Tan0020281 (gene) Snake gourd v1

Overview


Name	Tan0020281
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	RING-type E3 ubiquitin transferase
Location	LG09: 63358942 .. 63359514 (+)
RNA-Seq Expression	Tan0020281
Synteny	Tan0020281

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTGATTCAGCTTCAACCTCTTTCGCTCAGCCAACGCCTCATCCTGCAAAATCAAGCCTACCCATGTTGTATTATGGCCTTGTGGTCGTCGGAACGGCGGCGATTGTGTTGGTTCTTTACAATTTCATCGTTATCAAGTGGTGCTCCGACAACCGCCACCGTCGAGCTTCACCGGCGCTGGCCGGGAGTTATGCAGAGATGGTGAATTCAAGCCGCAGCTTCCAGAGCTCAAATAGGAACTTGTTGTCCAGCTTCAAATTCAAGAAAGGGTCCACGGCCACAACGACGGCGACAACGGCGGTGGCAGGGGAAGAAGAAGGGAATGGGAATGAATGTGCGGTTTGTTTGTCAGCGTTTGAGGAAGGAGAAGAGGTGAAGAAACTGCCAAGATGCACACATATGTTTCATGCCCCTTGTATAGACATGTGGCTTTACTCTCATTCCGATTGCCCCCTCTGCCGTGCTCCGGTGGCGGTGCCGGGGCTGTGCCAACACCACACGGCAGCAGCGGCGGCGGAGCAACCGGAGAATTCAGGGCATGGGTTGTTGGAATCCGGCAACTTGGTTTAA

mRNA sequence

ATGGGTGATTCAGCTTCAACCTCTTTCGCTCAGCCAACGCCTCATCCTGCAAAATCAAGCCTACCCATGTTGTATTATGGCCTTGTGGTCGTCGGAACGGCGGCGATTGTGTTGGTTCTTTACAATTTCATCGTTATCAAGTGGTGCTCCGACAACCGCCACCGTCGAGCTTCACCGGCGCTGGCCGGGAGTTATGCAGAGATGGTGAATTCAAGCCGCAGCTTCCAGAGCTCAAATAGGAACTTGTTGTCCAGCTTCAAATTCAAGAAAGGGTCCACGGCCACAACGACGGCGACAACGGCGGTGGCAGGGGAAGAAGAAGGGAATGGGAATGAATGTGCGGTTTGTTTGTCAGCGTTTGAGGAAGGAGAAGAGGTGAAGAAACTGCCAAGATGCACACATATGTTTCATGCCCCTTGTATAGACATGTGGCTTTACTCTCATTCCGATTGCCCCCTCTGCCGTGCTCCGGTGGCGGTGCCGGGGCTGTGCCAACACCACACGGCAGCAGCGGCGGCGGAGCAACCGGAGAATTCAGGGCATGGGTTGTTGGAATCCGGCAACTTGGTTTAA

Coding sequence (CDS)

ATGGGTGATTCAGCTTCAACCTCTTTCGCTCAGCCAACGCCTCATCCTGCAAAATCAAGCCTACCCATGTTGTATTATGGCCTTGTGGTCGTCGGAACGGCGGCGATTGTGTTGGTTCTTTACAATTTCATCGTTATCAAGTGGTGCTCCGACAACCGCCACCGTCGAGCTTCACCGGCGCTGGCCGGGAGTTATGCAGAGATGGTGAATTCAAGCCGCAGCTTCCAGAGCTCAAATAGGAACTTGTTGTCCAGCTTCAAATTCAAGAAAGGGTCCACGGCCACAACGACGGCGACAACGGCGGTGGCAGGGGAAGAAGAAGGGAATGGGAATGAATGTGCGGTTTGTTTGTCAGCGTTTGAGGAAGGAGAAGAGGTGAAGAAACTGCCAAGATGCACACATATGTTTCATGCCCCTTGTATAGACATGTGGCTTTACTCTCATTCCGATTGCCCCCTCTGCCGTGCTCCGGTGGCGGTGCCGGGGCTGTGCCAACACCACACGGCAGCAGCGGCGGCGGAGCAACCGGAGAATTCAGGGCATGGGTTGTTGGAATCCGGCAACTTGGTTTAA

Protein sequence

MGDSASTSFAQPTPHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHRRASPALAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTATTTATTAVAGEEEGNGNECAVCLSAFEEGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAVPGLCQHHTAAAAAEQPENSGHGLLESGNLV

Homology

[Close]

BLAST of Tan0020281 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 4.9e-17
Identity = 50/140 (35.71%), Postives = 77/140 (55.00%), Query Frame = 0


Query: 17  AKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHRRASPALAGSYAEMVNSSRSFQ 76
           + SS   L   L+ + T+A++LV Y  ++ K+C  +    +S  L  ++    N    F 
Sbjct: 50  SSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLNH----NGEGFFS 109

Query: 77  SSNRNLLSSFKFKKGSTATTTATTAVAGEEEGNGNECAVCLSAFEEGEEVKKLPRCTHMF 136
           S+ R   +     +    + T     +G+   +G++C+VCLS FEE E ++ LP+C H F
Sbjct: 110 STQRISTNGDGLNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAF 169

Query: 137 HAPCIDMWLYSHSDCPLCRA 157
           H PCID WL SHS+CPLCRA
Sbjct: 170 HLPCIDTWLKSHSNCPLCRA 185

[Close]

BLAST of Tan0020281 vs. ExPASy Swiss-Prot
Match: Q9LRB7 (E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5.1 PE=1 SV=1)

HSP 1 Score: 87.4 bits (215), Expect = 1.9e-16
Identity = 60/172 (34.88%), Postives = 78/172 (45.35%), Query Frame = 0


Query: 1   MGDSASTSFAQPTPHPAKSSLPMLYYGLVVVGTAAIVLVLYN--FIVIKWCSDNRHRRAS 60
           M +S+S+S   P   PA     M   G+  V    IV       F++++   D R R  +
Sbjct: 12  MDESSSSSSPSPVSAPA-GQAAMTAGGIATVAAVLIVFAALTLAFVLLQCYCDERRRAVT 71

Query: 61  PALAGSYAEMVNSSRSFQSS---------NRNLLSSFKFKKGSTATTTATTAVAGEEEGN 120
                         R   S          +  +L S      S +T  A      EE+ +
Sbjct: 72  TTSTSGRGRRPRPRRRSGSGGDGGTGGGVDPEVLRSLPVTVYSRSTAAAAAKEEEEEDDD 131

Query: 121 GNECAVCLSAFEEGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAVP 162
           G ECAVCL+  E+GEE + LPRC H FHA C+DMWL SHS CPLCR  V VP
Sbjct: 132 GVECAVCLAELEDGEEARFLPRCGHGFHAECVDMWLGSHSTCPLCRLTVVVP 182

[Close]

BLAST of Tan0020281 vs. ExPASy Swiss-Prot
Match: P93823 (RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.4e-16
Identity = 65/191 (34.03%), Postives = 95/191 (49.74%), Query Frame = 0


Query: 6   STSFAQPT-PHPAKS--SLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSD-------NRHR 65
           ST+F  P  P  + S  + P+L   ++ +   A +LV Y   VIK C +        R R
Sbjct: 23  STTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQIDIFRRRR 82

Query: 66  RAS---PALAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTATTTATTAVAGEEE--GNG 125
           R+S   P +  S  E+   +R    S    +  FKFKK           VAGEE+   N 
Sbjct: 83  RSSDQNPLMIYSPHEV---NRGLDESAIRAIPVFKFKK--------RDVVAGEEDQSKNS 142

Query: 126 NECAVCLSAFEEGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAVPGLCQHHTAA 182
            EC+VCL+ F+E E+++ +P C H+FH  CID+WL  +++CPLCR  V+          +
Sbjct: 143 QECSVCLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVSCEASFTLDLIS 202

[Close]

BLAST of Tan0020281 vs. ExPASy Swiss-Prot
Match: Q9SRQ8 (RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2)

HSP 1 Score: 83.2 bits (204), Expect = 3.5e-15
Identity = 51/161 (31.68%), Postives = 76/161 (47.20%), Query Frame = 0


Query: 17  AKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHRRASPALAGSYAEMVNSSRSFQ 76
           + S    L   L+ +  +A +LV Y  ++ K+C   RH  +S + A      ++S  ++Q
Sbjct: 52  SSSDFSPLLIALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAA--INRISSDYTWQ 111

Query: 77  SSNRNLLSSFKFKKGSTATTTATTAVAGEEEG---------------------NGNECAV 136
            +N N          + AT    T   G  +G                       ++C+V
Sbjct: 112 GTNNN--------NNNGATNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSV 171

Query: 137 CLSAFEEGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRA 157
           CLS F+E E ++ LP+C H FH PCID WL SHS+CPLCRA
Sbjct: 172 CLSEFQENESLRLLPKCNHAFHVPCIDTWLKSHSNCPLCRA 202

[Close]

BLAST of Tan0020281 vs. ExPASy Swiss-Prot
Match: Q9LSW9 (RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 7.8e-15
Identity = 61/191 (31.94%), Postives = 88/191 (46.07%), Query Frame = 0


Query: 8   SFAQPTPHP-------AKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWC----------- 67
           SF  P P P         +S P+L   ++ +   A +LV Y   VIK C           
Sbjct: 14  SFPPPPPPPIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGR 73

Query: 68  -SDNRHRRASPALAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTATTTATTAVA-GEEE 127
            S +R RR        Y+  +  SR    S    +  FKFKK         T     EEE
Sbjct: 74  FSLSRRRRNDQDPLMVYSPELR-SRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEE 133

Query: 128 GNGNECAVCLSAFEEGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAVPGLCQHH 179
               EC+VCLS F++ E+++ +P C+H+FH  CID+WL ++++CPLCR  V+        
Sbjct: 134 KRSQECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVSCDTSFPPD 193

[Close]

BLAST of Tan0020281 vs. NCBI nr
Match: XP_022968369.1 (RING-H2 finger protein ATL52-like [Cucurbita maxima])

HSP 1 Score: 315.1 bits (806), Expect = 4.2e-82
Identity = 160/190 (84.21%), Postives = 165/190 (86.84%), Query Frame = 0


Query: 1   MGDSASTSFAQPTPHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHRRASPA 60
           MGDSAST  AQPT HPAKSSLPMLYYGLVV+GTA IVLVLYN I+IKWCSDN H +ASPA
Sbjct: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDNSHGQASPA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTATTTATTAVAGEEEGNGNECAVCLSAF 120
           LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTA  TAT     EEEG GNECAVCLSAF
Sbjct: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTAKATATI----EEEGIGNECAVCLSAF 120

Query: 121 EEGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAVPGLCQHHTAAAAAEQPENSG 180
           E+GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAP   P LCQ HTA  AAEQ  NSG
Sbjct: 121 EDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPAMAPALCQRHTAEDAAEQQVNSG 180

Query: 181 HGLLESGNLV 191
           HGLLES +LV
Sbjct: 181 HGLLESSSLV 186

[Close]

BLAST of Tan0020281 vs. NCBI nr
Match: XP_038879803.1 (RING-H2 finger protein ATL39-like [Benincasa hispida])

HSP 1 Score: 298.1 bits (762), Expect = 5.3e-77
Identity = 156/190 (82.11%), Postives = 162/190 (85.26%), Query Frame = 0


Query: 1   MGDSASTSFAQPTPHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHRRASPA 60
           M DS+STSFAQPTPHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHRRASPA
Sbjct: 1   MSDSSSTSFAQPTPHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHRRASPA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTATTTATTAVAGEEEGNGNECAVCLSAF 120
           LAGSYAEM+NSSRSF SS    L SFKFKKGST       A A EEEG GNECAVCLSAF
Sbjct: 61  LAGSYAEMMNSSRSFHSS----LCSFKFKKGST-----VAAAAAEEEGYGNECAVCLSAF 120

Query: 121 EEGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAVPGLCQHHTAAAAAEQPENSG 180
           E+G+EVKKLPRCTHMFHA CIDMWL+SHSDCPLCRAPV   GL QH    A AEQ ENSG
Sbjct: 121 EDGDEVKKLPRCTHMFHASCIDMWLFSHSDCPLCRAPVVAQGLSQHQ---AMAEQEENSG 178

Query: 181 HGLLESGNLV 191
           HGLLESG+LV
Sbjct: 181 HGLLESGDLV 178

[Close]

BLAST of Tan0020281 vs. NCBI nr
Match: KAG6590024.1 (RING-H2 finger protein ATL1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7023686.1 RING-H2 finger protein ATL1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 296.2 bits (757), Expect = 2.0e-76
Identity = 156/190 (82.11%), Postives = 159/190 (83.68%), Query Frame = 0


Query: 1   MGDSASTSFAQPTPHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHRRASPA 60
           M DSAS  FAQPT HPAKSSLPMLYYGLVV+GTAAIVLVLYNFIVI WCSDNR  RASPA
Sbjct: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTATTTATTAVAGEEEGNGNECAVCLSAF 120
           LAGSYAEMVNSSRS QSSNRNLL SFKFKKGS A  TA    A EEEGNGNEC VCLSAF
Sbjct: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAAAATA----AAEEEGNGNECVVCLSAF 120

Query: 121 EEGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAVPGLCQHHTAAAAAEQPENSG 180
           EEGEEVKKLPRC HMFHA CIDMWL+SH DCPLCRAPVAVPGL +  T   AAEQ  NSG
Sbjct: 121 EEGEEVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQT---AAEQQGNSG 180

Query: 181 HGLLESGNLV 191
           HGL ESG LV
Sbjct: 181 HGLPESGGLV 183

[Close]

BLAST of Tan0020281 vs. NCBI nr
Match: KAG6588064.1 (RING-H2 finger protein ATL52, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021960.1 RING-H2 finger protein ATL52, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 295.0 bits (754), Expect = 4.5e-76
Identity = 148/174 (85.06%), Postives = 154/174 (88.51%), Query Frame = 0


Query: 1   MGDSASTSFAQPTPHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHRRASPA 60
           MGDSAST  AQPT HPAKSSLPMLYYGLVV+GTA IVLVLYN I+IKWCSD+ H +ASPA
Sbjct: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASPA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTATTTATTAVAGEEEGNGNECAVCLSAF 120
           LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGS AT  AT  +  EEEG GNECAVCLSAF
Sbjct: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATI--EEEGIGNECAVCLSAF 120

Query: 121 EEGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAVPGLCQHHTAAAAAE 175
           E+GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPV  P LCQ HTA AAAE
Sbjct: 121 EDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTAEAAAE 172

[Close]

BLAST of Tan0020281 vs. NCBI nr
Match: XP_022960602.1 (E3 ubiquitin-protein ligase EL5-like [Cucurbita moschata])

HSP 1 Score: 294.3 bits (752), Expect = 7.7e-76
Identity = 155/190 (81.58%), Postives = 158/190 (83.16%), Query Frame = 0


Query: 1   MGDSASTSFAQPTPHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHRRASPA 60
           M DSAS  FAQPT HPAKSSLPMLYYGLVV+GTAAIVLVLYNFIVI WCSDNR  RASPA
Sbjct: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTATTTATTAVAGEEEGNGNECAVCLSAF 120
           LAGSYAEMVNSSRS QSSNRNLL SFKFKKGS A      A A EEEGNGNEC VCLSAF
Sbjct: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAA------AAAAEEEGNGNECVVCLSAF 120

Query: 121 EEGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAVPGLCQHHTAAAAAEQPENSG 180
           EEGEEVKKLPRC HMFHA CIDMWL+SH DCPLCRAPVAVPGL +  T   AAEQ  NSG
Sbjct: 121 EEGEEVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQT---AAEQQGNSG 180

Query: 181 HGLLESGNLV 191
           HGL ESG LV
Sbjct: 181 HGLPESGGLV 181

[Close]

BLAST of Tan0020281 vs. ExPASy TrEMBL
Match: A0A6J1HZG2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467629 PE=3 SV=1)

HSP 1 Score: 315.1 bits (806), Expect = 2.0e-82
Identity = 160/190 (84.21%), Postives = 165/190 (86.84%), Query Frame = 0


Query: 1   MGDSASTSFAQPTPHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHRRASPA 60
           MGDSAST  AQPT HPAKSSLPMLYYGLVV+GTA IVLVLYN I+IKWCSDN H +ASPA
Sbjct: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDNSHGQASPA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTATTTATTAVAGEEEGNGNECAVCLSAF 120
           LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTA  TAT     EEEG GNECAVCLSAF
Sbjct: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTAKATATI----EEEGIGNECAVCLSAF 120

Query: 121 EEGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAVPGLCQHHTAAAAAEQPENSG 180
           E+GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAP   P LCQ HTA  AAEQ  NSG
Sbjct: 121 EDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPAMAPALCQRHTAEDAAEQQVNSG 180

Query: 181 HGLLESGNLV 191
           HGLLES +LV
Sbjct: 181 HGLLESSSLV 186

[Close]

BLAST of Tan0020281 vs. ExPASy TrEMBL
Match: A0A6J1H7W2 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461337 PE=3 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 3.7e-76
Identity = 155/190 (81.58%), Postives = 158/190 (83.16%), Query Frame = 0


Query: 1   MGDSASTSFAQPTPHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHRRASPA 60
           M DSAS  FAQPT HPAKSSLPMLYYGLVV+GTAAIVLVLYNFIVI WCSDNR  RASPA
Sbjct: 1   MSDSASNYFAQPTSHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTATTTATTAVAGEEEGNGNECAVCLSAF 120
           LAGSYAEMVNSSRS QSSNRNLL SFKFKKGS A      A A EEEGNGNEC VCLSAF
Sbjct: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGSAA------AAAAEEEGNGNECVVCLSAF 120

Query: 121 EEGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAVPGLCQHHTAAAAAEQPENSG 180
           EEGEEVKKLPRC HMFHA CIDMWL+SH DCPLCRAPVAVPGL +  T   AAEQ  NSG
Sbjct: 121 EEGEEVKKLPRCMHMFHACCIDMWLFSHCDCPLCRAPVAVPGLSKRQT---AAEQQGNSG 180

Query: 181 HGLLESGNLV 191
           HGL ESG LV
Sbjct: 181 HGLPESGGLV 181

[Close]

BLAST of Tan0020281 vs. ExPASy TrEMBL
Match: A0A6J1D0Z7 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111016600 PE=3 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 1.4e-75
Identity = 152/188 (80.85%), Postives = 162/188 (86.17%), Query Frame = 0


Query: 4   SASTSFAQPTPHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHRRASPALAG 63
           S+ST F QPTPHPAKSSLPMLYYGLVVVGTAAIVLV+YNFIVI+WCSD R  R SP +A 
Sbjct: 6   SSSTPFTQPTPHPAKSSLPMLYYGLVVVGTAAIVLVVYNFIVIRWCSD-RRGRGSPGMAR 65

Query: 64  SYAEMVNSSRSFQSSNRNLLSSFKFKKGSTATTTATTAVAGEEEGNGNECAVCLSAFEEG 123
           SYAEM++SS SFQSS+RNLLSSF+FKKG     T   A   EEEGNG ECAVCLSAFE+G
Sbjct: 66  SYAEMMSSSGSFQSSSRNLLSSFRFKKG-----TKAAAATAEEEGNGLECAVCLSAFEDG 125

Query: 124 EEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAV-PGLCQHHTAAAAAEQPENSGHG 183
           EEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVA+ PGLCQHHT   AAEQPENSGHG
Sbjct: 126 EEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVALAPGLCQHHT---AAEQPENSGHG 184

Query: 184 LLESGNLV 191
           LLESG LV
Sbjct: 186 LLESGGLV 184

[Close]

BLAST of Tan0020281 vs. ExPASy TrEMBL
Match: A0A6J1JJ05 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485020 PE=3 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 4.1e-75
Identity = 153/190 (80.53%), Postives = 157/190 (82.63%), Query Frame = 0


Query: 1   MGDSASTSFAQPTPHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHRRASPA 60
           M DSAS  F+QPT HPAKSSLPMLYYGLVV+GTAAIVLVLYNFIVI WCSDNR  RASPA
Sbjct: 1   MSDSASNHFSQPTNHPAKSSLPMLYYGLVVIGTAAIVLVLYNFIVIMWCSDNRRGRASPA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTATTTATTAVAGEEEGNGNECAVCLSAF 120
           LAGSYAEMVNSSRS QSSNRNLL SFKFKKGS        A A EEEGNGNEC VCLSAF
Sbjct: 61  LAGSYAEMVNSSRSLQSSNRNLLCSFKFKKGS-------AAAAVEEEGNGNECVVCLSAF 120

Query: 121 EEGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAVPGLCQHHTAAAAAEQPENSG 180
           EEGEEVKKLPRC HMFHA CIDMWL+SH DCPLCRAPVAVPGL +  T   AAEQ  NSG
Sbjct: 121 EEGEEVKKLPRCLHMFHARCIDMWLFSHCDCPLCRAPVAVPGLSKRQT---AAEQQGNSG 180

Query: 181 HGLLESGNLV 191
           HGL ESG LV
Sbjct: 181 HGLPESGGLV 180

[Close]

BLAST of Tan0020281 vs. ExPASy TrEMBL
Match: A0A6J1EGS5 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434176 PE=3 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 7.7e-74
Identity = 143/169 (84.62%), Postives = 149/169 (88.17%), Query Frame = 0


Query: 1   MGDSASTSFAQPTPHPAKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHRRASPA 60
           MGDSAST  AQPT HPAKSSLPMLYYGLVV+GTA IVLVLYN I+IKWCSD+ H +AS A
Sbjct: 1   MGDSASTFIAQPTSHPAKSSLPMLYYGLVVIGTATIVLVLYNLIIIKWCSDDSHGQASTA 60

Query: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTATTTATTAVAGEEEGNGNECAVCLSAF 120
           LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGS AT  AT  +  EEEG GNECAVCLSAF
Sbjct: 61  LAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSMATAAATATI--EEEGIGNECAVCLSAF 120

Query: 121 EEGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAVPGLCQHHTA 170
           E+GEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPV  P LCQ HTA
Sbjct: 121 EDGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVMAPALCQRHTA 167

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BLAST of Tan0020281 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 89.4 bits (220), Expect = 3.5e-18
Identity = 50/140 (35.71%), Postives = 77/140 (55.00%), Query Frame = 0


Query: 17  AKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHRRASPALAGSYAEMVNSSRSFQ 76
           + SS   L   L+ + T+A++LV Y  ++ K+C  +    +S  L  ++    N    F 
Sbjct: 50  SSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLNLNH----NGEGFFS 109

Query: 77  SSNRNLLSSFKFKKGSTATTTATTAVAGEEEGNGNECAVCLSAFEEGEEVKKLPRCTHMF 136
           S+ R   +     +    + T     +G+   +G++C+VCLS FEE E ++ LP+C H F
Sbjct: 110 STQRISTNGDGLNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESLRLLPKCNHAF 169

Query: 137 HAPCIDMWLYSHSDCPLCRA 157
           H PCID WL SHS+CPLCRA
Sbjct: 170 HLPCIDTWLKSHSNCPLCRA 185

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BLAST of Tan0020281 vs. TAIR 10
Match: AT1G04360.1 (RING/U-box superfamily protein )

HSP 1 Score: 87.0 bits (214), Expect = 1.7e-17
Identity = 65/191 (34.03%), Postives = 95/191 (49.74%), Query Frame = 0


Query: 6   STSFAQPT-PHPAKS--SLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSD-------NRHR 65
           ST+F  P  P  + S  + P+L   ++ +   A +LV Y   VIK C +        R R
Sbjct: 23  STTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQIDIFRRRR 82

Query: 66  RAS---PALAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTATTTATTAVAGEEE--GNG 125
           R+S   P +  S  E+   +R    S    +  FKFKK           VAGEE+   N 
Sbjct: 83  RSSDQNPLMIYSPHEV---NRGLDESAIRAIPVFKFKK--------RDVVAGEEDQSKNS 142

Query: 126 NECAVCLSAFEEGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAVPGLCQHHTAA 182
            EC+VCL+ F+E E+++ +P C H+FH  CID+WL  +++CPLCR  V+          +
Sbjct: 143 QECSVCLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVSCEASFTLDLIS 202

[Close]

BLAST of Tan0020281 vs. TAIR 10
Match: AT3G03550.1 (RING/U-box superfamily protein )

HSP 1 Score: 83.2 bits (204), Expect = 2.5e-16
Identity = 51/161 (31.68%), Postives = 76/161 (47.20%), Query Frame = 0


Query: 17  AKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWCSDNRHRRASPALAGSYAEMVNSSRSFQ 76
           + S    L   L+ +  +A +LV Y  ++ K+C   RH  +S + A      ++S  ++Q
Sbjct: 52  SSSDFSPLLIALIGILASAFILVSYYTLISKYCHRRRHNSSSTSAAA--INRISSDYTWQ 111

Query: 77  SSNRNLLSSFKFKKGSTATTTATTAVAGEEEG---------------------NGNECAV 136
            +N N          + AT    T   G  +G                       ++C+V
Sbjct: 112 GTNNN--------NNNGATNPNQTIGGGGGDGLDESLIKSITVYKYRKMDGFVESSDCSV 171

Query: 137 CLSAFEEGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRA 157
           CLS F+E E ++ LP+C H FH PCID WL SHS+CPLCRA
Sbjct: 172 CLSEFQENESLRLLPKCNHAFHVPCIDTWLKSHSNCPLCRA 202

[Close]

BLAST of Tan0020281 vs. TAIR 10
Match: AT5G43420.1 (RING/U-box superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 5.6e-16
Identity = 61/191 (31.94%), Postives = 88/191 (46.07%), Query Frame = 0


Query: 8   SFAQPTPHP-------AKSSLPMLYYGLVVVGTAAIVLVLYNFIVIKWC----------- 67
           SF  P P P         +S P+L   ++ +   A +LV Y   VIK C           
Sbjct: 14  SFPPPPPPPIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGR 73

Query: 68  -SDNRHRRASPALAGSYAEMVNSSRSFQSSNRNLLSSFKFKKGSTATTTATTAVA-GEEE 127
            S +R RR        Y+  +  SR    S    +  FKFKK         T     EEE
Sbjct: 74  FSLSRRRRNDQDPLMVYSPELR-SRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEE 133

Query: 128 GNGNECAVCLSAFEEGEEVKKLPRCTHMFHAPCIDMWLYSHSDCPLCRAPVAVPGLCQHH 179
               EC+VCLS F++ E+++ +P C+H+FH  CID+WL ++++CPLCR  V+        
Sbjct: 134 KRSQECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRVSCDTSFPPD 193

[Close]

BLAST of Tan0020281 vs. TAIR 10
Match: AT2G17450.1 (RING-H2 finger A3A )

HSP 1 Score: 80.1 bits (196), Expect = 2.1e-15
Identity = 39/88 (44.32%), Postives = 51/88 (57.95%), Query Frame = 0


Query: 92  STATTTATTAVAGEEEGNGNECAVCLSAFEEGEEVKKLPRCTHMFHAPCIDMWLYSHSDC 151
           ST T   +T+ A  EEG+  ECA+CL+ F +GEE++ LP C H FH  CID WL S S C
Sbjct: 81  STFTAAESTSGAAAEEGDSTECAICLTDFADGEEIRVLPLCGHSFHVECIDKWLVSRSSC 140

Query: 152 PLCRAPVAVPGLCQHHTAAAAAEQPENS 180
           P CR  +  P  C     A+ AE  + +
Sbjct: 141 PSCRR-ILTPVRCDRCGHASTAEMKDQA 167

The following BLAST results are available for this feature:

BLAST of Tan0020281 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LF64	4.9e-17	35.71	RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1	[more]
Q9LRB7	1.9e-16	34.88	E3 ubiquitin-protein ligase EL5 OS=Oryza sativa subsp. japonica OX=39947 GN=EL5....	[more]
P93823	2.4e-16	34.03	RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1	[more]
Q9SRQ8	3.5e-15	31.68	RING-H2 finger protein ATL51 OS=Arabidopsis thaliana OX=3702 GN=ATL51 PE=2 SV=2	[more]
Q9LSW9	7.8e-15	31.94	RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1	[more]

BLAST of Tan0020281 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022968369.1	4.2e-82	84.21	RING-H2 finger protein ATL52-like [Cucurbita maxima]	[more]
XP_038879803.1	5.3e-77	82.11	RING-H2 finger protein ATL39-like [Benincasa hispida]	[more]
KAG6590024.1	2.0e-76	82.11	RING-H2 finger protein ATL1, partial [Cucurbita argyrosperma subsp. sororia] >KA...	[more]
KAG6588064.1	4.5e-76	85.06	RING-H2 finger protein ATL52, partial [Cucurbita argyrosperma subsp. sororia] >K...	[more]
XP_022960602.1	7.7e-76	81.58	E3 ubiquitin-protein ligase EL5-like [Cucurbita moschata]	[more]

BLAST of Tan0020281 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HZG2	2.0e-82	84.21	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467629 P...	[more]
A0A6J1H7W2	3.7e-76	81.58	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461337...	[more]
A0A6J1D0Z7	1.4e-75	80.85	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101660...	[more]
A0A6J1JJ05	4.1e-75	80.53	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485020 P...	[more]
A0A6J1EGS5	7.7e-74	84.62	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434176...	[more]

BLAST of Tan0020281 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G17600.1	3.5e-18	35.71	RING/U-box superfamily protein	[more]
AT1G04360.1	1.7e-17	34.03	RING/U-box superfamily protein	[more]
AT3G03550.1	2.5e-16	31.68	RING/U-box superfamily protein	[more]
AT5G43420.1	5.6e-16	31.94	RING/U-box superfamily protein	[more]
AT2G17450.1	2.1e-15	44.32	RING-H2 finger A3A	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 113..154 e-value: 1.0E-5 score: 35.0
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 111..155 e-value: 5.9E-13 score: 48.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 113..155 score: 12.046688
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 88..160 e-value: 3.3E-21 score: 76.3
None	No IPR available	PANTHER	PTHR45676:SF84	RING-H2 FINGER PROTEIN ATL52-LIKE	coord: 8..185
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 105..161
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 8..185

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0020281.1	Tan0020281.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane