Tan0020410 (gene) Snake gourd v1

Overview
NameTan0020410
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionMalate dehydrogenase [NADP], chloroplastic
LocationLG04: 5514317 .. 5519491 (-)
RNA-Seq ExpressionTan0020410
SyntenyTan0020410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTCTTCATCGTCCCGTTCGACACGTGTCACCCGCGACGCCTTACAAAATCATTTCAAATGATCCTTTTCTCTCTTCGTCCATTTTTCTCAACCTCTATCCAAACCTCTCCCGCTCCCAAACCCCATAGCCAAAGAGCTTCACTTCTTCTTTCTACTCTCAATCCTCTCTCTACTCTGAATAAGCTCCATTCCCCCATTTCTGTCATCTCCCTTAGCTCCCATCTCTCCCTGCTGCAATGGCTGTAGCAGAGTTGTCATCTTCTTTAAAAACTGAGCTCAACTTATCATCCAAGATTGTTCTTCAGTCGAATCAGTTCTCTAATCACCGTCGATGCTCTTTTCGGCCGTTCTATAGAACCAGAAGTGCCCGAATCACTTGCTCTATTGCGCCCAAGTGAGCTTCGTTTGTGTTGAGTCTTATGTGTTGATGTAAATTTGTGCTTCTCTATGTGTTTGTGAGTAAGTTTTGAAGATTTTTCTATGTTGAATTTTTGAGAAATGACCAACGGATTGTTTGCCCTGTTCAATTTTTCAGTCAGGTTGAAGCAGCTCCGGTTGCTGCGAAAACAGAGGACCCCAAAAGTAAAAGTGATTGTTTTGGTGTTTTCTGCCTCACCTATGATTTGAAAGCTGTAAGTTCTTCAATTCTCATGGGGATATCTTTGTTTATTGAAAATGTATGATTGACTGTGTTTCAGTGGTTAAGTTTAGTTCAACTCATGAGATCAATTTTCGTCGTATGCTGCGTGTTTTTCTGTTCTTTAGGTTTTGTTTTCTTTTTAGACTATTTTCTATAACTTCTAATTACTTTATTTTTTATTTTTTTTTATTTTATGGATTGCTCGATTCTTTTGTTTGTTCATTCTGAGTAATCTAATCATCAATTGCAGACTCTGCCTCTAGCTCATTCTCATTATGAAAATACAGATGTGTAAAAATGTTTCGTGATTGTTTGAATATGAGGGGTTATTTCAATAGCAATTTGTTCTTTCTTTCACCCCATTTCTTCTTCAAAGTGTGTGTACGTACTTTATTGCAGAGCTTCAATGGTCGTAGGAATTTCATATTATTTGTCGTTTTTCTAAGATTGTCCATCATTTAGTTTATCTAACATTTTCGAACCAATACTCATTAGTTGAAATTTTAGTATTTATTGGGGTTATGTGAGTTTTCAAGGTACATAAGTTCCACTTTATAATTCTAAAAATGAGCTTCTCATAAATTTTACCCGTTCCATAACTAGATCTGGGTATGTTAATTTTAATTTCAGGAAATAGCTAATTGACTGTAGTATGGGATACAACTTGCATTTAAAAGAATTATTTGAGACAGAACCTCTAGTCTTACTGGACATTTGAATGTTCTTCTTTGTCATTCAACAAGTGATAAAAATTTGTATTTATGGTAGTATCCATATGATTTGAACACTGAAATATATTTCCTATTGACATTCTCATGTGTTGAATTCATCATATTTCATACAAATGAGATAATACGTCAACTTTGAAGAAATATATTACATTCATGTGTTGAATTCATCATATTTCTTTTGCTGCTAGTTCTGGCAGATTGGACAAACCTTTTCTTTGGGCCCTGACATATTTTATACTAACCCCAGGAAGAAGAGACAACATCATGGAAGAAAATGATTAATGTTGCAGTTTCTGGTGCTGCTGGAATGATATCCAATCACCTTCTCTTCAAGGTAAGAATTCATTTAAATCAACCTCAAAATCTCGTGATGTTTCGGCGTTTCCATGGTACTTCCCCATTCCCCTAGAGTCCTTGGCTATGTATTTTTCCTTTGTTGTACAAGCTAGGTGTTTCCCAAGCCAGTACACTAACAATTGAACCACATTTTCACTCGCACAACAAAAGAACTAAAATCTTCGCCTTTATTAATCAAGAATCTATTTGATACAAAATTGAAGGTTCAGAAACCTATTTGATACTTTTGAGTTTCAAGGATTTATTAAACACATTTTAAAGTTCAAGAACCATTAGATGCAATTTTGAAAAATCAGGGACTAAACTTGTTATTTAACTCCTTTTTGTTGATAGCTTGTTTTCTTAATAACTTTGAGTGGTAGGGAGAAATGGACTGAGTATCTTGATTCCTTTGTTGTATTTCACGTCCTAATATTTTATACTTTCTATAAAGAGGGACAATGGTGCTGTTGGTGGGATACCTTTTTTTTAGGAGAAAACTAACTTCTCTCATCTGATGGTTTGGATGAAATCTTACTTTGCTTGTTTCGCTAGCTTGCATCTGGTGAAGTTTTTGGTCCCGATCAACCAATTGCATTGAAACTATTGGGATCTGAGAGGTCATTCCAGGCACTTGAAGGCAAGTTTAGCACACTTTAAAACAATCTTCAGTATTGCTTTGAAGTTCATTTGATTGCTTGGTATTTCTGTAGCCACTCATTTTAAAATACTTAGTGCTATGAAGATAAAATAAATGAAACAGAAGTTTCTTTTGATTCTTCTATGGAGTTAGCCTGTATCAGTCTTTTTCCCTTTATGTGTTGAGAACAGTTAAAATAAGAACTTGCAAGTCTATAATGATTTAAACCACCATATTAGATTATATGGGGTTCCATCTCAAAATCAATTGACAATGAGAGGAGTAGCTCATATATTTTATGTATTGTGTGGTTTCTCTCCACTTTTCCAATGTGGGATCCTCAACACACCAAACTTTGAGATTGAACTGCAATCTTCCAGCTGCATTGACATCTCCACATGTGTAGGTGTTGCTATGGAACTGGAGGACTCCCTTTTCCCTTTATTGAGGGAGGTGGTAATAAGCATTGATCCTTATGAAGTATTCCAAGATGCAGAATGGGCTCTTTTAATAGGAGCAAAGCCTCGAGGGCCAGGAATGGAACGTGCTGGTTTATTAGATATAAACGGGCAGATATTTGCTGAACAAGTACGTTGATTGATTATTTTTTGTGATTCATTTTTTCTGCCCATCCGATAAATATCGGACTTTCTTACATTTATCTTTTGATTATCATTTAACAATGAACATAGGGCAAAGCTCTAAATGCTGTTGCATCCCCAAATGTCAAAGTCATAGTTGTGGGCAATCCCTGCAATACCAAGTGAGTTCGTGTTTTATTCAGTATGAGTCTCTTGAAGTGAGTGCAAGATATAACCTCTCTTTTCCTTATCATTCCATGTAGTGCATTAATTTGTATGAAAAATGCTCCAAAAATTCCTGCAAAGAATTTCCACGCTTTAACTCGATTAGATGAGAATCGAGCAAAATGTCAGGTACTATACCCCTTTCCTCACATGTAATATGTTTTAGAATAGATAAATCTTTCTTACTTTCTAAATATGACTTGTTCAGCTGGCTCTGAAAGCGGGCGTCTTCTATGATAAAGTGTCGAATATGACCATTTGGGGCAACCACTCTACAACTCAGGTTATCAAATAGTTTATGCTCTAGAAGCTAGCTGGCTTTTTGAAATTCTCCCAATTGTATGCTTATTTCATCATCTTTCACTCCAGGTTCCGGACTTCTTGAACGCAAGAATTAACGGGTTACCGGTTAAAGAGGTTATCAAGGACCACAGGTGGTTGGAAGAGGAATTCACTGAAAAAGTACAGAAGGTAATCTTATTAGCAAAGGATTAACAACACATACAGTGTTCACTGCTAAATGAATCAAATAGTATCTTGATCGACATGTTTACTCACGGGCCGTTGTCTCGATTTTCAGAGGGGTGGCGTGCTGATTGAGAAATGGGGAAGATCTTCAGCTGCTTCAACTGCTGTGTCAATTGTTGATGCCATAAGATCTTTAATTACTCCAACTCCTGAAGGTGATTGGTTTTCATCTGGGGTAAGCTTAAATGTCATGTAGCATCCCTTGATACTTGTGTATTATTACATTGTTTTATAAACTGATAGGCCTCTAAATAGTTGAAAATGGGGGGTAGCTAGCCGAGTAATTCTAATCGAGTGTACCGTGAGGGGGGGCACGAACAAAAATTTTGGATCTAGCTAGAGTGTTGAATTGTCTCTTAACCTAAACGTTCCCAGACTAAGAACCTCAAACTCATAAATACCGATCGATTTATAGGTATATACAACTGGAAATCCTTATGGCATAGCAGAGGATATTGTTTTCAGCATGCCGTGTAGATCAAAGGTAACTTTGTATGCCCTTTTATTCTTTCATTTGATCAATTAAGTTTCAGTTTCTAACCAAAAAAAACTTTCATTGCCTACTTTCATAACATAGTGTCTGTAATCAGTAATATGCTGCTCTCACAGGGAGATGGTGACTATGAACTTGTGAAAGATGTGATATTTGATGACTATCTCCGCAAGCGAATTGCCAAGGTCTGATATGTTTTTTTGTCTAATTCAGTAAAAGTTTGTTTGGAATAAAGCACACTGTTCAACATGGAACAAGCATCAAATTTTTCAAAGTTCTATACATATGAAACAAAATATTTCCCCTTTCTCCTTTTCCTCTTAAACAAAAATACATGCCAATGTCACGTCATTCTTCGACTTCTAATTAGATGAAGGGTGTATTTGAAATGACTTTTCAAGAGTTTAAAACATTTAAGAACTTTTTTTTAACACTTGAAGAGTCATTTCAAACGGACTCGAATTTACACAACACAATGGAAAAAAACTTTTATTCTGAAGTATTGCTACCGGATTTCGGCTATCGATGAACTGCCTAATTCATTTTATTTTGCACTGTTGTTTTACAGACTGAAGCTGAGTTGCTAGCTGAGAAGAGATGTGTGGCTCATCTCACAGGAGAGGTCAGATTTAATCTTTTTCTCTTTTTTTTGTGGGTAAATTTTCATCTTTCTGTTTAATCCTGCTTAGTGAGCATTAATCATGGATTATTTTATCCTCCTGGTATCAGGGTATTGCTGTATGTGATTTACCAGAAGACACAATGCTTCCTGGAGAAATGTAAGGCTCATAATCACATGGCTCTAAGCCCCAAAATTTTATGTTTGAACTGCTTTATGCTACTTTCATATTTGAATTAAACCACATAGTATATGTGAATATTGCTTGTAAGTTGTCAGCAATGATAGTAAAATTAATAATAGTATATTGTTTTGTCTGAATTTTCCTGATATGAATCGGCAATAAAATATTGGTCAAACGTCTAAGTTTGTTGATTCCTA

mRNA sequence

GTTCTTCATCGTCCCGTTCGACACGTGTCACCCGCGACGCCTTACAAAATCATTTCAAATGATCCTTTTCTCTCTTCGTCCATTTTTCTCAACCTCTATCCAAACCTCTCCCGCTCCCAAACCCCATAGCCAAAGAGCTTCACTTCTTCTTTCTACTCTCAATCCTCTCTCTACTCTGAATAAGCTCCATTCCCCCATTTCTGTCATCTCCCTTAGCTCCCATCTCTCCCTGCTGCAATGGCTGTAGCAGAGTTGTCATCTTCTTTAAAAACTGAGCTCAACTTATCATCCAAGATTGTTCTTCAGTCGAATCAGTTCTCTAATCACCGTCGATGCTCTTTTCGGCCGTTCTATAGAACCAGAAGTGCCCGAATCACTTGCTCTATTGCGCCCAATCAGGTTGAAGCAGCTCCGGTTGCTGCGAAAACAGAGGACCCCAAAAGTAAAAGTGATTGTTTTGGTGTTTTCTGCCTCACCTATGATTTGAAAGCTGAAGAAGAGACAACATCATGGAAGAAAATGATTAATGTTGCAGTTTCTGGTGCTGCTGGAATGATATCCAATCACCTTCTCTTCAAGCTTGCATCTGGTGAAGTTTTTGGTCCCGATCAACCAATTGCATTGAAACTATTGGGATCTGAGAGGTCATTCCAGGCACTTGAAGGTGTTGCTATGGAACTGGAGGACTCCCTTTTCCCTTTATTGAGGGAGGTGGTAATAAGCATTGATCCTTATGAAGTATTCCAAGATGCAGAATGGGCTCTTTTAATAGGAGCAAAGCCTCGAGGGCCAGGAATGGAACGTGCTGGTTTATTAGATATAAACGGGCAGATATTTGCTGAACAAGGCAAAGCTCTAAATGCTGTTGCATCCCCAAATGTCAAAGTCATAGTTGTGGGCAATCCCTGCAATACCAATGCATTAATTTGTATGAAAAATGCTCCAAAAATTCCTGCAAAGAATTTCCACGCTTTAACTCGATTAGATGAGAATCGAGCAAAATGTCAGCTGGCTCTGAAAGCGGGCGTCTTCTATGATAAAGTGTCGAATATGACCATTTGGGGCAACCACTCTACAACTCAGGTTCCGGACTTCTTGAACGCAAGAATTAACGGGTTACCGGTTAAAGAGGTTATCAAGGACCACAGGTGGTTGGAAGAGGAATTCACTGAAAAAGTACAGAAGAGGGGTGGCGTGCTGATTGAGAAATGGGGAAGATCTTCAGCTGCTTCAACTGCTGTGTCAATTGTTGATGCCATAAGATCTTTAATTACTCCAACTCCTGAAGGTGATTGGTTTTCATCTGGGGTATATACAACTGGAAATCCTTATGGCATAGCAGAGGATATTGTTTTCAGCATGCCGTGTAGATCAAAGGGAGATGGTGACTATGAACTTGTGAAAGATGTGATATTTGATGACTATCTCCGCAAGCGAATTGCCAAGACTGAAGCTGAGTTGCTAGCTGAGAAGAGATGTGTGGCTCATCTCACAGGAGAGGGTATTGCTGTATGTGATTTACCAGAAGACACAATGCTTCCTGGAGAAATGTAAGGCTCATAATCACATGGCTCTAAGCCCCAAAATTTTATGTTTGAACTGCTTTATGCTACTTTCATATTTGAATTAAACCACATAGTATATGTGAATATTGCTTGTAAGTTGTCAGCAATGATAGTAAAATTAATAATAGTATATTGTTTTGTCTGAATTTTCCTGATATGAATCGGCAATAAAATATTGGTCAAACGTCTAAGTTTGTTGATTCCTA

Coding sequence (CDS)

ATGGCTGTAGCAGAGTTGTCATCTTCTTTAAAAACTGAGCTCAACTTATCATCCAAGATTGTTCTTCAGTCGAATCAGTTCTCTAATCACCGTCGATGCTCTTTTCGGCCGTTCTATAGAACCAGAAGTGCCCGAATCACTTGCTCTATTGCGCCCAATCAGGTTGAAGCAGCTCCGGTTGCTGCGAAAACAGAGGACCCCAAAAGTAAAAGTGATTGTTTTGGTGTTTTCTGCCTCACCTATGATTTGAAAGCTGAAGAAGAGACAACATCATGGAAGAAAATGATTAATGTTGCAGTTTCTGGTGCTGCTGGAATGATATCCAATCACCTTCTCTTCAAGCTTGCATCTGGTGAAGTTTTTGGTCCCGATCAACCAATTGCATTGAAACTATTGGGATCTGAGAGGTCATTCCAGGCACTTGAAGGTGTTGCTATGGAACTGGAGGACTCCCTTTTCCCTTTATTGAGGGAGGTGGTAATAAGCATTGATCCTTATGAAGTATTCCAAGATGCAGAATGGGCTCTTTTAATAGGAGCAAAGCCTCGAGGGCCAGGAATGGAACGTGCTGGTTTATTAGATATAAACGGGCAGATATTTGCTGAACAAGGCAAAGCTCTAAATGCTGTTGCATCCCCAAATGTCAAAGTCATAGTTGTGGGCAATCCCTGCAATACCAATGCATTAATTTGTATGAAAAATGCTCCAAAAATTCCTGCAAAGAATTTCCACGCTTTAACTCGATTAGATGAGAATCGAGCAAAATGTCAGCTGGCTCTGAAAGCGGGCGTCTTCTATGATAAAGTGTCGAATATGACCATTTGGGGCAACCACTCTACAACTCAGGTTCCGGACTTCTTGAACGCAAGAATTAACGGGTTACCGGTTAAAGAGGTTATCAAGGACCACAGGTGGTTGGAAGAGGAATTCACTGAAAAAGTACAGAAGAGGGGTGGCGTGCTGATTGAGAAATGGGGAAGATCTTCAGCTGCTTCAACTGCTGTGTCAATTGTTGATGCCATAAGATCTTTAATTACTCCAACTCCTGAAGGTGATTGGTTTTCATCTGGGGTATATACAACTGGAAATCCTTATGGCATAGCAGAGGATATTGTTTTCAGCATGCCGTGTAGATCAAAGGGAGATGGTGACTATGAACTTGTGAAAGATGTGATATTTGATGACTATCTCCGCAAGCGAATTGCCAAGACTGAAGCTGAGTTGCTAGCTGAGAAGAGATGTGTGGCTCATCTCACAGGAGAGGGTATTGCTGTATGTGATTTACCAGAAGACACAATGCTTCCTGGAGAAATGTAA

Protein sequence

MAVAELSSSLKTELNLSSKIVLQSNQFSNHRRCSFRPFYRTRSARITCSIAPNQVEAAPVAAKTEDPKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKNAPKIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEVIKDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSSGVYTTGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLTGEGIAVCDLPEDTMLPGEM
Homology
BLAST of Tan0020410 vs. ExPASy Swiss-Prot
Match: O48902 (Malate dehydrogenase [NADP], chloroplastic OS=Medicago sativa OX=3879 GN=MDH1 PE=2 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 1.1e-218
Identity = 376/439 (85.65%), Postives = 407/439 (92.71%), Query Frame = 0

Query: 1   MAVAELSSSL-KTELNLSSKIVLQSNQFSNHRRCSFRPFYRTRSARITCSIAPNQVEAAP 60
           MA+ +L+++  KT+L+ SS++   S     H  C+  P +RT+ ARI+CS+APNQV+A  
Sbjct: 1   MALTQLNNTCSKTQLHSSSQLSFLSRTLPRHHHCTLAPLHRTQHARISCSVAPNQVQAP- 60

Query: 61  VAAKTEDPKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFKLASGE 120
            A +T+DPKSK DC+GVFCLTYDLKAEEET SWKK+I +AVSGAAGMISNHLLFKLASGE
Sbjct: 61  -AVQTQDPKSKPDCYGVFCLTYDLKAEEETKSWKKLITIAVSGAAGMISNHLLFKLASGE 120

Query: 121 VFGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIG 180
           VFGP+QPIALKLLGSERS QALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIG
Sbjct: 121 VFGPNQPIALKLLGSERSLQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIG 180

Query: 181 AKPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKNAPKIP 240
           AKPRGPGMERA LLDINGQIFAEQGKALNAVAS NVKVIVVGNPCNTNALIC+KNAP IP
Sbjct: 181 AKPRGPGMERAALLDINGQIFAEQGKALNAVASRNVKVIVVGNPCNTNALICLKNAPNIP 240

Query: 241 AKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEV 300
           AKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARI+GLPVKEV
Sbjct: 241 AKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARIDGLPVKEV 300

Query: 301 IKDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSSGVY 360
           IKDH+WLEEEFTEKVQKRGG LI+KWGRSSAAST+VSIVDAIRSLI PTPEGDWFS+GVY
Sbjct: 301 IKDHKWLEEEFTEKVQKRGGALIQKWGRSSAASTSVSIVDAIRSLIIPTPEGDWFSTGVY 360

Query: 361 TTGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLT 420
           TTGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLR+++AKTEAELLAEK+CVAHLT
Sbjct: 361 TTGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRQKLAKTEAELLAEKKCVAHLT 420

Query: 421 GEGIAVCDLPEDTMLPGEM 439
           GEGIAVCDLP DTMLPGEM
Sbjct: 421 GEGIAVCDLPGDTMLPGEM 437

BLAST of Tan0020410 vs. ExPASy Swiss-Prot
Match: P21528 (Malate dehydrogenase [NADP], chloroplastic OS=Pisum sativum OX=3888 PE=1 SV=2)

HSP 1 Score: 749.2 bits (1933), Expect = 2.6e-215
Identity = 374/443 (84.42%), Postives = 406/443 (91.65%), Query Frame = 0

Query: 1   MAVAELSSSL-KTELNLSSKIVL----QSNQFSNHRRCSFRPFYRTRSARITCSIAPNQV 60
           MA+ +L+S+  K +L+ SS++      ++     H   +F P +RT+ ARI+CS+APNQV
Sbjct: 1   MALTQLNSTCSKPQLHSSSQLSFLSRTRTRTLPRHYHSTFAPLHRTQHARISCSVAPNQV 60

Query: 61  EAAPVAAKTEDPKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFKL 120
           +    AA+T+DPK K DC+GVFCLTYDLKAEEET SWKK+IN+AVSGAAGMISNHLLFKL
Sbjct: 61  QVP--AAQTQDPKGKPDCYGVFCLTYDLKAEEETKSWKKLINIAVSGAAGMISNHLLFKL 120

Query: 121 ASGEVFGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWA 180
           ASGEVFGPDQPIALKLLGSERS QALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWA
Sbjct: 121 ASGEVFGPDQPIALKLLGSERSIQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWA 180

Query: 181 LLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKNA 240
           LLIGAKPRGPG+ERA LLDINGQIFAEQGKALNAVAS N KVIVVGNPCNTNALIC+KNA
Sbjct: 181 LLIGAKPRGPGVERAALLDINGQIFAEQGKALNAVASRNAKVIVVGNPCNTNALICLKNA 240

Query: 241 PKIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLP 300
           P IPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARI+GLP
Sbjct: 241 PNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARIDGLP 300

Query: 301 VKEVIKDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFS 360
           VKEVIKD++WLEEEFTEKVQKRGGVLI+KWGRSSAAST+VSIVDAIRSLITPTPEGDWFS
Sbjct: 301 VKEVIKDNKWLEEEFTEKVQKRGGVLIQKWGRSSAASTSVSIVDAIRSLITPTPEGDWFS 360

Query: 361 SGVYTTGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCV 420
           SGVYT GNPYGIAEDIVFSMPCRSKGDGDYELV DVIFDDYLR+++AKTEAELLAEK+CV
Sbjct: 361 SGVYTNGNPYGIAEDIVFSMPCRSKGDGDYELVNDVIFDDYLRQKLAKTEAELLAEKKCV 420

Query: 421 AHLTGEGIAVCDLPEDTMLPGEM 439
           AHLTGEGIAVCDLP DTMLPGEM
Sbjct: 421 AHLTGEGIAVCDLPGDTMLPGEM 441

BLAST of Tan0020410 vs. ExPASy Swiss-Prot
Match: Q05145 (Malate dehydrogenase [NADP], chloroplastic OS=Mesembryanthemum crystallinum OX=3544 GN=MDH1 PE=2 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 7.2e-205
Identity = 359/442 (81.22%), Postives = 388/442 (87.78%), Query Frame = 0

Query: 1   MAVAELSSSLKTELNLSSKIVLQ-SNQFSNHRRCSFRPFYRTRSAR--ITCSIAPNQVEA 60
           MAVAELS S KT+L    ++    S + S+HR+ S R   R  S R  I CS+APNQV+ 
Sbjct: 1   MAVAELSPSYKTQLKTCQQLSSSLSTRLSDHRKFSLRLLPRPVSVRGGIRCSVAPNQVQ- 60

Query: 61  APVAAKTEDPKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFKLAS 120
           APVA   E    K +C+G+FCLTYDLKAEEET +WKKMI +AVSGAAGMISNHLLFKLAS
Sbjct: 61  APVAVPAEGQTGKPECYGIFCLTYDLKAEEETKTWKKMITIAVSGAAGMISNHLLFKLAS 120

Query: 121 GEVFGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALL 180
           GEVFGPDQPIALKLLGSERSF ALEGVAMELEDSL+PLLR V I IDPY++FQDAEWALL
Sbjct: 121 GEVFGPDQPIALKLLGSERSFNALEGVAMELEDSLYPLLRAVSIGIDPYDIFQDAEWALL 180

Query: 181 IGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKNAPK 240
           IGAKPRGPGMERA LLDINGQIFAEQGKALNAVAS NVKVIVVGNPCNTNALIC+KNAP 
Sbjct: 181 IGAKPRGPGMERADLLDINGQIFAEQGKALNAVASRNVKVIVVGNPCNTNALICLKNAPN 240

Query: 241 IPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVK 300
           IPAKNFH LTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNA+I+GLPVK
Sbjct: 241 IPAKNFHGLTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNAKIDGLPVK 300

Query: 301 EVIKDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSSG 360
            VIKDH+WLEEEFT  +QKRGG LI+KWGRSSAASTAVSI DAI+SL+TPTPEGDWFSS 
Sbjct: 301 TVIKDHKWLEEEFTVMIQKRGGALIQKWGRSSAASTAVSIADAIKSLVTPTPEGDWFSSA 360

Query: 361 VYTTGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAH 420
           VYT GNPYGIAED+VFSMPCRSKGDGDYELVKDV+FDDYLR+RI K+E ELLAEKRC AH
Sbjct: 361 VYTNGNPYGIAEDLVFSMPCRSKGDGDYELVKDVVFDDYLRQRIKKSEEELLAEKRCTAH 420

Query: 421 LTGEGIAVCDLPE-DTMLPGEM 439
           LTGEG+AVCDLP  DTMLPGEM
Sbjct: 421 LTGEGVAVCDLPAGDTMLPGEM 441

BLAST of Tan0020410 vs. ExPASy Swiss-Prot
Match: Q8H1E2 (Malate dehydrogenase [NADP], chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g58330 PE=2 SV=1)

HSP 1 Score: 695.3 bits (1793), Expect = 4.5e-199
Identity = 358/445 (80.45%), Postives = 387/445 (86.97%), Query Frame = 0

Query: 1   MAVAELSSSLKTE--LNLSSKIVLQSN-QFSNHRRCSFRP--FYRTRSARITCSIAPNQV 60
           MA+AELS+   T   LN SS++ L S    SNH R    P     T +++I+CS++ N  
Sbjct: 1   MAMAELSTPKTTSPFLNSSSRLRLSSKLHLSNHFRHLLLPPLHTTTPNSKISCSVSQN-- 60

Query: 61  EAAPVAAKTED-PKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFK 120
             APVA +     K+K +C+GVFCLTYDLKAEEET SWKK+IN+AVSGAAGMISNHLLFK
Sbjct: 61  SQAPVAVQENGLVKTKKECYGVFCLTYDLKAEEETRSWKKLINIAVSGAAGMISNHLLFK 120

Query: 121 LASGEVFGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEW 180
           LASGEVFGPDQPIALKLLGSERS QALEGVAMELEDSLFPLLREV I  DP EVFQD EW
Sbjct: 121 LASGEVFGPDQPIALKLLGSERSIQALEGVAMELEDSLFPLLREVDIGTDPNEVFQDVEW 180

Query: 181 ALLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKN 240
           A+LIGAKPRGPGMERA LLDINGQIFAEQGKALN  ASPNVKV+VVGNPCNTNALIC+KN
Sbjct: 181 AILIGAKPRGPGMERADLLDINGQIFAEQGKALNKAASPNVKVLVVGNPCNTNALICLKN 240

Query: 241 APKIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGL 300
           AP IPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGL
Sbjct: 241 APNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGL 300

Query: 301 PVKEVIKDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWF 360
           PVKEVI DH+WLEE FTE VQKRGG+LI+KWGRSSAASTAVSIVDAI+SL+TPTPEGDWF
Sbjct: 301 PVKEVITDHKWLEEGFTESVQKRGGLLIQKWGRSSAASTAVSIVDAIKSLVTPTPEGDWF 360

Query: 361 SSGVYTTGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRC 420
           S+GVYT GNPYGI E +VFSMPCRSKGDGDYELVKDV  DDYLR+RIAK+EAELLAEKRC
Sbjct: 361 STGVYTDGNPYGIEEGLVFSMPCRSKGDGDYELVKDVEIDDYLRQRIAKSEAELLAEKRC 420

Query: 421 VAHLTGEGIAVCDL-PEDTMLPGEM 439
           VAHLTGEGIA CDL P DTMLPGE+
Sbjct: 421 VAHLTGEGIAYCDLGPVDTMLPGEV 443

BLAST of Tan0020410 vs. ExPASy Swiss-Prot
Match: P46489 (Malate dehydrogenase [NADP], chloroplastic OS=Flaveria bidentis OX=4224 PE=1 SV=1)

HSP 1 Score: 691.8 bits (1784), Expect = 5.0e-198
Identity = 348/442 (78.73%), Postives = 380/442 (85.97%), Query Frame = 0

Query: 4   AELSSSLKTELNLSSKIVLQSNQFSNHRRCSFRPFYR-------TRSARITCSIAPNQVE 63
           +E+ SS  +  + SS      N  S+ R   + P+ R       +  A I CS+  +   
Sbjct: 17  SEIKSSSSSSSSKSSLSAYVINVSSSPRLSFYNPYPRRLHHQRLSSPASIRCSVTSSDQI 76

Query: 64  AAPVAAKTEDPKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFKLA 123
            AP+ A     K K +CFGVFCLTYDLKAEEET SWKK+INVAVSGAAGMISNHLLFKLA
Sbjct: 77  QAPLPA-----KQKPECFGVFCLTYDLKAEEETKSWKKIINVAVSGAAGMISNHLLFKLA 136

Query: 124 SGEVFGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWAL 183
           SGEVFGPDQPI+LKLLGSERSF ALEGVAMELEDSL+PLLR+V I IDPYE+FQDAEWAL
Sbjct: 137 SGEVFGPDQPISLKLLGSERSFAALEGVAMELEDSLYPLLRQVSIGIDPYEIFQDAEWAL 196

Query: 184 LIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKNAP 243
           LIGAKPRGPGMERA LLDINGQIFAEQGKALNAVASPNVKV+VVGNPCNTNALIC+KNAP
Sbjct: 197 LIGAKPRGPGMERADLLDINGQIFAEQGKALNAVASPNVKVMVVGNPCNTNALICLKNAP 256

Query: 244 KIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPV 303
            IP KNFHALTRLDENRAKCQLALKAGVFYDKVSN+TIWGNHSTTQVPDFLNA+I+G+PV
Sbjct: 257 NIPPKNFHALTRLDENRAKCQLALKAGVFYDKVSNVTIWGNHSTTQVPDFLNAKIHGIPV 316

Query: 304 KEVIKDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSS 363
            EVI+D +WLE+EFT  VQ RGGVLI+KWGRSSAASTAVSIVDAIRSL+TPTPEGDWFS+
Sbjct: 317 TEVIRDRKWLEDEFTNMVQTRGGVLIKKWGRSSAASTAVSIVDAIRSLVTPTPEGDWFST 376

Query: 364 GVYTTGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVA 423
           GVYT GNPYGIAEDIVFSMPCRSKGDGDYE VKDVIFDDYL K+I K+E ELLAEK+CVA
Sbjct: 377 GVYTNGNPYGIAEDIVFSMPCRSKGDGDYEFVKDVIFDDYLSKKIKKSEDELLAEKKCVA 436

Query: 424 HLTGEGIAVCDLPEDTMLPGEM 439
           HLTGEGIAVCDLPEDTMLPGEM
Sbjct: 437 HLTGEGIAVCDLPEDTMLPGEM 453

BLAST of Tan0020410 vs. NCBI nr
Match: XP_038891999.1 (malate dehydrogenase [NADP], chloroplastic [Benincasa hispida])

HSP 1 Score: 855.1 bits (2208), Expect = 2.6e-244
Identity = 426/438 (97.26%), Postives = 432/438 (98.63%), Query Frame = 0

Query: 1   MAVAELSSSLKTELNLSSKIVLQSNQFSNHRRCSFRPFYRTRSARITCSIAPNQVEAAPV 60
           MAVAELSSSLKTELN SSK V QSN+FSNHRR SFRPFYR+RS R+TCSIAPNQVEAAPV
Sbjct: 1   MAVAELSSSLKTELNFSSKTVFQSNRFSNHRRSSFRPFYRSRSPRVTCSIAPNQVEAAPV 60

Query: 61  AAKTEDPKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFKLASGEV 120
           AAKTEDPKSKS+CFGVFCLTYDLKAEEETTSWKK+INVAVSGAAGMISNHLLFKLASGEV
Sbjct: 61  AAKTEDPKSKSECFGVFCLTYDLKAEEETTSWKKLINVAVSGAAGMISNHLLFKLASGEV 120

Query: 121 FGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIGA 180
           FGPDQPIALKLLGSERS QALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIGA
Sbjct: 121 FGPDQPIALKLLGSERSLQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIGA 180

Query: 181 KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKNAPKIPA 240
           KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALIC+KNAPKIPA
Sbjct: 181 KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICLKNAPKIPA 240

Query: 241 KNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI 300
           KNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI
Sbjct: 241 KNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI 300

Query: 301 KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSSGVYT 360
           KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSSGVYT
Sbjct: 301 KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSSGVYT 360

Query: 361 TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLTG 420
           TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLTG
Sbjct: 361 TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLTG 420

Query: 421 EGIAVCDLPEDTMLPGEM 439
           EGIAVCDLPEDTMLPGEM
Sbjct: 421 EGIAVCDLPEDTMLPGEM 438

BLAST of Tan0020410 vs. NCBI nr
Match: XP_022968193.1 (malate dehydrogenase [NADP], chloroplastic [Cucurbita maxima])

HSP 1 Score: 834.3 bits (2154), Expect = 4.7e-238
Identity = 411/438 (93.84%), Postives = 427/438 (97.49%), Query Frame = 0

Query: 1   MAVAELSSSLKTELNLSSKIVLQSNQFSNHRRCSFRPFYRTRSARITCSIAPNQVEAAPV 60
           MAVAE  SSLK++LNLSS  V Q+N+F NHRRCSFRPFYR RS+ ITCS+APNQ+EAAPV
Sbjct: 1   MAVAEFPSSLKSQLNLSSTTVFQANRFHNHRRCSFRPFYRARSSPITCSVAPNQIEAAPV 60

Query: 61  AAKTEDPKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFKLASGEV 120
           AAKT +PKSKSDC+GVFCLTYDLKAEEETTSWKKMIN+AVSGAAGMISNHLLFKLA+GEV
Sbjct: 61  AAKTGEPKSKSDCYGVFCLTYDLKAEEETTSWKKMINIAVSGAAGMISNHLLFKLAAGEV 120

Query: 121 FGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIGA 180
           FGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDA+WALLIGA
Sbjct: 121 FGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDADWALLIGA 180

Query: 181 KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKNAPKIPA 240
           KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALIC+KNAPKIPA
Sbjct: 181 KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICLKNAPKIPA 240

Query: 241 KNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI 300
           KNFHALTRLDENRAKCQLALKAGVFYD+VSNMTIWGNHSTTQVPDFLNARINGLPVKEVI
Sbjct: 241 KNFHALTRLDENRAKCQLALKAGVFYDQVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI 300

Query: 301 KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSSGVYT 360
           KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAAST+VSIVDAIRSLI PTPEGDWFSSGVY+
Sbjct: 301 KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTSVSIVDAIRSLIIPTPEGDWFSSGVYS 360

Query: 361 TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLTG 420
           TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLR RIAKTEAELLAEKRCVAHLTG
Sbjct: 361 TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRMRIAKTEAELLAEKRCVAHLTG 420

Query: 421 EGIAVCDLPEDTMLPGEM 439
           EGIAVCDLPEDTMLPGEM
Sbjct: 421 EGIAVCDLPEDTMLPGEM 438

BLAST of Tan0020410 vs. NCBI nr
Match: XP_022945464.1 (malate dehydrogenase [NADP], chloroplastic [Cucurbita moschata])

HSP 1 Score: 833.2 bits (2151), Expect = 1.1e-237
Identity = 411/438 (93.84%), Postives = 427/438 (97.49%), Query Frame = 0

Query: 1   MAVAELSSSLKTELNLSSKIVLQSNQFSNHRRCSFRPFYRTRSARITCSIAPNQVEAAPV 60
           MAVAE  SSLK++LNLSS    Q+N+F +HRRCSFRPFYR RS+ ITCS+APNQVEAAPV
Sbjct: 1   MAVAEFPSSLKSQLNLSSTTAFQANRFYDHRRCSFRPFYRARSSPITCSVAPNQVEAAPV 60

Query: 61  AAKTEDPKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFKLASGEV 120
           AAKT +PKSKSDC+GVFCLTYDLKAEEETTSWKKMIN+AVSGAAGMISNHLLFKLA+GEV
Sbjct: 61  AAKTGEPKSKSDCYGVFCLTYDLKAEEETTSWKKMINIAVSGAAGMISNHLLFKLAAGEV 120

Query: 121 FGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIGA 180
           FGPDQPIALKLLGSERS QALEGVAMELEDSLFPLLREVVISIDPYEVFQDA+WALLIGA
Sbjct: 121 FGPDQPIALKLLGSERSLQALEGVAMELEDSLFPLLREVVISIDPYEVFQDADWALLIGA 180

Query: 181 KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKNAPKIPA 240
           KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALIC+KNAPKIPA
Sbjct: 181 KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICLKNAPKIPA 240

Query: 241 KNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI 300
           KNFHALTRLDENRAKCQLALKAGVFYD+VSNMTIWGNHSTTQVPDFLNARINGLPVKEVI
Sbjct: 241 KNFHALTRLDENRAKCQLALKAGVFYDQVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI 300

Query: 301 KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSSGVYT 360
           KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAAST+VSIVDAIRSLITPTPEGDWFSSGVY+
Sbjct: 301 KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTSVSIVDAIRSLITPTPEGDWFSSGVYS 360

Query: 361 TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLTG 420
           TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLTG
Sbjct: 361 TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLTG 420

Query: 421 EGIAVCDLPEDTMLPGEM 439
           EGIAVCDLPEDTMLPGEM
Sbjct: 421 EGIAVCDLPEDTMLPGEM 438

BLAST of Tan0020410 vs. NCBI nr
Match: XP_023541500.1 (malate dehydrogenase [NADP], chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 832.8 bits (2150), Expect = 1.4e-237
Identity = 411/438 (93.84%), Postives = 426/438 (97.26%), Query Frame = 0

Query: 1   MAVAELSSSLKTELNLSSKIVLQSNQFSNHRRCSFRPFYRTRSARITCSIAPNQVEAAPV 60
           MAVAE  SSLK++LNLSS    Q+N+F NHRRCSFRPFYR RS+ ITCS+APNQVEAAPV
Sbjct: 1   MAVAEFPSSLKSQLNLSSTTAFQANRFYNHRRCSFRPFYRARSSPITCSVAPNQVEAAPV 60

Query: 61  AAKTEDPKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFKLASGEV 120
           AAKT +PKSKSDC+GVFCLTYDLK EEETTSWKKMIN+AVSGAAGMISNHLLFKLA+GEV
Sbjct: 61  AAKTGEPKSKSDCYGVFCLTYDLKDEEETTSWKKMINIAVSGAAGMISNHLLFKLAAGEV 120

Query: 121 FGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIGA 180
           FGPDQPIALKLLGSERS QALEGVAMELEDSLFPLLREVVISIDPYEVFQDA+WALLIGA
Sbjct: 121 FGPDQPIALKLLGSERSLQALEGVAMELEDSLFPLLREVVISIDPYEVFQDADWALLIGA 180

Query: 181 KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKNAPKIPA 240
           KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALIC+KNAPKIPA
Sbjct: 181 KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICLKNAPKIPA 240

Query: 241 KNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI 300
           KNFHALTRLDENRAKCQLALKAGVFYD+VSNMTIWGNHSTTQVPDFLNARINGLPVKEVI
Sbjct: 241 KNFHALTRLDENRAKCQLALKAGVFYDQVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI 300

Query: 301 KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSSGVYT 360
           KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAAST+VSIVDAIRSLITPTPEGDWFSSGVY+
Sbjct: 301 KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTSVSIVDAIRSLITPTPEGDWFSSGVYS 360

Query: 361 TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLTG 420
           TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLTG
Sbjct: 361 TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLTG 420

Query: 421 EGIAVCDLPEDTMLPGEM 439
           EGIAVCDLPEDTMLPGEM
Sbjct: 421 EGIAVCDLPEDTMLPGEM 438

BLAST of Tan0020410 vs. NCBI nr
Match: XP_022139318.1 (malate dehydrogenase [NADP], chloroplastic [Momordica charantia])

HSP 1 Score: 829.3 bits (2141), Expect = 1.5e-236
Identity = 412/438 (94.06%), Postives = 426/438 (97.26%), Query Frame = 0

Query: 1   MAVAELSSSLKTELNLSSKIVLQSNQFSNHRRCSFRPFYRTRSARITCSIAPNQVEAAPV 60
           M VAELSSSLKT+L+LSSK + QSN+F + RRCSF    RTRSAR+TCSIAPN+VEAAPV
Sbjct: 1   MGVAELSSSLKTDLSLSSKAIFQSNRFCDRRRCSFLQLSRTRSARVTCSIAPNEVEAAPV 60

Query: 61  AAKTEDPKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFKLASGEV 120
           AAKTE+PKSKS+C+GVFCLTYDLKAEEETTSWKK+INVAVSGAAGMISNHLLFKLASGEV
Sbjct: 61  AAKTEEPKSKSECYGVFCLTYDLKAEEETTSWKKLINVAVSGAAGMISNHLLFKLASGEV 120

Query: 121 FGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIGA 180
           FGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIGA
Sbjct: 121 FGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIGA 180

Query: 181 KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKNAPKIPA 240
           KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALIC+KNAPKI A
Sbjct: 181 KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICLKNAPKISA 240

Query: 241 KNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI 300
           KNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI
Sbjct: 241 KNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI 300

Query: 301 KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSSGVYT 360
           KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAAST+VSIVDAIRSLITPTPEGDWFSSGVY+
Sbjct: 301 KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTSVSIVDAIRSLITPTPEGDWFSSGVYS 360

Query: 361 TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLTG 420
            GNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRI KTEAELLAEKRCVAHLTG
Sbjct: 361 NGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIEKTEAELLAEKRCVAHLTG 420

Query: 421 EGIAVCDLPEDTMLPGEM 439
           EGIAVCDLPEDTMLPGEM
Sbjct: 421 EGIAVCDLPEDTMLPGEM 438

BLAST of Tan0020410 vs. ExPASy TrEMBL
Match: A0A6J1HXB5 (Malate dehydrogenase [NADP], chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111467500 PE=3 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 2.3e-238
Identity = 411/438 (93.84%), Postives = 427/438 (97.49%), Query Frame = 0

Query: 1   MAVAELSSSLKTELNLSSKIVLQSNQFSNHRRCSFRPFYRTRSARITCSIAPNQVEAAPV 60
           MAVAE  SSLK++LNLSS  V Q+N+F NHRRCSFRPFYR RS+ ITCS+APNQ+EAAPV
Sbjct: 1   MAVAEFPSSLKSQLNLSSTTVFQANRFHNHRRCSFRPFYRARSSPITCSVAPNQIEAAPV 60

Query: 61  AAKTEDPKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFKLASGEV 120
           AAKT +PKSKSDC+GVFCLTYDLKAEEETTSWKKMIN+AVSGAAGMISNHLLFKLA+GEV
Sbjct: 61  AAKTGEPKSKSDCYGVFCLTYDLKAEEETTSWKKMINIAVSGAAGMISNHLLFKLAAGEV 120

Query: 121 FGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIGA 180
           FGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDA+WALLIGA
Sbjct: 121 FGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDADWALLIGA 180

Query: 181 KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKNAPKIPA 240
           KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALIC+KNAPKIPA
Sbjct: 181 KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICLKNAPKIPA 240

Query: 241 KNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI 300
           KNFHALTRLDENRAKCQLALKAGVFYD+VSNMTIWGNHSTTQVPDFLNARINGLPVKEVI
Sbjct: 241 KNFHALTRLDENRAKCQLALKAGVFYDQVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI 300

Query: 301 KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSSGVYT 360
           KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAAST+VSIVDAIRSLI PTPEGDWFSSGVY+
Sbjct: 301 KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTSVSIVDAIRSLIIPTPEGDWFSSGVYS 360

Query: 361 TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLTG 420
           TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLR RIAKTEAELLAEKRCVAHLTG
Sbjct: 361 TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRMRIAKTEAELLAEKRCVAHLTG 420

Query: 421 EGIAVCDLPEDTMLPGEM 439
           EGIAVCDLPEDTMLPGEM
Sbjct: 421 EGIAVCDLPEDTMLPGEM 438

BLAST of Tan0020410 vs. ExPASy TrEMBL
Match: A0A6J1G116 (Malate dehydrogenase [NADP], chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111449691 PE=3 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 5.1e-238
Identity = 411/438 (93.84%), Postives = 427/438 (97.49%), Query Frame = 0

Query: 1   MAVAELSSSLKTELNLSSKIVLQSNQFSNHRRCSFRPFYRTRSARITCSIAPNQVEAAPV 60
           MAVAE  SSLK++LNLSS    Q+N+F +HRRCSFRPFYR RS+ ITCS+APNQVEAAPV
Sbjct: 1   MAVAEFPSSLKSQLNLSSTTAFQANRFYDHRRCSFRPFYRARSSPITCSVAPNQVEAAPV 60

Query: 61  AAKTEDPKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFKLASGEV 120
           AAKT +PKSKSDC+GVFCLTYDLKAEEETTSWKKMIN+AVSGAAGMISNHLLFKLA+GEV
Sbjct: 61  AAKTGEPKSKSDCYGVFCLTYDLKAEEETTSWKKMINIAVSGAAGMISNHLLFKLAAGEV 120

Query: 121 FGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIGA 180
           FGPDQPIALKLLGSERS QALEGVAMELEDSLFPLLREVVISIDPYEVFQDA+WALLIGA
Sbjct: 121 FGPDQPIALKLLGSERSLQALEGVAMELEDSLFPLLREVVISIDPYEVFQDADWALLIGA 180

Query: 181 KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKNAPKIPA 240
           KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALIC+KNAPKIPA
Sbjct: 181 KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICLKNAPKIPA 240

Query: 241 KNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI 300
           KNFHALTRLDENRAKCQLALKAGVFYD+VSNMTIWGNHSTTQVPDFLNARINGLPVKEVI
Sbjct: 241 KNFHALTRLDENRAKCQLALKAGVFYDQVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI 300

Query: 301 KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSSGVYT 360
           KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAAST+VSIVDAIRSLITPTPEGDWFSSGVY+
Sbjct: 301 KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTSVSIVDAIRSLITPTPEGDWFSSGVYS 360

Query: 361 TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLTG 420
           TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLTG
Sbjct: 361 TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLTG 420

Query: 421 EGIAVCDLPEDTMLPGEM 439
           EGIAVCDLPEDTMLPGEM
Sbjct: 421 EGIAVCDLPEDTMLPGEM 438

BLAST of Tan0020410 vs. ExPASy TrEMBL
Match: A0A6J1CFD8 (Malate dehydrogenase [NADP], chloroplastic OS=Momordica charantia OX=3673 GN=LOC111010262 PE=3 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 7.4e-237
Identity = 412/438 (94.06%), Postives = 426/438 (97.26%), Query Frame = 0

Query: 1   MAVAELSSSLKTELNLSSKIVLQSNQFSNHRRCSFRPFYRTRSARITCSIAPNQVEAAPV 60
           M VAELSSSLKT+L+LSSK + QSN+F + RRCSF    RTRSAR+TCSIAPN+VEAAPV
Sbjct: 1   MGVAELSSSLKTDLSLSSKAIFQSNRFCDRRRCSFLQLSRTRSARVTCSIAPNEVEAAPV 60

Query: 61  AAKTEDPKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFKLASGEV 120
           AAKTE+PKSKS+C+GVFCLTYDLKAEEETTSWKK+INVAVSGAAGMISNHLLFKLASGEV
Sbjct: 61  AAKTEEPKSKSECYGVFCLTYDLKAEEETTSWKKLINVAVSGAAGMISNHLLFKLASGEV 120

Query: 121 FGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIGA 180
           FGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIGA
Sbjct: 121 FGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIGA 180

Query: 181 KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKNAPKIPA 240
           KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALIC+KNAPKI A
Sbjct: 181 KPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICLKNAPKISA 240

Query: 241 KNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI 300
           KNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI
Sbjct: 241 KNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEVI 300

Query: 301 KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSSGVYT 360
           KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAAST+VSIVDAIRSLITPTPEGDWFSSGVY+
Sbjct: 301 KDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTSVSIVDAIRSLITPTPEGDWFSSGVYS 360

Query: 361 TGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLTG 420
            GNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRI KTEAELLAEKRCVAHLTG
Sbjct: 361 NGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIEKTEAELLAEKRCVAHLTG 420

Query: 421 EGIAVCDLPEDTMLPGEM 439
           EGIAVCDLPEDTMLPGEM
Sbjct: 421 EGIAVCDLPEDTMLPGEM 438

BLAST of Tan0020410 vs. ExPASy TrEMBL
Match: A0A0A0KTH8 (Malate dehydrogenase [NADP], chloroplastic OS=Cucumis sativus OX=3659 GN=Csa_5G622460 PE=3 SV=1)

HSP 1 Score: 822.0 bits (2122), Expect = 1.2e-234
Identity = 409/439 (93.17%), Postives = 427/439 (97.27%), Query Frame = 0

Query: 1   MAVAELSSSLKTELNLSSKIVLQSNQ-FSNHRRCSFRPFYRTRSARITCSIAPNQVEAAP 60
           MAVAELSSSLKTELN SS  +  SN+ F++HRRCSFRPF+R+R++R+TCSI  NQVEAAP
Sbjct: 1   MAVAELSSSLKTELNFSSNSLFHSNRFFADHRRCSFRPFHRSRTSRVTCSI--NQVEAAP 60

Query: 61  VAAKTEDPKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFKLASGE 120
           VAAKTEDPKSKS+C+GVFCLTYDLKAEEETTSWKK+IN++VSGAAGMISNHLLFKLASGE
Sbjct: 61  VAAKTEDPKSKSECYGVFCLTYDLKAEEETTSWKKLINISVSGAAGMISNHLLFKLASGE 120

Query: 121 VFGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIG 180
           VFGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIG
Sbjct: 121 VFGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIG 180

Query: 181 AKPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKNAPKIP 240
           AKPRGPGMERA LLDINGQIFAEQGKALNAVASPNVKVI+VGNPCNTNALIC+KNAPKIP
Sbjct: 181 AKPRGPGMERAALLDINGQIFAEQGKALNAVASPNVKVIIVGNPCNTNALICLKNAPKIP 240

Query: 241 AKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEV 300
           AKNFHALTRLDENRAKCQLALKAGVFYD+VSNMTIWGNHSTTQVPDFLNA+INGLPVKEV
Sbjct: 241 AKNFHALTRLDENRAKCQLALKAGVFYDQVSNMTIWGNHSTTQVPDFLNAKINGLPVKEV 300

Query: 301 IKDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSSGVY 360
           IKDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSSGVY
Sbjct: 301 IKDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSSGVY 360

Query: 361 TTGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLT 420
           TTGNPYGIA DIVFSMPCRSKGDGDYELV DVIFDDYLRKRIAKTEAELLAEKRCVAHLT
Sbjct: 361 TTGNPYGIANDIVFSMPCRSKGDGDYELVNDVIFDDYLRKRIAKTEAELLAEKRCVAHLT 420

Query: 421 GEGIAVCDLPEDTMLPGEM 439
           GEGIAVCDLP DTMLPGEM
Sbjct: 421 GEGIAVCDLPGDTMLPGEM 437

BLAST of Tan0020410 vs. ExPASy TrEMBL
Match: A0A5D3CB64 (Malate dehydrogenase [NADP], chloroplastic OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G002310 PE=3 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 3.4e-234
Identity = 409/439 (93.17%), Postives = 426/439 (97.04%), Query Frame = 0

Query: 1   MAVAELSSSLKTELNLSSKIVLQSNQFS-NHRRCSFRPFYRTRSARITCSIAPNQVEAAP 60
           MAVAELSSSLKTELN SS  +  SN+FS +HRRCSFRPF+R+++ R+TCSI  NQVEAAP
Sbjct: 1   MAVAELSSSLKTELNFSSNTLFHSNRFSADHRRCSFRPFHRSQTPRVTCSI--NQVEAAP 60

Query: 61  VAAKTEDPKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFKLASGE 120
           V AKTEDPKSKSDC+GVFCLTYDLKAEEETTSWKK+INV+VSGAAGMISNHLLFKLASGE
Sbjct: 61  VTAKTEDPKSKSDCYGVFCLTYDLKAEEETTSWKKLINVSVSGAAGMISNHLLFKLASGE 120

Query: 121 VFGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEWALLIG 180
           VFGPDQPIALKLLGSERS QALEGVAMELEDSLFPLLREVVISIDP+EVFQDAEWALLIG
Sbjct: 121 VFGPDQPIALKLLGSERSLQALEGVAMELEDSLFPLLREVVISIDPHEVFQDAEWALLIG 180

Query: 181 AKPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKNAPKIP 240
           AKPRGPGMERA LLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALIC+KNAPKIP
Sbjct: 181 AKPRGPGMERAALLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICLKNAPKIP 240

Query: 241 AKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGLPVKEV 300
           AKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNA+INGLPVKEV
Sbjct: 241 AKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNAKINGLPVKEV 300

Query: 301 IKDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWFSSGVY 360
           IKDHRWLEEEFTEKVQKRGG+LIEKWGRSSAASTAVSIVDAIRSL+TPTPEGDWFSSGVY
Sbjct: 301 IKDHRWLEEEFTEKVQKRGGLLIEKWGRSSAASTAVSIVDAIRSLVTPTPEGDWFSSGVY 360

Query: 361 TTGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLT 420
           TTGNPYGIA+DIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLT
Sbjct: 361 TTGNPYGIADDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRCVAHLT 420

Query: 421 GEGIAVCDLPEDTMLPGEM 439
           GEGIAVCDLP DTMLPGEM
Sbjct: 421 GEGIAVCDLPGDTMLPGEM 437

BLAST of Tan0020410 vs. TAIR 10
Match: AT5G58330.2 (lactate/malate dehydrogenase family protein )

HSP 1 Score: 697.2 bits (1798), Expect = 8.4e-201
Identity = 359/445 (80.67%), Postives = 388/445 (87.19%), Query Frame = 0

Query: 1   MAVAELSSSLKTE--LNLSSKIVLQSN-QFSNHRRCSFRP--FYRTRSARITCSIAPNQV 60
           MA+AELS+   T   LN SS++ L S    SNH R    P     T +++I+CS++ NQ 
Sbjct: 1   MAMAELSTPKTTSPFLNSSSRLRLSSKLHLSNHFRHLLLPPLHTTTPNSKISCSVSQNQ- 60

Query: 61  EAAPVAAKTED-PKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFK 120
             APVA +     K+K +C+GVFCLTYDLKAEEET SWKK+IN+AVSGAAGMISNHLLFK
Sbjct: 61  --APVAVQENGLVKTKKECYGVFCLTYDLKAEEETRSWKKLINIAVSGAAGMISNHLLFK 120

Query: 121 LASGEVFGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEW 180
           LASGEVFGPDQPIALKLLGSERS QALEGVAMELEDSLFPLLREV I  DP EVFQD EW
Sbjct: 121 LASGEVFGPDQPIALKLLGSERSIQALEGVAMELEDSLFPLLREVDIGTDPNEVFQDVEW 180

Query: 181 ALLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKN 240
           A+LIGAKPRGPGMERA LLDINGQIFAEQGKALN  ASPNVKV+VVGNPCNTNALIC+KN
Sbjct: 181 AILIGAKPRGPGMERADLLDINGQIFAEQGKALNKAASPNVKVLVVGNPCNTNALICLKN 240

Query: 241 APKIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGL 300
           AP IPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGL
Sbjct: 241 APNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGL 300

Query: 301 PVKEVIKDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWF 360
           PVKEVI DH+WLEE FTE VQKRGG+LI+KWGRSSAASTAVSIVDAI+SL+TPTPEGDWF
Sbjct: 301 PVKEVITDHKWLEEGFTESVQKRGGLLIQKWGRSSAASTAVSIVDAIKSLVTPTPEGDWF 360

Query: 361 SSGVYTTGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRC 420
           S+GVYT GNPYGI E +VFSMPCRSKGDGDYELVKDV  DDYLR+RIAK+EAELLAEKRC
Sbjct: 361 STGVYTDGNPYGIEEGLVFSMPCRSKGDGDYELVKDVEIDDYLRQRIAKSEAELLAEKRC 420

Query: 421 VAHLTGEGIAVCDL-PEDTMLPGEM 439
           VAHLTGEGIA CDL P DTMLPGE+
Sbjct: 421 VAHLTGEGIAYCDLGPVDTMLPGEV 442

BLAST of Tan0020410 vs. TAIR 10
Match: AT5G58330.1 (lactate/malate dehydrogenase family protein )

HSP 1 Score: 695.3 bits (1793), Expect = 3.2e-200
Identity = 358/445 (80.45%), Postives = 387/445 (86.97%), Query Frame = 0

Query: 1   MAVAELSSSLKTE--LNLSSKIVLQSN-QFSNHRRCSFRP--FYRTRSARITCSIAPNQV 60
           MA+AELS+   T   LN SS++ L S    SNH R    P     T +++I+CS++ N  
Sbjct: 1   MAMAELSTPKTTSPFLNSSSRLRLSSKLHLSNHFRHLLLPPLHTTTPNSKISCSVSQN-- 60

Query: 61  EAAPVAAKTED-PKSKSDCFGVFCLTYDLKAEEETTSWKKMINVAVSGAAGMISNHLLFK 120
             APVA +     K+K +C+GVFCLTYDLKAEEET SWKK+IN+AVSGAAGMISNHLLFK
Sbjct: 61  SQAPVAVQENGLVKTKKECYGVFCLTYDLKAEEETRSWKKLINIAVSGAAGMISNHLLFK 120

Query: 121 LASGEVFGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDPYEVFQDAEW 180
           LASGEVFGPDQPIALKLLGSERS QALEGVAMELEDSLFPLLREV I  DP EVFQD EW
Sbjct: 121 LASGEVFGPDQPIALKLLGSERSIQALEGVAMELEDSLFPLLREVDIGTDPNEVFQDVEW 180

Query: 181 ALLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCNTNALICMKN 240
           A+LIGAKPRGPGMERA LLDINGQIFAEQGKALN  ASPNVKV+VVGNPCNTNALIC+KN
Sbjct: 181 AILIGAKPRGPGMERADLLDINGQIFAEQGKALNKAASPNVKVLVVGNPCNTNALICLKN 240

Query: 241 APKIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGL 300
           AP IPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGL
Sbjct: 241 APNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPDFLNARINGL 300

Query: 301 PVKEVIKDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLITPTPEGDWF 360
           PVKEVI DH+WLEE FTE VQKRGG+LI+KWGRSSAASTAVSIVDAI+SL+TPTPEGDWF
Sbjct: 301 PVKEVITDHKWLEEGFTESVQKRGGLLIQKWGRSSAASTAVSIVDAIKSLVTPTPEGDWF 360

Query: 361 SSGVYTTGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTEAELLAEKRC 420
           S+GVYT GNPYGI E +VFSMPCRSKGDGDYELVKDV  DDYLR+RIAK+EAELLAEKRC
Sbjct: 361 STGVYTDGNPYGIEEGLVFSMPCRSKGDGDYELVKDVEIDDYLRQRIAKSEAELLAEKRC 420

Query: 421 VAHLTGEGIAVCDL-PEDTMLPGEM 439
           VAHLTGEGIA CDL P DTMLPGE+
Sbjct: 421 VAHLTGEGIAYCDLGPVDTMLPGEV 443

BLAST of Tan0020410 vs. TAIR 10
Match: AT5G58330.3 (lactate/malate dehydrogenase family protein )

HSP 1 Score: 609.8 bits (1571), Expect = 1.8e-174
Identity = 300/334 (89.82%), Postives = 313/334 (93.71%), Query Frame = 0

Query: 106 MISNHLLFKLASGEVFGPDQPIALKLLGSERSFQALEGVAMELEDSLFPLLREVVISIDP 165
           MISNHLLFKLASGEVFGPDQPIALKLLGSERS QALEGVAMELEDSLFPLLREV I  DP
Sbjct: 1   MISNHLLFKLASGEVFGPDQPIALKLLGSERSIQALEGVAMELEDSLFPLLREVDIGTDP 60

Query: 166 YEVFQDAEWALLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVASPNVKVIVVGNPCN 225
            EVFQD EWA+LIGAKPRGPGMERA LLDINGQIFAEQGKALN  ASPNVKV+VVGNPCN
Sbjct: 61  NEVFQDVEWAILIGAKPRGPGMERADLLDINGQIFAEQGKALNKAASPNVKVLVVGNPCN 120

Query: 226 TNALICMKNAPKIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPD 285
           TNALIC+KNAP IPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPD
Sbjct: 121 TNALICLKNAPNIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNMTIWGNHSTTQVPD 180

Query: 286 FLNARINGLPVKEVIKDHRWLEEEFTEKVQKRGGVLIEKWGRSSAASTAVSIVDAIRSLI 345
           FLNARINGLPVKEVI DH+WLEE FTE VQKRGG+LI+KWGRSSAASTAVSIVDAI+SL+
Sbjct: 181 FLNARINGLPVKEVITDHKWLEEGFTESVQKRGGLLIQKWGRSSAASTAVSIVDAIKSLV 240

Query: 346 TPTPEGDWFSSGVYTTGNPYGIAEDIVFSMPCRSKGDGDYELVKDVIFDDYLRKRIAKTE 405
           TPTPEGDWFS+GVYT GNPYGI E +VFSMPCRSKGDGDYELVKDV  DDYLR+RIAK+E
Sbjct: 241 TPTPEGDWFSTGVYTDGNPYGIEEGLVFSMPCRSKGDGDYELVKDVEIDDYLRQRIAKSE 300

Query: 406 AELLAEKRCVAHLTGEGIAVCDL-PEDTMLPGEM 439
           AELLAEKRCVAHLTGEGIA CDL P DTMLPGE+
Sbjct: 301 AELLAEKRCVAHLTGEGIAYCDLGPVDTMLPGEV 334

BLAST of Tan0020410 vs. TAIR 10
Match: AT1G04410.1 (Lactate/malate dehydrogenase family protein )

HSP 1 Score: 238.0 bits (606), Expect = 1.4e-62
Identity = 135/324 (41.67%), Postives = 190/324 (58.64%), Query Frame = 0

Query: 93  KKMINVAVSGAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSFQALEGVAMELEDSL 152
           K+ + V V+GAAG I   L+  +A G + G DQP+ L +L    + +AL GV MEL D+ 
Sbjct: 3   KEPVRVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPPAAEALNGVKMELIDAA 62

Query: 153 FPLLREVVISIDPYEVFQDAEWALLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVAS 212
           FPLL+ VV + D  E       A+++G  PR  GMER  ++  N  I+  Q  AL   A+
Sbjct: 63  FPLLKGVVATTDAVEGCTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQAAALEKHAA 122

Query: 213 PNVKVIVVGNPCNTNALICMKNAPKIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNM 272
           PN KV+VV NP NTNALI  + AP IP KN   LTRLD NRA  Q++ +  V    V N+
Sbjct: 123 PNCKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLSVPVSDVKNV 182

Query: 273 TIWGNHSTTQVPDFLNARI----NGLPVKEVIKDHRWLEEEFTEKVQKRGGVLIEKWGRS 332
            IWGNHS++Q PD  +A++       PV+E++KD  WL+ EF   VQ+RG  +I+    S
Sbjct: 183 IIWGNHSSSQYPDVNHAKVQTSSGEKPVRELVKDDAWLDGEFISTVQQRGAAIIKARKLS 242

Query: 333 SAASTAVSIVDAIRSLITPTPEGDWFSSGVYTTGNPYGIAEDIVFSMPCRSKGDGDYELV 392
           SA S A S  D IR  +  TPEG + S GVY+ G+ Y +   +++S P   + +GD+ +V
Sbjct: 243 SALSAASSACDHIRDWVLGTPEGTFVSMGVYSDGS-YSVPSGLIYSFPVTCR-NGDWSIV 302

Query: 393 KDVIFDDYLRKRIAKTEAELLAEK 413
           + +  D+  RK++  T  EL  EK
Sbjct: 303 QGLPIDEVSRKKMDLTAEELKEEK 324

BLAST of Tan0020410 vs. TAIR 10
Match: AT5G43330.1 (Lactate/malate dehydrogenase family protein )

HSP 1 Score: 236.1 bits (601), Expect = 5.3e-62
Identity = 135/324 (41.67%), Postives = 188/324 (58.02%), Query Frame = 0

Query: 93  KKMINVAVSGAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSFQALEGVAMELEDSL 152
           K+ + V V+GAAG I   L+  +A G + G DQP+ L +L    + +AL GV MEL D+ 
Sbjct: 3   KEPVRVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPFAAEALNGVKMELVDAA 62

Query: 153 FPLLREVVISIDPYEVFQDAEWALLIGAKPRGPGMERAGLLDINGQIFAEQGKALNAVAS 212
           FPLL+ VV + D  E       A+++G  PR  GMER  ++  N  I+  Q  AL   A+
Sbjct: 63  FPLLKGVVATTDAVEACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKHAA 122

Query: 213 PNVKVIVVGNPCNTNALICMKNAPKIPAKNFHALTRLDENRAKCQLALKAGVFYDKVSNM 272
           PN KV+VV NP NTNALI  + AP IP KN   LTRLD NRA  Q++ +  V    V N+
Sbjct: 123 PNCKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQVSERLSVPVSDVKNV 182

Query: 273 TIWGNHSTTQVPDFLNA----RINGLPVKEVIKDHRWLEEEFTEKVQKRGGVLIEKWGRS 332
            IWGNHS+TQ PD  +A     +   PV+E++K+  WL  EF   VQ+RG  +I+    S
Sbjct: 183 IIWGNHSSTQYPDVNHATVKTSVGEKPVRELVKNDEWLNGEFISTVQQRGAAIIKARKLS 242

Query: 333 SAASTAVSIVDAIRSLITPTPEGDWFSSGVYTTGNPYGIAEDIVFSMPCRSKGDGDYELV 392
           SA S A S  D IR  +  TPEG + S GVY+ G+ Y +   +++S P   + +G++ +V
Sbjct: 243 SALSAASSACDHIRDWVVGTPEGTFVSMGVYSDGS-YNVPAGLIYSFPVTCR-NGEWTIV 302

Query: 393 KDVIFDDYLRKRIAKTEAELLAEK 413
           + +  DD  RK++  T  EL  EK
Sbjct: 303 QGLPIDDASRKKMDLTAEELKEEK 324

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O489021.1e-21885.65Malate dehydrogenase [NADP], chloroplastic OS=Medicago sativa OX=3879 GN=MDH1 PE... [more]
P215282.6e-21584.42Malate dehydrogenase [NADP], chloroplastic OS=Pisum sativum OX=3888 PE=1 SV=2[more]
Q051457.2e-20581.22Malate dehydrogenase [NADP], chloroplastic OS=Mesembryanthemum crystallinum OX=3... [more]
Q8H1E24.5e-19980.45Malate dehydrogenase [NADP], chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At... [more]
P464895.0e-19878.73Malate dehydrogenase [NADP], chloroplastic OS=Flaveria bidentis OX=4224 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
XP_038891999.12.6e-24497.26malate dehydrogenase [NADP], chloroplastic [Benincasa hispida][more]
XP_022968193.14.7e-23893.84malate dehydrogenase [NADP], chloroplastic [Cucurbita maxima][more]
XP_022945464.11.1e-23793.84malate dehydrogenase [NADP], chloroplastic [Cucurbita moschata][more]
XP_023541500.11.4e-23793.84malate dehydrogenase [NADP], chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022139318.11.5e-23694.06malate dehydrogenase [NADP], chloroplastic [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1HXB52.3e-23893.84Malate dehydrogenase [NADP], chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1G1165.1e-23893.84Malate dehydrogenase [NADP], chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1CFD87.4e-23794.06Malate dehydrogenase [NADP], chloroplastic OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A0A0KTH81.2e-23493.17Malate dehydrogenase [NADP], chloroplastic OS=Cucumis sativus OX=3659 GN=Csa_5G6... [more]
A0A5D3CB643.4e-23493.17Malate dehydrogenase [NADP], chloroplastic OS=Cucumis melo var. makuwa OX=119469... [more]
Match NameE-valueIdentityDescription
AT5G58330.28.4e-20180.67lactate/malate dehydrogenase family protein [more]
AT5G58330.13.2e-20080.45lactate/malate dehydrogenase family protein [more]
AT5G58330.31.8e-17489.82lactate/malate dehydrogenase family protein [more]
AT1G04410.11.4e-6241.67Lactate/malate dehydrogenase family protein [more]
AT5G43330.15.3e-6241.67Lactate/malate dehydrogenase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001236Lactate/malate dehydrogenase, N-terminalPFAMPF00056Ldh_1_Ncoord: 97..244
e-value: 2.1E-34
score: 118.6
IPR010945Malate dehydrogenase, type 2TIGRFAMTIGR01759TIGR01759coord: 94..414
e-value: 1.3E-142
score: 472.6
IPR010945Malate dehydrogenase, type 2PANTHERPTHR23382MALATE DEHYDROGENASEcoord: 1..437
IPR011273Malate dehydrogenase, NADP-dependent, plantsTIGRFAMTIGR01757TIGR01757coord: 58..437
e-value: 6.8E-224
score: 740.5
IPR022383Lactate/malate dehydrogenase, C-terminalPFAMPF02866Ldh_1_Ccoord: 247..417
e-value: 5.9E-37
score: 127.2
NoneNo IPR availableGENE3D3.40.50.720coord: 93..245
e-value: 7.1E-55
score: 187.0
NoneNo IPR availablePIRSRPIRSR000102-1PIRSR000102-1coord: 94..415
e-value: 3.5E-56
score: 188.4
NoneNo IPR availablePIRSRPIRSR000102-2PIRSR000102-2coord: 94..415
e-value: 3.5E-56
score: 188.4
NoneNo IPR availablePANTHERPTHR23382:SF18MALATE DEHYDROGENASEcoord: 1..437
NoneNo IPR availableCDDcd01338MDH_choloroplast_likecoord: 94..417
e-value: 0.0
score: 601.114
IPR015955Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminalGENE3D3.90.110.10coord: 246..418
e-value: 2.5E-55
score: 188.6
IPR015955Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminalSUPERFAMILY56327LDH C-terminal domain-likecoord: 247..435
IPR001252Malate dehydrogenase, active sitePROSITEPS00068MDHcoord: 246..258
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 81..245

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0020410.1Tan0020410.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0006108 malate metabolic process
biological_process GO:0006734 NADH metabolic process
biological_process GO:0006107 oxaloacetate metabolic process
biological_process GO:0051775 response to redox state
biological_process GO:0006099 tricarboxylic acid cycle
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0030060 L-malate dehydrogenase activity
molecular_function GO:0046554 malate dehydrogenase (NADP+) activity
molecular_function GO:0008746 NAD(P)+ transhydrogenase activity
molecular_function GO:0003824 catalytic activity
molecular_function GO:0016615 malate dehydrogenase activity
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor