Tan0020457 (gene) Snake gourd v1

BLAST of Tan0020457 vs. ExPASy Swiss-Prot
Match: H6U1I8 (GDSL lipase OS=Tanacetum cinerariifolium OX=118510 GN=GLIP PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 5.5e-84
Identity = 158/336 (47.02%), Postives = 217/336 (64.58%), Query Frame = 0

Query: 41  SKKRFGFFIFGDSYVDVGNNNYINTTSDFQANFPPYGESFFHIPTGRFTDGRTITDFLGE 100
           S++    FIFGDS  D GNNN+INT  +F+ANF PYG+S+F  PTGRF+DGR I DF+ E
Sbjct: 28  SQQAAALFIFGDSVFDPGNNNHINTHVNFKANFWPYGQSYFSSPTGRFSDGRIIPDFIAE 87

Query: 101 YAGLPLIPPYLDPHNNFYEIGVNFASGGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKK 160
           YA LP+IP YL+P+N+F   G NFAS GAGAL  SH   A+GLQTQ+++F  +V   R+ 
Sbjct: 88  YASLPIIPAYLEPNNDFTH-GANFASAGAGALIASHAGLAVGLQTQLRYFGDLVDHYRQN 147

Query: 161 LGNARAQSFLSNSVFFFNFGGNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVY 220
           LG+ +++  LS++V+ F+ GGNDY +P+   Y        QEQ+V++VIGNM+  +K +Y
Sbjct: 148 LGDIKSRQLLSDAVYLFSCGGNDYQSPY---YPY-----TQEQYVDIVIGNMTNVIKGIY 207

Query: 221 KYGGRKFGFMTVPPLGYMPNSRMKKSVQFFE-EASSIARLHNKLLPDAVHKLAIQLKGFK 280
           + GGRKFG + VP +G  P  R K+       E   + RLHN+     + +L  QL+GF 
Sbjct: 208 EKGGRKFGVVNVPLIGCWPGMRAKQPGNTCNTEVDELTRLHNQAFAKRLEQLEKQLEGFV 267

Query: 281 YAFADTHTLLLQRILNPTEYGLKIVETACCGSEEFKGFYNCGRKRGSLPFTHCDNLEDYM 340
           YA  D  T +L R+ NP++YG K  E+ACCGS  F G Y+CGR +    F  CDN  +Y 
Sbjct: 268 YAKFDLSTAILNRMKNPSKYGFKEGESACCGSGPFGGNYDCGRIK---EFGLCDNATEYF 327

Query: 341 FYDSFHPTEKVFKQIAQLMWSGDAEIVKPCNFKQLF 376
           F+D FHP E   +Q A++ W GD+ + +P N K LF
Sbjct: 328 FFDPFHPNELASRQFAEMFWDGDSMVTQPYNLKALF 351

BLAST of Tan0020457 vs. ExPASy Swiss-Prot
Match: Q9SYF5 (GDSL esterase/lipase 3 OS=Arabidopsis thaliana OX=3702 GN=GLIP3 PE=2 SV=2)

HSP 1 Score: 295.8 bits (756), Expect = 7.0e-79
Identity = 154/334 (46.11%), Postives = 209/334 (62.57%), Query Frame = 0

Query: 48  FIFGDSYVDVGNNNYINTTSDFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLI 107
           F+FGDS  D GNNNYINT S F++N  PYG++ F  PTGR +DG        E A LP I
Sbjct: 37  FVFGDSLFDAGNNNYINTVSSFRSNIWPYGQTNFKFPTGRLSDG-------PEKAWLPSI 96

Query: 108 PPYLDPH--NNFYEIGVNFASGGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNAR 167
           PP L P+  NN +  GV+FAS GAGALA+S     I L TQ+  FK V KSLR +LG+A 
Sbjct: 97  PPNLQPNNGNNQFTYGVSFASAGAGALAESFLGMVINLGTQLNNFKDVEKSLRSELGDAE 156

Query: 168 AQSFLSNSVFFFNFGGNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGR 227
            +   S +V+ F+ G NDY  PF  +   F + N++E+FV+ VIGN++  ++EVYK GGR
Sbjct: 157 TKRVFSRAVYLFHIGANDYFYPFSANSSTFKS-NSKEKFVDFVIGNITFVIEEVYKMGGR 216

Query: 228 KFGFMTVPPLGYMPNSRMKKSVQF---FEEASSIARLHNKLLPDAVHKLAIQLKGFKYAF 287
           KFGF+ V P    PNS ++   +    F+  + +  +HNK  PD + +L  QL GF+YA 
Sbjct: 217 KFGFLNVGPYECSPNSLIRDRTKIGSCFKPVAELIDMHNKKFPDVLRRLQRQLSGFRYAL 276

Query: 288 ADTHTLLLQRILNPTEYGLKIVETACCGSEEFKGFYNCGRKRG-SLPFTHCDNLEDYMFY 347
            D HT L +RI +P++YG K  + ACCGS   +G   CG + G S  +  C+N+ DY+FY
Sbjct: 277 HDYHTSLSERINSPSKYGFKEGKKACCGSGPLRGINTCGNRIGPSQGYGLCENVTDYLFY 336

Query: 348 DSFHPTEKVFKQIAQLMWSGDAEIVKPCNFKQLF 376
           DS H TEK  +QIA+L+W+G   + +P N K LF
Sbjct: 337 DSSHLTEKAHRQIAELIWNGPPNVTRPYNLKALF 362

BLAST of Tan0020457 vs. ExPASy Swiss-Prot
Match: Q9FLN0 (GDSL esterase/lipase 1 OS=Arabidopsis thaliana OX=3702 GN=GLIP1 PE=1 SV=1)

HSP 1 Score: 294.7 bits (753), Expect = 1.6e-78
Identity = 152/337 (45.10%), Postives = 210/337 (62.31%), Query Frame = 0

Query: 48  FIFGDSYVDVGNNNYINTTSDFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLI 107
           F+FGDS  D GNNNYI+T S  ++N+ PYG++ F  PTGR +DGR I DF+ EYA LPLI
Sbjct: 39  FVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAWLPLI 98

Query: 108 PPYLDPH--NNFYEIGVNFASGGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNAR 167
           PP L P   N+ +  GVNFASGGAGAL  +     I L+TQ+  FKKV + LR KLG+A 
Sbjct: 99  PPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLVINLRTQLNNFKKVEEMLRSKLGDAE 158

Query: 168 AQSFLSNSVFFFNFGGNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGR 227
            +  +S +V+ F+ G NDY  PF  +  +F ++ + E++V+ V+GNM+   KEVY  GGR
Sbjct: 159 GKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSI-SNEKYVDYVVGNMTDVFKEVYNLGGR 218

Query: 228 KFGFMTVPPLGYMPNSRM---KKSVQFFEEASSIARLHNKLLPDAVHKLAIQLKGFKYAF 287
           KFG +   P    P S +    K    F+  + +  +HN+ L + + +L  +L GFKYA 
Sbjct: 219 KFGILNTGPYDCAPASLVIDQTKIRSCFQPVTELINMHNEKLLNGLRRLNHELSGFKYAL 278

Query: 288 ADTHTLLLQRILNPTEYGLKIVETACCGSEEFKGFYNCGRKRG-SLPFTHCDNLEDYMFY 347
            D HT L +R+ +P++YG K  + ACCGS   +G   CG + G S  +  C+N+ DY+F+
Sbjct: 279 HDYHTSLSERMNDPSKYGFKEGKKACCGSGPLRGINTCGGRMGLSQSYELCENVTDYLFF 338

Query: 348 DSFHPTEKVFKQIAQLMWSGDAEIVKPCNFKQLFFQN 379
           D FH TEK  +QIA+L+WSG   I  P N K LF  N
Sbjct: 339 DPFHLTEKANRQIAELIWSGPTNITGPYNLKALFELN 374

BLAST of Tan0020457 vs. ExPASy Swiss-Prot
Match: Q9SSA7 (GDSL esterase/lipase 5 OS=Arabidopsis thaliana OX=3702 GN=GLIP5 PE=2 SV=2)

HSP 1 Score: 294.7 bits (753), Expect = 1.6e-78
Identity = 155/376 (41.22%), Postives = 234/376 (62.23%), Query Frame = 0

Query: 10  FVFLFFSN--LFAAQSAFPTITEASRNQVKLPWSKKRFGFFIFGDSYVDVGNNNYINTTS 69
           F+F   S+  LF A  +   I+    N V           F+FGDS++D GNNNYINTT+
Sbjct: 18  FIFFIVSSTILFLAGKSSAKISHNGDNNVT--------ALFLFGDSFLDAGNNNYINTTT 77

Query: 70  DFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLIPPYLDPHNNFYEI-GVNFAS 129
             QANFPPYG++FF +PTGRF+DGR I+DF+ EYA LPLIPP+L+P N+  ++ GVNFAS
Sbjct: 78  LDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNFAS 137

Query: 130 GGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNARAQSFLSNSVFFFNFGGNDYLN 189
            GAGAL ++   S I L+TQ+  +KKV +  R   G   ++  +S +V+  + G NDY +
Sbjct: 138 AGAGALVETFQGSVINLRTQLDHYKKVERLWRTNFGKEESKKRISRAVYLISIGSNDYSS 197

Query: 190 PFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGRKFGFMTVPPLGYMPNSRM--- 249
            F  +  +  +++   Q V++VIGN++  + E+YK GGRKFGF+ VP LG  P  R+   
Sbjct: 198 IFLTNQSLPISMS---QHVDIVIGNLTTFIHEIYKIGGRKFGFLNVPDLGCFPALRILQP 257

Query: 250 KKSVQFFEEASSIARLHNKLLPDAVHKLAIQLKGFKYAFADTHTLLLQRILNPTEYGLKI 309
           K       +AS +A +HN+ L + + ++  Q+KGFK++  D +  L  R+ +P+++G K 
Sbjct: 258 KNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGFKFSLFDMNKSLRLRMQHPSKFGFKE 317

Query: 310 VETACCGSEEFKGFYNCGRKRGSLPFTHCDNLEDYMFYDSFHPTEKVFKQIAQLMWSG-- 369
            E ACCG+ +++G ++CG KR    +  C+N +DY+F+DS H T+  + Q A L+W+G  
Sbjct: 318 GEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSLHLTQNTYNQFANLIWNGGH 377

Query: 370 --DAEIVKPCNFKQLF 376
             D+ +V P N   LF
Sbjct: 378 MSDSLVVGPYNINNLF 382

BLAST of Tan0020457 vs. ExPASy Swiss-Prot
Match: Q9SYF0 (GDSL esterase/lipase 2 OS=Arabidopsis thaliana OX=3702 GN=GLIP2 PE=2 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.0e-78
Identity = 151/337 (44.81%), Postives = 208/337 (61.72%), Query Frame = 0

Query: 48  FIFGDSYVDVGNNNYINTTSDFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLI 107
           F+FGDS  D GNNNYI+T   F++N+ PYG++ F  PTGR +DGRTI DF+ EYA LPLI
Sbjct: 41  FVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLI 100

Query: 108 PPYLDPHN--NFYEIGVNFASGGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNAR 167
           P YL P N  N +  GV+FAS GAGAL  +     I L++Q+  FKKV K LR  LG A+
Sbjct: 101 PAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGEAQ 160

Query: 168 AQSFLSNSVFFFNFGGNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGR 227
            +  +S +V+ F+ G NDY  PF  +  IF + + QE +V+ V+GN +  +KEVYK GGR
Sbjct: 161 GKMVISRAVYLFHIGVNDYQYPFSTNSSIFQS-SPQEIYVDFVVGNTTAVIKEVYKIGGR 220

Query: 228 KFGFMTVPPLGYMPNSRMKKSVQF---FEEASSIARLHNKLLPDAVHKLAIQLKGFKYAF 287
           KFGF+ +      P S +    +    F+  + +  LHN+ L   + +L  +L GFKYA 
Sbjct: 221 KFGFLNMGAYDCAPASLIIDQTKIGTCFKPVTELINLHNEKLESGLRRLERELSGFKYAL 280

Query: 288 ADTHTLLLQRILNPTEYGLKIVETACCGSEEFKGFYNCGRKRG-SLPFTHCDNLEDYMFY 347
            D HT L  R+ NP++YG K  + ACCG+   +G   CG + G S  +  C+ + DY+F+
Sbjct: 281 HDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVTDYLFF 340

Query: 348 DSFHPTEKVFKQIAQLMWSGDAEIVKPCNFKQLFFQN 379
           D FH TEK  +QIA+L+WSG   + KP N + LF  N
Sbjct: 341 DHFHLTEKAHQQIAELIWSGPTNVTKPYNLQALFELN 376

BLAST of Tan0020457 vs. NCBI nr
Match: XP_022928027.1 (GDSL esterase/lipase 3-like [Cucurbita moschata])

HSP 1 Score: 618.2 bits (1593), Expect = 4.7e-173
Identity = 301/385 (78.18%), Postives = 330/385 (85.71%), Query Frame = 0

Query: 1   MKFLNLGICFVFLFFSNLFAAQSAFPTITEASRNQVK-LPWSKKRFGFFIFGDSYVDVGN 60
           MKF NLG CF F+    LFAAQ  FPTI+EA   Q+   P S+ RFGFFIFGDSYVDVGN
Sbjct: 1   MKFPNLGSCFFFI----LFAAQCGFPTISEAVGIQLPGAPSSENRFGFFIFGDSYVDVGN 60

Query: 61  NNYINTTSDFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLIPPYLDPHNNFYE 120
           NNYINTTSDFQANFPPYGESFF + TGRFTDGR I DFLGEYA LPL+PPYLDPHN+ Y+
Sbjct: 61  NNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYD 120

Query: 121 IGVNFASGGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNARAQSFLSNSVFFFNF 180
            GVNFASGG GALA SH E A+GLQTQMKFFK+V KSLRKKLGNAR QSF SNSVF FNF
Sbjct: 121 YGVNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNF 180

Query: 181 GGNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGRKFGFMTVPPLGYMP 240
           GGNDYLNPFDISYDIF T++AQEQFVNMVIGN++ ALKEVYK+GGRKFG M VPPLGYMP
Sbjct: 181 GGNDYLNPFDISYDIFKTIDAQEQFVNMVIGNITKALKEVYKHGGRKFGLMGVPPLGYMP 240

Query: 241 NSRMKKSVQFFEEASSIARLHNKLLPDAVHKLAIQLKGFKYAFADTHTLLLQRILNPTEY 300
           +SR+KKS QFFEEASSIARLHNKLLP A+HKL+ QLKGFKYAFADTHTLLLQRILNP+ Y
Sbjct: 241 SSRLKKSAQFFEEASSIARLHNKLLPIALHKLSKQLKGFKYAFADTHTLLLQRILNPSRY 300

Query: 301 GLKIVETACCGSEEFKGFYNCGRKRGSLPFTHCDNLEDYMFYDSFHPTEKVFKQIAQLMW 360
           G K+V+TACCGS+EF+G YNCGRK GSLPFTHC NLED+MF+DSFHPTEKVFKQ+ + MW
Sbjct: 301 GFKVVDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLTEQMW 360

Query: 361 SGDAEIVKPCNFKQLFFQNDPMLAS 385
           SG  E+VKP NFKQLF      LAS
Sbjct: 361 SGGLEVVKPYNFKQLFDYEPTTLAS 381

BLAST of Tan0020457 vs. NCBI nr
Match: XP_023532785.1 (GDSL esterase/lipase 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 617.5 bits (1591), Expect = 8.0e-173
Identity = 298/385 (77.40%), Postives = 335/385 (87.01%), Query Frame = 0

Query: 1   MKFLNLGICFVFLFFSNLFAAQSAFPTITEASRNQVKLPWSKKRFGFFIFGDSYVDVGNN 60
           MK  NLG C  F FF  LFA Q+A PT  EAS   + LP     F FF+FGDSYVD GNN
Sbjct: 1   MKLPNLGFCCFFFFFFILFAFQTASPTFPEAS--TIPLP---NCFAFFLFGDSYVDAGNN 60

Query: 61  NYINTTSDFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLIPPYLDPHNNFYEI 120
           NYINTT+DFQANFPPYGE+FFH+PTGRFTDGRTI DFL EYAGLPLIPPYLDPHN+FY+ 
Sbjct: 61  NYINTTADFQANFPPYGETFFHLPTGRFTDGRTIPDFLAEYAGLPLIPPYLDPHNDFYDN 120

Query: 121 GVNFASGGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNARAQSFLSNSVFFFNFG 180
           GVNFAS G+GALA+SH  SAIGLQTQMKFFK V+KSLRKKLGNA AQ+ +SNSVF FNFG
Sbjct: 121 GVNFASSGSGALAESHEGSAIGLQTQMKFFKNVMKSLRKKLGNASAQTLISNSVFLFNFG 180

Query: 181 GNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGRKFGFMTVPPLGYMPN 240
           GNDYLNPFDISYDIFNT +AQEQFVNMV+GN++IALKEVYKYGGRKFGFMTVPPLG+MP+
Sbjct: 181 GNDYLNPFDISYDIFNTSSAQEQFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPS 240

Query: 241 SRMKKSVQFFEEASSIARLHNKLLPDAVHKLAIQLKGFKYAFADTHTLLLQRILNPTEYG 300
           SR+K+SVQFFEEASSI RLHN++LPDA+ KL IQL+GFKYAFADTHTLLLQRILNP +YG
Sbjct: 241 SRIKRSVQFFEEASSITRLHNQVLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYG 300

Query: 301 LKIVETACCGSEEFKGFYNCGRKRGSLPFTHCDNLEDYMFYDSFHPTEKVFKQIAQLMWS 360
            K+ ETACCGS  F+GFYNCGR    LPFTHCDNLEDY+F+DSFHPTEK+FKQ+A+L+WS
Sbjct: 301 FKVAETACCGSGAFRGFYNCGRNVEGLPFTHCDNLEDYIFFDSFHPTEKLFKQLAELIWS 360

Query: 361 GDAEIVKPCNFKQLFFQNDPMLASH 386
           GDA+IVKP NFKQL F+ D MLAS+
Sbjct: 361 GDAQIVKPYNFKQL-FEYDSMLASY 379

BLAST of Tan0020457 vs. NCBI nr
Match: XP_023512752.1 (GDSL esterase/lipase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 611.3 bits (1575), Expect = 5.7e-171
Identity = 298/385 (77.40%), Postives = 332/385 (86.23%), Query Frame = 0

Query: 1   MKFLNLGICFVFLFFSNLFAAQSAFPTITEASRNQV-KLPWSKKRFGFFIFGDSYVDVGN 60
           M F NLG CF F+    LFAA   FPTI++A   Q+   P S+ RFGFFIFGDSYVDVGN
Sbjct: 1   MMFPNLGSCFFFI----LFAALCGFPTISKAVGIQLGGAPSSENRFGFFIFGDSYVDVGN 60

Query: 61  NNYINTTSDFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLIPPYLDPHNNFYE 120
           NNYINTTSDFQANFPPYGESFF + TGRFTDGR I DFLGEYA LPL+PPYLDPHN+ Y+
Sbjct: 61  NNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYD 120

Query: 121 IGVNFASGGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNARAQSFLSNSVFFFNF 180
            GVNFASGG GALA SH E A+GLQTQMKFFK+V KSLRKKLGNAR QSF SNSVF FNF
Sbjct: 121 YGVNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNF 180

Query: 181 GGNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGRKFGFMTVPPLGYMP 240
           GGNDYLNPFDISYDIF T++AQEQ+VNMVIGN+++ALKEVYK+GGRKFG M VPPLGYMP
Sbjct: 181 GGNDYLNPFDISYDIFKTIDAQEQYVNMVIGNITMALKEVYKHGGRKFGLMGVPPLGYMP 240

Query: 241 NSRMKKSVQFFEEASSIARLHNKLLPDAVHKLAIQLKGFKYAFADTHTLLLQRILNPTEY 300
           +SR+KKS QFFEEASSIARLHNKLLP A+HKL+ QLK FKYAFADTHTLLLQRILNP +Y
Sbjct: 241 SSRLKKSAQFFEEASSIARLHNKLLPIALHKLSKQLKEFKYAFADTHTLLLQRILNPLQY 300

Query: 301 GLKIVETACCGSEEFKGFYNCGRKRGSLPFTHCDNLEDYMFYDSFHPTEKVFKQIAQLMW 360
           G K+V+TACCGS+EF+G YNCGRK GSLPFTHC NLED+MF+DSFHPTEKVFKQ+A+ MW
Sbjct: 301 GFKVVDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLAEQMW 360

Query: 361 SGDAEIVKPCNFKQLFFQNDPMLAS 385
           SG  E+VKP NFKQL F+ +P LAS
Sbjct: 361 SGGLEVVKPYNFKQL-FEYEPTLAS 380

BLAST of Tan0020457 vs. NCBI nr
Match: XP_022947487.1 (GDSL esterase/lipase 2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 609.4 bits (1570), Expect = 2.2e-170
Identity = 295/385 (76.62%), Postives = 333/385 (86.49%), Query Frame = 0

Query: 1   MKFLNLGICFVFLFFSNLFAAQSAFPTITEASRNQVKLPWSKKRFGFFIFGDSYVDVGNN 60
           MK  NLG CF F F   LFA  +A PT  EA+   + LP     F FF+FGDSYVD GNN
Sbjct: 1   MKLPNLGFCFFFFFI--LFALHTASPTFPEAT--TIPLP---NCFAFFLFGDSYVDAGNN 60

Query: 61  NYINTTSDFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLIPPYLDPHNNFYEI 120
           NYINTT+DFQANFPPYGE+FFH+PT RFTDGRTI DFL EYAGLPLIPPYLDPH NFY+ 
Sbjct: 61  NYINTTADFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDN 120

Query: 121 GVNFASGGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNARAQSFLSNSVFFFNFG 180
           GVNFAS G+GALA+SH  SAIGLQTQMKFFKKV+KSLRKKLGNA AQ+ +SNS+F FNFG
Sbjct: 121 GVNFASSGSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFG 180

Query: 181 GNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGRKFGFMTVPPLGYMPN 240
           GNDYLNPFDISYDIFNT +AQE+FVNMV+GN++IALKEVYKYGGRKFGFMTVPPLG+MP+
Sbjct: 181 GNDYLNPFDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPS 240

Query: 241 SRMKKSVQFFEEASSIARLHNKLLPDAVHKLAIQLKGFKYAFADTHTLLLQRILNPTEYG 300
           SR+K+SVQFFEEASSI RLHN+LLPDA+ KL IQL+GFKYAFADTHTLLLQRILNP +YG
Sbjct: 241 SRIKRSVQFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYG 300

Query: 301 LKIVETACCGSEEFKGFYNCGRKRGSLPFTHCDNLEDYMFYDSFHPTEKVFKQIAQLMWS 360
            K+VETACCGS  F+G YNCGR    LPFTHCDNLEDY+F+DSFHPTEK+FKQ+A+L+WS
Sbjct: 301 FKVVETACCGSGAFRGIYNCGRNVEGLPFTHCDNLEDYVFFDSFHPTEKLFKQLAELIWS 360

Query: 361 GDAEIVKPCNFKQLFFQNDPMLASH 386
           GDA+IVKP NFKQL F+ D MLAS+
Sbjct: 361 GDAQIVKPYNFKQL-FEYDSMLASY 377

BLAST of Tan0020457 vs. NCBI nr
Match: XP_023006840.1 (GDSL esterase/lipase 2-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 609.0 bits (1569), Expect = 2.8e-170
Identity = 295/385 (76.62%), Postives = 333/385 (86.49%), Query Frame = 0

Query: 1   MKFLNLGICFVFLFFSNLFAAQSAFPTITEASRNQVKLPWSKKRFGFFIFGDSYVDVGNN 60
           MK  NLG CF F F   LFA Q+A PT  EAS   + LP     FGFF+FGDSYVD GNN
Sbjct: 1   MKLPNLGFCFFFFFI--LFALQTASPTFPEAS--TISLP---NCFGFFLFGDSYVDAGNN 60

Query: 61  NYINTTSDFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLIPPYLDPHNNFYEI 120
           NYINTT+DFQANFPPYG++FFH+PTGRFTDGRTI DFL EYAGLPLIPPYLDPHN+FY+ 
Sbjct: 61  NYINTTADFQANFPPYGKTFFHLPTGRFTDGRTIPDFLAEYAGLPLIPPYLDPHNDFYDN 120

Query: 121 GVNFASGGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNARAQSFLSNSVFFFNFG 180
           GVNFAS G+GALA+SH  SAIGLQTQMKFFKKV+KSLRKKLGNA AQ+ +SNSVF FNFG
Sbjct: 121 GVNFASSGSGALAESHKGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSVFLFNFG 180

Query: 181 GNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGRKFGFMTVPPLGYMPN 240
           GNDYLNPFDISYDIFNT +AQE+FVNMV+GN++IALKEVYKYGGRKFGFMTVPPLG+MP+
Sbjct: 181 GNDYLNPFDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPS 240

Query: 241 SRMKKSVQFFEEASSIARLHNKLLPDAVHKLAIQLKGFKYAFADTHTLLLQRILNPTEYG 300
           SR+K+SVQFFEEASSI RLHN+LLPDA+ KL IQLKGFKYAFADTHTLLLQRI+NP +YG
Sbjct: 241 SRIKRSVQFFEEASSITRLHNQLLPDALQKLGIQLKGFKYAFADTHTLLLQRIINPIKYG 300

Query: 301 LKIVETACCGSEEFKGFYNCGRKRGSLPFTHCDNLEDYMFYDSFHPTEKVFKQIAQLMWS 360
            K+ ETACCGS  F+G YNCGR    LPFT CDNLEDY+F+DSFHPTEK+FKQ+A+L+WS
Sbjct: 301 FKVTETACCGSGAFRGIYNCGRNVEGLPFTECDNLEDYVFFDSFHPTEKLFKQLAELIWS 360

Query: 361 GDAEIVKPCNFKQLFFQNDPMLASH 386
           GDA+IV P NFKQL F+   MLAS+
Sbjct: 361 GDAQIVNPYNFKQL-FEYGSMLASY 377

BLAST of Tan0020457 vs. ExPASy TrEMBL
Match: A0A6J1EIS0 (GDSL esterase/lipase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111434936 PE=3 SV=1)

HSP 1 Score: 618.2 bits (1593), Expect = 2.3e-173
Identity = 301/385 (78.18%), Postives = 330/385 (85.71%), Query Frame = 0

Query: 1   MKFLNLGICFVFLFFSNLFAAQSAFPTITEASRNQVK-LPWSKKRFGFFIFGDSYVDVGN 60
           MKF NLG CF F+    LFAAQ  FPTI+EA   Q+   P S+ RFGFFIFGDSYVDVGN
Sbjct: 1   MKFPNLGSCFFFI----LFAAQCGFPTISEAVGIQLPGAPSSENRFGFFIFGDSYVDVGN 60

Query: 61  NNYINTTSDFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLIPPYLDPHNNFYE 120
           NNYINTTSDFQANFPPYGESFF + TGRFTDGR I DFLGEYA LPL+PPYLDPHN+ Y+
Sbjct: 61  NNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYD 120

Query: 121 IGVNFASGGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNARAQSFLSNSVFFFNF 180
            GVNFASGG GALA SH E A+GLQTQMKFFK+V KSLRKKLGNAR QSF SNSVF FNF
Sbjct: 121 YGVNFASGGGGALALSHQEQALGLQTQMKFFKRVDKSLRKKLGNARTQSFFSNSVFLFNF 180

Query: 181 GGNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGRKFGFMTVPPLGYMP 240
           GGNDYLNPFDISYDIF T++AQEQFVNMVIGN++ ALKEVYK+GGRKFG M VPPLGYMP
Sbjct: 181 GGNDYLNPFDISYDIFKTIDAQEQFVNMVIGNITKALKEVYKHGGRKFGLMGVPPLGYMP 240

Query: 241 NSRMKKSVQFFEEASSIARLHNKLLPDAVHKLAIQLKGFKYAFADTHTLLLQRILNPTEY 300
           +SR+KKS QFFEEASSIARLHNKLLP A+HKL+ QLKGFKYAFADTHTLLLQRILNP+ Y
Sbjct: 241 SSRLKKSAQFFEEASSIARLHNKLLPIALHKLSKQLKGFKYAFADTHTLLLQRILNPSRY 300

Query: 301 GLKIVETACCGSEEFKGFYNCGRKRGSLPFTHCDNLEDYMFYDSFHPTEKVFKQIAQLMW 360
           G K+V+TACCGS+EF+G YNCGRK GSLPFTHC NLED+MF+DSFHPTEKVFKQ+ + MW
Sbjct: 301 GFKVVDTACCGSDEFRGVYNCGRKLGSLPFTHCKNLEDHMFFDSFHPTEKVFKQLTEQMW 360

Query: 361 SGDAEIVKPCNFKQLFFQNDPMLAS 385
           SG  E+VKP NFKQLF      LAS
Sbjct: 361 SGGLEVVKPYNFKQLFDYEPTTLAS 381

BLAST of Tan0020457 vs. ExPASy TrEMBL
Match: A0A6J1G6L0 (GDSL esterase/lipase 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111451341 PE=3 SV=1)

HSP 1 Score: 609.4 bits (1570), Expect = 1.1e-170
Identity = 295/385 (76.62%), Postives = 333/385 (86.49%), Query Frame = 0

Query: 1   MKFLNLGICFVFLFFSNLFAAQSAFPTITEASRNQVKLPWSKKRFGFFIFGDSYVDVGNN 60
           MK  NLG CF F F   LFA  +A PT  EA+   + LP     F FF+FGDSYVD GNN
Sbjct: 1   MKLPNLGFCFFFFFI--LFALHTASPTFPEAT--TIPLP---NCFAFFLFGDSYVDAGNN 60

Query: 61  NYINTTSDFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLIPPYLDPHNNFYEI 120
           NYINTT+DFQANFPPYGE+FFH+PT RFTDGRTI DFL EYAGLPLIPPYLDPH NFY+ 
Sbjct: 61  NYINTTADFQANFPPYGETFFHLPTRRFTDGRTIPDFLAEYAGLPLIPPYLDPHTNFYDN 120

Query: 121 GVNFASGGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNARAQSFLSNSVFFFNFG 180
           GVNFAS G+GALA+SH  SAIGLQTQMKFFKKV+KSLRKKLGNA AQ+ +SNS+F FNFG
Sbjct: 121 GVNFASSGSGALAESHEGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSIFLFNFG 180

Query: 181 GNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGRKFGFMTVPPLGYMPN 240
           GNDYLNPFDISYDIFNT +AQE+FVNMV+GN++IALKEVYKYGGRKFGFMTVPPLG+MP+
Sbjct: 181 GNDYLNPFDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPS 240

Query: 241 SRMKKSVQFFEEASSIARLHNKLLPDAVHKLAIQLKGFKYAFADTHTLLLQRILNPTEYG 300
           SR+K+SVQFFEEASSI RLHN+LLPDA+ KL IQL+GFKYAFADTHTLLLQRILNP +YG
Sbjct: 241 SRIKRSVQFFEEASSITRLHNQLLPDALQKLGIQLEGFKYAFADTHTLLLQRILNPIKYG 300

Query: 301 LKIVETACCGSEEFKGFYNCGRKRGSLPFTHCDNLEDYMFYDSFHPTEKVFKQIAQLMWS 360
            K+VETACCGS  F+G YNCGR    LPFTHCDNLEDY+F+DSFHPTEK+FKQ+A+L+WS
Sbjct: 301 FKVVETACCGSGAFRGIYNCGRNVEGLPFTHCDNLEDYVFFDSFHPTEKLFKQLAELIWS 360

Query: 361 GDAEIVKPCNFKQLFFQNDPMLASH 386
           GDA+IVKP NFKQL F+ D MLAS+
Sbjct: 361 GDAQIVKPYNFKQL-FEYDSMLASY 377

BLAST of Tan0020457 vs. ExPASy TrEMBL
Match: A0A6J1L1A1 (GDSL esterase/lipase 2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499513 PE=3 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 1.4e-170
Identity = 295/385 (76.62%), Postives = 333/385 (86.49%), Query Frame = 0

Query: 1   MKFLNLGICFVFLFFSNLFAAQSAFPTITEASRNQVKLPWSKKRFGFFIFGDSYVDVGNN 60
           MK  NLG CF F F   LFA Q+A PT  EAS   + LP     FGFF+FGDSYVD GNN
Sbjct: 1   MKLPNLGFCFFFFFI--LFALQTASPTFPEAS--TISLP---NCFGFFLFGDSYVDAGNN 60

Query: 61  NYINTTSDFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLIPPYLDPHNNFYEI 120
           NYINTT+DFQANFPPYG++FFH+PTGRFTDGRTI DFL EYAGLPLIPPYLDPHN+FY+ 
Sbjct: 61  NYINTTADFQANFPPYGKTFFHLPTGRFTDGRTIPDFLAEYAGLPLIPPYLDPHNDFYDN 120

Query: 121 GVNFASGGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNARAQSFLSNSVFFFNFG 180
           GVNFAS G+GALA+SH  SAIGLQTQMKFFKKV+KSLRKKLGNA AQ+ +SNSVF FNFG
Sbjct: 121 GVNFASSGSGALAESHKGSAIGLQTQMKFFKKVMKSLRKKLGNASAQTLISNSVFLFNFG 180

Query: 181 GNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGRKFGFMTVPPLGYMPN 240
           GNDYLNPFDISYDIFNT +AQE+FVNMV+GN++IALKEVYKYGGRKFGFMTVPPLG+MP+
Sbjct: 181 GNDYLNPFDISYDIFNTSSAQERFVNMVVGNITIALKEVYKYGGRKFGFMTVPPLGHMPS 240

Query: 241 SRMKKSVQFFEEASSIARLHNKLLPDAVHKLAIQLKGFKYAFADTHTLLLQRILNPTEYG 300
           SR+K+SVQFFEEASSI RLHN+LLPDA+ KL IQLKGFKYAFADTHTLLLQRI+NP +YG
Sbjct: 241 SRIKRSVQFFEEASSITRLHNQLLPDALQKLGIQLKGFKYAFADTHTLLLQRIINPIKYG 300

Query: 301 LKIVETACCGSEEFKGFYNCGRKRGSLPFTHCDNLEDYMFYDSFHPTEKVFKQIAQLMWS 360
            K+ ETACCGS  F+G YNCGR    LPFT CDNLEDY+F+DSFHPTEK+FKQ+A+L+WS
Sbjct: 301 FKVTETACCGSGAFRGIYNCGRNVEGLPFTECDNLEDYVFFDSFHPTEKLFKQLAELIWS 360

Query: 361 GDAEIVKPCNFKQLFFQNDPMLASH 386
           GDA+IV P NFKQL F+   MLAS+
Sbjct: 361 GDAQIVNPYNFKQL-FEYGSMLASY 377

BLAST of Tan0020457 vs. ExPASy TrEMBL
Match: A0A6J1I9A0 (GDSL esterase/lipase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470360 PE=3 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 7.1e-167
Identity = 288/385 (74.81%), Postives = 323/385 (83.90%), Query Frame = 0

Query: 1   MKFLNLGICFVFLFFSNLFAAQSAFPTITEASRNQVK-LPWSKKRFGFFIFGDSYVDVGN 60
           MKF N   CF F+    LF AQ  FPTI+EA   Q+   P S+ RFGFFIFGDSYVD GN
Sbjct: 1   MKFPNRSSCFFFI----LFVAQCGFPTISEAVGIQLTGAPSSENRFGFFIFGDSYVDAGN 60

Query: 61  NNYINTTSDFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLIPPYLDPHNNFYE 120
           NNYINTTSDFQANFPPYGESFF + TGRFTDGR I DFLGEYA LPL+PPYLDPHN+ Y+
Sbjct: 61  NNYINTTSDFQANFPPYGESFFPVATGRFTDGRNIPDFLGEYANLPLLPPYLDPHNDLYD 120

Query: 121 IGVNFASGGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNARAQSFLSNSVFFFNF 180
            GVNFASGG GAL  SH E A+GLQTQM FFK+V KSLRKKLGNAR QSF SNSVF FNF
Sbjct: 121 YGVNFASGGGGALPLSHQEQALGLQTQMNFFKRVDKSLRKKLGNARTQSFFSNSVFLFNF 180

Query: 181 GGNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGRKFGFMTVPPLGYMP 240
           GGNDYLNPFDISYDIF T++ QEQFVNMVIGN++  LKEVYK+GGRKFG M VPPLGYMP
Sbjct: 181 GGNDYLNPFDISYDIFKTIDTQEQFVNMVIGNITTVLKEVYKHGGRKFGLMGVPPLGYMP 240

Query: 241 NSRMKKSVQFFEEASSIARLHNKLLPDAVHKLAIQLKGFKYAFADTHTLLLQRILNPTEY 300
           +SR+KKS Q+FEEASSIARLHNKLLP A+HKL+ QLKGFKYAFAD H LLLQRILNP++Y
Sbjct: 241 SSRLKKSAQYFEEASSIARLHNKLLPIALHKLSKQLKGFKYAFADVHMLLLQRILNPSQY 300

Query: 301 GLKIVETACCGSEEFKGFYNCGRKRGSLPFTHCDNLEDYMFYDSFHPTEKVFKQIAQLMW 360
           G K+V+TACCGS+EF+G YNCGRK GS PFTHC N+ED+MF+DS+HPTEKVFKQ+A+ MW
Sbjct: 301 GFKVVDTACCGSDEFRGVYNCGRKLGSFPFTHCKNIEDHMFFDSYHPTEKVFKQLAEQMW 360

Query: 361 SGDAEIVKPCNFKQLFFQNDPMLAS 385
           SG  E+V P NFKQL F+ +P LAS
Sbjct: 361 SGGLEVVTPYNFKQL-FEYEPTLAS 380

BLAST of Tan0020457 vs. ExPASy TrEMBL
Match: A0A0A0LQ38 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G296110 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 6.0e-158
Identity = 276/390 (70.77%), Postives = 317/390 (81.28%), Query Frame = 0

Query: 1   MKFLNLGICFVFLFFSNLFAAQSAFPTITEASRNQVKLPWSKK---RFGFFIFGDSYVDV 60
           M+F N  IC  F+FF+           + EA+R  ++LP +      FGFFIFGDSYVD 
Sbjct: 1   MEFSNFCICCFFIFFA----------AVLEAAR--IQLPGNSSPDTSFGFFIFGDSYVDA 60

Query: 61  GNNNYINTTSDFQANFPPYGESFFHIP--TGRFTDGRTITDFLGEYAGLPLIPPYLDPHN 120
           GNNNYI TTSDFQANFPPYGESFF  P  TGRFTDGR I DFLGEYA LPLIPPYLDPHN
Sbjct: 61  GNNNYIITTSDFQANFPPYGESFFPNPIATGRFTDGRNIPDFLGEYANLPLIPPYLDPHN 120

Query: 121 NFYEIGVNFASGGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNARAQSFLSNSVF 180
           + Y+ G NFASGG GA+A SH E AIGLQTQM+FF+KV KSLR KLG+AR++SFLSNSVF
Sbjct: 121 DLYDYGANFASGGGGAIAMSHQEQAIGLQTQMEFFRKVEKSLRNKLGHARSKSFLSNSVF 180

Query: 181 FFNFGGNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGRKFGFMTVPPL 240
            FNFGGNDYLNPFDISYDIF T+ AQEQFVNMV+GN++IA+KEVY+YGGRKFG + VPPL
Sbjct: 181 LFNFGGNDYLNPFDISYDIFKTIEAQEQFVNMVVGNITIAIKEVYEYGGRKFGVLAVPPL 240

Query: 241 GYMPNSRMKKSVQFFEEASSIARLHNKLLPDAVHKLAIQLKGFKYAFADTHTLLLQRILN 300
           GYMP+SR+KKS QFFEEASSIAR+HNK L  A+ KL+ QLKGFKY FAD HT LLQRI N
Sbjct: 241 GYMPSSRLKKSAQFFEEASSIARIHNKFLLIALEKLSKQLKGFKYTFADVHTALLQRIQN 300

Query: 301 PTEYGLKIVETACCGSEEFKGFYNCGRKRGSLPFTHCDNLEDYMFYDSFHPTEKVFKQIA 360
           PTEYG K+V+TACCGS+EF+G YNCGR+ GS P+THC NLED+MF+DSFHPT+KVFKQ+A
Sbjct: 301 PTEYGFKVVDTACCGSDEFRGIYNCGREFGSSPYTHCQNLEDHMFFDSFHPTQKVFKQLA 360

Query: 361 QLMWSGDAEIVKPCNFKQLFFQNDPMLASH 386
              WSGD +IVKP NFKQLF  +D  LAS+
Sbjct: 361 DEFWSGDEDIVKPVNFKQLFHYDDSTLASY 378

BLAST of Tan0020457 vs. TAIR 10
Match: AT1G53990.1 (GDSL-motif lipase 3 )

HSP 1 Score: 295.8 bits (756), Expect = 5.0e-80
Identity = 154/334 (46.11%), Postives = 209/334 (62.57%), Query Frame = 0

Query: 48  FIFGDSYVDVGNNNYINTTSDFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLI 107
           F+FGDS  D GNNNYINT S F++N  PYG++ F  PTGR +DG        E A LP I
Sbjct: 37  FVFGDSLFDAGNNNYINTVSSFRSNIWPYGQTNFKFPTGRLSDG-------PEKAWLPSI 96

Query: 108 PPYLDPH--NNFYEIGVNFASGGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNAR 167
           PP L P+  NN +  GV+FAS GAGALA+S     I L TQ+  FK V KSLR +LG+A 
Sbjct: 97  PPNLQPNNGNNQFTYGVSFASAGAGALAESFLGMVINLGTQLNNFKDVEKSLRSELGDAE 156

Query: 168 AQSFLSNSVFFFNFGGNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGR 227
            +   S +V+ F+ G NDY  PF  +   F + N++E+FV+ VIGN++  ++EVYK GGR
Sbjct: 157 TKRVFSRAVYLFHIGANDYFYPFSANSSTFKS-NSKEKFVDFVIGNITFVIEEVYKMGGR 216

Query: 228 KFGFMTVPPLGYMPNSRMKKSVQF---FEEASSIARLHNKLLPDAVHKLAIQLKGFKYAF 287
           KFGF+ V P    PNS ++   +    F+  + +  +HNK  PD + +L  QL GF+YA 
Sbjct: 217 KFGFLNVGPYECSPNSLIRDRTKIGSCFKPVAELIDMHNKKFPDVLRRLQRQLSGFRYAL 276

Query: 288 ADTHTLLLQRILNPTEYGLKIVETACCGSEEFKGFYNCGRKRG-SLPFTHCDNLEDYMFY 347
            D HT L +RI +P++YG K  + ACCGS   +G   CG + G S  +  C+N+ DY+FY
Sbjct: 277 HDYHTSLSERINSPSKYGFKEGKKACCGSGPLRGINTCGNRIGPSQGYGLCENVTDYLFY 336

Query: 348 DSFHPTEKVFKQIAQLMWSGDAEIVKPCNFKQLF 376
           DS H TEK  +QIA+L+W+G   + +P N K LF
Sbjct: 337 DSSHLTEKAHRQIAELIWNGPPNVTRPYNLKALF 362

BLAST of Tan0020457 vs. TAIR 10
Match: AT1G53920.1 (GDSL-motif lipase 5 )

HSP 1 Score: 294.7 bits (753), Expect = 1.1e-79
Identity = 155/376 (41.22%), Postives = 234/376 (62.23%), Query Frame = 0

Query: 10  FVFLFFSN--LFAAQSAFPTITEASRNQVKLPWSKKRFGFFIFGDSYVDVGNNNYINTTS 69
           F+F   S+  LF A  +   I+    N V           F+FGDS++D GNNNYINTT+
Sbjct: 18  FIFFIVSSTILFLAGKSSAKISHNGDNNVT--------ALFLFGDSFLDAGNNNYINTTT 77

Query: 70  DFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLIPPYLDPHNNFYEI-GVNFAS 129
             QANFPPYG++FF +PTGRF+DGR I+DF+ EYA LPLIPP+L+P N+  ++ GVNFAS
Sbjct: 78  LDQANFPPYGQTFFGLPTGRFSDGRLISDFIAEYANLPLIPPFLEPGNSQKKLYGVNFAS 137

Query: 130 GGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNARAQSFLSNSVFFFNFGGNDYLN 189
            GAGAL ++   S I L+TQ+  +KKV +  R   G   ++  +S +V+  + G NDY +
Sbjct: 138 AGAGALVETFQGSVINLRTQLDHYKKVERLWRTNFGKEESKKRISRAVYLISIGSNDYSS 197

Query: 190 PFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGRKFGFMTVPPLGYMPNSRM--- 249
            F  +  +  +++   Q V++VIGN++  + E+YK GGRKFGF+ VP LG  P  R+   
Sbjct: 198 IFLTNQSLPISMS---QHVDIVIGNLTTFIHEIYKIGGRKFGFLNVPDLGCFPALRILQP 257

Query: 250 KKSVQFFEEASSIARLHNKLLPDAVHKLAIQLKGFKYAFADTHTLLLQRILNPTEYGLKI 309
           K       +AS +A +HN+ L + + ++  Q+KGFK++  D +  L  R+ +P+++G K 
Sbjct: 258 KNDDSCLRDASRLASMHNRALTNLLFQMQRQVKGFKFSLFDMNKSLRLRMQHPSKFGFKE 317

Query: 310 VETACCGSEEFKGFYNCGRKRGSLPFTHCDNLEDYMFYDSFHPTEKVFKQIAQLMWSG-- 369
            E ACCG+ +++G ++CG KR    +  C+N +DY+F+DS H T+  + Q A L+W+G  
Sbjct: 318 GEEACCGTGKWRGVFSCGGKRIVKEYQLCENPKDYIFWDSLHLTQNTYNQFANLIWNGGH 377

Query: 370 --DAEIVKPCNFKQLF 376
             D+ +V P N   LF
Sbjct: 378 MSDSLVVGPYNINNLF 382

BLAST of Tan0020457 vs. TAIR 10
Match: AT5G40990.1 (GDSL lipase 1 )

HSP 1 Score: 294.7 bits (753), Expect = 1.1e-79
Identity = 152/337 (45.10%), Postives = 210/337 (62.31%), Query Frame = 0

Query: 48  FIFGDSYVDVGNNNYINTTSDFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLI 107
           F+FGDS  D GNNNYI+T S  ++N+ PYG++ F  PTGR +DGR I DF+ EYA LPLI
Sbjct: 39  FVFGDSVFDAGNNNYIDTLSSVRSNYWPYGQTTFKSPTGRVSDGRLIPDFIAEYAWLPLI 98

Query: 108 PPYLDPH--NNFYEIGVNFASGGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNAR 167
           PP L P   N+ +  GVNFASGGAGAL  +     I L+TQ+  FKKV + LR KLG+A 
Sbjct: 99  PPNLQPFNGNSQFAYGVNFASGGAGALVGTFSGLVINLRTQLNNFKKVEEMLRSKLGDAE 158

Query: 168 AQSFLSNSVFFFNFGGNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGR 227
            +  +S +V+ F+ G NDY  PF  +  +F ++ + E++V+ V+GNM+   KEVY  GGR
Sbjct: 159 GKRVISRAVYLFHIGLNDYQYPFTTNSSLFQSI-SNEKYVDYVVGNMTDVFKEVYNLGGR 218

Query: 228 KFGFMTVPPLGYMPNSRM---KKSVQFFEEASSIARLHNKLLPDAVHKLAIQLKGFKYAF 287
           KFG +   P    P S +    K    F+  + +  +HN+ L + + +L  +L GFKYA 
Sbjct: 219 KFGILNTGPYDCAPASLVIDQTKIRSCFQPVTELINMHNEKLLNGLRRLNHELSGFKYAL 278

Query: 288 ADTHTLLLQRILNPTEYGLKIVETACCGSEEFKGFYNCGRKRG-SLPFTHCDNLEDYMFY 347
            D HT L +R+ +P++YG K  + ACCGS   +G   CG + G S  +  C+N+ DY+F+
Sbjct: 279 HDYHTSLSERMNDPSKYGFKEGKKACCGSGPLRGINTCGGRMGLSQSYELCENVTDYLFF 338

Query: 348 DSFHPTEKVFKQIAQLMWSGDAEIVKPCNFKQLFFQN 379
           D FH TEK  +QIA+L+WSG   I  P N K LF  N
Sbjct: 339 DPFHLTEKANRQIAELIWSGPTNITGPYNLKALFELN 374

BLAST of Tan0020457 vs. TAIR 10
Match: AT1G53940.1 (GDSL-motif lipase 2 )

HSP 1 Score: 282.7 bits (722), Expect = 4.3e-76
Identity = 145/320 (45.31%), Postives = 200/320 (62.50%), Query Frame = 0

Query: 48  FIFGDSYVDVGNNNYINTTSDFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLI 107
           F+FGDS  D GNNNYI+T   F++N+ PYG++ F  PTGR +DGRTI DF+ EYA LPLI
Sbjct: 41  FVFGDSVFDAGNNNYIDTLPSFRSNYWPYGQTTFKFPTGRVSDGRTIPDFIAEYAWLPLI 100

Query: 108 PPYLDPHN--NFYEIGVNFASGGAGALAQSHGESAIGLQTQMKFFKKVVKSLRKKLGNAR 167
           P YL P N  N +  GV+FAS GAGAL  +     I L++Q+  FKKV K LR  LG A+
Sbjct: 101 PAYLQPSNGKNQFPYGVSFASAGAGALVGTFPGMVINLKSQLNNFKKVEKLLRSTLGEAQ 160

Query: 168 AQSFLSNSVFFFNFGGNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYKYGGR 227
            +  +S +V+ F+ G NDY  PF  +  IF + + QE +V+ V+GN +  +KEVYK GGR
Sbjct: 161 GKMVISRAVYLFHIGVNDYQYPFSTNSSIFQS-SPQEIYVDFVVGNTTAVIKEVYKIGGR 220

Query: 228 KFGFMTVPPLGYMPNSRMKKSVQF---FEEASSIARLHNKLLPDAVHKLAIQLKGFKYAF 287
           KFGF+ +      P S +    +    F+  + +  LHN+ L   + +L  +L GFKYA 
Sbjct: 221 KFGFLNMGAYDCAPASLIIDQTKIGTCFKPVTELINLHNEKLESGLRRLERELSGFKYAL 280

Query: 288 ADTHTLLLQRILNPTEYGLKIVETACCGSEEFKGFYNCGRKRG-SLPFTHCDNLEDYMFY 347
            D HT L  R+ NP++YG K  + ACCG+   +G   CG + G S  +  C+ + DY+F+
Sbjct: 281 HDYHTSLSVRMNNPSKYGFKEGKMACCGTGPLRGINTCGGRMGVSQSYELCEKVTDYLFF 340

Query: 348 DSFHPTEKVFKQIAQLMWSG 362
           D FH TEK  +QIA+L+WSG
Sbjct: 341 DHFHLTEKAHQQIAELIWSG 359

BLAST of Tan0020457 vs. TAIR 10
Match: AT3G14225.1 (GDSL-motif lipase 4 )

HSP 1 Score: 280.8 bits (717), Expect = 1.7e-75
Identity = 146/338 (43.20%), Postives = 203/338 (60.06%), Query Frame = 0

Query: 48  FIFGDSYVDVGNNNYINTTSDFQANFPPYGESFFHIPTGRFTDGRTITDFLGEYAGLPLI 107
           F FGDS  + GNNNY ++ S F++NF PYG++ F  PTGR +DGR + DF+ EYA LPLI
Sbjct: 39  FAFGDSLFEAGNNNYFDSISSFRSNFWPYGKTTFKFPTGRVSDGRIMIDFIAEYAWLPLI 98

Query: 108 PPYLDP--HNNFYEIGVNFASGGAGALAQSHGESAIGLQ----TQMKFFKKVVKSLRKKL 167
           PP L P   N+    G+NFA+  AG  A +   S   L     TQ+  FK V K+LR  L
Sbjct: 99  PPNLQPGYSNSQLTYGLNFATTAAGVFAGTFPGSVTNLSKDLGTQLNNFKNVEKTLRSNL 158

Query: 168 GNARAQSFLSNSVFFFNFGGNDYLNPFDISYDIFNTLNAQEQFVNMVIGNMSIALKEVYK 227
           G+A A+  +S +V+ F+ G NDY  PF  +   F+    +E+F++ VIGN +  ++E+YK
Sbjct: 159 GDAEARRVISKAVYLFHIGANDYQYPFFANTSTFSN-TTKERFIDFVIGNTTTVIEELYK 218

Query: 228 YGGRKFGFMTVPPLGYMPNSRMKKSVQF---FEEASSIARLHNKLLPDAVHKLAIQLKGF 287
            G RKFGF+++ P G  P++ +  S +    FE  + +  LHN+  P  + +L  +L GF
Sbjct: 219 LGARKFGFLSLGPFGCTPSALIINSTKIGSCFEPVTELINLHNQEFPKVLRRLERRLSGF 278

Query: 288 KYAFADTHTLLLQRILNPTEYGLKIVETACCGSEEFKGFYNCGRKRG-SLPFTHCDNLED 347
           KYA  D HT L QRI NP+ YG K  E ACCGS   +G   CG + G S  +  C+N +D
Sbjct: 279 KYALHDFHTSLSQRINNPSRYGFKEGEMACCGSGPLRGINTCGFRNGPSQGYKLCENADD 338

Query: 348 YMFYDSFHPTEKVFKQIAQLMWSGDAEIVKPCNFKQLF 376
           Y+F+D  H TE   +QIA+L+WSG   +  P N K LF
Sbjct: 339 YVFFDPSHLTETAHQQIAELIWSGPPNVTAPYNLKTLF 375