Tan0020441 (gene) Snake gourd v1

BLAST of Tan0020441 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 4.9e-155
Identity = 273/516 (52.91%), Postives = 367/516 (71.12%), Query Frame = 0

Query: 27  ADKHQNFLHCLSEHSSDSYSISKVVYTPINSSYSSVLDFSIRNLRFSTADTPKPLVIITP 86
           A+  +NFL C S++   + + +K+VYT  +  Y S+L+ +I+NLRF++  TPKPLVIITP
Sbjct: 28  ANPQENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITP 87

Query: 87  LYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVSHLPFIIVDLINLKSIEVDVKKSTA 146
           L VSHIQ  I+CSK  GLQIRTRSGGHD EG+SY+S +PF+IVDL N+ S+++DV   TA
Sbjct: 88  LNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTA 147

Query: 147 WVQSGATLGELYYTIAEKSRTLAFPAGVCPTVGVGGHFSGGGYGLMLRKYGLAADNVIDA 206
           WV++GATLGE+YY I E +  L+FPAG CPTVG GGHFSGGGYG ++R YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 207 TLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVQVPATVTICSTSRKLE-E 266
            LV+ +GK+ DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI S  + +E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIH 267

Query: 267 DAIKLIHHWQYVANKLDENLFLGIILIGGNISTQEGITNPT--ATFFSLFLGKVDELVAI 326
           + +KL++ WQ +A   ++ L L    I  NI+  +G    T  + F S+F G VD LV +
Sbjct: 268 ELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDL 327

Query: 327 LKTNFPELGLIKEDCVETSWIESTLIAATGV----QTLESLEPLLNRTPPTNESTKIKSD 386
           +  +FPELG+ K DC + SWI+ T+I  +GV     T    E LL+R+     +  IK D
Sbjct: 328 MNKSFPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTYFKKEILLDRSGGRKAAFSIKLD 387

Query: 387 YVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKMSQISESETPFSHRAGNLYKIGYV 446
           YVK+PIPE A+  I ++L  +D+ V   +F PYGG M +ISES  PF HRAG +Y+I Y+
Sbjct: 388 YVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYI 447

Query: 447 VAWKEQSVDAKKRHLSWIRELYDYMAPFVSNSPRAAYANYRDLDIGTNNKYGKTSYKRAR 506
            +W++Q  +  ++H++WIR +Y++  P+VS +PR AY NYRDLD+G  N     +Y +AR
Sbjct: 448 ASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQAR 507

Query: 507 IWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTL 536
           IWG KYFG NFNRLV VKTKVDP +FFR+EQSIP L
Sbjct: 508 IWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Tan0020441 vs. ExPASy Swiss-Prot
Match: Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)

HSP 1 Score: 548.5 bits (1412), Expect = 8.4e-155
Identity = 273/540 (50.56%), Postives = 377/540 (69.81%), Query Frame = 0

Query: 1   MKYSSLIPLSLVFIVLS------PSPLWVAASADKHQNFLHCLSEHSSDSYSISKVVYTP 60
           MK+ S     L+F   S      P P  V+A+     +FL CLS   +DS  +SKV++TP
Sbjct: 1   MKFQSFFSSVLIFFTTSTLLLSIPHP--VSANRSNQSSFLQCLSLQLNDSNIVSKVIHTP 60

Query: 61  INSSYSSVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHD 120
            ++S+SSVL  SI+N RFS  D PKP++I+TP+  S +Q+A+ C++  G+ IRTRSGGHD
Sbjct: 61  NDTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHD 120

Query: 121 FEGLSYVSHLPFIIVDLINLKSIEVDVKKSTAWVQSGATLGELYYTIAEKSRTLAFPAGV 180
           +EGLSYV+H PF+I+DL NL+SI VDV   + WVQ+GAT+GELYY I +K+RTLAFPAGV
Sbjct: 121 YEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGV 180

Query: 181 CPTVGVGGHFSGGGYGLMLRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYG +LRK+GLAAD+VIDA +VDA G+I +R  MGED FWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVQVPATVTICSTSRKLEEDAIKLIHHWQYVANKLDENLFLGIILIGG 300
            SF +V++WK+ L+ VP+TVT+ + ++  E+ A+K+IH WQ+VA+K+ ++LF+ ++L   
Sbjct: 241 SSFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML--- 300

Query: 301 NISTQEGITNPTATFFSLFLGKVDELVAILKTNFPELGLIKEDCVETSWIESTL-IAATG 360
               Q       A+F  L+LG V  L+ ++   FPELGL ++DC E SWIES +  A  G
Sbjct: 301 ----QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELG 360

Query: 361 VQTLESLEPLLNRTPPTNESTKIKSDYVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYG 420
            + +     +L +    + + K KSD+V+EP+P+ AI  +W+RL+  + E  QLIF P+G
Sbjct: 361 EEPIN----VLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFG 420

Query: 421 GKMSQISESETPFSHRAGNLYKIGYVVAWKEQSVDAKKRHLSWIRELYDYMAPFVSNSPR 480
           GKMS+I++ ETPF HR GN+Y+I Y+  W+    D K++++ W+  +YD M+ FV+ SPR
Sbjct: 421 GKMSEIADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPR 480

Query: 481 AAYANYRDLDIGTNNKYGKTSYKRARIWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIP 534
            AY N RDLD+G      ++ Y+  + WG+KYF NNF RLV VKT VDP DFF  EQSIP
Sbjct: 481 GAYINLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Tan0020441 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 1.1e-154
Identity = 272/516 (52.71%), Postives = 366/516 (70.93%), Query Frame = 0

Query: 27  ADKHQNFLHCLSEHSSDSYSISKVVYTPINSSYSSVLDFSIRNLRFSTADTPKPLVIITP 86
           A+  +NFL CLS++   + + +K+VYT  +  Y S+L+ +++NLRF++  TPKPLVI TP
Sbjct: 28  ANPQENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTP 87

Query: 87  LYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVSHLPFIIVDLINLKSIEVDVKKSTA 146
           L VSHIQ  I+CSK  GLQIRTRSGGHD EG+SY+S +PF+IVDL N+ S+++DV   TA
Sbjct: 88  LNVSHIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTA 147

Query: 147 WVQSGATLGELYYTIAEKSRTLAFPAGVCPTVGVGGHFSGGGYGLMLRKYGLAADNVIDA 206
           WV+SGATLGE+YY I E +  L+FPAG CPTVG GGHFSGGGYG ++R YGLAADN+IDA
Sbjct: 148 WVESGATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 207 TLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVQVPATVTICSTSRKLE-E 266
            LV+ +GK+ DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI S  + +E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIH 267

Query: 267 DAIKLIHHWQYVANKLDENLFLGIILIGGNISTQEGITNPT--ATFFSLFLGKVDELVAI 326
           + +KL++ WQ +A   ++ L L    I  NI+  +G    T  + F S+F G VD LV +
Sbjct: 268 ELVKLVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDL 327

Query: 327 LKTNFPELGLIKEDCVETSWIESTLIAATGVQTLESL----EPLLNRTPPTNESTKIKSD 386
           +  +FPELG+ K DC + SWI+ T+I  +GV    +     E LL+R+     +  IK D
Sbjct: 328 MNKSFPELGIKKTDCKQLSWID-TIIFYSGVVNYNTTNFKKEILLDRSGGRKAAFSIKLD 387

Query: 387 YVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKMSQISESETPFSHRAGNLYKIGYV 446
           YVK+PIPE A+  I ++L  +D+ V   +F PYGG M +ISES  PF HRAG  Y+I Y+
Sbjct: 388 YVKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYI 447

Query: 447 VAWKEQSVDAKKRHLSWIRELYDYMAPFVSNSPRAAYANYRDLDIGTNNKYGKTSYKRAR 506
            +W++Q  +  ++H++WIR +Y++  P+VS +PR AY NYRDLD+G  N     +Y +AR
Sbjct: 448 ASWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQAR 507

Query: 507 IWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTL 536
           IWG KYFG NFNRLV VKTKVDP +FFR+EQSIP L
Sbjct: 508 IWGEKYFGKNFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Tan0020441 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 547.4 bits (1409), Expect = 1.9e-154
Identity = 273/518 (52.70%), Postives = 362/518 (69.88%), Query Frame = 0

Query: 27  ADKHQNFLHCLSEHSSDSYSISKVVYTPINSSYSSVLDFSIRNLRFSTADTPKPLVIITP 86
           A+  +NFL C S+H  ++ +  K+VYT  +  Y S+L+ +I+NLRF +  TPKPLVI+TP
Sbjct: 28  ANPRENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTP 87

Query: 87  LYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVSHLPFIIVDLINLKSIEVDVKKSTA 146
              SHIQA I+CSK  GLQIRTRSGGHD EG+SY+S +PF++VDL N+ SI++DV   TA
Sbjct: 88  SNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTA 147

Query: 147 WVQSGATLGELYYTIAEKSRTLAFPAGVCPTVGVGGHFSGGGYGLMLRKYGLAADNVIDA 206
           WV++GATLGE+YY I EK+  L+FP G CPTVGVGGHFSGGGYG ++R YGLAADN+IDA
Sbjct: 148 WVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDA 207

Query: 207 TLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVQVPATVTICSTSRKLE-E 266
            LV+ +GK+ DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  TI S  + +E  
Sbjct: 208 HLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIH 267

Query: 267 DAIKLIHHWQYVANKLDENLFLGIILIGGNISTQEGITNPT--ATFFSLFLGKVDELVAI 326
             +KL + WQ +A K D++L L    I  NI+   G    T    F S+F G VD LV +
Sbjct: 268 GLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDL 327

Query: 327 LKTNFPELGLIKEDCVETSWIESTLIAATGVQTLESL----EPLLNRTPPTNESTKIKSD 386
           +  +FPELG+ K DC E SWI++T I  +GV    +     E LL+R+     +  IK D
Sbjct: 328 MNKSFPELGIKKTDCKEFSWIDTT-IFYSGVVNFNTANFKKEILLDRSAGKKTAFSIKLD 387

Query: 387 YVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKMSQISESETPFSHRAGNLYKIGYV 446
           YVK+PIPE A+  I ++L  +D+     +  PYGG M +ISES  PF HRAG +Y++ Y 
Sbjct: 388 YVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYT 447

Query: 447 VAWKEQSVDAKKRHLSWIRELYDYMAPFVSNSPRAAYANYRDLDIGTNNKYGKTSYKRAR 506
            +W++Q  +  ++H++W+R +Y++  P+VS +PR AY NYRDLD+G  N     +Y +AR
Sbjct: 448 ASWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQAR 507

Query: 507 IWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTLSP 538
           IWG KYFG NFNRLV VKTKVDP +FFR+EQSIP L P
Sbjct: 508 IWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPLPP 542

BLAST of Tan0020441 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 1.7e-152
Identity = 272/540 (50.37%), Postives = 382/540 (70.74%), Query Frame = 0

Query: 1   MKYSSLIPLSLVFIVLSPSPLWVAASADKHQNFLHCLSEHSSDSYSISKVVYTPINSSYS 60
           M++SS +  +++   L+ SPL V+A    H++FL CLS   +D+    KV++T  +SS+ 
Sbjct: 1   MEFSSFL-FTILLFSLNISPL-VSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFF 60

Query: 61  SVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSY 120
           S+LD SI+N RFS ++TPKP+ IITP+  S +Q  I C++ HG+ +RTRS GH +EGLSY
Sbjct: 61  SILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSY 120

Query: 121 VSH-LPFIIVDLINLKSIEVDVKKSTAWVQSGATLGELYYTIAEKSRTLAFPAGVCPTVG 180
           +++  PF ++DL NL+SI +DV   T WVQ+GAT GELYY I + +++LAFPAG+ PTVG
Sbjct: 121 IAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVG 180

Query: 181 VGGHFSGGGYGLMLRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGI 240
           VGG FSGGGYG +LRKYGLAADN+IDA +VDA+G+I DR++MGED FWAIRGGGG SFG+
Sbjct: 181 VGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGV 240

Query: 241 VVAWKVKLVQVPATVTICSTSRKLEEDAIKLIHHWQYVANKLDENLFLGIILIGGNISTQ 300
           +++WKVKLV VP+T+T+    +  +++A+++I  WQY A+K+ ++LF+   L   N +  
Sbjct: 241 ILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSNKNAV 300

Query: 301 EGITNPTATFFSLFLGKVDELVAILKTNFPELGLIKEDCVETSWIESTLIAATGVQTLES 360
                  A F  L++G V+ L+A+++  FPELGL KE C E SWIES L  A   +  ES
Sbjct: 301 H------ALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKG-ES 360

Query: 361 LEPLLNRTPPTNESTKIKSDYVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKMSQI 420
           L  L NR   T+ S K K D+V+EPIPEAAI+ IW+RL+A +  + ++I  P+GGKMS++
Sbjct: 361 LGVLTNR-ERTSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEM 420

Query: 421 SESETPFSHRAGNLYKIGYVVAWKEQSVDAK---KRHLSWIRELYDYMAPFVSNSPRAAY 480
           +E ETPF HR GNLY+I YV  W+E+    K    ++L W+  +Y++M P+VS SPR AY
Sbjct: 421 AEYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAY 480

Query: 481 ANYRDLDIGTNNKYGKTSYKRARIWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTLS 537
            N++D+D+G      KT Y+  + WG+KYF NNF RLV VKT+VDP DFF  EQSIP ++
Sbjct: 481 VNFKDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPLVN 530

BLAST of Tan0020441 vs. NCBI nr
Match: XP_038895543.1 (berberine bridge enzyme-like 18 [Benincasa hispida])

HSP 1 Score: 914.1 bits (2361), Expect = 5.8e-262
Identity = 445/536 (83.02%), Postives = 488/536 (91.04%), Query Frame = 0

Query: 1   MKYSS-LIPLSLVFIVLSPSPLWVAASADKHQNFLHCLSEHSSDSYSISKVVYTPINSSY 60
           MK+SS LIPL+LV IVL   PLW AAS  K+Q+FL CLSEHSS+SY ISKVVY+PINSSY
Sbjct: 1   MKFSSPLIPLALVLIVLYSCPLWAAASPQKYQHFLQCLSEHSSNSYPISKVVYSPINSSY 60

Query: 61  SSVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLS 120
           SSVLDFSI+NLRFS   TPKPL+IITP +VSHIQAA+ICSK+HGLQIRTRSGGHDFEGLS
Sbjct: 61  SSVLDFSIKNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120

Query: 121 YVSHLPFIIVDLINLKSIEVDVKKSTAWVQSGATLGELYYTIAEKSRTLAFPAGVCPTVG 180
           YV++ PFI+VDLINL+SI VD + +TAWV+SGATLGELYY I EKSRTLAFPAGVCPTVG
Sbjct: 121 YVAYRPFIVVDLINLRSISVDDENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVG 180

Query: 181 VGGHFSGGGYGLMLRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGI 240
           +GGHFSGGGYGLMLRK+GLAADNVIDA LVDANGK+HDRESMGEDLFWAIRGGGGGSFGI
Sbjct: 181 IGGHFSGGGYGLMLRKFGLAADNVIDAHLVDANGKVHDRESMGEDLFWAIRGGGGGSFGI 240

Query: 241 VVAWKVKLVQVPATVTICSTSRKLEEDAIKLIHHWQYVANKLDENLFLGIILIGGNISTQ 300
           VVAWK+KLV VPATVTICST R LEEDAIKL+H WQYVANKLDENL+LGIIL GG  STQ
Sbjct: 241 VVAWKIKLVPVPATVTICSTQRNLEEDAIKLVHRWQYVANKLDENLYLGIILTGGKASTQ 300

Query: 301 EGITNPTATFFSLFLGKVDELVAILKTNFPELGLIKEDCVETSWIESTLIAATGVQTLES 360
            GITNPTATFFSLFLG++DEL+A L T FPELGLIK+DCVE +W+ESTLI ATGVQ +ES
Sbjct: 301 GGITNPTATFFSLFLGRIDELLATLSTTFPELGLIKQDCVEATWVESTLIIATGVQPIES 360

Query: 361 LEPLLNRTPPTNESTKIKSDYVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKMSQI 420
           LEPLLNRTPPT E+TKIKSDYVKEP+PEAAIEGI QRLKAQDIE  QLIFVPYGGKMSQI
Sbjct: 361 LEPLLNRTPPTIENTKIKSDYVKEPLPEAAIEGICQRLKAQDIETSQLIFVPYGGKMSQI 420

Query: 421 SESETPFSHRAGNLYKIGYVVAWKEQSVDAKKRHLSWIRELYDYMAPFVSNSPRAAYANY 480
           SESETPFSHRAGNLYKIGYVV WKEQS+ AKKRH+ WIRE+Y+YM PFVS SPRAAYANY
Sbjct: 421 SESETPFSHRAGNLYKIGYVVGWKEQSLKAKKRHIHWIREVYEYMIPFVSKSPRAAYANY 480

Query: 481 RDLDIGTNNKYGKTSYKRARIWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTL 536
           RDLDIG NNKYG+TS ++A +WGLKYFGNNF +LV+VKTKVDPYDFFRHEQSIPTL
Sbjct: 481 RDLDIGANNKYGQTSVEQASVWGLKYFGNNFKKLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Tan0020441 vs. NCBI nr
Match: XP_038895508.1 (berberine bridge enzyme-like 28 [Benincasa hispida])

HSP 1 Score: 907.1 bits (2343), Expect = 7.1e-260
Identity = 447/537 (83.24%), Postives = 486/537 (90.50%), Query Frame = 0

Query: 1   MKYSS-LIPLSLVFIVLSPSPLWVAASADKHQNFLHCLSEHSSDSYSISKVVYTPINSSY 60
           MKY+S LI LSLV IV+S SPLW +AS  K+Q+FL CL EHS D+Y ISKVVY+PINSSY
Sbjct: 1   MKYTSPLILLSLVLIVVSSSPLWASASEQKYQHFLQCLLEHSLDNYPISKVVYSPINSSY 60

Query: 61  SSVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLS 120
           SSVLDFSIRNLRFS   TPKPL+IITP +VSHIQAA+ICSK+HGLQIRTRSGGHDFEGLS
Sbjct: 61  SSVLDFSIRNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120

Query: 121 YVSHLPFIIVDLINLKSIEVDVKKSTAWVQSGATLGELYYTIAEKSRTLAFPAGVCPTVG 180
           YV++LPFIIVDLINL+S+ +DV+ STAWVQSGATLGELYY I EKSRTLAFPAGVCPTVG
Sbjct: 121 YVAYLPFIIVDLINLRSVTIDVENSTAWVQSGATLGELYYRIGEKSRTLAFPAGVCPTVG 180

Query: 181 VGGHFSGGGYGLMLRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGI 240
           VGGHFSGGGYG MLRK+GLAADNVIDA LVDANGK+HDRESMGEDLFWAIRGGGGGSFGI
Sbjct: 181 VGGHFSGGGYGFMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGI 240

Query: 241 VVAWKVKLVQVPATVTICSTSRKLEEDAIKLIHHWQYVANKLDENLFLGIILIGGNI-ST 300
           VVAWKVKLV VPATVT CST R LE DAIKL+H WQYVANKLDENLFLGI+L GGNI +T
Sbjct: 241 VVAWKVKLVPVPATVTSCSTHRNLEGDAIKLVHQWQYVANKLDENLFLGILLTGGNITTT 300

Query: 301 QEGITNPTATFFSLFLGKVDELVAILKTNFPELGLIKEDCVETSWIESTLIAATGVQTLE 360
           QEGI +PTATFF LFLGKVDELVA L T FPEL L+K+DCVETSWIESTLIAATGVQ +E
Sbjct: 301 QEGIADPTATFFILFLGKVDELVATLSTTFPELSLMKQDCVETSWIESTLIAATGVQIVE 360

Query: 361 SLEPLLNRTPPTNESTKIKSDYVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKMSQ 420
           SLEPLLNRTPPT ESTKIKSDY+KEPIPEA IEGI+QRLKAQDI   QLIFVPYGG+MSQ
Sbjct: 361 SLEPLLNRTPPTIESTKIKSDYIKEPIPEATIEGIFQRLKAQDIATSQLIFVPYGGRMSQ 420

Query: 421 ISESETPFSHRAGNLYKIGYVVAWKEQSVDAKKRHLSWIRELYDYMAPFVSNSPRAAYAN 480
           ISESETPFSHRA NLYKIGY+V WKEQS++AKKRH+SWIRE+Y+YM PFVS SPR+AYAN
Sbjct: 421 ISESETPFSHRAANLYKIGYIVGWKEQSLNAKKRHISWIREVYEYMTPFVSKSPRSAYAN 480

Query: 481 YRDLDIGTNNKYGKTSYKRARIWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTL 536
           YRDLDIG NNKY KTS K+A +WGLKYFGNNF RLV+VKTKVDPYDFFRHEQSIP L
Sbjct: 481 YRDLDIGANNKYRKTSVKQASVWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPAL 537

BLAST of Tan0020441 vs. NCBI nr
Match: XP_004151192.3 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653316.1 hypothetical protein Csa_023212 [Cucumis sativus])

HSP 1 Score: 899.0 bits (2322), Expect = 1.9e-257
Identity = 437/537 (81.38%), Postives = 485/537 (90.32%), Query Frame = 0

Query: 1   MKYSS-LIPLSLVFIVLSPSPLWVAASADKHQNFLHCLSEHSSDSYSISKVVYTPINSSY 60
           MKYSS LIP SL+ IV+S S LWV+AS  KH NFL CLSEHSS SY ISKVV+TPINSSY
Sbjct: 1   MKYSSPLIPFSLILIVVSSSSLWVSASPHKHHNFLQCLSEHSSKSYPISKVVHTPINSSY 60

Query: 61  SSVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLS 120
           SSVL+FSIRNLRFS  +TPKPL+IITP +VSHIQAA+ICSK+HGLQIRTRSGGHD+EGLS
Sbjct: 61  SSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEGLS 120

Query: 121 YVSHLPFIIVDLINLKSIEVDVKKSTAWVQSGATLGELYYTIAEKSRTLAFPAGVCPTVG 180
           YV+  PFII+DLINL+S+++DV+ +TAWV+SG TLGELYY I EKSRTLAFPAG+CPTVG
Sbjct: 121 YVASHPFIILDLINLRSMKIDVESNTAWVESGTTLGELYYRIGEKSRTLAFPAGICPTVG 180

Query: 181 VGGHFSGGGYGLMLRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGI 240
           VGGHFSGGGYGLMLRK+GLAADNVIDA LVDANGK+HDRESMGEDLFWAIRGGGGGSFGI
Sbjct: 181 VGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGI 240

Query: 241 VVAWKVKLVQVPATVTICSTSRKLEEDAIKLIHHWQYVANKLDENLFLGIILIGGNI-ST 300
           VVAWK+KLV VPATVT+C+T R L+ D IKL+H WQYVANKLDENLFLGIIL GGNI +T
Sbjct: 241 VVAWKIKLVSVPATVTMCNTYRNLKGDGIKLVHQWQYVANKLDENLFLGIILTGGNITTT 300

Query: 301 QEGITNPTATFFSLFLGKVDELVAILKTNFPELGLIKEDCVETSWIESTLIAATGVQTLE 360
           QEGITNP ATFFSLFLGKV+ELVA L T FPELGLIK+DC+ETSWIESTLIA+TGVQT+E
Sbjct: 301 QEGITNPIATFFSLFLGKVNELVATLSTTFPELGLIKQDCIETSWIESTLIASTGVQTVE 360

Query: 361 SLEPLLNRTPPTNESTKIKSDYVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKMSQ 420
           SLEPLLNRTP   E+ KIKSDY+KEPI  A IEGIWQRLKAQDIE  QLIF+PYGG+MSQ
Sbjct: 361 SLEPLLNRTPSNLENEKIKSDYIKEPISIATIEGIWQRLKAQDIETSQLIFIPYGGRMSQ 420

Query: 421 ISESETPFSHRAGNLYKIGYVVAWKEQSVDAKKRHLSWIRELYDYMAPFVSNSPRAAYAN 480
           ISESETPFSHR GNLYKIGY++ WKEQS+ AKK+H+SWIRE+Y+YM PFVS SPR+AYAN
Sbjct: 421 ISESETPFSHRVGNLYKIGYILGWKEQSLKAKKKHISWIREIYEYMTPFVSKSPRSAYAN 480

Query: 481 YRDLDIGTNNKYGKTSYKRARIWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTL 536
           YRDLDIG N KYGKTS K+A IWGLKYFGNNF RLV+VKTKVDPYDFFRHEQSIPTL
Sbjct: 481 YRDLDIGVNKKYGKTSVKQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of Tan0020441 vs. NCBI nr
Match: KAG6583829.1 (Berberine bridge enzyme-like 26, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 897.5 bits (2318), Expect = 5.6e-257
Identity = 430/528 (81.44%), Postives = 483/528 (91.48%), Query Frame = 0

Query: 7   IPLSLVF-IVLSPSPLWVAASADKHQNFLHCLSEHSSDSYSISKVVYTPINSSYSSVLDF 66
           IPL+L+F IVLS SPLW + S   HQ+FLHCLS HS  +Y ISK+VYTPINSSYS VL+F
Sbjct: 4   IPLTLIFIIVLSSSPLWASPSLHIHQHFLHCLSHHSPHTYPISKLVYTPINSSYSYVLNF 63

Query: 67  SIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVSHLP 126
           SIRNL FS   TPKPL+IITP ++SHIQAA+ICSK H LQIRTRSGGHDFEGLSYV+  P
Sbjct: 64  SIRNLIFSNPQTPKPLLIITPSHISHIQAAVICSKAHDLQIRTRSGGHDFEGLSYVADQP 123

Query: 127 FIIVDLINLKSIEVDVKKSTAWVQSGATLGELYYTIAEKSRTLAFPAGVCPTVGVGGHFS 186
           FIIVDLINL+SI VDV+ +TAWVQSGATLGELYY I EKSR+LAFPAG+CPTVGVGGHFS
Sbjct: 124 FIIVDLINLRSINVDVQTNTAWVQSGATLGELYYRIGEKSRSLAFPAGICPTVGVGGHFS 183

Query: 187 GGGYGLMLRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 246
           GGGYGLMLRKYGLAADNV+DA LVDANG++HDRESMGEDLFWAIRGGGGGSFGIVVAWKV
Sbjct: 184 GGGYGLMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 243

Query: 247 KLVQVPATVTICSTSRKLEEDAIKLIHHWQYVANKLDENLFLGIILIGGNISTQEGITNP 306
           KLV VPA VT+CST+R +E+DAIKLIH WQYVAN+LD++LFLGI+L GGN ST+EGITNP
Sbjct: 244 KLVVVPAVVTMCSTNRDVEDDAIKLIHRWQYVANQLDDDLFLGILLTGGNFSTREGITNP 303

Query: 307 TATFFSLFLGKVDELVAILKTNFPELGLIKEDCVETSWIESTLIAATGVQTLESLEPLLN 366
           TATFFSL+LGK+DEL+AILKT FPELGL K+DCVE+SWI+STLIA+TGVQT E LE LLN
Sbjct: 304 TATFFSLYLGKLDELIAILKTTFPELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALLN 363

Query: 367 RTPPTNESTKIKSDYVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKMSQISESETP 426
           RTPPT ESTK KSDYVK+PIPEAAIEGIWQRLKAQDIEVPQ++F+PYGG+MSQISESETP
Sbjct: 364 RTPPTIESTKFKSDYVKDPIPEAAIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESETP 423

Query: 427 FSHRAGNLYKIGYVVAWKEQSVDAKKRHLSWIRELYDYMAPFVSNSPRAAYANYRDLDIG 486
           FSHRAGNLYKIGY V+WKEQS+DAK RH++WIRE+Y+YMAPFVS SPRAAY NYRDLDIG
Sbjct: 424 FSHRAGNLYKIGYAVSWKEQSLDAKNRHINWIREVYEYMAPFVSKSPRAAYVNYRDLDIG 483

Query: 487 TNNKYGKTSYKRARIWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIP 534
           TNNKYGKTSY++A +WG+KYFGNNFNRLV+VKT VDP+DFFRHEQSIP
Sbjct: 484 TNNKYGKTSYEQASVWGMKYFGNNFNRLVYVKTMVDPHDFFRHEQSIP 531

BLAST of Tan0020441 vs. NCBI nr
Match: XP_023001021.1 (berberine bridge enzyme-like 18 [Cucurbita maxima])

HSP 1 Score: 897.5 bits (2318), Expect = 5.6e-257
Identity = 428/528 (81.06%), Postives = 482/528 (91.29%), Query Frame = 0

Query: 7   IPLSLVF-IVLSPSPLWVAASADKHQNFLHCLSEHSSDSYSISKVVYTPINSSYSSVLDF 66
           IPL+L+F IVLS SPLW + S  KHQ+FLHCLS HS  +Y ISK+VYTPINSSYS VL+F
Sbjct: 4   IPLTLIFIIVLSSSPLWASPSLHKHQHFLHCLSHHSPHTYPISKLVYTPINSSYSPVLNF 63

Query: 67  SIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVSHLP 126
           SIRNL FS   TPKPL+IITP ++SHIQAAI+CSK H LQIRTRSGGHDFEGLSYV++ P
Sbjct: 64  SIRNLIFSNPQTPKPLLIITPSHISHIQAAIVCSKAHDLQIRTRSGGHDFEGLSYVANQP 123

Query: 127 FIIVDLINLKSIEVDVKKSTAWVQSGATLGELYYTIAEKSRTLAFPAGVCPTVGVGGHFS 186
           FIIVDLINL+SI VDV+ +TAWVQSGATLGELYY I E SR+LAFPAG+CPTVGVGGHFS
Sbjct: 124 FIIVDLINLRSINVDVQTNTAWVQSGATLGELYYRIGETSRSLAFPAGICPTVGVGGHFS 183

Query: 187 GGGYGLMLRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 246
           GGGYGLMLRKYGLAADNV+DA LVDANG++HDRESMGEDLFWAIRGGGGGSFGIVVAWKV
Sbjct: 184 GGGYGLMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 243

Query: 247 KLVQVPATVTICSTSRKLEEDAIKLIHHWQYVANKLDENLFLGIILIGGNISTQEGITNP 306
           KLV VPA VT+CST+R +E+DAIKLIH WQYVAN+LD+NLFLGI+L GGN ST+EGITNP
Sbjct: 244 KLVMVPAVVTMCSTNRDVEDDAIKLIHRWQYVANQLDDNLFLGILLTGGNFSTREGITNP 303

Query: 307 TATFFSLFLGKVDELVAILKTNFPELGLIKEDCVETSWIESTLIAATGVQTLESLEPLLN 366
           TATFFSL+LGK+DEL+ ILK NF ELGL K+DCVE+SWI+STLIA+TGVQT E LE LLN
Sbjct: 304 TATFFSLYLGKLDELITILKNNFSELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALLN 363

Query: 367 RTPPTNESTKIKSDYVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKMSQISESETP 426
           RTPPT ESTK KSDYVK+PIPEA IEGIWQRLKAQDIEVPQ++F+PYGG+MSQISESETP
Sbjct: 364 RTPPTIESTKFKSDYVKDPIPEATIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESETP 423

Query: 427 FSHRAGNLYKIGYVVAWKEQSVDAKKRHLSWIRELYDYMAPFVSNSPRAAYANYRDLDIG 486
           FSHRAGNLYKIGY V+WKEQS+DAK +H++WIRE+Y+YM PFVS SPRAAYANYRDLDIG
Sbjct: 424 FSHRAGNLYKIGYAVSWKEQSLDAKNKHINWIREVYEYMTPFVSKSPRAAYANYRDLDIG 483

Query: 487 TNNKYGKTSYKRARIWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIP 534
           TNNKYGKTSY++A +WG+KYFGNNFNRLV+VKTKVDP+DFFRHEQSIP
Sbjct: 484 TNNKYGKTSYEQASVWGMKYFGNNFNRLVYVKTKVDPHDFFRHEQSIP 531

BLAST of Tan0020441 vs. ExPASy TrEMBL
Match: A0A6J1KHF6 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111495284 PE=3 SV=1)

HSP 1 Score: 897.5 bits (2318), Expect = 2.7e-257
Identity = 428/528 (81.06%), Postives = 482/528 (91.29%), Query Frame = 0

Query: 7   IPLSLVF-IVLSPSPLWVAASADKHQNFLHCLSEHSSDSYSISKVVYTPINSSYSSVLDF 66
           IPL+L+F IVLS SPLW + S  KHQ+FLHCLS HS  +Y ISK+VYTPINSSYS VL+F
Sbjct: 4   IPLTLIFIIVLSSSPLWASPSLHKHQHFLHCLSHHSPHTYPISKLVYTPINSSYSPVLNF 63

Query: 67  SIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVSHLP 126
           SIRNL FS   TPKPL+IITP ++SHIQAAI+CSK H LQIRTRSGGHDFEGLSYV++ P
Sbjct: 64  SIRNLIFSNPQTPKPLLIITPSHISHIQAAIVCSKAHDLQIRTRSGGHDFEGLSYVANQP 123

Query: 127 FIIVDLINLKSIEVDVKKSTAWVQSGATLGELYYTIAEKSRTLAFPAGVCPTVGVGGHFS 186
           FIIVDLINL+SI VDV+ +TAWVQSGATLGELYY I E SR+LAFPAG+CPTVGVGGHFS
Sbjct: 124 FIIVDLINLRSINVDVQTNTAWVQSGATLGELYYRIGETSRSLAFPAGICPTVGVGGHFS 183

Query: 187 GGGYGLMLRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 246
           GGGYGLMLRKYGLAADNV+DA LVDANG++HDRESMGEDLFWAIRGGGGGSFGIVVAWKV
Sbjct: 184 GGGYGLMLRKYGLAADNVVDAYLVDANGRVHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 243

Query: 247 KLVQVPATVTICSTSRKLEEDAIKLIHHWQYVANKLDENLFLGIILIGGNISTQEGITNP 306
           KLV VPA VT+CST+R +E+DAIKLIH WQYVAN+LD+NLFLGI+L GGN ST+EGITNP
Sbjct: 244 KLVMVPAVVTMCSTNRDVEDDAIKLIHRWQYVANQLDDNLFLGILLTGGNFSTREGITNP 303

Query: 307 TATFFSLFLGKVDELVAILKTNFPELGLIKEDCVETSWIESTLIAATGVQTLESLEPLLN 366
           TATFFSL+LGK+DEL+ ILK NF ELGL K+DCVE+SWI+STLIA+TGVQT E LE LLN
Sbjct: 304 TATFFSLYLGKLDELITILKNNFSELGLTKKDCVESSWIQSTLIASTGVQTFEPLEALLN 363

Query: 367 RTPPTNESTKIKSDYVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKMSQISESETP 426
           RTPPT ESTK KSDYVK+PIPEA IEGIWQRLKAQDIEVPQ++F+PYGG+MSQISESETP
Sbjct: 364 RTPPTIESTKFKSDYVKDPIPEATIEGIWQRLKAQDIEVPQILFIPYGGRMSQISESETP 423

Query: 427 FSHRAGNLYKIGYVVAWKEQSVDAKKRHLSWIRELYDYMAPFVSNSPRAAYANYRDLDIG 486
           FSHRAGNLYKIGY V+WKEQS+DAK +H++WIRE+Y+YM PFVS SPRAAYANYRDLDIG
Sbjct: 424 FSHRAGNLYKIGYAVSWKEQSLDAKNKHINWIREVYEYMTPFVSKSPRAAYANYRDLDIG 483

Query: 487 TNNKYGKTSYKRARIWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIP 534
           TNNKYGKTSY++A +WG+KYFGNNFNRLV+VKTKVDP+DFFRHEQSIP
Sbjct: 484 TNNKYGKTSYEQASVWGMKYFGNNFNRLVYVKTKVDPHDFFRHEQSIP 531

BLAST of Tan0020441 vs. ExPASy TrEMBL
Match: A0A5A7TU57 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00190 PE=3 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 8.2e-254
Identity = 432/536 (80.60%), Postives = 480/536 (89.55%), Query Frame = 0

Query: 1   MKYSS-LIPLSLVFIVLSPSPLWVAASADKHQNFLHCLSEHSSDSYSISKVVYTPINSSY 60
           MK+SS LIPLSLVFIV+S + L  +AS  K+QNFL CLSEHSS SY ISKVVYTPINSSY
Sbjct: 1   MKFSSTLIPLSLVFIVVSSASLCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSSY 60

Query: 61  SSVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLS 120
           SSVLDFSIRNL FS  +TPKPL+IITP +VSHIQAA+ICSK+HGLQIRTRSGGHDFEGLS
Sbjct: 61  SSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120

Query: 121 YVSHLPFIIVDLINLKSIEVDVKKSTAWVQSGATLGELYYTIAEKSRTLAFPAGVCPTVG 180
           YV++ PFI+VDLINL+S++V+V+ +TAWV+SGATLGELYY I EKSRTLAFPAGVCPTVG
Sbjct: 121 YVAYRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVG 180

Query: 181 VGGHFSGGGYGLMLRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGI 240
           +GGHFSGGGYGLMLRK+GLAADNVIDA LVDANGK+ D+ESMGEDLFWAIRGGGGGSFGI
Sbjct: 181 IGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGI 240

Query: 241 VVAWKVKLVQVPATVTICSTSRKLEEDAIKLIHHWQYVANKLDENLFLGIILIGGNISTQ 300
           VVAWK+KLV VP TVTICST R LEEDAIKLIH WQYV NKLDEN++LGIIL GGN STQ
Sbjct: 241 VVAWKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNASTQ 300

Query: 301 EGITNPTATFFSLFLGKVDELVAILKTNFPELGLIKEDCVETSWIESTLIAATGVQTLES 360
            GITNPTA FFSLFLG+ DELV  L T FPELGLIK+DCVE SW+ESTLI   GVQ +E+
Sbjct: 301 AGITNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIET 360

Query: 361 LEPLLNRTPPTNESTKIKSDYVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKMSQI 420
           LEPLLNRT  T +STKIKSDY+ EPIP AAI+ IWQRLK QDIE  QL+FVPYGG+MSQI
Sbjct: 361 LEPLLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQI 420

Query: 421 SESETPFSHRAGNLYKIGYVVAWKEQSVDAKKRHLSWIRELYDYMAPFVSNSPRAAYANY 480
           SESETPFSHRA  L+KIGYVV WK+QS+ AKKRH+SW+RE+Y+YMAPFVS SPRAAY NY
Sbjct: 421 SESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTNY 480

Query: 481 RDLDIGTNNKYGKTSYKRARIWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTL 536
           RDLDIG+NNKYGKTSYKRA +WGLKYFGNNF+RLV+VKTKVDPYDFFRHEQSIPTL
Sbjct: 481 RDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Tan0020441 vs. ExPASy TrEMBL
Match: A0A1S3BDA8 (tetrahydrocannabinolic acid synthase-like OS=Cucumis melo OX=3656 GN=LOC103488637 PE=3 SV=1)

HSP 1 Score: 885.9 bits (2288), Expect = 8.2e-254
Identity = 432/536 (80.60%), Postives = 480/536 (89.55%), Query Frame = 0

Query: 1   MKYSS-LIPLSLVFIVLSPSPLWVAASADKHQNFLHCLSEHSSDSYSISKVVYTPINSSY 60
           MK+SS LIPLSLVFIV+S + L  +AS  K+QNFL CLSEHSS SY ISKVVYTPINSSY
Sbjct: 1   MKFSSTLIPLSLVFIVVSSASLCASASPQKYQNFLQCLSEHSSKSYPISKVVYTPINSSY 60

Query: 61  SSVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLS 120
           SSVLDFSIRNL FS  +TPKPL+IITP +VSHIQAA+ICSK+HGLQIRTRSGGHDFEGLS
Sbjct: 61  SSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120

Query: 121 YVSHLPFIIVDLINLKSIEVDVKKSTAWVQSGATLGELYYTIAEKSRTLAFPAGVCPTVG 180
           YV++ PFI+VDLINL+S++V+V+ +TAWV+SGATLGELYY I EKSRTLAFPAGVCPTVG
Sbjct: 121 YVAYRPFIVVDLINLRSVKVEVENNTAWVESGATLGELYYRIGEKSRTLAFPAGVCPTVG 180

Query: 181 VGGHFSGGGYGLMLRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGI 240
           +GGHFSGGGYGLMLRK+GLAADNVIDA LVDANGK+ D+ESMGEDLFWAIRGGGGGSFGI
Sbjct: 181 IGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVLDKESMGEDLFWAIRGGGGGSFGI 240

Query: 241 VVAWKVKLVQVPATVTICSTSRKLEEDAIKLIHHWQYVANKLDENLFLGIILIGGNISTQ 300
           VVAWK+KLV VP TVTICST R LEEDAIKLIH WQYV NKLDEN++LGIIL GGN STQ
Sbjct: 241 VVAWKIKLVPVPETVTICSTDRNLEEDAIKLIHRWQYVGNKLDENIYLGIILTGGNASTQ 300

Query: 301 EGITNPTATFFSLFLGKVDELVAILKTNFPELGLIKEDCVETSWIESTLIAATGVQTLES 360
            GITNPTA FFSLFLG+ DELV  L T FPELGLIK+DCVE SW+ESTLI   GVQ +E+
Sbjct: 301 AGITNPTARFFSLFLGRADELVTTLSTIFPELGLIKQDCVEASWVESTLIIPIGVQPIET 360

Query: 361 LEPLLNRTPPTNESTKIKSDYVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKMSQI 420
           LEPLLNRT  T +STKIKSDY+ EPIP AAI+ IWQRLK QDIE  QL+FVPYGG+MSQI
Sbjct: 361 LEPLLNRTLTTLDSTKIKSDYITEPIPVAAIKDIWQRLKTQDIETSQLVFVPYGGRMSQI 420

Query: 421 SESETPFSHRAGNLYKIGYVVAWKEQSVDAKKRHLSWIRELYDYMAPFVSNSPRAAYANY 480
           SESETPFSHRA  L+KIGYVV WK+QS+ AKKRH+SW+RE+Y+YMAPFVS SPRAAY NY
Sbjct: 421 SESETPFSHRARYLFKIGYVVGWKDQSLKAKKRHISWLREVYEYMAPFVSKSPRAAYTNY 480

Query: 481 RDLDIGTNNKYGKTSYKRARIWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTL 536
           RDLDIG+NNKYGKTSYKRA +WGLKYFGNNF+RLV+VKTKVDPYDFFRHEQSIPTL
Sbjct: 481 RDLDIGSNNKYGKTSYKRASVWGLKYFGNNFDRLVYVKTKVDPYDFFRHEQSIPTL 536

BLAST of Tan0020441 vs. ExPASy TrEMBL
Match: A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.2e-252
Identity = 428/537 (79.70%), Postives = 477/537 (88.83%), Query Frame = 0

Query: 1   MKYSSLIPLSLVFIVLSPSPLWVAASADKHQNFLHCLSEHSSDSYSISKVVYTPINSSYS 60
           MKYS L  LSL FI+LS S  W AASA+KH+ FL CLS HS+DS SISKV+YTPIN SYS
Sbjct: 1   MKYSPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSSSISKVIYTPINPSYS 60

Query: 61  SVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSY 120
           SVL+FS+RNLRFS+A+TPKPL+IITP YVSH+QAA+ICSK HGLQIR RSGGHD EGLSY
Sbjct: 61  SVLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSY 120

Query: 121 VSHLPFIIVDLINLKSIEVDVKKSTAWVQSGATLGELYYTIAEKSRTLAFPAGVCPTVGV 180
           V+HLPFII+DLINLKSI V+++ STAW+QSGATLGELYY IA+KSRTLAFPAGVCPTVGV
Sbjct: 121 VAHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGV 180

Query: 181 GGHFSGGGYGLMLRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIV 240
           GGHFSGGGYGLMLRKYGLAADNVIDA L+DANGK +DRESMGEDLFWAIRGGGGGSFGIV
Sbjct: 181 GGHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIV 240

Query: 241 VAWKVKLVQVPATVTICSTSRKLEEDAIKLIHHWQYVANKLDENLFLGIILIGGNISTQE 300
           VAWKVKLV VPA VT CS+ R L+ED I LIH WQ VA KLDENL+ GIIL GGN ST+ 
Sbjct: 241 VAWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGNASTEG 300

Query: 301 GI--TNPTATFFSLFLGKVDELVAILKTNFPELGLIKEDCVETSWIESTLIAATGVQTLE 360
           G+   NPTATFFSLFLGK DEL+AIL TNFPEL L K+DCVETSWIESTL+AAT  QT+E
Sbjct: 301 GVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIE 360

Query: 361 SLEPLLNRTPPTNESTKIKSDYVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKMSQ 420
           SLE LLNRTP T E+TK+KSDYVKEPIP+A IEGIW+RLKAQDIEVPQL+FVPYGG+MS+
Sbjct: 361 SLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSR 420

Query: 421 ISESETPFSHRAGNLYKIGYVVAWKEQSVDAKKRHLSWIRELYDYMAPFVSNSPRAAYAN 480
           IS+SETPFSHRA NLYKIGY+V WKEQSV A++RHLSWIRE+YDYM PFVS SPRA Y N
Sbjct: 421 ISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVN 480

Query: 481 YRDLDIGTNNKYGKTSYKRARIWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTL 536
           YRDLDIGTNNKYGKTSYK+A +WG KYFGNNFNRLVH+K+KVDP +FF++EQSIP L
Sbjct: 481 YRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537

BLAST of Tan0020441 vs. ExPASy TrEMBL
Match: A0A5D3C1A1 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00110 PE=3 SV=1)

HSP 1 Score: 879.0 bits (2270), Expect = 1.0e-251
Identity = 433/537 (80.63%), Postives = 476/537 (88.64%), Query Frame = 0

Query: 1   MKYSS-LIPLSLVFIVLSPSPLWVAASADKHQNFLHCLSEHSSDSYSISKVVYTPINSSY 60
           MKYSS LIP SL+ I++S S  WV+AS  KHQNFL CLSEHSS  Y ISKVV+TPINSSY
Sbjct: 1   MKYSSPLIPFSLILILVSSSSSWVSASPHKHQNFLQCLSEHSSKRYPISKVVHTPINSSY 60

Query: 61  SSVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLS 120
           SSVLDFSIRNL FS  +TPKPL+IITP +VSHIQAA+ICSK+HGLQIRTRSGGHDFEGLS
Sbjct: 61  SSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLS 120

Query: 121 YVSHLPFIIVDLINLKSIEVDVKKSTAWVQSGATLGELYYTIAEKSRTLAFPAGVCPTVG 180
           YV++ PFIIVDLINL+SI++DV+ +TAWV+SGATLGELYY I EKSRTLAFP GVCPTVG
Sbjct: 121 YVAYHPFIIVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPTVG 180

Query: 181 VGGHFSGGGYGLMLRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGI 240
           VGGHFSGGGYGLMLRK+GLAADNVIDA LVDANGK+HDR+SMGEDLFWAIRGGGGGSFGI
Sbjct: 181 VGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSFGI 240

Query: 241 VVAWKVKLVQVPATVTICSTSRKLEEDAIKLIHHWQYVANKLDENLFLGIILIGGNI-ST 300
           VVAWK+KLV VPATVTICST+R L+ D IKL+H WQYVANKLDENLFLGI+L GGNI +T
Sbjct: 241 VVAWKIKLVSVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNITTT 300

Query: 301 QEGITNPTATFFSLFLGKVDELVAILKTNFPELGLIKEDCVETSWIESTLIAATGVQTLE 360
           QEGI NP ATFFSLFLGKVD LVA L   FPELG  KEDCVETSWIESTLIAATGV T+E
Sbjct: 301 QEGIANPIATFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVGTVE 360

Query: 361 SLEPLLNRTPPTNESTKIKSDYVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKMSQ 420
           SLEPLLNRTP   E+ K+KSDY KEPI  A IEG+WQRLKA+DIE  QLIF+PYGG+MSQ
Sbjct: 361 SLEPLLNRTPSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRMSQ 420

Query: 421 ISESETPFSHRAGNLYKIGYVVAWKEQSVDAKKRHLSWIRELYDYMAPFVSNSPRAAYAN 480
           ISE ETPFSHRAG LYKIGY VAWKEQS+ AKK+H+SWIR++Y YM PFVS SPR+AYAN
Sbjct: 421 ISEMETPFSHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAYAN 480

Query: 481 YRDLDIGTNNKYGKTSYKRARIWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTL 536
           YRDLDIG N KYGKTS ++A IWGLKYFGNNF RLV+VKTKVDPYDFFRHEQSIPTL
Sbjct: 481 YRDLDIGANKKYGKTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537

BLAST of Tan0020441 vs. TAIR 10
Match: AT4G20820.1 (FAD-binding Berberine family protein )

HSP 1 Score: 548.5 bits (1412), Expect = 5.9e-156
Identity = 273/540 (50.56%), Postives = 377/540 (69.81%), Query Frame = 0

Query: 1   MKYSSLIPLSLVFIVLS------PSPLWVAASADKHQNFLHCLSEHSSDSYSISKVVYTP 60
           MK+ S     L+F   S      P P  V+A+     +FL CLS   +DS  +SKV++TP
Sbjct: 1   MKFQSFFSSVLIFFTTSTLLLSIPHP--VSANRSNQSSFLQCLSLQLNDSNIVSKVIHTP 60

Query: 61  INSSYSSVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHD 120
            ++S+SSVL  SI+N RFS  D PKP++I+TP+  S +Q+A+ C++  G+ IRTRSGGHD
Sbjct: 61  NDTSFSSVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHD 120

Query: 121 FEGLSYVSHLPFIIVDLINLKSIEVDVKKSTAWVQSGATLGELYYTIAEKSRTLAFPAGV 180
           +EGLSYV+H PF+I+DL NL+SI VDV   + WVQ+GAT+GELYY I +K+RTLAFPAGV
Sbjct: 121 YEGLSYVTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGV 180

Query: 181 CPTVGVGGHFSGGGYGLMLRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGG 240
           CPTVGVGGHFSGGGYG +LRK+GLAAD+VIDA +VDA G+I +R  MGED FWAIRGGGG
Sbjct: 181 CPTVGVGGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGG 240

Query: 241 GSFGIVVAWKVKLVQVPATVTICSTSRKLEEDAIKLIHHWQYVANKLDENLFLGIILIGG 300
            SF +V++WK+ L+ VP+TVT+ + ++  E+ A+K+IH WQ+VA+K+ ++LF+ ++L   
Sbjct: 241 SSFCVVLSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVML--- 300

Query: 301 NISTQEGITNPTATFFSLFLGKVDELVAILKTNFPELGLIKEDCVETSWIESTL-IAATG 360
               Q       A+F  L+LG V  L+ ++   FPELGL ++DC E SWIES +  A  G
Sbjct: 301 ----QRYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWFAELG 360

Query: 361 VQTLESLEPLLNRTPPTNESTKIKSDYVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYG 420
            + +     +L +    + + K KSD+V+EP+P+ AI  +W+RL+  + E  QLIF P+G
Sbjct: 361 EEPIN----VLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFG 420

Query: 421 GKMSQISESETPFSHRAGNLYKIGYVVAWKEQSVDAKKRHLSWIRELYDYMAPFVSNSPR 480
           GKMS+I++ ETPF HR GN+Y+I Y+  W+    D K++++ W+  +YD M+ FV+ SPR
Sbjct: 421 GKMSEIADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPR 480

Query: 481 AAYANYRDLDIGTNNKYGKTSYKRARIWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIP 534
            AY N RDLD+G      ++ Y+  + WG+KYF NNF RLV VKT VDP DFF  EQSIP
Sbjct: 481 GAYINLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524

BLAST of Tan0020441 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 540.8 bits (1392), Expect = 1.2e-153
Identity = 272/540 (50.37%), Postives = 382/540 (70.74%), Query Frame = 0

Query: 1   MKYSSLIPLSLVFIVLSPSPLWVAASADKHQNFLHCLSEHSSDSYSISKVVYTPINSSYS 60
           M++SS +  +++   L+ SPL V+A    H++FL CLS   +D+    KV++T  +SS+ 
Sbjct: 1   MEFSSFL-FTILLFSLNISPL-VSAHGSNHEDFLKCLSYRMNDNTVEPKVIHTSKDSSFF 60

Query: 61  SVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSY 120
           S+LD SI+N RFS ++TPKP+ IITP+  S +Q  I C++ HG+ +RTRS GH +EGLSY
Sbjct: 61  SILDSSIQNPRFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSY 120

Query: 121 VSH-LPFIIVDLINLKSIEVDVKKSTAWVQSGATLGELYYTIAEKSRTLAFPAGVCPTVG 180
           +++  PF ++DL NL+SI +DV   T WVQ+GAT GELYY I + +++LAFPAG+ PTVG
Sbjct: 121 IAYNKPFAVIDLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVG 180

Query: 181 VGGHFSGGGYGLMLRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGI 240
           VGG FSGGGYG +LRKYGLAADN+IDA +VDA+G+I DR++MGED FWAIRGGGG SFG+
Sbjct: 181 VGGQFSGGGYGTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGV 240

Query: 241 VVAWKVKLVQVPATVTICSTSRKLEEDAIKLIHHWQYVANKLDENLFLGIILIGGNISTQ 300
           +++WKVKLV VP+T+T+    +  +++A+++I  WQY A+K+ ++LF+   L   N +  
Sbjct: 241 ILSWKVKLVDVPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFIRTTLERSNKNAV 300

Query: 301 EGITNPTATFFSLFLGKVDELVAILKTNFPELGLIKEDCVETSWIESTLIAATGVQTLES 360
                  A F  L++G V+ L+A+++  FPELGL KE C E SWIES L  A   +  ES
Sbjct: 301 H------ALFTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIESVLWFADFPKG-ES 360

Query: 361 LEPLLNRTPPTNESTKIKSDYVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKMSQI 420
           L  L NR   T+ S K K D+V+EPIPEAAI+ IW+RL+A +  + ++I  P+GGKMS++
Sbjct: 361 LGVLTNR-ERTSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEM 420

Query: 421 SESETPFSHRAGNLYKIGYVVAWKEQSVDAK---KRHLSWIRELYDYMAPFVSNSPRAAY 480
           +E ETPF HR GNLY+I YV  W+E+    K    ++L W+  +Y++M P+VS SPR AY
Sbjct: 421 AEYETPFPHRGGNLYEIQYVAYWREEEDKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAY 480

Query: 481 ANYRDLDIGTNNKYGKTSYKRARIWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTLS 537
            N++D+D+G      KT Y+  + WG+KYF NNF RLV VKT+VDP DFF  EQSIP ++
Sbjct: 481 VNFKDMDLGMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIPLVN 530

BLAST of Tan0020441 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 538.9 bits (1387), Expect = 4.7e-153
Identity = 272/540 (50.37%), Postives = 371/540 (68.70%), Query Frame = 0

Query: 1   MKYSSLIPLSLVFIVLSPSPLWVAASADKHQNFLHCLSEHSSDSYSISKVVYTPINSSYS 60
           MKY+ ++ L  V  +   S     +S+   + F  CL+ +S   + IS  ++   N SYS
Sbjct: 1   MKYALILVLFFVVFIWQSS-----SSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYS 60

Query: 61  SVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSY 120
           SVL  +IRNLRF+T  TPKP +II   + SH+QAAI C K H LQ++ RSGGHD++GLSY
Sbjct: 61  SVLQANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSY 120

Query: 121 VSH--LPFIIVDLINLKSIEVDVKKSTAWVQSGATLGELYYTIAEKSRTLAFPAGVCPTV 180
           V++   PF ++D+ NL+S++VDV   TAWVQ+GA LGE+YY I EKS+TLA+PAG+CPTV
Sbjct: 121 VTYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTV 180

Query: 181 GVGGHFSGGGYGLMLRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFG 240
           GVGGH SGGGYG M+RKYGL  DN IDA +VD NGKI DR+ MGEDL+WAI GGGGGS+G
Sbjct: 181 GVGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYG 240

Query: 241 IVVAWKVKLVQVPATVTICSTSRKLEEDAIKLIHHWQYVANKLDENLFLGIIL--IGGNI 300
           +V+A+K+ LV+VP  VT+   SR LE++A  +IH WQ VA KL + LF+  ++  + G +
Sbjct: 241 VVLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTV 300

Query: 301 STQEGITNPTATFFSLFLGKVDELVAILKTNFPELGLIKEDCVETSWIESTLIAATGVQT 360
           S+Q+ +     TF ++FLG    L++IL   FPELGL++ DC ETSWI+S L   T +Q 
Sbjct: 301 SSQKTV---RTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLF-WTNIQV 360

Query: 361 LESLEPLLNRTPPTNESTKIKSDYVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKM 420
             S   LL R  P N   K KSDYV+EPI    +E IW+  K  ++E+P + F PYGG+M
Sbjct: 361 GSSETLLLQRNQPVN-YLKRKSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEM 420

Query: 421 SQISESETPFSHRAGNLYKIGYVVAWKEQSVDAKKRHLSWIRELYDYMAPFVSNSPRAAY 480
            +IS + TPF +RAGNL+KI Y   W+++++    R++   R+LY +M PFVS +PR ++
Sbjct: 421 GRISSTVTPFPYRAGNLWKIQYGANWRDETL--TDRYMELTRKLYQFMTPFVSKNPRQSF 480

Query: 481 ANYRDLDIGTNNKYGK-TSYKRARIWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTL 536
            NYRD+D+G N+  GK +SY   + +G KYF  NF RLV +KT+VD  +FFR+EQSIP L
Sbjct: 481 FNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of Tan0020441 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 519.2 bits (1336), Expect = 3.9e-147
Identity = 259/541 (47.87%), Postives = 374/541 (69.13%), Query Frame = 0

Query: 6   LIPLSLVFIVLS------PSPLWVAASADKHQNFLHCLSEHSSDSYSISKVVYTPINSSY 65
           L+ L + F++L+      PS   ++A    H+NFL CLS   ++    S++++T  + SY
Sbjct: 7   LLSLFIYFLLLNLSLSHFPS---ISAQRTNHENFLKCLSHRINE--DDSRIIHTSKDPSY 66

Query: 66  SSVLDFSIRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLS 125
            S+L+ SI+N RF   +TPKP+ IITP+  + +Q+ I C++ HG+ IRTRSGGHD+EGLS
Sbjct: 67  FSILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLS 126

Query: 126 YVS-HLPFIIVDLINLKSIEVDVKKSTAWVQSGATLGELYYTIAEKSRTLAFPAGVCPTV 185
           Y++   PF+++DL NL+SI +DV   T WVQSGAT+GELYY I + S++LAFPAG+ PTV
Sbjct: 127 YMAKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTV 186

Query: 186 GVGGHFSGGGYGLMLRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFG 245
           G+GG F GGGYG ++RKYGL+ADNVIDA +VDANG   DR+ MGED FWAIRGGGG SF 
Sbjct: 187 GIGGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFS 246

Query: 246 IVVAWKVKLVQVPATVTICSTSRKLEEDAIKLIHHWQYVANKLDENLFLGIILIGGNIST 305
           +V++WK++L+ VP+ VT+    +  E++A+ +I+ WQY+A+K+  +LF+  +L       
Sbjct: 247 VVLSWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLFIRAML------- 306

Query: 306 QEGITNPTATFFSLFLGKVDELVAILKTNFPELGLIKEDCVETSWIESTLIAATGVQTLE 365
            +  T   A+F  L+LG V +L+A++K  FPELGL   +C E SWIES L    G    E
Sbjct: 307 -QKETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLWFIKG----E 366

Query: 366 SLEPLLNRTPPTNESTKIKSDYVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKMSQ 425
           S+E +L +   T+ S K K D+++EPIP+ AI+ +W+R +A +  + ++I  P+GGKMS+
Sbjct: 367 SME-ILAKRKRTSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSE 426

Query: 426 ISESETPFSHRAGNLYKIGYVVAWKEQSVDAK---KRHLSWIRELYDYMAPFVSNSPRAA 485
           I+++E PF HR GNLY+I Y+  W E+    K   +++L W+  +Y++M P+VS SPR A
Sbjct: 427 IADNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRA 486

Query: 486 YANYRDLDIGTNNKYG-KTSYKRARIWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPT 536
           Y N+RD+D+G       KT Y+ A++WG+KYF NNF+RLV VKT VDP DFF  EQSIP 
Sbjct: 487 YVNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQSIPI 529

BLAST of Tan0020441 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 517.7 bits (1332), Expect = 1.1e-146
Identity = 259/533 (48.59%), Postives = 369/533 (69.23%), Query Frame = 0

Query: 9   LSLVFIVLSPSPLWVAASADKHQNFLHCLSEHSSDSYSISKVVYTPIN--SSYSSVLDFS 68
           L L F + + +P   ++ A     F++C+  ++   + + K  + P    S +S VL+ +
Sbjct: 13  LVLYFSLYTITP--TSSLASLQDQFINCVQRNTHVYFPLEKTFFAPTKNVSMFSQVLEST 72

Query: 69  IRNLRFSTADTPKPLVIITPLYVSHIQAAIICSKNHGLQIRTRSGGHDFEGLSYVSHL-- 128
            +NLRF     PKP  I +P++ SH+QA+IICSK   + +R RSGGHD+EGLSYVS +  
Sbjct: 73  AQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGHDYEGLSYVSQIDK 132

Query: 129 PFIIVDLINLKSIEVDVKKSTAWVQSGATLGELYYTIAEKSRTLAFPAGVCPTVGVGGHF 188
           PFI++DL  ++ + ++++ ++AWVQSGAT+GELYY IAEKS+   FPAG+C ++G+GGH 
Sbjct: 133 PFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHI 192

Query: 189 SGGGYGLMLRKYGLAADNVIDATLVDANGKIHDRESMGEDLFWAIRGGGGGSFGIVVAWK 248
           +GG YG M+RKYGL ADNV+DA +VDANGK+ DR +MGED FWAIRGG GGSFGI++AWK
Sbjct: 193 TGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWK 252

Query: 249 VKLVQVPATVTICSTSRKLEED-AIKLIHHWQYVANKLDENLFLGIILIGGNISTQEGIT 308
           +KLV VP TVT+ + ++ L++D   K+I  WQ VA+KL E LF+ ++    N++   G  
Sbjct: 253 IKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLF---NVAGTGGNK 312

Query: 309 NPTATFFSLFLGKVDELVAILKTNFPELGLIKEDCVETSWIESTLIAATGVQTLESLEPL 368
             T ++ +LFLG    L+ ++K +FPELGL  +DC+E SW+ES +   +G  T      L
Sbjct: 313 TVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLES-IAYISGFPTHTPTNVL 372

Query: 369 L-NRTPPTNESTKIKSDYVKEPIPEAAIEGIWQRLKAQDIEVPQLIFVPYGGKMSQISES 428
           L  ++P    S K KSD+VK PIPE+ ++GI+++L  +DI  P +I+ PYGG M++I ES
Sbjct: 373 LQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDI--PLMIWNPYGGMMAKIPES 432

Query: 429 ETPFSHRAGNLYKIGYVVAWKEQSVDAKKRHLSWIRELYDYMAPFVSNSPRAAYANYRDL 488
           + PF HR G L+K+ YV +W + S     RH++WIR+LY YM P+VS++PR AY NYRDL
Sbjct: 433 QIPFPHRKGVLFKVQYVTSWLD-SDKRPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDL 492

Query: 489 DIGTNNKYGKTSYKRARIWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPTL 536
           D+G N K  KT  K+A++WG  YF NNFNRL+ +K KVDP +FFRHEQSIP +
Sbjct: 493 DLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPM 536