Tan0020455 (gene) Snake gourd v1

BLAST of Tan0020455 vs. ExPASy Swiss-Prot
Match: Q948R1 (Phospholipase A(1) DAD1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DAD1 PE=1 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 4.6e-148
Identity = 270/383 (70.50%), Postives = 314/383 (81.98%), Query Frame = 0

Query: 50  ISGSLASTSGYVKVKLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDF 109
           I G  + T   + VKLG++W +YQGLQNW+GLLDPLDDNLR EILRYGQFV++AY++FDF
Sbjct: 71  IPGEFSPTGSSIPVKLGRRWMEYQGLQNWDGLLDPLDDNLRREILRYGQFVESAYQAFDF 130

Query: 110 DPSSPSYATSLHPKASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHKAPS-VAANSSWI 169
           DPSSP+Y T   P+++LLERSGLP++GYR++KNLRATSGI LP W+ KAPS +A  SSWI
Sbjct: 131 DPSSPTYGTCRFPRSTLLERSGLPNSGYRLTKNLRATSGINLPRWIEKAPSWMATQSSWI 190

Query: 170 GYVAVCHDKHEISRLGRRDIVIAFRGTATCLEWLENLRATLTKLPE---ESCCSGSDPMA 229
           GYVAVC DK EISRLGRRD+VI+FRGTATCLEWLENLRATLT LP     +  +GS+   
Sbjct: 191 GYVAVCQDKEEISRLGRRDVVISFRGTATCLEWLENLRATLTHLPNGPTGANLNGSNSGP 250

Query: 230 MVESGFLSLYTSESEWCPSLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLA 289
           MVESGFLSLYTS      SLR MVREEI+RLLQSYG+    PLS+TITGHSLGAA+ATLA
Sbjct: 251 MVESGFLSLYTSGVH---SLRDMVREEIARLLQSYGD---EPLSVTITGHSLGAAIATLA 310

Query: 290 AYDIKEYFKEKVGMVTVMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLK 349
           AYDIK  FK +  MVTV+SFGGPRVGNR FRK +EKQGTKVLRIVNSDDVITKVPG VL+
Sbjct: 311 AYDIKTTFK-RAPMVTVISFGGPRVGNRCFRKLLEKQGTKVLRIVNSDDVITKVPGVVLE 370

Query: 350 -EEAEGVRGGGRGVPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHLTGINVATCHDLNT 409
             E + V+     +P WI++ VEET  W Y+E+G+ELRLSSRDSPHL+ INVATCH+L T
Sbjct: 371 NREQDNVKMTASIMPSWIQRRVEETP-WVYAEIGKELRLSSRDSPHLSSINVATCHELKT 430

Query: 410 YLHLVDGFVSSTCPFRATARRIL 428
           YLHLVDGFVSSTCPFR TARR+L
Sbjct: 431 YLHLVDGFVSSTCPFRETARRVL 445

BLAST of Tan0020455 vs. ExPASy Swiss-Prot
Match: O23522 (Phospholipase A1-Ibeta2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g16820 PE=1 SV=2)

HSP 1 Score: 343.6 bits (880), Expect = 3.2e-93
Identity = 190/374 (50.80%), Postives = 236/374 (63.10%), Query Frame = 0

Query: 62  KVKLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDP-SSPSYATSL 121
           K KLG KW++  GL NW GLLDPLD+NLR E++RYG+FV AAY +F  DP  SP +    
Sbjct: 124 KSKLGSKWRELHGLNNWAGLLDPLDENLRRELVRYGEFVQAAYHAFHSDPEGSPRHV--- 183

Query: 122 HPKASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHK-APS---VAANSSWIGYVAVCHD 181
                      LP   ++V+K+L ATS +RLP W+   AP    +   +SW+GYVAVC D
Sbjct: 184 ----------ALPDGSFKVTKSLYATSSVRLPKWIDDVAPDLRWMTKQTSWVGYVAVCDD 243

Query: 182 KHEISRLGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYT 241
             EI R+GRR+IVIA RGTAT LEW EN R  L  +PE            VE GF SLYT
Sbjct: 244 PREIRRMGRREIVIALRGTATLLEWSENFRPNLVSMPEPKPDQSDPTRPKVECGFNSLYT 303

Query: 242 SESEWCPSLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEYFKEK 301
           +  +  PSL + +  EISRL++ Y  GE+  LS+++TGHSLGAA+A LAA DI E     
Sbjct: 304 TGDQHAPSLAESLVGEISRLVELYA-GEE--LSISVTGHSLGAAIALLAADDIAERVPH- 363

Query: 302 VGMVTVMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLKEEAEG-VRGGG 361
              V V SFGGPRVGNR F  R++ +G KVLR+VNS DV+TKVPG     + +G  R  G
Sbjct: 364 APPVAVFSFGGPRVGNREFADRLDSKGVKVLRVVNSQDVVTKVPGIFADNDKQGQSRNNG 423

Query: 362 RGVPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHL-TGINVATCHDLNTYLHLVDGFVS 421
           R  P  I + VE    WAYS VG ELR+  + SP+L    +VA CHDL  YLHLVDGF++
Sbjct: 424 RS-PGGIMEMVERNNPWAYSHVGAELRVDMKMSPYLKPNADVACCHDLEAYLHLVDGFLA 479

Query: 422 STCPFRATARRILR 429
           S CPFRA A+R LR
Sbjct: 484 SNCPFRANAKRSLR 479

BLAST of Tan0020455 vs. ExPASy Swiss-Prot
Match: Q3EBR6 (Phospholipase A1-Igamma2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL3 PE=1 SV=2)

HSP 1 Score: 278.1 bits (710), Expect = 1.7e-73
Identity = 154/362 (42.54%), Postives = 216/362 (59.67%), Query Frame = 0

Query: 64  KLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPSYATSLHPK 123
           +L   W+  QG  +W GL+DP+D  LR+E++RYG+   A Y +FDFDP+S    TS   +
Sbjct: 100 RLRDTWRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTR 159

Query: 124 ASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHK---APSVAANSSWIGYVAVCHDKHEI 183
               +  G+  +GY V++ L ATS I LP++  K   +   + N++W+GYVAV  D+   
Sbjct: 160 LEFFDSLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSR 219

Query: 184 SRLGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYTSESE 243
           +RLGRRDI IA+RGT T LEW+ +L+  L  + E       DP   VESGFL LYT +  
Sbjct: 220 NRLGRRDIAIAWRGTVTKLEWIADLKDYLKPVTENK-IRCPDPAVKVESGFLDLYTDKDT 279

Query: 244 WCP----SLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEY---- 303
            C     S R+ +  E+ RL++ +G+ +   LS+T+TGHSLG ALA L+AYDI E     
Sbjct: 280 TCKFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNR 339

Query: 304 -FKEKVGMVTVMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLKEEAEGV 363
             K KV  VTV+++GGPRVGN  FR+R+E+ G KV+R+VN  DV+ K PG  L E     
Sbjct: 340 SKKGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESR--- 399

Query: 364 RGGGRGVPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHL-TGINVATCHDLNTYLHLVD 413
                  P  + K + E   W YS VG EL L  ++SP L   ++V+T H+L   LHL+D
Sbjct: 400 -------PHALMK-IAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLD 449

BLAST of Tan0020455 vs. ExPASy Swiss-Prot
Match: Q941F1 (Phospholipase A1-Igamma1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL4 PE=1 SV=2)

HSP 1 Score: 262.3 bits (669), Expect = 9.5e-69
Identity = 153/363 (42.15%), Postives = 208/363 (57.30%), Query Frame = 0

Query: 64  KLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPSYATSLHPK 123
           +L   W+  QG  +W GL+DP+D  LR+E++RYG+   A Y +FDFDP S    +    +
Sbjct: 84  RLRDTWRKIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTR 143

Query: 124 ASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHK---APSVAANSSWIGYVAVCHDKHEI 183
             L +  G+  +GY V++ L ATS I LP++  K   +   + N++W+GYVAV  D    
Sbjct: 144 RHLFDSLGIIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEAT 203

Query: 184 -SRLGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYTSES 243
             RLGRRDI IA+RGT T LEW+ +L+  L K    +     DP    ESGFL LYT + 
Sbjct: 204 RCRLGRRDIAIAWRGTVTRLEWIADLKDFL-KPVSGNGFRCPDPAVKAESGFLDLYTDKD 263

Query: 244 EWCP----SLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEY--- 303
             C     S R+ V  E+ RL++ YG+ E   LS+T+TGHSLG ALA L+AYD+ E    
Sbjct: 264 TSCNFSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVN 323

Query: 304 --FKEKVGMVTVMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLKEEAEG 363
              K KV  VT  ++GGPRVGN  F++R+EK G KVLR+VN  DV+ K PG  L E A  
Sbjct: 324 RTRKGKVIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERA-- 383

Query: 364 VRGGGRGVPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHL-TGINVATCHDLNTYLHLV 413
                   PQ + K       W YS VG  L L  + SP L   ++++T H+L   LHL+
Sbjct: 384 --------PQALMKLAGGLP-WCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLL 434

BLAST of Tan0020455 vs. ExPASy Swiss-Prot
Match: Q9C8J6 (Phospholipase A1-Igamma3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DALL2 PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 8.1e-68
Identity = 156/369 (42.28%), Postives = 214/369 (57.99%), Query Frame = 0

Query: 62  KVKLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPSYAT-SL 121
           +V L + W++ QG  NWEG LDP++++LR EI+RYG+F  A Y SFDFDP S    +   
Sbjct: 84  EVSLREIWREVQGCNNWEGQLDPMNNHLRREIIRYGEFAQACYDSFDFDPHSKYCGSCKY 143

Query: 122 HPKASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHKA--PSV-AANSSWIGYVAVCHDK 181
           HP    L        GY +++ L ATS I LP++  K+   S+ + +++W+G+VAV  D+
Sbjct: 144 HPSDFFLNLDLHLHKGYTITRYLYATSNINLPNFFQKSKLSSIWSQHANWMGFVAVATDE 203

Query: 182 HEISRLGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYTS 241
            E+SRLGRRDIVIA+RGT T LEW+ +L+  L      S   G DP   +E GF  LYT 
Sbjct: 204 EEVSRLGRRDIVIAWRGTVTYLEWIYDLKDILC-----SANFGDDPSIKIELGFHDLYTK 263

Query: 242 ESEWCP----SLRQMVREEISRLLQSYG-EGEQPPLSLTITGHSLGAALATLAAYDIKEY 301
           + + C     S R+ V  E+ RL++ YG E E    S+T+TGHSLGA+LA ++AYDI E 
Sbjct: 264 KEDSCKFSSFSAREQVLAEVKRLIEYYGTEEEGHKTSITVTGHSLGASLALVSAYDIAEL 323

Query: 302 FKEKVG------MVTVMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLKE 361
               V        +TV SF GPRVGN  F++R ++ G KVLR+VN  D +  VPG    E
Sbjct: 324 NLNHVPENNYKIPITVFSFSGPRVGNLRFKERCDELGVKVLRVVNVHDKVPSVPGIFTNE 383

Query: 362 EAEGVRGGGRGVPQWIRKCVEE-TQY-WAYSEVGRELRLSSRDSPHLTGINVATC-HDLN 413
           + +             +K VEE T + W+Y+ VG EL L  + SP L       C H+L 
Sbjct: 384 KFQ------------FQKYVEEKTSFPWSYAHVGVELALDHKKSPFLKPTKDLGCAHNLE 435

BLAST of Tan0020455 vs. NCBI nr
Match: KAG7028476.1 (Phospholipase A(1) DAD1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 640.6 bits (1651), Expect = 9.8e-180
Identity = 334/439 (76.08%), Postives = 361/439 (82.23%), Query Frame = 0

Query: 1   MGFCGIRVAQNPSLPIPQH---KTAMRKSSYKSNWAGFENNESTTFGGASTSISGSLAST 60
           MG CGIRV QNP+L IP H    T MRK                      T+IS ++ S 
Sbjct: 1   MGLCGIRVPQNPTLSIPHHFHNNTHMRKP-------------------LCTAISAAVCSP 60

Query: 61  SGYVKVKLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPSYA 120
              VK+KLGK+W++YQGL NWEGLLDPLD+NLRNEILRYGQFVDAAYKSFDFDPSSPSYA
Sbjct: 61  C--VKLKLGKRWREYQGLHNWEGLLDPLDENLRNEILRYGQFVDAAYKSFDFDPSSPSYA 120

Query: 121 TSLHPKASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHKAPSVAANSSWIGYVAVCHDK 180
           T L+PKASLL+RSGLP+TGYRVSK+LRATSGIRLP WVHKAPSVA NSSWIGYVAVC DK
Sbjct: 121 TCLYPKASLLDRSGLPATGYRVSKHLRATSGIRLPGWVHKAPSVATNSSWIGYVAVCQDK 180

Query: 181 HEISRLGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYTS 240
            EISRLGRRD+VIAFRGTATCLEWLEN+RATLTKLP   C     P   VESGFLSLYTS
Sbjct: 181 QEISRLGRRDVVIAFRGTATCLEWLENVRATLTKLPSTGC----GPHMAVESGFLSLYTS 240

Query: 241 ESEWCPSLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEYFKEKV 300
           +++ CPSLRQMVR+EISRLLQSYG+    PLSLTITGHSLGAALATLAAYDIKEYFK + 
Sbjct: 241 DTDSCPSLRQMVRDEISRLLQSYGD---QPLSLTITGHSLGAALATLAAYDIKEYFK-RA 300

Query: 301 GMVTVMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLKEEAEGVRGG--- 360
            MVTVMSFGGPRVGN SFRKRVEKQGTKVLRIVNSDDVITKVPGFV+KEE E   GG   
Sbjct: 301 PMVTVMSFGGPRVGNSSFRKRVEKQGTKVLRIVNSDDVITKVPGFVVKEEEEEEEGGNAV 360

Query: 361 -----GRGVPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHLTGINVATCHDLNTYLHLV 420
                G GVP+WI+KCVE+TQ WAYS+VGRELRLSSRDSPHLTGIN ATCHDLNTYLHLV
Sbjct: 361 ASKLKGGGVPRWIQKCVEDTQ-WAYSDVGRELRLSSRDSPHLTGINAATCHDLNTYLHLV 409

Query: 421 DGFVSSTCPFRATARRILR 429
           DGFVSS+CPFRATARRILR
Sbjct: 421 DGFVSSSCPFRATARRILR 409

BLAST of Tan0020455 vs. NCBI nr
Match: KAG6597009.1 (Phospholipase A(1) DAD1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 639.8 bits (1649), Expect = 1.7e-179
Identity = 334/438 (76.26%), Postives = 364/438 (83.11%), Query Frame = 0

Query: 1   MGFCGIRVAQNPSLPIPQH---KTAMRKSSYKSNWAGFENNESTTFGGASTSISGSLAST 60
           MG CGIRV QNP+L IP H    T MRK                      T+IS +++S 
Sbjct: 1   MGLCGIRVPQNPTLSIPHHFHNNTHMRKP-------------------LCTAISAAVSSP 60

Query: 61  SGYVKVKLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPSYA 120
              VK+KLGK+W++YQGL NWEGLLDPLD+NLRNEILRYGQFVDAAYKSFDFDPSSPSYA
Sbjct: 61  C--VKLKLGKRWREYQGLHNWEGLLDPLDENLRNEILRYGQFVDAAYKSFDFDPSSPSYA 120

Query: 121 TSLHPKASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHKAPSVAANSSWIGYVAVCHDK 180
           T L+PKASLL+RSGLP+TGYRVSK+LRATSGIRLP WVHKAPSVA NSSWIGYVAVC DK
Sbjct: 121 TCLYPKASLLDRSGLPATGYRVSKHLRATSGIRLPGWVHKAPSVATNSSWIGYVAVCQDK 180

Query: 181 HEISRLGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYTS 240
            EISRLGRRD+VIAFRGTATCLEWLEN+RATLTKLP   C     P   VESGFLSLYTS
Sbjct: 181 QEISRLGRRDVVIAFRGTATCLEWLENVRATLTKLPSTGC----GPHMAVESGFLSLYTS 240

Query: 241 ESEWCPSLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEYFKEKV 300
           +++ CPSLRQMVR+EISRLLQSYG+    PLSLTITGHSLGAALATLAAYDIKEYFK + 
Sbjct: 241 DTDSCPSLRQMVRDEISRLLQSYGD---QPLSLTITGHSLGAALATLAAYDIKEYFK-RA 300

Query: 301 GMVTVMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLKEEAEG------- 360
            MVTVMSFGGPRVGN SFRKRVEKQGTKVLRIVNSDDVITKVPGFV+KEE EG       
Sbjct: 301 PMVTVMSFGGPRVGNSSFRKRVEKQGTKVLRIVNSDDVITKVPGFVVKEEEEGGNAVASK 360

Query: 361 VRGGGRGVPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHLTGINVATCHDLNTYLHLVD 420
           ++GG  GVP+WI+KCVE+TQ WAYS+VGRELRLSSRDSPHLTGIN ATCHDLNTYLHLVD
Sbjct: 361 LKGG--GVPRWIQKCVEDTQ-WAYSDVGRELRLSSRDSPHLTGINAATCHDLNTYLHLVD 406

Query: 421 GFVSSTCPFRATARRILR 429
           GFVSS+CPFRATARRILR
Sbjct: 421 GFVSSSCPFRATARRILR 406

BLAST of Tan0020455 vs. NCBI nr
Match: XP_022974260.1 (phospholipase A(1) DAD1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 637.1 bits (1642), Expect = 1.1e-178
Identity = 334/438 (76.26%), Postives = 360/438 (82.19%), Query Frame = 0

Query: 1   MGFCGIRVAQNPSLPIPQH---KTAMRKSSYKSNWAGFENNESTTFGGASTSISGSLAST 60
           MG CGIRV QNP+L IP H    T MRK                      T+IS +++S 
Sbjct: 1   MGLCGIRVPQNPTLSIPHHFHNNTHMRKP-------------------LCTAISAAVSSP 60

Query: 61  SGYVKVKLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPSYA 120
              VK+KLGK+W++YQGL NWEGLLDPLD+NLRNEILRYGQFVDAAYKSFDFDPSSP+YA
Sbjct: 61  C--VKLKLGKRWREYQGLHNWEGLLDPLDENLRNEILRYGQFVDAAYKSFDFDPSSPTYA 120

Query: 121 TSLHPKASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHKAPSVAANSSWIGYVAVCHDK 180
           T L+PKASLL+RSGLP+TGYRVSK+LRATSGIRLP WVHKAPSVA NSSWIGYVAVC DK
Sbjct: 121 TCLYPKASLLDRSGLPATGYRVSKHLRATSGIRLPGWVHKAPSVATNSSWIGYVAVCQDK 180

Query: 181 HEISRLGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYTS 240
            EISRLGRRD+VIAFRGTATCLEWLEN+RATLTKLP   C     P   VESGFLSLYTS
Sbjct: 181 QEISRLGRRDVVIAFRGTATCLEWLENVRATLTKLPSTGC----GPHMAVESGFLSLYTS 240

Query: 241 ESEWCPSLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEYFKEKV 300
            ++ CPSLRQMVREEISRLLQSYG+    PLSLTITGHSLGAALATLAAYDIKEYFK + 
Sbjct: 241 GTDSCPSLRQMVREEISRLLQSYGD---QPLSLTITGHSLGAALATLAAYDIKEYFK-RA 300

Query: 301 GMVTVMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLKEEAEGVRG---- 360
            MVTVMSFGGPRVGN SFRKRVEKQGTKVLRIVNSDDVITKVPGFV+KEE E   G    
Sbjct: 301 PMVTVMSFGGPRVGNSSFRKRVEKQGTKVLRIVNSDDVITKVPGFVVKEEEEEEGGHAVA 360

Query: 361 ---GGRGVPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHLTGINVATCHDLNTYLHLVD 420
               G GVP+WI+KCVE+TQ WAYSEVGRELRLSSRDSPHLTGIN ATCHDLNTYLHLVD
Sbjct: 361 SKLKGGGVPRWIQKCVEDTQ-WAYSEVGRELRLSSRDSPHLTGINAATCHDLNTYLHLVD 408

Query: 421 GFVSSTCPFRATARRILR 429
           GFVSS+CPFRATARRILR
Sbjct: 421 GFVSSSCPFRATARRILR 408

BLAST of Tan0020455 vs. NCBI nr
Match: XP_023539437.1 (phospholipase A(1) DAD1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 634.8 bits (1636), Expect = 5.4e-178
Identity = 331/438 (75.57%), Postives = 360/438 (82.19%), Query Frame = 0

Query: 1   MGFCGIRVAQNPSLPIPQH---KTAMRKSSYKSNWAGFENNESTTFGGASTSISGSLAST 60
           MG CGIRV QNP L IP H    T MRK                      T+IS +++S 
Sbjct: 1   MGLCGIRVPQNPRLWIPHHFHNNTHMRKP-------------------MCTAISAAVSSP 60

Query: 61  SGYVKVKLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPSYA 120
              VK+KLGK+W++YQGL NWEGLLDPLD+NLRNEILRYGQFVDAAYKSFDFDPSSPSYA
Sbjct: 61  C--VKLKLGKRWREYQGLHNWEGLLDPLDENLRNEILRYGQFVDAAYKSFDFDPSSPSYA 120

Query: 121 TSLHPKASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHKAPSVAANSSWIGYVAVCHDK 180
           T L+PKASLL+RSGLP+TGYRVSK+LRATSGIRLP WVHKAPSVA NSSWIGYVAVC DK
Sbjct: 121 TCLYPKASLLDRSGLPATGYRVSKHLRATSGIRLPGWVHKAPSVATNSSWIGYVAVCQDK 180

Query: 181 HEISRLGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYTS 240
            EISRLGRRD+VIAFRGTATCLEWLEN+RATLTKLP   C     P   VESGFLSLYTS
Sbjct: 181 QEISRLGRRDVVIAFRGTATCLEWLENVRATLTKLPSTGC----GPHMAVESGFLSLYTS 240

Query: 241 ESEWCPSLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEYFKEKV 300
           E++ CPSLRQMVR+EISRLLQSYG+    PLSLTITGHSLGAALATLAAYDIK+YFK + 
Sbjct: 241 ETDSCPSLRQMVRDEISRLLQSYGD---QPLSLTITGHSLGAALATLAAYDIKDYFK-RA 300

Query: 301 GMVTVMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLKEEAEGVRGG--- 360
            MVTVMSFGGPRVGN +FRKRVEKQGTKVLRIVNSDDVITKVPGFV+KEE E   G    
Sbjct: 301 PMVTVMSFGGPRVGNTTFRKRVEKQGTKVLRIVNSDDVITKVPGFVVKEEEEEEAGNAVA 360

Query: 361 ----GRGVPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHLTGINVATCHDLNTYLHLVD 420
               G GVP+WI+KCVE+T +WAYS+VGRELRLSSRDSPHLTGIN ATCHDLNTYLHLVD
Sbjct: 361 SKLKGGGVPRWIQKCVEDT-HWAYSDVGRELRLSSRDSPHLTGINAATCHDLNTYLHLVD 408

Query: 421 GFVSSTCPFRATARRILR 429
           GFVSS+CPFRATARRILR
Sbjct: 421 GFVSSSCPFRATARRILR 408

BLAST of Tan0020455 vs. NCBI nr
Match: XP_022975254.1 (phospholipase A(1) DAD1, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 630.6 bits (1625), Expect = 1.0e-176
Identity = 332/438 (75.80%), Postives = 358/438 (81.74%), Query Frame = 0

Query: 1   MGFCGIRVAQNPSLPIPQH---KTAMRKSSYKSNWAGFENNESTTFGGASTSISGSLAST 60
           MG CGIRV QNP+L IP H    T MRK                      T+IS +++S 
Sbjct: 1   MGLCGIRVPQNPTLSIPHHFHNNTHMRKP-------------------LCTAISAAVSSP 60

Query: 61  SGYVKVKLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPSYA 120
              VK+KLGK+W++YQGL NWEGLLDPLD+NLRNEILRYGQFVDAAYKSFDFDPSSP+YA
Sbjct: 61  C--VKLKLGKRWREYQGLHNWEGLLDPLDENLRNEILRYGQFVDAAYKSFDFDPSSPTYA 120

Query: 121 TSLHPKASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHKAPSVAANSSWIGYVAVCHDK 180
           T L+PKASLL+RSGLP+TGYRVSK+LRATSGIRLP WVHKAPSVA NSSWIGYVAVC DK
Sbjct: 121 TCLYPKASLLDRSGLPATGYRVSKHLRATSGIRLPGWVHKAPSVATNSSWIGYVAVCQDK 180

Query: 181 HEISRLGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYTS 240
            EISRLGRRD+VIAFRGTATCLEWLEN+RATLTKLP   C     P   VESGFLSLYTS
Sbjct: 181 QEISRLGRRDVVIAFRGTATCLEWLENVRATLTKLPSTGC----GPHMAVESGFLSLYTS 240

Query: 241 ESEWCPSLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEYFKEKV 300
            ++ CPSLRQMVREEISRLLQSYG+    PLSLTITGHSLGAALATLAAYDIKEYFK + 
Sbjct: 241 GTDSCPSLRQMVREEISRLLQSYGD---QPLSLTITGHSLGAALATLAAYDIKEYFK-RA 300

Query: 301 GMVTVMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLK--EEAEGVRGG- 360
            MVTVMSFGGPRVGN SFRKRVEKQGTKVLRIVNSDDVITKVPGFV+K  EE E   GG 
Sbjct: 301 PMVTVMSFGGPRVGNSSFRKRVEKQGTKVLRIVNSDDVITKVPGFVVKEEEEEEEEEGGH 360

Query: 361 -------GRGVPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHLTGINVATCHDLNTYLH 420
                  G GVP+WI+KCVE+TQ WAYSEVGRELRLSSRDSPHLTGIN ATCHDLNTYLH
Sbjct: 361 AVASKLKGGGVPRWIQKCVEDTQ-WAYSEVGRELRLSSRDSPHLTGINAATCHDLNTYLH 408

Query: 421 LVDGFVSSTCPFRATARR 426
           LVDGFVSS+CPFRATARR
Sbjct: 421 LVDGFVSSSCPFRATARR 408

BLAST of Tan0020455 vs. ExPASy TrEMBL
Match: A0A6J1IDH7 (phospholipase A(1) DAD1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111472900 PE=4 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 5.2e-179
Identity = 334/438 (76.26%), Postives = 360/438 (82.19%), Query Frame = 0

Query: 1   MGFCGIRVAQNPSLPIPQH---KTAMRKSSYKSNWAGFENNESTTFGGASTSISGSLAST 60
           MG CGIRV QNP+L IP H    T MRK                      T+IS +++S 
Sbjct: 1   MGLCGIRVPQNPTLSIPHHFHNNTHMRKP-------------------LCTAISAAVSSP 60

Query: 61  SGYVKVKLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPSYA 120
              VK+KLGK+W++YQGL NWEGLLDPLD+NLRNEILRYGQFVDAAYKSFDFDPSSP+YA
Sbjct: 61  C--VKLKLGKRWREYQGLHNWEGLLDPLDENLRNEILRYGQFVDAAYKSFDFDPSSPTYA 120

Query: 121 TSLHPKASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHKAPSVAANSSWIGYVAVCHDK 180
           T L+PKASLL+RSGLP+TGYRVSK+LRATSGIRLP WVHKAPSVA NSSWIGYVAVC DK
Sbjct: 121 TCLYPKASLLDRSGLPATGYRVSKHLRATSGIRLPGWVHKAPSVATNSSWIGYVAVCQDK 180

Query: 181 HEISRLGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYTS 240
            EISRLGRRD+VIAFRGTATCLEWLEN+RATLTKLP   C     P   VESGFLSLYTS
Sbjct: 181 QEISRLGRRDVVIAFRGTATCLEWLENVRATLTKLPSTGC----GPHMAVESGFLSLYTS 240

Query: 241 ESEWCPSLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEYFKEKV 300
            ++ CPSLRQMVREEISRLLQSYG+    PLSLTITGHSLGAALATLAAYDIKEYFK + 
Sbjct: 241 GTDSCPSLRQMVREEISRLLQSYGD---QPLSLTITGHSLGAALATLAAYDIKEYFK-RA 300

Query: 301 GMVTVMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLKEEAEGVRG---- 360
            MVTVMSFGGPRVGN SFRKRVEKQGTKVLRIVNSDDVITKVPGFV+KEE E   G    
Sbjct: 301 PMVTVMSFGGPRVGNSSFRKRVEKQGTKVLRIVNSDDVITKVPGFVVKEEEEEEGGHAVA 360

Query: 361 ---GGRGVPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHLTGINVATCHDLNTYLHLVD 420
               G GVP+WI+KCVE+TQ WAYSEVGRELRLSSRDSPHLTGIN ATCHDLNTYLHLVD
Sbjct: 361 SKLKGGGVPRWIQKCVEDTQ-WAYSEVGRELRLSSRDSPHLTGINAATCHDLNTYLHLVD 408

Query: 421 GFVSSTCPFRATARRILR 429
           GFVSS+CPFRATARRILR
Sbjct: 421 GFVSSSCPFRATARRILR 408

BLAST of Tan0020455 vs. ExPASy TrEMBL
Match: A0A6J1IJY2 (phospholipase A(1) DAD1, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111474376 PE=4 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 4.9e-177
Identity = 332/438 (75.80%), Postives = 358/438 (81.74%), Query Frame = 0

Query: 1   MGFCGIRVAQNPSLPIPQH---KTAMRKSSYKSNWAGFENNESTTFGGASTSISGSLAST 60
           MG CGIRV QNP+L IP H    T MRK                      T+IS +++S 
Sbjct: 1   MGLCGIRVPQNPTLSIPHHFHNNTHMRKP-------------------LCTAISAAVSSP 60

Query: 61  SGYVKVKLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPSYA 120
              VK+KLGK+W++YQGL NWEGLLDPLD+NLRNEILRYGQFVDAAYKSFDFDPSSP+YA
Sbjct: 61  C--VKLKLGKRWREYQGLHNWEGLLDPLDENLRNEILRYGQFVDAAYKSFDFDPSSPTYA 120

Query: 121 TSLHPKASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHKAPSVAANSSWIGYVAVCHDK 180
           T L+PKASLL+RSGLP+TGYRVSK+LRATSGIRLP WVHKAPSVA NSSWIGYVAVC DK
Sbjct: 121 TCLYPKASLLDRSGLPATGYRVSKHLRATSGIRLPGWVHKAPSVATNSSWIGYVAVCQDK 180

Query: 181 HEISRLGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYTS 240
            EISRLGRRD+VIAFRGTATCLEWLEN+RATLTKLP   C     P   VESGFLSLYTS
Sbjct: 181 QEISRLGRRDVVIAFRGTATCLEWLENVRATLTKLPSTGC----GPHMAVESGFLSLYTS 240

Query: 241 ESEWCPSLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEYFKEKV 300
            ++ CPSLRQMVREEISRLLQSYG+    PLSLTITGHSLGAALATLAAYDIKEYFK + 
Sbjct: 241 GTDSCPSLRQMVREEISRLLQSYGD---QPLSLTITGHSLGAALATLAAYDIKEYFK-RA 300

Query: 301 GMVTVMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLK--EEAEGVRGG- 360
            MVTVMSFGGPRVGN SFRKRVEKQGTKVLRIVNSDDVITKVPGFV+K  EE E   GG 
Sbjct: 301 PMVTVMSFGGPRVGNSSFRKRVEKQGTKVLRIVNSDDVITKVPGFVVKEEEEEEEEEGGH 360

Query: 361 -------GRGVPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHLTGINVATCHDLNTYLH 420
                  G GVP+WI+KCVE+TQ WAYSEVGRELRLSSRDSPHLTGIN ATCHDLNTYLH
Sbjct: 361 AVASKLKGGGVPRWIQKCVEDTQ-WAYSEVGRELRLSSRDSPHLTGINAATCHDLNTYLH 408

Query: 421 LVDGFVSSTCPFRATARR 426
           LVDGFVSS+CPFRATARR
Sbjct: 421 LVDGFVSSSCPFRATARR 408

BLAST of Tan0020455 vs. ExPASy TrEMBL
Match: A0A6J1GF22 (phospholipase A(1) DAD1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111453348 PE=4 SV=1)

HSP 1 Score: 611.3 bits (1575), Expect = 3.1e-171
Identity = 310/376 (82.45%), Postives = 333/376 (88.56%), Query Frame = 0

Query: 63  VKLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPSYATSLHP 122
           +KLGK+W++YQGL NWEGLLDPLD+NLRNEILRYGQFVDAAYKSFDFDPSSPSYAT L+P
Sbjct: 47  LKLGKRWREYQGLHNWEGLLDPLDENLRNEILRYGQFVDAAYKSFDFDPSSPSYATCLYP 106

Query: 123 KASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHKAPSVAANSSWIGYVAVCHDKHEISR 182
           KASLL+RSGLP+TGYRVSK+LRATSGIRLP WVHKAPSVA NSSWIGYVAVC DK EISR
Sbjct: 107 KASLLDRSGLPATGYRVSKHLRATSGIRLPGWVHKAPSVATNSSWIGYVAVCQDKQEISR 166

Query: 183 LGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYTSESEWC 242
           LGRRD+VIAFRGTATCLEWLEN+RATLTKLP   C     P   VESGFLSLYTS+++ C
Sbjct: 167 LGRRDVVIAFRGTATCLEWLENVRATLTKLPSTGC----GPHMAVESGFLSLYTSDTDSC 226

Query: 243 PSLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEYFKEKVGMVTV 302
           PSLRQMVR+EISRLLQSYG+    PLSLTITGHSLGAALATLAAYDIKEYFK +  MVTV
Sbjct: 227 PSLRQMVRDEISRLLQSYGD---QPLSLTITGHSLGAALATLAAYDIKEYFK-RAPMVTV 286

Query: 303 MSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLK--EEAEGVRGG------ 362
           MSFGGPRVGN SFRKRVEKQGTKVLRIVNSDDVITKVPGFV+K  EE E   GG      
Sbjct: 287 MSFGGPRVGNSSFRKRVEKQGTKVLRIVNSDDVITKVPGFVVKEEEEEEEEEGGNAVASK 346

Query: 363 --GRGVPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHLTGINVATCHDLNTYLHLVDGF 422
             G GVP+WI+KCVE+TQ WAYS+VGRELRLSSRDSPHLTGIN ATCHDLNTYLHLVDGF
Sbjct: 347 LKGGGVPRWIQKCVEDTQ-WAYSDVGRELRLSSRDSPHLTGINAATCHDLNTYLHLVDGF 406

Query: 423 VSSTCPFRATARRILR 429
           VSS+CPFRATARRILR
Sbjct: 407 VSSSCPFRATARRILR 413

BLAST of Tan0020455 vs. ExPASy TrEMBL
Match: A0A1S3AUS7 (phospholipase A(1) DAD1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103483103 PE=4 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 3.7e-164
Identity = 312/429 (72.73%), Postives = 346/429 (80.65%), Query Frame = 0

Query: 6   IRVAQNPSLPIPQHKTA-MRKS---SYKSNWAGFENNESTTFGGASTSISGSLASTSGYV 65
           +RVAQNP++PIP H+TA MRK      K +  G EN        +S+S +G  AS    +
Sbjct: 1   MRVAQNPNVPIPNHQTATMRKPVLYKTKLHMHGVENKLRCATSSSSSSSTG--ASDCLKL 60

Query: 66  KVKLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPSYATSLH 125
           +VK+GK+WK+Y GL NWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSP+YAT LH
Sbjct: 61  RVKVGKRWKEYAGLGNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPTYATCLH 120

Query: 126 PKASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHKAPSVAANSSWIGYVAVCHDKHEIS 185
            KASLLE SGLPSTGYRVSK+LRATSGI LP W+   PS + NSSWIGYVAV  DKHEIS
Sbjct: 121 SKASLLESSGLPSTGYRVSKHLRATSGICLPRWLPNTPSFSTNSSWIGYVAVSQDKHEIS 180

Query: 186 RLGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYTSESEW 245
           RLGRRD+VIA RGTATCLEWLENLRATLT LP      G +  AMVESGFLSLY+S +E 
Sbjct: 181 RLGRRDVVIALRGTATCLEWLENLRATLTMLP------GEEGGAMVESGFLSLYSSGTES 240

Query: 246 CPSLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEYFKEKVGMVT 305
           CPSL++MVREEI R+LQSYGE    PLSLTITGHSLGAALATLAAYDIKEYFK    MVT
Sbjct: 241 CPSLKEMVREEIGRILQSYGE---EPLSLTITGHSLGAALATLAAYDIKEYFKTSAPMVT 300

Query: 306 VMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLKEEAEG----VRGGGRG 365
           VMSFGGPRVGNR FR+R+E+QGTKVLRIVNS+DVITK+PGFV+            GGGR 
Sbjct: 301 VMSFGGPRVGNRKFRQRLEEQGTKVLRIVNSEDVITKLPGFVVNNNNNNNNNVEEGGGR- 360

Query: 366 VPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHLTGINVATCHDLNTYLHLVDGFVSSTC 425
             +WI+KCVEET+ WAYSEVGRELRLSSRDSPHL  INVATCH LNTYLHLVDGFVSSTC
Sbjct: 361 -LRWIQKCVEETE-WAYSEVGRELRLSSRDSPHLNRINVATCHHLNTYLHLVDGFVSSTC 415

Query: 426 PFRATARRI 427
           PFRATARR+
Sbjct: 421 PFRATARRM 415

BLAST of Tan0020455 vs. ExPASy TrEMBL
Match: A0A0A0L5L8 (Lipase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G116760 PE=4 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 1.2e-162
Identity = 313/431 (72.62%), Postives = 346/431 (80.28%), Query Frame = 0

Query: 6   IRVAQNPSLPIPQHKTA-MRKSSY---KSNWAGFENNESTTFGGASTSISGSLASTSGYV 65
           +RVAQNP++PIP H+TA MRK +    K +  G EN        +STS S + AS     
Sbjct: 1   MRVAQNPNVPIPNHQTATMRKPALYKNKLHMHGVEN--KLRCATSSTSSSSTGASECLKR 60

Query: 66  KVKLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPSYATSLH 125
           +VK+GK+WK+Y GL NWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSP+YAT LH
Sbjct: 61  RVKVGKRWKEYAGLGNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPTYATCLH 120

Query: 126 PKASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHKAPSVAANSSWIGYVAVCHDKHEIS 185
            KASLLE SGLPSTGYRVSK+LRATSGI LP W+  APS++ NSSWIGYVAV  DKHEIS
Sbjct: 121 SKASLLESSGLPSTGYRVSKHLRATSGICLPRWLRNAPSISTNSSWIGYVAVSQDKHEIS 180

Query: 186 RLGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYTSESEW 245
           RLGRRD+VI+ RGTATCLEWLENLRATLT LP      G +  AMVESGFLSLY+S +E 
Sbjct: 181 RLGRRDVVISLRGTATCLEWLENLRATLTTLP------GEEGGAMVESGFLSLYSSRTES 240

Query: 246 CPSLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEYFKEKVGMVT 305
            PSL++MVREEI RLLQSYGE     LSLTITGHSLGAALATLAAYDIKEYFK    MVT
Sbjct: 241 YPSLKEMVREEIGRLLQSYGE---EALSLTITGHSLGAALATLAAYDIKEYFKTSAPMVT 300

Query: 306 VMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLKEEAEG------VRGGG 365
           VMSFGGPRVGNR FR+R+EKQGTKVLRIVNS+DVITK+PGFV+   +          GGG
Sbjct: 301 VMSFGGPRVGNRKFRQRLEKQGTKVLRIVNSEDVITKLPGFVVNNSSSSSSNNNVEEGGG 360

Query: 366 RGVPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHLTGINVATCHDLNTYLHLVDGFVSS 425
           R   +WI+K VEETQ WAYSEVGRELRLSSRDSPHL  INVATCH LNTYLHLVDGFVSS
Sbjct: 361 R--LRWIQKYVEETQ-WAYSEVGRELRLSSRDSPHLNRINVATCHHLNTYLHLVDGFVSS 417

Query: 426 TCPFRATARRI 427
           TCPFRATARR+
Sbjct: 421 TCPFRATARRM 417

BLAST of Tan0020455 vs. TAIR 10
Match: AT2G44810.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 508.4 bits (1308), Expect = 5.4e-144
Identity = 262/362 (72.38%), Postives = 302/362 (83.43%), Query Frame = 0

Query: 71  DYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPSYATSLHPKASLLERS 130
           +YQGLQNW+GLLDPLDDNLR EILRYGQFV++AY++FDFDPSSP+Y T   P+++LLERS
Sbjct: 2   EYQGLQNWDGLLDPLDDNLRREILRYGQFVESAYQAFDFDPSSPTYGTCRFPRSTLLERS 61

Query: 131 GLPSTGYRVSKNLRATSGIRLPSWVHKAPS-VAANSSWIGYVAVCHDKHEISRLGRRDIV 190
           GLP++GYR++KNLRATSGI LP W+ KAPS +A  SSWIGYVAVC DK EISRLGRRD+V
Sbjct: 62  GLPNSGYRLTKNLRATSGINLPRWIEKAPSWMATQSSWIGYVAVCQDKEEISRLGRRDVV 121

Query: 191 IAFRGTATCLEWLENLRATLTKLPE---ESCCSGSDPMAMVESGFLSLYTSESEWCPSLR 250
           I+FRGTATCLEWLENLRATLT LP     +  +GS+   MVESGFLSLYTS      SLR
Sbjct: 122 ISFRGTATCLEWLENLRATLTHLPNGPTGANLNGSNSGPMVESGFLSLYTSGVH---SLR 181

Query: 251 QMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEYFKEKVGMVTVMSFG 310
            MVREEI+RLLQSYG+    PLS+TITGHSLGAA+ATLAAYDIK  FK +  MVTV+SFG
Sbjct: 182 DMVREEIARLLQSYGD---EPLSVTITGHSLGAAIATLAAYDIKTTFK-RAPMVTVISFG 241

Query: 311 GPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLK-EEAEGVRGGGRGVPQWIRKC 370
           GPRVGNR FRK +EKQGTKVLRIVNSDDVITKVPG VL+  E + V+     +P WI++ 
Sbjct: 242 GPRVGNRCFRKLLEKQGTKVLRIVNSDDVITKVPGVVLENREQDNVKMTASIMPSWIQRR 301

Query: 371 VEETQYWAYSEVGRELRLSSRDSPHLTGINVATCHDLNTYLHLVDGFVSSTCPFRATARR 428
           VEET  W Y+E+G+ELRLSSRDSPHL+ INVATCH+L TYLHLVDGFVSSTCPFR TARR
Sbjct: 302 VEETP-WVYAEIGKELRLSSRDSPHLSSINVATCHELKTYLHLVDGFVSSTCPFRETARR 355

BLAST of Tan0020455 vs. TAIR 10
Match: AT4G16820.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 343.6 bits (880), Expect = 2.3e-94
Identity = 190/374 (50.80%), Postives = 236/374 (63.10%), Query Frame = 0

Query: 62  KVKLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDP-SSPSYATSL 121
           K KLG KW++  GL NW GLLDPLD+NLR E++RYG+FV AAY +F  DP  SP +    
Sbjct: 124 KSKLGSKWRELHGLNNWAGLLDPLDENLRRELVRYGEFVQAAYHAFHSDPEGSPRHV--- 183

Query: 122 HPKASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHK-APS---VAANSSWIGYVAVCHD 181
                      LP   ++V+K+L ATS +RLP W+   AP    +   +SW+GYVAVC D
Sbjct: 184 ----------ALPDGSFKVTKSLYATSSVRLPKWIDDVAPDLRWMTKQTSWVGYVAVCDD 243

Query: 182 KHEISRLGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYT 241
             EI R+GRR+IVIA RGTAT LEW EN R  L  +PE            VE GF SLYT
Sbjct: 244 PREIRRMGRREIVIALRGTATLLEWSENFRPNLVSMPEPKPDQSDPTRPKVECGFNSLYT 303

Query: 242 SESEWCPSLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEYFKEK 301
           +  +  PSL + +  EISRL++ Y  GE+  LS+++TGHSLGAA+A LAA DI E     
Sbjct: 304 TGDQHAPSLAESLVGEISRLVELYA-GEE--LSISVTGHSLGAAIALLAADDIAERVPH- 363

Query: 302 VGMVTVMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLKEEAEG-VRGGG 361
              V V SFGGPRVGNR F  R++ +G KVLR+VNS DV+TKVPG     + +G  R  G
Sbjct: 364 APPVAVFSFGGPRVGNREFADRLDSKGVKVLRVVNSQDVVTKVPGIFADNDKQGQSRNNG 423

Query: 362 RGVPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHL-TGINVATCHDLNTYLHLVDGFVS 421
           R  P  I + VE    WAYS VG ELR+  + SP+L    +VA CHDL  YLHLVDGF++
Sbjct: 424 RS-PGGIMEMVERNNPWAYSHVGAELRVDMKMSPYLKPNADVACCHDLEAYLHLVDGFLA 479

Query: 422 STCPFRATARRILR 429
           S CPFRA A+R LR
Sbjct: 484 SNCPFRANAKRSLR 479

BLAST of Tan0020455 vs. TAIR 10
Match: AT2G30550.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 281.6 bits (719), Expect = 1.1e-75
Identity = 156/364 (42.86%), Postives = 218/364 (59.89%), Query Frame = 0

Query: 64  KLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPSYATSLHPK 123
           +L   W+  QG  +W GL+DP+D  LR+E++RYG+   A Y +FDFDP+S    TS   +
Sbjct: 100 RLRDTWRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTR 159

Query: 124 ASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHK---APSVAANSSWIGYVAVCHDKHEI 183
               +  G+  +GY V++ L ATS I LP++  K   +   + N++W+GYVAV  D+   
Sbjct: 160 LEFFDSLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSR 219

Query: 184 SRLGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYTSESE 243
           +RLGRRDI IA+RGT T LEW+ +L+  L  + E       DP   VESGFL LYT +  
Sbjct: 220 NRLGRRDIAIAWRGTVTKLEWIADLKDYLKPVTENK-IRCPDPAVKVESGFLDLYTDKDT 279

Query: 244 WCP----SLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEY---- 303
            C     S R+ +  E+ RL++ +G+ +   LS+T+TGHSLG ALA L+AYDI E     
Sbjct: 280 TCKFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNR 339

Query: 304 -FKEKVGMVTVMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLKEEAEGV 363
             K KV  VTV+++GGPRVGN  FR+R+E+ G KV+R+VN  DV+ K PG  L E     
Sbjct: 340 SKKGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESR--- 399

Query: 364 RGGGRGVPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHL-TGINVATCHDLNTYLHLVD 415
                  P  + K + E   W YS VG EL L  ++SP L   ++V+T H+L   LHL+D
Sbjct: 400 -------PHALMK-IAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLD 451

BLAST of Tan0020455 vs. TAIR 10
Match: AT2G30550.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 278.1 bits (710), Expect = 1.2e-74
Identity = 154/362 (42.54%), Postives = 216/362 (59.67%), Query Frame = 0

Query: 64  KLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPSYATSLHPK 123
           +L   W+  QG  +W GL+DP+D  LR+E++RYG+   A Y +FDFDP+S    TS   +
Sbjct: 100 RLRDTWRKIQGEDDWAGLMDPMDPILRSELIRYGEMAQACYDAFDFDPASKYCGTSRFTR 159

Query: 124 ASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHK---APSVAANSSWIGYVAVCHDKHEI 183
               +  G+  +GY V++ L ATS I LP++  K   +   + N++W+GYVAV  D+   
Sbjct: 160 LEFFDSLGMIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDETSR 219

Query: 184 SRLGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYTSESE 243
           +RLGRRDI IA+RGT T LEW+ +L+  L  + E       DP   VESGFL LYT +  
Sbjct: 220 NRLGRRDIAIAWRGTVTKLEWIADLKDYLKPVTENK-IRCPDPAVKVESGFLDLYTDKDT 279

Query: 244 WCP----SLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEY---- 303
            C     S R+ +  E+ RL++ +G+ +   LS+T+TGHSLG ALA L+AYDI E     
Sbjct: 280 TCKFARFSAREQILTEVKRLVEEHGDDDDSDLSITVTGHSLGGALAILSAYDIAEMRLNR 339

Query: 304 -FKEKVGMVTVMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLKEEAEGV 363
             K KV  VTV+++GGPRVGN  FR+R+E+ G KV+R+VN  DV+ K PG  L E     
Sbjct: 340 SKKGKVIPVTVLTYGGPRVGNVRFRERMEELGVKVMRVVNVHDVVPKSPGLFLNESR--- 399

Query: 364 RGGGRGVPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHL-TGINVATCHDLNTYLHLVD 413
                  P  + K + E   W YS VG EL L  ++SP L   ++V+T H+L   LHL+D
Sbjct: 400 -------PHALMK-IAEGLPWCYSHVGEELALDHQNSPFLKPSVDVSTAHNLEAMLHLLD 449

BLAST of Tan0020455 vs. TAIR 10
Match: AT1G06800.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 262.3 bits (669), Expect = 6.8e-70
Identity = 153/363 (42.15%), Postives = 208/363 (57.30%), Query Frame = 0

Query: 64  KLGKKWKDYQGLQNWEGLLDPLDDNLRNEILRYGQFVDAAYKSFDFDPSSPSYATSLHPK 123
           +L   W+  QG  +W GL+DP+D  LR+E++RYG+   A Y +FDFDP S    +    +
Sbjct: 84  RLRDTWRKIQGEDDWAGLMDPMDPVLRSELIRYGEMAQACYDAFDFDPFSRYCGSCRFTR 143

Query: 124 ASLLERSGLPSTGYRVSKNLRATSGIRLPSWVHK---APSVAANSSWIGYVAVCHDKHEI 183
             L +  G+  +GY V++ L ATS I LP++  K   +   + N++W+GYVAV  D    
Sbjct: 144 RHLFDSLGIIDSGYEVARYLYATSNINLPNFFSKSRWSKVWSKNANWMGYVAVSDDNEAT 203

Query: 184 -SRLGRRDIVIAFRGTATCLEWLENLRATLTKLPEESCCSGSDPMAMVESGFLSLYTSES 243
             RLGRRDI IA+RGT T LEW+ +L+  L K    +     DP    ESGFL LYT + 
Sbjct: 204 RCRLGRRDIAIAWRGTVTRLEWIADLKDFL-KPVSGNGFRCPDPAVKAESGFLDLYTDKD 263

Query: 244 EWCP----SLRQMVREEISRLLQSYGEGEQPPLSLTITGHSLGAALATLAAYDIKEY--- 303
             C     S R+ V  E+ RL++ YG+ E   LS+T+TGHSLG ALA L+AYD+ E    
Sbjct: 264 TSCNFSKFSAREQVLTEVKRLVERYGDEEGEELSITVTGHSLGGALAVLSAYDVAEMGVN 323

Query: 304 --FKEKVGMVTVMSFGGPRVGNRSFRKRVEKQGTKVLRIVNSDDVITKVPGFVLKEEAEG 363
              K KV  VT  ++GGPRVGN  F++R+EK G KVLR+VN  DV+ K PG  L E A  
Sbjct: 324 RTRKGKVIPVTAFTYGGPRVGNIRFKERIEKLGVKVLRVVNEHDVVAKSPGLFLNERA-- 383

Query: 364 VRGGGRGVPQWIRKCVEETQYWAYSEVGRELRLSSRDSPHL-TGINVATCHDLNTYLHLV 413
                   PQ + K       W YS VG  L L  + SP L   ++++T H+L   LHL+
Sbjct: 384 --------PQALMKLAGGLP-WCYSHVGEMLPLDHQKSPFLKPTVDLSTAHNLEALLHLL 434