Tan0020546 (gene) Snake gourd v1

BLAST of Tan0020546 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.6e-100
Identity = 226/468 (48.29%), Postives = 291/468 (62.18%), Query Frame = 0

Query: 7   SLSLSLFLILVAVAPFGSYS-VRID--FSIEEATVKDLQLAFNQKKLTSRQLVEFYIEQI 66
           SL + L L    +   GS S +R+   FSI+EAT++D+++AFN+K+LTS+QLVE Y+E I
Sbjct: 11  SLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAI 70

Query: 67  RRYNPRLRGVMEVNPDALHLANKADRKRQEKAPGSVPLSHGIPVLVKDNIATKGKLNTIA 126
            + NP L  V+E NPDAL  A  ADR+R  K    +P+ HG+PVL+KD+I+TK KLNT A
Sbjct: 71  SKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNTTA 130

Query: 127 GSFALLGSIVPRDTGVVKKLRKVGAIILGKASLSEWSSFRSHKAPPDWNAR--------- 186
           GSFALLGS+V RD GVVK+LR+ GA+ILGKASLSEW+ FRS   P  W+AR         
Sbjct: 131 GSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSARGLQGKNPYV 190

Query: 187 ----------------------MGQGTETDGSILCPSSFSSVVGIKRIIGLTSRAGVVPI 246
                                 +  GTETDGSIL P+S +SVVGIK  +GLTSRAGVVPI
Sbjct: 191 LSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVGLTSRAGVVPI 250

Query: 247 SPQQDTVG-----------------------------FSYIPRGGYSPFLKADGLRGKRL 306
           S +QD++G                               +IP GGY  FL   GL+GKRL
Sbjct: 251 SLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGKRL 310

Query: 307 GIVDGFFGGGPSISLAFEKILIILRKRGAILVNNLKIENLKTIISNHTASGEVTALRNEF 366
           GIV          S   +  +  LR+ GAI++NNL I N++ I+   T SGE  AL  EF
Sbjct: 311 GIV-------MKHSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGG-TDSGEEIALLAEF 370

Query: 367 KISLNAYLKELVSSPIRSLSDSIAFNQKHADLEKIKEYGQELF----RRSRDDERNRNGG 407
           K+SLNAYLKELV SP+RSL+D IA+N++ A+ EK+KE+GQE+F      S   E+ +   
Sbjct: 371 KMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTAL 430

BLAST of Tan0020546 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.9e-42
Identity = 126/381 (33.07%), Postives = 189/381 (49.61%), Query Frame = 0

Query: 30  DFSIEEATVKDLQLAFNQKKLTSRQLVEFYIEQIRRYNPRLRGVMEVNPDALHLANKADR 89
           + ++E+AT+  LQ       LTS  +V  Y+++  + NP + G++++NPD L +A++ D 
Sbjct: 63  NITLEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELD- 122

Query: 90  KRQEKAPGSV--PLSHGIPVLVKDNIATKGKLNTIAGSFALLGSIVPRDTGVVKKLRKVG 149
              E+A G +  PL HGIP +VKDN ATK K++T AGS+ALLGSIVPRD  VVK+LR+ G
Sbjct: 123 --DERANGIIRGPL-HGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAG 182

Query: 150 AIILGKASLSEWSSFRSHKAPPDWNARMGQ------------------------------ 209
           A++ G A+LSEW+  RS+     ++AR GQ                              
Sbjct: 183 AVLFGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFA 242

Query: 210 -GTETDGSILCPSSFSSVVGIKRIIGLTSRAGVVPISPQQDTVG--------FSYI---- 269
            GTETDGSI+ P+  + VVG+K  +GLTSR GV+P S  QDT G          Y+    
Sbjct: 243 LGTETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSM 302

Query: 270 ------------------PRGGYSPFL-KADGLRGKRLGIVDGFFGGGPSISLAFEKILI 329
                               G Y  FL     L G R G+               +++L 
Sbjct: 303 WGIDENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRLWQNAKAD-EIDRLLE 362

Query: 330 ILR---KRGAILVNNLKIENLKTIISN-------HTASGEVTALRNEFKISLNAYLKELV 337
           +++   + GAI+ NN    NL  I ++            E T ++ +F  ++ +YL E+ 
Sbjct: 363 VVKQIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVK 422

BLAST of Tan0020546 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 7.9e-36
Identity = 134/441 (30.39%), Postives = 204/441 (46.26%), Query Frame = 0

Query: 41  LQLAFNQKKLTSRQLVEFYIEQIRRYNPRLRGVMEVNPDALHLANKADRKRQ-EKAPGSV 100
           LQ  + Q  +    +V+ Y+ +I   N  +R V E+NPDAL +A + D +R+  K  G  
Sbjct: 94  LQTPYRQGNVLIIVVVQTYVARIAEVNSTVRAVTEINPDALTIAKQMDNERKMGKLRG-- 153

Query: 101 PLSHGIPVLVKDNIATKGKLNTIAGSFALLGSIVPRDTGVVKKLRKVGAIILGKASLSEW 160
           PL HG+P+++K+NI T  K+++ AGS+A+ G+    D  V  KLR+ G +I+GK+  S+W
Sbjct: 154 PL-HGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATKLREAGLVIMGKSGASQW 213

Query: 161 SSFRSHKAPPDWNARMGQ-------------------------------GTETDGSILCP 220
           ++FRS  +   W+A  GQ                               GTET GSI+ P
Sbjct: 214 ANFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLGLAFATLGTETSGSIVSP 273

Query: 221 SSFSSVVGIKRIIGLTSRAGVVPISPQQDTVG-------------------------FSY 280
           +  S++VG+K  +GLTSR  VVPIS +QDTVG                          S 
Sbjct: 274 ADKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAYLLQVIAGKDSNDNYTSA 333

Query: 281 IPRGGYSPFLKA---DGLRGKRLGI---VDGFFGGGPSISLAFEKILIILRKRGAILVNN 340
           IP      ++KA   + L+GKR+G+   V   FG   ++   F + L +++K GAI+V N
Sbjct: 334 IPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTVVDQFNQALAVMKKAGAIIVEN 393

Query: 341 LKIENLKTIISNHTASGEVTALRNEFKISLNAYLKELVSSP--IRSLSDSIAFNQKHADL 400
               +      +      + A   +   +L A+ K+L  +P  I  L     F Q H   
Sbjct: 394 TDFTSFAEFAQSPIPDDILYA---DSLTNLPAFFKQLKVNPHNITDLESLRRFTQHH--- 453

Query: 401 EKIKEYGQELFRRSRDDERNRNGGSDIKI-----------SEDGIEKTITENKLDAIVTS 406
            +++EY      R     +     +D K            +E GI   +  +KLDA V  
Sbjct: 454 -RLEEYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGNEGGILGALRRHKLDAAVLP 513

BLAST of Tan0020546 vs. ExPASy Swiss-Prot
Match: A6TTJ8 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Alkaliphilus metalliredigens (strain QYMF) OX=293826 GN=gatA PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 2.5e-18
Identity = 82/296 (27.70%), Postives = 134/296 (45.27%), Query Frame = 0

Query: 33  IEEATVKDLQLAFNQKKLTSRQLVEFYIEQIRRYNPRLRGVMEV-NPDALHLANKADRKR 92
           IEE T+ D++  + QK+ T + +V+ YI++I+  + ++   + +    AL  A   D K 
Sbjct: 3   IEEMTIMDIRKGYVQKQFTVKDVVQGYIDRIKELDGKINAFITLCEESALMEAAVLDEKL 62

Query: 93  QEKAPGSVPLSHGIPVLVKDNIATKGKLNTIAGSFALLGSIVPRDTGVVKKLRKVGAIIL 152
                  + L  GIPV +KDN+ T+G + T   S  L   I P D  +VKKLR  GAII+
Sbjct: 63  SRGE--EIGLLGGIPVAIKDNMCTQG-IKTSCASEMLADFIPPYDATIVKKLRAAGAIII 122

Query: 153 GKASLSEW--------SSFRSHKAPPDWN------------------ARMGQGTETDGSI 212
           GK ++ E+        S+F+  K P D                    A +  G++T GSI
Sbjct: 123 GKTNMDEFAMGSSTENSAFKVTKNPWDLTKVPGGSSGGSAAALAAGFAPLTIGSDTGGSI 182

Query: 213 LCPSSFSSVVGIKRIIGLTSRAGVVPISPQQDTVG----------FSYIPRGGYSPFLKA 272
             P++F   VG+K   GL SR G++  +   D +G           S     G  P    
Sbjct: 183 RQPAAFCGTVGLKPTYGLVSRFGLIAFASSLDQIGPFTKTVKDCALSLQVMQGNDPLDST 242

Query: 273 D---------------GLRGKRLGIVDGFFGGGPSISL--AFEKILIILRKRGAIL 275
                           G++G ++GI   FF  G +I +  + ++ + +L + GA++
Sbjct: 243 SIQQEPMDDYVKDLDKGVKGLKVGIPKEFFQEGLNIEISDSIKEAIKVLEQLGAVV 295

BLAST of Tan0020546 vs. ExPASy Swiss-Prot
Match: B8HY89 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) OX=395961 GN=gatA PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.6e-17
Identity = 78/291 (26.80%), Postives = 132/291 (45.36%), Query Frame = 0

Query: 36  ATVKDLQLAFNQKKLTSRQLVEFYIEQIRRYNPRLRGVMEVNPDALHLANKADRKRQEKA 95
           A++++L      K+ +++++ +  +E+I++  P++   + +   A     +A+R  Q+ A
Sbjct: 2   ASIRELHQQLVSKERSAKEITQDALEKIQQLEPKVHAFLTLT--AEQALAQAERVDQQIA 61

Query: 96  PGS-VPLSHGIPVLVKDNIATKGKLNTIAGSFALLGSIVPRDTGVVKKLRKVGAIILGKA 155
            G+ + L  GIP+ +KDN+ TKG + T  GS  L G I P ++ V  +L   GA+++GK 
Sbjct: 62  TGTEIGLLAGIPIAIKDNLCTKG-IPTTCGSKILQGFIPPYESTVTSRLAAAGAVMVGKT 121

Query: 156 SLSEW--------------------------SSFRSHKAPPDWNARMGQGTETDGSILCP 215
           +L E+                          SS  S  A       +  G++T GSI  P
Sbjct: 122 NLDEFAMGSSTENSAYQLTANPWDLQRVPGGSSGGSAAAVAAGETLIALGSDTGGSIRQP 181

Query: 216 SSFSSVVGIKRIIGLTSRAGVVPISPQQDTVG-------------------------FSY 273
           +SF  VVG+K   GL SR G+V  +   D +G                            
Sbjct: 182 ASFCGVVGLKPTYGLVSRYGLVAYASSLDQIGPFATNVEDAALLLGAIAGHDPQDSTSLN 241

BLAST of Tan0020546 vs. NCBI nr
Match: XP_022146229.1 (putative amidase C869.01 [Momordica charantia])

HSP 1 Score: 527.3 bits (1357), Expect = 1.2e-145
Identity = 304/473 (64.27%), Postives = 346/473 (73.15%), Query Frame = 0

Query: 1   MEAPSFSLSLSLFLILVAVAPFGSYSVR-IDFSIEEATVKDLQLAFNQKKLTSRQLVEFY 60
           ME P  SLS SLFLILVAV+PF SYSVR   F IEEA +KDL LAF Q KLTSRQLVEFY
Sbjct: 1   METPRLSLSFSLFLILVAVSPFWSYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEFY 60

Query: 61  IEQIRRYNPRLRGVMEVNPDALHLANKADRKRQEKAPGSVPLSHGIPVLVKDNIATKGKL 120
           I+QIRRYNPRLRGV+EVNPDAL+LA+KADR+R+ KAPGS+PL HGIPVLVKDN+ATK KL
Sbjct: 61  IKQIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKL 120

Query: 121 NTIAGSFALLGSIVPRDTGVVKKLRKVGAIILGKASLSEWSSFRSHKAPPDWNARMGQ-- 180
           NT AGS ALLGS+VPRD G V +LRK GAIILGKAS+SEW+ FRS+ AP  WNAR GQ  
Sbjct: 121 NTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGR 180

Query: 181 ----------------------------GTETDGSILCPSSFSSVVGIKRIIGLTSRAGV 240
                                       GTETDGSILCPSSF+SVVGIK  +GLTS AGV
Sbjct: 181 EPYTLGLPCGSSSGSAISVSANMAAVALGTETDGSILCPSSFNSVVGIKPTLGLTSTAGV 240

Query: 241 VPISPQQDTVG-----------------------------FSYIPRGGYSPFLKADGLRG 300
           VPISP+QDTVG                               YIPRGGY  FLKADGLRG
Sbjct: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRG 300

Query: 301 KRLGIVDGFFG-GGPSISLAFEKILIILRKRGAILVNNLKIENLKTIISNHTASGEVTAL 360
           KRLGIV+ FFG   PS+  AFE+I  IL KRGAILV+NLKI+NL  I  N+T SGEV AL
Sbjct: 301 KRLGIVEDFFGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIY-NNTESGEVAAL 360

Query: 361 RNEFKISLNAYLKELVSSPIRSLSDSIAFNQKHADLEKIKEYGQELFRRSRDDERNRNGG 408
            NEFK+SLNAYLKELVSSPIRSL+++IAFNQKH+ LEKIKEYGQ+LF ++  +  N  G 
Sbjct: 361 LNEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQA--EATNGMGI 420

BLAST of Tan0020546 vs. NCBI nr
Match: KAF8410439.1 (hypothetical protein HHK36_002968 [Tetracentron sinense])

HSP 1 Score: 427.6 bits (1098), Expect = 1.3e-115
Identity = 249/474 (52.53%), Postives = 311/474 (65.61%), Query Frame = 0

Query: 8   LSLSLFLILVAVAPFGSYSVRIDFSIEEATVKDLQLAFNQKKLTSRQLVEFYIEQIRRYN 67
           L LS  L+++ ++ F +      FSI+EATV DLQ+AF Q KLTSR+LVEFYI +I   N
Sbjct: 311 LFLSSLLLILTLSGFSAIVTSDGFSIKEATVHDLQVAFKQNKLTSRELVEFYIREIHNLN 370

Query: 68  PRLRGVMEVNPDALHLANKADRKRQEKAPGSVPLSHGIPVLVKDNIATKGKLNTIAGSFA 127
           P +RGV+EVNPDALH A+ ADR+R  +APGS    HGIP+L+KDNIATK KLNT AGSFA
Sbjct: 371 PLVRGVIEVNPDALHQADIADRERSARAPGSESGLHGIPILLKDNIATKDKLNTTAGSFA 430

Query: 128 LLGSIVPRDTGVVKKLRKVGAIILGKASLSEWSSFRSHKAPPDWNARMGQ---------- 187
           LLGS+VPRD GVV KLRK GAI+LGKASLSEW+ FRS+ AP  WNAR GQ          
Sbjct: 431 LLGSVVPRDAGVVTKLRKAGAIVLGKASLSEWAHFRSNDAPSGWNARAGQGKNPYVLSAD 490

Query: 188 ---------------------GTETDGSILCPSSFSSVVGIKRIIGLTSRAGVVPISPQQ 247
                                GTETDGSI+CPS+++SVVGIK  +GLTSRAGV+PISP+Q
Sbjct: 491 PCGSSSGSAISVAANMVAVSLGTETDGSIICPSNYNSVVGIKPTVGLTSRAGVIPISPRQ 550

Query: 248 DTVG-----------------------------FSYIPRGGYSPFLKADGLRGKRLGIVD 307
           DTVG                               +IP GGY  FLKADGL+GKRLGIV 
Sbjct: 551 DTVGPMCRTVSDAVYVLDAIVGFDRRDAATRKASKFIPNGGYKQFLKADGLKGKRLGIVR 610

Query: 308 GFFGGGPSISLAFEKILIILRKRGAILVNNLKIENLKTIISNHTASGEVTALRNEFKISL 367
             F    +    ++  L  LR+RGAILV++L+I N+  I SN   SGE TAL  EFK+SL
Sbjct: 611 NPF----TFPKEYQYHLNKLRQRGAILVDHLEIANIDVIFSN--TSGEDTALVAEFKLSL 670

Query: 368 NAYLKELVSSPIRSLSDSIAFNQKHADLEKIKEYGQELFRRSRDDERNRNGGSDI----- 413
           NAYLKEL++SP+RSL+D IAFN+K +DLE I ++GQ+LF     +  + NG  ++     
Sbjct: 671 NAYLKELITSPVRSLADIIAFNKKFSDLEMINKFGQKLFL----EAESTNGIGNVEKKAL 730

BLAST of Tan0020546 vs. NCBI nr
Match: KAG8368136.1 (hypothetical protein BUALT_Bualt15G0013700 [Buddleja alternifolia])

HSP 1 Score: 424.5 bits (1090), Expect = 1.1e-114
Identity = 252/477 (52.83%), Postives = 321/477 (67.30%), Query Frame = 0

Query: 1   MEAPSFSLSLSLFLILVAVAPFGSY-SVRIDFSIEEATVKDLQLAFNQKKLTSRQLVEFY 60
           M+A S S SL  FL LV +    SY ++    SI+EAT++D Q AF+Q +LTSRQLVEFY
Sbjct: 1   MKAQS-SFSLLSFLALVLLQWSHSYINICNSLSIKEATIQDFQTAFDQNQLTSRQLVEFY 60

Query: 61  IEQIRRYNPRLRGVMEVNPDALHLANKADRKRQEKAPGSVPLSHGIPVLVKDNIATKGKL 120
           + +I + NPRL+GV+EVNPDAL+LA+KADR+++ K P S+   HGIP+L+KDNIATK KL
Sbjct: 61  LNEIEKLNPRLKGVIEVNPDALYLADKADREKKAKLPCSLSRLHGIPILLKDNIATKDKL 120

Query: 121 NTIAGSFALLGSIVPRDTGVVKKLRKVGAIILGKASLSEWSSFRSHKAPPDWNARMGQ-- 180
           NT AGSFALLGS+ PRD GV  KLRK GAIILGKASLSEWS FRS +AP  W+AR G+  
Sbjct: 121 NTTAGSFALLGSVAPRDAGVAMKLRKAGAIILGKASLSEWSMFRSSRAPNGWSARGGKGK 180

Query: 181 -----------------------------GTETDGSILCPSSFSSVVGIKRIIGLTSRAG 240
                                        GTETDGSILCPS+F+SVVGIK  +GLTSRAG
Sbjct: 181 NPYVLSADPCGSSSGPAISVASNMVAVSLGTETDGSILCPSAFNSVVGIKPTVGLTSRAG 240

Query: 241 VVPISPQQDTVG------------------------------FSYIPRGGYSPFLKADGL 300
           V+PISP+QDT+G                                YIP+GGY+ FLKA+GL
Sbjct: 241 VIPISPRQDTIGPICRTVTDAVHVLDAIVGFDYNDARATRIASRYIPQGGYTQFLKANGL 300

Query: 301 RGKRLGIV-DGFF-GGGPSISLAFEKILIILRKRGAILVNNLKIENLKTIISNHTASGEV 360
           +GKRLGIV D FF      +   F++    LR+ GAILV+NL+I N+  I+   T SGEV
Sbjct: 301 KGKRLGIVRDPFFMFSNKIVKQTFKRHFQTLRQNGAILVDNLEIANINVILDG-TLSGEV 360

Query: 361 TALRNEFKISLNAYLKELVSSPIRSLSDSIAFNQKHADLEKIKEYGQELFRRSRDDERNR 407
           TA+  EFKISLNAYLKELV+SP+RSL+++IAFN+K++D+E I+EYGQE+F  S  +  N 
Sbjct: 361 TAMLAEFKISLNAYLKELVASPVRSLAEAIAFNKKNSDVEMIEEYGQEMFLAS--EATNG 420

BLAST of Tan0020546 vs. NCBI nr
Match: KAF3953674.1 (hypothetical protein CMV_020907 [Castanea mollissima])

HSP 1 Score: 424.1 bits (1089), Expect = 1.4e-114
Identity = 254/471 (53.93%), Postives = 311/471 (66.03%), Query Frame = 0

Query: 3   APSFSLSLSLFLILVAVAPFGSYSVRIDFSIEEATVKDLQLAFNQKKLTSRQLVEFYIEQ 62
           A SFS+  SL LIL+   P GS   +I+F I+E T+ DLQLAF Q KLTSRQLVEFY+++
Sbjct: 298 ALSFSIFSSLILILLLTIPSGSCH-QINFFIKEVTIHDLQLAFKQNKLTSRQLVEFYLKE 357

Query: 63  IRRYNPRLRGVMEVNPDALHLANKADRKRQEKAPGSVPLSHGIPVLVKDNIATKGKLNTI 122
           IRR NP L+GV+EVNPDAL  A+KAD +R+ K PGS    HGIP+L+KDNIATK KLNT 
Sbjct: 358 IRRLNPILKGVIEVNPDALSQADKADYERKAKVPGSQSRLHGIPILLKDNIATKDKLNTT 417

Query: 123 AGSFALLGSIVPRDTGVVKKLRKVGAIILGKASLSEWSSFRSHKAPPDWNARMGQ----- 182
           AGSFALLGS+VPRD GVV KLRK GAIILGKASL EWS FR+ + P  W+AR GQ     
Sbjct: 418 AGSFALLGSVVPRDAGVVSKLRKAGAIILGKASLGEWSHFRTARGPLAWSARGGQGKNPY 477

Query: 183 -------------------------GTETDGSILCPSSFSSVVGIKRIIGLTSRAGVVPI 242
                                    GTETDGSI+CP+SF+SVVGIK  +GLTSRAGV+PI
Sbjct: 478 TLGEPCGSSSGSSISVAANMVAVSLGTETDGSIICPASFNSVVGIKPTVGLTSRAGVIPI 537

Query: 243 SPQQDTVG-----------------------------FSYIPRGGYSPFLKADGLRGKRL 302
           SP+QDTVG                              SYIP GGY  FLK DGLRGKRL
Sbjct: 538 SPRQDTVGPICRTVSDAVHVLDAIAGIDNNDNATFKASSYIPSGGYGQFLKVDGLRGKRL 597

Query: 303 GIVDG---FFGGGPSISLAFEKILIILRKRGAILVNNLKIENLKTIISNHTASGEVTALR 362
           GIV      FG    ++  FE+    LR+RGAILV++L+I N+  I+ ++ ASGE TAL 
Sbjct: 598 GIVRNPFFDFGNNTYLNQTFEQHFDTLRQRGAILVDHLEITNIGMIL-DYKASGEETALL 657

Query: 363 NEFKISLNAYLKELVSSPIRSLSDSIAFNQKHADLEKIKEYGQELFRRSRDDER----NR 407
            EFKISLNAYLKELVSSP+R+++D IAFN K+ +LE I E  Q+ F  +   +      +
Sbjct: 658 AEFKISLNAYLKELVSSPVRTMADVIAFNNKNPELEMINEIDQDHFLSAEATKGIGKVEK 717

BLAST of Tan0020546 vs. NCBI nr
Match: RVW38569.1 (putative amidase C869.01 [Vitis vinifera])

HSP 1 Score: 423.7 bits (1088), Expect = 1.8e-114
Identity = 248/474 (52.32%), Postives = 314/474 (66.24%), Query Frame = 0

Query: 5   SFSLSLSLFLILVAVAPFGSYSVR---IDFSIEEATVKDLQLAFNQKKLTSRQLVEFYIE 64
           + S+     ++L+A+   GSY++R     F I+EA+V DL  AF Q KLTSRQLVEFY+ 
Sbjct: 18  AMSIHSHFLILLLALLSLGSYTIRGQGHGFYIKEASVDDLHTAFLQNKLTSRQLVEFYLG 77

Query: 65  QIRRYNPRLRGVMEVNPDALHLANKADRKRQEKAPGSVPLSHGIPVLVKDNIATKGKLNT 124
           +I R +P L GV+EVNPDAL+ A+KADR+R+ K+P S  + HGIP+L+KDNI TK KLNT
Sbjct: 78  EIFRLSPLLHGVIEVNPDALYQADKADRERKAKSPVSRSVLHGIPILLKDNIGTKDKLNT 137

Query: 125 IAGSFALLGSIVPRDTGVVKKLRKVGAIILGKASLSEWSSFRSHKAPPDWNARMGQ---- 184
            AGSFALL S+VPRD GVV KLRK GAIILGKASLSEW++FRS  APP W+AR GQ    
Sbjct: 138 TAGSFALLKSVVPRDAGVVMKLRKAGAIILGKASLSEWANFRSLNAPPGWSARGGQGLNP 197

Query: 185 ---------------------------GTETDGSILCPSSFSSVVGIKRIIGLTSRAGVV 244
                                      GTETDGSILCP+SF+SVVGIK  +GLTSRAGVV
Sbjct: 198 YVLSATPCGSSSGSAISVAANLVAVSLGTETDGSILCPASFNSVVGIKPTVGLTSRAGVV 257

Query: 245 PISPQQDTVG------------------------------FSYIPRGGYSPFLKADGLRG 304
           P+SP+QDT+G                                YIP GGY  FLK  GL+G
Sbjct: 258 PVSPRQDTIGPICRTVLDAVQVLDAIVGFDYNDAEATREASKYIPYGGYKQFLKPYGLKG 317

Query: 305 KRLGIV-DGFF--GGGPSISLAFEKILIILRKRGAILVNNLKIENLKTIISNHTASGEVT 364
           KRLGIV + FF  G G +++ AFE     LR+ GA+LV++L I N+  I+ N T+SGE T
Sbjct: 318 KRLGIVRNPFFTTGSGAALTQAFEHHFYTLRQEGAVLVDDLGIANIDVIL-NATSSGEAT 377

Query: 365 ALRNEFKISLNAYLKELVSSPIRSLSDSIAFNQKHADLEKIKEYGQELFRRSR-----DD 407
           AL  EFK+SLN+YLK+LV+SP+RSL+D IAFNQK +DLE IKE+GQ++F  ++      D
Sbjct: 378 ALLAEFKLSLNSYLKDLVASPVRSLADVIAFNQKFSDLEMIKEFGQDIFLAAQATNGIGD 437

BLAST of Tan0020546 vs. ExPASy TrEMBL
Match: A0A6J1CXI8 (putative amidase C869.01 OS=Momordica charantia OX=3673 GN=LOC111015494 PE=4 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 5.7e-146
Identity = 304/473 (64.27%), Postives = 346/473 (73.15%), Query Frame = 0

Query: 1   MEAPSFSLSLSLFLILVAVAPFGSYSVR-IDFSIEEATVKDLQLAFNQKKLTSRQLVEFY 60
           ME P  SLS SLFLILVAV+PF SYSVR   F IEEA +KDL LAF Q KLTSRQLVEFY
Sbjct: 1   METPRLSLSFSLFLILVAVSPFWSYSVRPAGFPIEEAGLKDLHLAFKQNKLTSRQLVEFY 60

Query: 61  IEQIRRYNPRLRGVMEVNPDALHLANKADRKRQEKAPGSVPLSHGIPVLVKDNIATKGKL 120
           I+QIRRYNPRLRGV+EVNPDAL+LA+KADR+R+ KAPGS+PL HGIPVLVKDN+ATK KL
Sbjct: 61  IKQIRRYNPRLRGVIEVNPDALYLADKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKL 120

Query: 121 NTIAGSFALLGSIVPRDTGVVKKLRKVGAIILGKASLSEWSSFRSHKAPPDWNARMGQ-- 180
           NT AGS ALLGS+VPRD G V +LRK GAIILGKAS+SEW+ FRS+ AP  WNAR GQ  
Sbjct: 121 NTTAGSLALLGSVVPRDAGAVMRLRKAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGR 180

Query: 181 ----------------------------GTETDGSILCPSSFSSVVGIKRIIGLTSRAGV 240
                                       GTETDGSILCPSSF+SVVGIK  +GLTS AGV
Sbjct: 181 EPYTLGLPCGSSSGSAISVSANMAAVALGTETDGSILCPSSFNSVVGIKPTLGLTSTAGV 240

Query: 241 VPISPQQDTVG-----------------------------FSYIPRGGYSPFLKADGLRG 300
           VPISP+QDTVG                               YIPRGGY  FLKADGLRG
Sbjct: 241 VPISPRQDTVGPICRTVSDAAYVLDVIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRG 300

Query: 301 KRLGIVDGFFG-GGPSISLAFEKILIILRKRGAILVNNLKIENLKTIISNHTASGEVTAL 360
           KRLGIV+ FFG   PS+  AFE+I  IL KRGAILV+NLKI+NL  I  N+T SGEV AL
Sbjct: 301 KRLGIVEDFFGVVDPSLIPAFEEIFTILSKRGAILVDNLKIDNLGAIY-NNTESGEVAAL 360

Query: 361 RNEFKISLNAYLKELVSSPIRSLSDSIAFNQKHADLEKIKEYGQELFRRSRDDERNRNGG 408
            NEFK+SLNAYLKELVSSPIRSL+++IAFNQKH+ LEKIKEYGQ+LF ++  +  N  G 
Sbjct: 361 LNEFKVSLNAYLKELVSSPIRSLAEAIAFNQKHSSLEKIKEYGQDLFLQA--EATNGMGI 420

BLAST of Tan0020546 vs. ExPASy TrEMBL
Match: A0A5B6YIK7 (Amidase domain-containing protein OS=Davidia involucrata OX=16924 GN=Din_000704 PE=4 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 2.3e-115
Identity = 250/476 (52.52%), Postives = 320/476 (67.23%), Query Frame = 0

Query: 6   FSLSLSLFLILVAVAPFGSYSVRID-FSIEEATVKDLQLAFNQKKLTSRQLVEFYIEQIR 65
           +SL+L   LILVA++  GS+ ++    SI+EAT+ D+Q +F Q +LTSRQLVEFY+EQI 
Sbjct: 5   YSLNL---LILVALSLLGSHIIKCHALSIKEATLHDIQTSFKQNQLTSRQLVEFYLEQIH 64

Query: 66  RYNPRLRGVMEVNPDALHLANKADRKRQEKAPGSVPLSHGIPVLVKDNIATKGKLNTIAG 125
           R NP LRGV+EVNPDAL+ A+KADR+R+ KAPGS+   HGIP+LVKDNIATK KLNT AG
Sbjct: 65  RLNPVLRGVIEVNPDALYEADKADRERKAKAPGSLFGLHGIPILVKDNIATKDKLNTTAG 124

Query: 126 SFALLGSIVPRDTGVVKKLRKVGAIILGKASLSEWSSFRSHKAPPDWNARMGQ------- 185
           SF+LLGS+VPRD GVV KLRK GAIILGKASLSEW++FRS  AP  W+AR G        
Sbjct: 125 SFSLLGSVVPRDAGVVMKLRKAGAIILGKASLSEWANFRSVAAPNGWSARGGHGKNPYVL 184

Query: 186 ------------------------GTETDGSILCPSSFSSVVGIKRIIGLTSRAGVVPIS 245
                                   GTETDGSILCP+SF++VVGIK  +GLTSRAGV+P+S
Sbjct: 185 SADPCGSSSGSAISVAANMVAVSLGTETDGSILCPASFNAVVGIKPTVGLTSRAGVIPVS 244

Query: 246 PQQDTVG------------------------------FSYIPRGGYSPFLKADGLRGKRL 305
           P+QDT+G                                YIPRGGY+ FLK DGLRGKRL
Sbjct: 245 PRQDTIGPICRTVSDAVYVLDAIAGFDHNDAEATREASKYIPRGGYAQFLKFDGLRGKRL 304

Query: 306 GIVDG---FFGGGPSISLAFEKILIILRKRGAILVNNLKIENLKTIISNHTASGEVTALR 365
           GI+      F     +   FE+    LR+RGAILV++L+I N+  I+ + T SGE TAL 
Sbjct: 305 GIIRKPFFIFANDSVLPHVFEQHFQTLRQRGAILVDHLEIVNIDMIL-DFTLSGEATALV 364

Query: 366 NEFKISLNAYLKELVSSPIRSLSDSIAFNQKHADLEKIKEYGQELFRRSRDDERNRNGGS 410
            EFK+SLNAYLK+LV+SP+RSL+D IAFNQK++DLE I+E+GQ +F  +  +  N  G +
Sbjct: 365 AEFKLSLNAYLKDLVASPVRSLADVIAFNQKNSDLEMIEEFGQAIFLAA--EATNGIGDA 424

BLAST of Tan0020546 vs. ExPASy TrEMBL
Match: A0A2N9I444 (Amidase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS46716 PE=4 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 3.0e-115
Identity = 253/470 (53.83%), Postives = 309/470 (65.74%), Query Frame = 0

Query: 11  SLFLILVAVAPFGSYSVR-IDFSIEEATVKDLQLAFNQKKLTSRQLVEFYIEQIRRYNPR 70
           SL LIL+A+   GS+ +    FSI EAT+ DLQLAF Q +LTSRQLVEFY+ +IRR NP 
Sbjct: 16  SLLLILLAILSSGSHIITGYGFSIREATIHDLQLAFKQNQLTSRQLVEFYLGEIRRLNPI 75

Query: 71  LRGVMEVNPDALHLANKADRKRQEKAPGSVPLSHGIPVLVKDNIATKGKLNTIAGSFALL 130
           L+GV+EVNPDAL+ A+KAD +R  K P S+   HGIP+LVKD I TK KLNT AGSFALL
Sbjct: 76  LKGVIEVNPDALYEADKADHERNAKVPKSLSGLHGIPILVKDTIGTKDKLNTTAGSFALL 135

Query: 131 GSIVPRDTGVVKKLRKVGAIILGKASLSEWSSFRSHKAPPDWNARMGQ------------ 190
           GS+VPRD  VV KLR  GAIILGKASLSEW+SFRS KAP  W+AR GQ            
Sbjct: 136 GSVVPRDASVVIKLRNAGAIILGKASLSEWASFRSLKAPDGWSARGGQGKNPYVLSADPC 195

Query: 191 -------------------GTETDGSILCPSSFSSVVGIKRIIGLTSRAGVVPISPQQDT 250
                              GTETDGSILCP+SF+SVVGIK  +GLTSRAGV+P+SP+QDT
Sbjct: 196 GSSSGSAISVASNLVAVALGTETDGSILCPASFNSVVGIKPTVGLTSRAGVIPVSPRQDT 255

Query: 251 VG-----------------------------FSYIPRGGYSPFLKADGLRGKRLGIV-DG 310
           +G                               YIPRGGY  FLKA GL+GKRLGIV + 
Sbjct: 256 IGPITRTVSDAVYVLDAIAGFDYYDEATRDASKYIPRGGYKQFLKAYGLKGKRLGIVRNP 315

Query: 311 FF--GGGPSISLAFEKILIILRKRGAILVNNLKIENLKTIISNHTASGEVTALRNEFKIS 370
           FF  G G   + AFE     LR+ GA+L+++L+I N+   + N T SGE TAL  EFK+S
Sbjct: 316 FFTSGTGSVQAKAFEYHFQTLRQGGAVLIDHLEIANIDVSL-NATFSGEGTALLAEFKLS 375

Query: 371 LNAYLKELVSSPIRSLSDSIAFNQKHADLEKIKEYGQELFRRSRDDERNRNGGSD----- 410
           LNAYLK+LVSSP+RSL+D IAFN+K +DLE IKE+GQ++F  ++    N  G  +     
Sbjct: 376 LNAYLKDLVSSPVRSLADVIAFNEKFSDLEMIKEFGQDIFLAAQ--ATNGIGNKEKAALL 435

BLAST of Tan0020546 vs. ExPASy TrEMBL
Match: A0A438DSV4 (Putative amidase C869.01 OS=Vitis vinifera OX=29760 GN=SPAC869.01_12 PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 8.8e-115
Identity = 248/474 (52.32%), Postives = 314/474 (66.24%), Query Frame = 0

Query: 5   SFSLSLSLFLILVAVAPFGSYSVR---IDFSIEEATVKDLQLAFNQKKLTSRQLVEFYIE 64
           + S+     ++L+A+   GSY++R     F I+EA+V DL  AF Q KLTSRQLVEFY+ 
Sbjct: 18  AMSIHSHFLILLLALLSLGSYTIRGQGHGFYIKEASVDDLHTAFLQNKLTSRQLVEFYLG 77

Query: 65  QIRRYNPRLRGVMEVNPDALHLANKADRKRQEKAPGSVPLSHGIPVLVKDNIATKGKLNT 124
           +I R +P L GV+EVNPDAL+ A+KADR+R+ K+P S  + HGIP+L+KDNI TK KLNT
Sbjct: 78  EIFRLSPLLHGVIEVNPDALYQADKADRERKAKSPVSRSVLHGIPILLKDNIGTKDKLNT 137

Query: 125 IAGSFALLGSIVPRDTGVVKKLRKVGAIILGKASLSEWSSFRSHKAPPDWNARMGQ---- 184
            AGSFALL S+VPRD GVV KLRK GAIILGKASLSEW++FRS  APP W+AR GQ    
Sbjct: 138 TAGSFALLKSVVPRDAGVVMKLRKAGAIILGKASLSEWANFRSLNAPPGWSARGGQGLNP 197

Query: 185 ---------------------------GTETDGSILCPSSFSSVVGIKRIIGLTSRAGVV 244
                                      GTETDGSILCP+SF+SVVGIK  +GLTSRAGVV
Sbjct: 198 YVLSATPCGSSSGSAISVAANLVAVSLGTETDGSILCPASFNSVVGIKPTVGLTSRAGVV 257

Query: 245 PISPQQDTVG------------------------------FSYIPRGGYSPFLKADGLRG 304
           P+SP+QDT+G                                YIP GGY  FLK  GL+G
Sbjct: 258 PVSPRQDTIGPICRTVLDAVQVLDAIVGFDYNDAEATREASKYIPYGGYKQFLKPYGLKG 317

Query: 305 KRLGIV-DGFF--GGGPSISLAFEKILIILRKRGAILVNNLKIENLKTIISNHTASGEVT 364
           KRLGIV + FF  G G +++ AFE     LR+ GA+LV++L I N+  I+ N T+SGE T
Sbjct: 318 KRLGIVRNPFFTTGSGAALTQAFEHHFYTLRQEGAVLVDDLGIANIDVIL-NATSSGEAT 377

Query: 365 ALRNEFKISLNAYLKELVSSPIRSLSDSIAFNQKHADLEKIKEYGQELFRRSR-----DD 407
           AL  EFK+SLN+YLK+LV+SP+RSL+D IAFNQK +DLE IKE+GQ++F  ++      D
Sbjct: 378 ALLAEFKLSLNSYLKDLVASPVRSLADVIAFNQKFSDLEMIKEFGQDIFLAAQATNGIGD 437

BLAST of Tan0020546 vs. ExPASy TrEMBL
Match: A5B8N1 (Amidase domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_016638 PE=4 SV=1)

HSP 1 Score: 423.3 bits (1087), Expect = 1.2e-114
Identity = 246/465 (52.90%), Postives = 311/465 (66.88%), Query Frame = 0

Query: 14  LILVAVAPFGSYSVR---IDFSIEEATVKDLQLAFNQKKLTSRQLVEFYIEQIRRYNPRL 73
           ++L+A+   GSY++R     F I+EA+V DL  AF Q KLTSRQLVEFY+ +I R +P L
Sbjct: 9   ILLLALLSLGSYTIRGQGHGFYIKEASVDDLHTAFLQNKLTSRQLVEFYLGEIFRLSPLL 68

Query: 74  RGVMEVNPDALHLANKADRKRQEKAPGSVPLSHGIPVLVKDNIATKGKLNTIAGSFALLG 133
            GV+EVNPDAL+ A+KADR+R+ K+P S  + HGIP+L+KDNI TK KLNT AGSFALL 
Sbjct: 69  HGVIEVNPDALYQADKADRERKAKSPVSRSVLHGIPILLKDNIGTKDKLNTTAGSFALLK 128

Query: 134 SIVPRDTGVVKKLRKVGAIILGKASLSEWSSFRSHKAPPDWNARMGQ------------- 193
           S+VPRD GVV KLRK GAIILGKASLSEW++FRS  APP W+AR GQ             
Sbjct: 129 SVVPRDAGVVMKLRKAGAIILGKASLSEWANFRSLNAPPGWSARGGQGLNPYVLSATPCG 188

Query: 194 ------------------GTETDGSILCPSSFSSVVGIKRIIGLTSRAGVVPISPQQDTV 253
                             GTETDGSILCP+SF+SVVGIK  +GLTSRAGVVP+SP+QDT+
Sbjct: 189 SSSGSAISVAANLVAVSLGTETDGSILCPASFNSVVGIKPTVGLTSRAGVVPVSPRQDTI 248

Query: 254 G------------------------------FSYIPRGGYSPFLKADGLRGKRLGIV-DG 313
           G                                YIP GGY  FLK  GL+GKRLGIV + 
Sbjct: 249 GPICRTVLDAVQVLDAIVGFDYNDAEATREASKYIPYGGYKQFLKPYGLKGKRLGIVRNP 308

Query: 314 FF--GGGPSISLAFEKILIILRKRGAILVNNLKIENLKTIISNHTASGEVTALRNEFKIS 373
           FF  G G +++ AFE     LR+ GA+LV++L+I N+  I+ N T+SGE TAL  EFK+S
Sbjct: 309 FFTTGSGAALTQAFEHHFYTLRQEGAVLVDDLEIANINVIL-NATSSGEATALLAEFKLS 368

Query: 374 LNAYLKELVSSPIRSLSDSIAFNQKHADLEKIKEYGQELFRRSR-----DDERNRNGGSD 407
           LN+YLK+LV+SP+RSL+D IAFNQK +DLE IKE+GQ++F  ++      D   +   + 
Sbjct: 369 LNSYLKDLVASPVRSLADVIAFNQKFSDLEMIKEFGQDIFLAAQATNGIGDVEKKALSNL 428

BLAST of Tan0020546 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 351.7 bits (901), Expect = 8.2e-97
Identity = 215/437 (49.20%), Postives = 278/437 (63.62%), Query Frame = 0

Query: 7   SLSLSLFLILVAVAPFGSYS-VRID--FSIEEATVKDLQLAFNQKKLTSRQLVEFYIEQI 66
           SL + L L    +   GS S +R+   FSI+EAT++D+++AFN+K+LTS+QLVE Y+E I
Sbjct: 11  SLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAI 70

Query: 67  RRYNPRLRGVMEVNPDALHLANKADRKRQEKAPGSVPLSHGIPVLVKDNIATKGKLNTIA 126
            + NP L  V+E NPDAL  A  ADR+R  K    +P+ HG+PVL+KD+I+TK KLNT A
Sbjct: 71  SKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNTTA 130

Query: 127 GSFALLGSIVPRDTGVVKKLRKVGAIILGKASLSEWSSFRSHKAPPDWNARMGQGTETDG 186
           GSFALLGS+V RD GVVK+LR+ GA+ILGKASLSEW+ FRS   P  W+A          
Sbjct: 131 GSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA---------- 190

Query: 187 SILCPSSFSSVVGIKRIIGLTSRAGVVPISPQQDTVG----------------------- 246
                 S +SVVGIK  +GLTSRAGVVPIS +QD++G                       
Sbjct: 191 -----PSQNSVVGIKPSVGLTSRAGVVPISLRQDSIGPICRTVSDAVHLLDAIVGYDPLD 250

Query: 247 ------FSYIPRGGYSPFLKADGLRGKRLGIVDGFFGGGPSISLAFEKILIILRKRGAIL 306
                   +IP GGY  FL   GL+GKRLGIV          S   +  +  LR+ GAI+
Sbjct: 251 EATKTASEFIPEGGYKQFLTTSGLKGKRLGIV-------MKHSSLLDHHIKTLRREGAIV 310

Query: 307 VNNLKIENLKTIISNHTASGEVTALRNEFKISLNAYLKELVSSPIRSLSDSIAFNQKHAD 366
           +NNL I N++ I+   T SGE  AL  EFK+SLNAYLKELV SP+RSL+D IA+N++ A+
Sbjct: 311 INNLTIPNIEVIVGG-TDSGEEIALLAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAE 370

Query: 367 LEKIKEYGQELF----RRSRDDERNRNGGSDIK-ISEDGIEKTITENKLDAIVTSSARIS 407
            EK+KE+GQE+F      S   E+ +     +K +S +GIEK I ENKLDAIVT  + +S
Sbjct: 371 QEKVKEWGQEVFLTAEATSGMGEKEKTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLS 424

BLAST of Tan0020546 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 71.6 bits (174), Expect = 1.6e-12
Identity = 57/196 (29.08%), Postives = 97/196 (49.49%), Query Frame = 0

Query: 51  TSRQLVEFYIEQIRRYNPRLRGVMEVNPDALHLANKADRKRQEKAPGSVPLSHGIPVLVK 110
           T+ ++ + Y+ +IR   P+L+  + V+ + L  A + D +R  K     PL+ G+ + VK
Sbjct: 59  TAVEIAKSYLSRIRLTEPQLKCFLHVSENVLKDAQEID-QRIAKGEELGPLA-GVLIGVK 118

Query: 111 DNIATKGKLNTIAGSFALLGSIVPRDTGVVKKLRKVGAIILGKASLSEW--------SSF 170
           DNI T+G  +T A S  L     P D   VKK++++G I++GK ++ E+        S+F
Sbjct: 119 DNICTQGMPST-AASRILEHYRPPFDATAVKKIKELGGIVVGKTNMDEFGMGSTTEASAF 178

Query: 171 RSHKAPPDWN------------------ARMGQGTETDGSILCPSSFSSVVGIKRIIGLT 221
           +    P D +                    +  G++T GS+  P+SF  VVG+K   G  
Sbjct: 179 QVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKPTYGRV 238

BLAST of Tan0020546 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 64.3 bits (155), Expect = 2.6e-10
Identity = 51/200 (25.50%), Postives = 89/200 (44.50%), Query Frame = 0

Query: 37  TVKDLQLAFNQKKLTSRQLVEFYIEQIRRYNPRLRGVMEVNPDALHLANKADRKRQEKAP 96
           +V +L      +++TS++LV  Y++Q++RYN  L  V+    +  +   K       +  
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGT 263

Query: 97  GSVPLSHGIPVLVKDNIATKGKLNTIAGSFALLGSIVPRDTGVVKKLRKVGAIILGK--- 156
              PL HGIP  +KD +A  G   T  GS +     +  +  V K+L+  GA+++ K   
Sbjct: 264 YLGPL-HGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVT 323

Query: 157 ASLSEWSSFRSHKAPPDWN-------------ARMGQGTETDGSILCPSSFSSVVGIKRI 216
            S++    +   +    WN             A    G+ET GS+  P++   +  ++  
Sbjct: 324 GSMAYDDIWFGGRTRNPWNIEEFSTGSSAGPAASTSAGSETAGSMTYPAARCGITALRPT 383

Query: 217 IGLTSRAGVVPISPQQDTVG 221
            G   R GV+ IS   D +G
Sbjct: 384 FGSVGRTGVMSISESLDKLG 401

BLAST of Tan0020546 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 61.2 bits (147), Expect = 2.2e-09
Identity = 52/207 (25.12%), Postives = 91/207 (43.96%), Query Frame = 0

Query: 37  TVKDLQLAFNQKKLTSRQLVEFYIEQIRRYNPRLRGVMEVNPDALHLANKADRKRQEKAP 96
           +V +L      +++TS++LV  Y++Q++RYN  L  V+    +  +   K       +  
Sbjct: 204 SVLELGELIKTRQITSKELVRIYLKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGT 263

Query: 97  GSVPLSHGIPVLVKDNIATKGKLNTIAGSFALLGSIVPRDTGVVKKLRKVGAIILGKA-- 156
              PL HGIP  +KD +A  G   T  GS +     +  +  V K+L+  GA+++ K   
Sbjct: 264 YLGPL-HGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVT 323

Query: 157 ------------------SLSEWSSFRSHKAPPDWNARM---GQGTETDGSILCPSSFSS 216
                             ++ E+S+  S       +A M     G+ET GS+  P++   
Sbjct: 324 GSMAYDDIWFGGRTRNPWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSMTYPAARCG 383

Query: 217 VVGIKRIIGLTSRAGVVPISPQQDTVG 221
           +  ++   G   R GV+ IS   D +G
Sbjct: 384 ITALRPTFGSVGRTGVMSISESLDKLG 408

BLAST of Tan0020546 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 45.8 bits (107), Expect = 9.6e-05
Identity = 47/200 (23.50%), Postives = 81/200 (40.50%), Query Frame = 0

Query: 38  VKDLQLAFNQKKLTSRQLVEFYIEQIRRY---NPRLRGVMEVNPDALHLANKADRKRQEK 97
           ++D   A+  K  T  Q+ +  I  I  +    P    ++  + + +    +A  +R E+
Sbjct: 131 IRDYAYAYRSKLTTPLQVAKRIISIIEEFGYDKPPTPFLIRFDANEVIKQAEASTRRFEQ 190

Query: 98  APGSVPLSHGIPVLVKDNIATKGKLNTIAGSFALLGSIVPRDTGVVKKLRKVGAIILGKA 157
               + +  GI V +KD+I           ++      V +D+ VV KLR  GAI+LGKA
Sbjct: 191 G-NPISVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDSAVVSKLRSCGAILLGKA 250

Query: 158 SLSEW--------SSFRSHKAPPDWNARMG------------------QGTETDGSILCP 209
           ++ E         S++ + + P D     G                   GT+  GS+  P
Sbjct: 251 NMHELGMGTTGNNSNYGTTRNPHDPKRYTGGSSSGSAAIVAAGLCSAALGTDGGGSVRIP 310