VSVRDLW 104
           V+ RDLW
Sbjct: 386 VTARDLW 389

[Close]

BLAST of Tan0020673 vs. NCBI nr
Match: XP_022943114.1 (alpha-galactosidase 3 [Cucurbita moschata] >XP_022943115.1 alpha-galactosidase 3 [Cucurbita moschata] >XP_022943116.1 alpha-galactosidase 3 [Cucurbita moschata])

HSP 1 Score: 141.7 bits (356), Expect = 3.7e-30
Identity = 65/85 (76.47%), Postives = 73/85 (85.88%), Query Frame = 0


Query: 19  MISRVFEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSV 78
           M    FE +  + +  + ++P+GVQGRKVKVFGKDGCLQVWAGPLSGSRLAV+LWNRCSV
Sbjct: 309 MTKETFEILSNEEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVVLWNRCSV 368

Query: 79  ASTITTDWNALGLKPGTSVSVRDLW 104
           ASTITTDWN LGLKPGTSVSVRDLW
Sbjct: 369 ASTITTDWNTLGLKPGTSVSVRDLW 393

[Close]

BLAST of Tan0020673 vs. NCBI nr
Match: XP_022985233.1 (alpha-galactosidase 3 [Cucurbita maxima] >XP_022985242.1 alpha-galactosidase 3 [Cucurbita maxima] >XP_022985247.1 alpha-galactosidase 3 [Cucurbita maxima])

HSP 1 Score: 141.7 bits (356), Expect = 3.7e-30
Identity = 65/85 (76.47%), Postives = 73/85 (85.88%), Query Frame = 0


Query: 19  MISRVFEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSV 78
           M    FE +  + +  + ++P+GVQGRKVKVFGKDGCLQVWAGPLSGSRLAV+LWNRCSV
Sbjct: 309 MTKETFEILSNEEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVVLWNRCSV 368

Query: 79  ASTITTDWNALGLKPGTSVSVRDLW 104
           ASTITTDWN LGLKPGTSVSVRDLW
Sbjct: 369 ASTITTDWNTLGLKPGTSVSVRDLW 393

[Close]

BLAST of Tan0020673 vs. NCBI nr
Match: KAG6600883.1 (Alpha-galactosidase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 141.7 bits (356), Expect = 3.7e-30
Identity = 65/85 (76.47%), Postives = 73/85 (85.88%), Query Frame = 0


Query: 19  MISRVFEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSV 78
           M    FE +  + +  + ++P+GVQGRKVKVFGKDGCLQVWAGPLSGSRLAV+LWNRCSV
Sbjct: 309 MTKETFEILSNEEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVVLWNRCSV 368

Query: 79  ASTITTDWNALGLKPGTSVSVRDLW 104
           ASTITTDWN LGLKPGTSVSVRDLW
Sbjct: 369 ASTITTDWNTLGLKPGTSVSVRDLW 393

[Close]

BLAST of Tan0020673 vs. NCBI nr
Match: KGN50728.1 (hypothetical protein Csa_005887 [Cucumis sativus])

HSP 1 Score: 139.0 bits (349), Expect = 2.4e-29
Identity = 65/85 (76.47%), Postives = 72/85 (84.71%), Query Frame = 0


Query: 19  MISRVFEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSV 78
           M     E +  K +  + ++P+GVQGRKVKVFGKDGCLQVWAGPLSGSRLAV+LWNRCSV
Sbjct: 309 MTKETSEILMNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVVLWNRCSV 368

Query: 79  ASTITTDWNALGLKPGTSVSVRDLW 104
           ASTITTDWNALGLKP TSVSVRDLW
Sbjct: 369 ASTITTDWNALGLKPNTSVSVRDLW 393

[Close]

BLAST of Tan0020673 vs. NCBI nr
Match: NP_001292680.1 (alpha-galactosidase 3 precursor [Cucumis sativus] >AEQ94270.1 alpha galacdosidase 3 [Cucumis sativus])

HSP 1 Score: 138.7 bits (348), Expect = 3.2e-29
Identity = 64/85 (75.29%), Postives = 72/85 (84.71%), Query Frame = 0


Query: 19  MISRVFEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSV 78
           M     E +  K +  + ++P+GVQGRKVKVFGKDGCLQ+WAGPLSGSRLAV+LWNRCSV
Sbjct: 309 MTKETSEILMNKEVIAVNQDPLGVQGRKVKVFGKDGCLQIWAGPLSGSRLAVVLWNRCSV 368

Query: 79  ASTITTDWNALGLKPGTSVSVRDLW 104
           ASTITTDWNALGLKP TSVSVRDLW
Sbjct: 369 ASTITTDWNALGLKPNTSVSVRDLW 393

[Close]

BLAST of Tan0020673 vs. ExPASy TrEMBL
Match: A0A6J1J4C9 (Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111483287 PE=3 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.8e-30
Identity = 65/85 (76.47%), Postives = 73/85 (85.88%), Query Frame = 0


Query: 19  MISRVFEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSV 78
           M    FE +  + +  + ++P+GVQGRKVKVFGKDGCLQVWAGPLSGSRLAV+LWNRCSV
Sbjct: 309 MTKETFEILSNEEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVVLWNRCSV 368

Query: 79  ASTITTDWNALGLKPGTSVSVRDLW 104
           ASTITTDWN LGLKPGTSVSVRDLW
Sbjct: 369 ASTITTDWNTLGLKPGTSVSVRDLW 393

[Close]

BLAST of Tan0020673 vs. ExPASy TrEMBL
Match: A0A6J1FQU5 (Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111447942 PE=3 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 1.8e-30
Identity = 65/85 (76.47%), Postives = 73/85 (85.88%), Query Frame = 0


Query: 19  MISRVFEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSV 78
           M    FE +  + +  + ++P+GVQGRKVKVFGKDGCLQVWAGPLSGSRLAV+LWNRCSV
Sbjct: 309 MTKETFEILSNEEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVVLWNRCSV 368

Query: 79  ASTITTDWNALGLKPGTSVSVRDLW 104
           ASTITTDWN LGLKPGTSVSVRDLW
Sbjct: 369 ASTITTDWNTLGLKPGTSVSVRDLW 393

[Close]

BLAST of Tan0020673 vs. ExPASy TrEMBL
Match: A0A0A0KM98 (Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_5G220910 PE=3 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 1.2e-29
Identity = 65/85 (76.47%), Postives = 72/85 (84.71%), Query Frame = 0


Query: 19  MISRVFEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSV 78
           M     E +  K +  + ++P+GVQGRKVKVFGKDGCLQVWAGPLSGSRLAV+LWNRCSV
Sbjct: 309 MTKETSEILMNKEVIAVNQDPLGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVVLWNRCSV 368

Query: 79  ASTITTDWNALGLKPGTSVSVRDLW 104
           ASTITTDWNALGLKP TSVSVRDLW
Sbjct: 369 ASTITTDWNALGLKPNTSVSVRDLW 393

[Close]

BLAST of Tan0020673 vs. ExPASy TrEMBL
Match: G8I091 (Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=GAL3 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.5e-29
Identity = 64/85 (75.29%), Postives = 72/85 (84.71%), Query Frame = 0


Query: 19  MISRVFEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSV 78
           M     E +  K +  + ++P+GVQGRKVKVFGKDGCLQ+WAGPLSGSRLAV+LWNRCSV
Sbjct: 309 MTKETSEILMNKEVIAVNQDPLGVQGRKVKVFGKDGCLQIWAGPLSGSRLAVVLWNRCSV 368

Query: 79  ASTITTDWNALGLKPGTSVSVRDLW 104
           ASTITTDWNALGLKP TSVSVRDLW
Sbjct: 369 ASTITTDWNALGLKPNTSVSVRDLW 393

[Close]

BLAST of Tan0020673 vs. ExPASy TrEMBL
Match: A0A6J1CZ35 (Alpha-galactosidase OS=Momordica charantia OX=3673 GN=LOC111015913 PE=3 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 7.6e-29
Identity = 63/86 (73.26%), Postives = 71/86 (82.56%), Query Frame = 0


Query: 19  MISRVFEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSV 78
           M     E +  + +  + ++P GVQGRKVKV GKDGCL+VWAGPLSGSRLAV+LWNRCSV
Sbjct: 309 MTEETLEILSNEEVIAVNQDPFGVQGRKVKVLGKDGCLEVWAGPLSGSRLAVVLWNRCSV 368

Query: 79  ASTITTDWNALGLKPGTSVSVRDLWQ 105
           ASTITTDWNALGLKP TSVSVRDLWQ
Sbjct: 369 ASTITTDWNALGLKPDTSVSVRDLWQ 394

[Close]

BLAST of Tan0020673 vs. TAIR 10
Match: AT3G56310.2 (Melibiase family protein )

HSP 1 Score: 99.4 bits (246), Expect = 2.0e-21
Identity = 43/86 (50.00%), Postives = 60/86 (69.77%), Query Frame = 0


Query: 19  MISRVFEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSV 78
           M +   E +  K +  + ++P+GVQGRK++  G++ C QVW+GPLSG R+ V LWNRCS 
Sbjct: 292 MTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGDRMVVALWNRCSE 351

Query: 79  ASTITTDWNALGLKPGTSVSVRDLWQ 105
            +TIT  W+ +GL+   SVSVRDLWQ
Sbjct: 352 PATITASWDMIGLESTISVSVRDLWQ 377

[Close]

BLAST of Tan0020673 vs. TAIR 10
Match: AT3G56310.1 (Melibiase family protein )

HSP 1 Score: 99.4 bits (246), Expect = 2.0e-21
Identity = 43/86 (50.00%), Postives = 60/86 (69.77%), Query Frame = 0


Query: 19  MISRVFEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSV 78
           M +   E +  K +  + ++P+GVQGRK++  G++ C QVW+GPLSG R+ V LWNRCS 
Sbjct: 316 MTAETLEILSNKEIIAVNQDPLGVQGRKIQANGENDCQQVWSGPLSGDRMVVALWNRCSE 375

Query: 79  ASTITTDWNALGLKPGTSVSVRDLWQ 105
            +TIT  W+ +GL+   SVSVRDLWQ
Sbjct: 376 PATITASWDMIGLESTISVSVRDLWQ 401

[Close]

BLAST of Tan0020673 vs. TAIR 10
Match: AT5G08370.1 (alpha-galactosidase 2 )

HSP 1 Score: 79.3 bits (194), Expect = 2.1e-15
Identity = 38/81 (46.91%), Postives = 54/81 (66.67%), Query Frame = 0


Query: 24  FEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSVASTIT 83
           FE +  K +  + ++ +G+QG+KVK   K+G L+VWAGPLS  R+AVILWNR S ++ IT
Sbjct: 289 FELLSNKEVIAVNQDKLGIQGKKVK---KEGDLEVWAGPLSKKRVAVILWNRGSASANIT 348

Query: 84  TDWNALGLKPGTSVSVRDLWQ 105
             W  +GL     V+ RDLW+
Sbjct: 349 ARWAEIGLNSSDIVNARDLWE 366

[Close]

BLAST of Tan0020673 vs. TAIR 10
Match: AT5G08370.2 (alpha-galactosidase 2 )

HSP 1 Score: 79.3 bits (194), Expect = 2.1e-15
Identity = 38/81 (46.91%), Postives = 54/81 (66.67%), Query Frame = 0


Query: 24  FEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSVASTIT 83
           FE +  K +  + ++ +G+QG+KVK   K+G L+VWAGPLS  R+AVILWNR S ++ IT
Sbjct: 263 FELLSNKEVIAVNQDKLGIQGKKVK---KEGDLEVWAGPLSKKRVAVILWNRGSASANIT 322

Query: 84  TDWNALGLKPGTSVSVRDLWQ 105
             W  +GL     V+ RDLW+
Sbjct: 323 ARWAEIGLNSSDIVNARDLWE 340

[Close]

BLAST of Tan0020673 vs. TAIR 10
Match: AT5G08380.1 (alpha-galactosidase 1 )

HSP 1 Score: 65.9 bits (159), Expect = 2.4e-11
Identity = 34/90 (37.78%), Postives = 53/90 (58.89%), Query Frame = 0


Query: 19  MISRVFEPIPRKMLYLMLRNPVGVQGRKVKVFGKDGCLQVWAGPLSGSRLAVILWNRCSV 78
           M     E +  K +  + ++P GVQ +KV++   +G L+VWAGPLSG R+A++L NR   
Sbjct: 298 MTKETMEIVANKEVIAINQDPHGVQAKKVRM---EGDLEVWAGPLSGYRVALLLLNRGPS 357

Query: 79  ASTITTDWNALGLKPGTSVSVRDLWQLQPL 109
            ++IT  W  + +   + V  RDLW+ Q L
Sbjct: 358 RTSITALWEDIEIPANSIVEARDLWEHQTL 384

The following BLAST results are available for this feature:

BLAST of Tan0020673 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VXZ7	2.8e-20	50.00	Alpha-galactosidase 3 OS=Arabidopsis thaliana OX=3702 GN=AGAL3 PE=1 SV=1	[more]
P14749	1.0e-14	48.68	Alpha-galactosidase OS=Cyamopsis tetragonoloba OX=3832 PE=1 SV=1	[more]
Q8RX86	3.0e-14	46.91	Alpha-galactosidase 2 OS=Arabidopsis thaliana OX=3702 GN=AGAL2 PE=1 SV=1	[more]
Q42656	5.6e-13	44.71	Alpha-galactosidase OS=Coffea arabica OX=13443 PE=1 SV=1	[more]
Q9FXT4	1.3e-12	50.75	Alpha-galactosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os10g0493600 PE=...	[more]

BLAST of Tan0020673 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022943114.1	3.7e-30	76.47	alpha-galactosidase 3 [Cucurbita moschata] >XP_022943115.1 alpha-galactosidase 3...	[more]
XP_022985233.1	3.7e-30	76.47	alpha-galactosidase 3 [Cucurbita maxima] >XP_022985242.1 alpha-galactosidase 3 [...	[more]
KAG6600883.1	3.7e-30	76.47	Alpha-galactosidase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KGN50728.1	2.4e-29	76.47	hypothetical protein Csa_005887 [Cucumis sativus]	[more]
NP_001292680.1	3.2e-29	75.29	alpha-galactosidase 3 precursor [Cucumis sativus] >AEQ94270.1 alpha galacdosidas...	[more]

BLAST of Tan0020673 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J4C9	1.8e-30	76.47	Alpha-galactosidase OS=Cucurbita maxima OX=3661 GN=LOC111483287 PE=3 SV=1	[more]
A0A6J1FQU5	1.8e-30	76.47	Alpha-galactosidase OS=Cucurbita moschata OX=3662 GN=LOC111447942 PE=3 SV=1	[more]
A0A0A0KM98	1.2e-29	76.47	Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=Csa_5G220910 PE=3 SV=1	[more]
G8I091	1.5e-29	75.29	Alpha-galactosidase OS=Cucumis sativus OX=3659 GN=GAL3 PE=2 SV=1	[more]
A0A6J1CZ35	7.6e-29	73.26	Alpha-galactosidase OS=Momordica charantia OX=3673 GN=LOC111015913 PE=3 SV=1	[more]

BLAST of Tan0020673 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56310.2	2.0e-21	50.00	Melibiase family protein	[more]
AT3G56310.1	2.0e-21	50.00	Melibiase family protein	[more]
AT5G08370.1	2.1e-15	46.91	alpha-galactosidase 2	[more]
AT5G08370.2	2.1e-15	46.91	alpha-galactosidase 2	[more]
AT5G08380.1	2.4e-11	37.78	alpha-galactosidase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	2.60.40.1180		coord: 41..111 e-value: 7.1E-20 score: 72.8
IPR041233	Alpha galactosidase, C-terminal beta sandwich domain	PFAM	PF17801	Melibiase_C	coord: 53..104 e-value: 5.2E-14 score: 52.0
IPR002241	Glycoside hydrolase, family 27	PANTHER	PTHR11452	ALPHA-GALACTOSIDASE/ALPHA-N-ACETYLGALACTOSAMINIDASE	coord: 37..104
None	No IPR available	PANTHER	PTHR11452:SF75	ALPHA-GALACTOSIDASE 3	coord: 37..104
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 49..108

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0020673.1	Tan0020673.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0009965	leaf morphogenesis
biological_process	GO:0009911	positive regulation of flower development
biological_process	GO:0009620	response to fungus
cellular_component	GO:0048046	apoplast
cellular_component	GO:0009505	plant-type cell wall
molecular_function	GO:0052692	raffinose alpha-galactosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds